Suitability of individual and bulk milk samples to investigate the humoral immune response to lumpy skin disease vaccination by ELISA
![](/themes/Miragereponivs//images/openAccess.png)
2020
Autori
Milovanović, Milovan![](/themes/Miragereponivs/images/orcid.png)
Milićević, Vesna
![](/themes/Miragereponivs/images/orcid.png)
Radojičić, Sonja
![](/themes/Miragereponivs/images/orcid.png)
Valčić, Miroslav
Hoffmann, Bernd
Dietze, Klaas
Članak u časopisu (Objavljena verzija)
BY, CC0
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Background The detection of antibodies against capripoxvirus has become easier with a commercially available ELISA validated for serum and plasma. In order to explore its suitability for immunological investigations on alternative samples, this study targeted milk as sample matrix available through non-invasive sampling. Methods Samples for this study were collected from dairy cows vaccinated against LSD in an area without reported LSD virus circulation. Paired serum and milk (individual and bulk) samples were tested by ELISA without and with modifications of the sample incubation time for the milk samples. For the evaluation of the test specificity, 352 milk samples from a milk repository in Germany were used as negative control. Receiver operating characteristic analysis was performed for determination of the Youden index and determination of the most suitable cut-off value for maximum specificity. Results From 154 analyzed serum samples from Serbia, 75 were detected as positive in t...he ELISA. Sensitivity and specificity of the ELISA test for milk samples reached values of 88 to 91% using Youden criteria. A cut-off of 10 was determined aiming for maximum specificity. This cut-off value was used for further analysis. Using the protocol for serum, out of 154 milk samples, 38 were detected as positive, number of positive detected milk samples increase up to 48 with modified protocol. Milk samples from Germany reacted negative, except two samples that had borderline results using modified protocol. Significant statistical difference (p LT 0.05) was observed between two incubation protocols. The detection of LSD-specific antibodies from bulk milk samples (pools of 2-10 individuals) came along with a reduced sensitivity over the sample of individual animals. Conclusions Results show that the detection of capripoxvirus specific antibodies in milk samples using the commercially available ELISA from IDvet is feasible and can represent a helpful tool for LSDV monitoring programs.
Ključne reči:
Surveillance / Serology / Non-invasive / Milk / Lumpy skin disease / Herd screening / ELISAIzvor:
Virology Journal, 2020, 17, 1Izdavač:
- BMC, London
Finansiranje / projekti:
- Razvoj i standardizacija molekularnih i imunskih metoda u dijagnostici bakterijskih zoonoza (RS-MESTD-Technological Development (TD or TR)-31088)
- Ekološka i virusološka istraživanja prisustva emerging zoonoza u rezervatima prirode Republike Srbije (RS-MESTD-Technological Development (TD or TR)-37015)
DOI: 10.1186/s12985-020-01298-x
ISSN: 1743-422X
PubMed: 32138740
WoS: 000519299400001
Scopus: 2-s2.0-85081530907
Institucija/grupa
Naučni institut za veterinarstvo SrbijeTY - JOUR AU - Milovanović, Milovan AU - Milićević, Vesna AU - Radojičić, Sonja AU - Valčić, Miroslav AU - Hoffmann, Bernd AU - Dietze, Klaas PY - 2020 UR - https://reponivs.nivs.rs/handle/123456789/422 AB - Background The detection of antibodies against capripoxvirus has become easier with a commercially available ELISA validated for serum and plasma. In order to explore its suitability for immunological investigations on alternative samples, this study targeted milk as sample matrix available through non-invasive sampling. Methods Samples for this study were collected from dairy cows vaccinated against LSD in an area without reported LSD virus circulation. Paired serum and milk (individual and bulk) samples were tested by ELISA without and with modifications of the sample incubation time for the milk samples. For the evaluation of the test specificity, 352 milk samples from a milk repository in Germany were used as negative control. Receiver operating characteristic analysis was performed for determination of the Youden index and determination of the most suitable cut-off value for maximum specificity. Results From 154 analyzed serum samples from Serbia, 75 were detected as positive in the ELISA. Sensitivity and specificity of the ELISA test for milk samples reached values of 88 to 91% using Youden criteria. A cut-off of 10 was determined aiming for maximum specificity. This cut-off value was used for further analysis. Using the protocol for serum, out of 154 milk samples, 38 were detected as positive, number of positive detected milk samples increase up to 48 with modified protocol. Milk samples from Germany reacted negative, except two samples that had borderline results using modified protocol. Significant statistical difference (p LT 0.05) was observed between two incubation protocols. The detection of LSD-specific antibodies from bulk milk samples (pools of 2-10 individuals) came along with a reduced sensitivity over the sample of individual animals. Conclusions Results show that the detection of capripoxvirus specific antibodies in milk samples using the commercially available ELISA from IDvet is feasible and can represent a helpful tool for LSDV monitoring programs. PB - BMC, London T2 - Virology Journal T1 - Suitability of individual and bulk milk samples to investigate the humoral immune response to lumpy skin disease vaccination by ELISA IS - 1 VL - 17 DO - 10.1186/s12985-020-01298-x UR - conv_543 ER -
@article{ author = "Milovanović, Milovan and Milićević, Vesna and Radojičić, Sonja and Valčić, Miroslav and Hoffmann, Bernd and Dietze, Klaas", year = "2020", abstract = "Background The detection of antibodies against capripoxvirus has become easier with a commercially available ELISA validated for serum and plasma. In order to explore its suitability for immunological investigations on alternative samples, this study targeted milk as sample matrix available through non-invasive sampling. Methods Samples for this study were collected from dairy cows vaccinated against LSD in an area without reported LSD virus circulation. Paired serum and milk (individual and bulk) samples were tested by ELISA without and with modifications of the sample incubation time for the milk samples. For the evaluation of the test specificity, 352 milk samples from a milk repository in Germany were used as negative control. Receiver operating characteristic analysis was performed for determination of the Youden index and determination of the most suitable cut-off value for maximum specificity. Results From 154 analyzed serum samples from Serbia, 75 were detected as positive in the ELISA. Sensitivity and specificity of the ELISA test for milk samples reached values of 88 to 91% using Youden criteria. A cut-off of 10 was determined aiming for maximum specificity. This cut-off value was used for further analysis. Using the protocol for serum, out of 154 milk samples, 38 were detected as positive, number of positive detected milk samples increase up to 48 with modified protocol. Milk samples from Germany reacted negative, except two samples that had borderline results using modified protocol. Significant statistical difference (p LT 0.05) was observed between two incubation protocols. The detection of LSD-specific antibodies from bulk milk samples (pools of 2-10 individuals) came along with a reduced sensitivity over the sample of individual animals. Conclusions Results show that the detection of capripoxvirus specific antibodies in milk samples using the commercially available ELISA from IDvet is feasible and can represent a helpful tool for LSDV monitoring programs.", publisher = "BMC, London", journal = "Virology Journal", title = "Suitability of individual and bulk milk samples to investigate the humoral immune response to lumpy skin disease vaccination by ELISA", number = "1", volume = "17", doi = "10.1186/s12985-020-01298-x", url = "conv_543" }
Milovanović, M., Milićević, V., Radojičić, S., Valčić, M., Hoffmann, B.,& Dietze, K.. (2020). Suitability of individual and bulk milk samples to investigate the humoral immune response to lumpy skin disease vaccination by ELISA. in Virology Journal BMC, London., 17(1). https://doi.org/10.1186/s12985-020-01298-x conv_543
Milovanović M, Milićević V, Radojičić S, Valčić M, Hoffmann B, Dietze K. Suitability of individual and bulk milk samples to investigate the humoral immune response to lumpy skin disease vaccination by ELISA. in Virology Journal. 2020;17(1). doi:10.1186/s12985-020-01298-x conv_543 .
Milovanović, Milovan, Milićević, Vesna, Radojičić, Sonja, Valčić, Miroslav, Hoffmann, Bernd, Dietze, Klaas, "Suitability of individual and bulk milk samples to investigate the humoral immune response to lumpy skin disease vaccination by ELISA" in Virology Journal, 17, no. 1 (2020), https://doi.org/10.1186/s12985-020-01298-x ., conv_543 .