Tick infestation and occurrence of Anaplasma phagocytophilum and piroplasms in cattle in the Republic of Serbia
2018
Преузимање 🢃
Аутори
Vasić, AnaMarion, Nieder
Zdravković, Nemanja
Bojkovski, Jovan
Bugarski, Dejan
Pavlović, Ivan
Silaghi, Cornelia
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Ticks transmit important pathogens affecting cattle such as intracellular bacteria of the genus Anaplasma or protozoa of the order
Piroplasmida. This study aimed at assessing tick species present on pastures and cattle and determining occurrence of the tickborne pathogens Anaplasma phagocytophilum and Babesia spp. in cattle. During May and June 2013, ticks and EDTA blood
were collected from 185 dairy cows at six locations in Serbia. Ticks were also collected directly from the pastures at four of these
locations. The occurrence of A. phagocytophilum was investigated by serological (immunofluorescence antibody test (IFAT)) and
molecular methods (real-time PCR) and of piroplasms by traditional PCR followed by sequencing. The most prevalent tick
species on animals was Ixodes ricinus, (n = 167), followed by Haemaphysalis punctata (n = 146) and Dermacentor marginatus
(n = 122). On the pasture, however, the most common species was H. punctata (n = 41), I. ricinus (n = 37), and D. margin...atus
(n = 2). Altogether, 4 out of 163 (2.45%) serum samples of cows were positive for A. phagocytophilum-specific antibodies by
IFAT. However, the 135 blood samples tested for A. phagocytophilum DNA were all negative. Altogether, 5 out of these 135
samples were positive for piroplasm DNA. PCR products were sequenced and identified as a benign Theileria spp. with 100%
identity with GenBank entries from Italy (Theileria sergenti), China (Theileria spp.), and Korea (Theileria buffeli isolate HS252).
The results provide evidence for the presence of several hard tick species infesting cattle in Serbia which can carry pathogens
potentially influencing animal health, as well as evidence of contact with tick-borne pathogens Theileria spp. and A.
phagocytophilum.
Кључне речи:
Dairy cows / Tick-borne pathogens / Ixodes ricinus / Dermacentor marginatus / Haemaphysalis punctata / SerbiaИзвор:
Parasitology Research, 2018, 117, 6, 1813-1818Издавач:
- New York : Springer
Финансирање / пројекти:
- Project was partially funded by the German Academic Exchange Service (DAAD) in the frame of the 2-year bilateral exchange project PPP Serbia 2013.
DOI: 10.1007/s00436-018-5867-x
ISSN: 0932-0113; 1432-1955 (electronic)
PubMed: 29679202
WoS: 000432128400016
Scopus: 2-s2.0-85045744388
URI
https://link.springer.com/article/10.1007/s00436-018-5867-x#citeashttps://reponivs.nivs.rs/handle/123456789/588
Институција/група
Naučni institut za veterinarstvo SrbijeTY - JOUR AU - Vasić, Ana AU - Marion, Nieder AU - Zdravković, Nemanja AU - Bojkovski, Jovan AU - Bugarski, Dejan AU - Pavlović, Ivan AU - Silaghi, Cornelia PY - 2018 UR - https://link.springer.com/article/10.1007/s00436-018-5867-x#citeas UR - https://reponivs.nivs.rs/handle/123456789/588 AB - Ticks transmit important pathogens affecting cattle such as intracellular bacteria of the genus Anaplasma or protozoa of the order Piroplasmida. This study aimed at assessing tick species present on pastures and cattle and determining occurrence of the tickborne pathogens Anaplasma phagocytophilum and Babesia spp. in cattle. During May and June 2013, ticks and EDTA blood were collected from 185 dairy cows at six locations in Serbia. Ticks were also collected directly from the pastures at four of these locations. The occurrence of A. phagocytophilum was investigated by serological (immunofluorescence antibody test (IFAT)) and molecular methods (real-time PCR) and of piroplasms by traditional PCR followed by sequencing. The most prevalent tick species on animals was Ixodes ricinus, (n = 167), followed by Haemaphysalis punctata (n = 146) and Dermacentor marginatus (n = 122). On the pasture, however, the most common species was H. punctata (n = 41), I. ricinus (n = 37), and D. marginatus (n = 2). Altogether, 4 out of 163 (2.45%) serum samples of cows were positive for A. phagocytophilum-specific antibodies by IFAT. However, the 135 blood samples tested for A. phagocytophilum DNA were all negative. Altogether, 5 out of these 135 samples were positive for piroplasm DNA. PCR products were sequenced and identified as a benign Theileria spp. with 100% identity with GenBank entries from Italy (Theileria sergenti), China (Theileria spp.), and Korea (Theileria buffeli isolate HS252). The results provide evidence for the presence of several hard tick species infesting cattle in Serbia which can carry pathogens potentially influencing animal health, as well as evidence of contact with tick-borne pathogens Theileria spp. and A. phagocytophilum. PB - New York : Springer T2 - Parasitology Research T1 - Tick infestation and occurrence of Anaplasma phagocytophilum and piroplasms in cattle in the Republic of Serbia EP - 1818 IS - 6 SP - 1813 VL - 117 VL - 1818 DO - 10.1007/s00436-018-5867-x ER -
@article{ author = "Vasić, Ana and Marion, Nieder and Zdravković, Nemanja and Bojkovski, Jovan and Bugarski, Dejan and Pavlović, Ivan and Silaghi, Cornelia", year = "2018", abstract = "Ticks transmit important pathogens affecting cattle such as intracellular bacteria of the genus Anaplasma or protozoa of the order Piroplasmida. This study aimed at assessing tick species present on pastures and cattle and determining occurrence of the tickborne pathogens Anaplasma phagocytophilum and Babesia spp. in cattle. During May and June 2013, ticks and EDTA blood were collected from 185 dairy cows at six locations in Serbia. Ticks were also collected directly from the pastures at four of these locations. The occurrence of A. phagocytophilum was investigated by serological (immunofluorescence antibody test (IFAT)) and molecular methods (real-time PCR) and of piroplasms by traditional PCR followed by sequencing. The most prevalent tick species on animals was Ixodes ricinus, (n = 167), followed by Haemaphysalis punctata (n = 146) and Dermacentor marginatus (n = 122). On the pasture, however, the most common species was H. punctata (n = 41), I. ricinus (n = 37), and D. marginatus (n = 2). Altogether, 4 out of 163 (2.45%) serum samples of cows were positive for A. phagocytophilum-specific antibodies by IFAT. However, the 135 blood samples tested for A. phagocytophilum DNA were all negative. Altogether, 5 out of these 135 samples were positive for piroplasm DNA. PCR products were sequenced and identified as a benign Theileria spp. with 100% identity with GenBank entries from Italy (Theileria sergenti), China (Theileria spp.), and Korea (Theileria buffeli isolate HS252). The results provide evidence for the presence of several hard tick species infesting cattle in Serbia which can carry pathogens potentially influencing animal health, as well as evidence of contact with tick-borne pathogens Theileria spp. and A. phagocytophilum.", publisher = "New York : Springer", journal = "Parasitology Research", title = "Tick infestation and occurrence of Anaplasma phagocytophilum and piroplasms in cattle in the Republic of Serbia", pages = "1818-1813", number = "6", volume = "117, 1818", doi = "10.1007/s00436-018-5867-x" }
Vasić, A., Marion, N., Zdravković, N., Bojkovski, J., Bugarski, D., Pavlović, I.,& Silaghi, C.. (2018). Tick infestation and occurrence of Anaplasma phagocytophilum and piroplasms in cattle in the Republic of Serbia. in Parasitology Research New York : Springer., 117(6), 1813-1818. https://doi.org/10.1007/s00436-018-5867-x
Vasić A, Marion N, Zdravković N, Bojkovski J, Bugarski D, Pavlović I, Silaghi C. Tick infestation and occurrence of Anaplasma phagocytophilum and piroplasms in cattle in the Republic of Serbia. in Parasitology Research. 2018;117(6):1813-1818. doi:10.1007/s00436-018-5867-x .
Vasić, Ana, Marion, Nieder, Zdravković, Nemanja, Bojkovski, Jovan, Bugarski, Dejan, Pavlović, Ivan, Silaghi, Cornelia, "Tick infestation and occurrence of Anaplasma phagocytophilum and piroplasms in cattle in the Republic of Serbia" in Parasitology Research, 117, no. 6 (2018):1813-1818, https://doi.org/10.1007/s00436-018-5867-x . .