Evidence of West Nile Virus (WNV) Circulation in Wild Birds and WNV RNA Negativity in Mosquitoes of the Danube Delta Biosphere Reserve, Romania, 2016
2019
Preuzimanje 🢃
Autori
Vasić, AnaOslobanu, Elena Luanda
Marinov, Mihai
Crivei, Luciana
Ratoi, Ioana Alexandra
Anita, Adriana
Anita, Dragos
Dorosencu, Alexandru
Alexe, Vasile
Raileanu, Stefan
Simeunović, Predrag
Raileanu, Cristian
Falcuta, Elena
Prioteasa, Florian Liviu
Bojkovski, Jovan
Pavlović, Ivan
Mathis, Alexander
Tews, Birke Andrea
Savuta, Gheorghe
Veronesi, Eva
Silaghi, Cornelia
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
West Nile virus (WNV) is a zoonotic flavivirus whose transmission cycle in nature includes wild birds as amplifying hosts and ornithophilic mosquito vectors. Bridge vectors can transmit WNV to mammal species potentially causing West Nile Fever. Wild bird migration is a mode of WNV introduction into new areas. The Danube Delta Biosphere Reserve (DDBR) is a major stopover of wild birds migrating between Europe and Africa. The aim of this study was to investigate the presence of WNV in the DDBR during the 2016 transmission season in wild birds and mosquitoes. Blood from 68 wild birds (nine different species) trapped at four different locations was analyzed by competitive ELISA and Virus Neutralization Test (VNT), revealing positive results in 8/68 (11.8%) of the wild birds by ELISA of which six samples (three from juvenile birds) were confirmed seropositive by VNT. Mosquitoes (n = 6523, 5 genera) were trapped with CDC Mini Light traps at two locations and in one location resting mosquitoe...s were caught. The presence of WNV RNA was tested in 134 pools by reverse transcription quantitative PCR (RT-qPCR). None of the pools was positive for WNV-specific RNA. Based on the obtained results, WNV was circulating in the DDBR during 2016.
Ključne reči:
wild birds / West Nile virus / VNT / ELISA / Danube Delta / Anopheles spp. / Aedes spp.Izvor:
Tropical Medicine and Infectious Disease, 2019, 4, 3Izdavač:
- MDPI, Basel
Finansiranje / projekti:
- SCOPES program of the Swiss National Science Foundation (SNFS)
- Swiss Agency for Development and Cooperation (SDC)
- Bundesamt fur Lebensmittelsicherheit und Veterinarwesen (Switzerland)
DOI: 10.3390/tropicalmed4030116
ISSN: 2414-6366
PubMed: 31438608
WoS: 000656483700020
Scopus: 2-s2.0-85072114990
Institucija/grupa
Naučni institut za veterinarstvo SrbijeTY - JOUR AU - Vasić, Ana AU - Oslobanu, Elena Luanda AU - Marinov, Mihai AU - Crivei, Luciana AU - Ratoi, Ioana Alexandra AU - Anita, Adriana AU - Anita, Dragos AU - Dorosencu, Alexandru AU - Alexe, Vasile AU - Raileanu, Stefan AU - Simeunović, Predrag AU - Raileanu, Cristian AU - Falcuta, Elena AU - Prioteasa, Florian Liviu AU - Bojkovski, Jovan AU - Pavlović, Ivan AU - Mathis, Alexander AU - Tews, Birke Andrea AU - Savuta, Gheorghe AU - Veronesi, Eva AU - Silaghi, Cornelia PY - 2019 UR - https://reponivs.nivs.rs/handle/123456789/384 AB - West Nile virus (WNV) is a zoonotic flavivirus whose transmission cycle in nature includes wild birds as amplifying hosts and ornithophilic mosquito vectors. Bridge vectors can transmit WNV to mammal species potentially causing West Nile Fever. Wild bird migration is a mode of WNV introduction into new areas. The Danube Delta Biosphere Reserve (DDBR) is a major stopover of wild birds migrating between Europe and Africa. The aim of this study was to investigate the presence of WNV in the DDBR during the 2016 transmission season in wild birds and mosquitoes. Blood from 68 wild birds (nine different species) trapped at four different locations was analyzed by competitive ELISA and Virus Neutralization Test (VNT), revealing positive results in 8/68 (11.8%) of the wild birds by ELISA of which six samples (three from juvenile birds) were confirmed seropositive by VNT. Mosquitoes (n = 6523, 5 genera) were trapped with CDC Mini Light traps at two locations and in one location resting mosquitoes were caught. The presence of WNV RNA was tested in 134 pools by reverse transcription quantitative PCR (RT-qPCR). None of the pools was positive for WNV-specific RNA. Based on the obtained results, WNV was circulating in the DDBR during 2016. PB - MDPI, Basel T2 - Tropical Medicine and Infectious Disease T1 - Evidence of West Nile Virus (WNV) Circulation in Wild Birds and WNV RNA Negativity in Mosquitoes of the Danube Delta Biosphere Reserve, Romania, 2016 IS - 3 VL - 4 DO - 10.3390/tropicalmed4030116 UR - conv_566 ER -
@article{ author = "Vasić, Ana and Oslobanu, Elena Luanda and Marinov, Mihai and Crivei, Luciana and Ratoi, Ioana Alexandra and Anita, Adriana and Anita, Dragos and Dorosencu, Alexandru and Alexe, Vasile and Raileanu, Stefan and Simeunović, Predrag and Raileanu, Cristian and Falcuta, Elena and Prioteasa, Florian Liviu and Bojkovski, Jovan and Pavlović, Ivan and Mathis, Alexander and Tews, Birke Andrea and Savuta, Gheorghe and Veronesi, Eva and Silaghi, Cornelia", year = "2019", abstract = "West Nile virus (WNV) is a zoonotic flavivirus whose transmission cycle in nature includes wild birds as amplifying hosts and ornithophilic mosquito vectors. Bridge vectors can transmit WNV to mammal species potentially causing West Nile Fever. Wild bird migration is a mode of WNV introduction into new areas. The Danube Delta Biosphere Reserve (DDBR) is a major stopover of wild birds migrating between Europe and Africa. The aim of this study was to investigate the presence of WNV in the DDBR during the 2016 transmission season in wild birds and mosquitoes. Blood from 68 wild birds (nine different species) trapped at four different locations was analyzed by competitive ELISA and Virus Neutralization Test (VNT), revealing positive results in 8/68 (11.8%) of the wild birds by ELISA of which six samples (three from juvenile birds) were confirmed seropositive by VNT. Mosquitoes (n = 6523, 5 genera) were trapped with CDC Mini Light traps at two locations and in one location resting mosquitoes were caught. The presence of WNV RNA was tested in 134 pools by reverse transcription quantitative PCR (RT-qPCR). None of the pools was positive for WNV-specific RNA. Based on the obtained results, WNV was circulating in the DDBR during 2016.", publisher = "MDPI, Basel", journal = "Tropical Medicine and Infectious Disease", title = "Evidence of West Nile Virus (WNV) Circulation in Wild Birds and WNV RNA Negativity in Mosquitoes of the Danube Delta Biosphere Reserve, Romania, 2016", number = "3", volume = "4", doi = "10.3390/tropicalmed4030116", url = "conv_566" }
Vasić, A., Oslobanu, E. L., Marinov, M., Crivei, L., Ratoi, I. A., Anita, A., Anita, D., Dorosencu, A., Alexe, V., Raileanu, S., Simeunović, P., Raileanu, C., Falcuta, E., Prioteasa, F. L., Bojkovski, J., Pavlović, I., Mathis, A., Tews, B. A., Savuta, G., Veronesi, E.,& Silaghi, C.. (2019). Evidence of West Nile Virus (WNV) Circulation in Wild Birds and WNV RNA Negativity in Mosquitoes of the Danube Delta Biosphere Reserve, Romania, 2016. in Tropical Medicine and Infectious Disease MDPI, Basel., 4(3). https://doi.org/10.3390/tropicalmed4030116 conv_566
Vasić A, Oslobanu EL, Marinov M, Crivei L, Ratoi IA, Anita A, Anita D, Dorosencu A, Alexe V, Raileanu S, Simeunović P, Raileanu C, Falcuta E, Prioteasa FL, Bojkovski J, Pavlović I, Mathis A, Tews BA, Savuta G, Veronesi E, Silaghi C. Evidence of West Nile Virus (WNV) Circulation in Wild Birds and WNV RNA Negativity in Mosquitoes of the Danube Delta Biosphere Reserve, Romania, 2016. in Tropical Medicine and Infectious Disease. 2019;4(3). doi:10.3390/tropicalmed4030116 conv_566 .
Vasić, Ana, Oslobanu, Elena Luanda, Marinov, Mihai, Crivei, Luciana, Ratoi, Ioana Alexandra, Anita, Adriana, Anita, Dragos, Dorosencu, Alexandru, Alexe, Vasile, Raileanu, Stefan, Simeunović, Predrag, Raileanu, Cristian, Falcuta, Elena, Prioteasa, Florian Liviu, Bojkovski, Jovan, Pavlović, Ivan, Mathis, Alexander, Tews, Birke Andrea, Savuta, Gheorghe, Veronesi, Eva, Silaghi, Cornelia, "Evidence of West Nile Virus (WNV) Circulation in Wild Birds and WNV RNA Negativity in Mosquitoes of the Danube Delta Biosphere Reserve, Romania, 2016" in Tropical Medicine and Infectious Disease, 4, no. 3 (2019), https://doi.org/10.3390/tropicalmed4030116 ., conv_566 .