Reduction in pathogenic biofilms by the photoactive composite of bacterial cellulose and nanochitosan dots under blue and green light
Authors
Zmejoski, Danica Z.Zdravković, Nemanja M.
Budimir Filimonović, Milica D.
Pavlović, Vladimir B.
Butulija, Svetlana V.
Milivojević, Dušan D.
Marković, Zoran M.
Todorović Marković, Biljana M.
Article (Published version)
Metadata
Show full item recordAbstract
In this study, nanochitosan dots (ChiDs) were synthesized using gamma rays and encapsulated
in bacterial cellulose (BC) polymer matrix for antibiofilm potential in photodynamic
therapy. The composites were analyzed for structural changes using SEM, AFM, FTIR, XRD, EPR,
and porosity measurements. Additionally, ChiD release was assessed. The results showed that the
chemical composition remained unaltered, but ChiD agglomerates embedded in BC changed shape
(1.5–2.5 μm). Bacterial cellulose fibers became deformed and interconnected, with increased surface
roughness and porosity and decreased crystallinity. No singlet oxygen formation was observed,
and the total amount of released ChiD was up to 16.10%. Antibiofilm activity was higher under
green light, with reductions ranging from 48 to 57% under blue light and 78 to 85% under green
light. Methicillin-resistant Staphylococcus aureus was the most sensitive strain. The new photoactive
composite hydrogels show promising potential fo...r combating biofilm-related infections.
Keywords:
chitosan nanoparticles / bacterial cellulose / photoactive therapy / antibiofilm / nanocomposite hydrogels / blue light and green lightSource:
Journal of Functional Biomaterials, 2024, 15, 3, 72-Publisher:
- Basel : MDPI
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200017 (University of Belgrade, Institute of Nuclear Sciences 'Vinča', Belgrade-Vinča) (RS-MESTD-inst-2020-200017)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200030 (Scientific Veterinary Institute of Serbia, Belgrade) (RS-MESTD-inst-2020-200030)
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Institution/Community
Naučni institut za veterinarstvo SrbijeTY - JOUR AU - Zmejoski, Danica Z. AU - Zdravković, Nemanja M. AU - Budimir Filimonović, Milica D. AU - Pavlović, Vladimir B. AU - Butulija, Svetlana V. AU - Milivojević, Dušan D. AU - Marković, Zoran M. AU - Todorović Marković, Biljana M. PY - 2024 UR - https://reponivs.nivs.rs/handle/123456789/926 AB - In this study, nanochitosan dots (ChiDs) were synthesized using gamma rays and encapsulated in bacterial cellulose (BC) polymer matrix for antibiofilm potential in photodynamic therapy. The composites were analyzed for structural changes using SEM, AFM, FTIR, XRD, EPR, and porosity measurements. Additionally, ChiD release was assessed. The results showed that the chemical composition remained unaltered, but ChiD agglomerates embedded in BC changed shape (1.5–2.5 μm). Bacterial cellulose fibers became deformed and interconnected, with increased surface roughness and porosity and decreased crystallinity. No singlet oxygen formation was observed, and the total amount of released ChiD was up to 16.10%. Antibiofilm activity was higher under green light, with reductions ranging from 48 to 57% under blue light and 78 to 85% under green light. Methicillin-resistant Staphylococcus aureus was the most sensitive strain. The new photoactive composite hydrogels show promising potential for combating biofilm-related infections. PB - Basel : MDPI T2 - Journal of Functional Biomaterials T1 - Reduction in pathogenic biofilms by the photoactive composite of bacterial cellulose and nanochitosan dots under blue and green light IS - 3 SP - 72 VL - 15 DO - 10.3390/ jfb15030072 ER -
@article{ author = "Zmejoski, Danica Z. and Zdravković, Nemanja M. and Budimir Filimonović, Milica D. and Pavlović, Vladimir B. and Butulija, Svetlana V. and Milivojević, Dušan D. and Marković, Zoran M. and Todorović Marković, Biljana M.", year = "2024", abstract = "In this study, nanochitosan dots (ChiDs) were synthesized using gamma rays and encapsulated in bacterial cellulose (BC) polymer matrix for antibiofilm potential in photodynamic therapy. The composites were analyzed for structural changes using SEM, AFM, FTIR, XRD, EPR, and porosity measurements. Additionally, ChiD release was assessed. The results showed that the chemical composition remained unaltered, but ChiD agglomerates embedded in BC changed shape (1.5–2.5 μm). Bacterial cellulose fibers became deformed and interconnected, with increased surface roughness and porosity and decreased crystallinity. No singlet oxygen formation was observed, and the total amount of released ChiD was up to 16.10%. Antibiofilm activity was higher under green light, with reductions ranging from 48 to 57% under blue light and 78 to 85% under green light. Methicillin-resistant Staphylococcus aureus was the most sensitive strain. The new photoactive composite hydrogels show promising potential for combating biofilm-related infections.", publisher = "Basel : MDPI", journal = "Journal of Functional Biomaterials", title = "Reduction in pathogenic biofilms by the photoactive composite of bacterial cellulose and nanochitosan dots under blue and green light", number = "3", pages = "72", volume = "15", doi = "10.3390/ jfb15030072" }
Zmejoski, D. Z., Zdravković, N. M., Budimir Filimonović, M. D., Pavlović, V. B., Butulija, S. V., Milivojević, D. D., Marković, Z. M.,& Todorović Marković, B. M.. (2024). Reduction in pathogenic biofilms by the photoactive composite of bacterial cellulose and nanochitosan dots under blue and green light. in Journal of Functional Biomaterials Basel : MDPI., 15(3), 72. https://doi.org/10.3390/ jfb15030072
Zmejoski DZ, Zdravković NM, Budimir Filimonović MD, Pavlović VB, Butulija SV, Milivojević DD, Marković ZM, Todorović Marković BM. Reduction in pathogenic biofilms by the photoactive composite of bacterial cellulose and nanochitosan dots under blue and green light. in Journal of Functional Biomaterials. 2024;15(3):72. doi:10.3390/ jfb15030072 .
Zmejoski, Danica Z., Zdravković, Nemanja M., Budimir Filimonović, Milica D., Pavlović, Vladimir B., Butulija, Svetlana V., Milivojević, Dušan D., Marković, Zoran M., Todorović Marković, Biljana M., "Reduction in pathogenic biofilms by the photoactive composite of bacterial cellulose and nanochitosan dots under blue and green light" in Journal of Functional Biomaterials, 15, no. 3 (2024):72, https://doi.org/10.3390/ jfb15030072 . .