Multilocus sequence typing of canine Giardia duodenalis from South Eastern European countries
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2015
Authors
Sommer, M.F.Beck, R.
Ionita, M.
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Stefanovska, J.
Vasić, Ana
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Zdravković, Nemanja
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Hamel, D.
Rehbein, S.
Knaus, M.
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Mitrea, I. L.
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Shukullari, E.
Kirkova, Z.
Rapti, D.
Capari, B.
Silaghi, Cornelia
Article (Published version)
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Show full item recordAbstract
Giardia duodenalis is a worldwide occurring protozoan that can infect various mammalian hosts. While living conditions are getting closer between pet animals and owners, there is discussion whether dogs may contribute to the transmission of these pathogens to humans. The present study was conducted in order to identify the Giardia assemblages in dogs from South Eastern Europe. For this purpose, 1645 faecal samples of household and shelter dogs from Albania, Bulgaria, Hungary, Macedonia, Romania and Serbia were tested for Giardia coproantigen by enzyme-linked immunosorbent assay (ELISA). A subset of 107 faecal samples demonstrating Giardia cysts by direct immunofluorescence assay (IFA) or microscopy (15-22 per country) plus 26 IFA-positive canine faecal samples from Croatia were used for DNA extraction and multilocus sequence typing with nested PCRs targeting five different gene loci: SSU rRNA, ITS1-5.8S-ITS2, beta giardin (bg), glutamate dehydrogenase (gdh) and triosephosphate isomeras...e (tpi). One third (33.7 %) of the samples tested positive for Giardia antigen in the coproantigen ELISA. Shelter dogs were infected more frequently than household dogs (57.2 vs. 29.7 %, p LT 0.01). Amplification was obtained in 82.0, 12.8, 11.3, 1.5, and 31.6 %, of the investigated samples at the SSU rRNA, bg, gdh and tpi loci and the ITS1-5.8S-ITS2 region, respectively. The dog-specific assemblages C and D were identified in 50 and 68 samples, respectively. The results demonstrate that G. duodenalis should be considered as a common parasite in dogs from South Eastern Europe. However, there was no evidence for zoonotic Giardia assemblages in the investigated canine subpopulation.
Keywords:
South Eastern Europe / Multilocus genotyping / Giardia duodenalis / Dog / AssemblagesSource:
Parasitology Research, 2015, 114, 6, 2165-2174Publisher:
- Springer, New York
Funding / projects:
- 'Bayerisches Hochschulzentrum fur Mittel-, Ost- und Sudosteuropa' (BAYHOST)
DOI: 10.1007/s00436-015-4405-3
ISSN: 0932-0113
PubMed: 25804971
WoS: 000354404100015
Scopus: 2-s2.0-84940008609
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Institution/Community
Naučni institut za veterinarstvo SrbijeTY - JOUR AU - Sommer, M.F. AU - Beck, R. AU - Ionita, M. AU - Stefanovska, J. AU - Vasić, Ana AU - Zdravković, Nemanja AU - Hamel, D. AU - Rehbein, S. AU - Knaus, M. AU - Mitrea, I. L. AU - Shukullari, E. AU - Kirkova, Z. AU - Rapti, D. AU - Capari, B. AU - Silaghi, Cornelia PY - 2015 UR - https://reponivs.nivs.rs/handle/123456789/303 AB - Giardia duodenalis is a worldwide occurring protozoan that can infect various mammalian hosts. While living conditions are getting closer between pet animals and owners, there is discussion whether dogs may contribute to the transmission of these pathogens to humans. The present study was conducted in order to identify the Giardia assemblages in dogs from South Eastern Europe. For this purpose, 1645 faecal samples of household and shelter dogs from Albania, Bulgaria, Hungary, Macedonia, Romania and Serbia were tested for Giardia coproantigen by enzyme-linked immunosorbent assay (ELISA). A subset of 107 faecal samples demonstrating Giardia cysts by direct immunofluorescence assay (IFA) or microscopy (15-22 per country) plus 26 IFA-positive canine faecal samples from Croatia were used for DNA extraction and multilocus sequence typing with nested PCRs targeting five different gene loci: SSU rRNA, ITS1-5.8S-ITS2, beta giardin (bg), glutamate dehydrogenase (gdh) and triosephosphate isomerase (tpi). One third (33.7 %) of the samples tested positive for Giardia antigen in the coproantigen ELISA. Shelter dogs were infected more frequently than household dogs (57.2 vs. 29.7 %, p LT 0.01). Amplification was obtained in 82.0, 12.8, 11.3, 1.5, and 31.6 %, of the investigated samples at the SSU rRNA, bg, gdh and tpi loci and the ITS1-5.8S-ITS2 region, respectively. The dog-specific assemblages C and D were identified in 50 and 68 samples, respectively. The results demonstrate that G. duodenalis should be considered as a common parasite in dogs from South Eastern Europe. However, there was no evidence for zoonotic Giardia assemblages in the investigated canine subpopulation. PB - Springer, New York T2 - Parasitology Research T1 - Multilocus sequence typing of canine Giardia duodenalis from South Eastern European countries EP - 2174 IS - 6 SP - 2165 VL - 114 DO - 10.1007/s00436-015-4405-3 UR - conv_450 ER -
@article{ author = "Sommer, M.F. and Beck, R. and Ionita, M. and Stefanovska, J. and Vasić, Ana and Zdravković, Nemanja and Hamel, D. and Rehbein, S. and Knaus, M. and Mitrea, I. L. and Shukullari, E. and Kirkova, Z. and Rapti, D. and Capari, B. and Silaghi, Cornelia", year = "2015", abstract = "Giardia duodenalis is a worldwide occurring protozoan that can infect various mammalian hosts. While living conditions are getting closer between pet animals and owners, there is discussion whether dogs may contribute to the transmission of these pathogens to humans. The present study was conducted in order to identify the Giardia assemblages in dogs from South Eastern Europe. For this purpose, 1645 faecal samples of household and shelter dogs from Albania, Bulgaria, Hungary, Macedonia, Romania and Serbia were tested for Giardia coproantigen by enzyme-linked immunosorbent assay (ELISA). A subset of 107 faecal samples demonstrating Giardia cysts by direct immunofluorescence assay (IFA) or microscopy (15-22 per country) plus 26 IFA-positive canine faecal samples from Croatia were used for DNA extraction and multilocus sequence typing with nested PCRs targeting five different gene loci: SSU rRNA, ITS1-5.8S-ITS2, beta giardin (bg), glutamate dehydrogenase (gdh) and triosephosphate isomerase (tpi). One third (33.7 %) of the samples tested positive for Giardia antigen in the coproantigen ELISA. Shelter dogs were infected more frequently than household dogs (57.2 vs. 29.7 %, p LT 0.01). Amplification was obtained in 82.0, 12.8, 11.3, 1.5, and 31.6 %, of the investigated samples at the SSU rRNA, bg, gdh and tpi loci and the ITS1-5.8S-ITS2 region, respectively. The dog-specific assemblages C and D were identified in 50 and 68 samples, respectively. The results demonstrate that G. duodenalis should be considered as a common parasite in dogs from South Eastern Europe. However, there was no evidence for zoonotic Giardia assemblages in the investigated canine subpopulation.", publisher = "Springer, New York", journal = "Parasitology Research", title = "Multilocus sequence typing of canine Giardia duodenalis from South Eastern European countries", pages = "2174-2165", number = "6", volume = "114", doi = "10.1007/s00436-015-4405-3", url = "conv_450" }
Sommer, M.F., Beck, R., Ionita, M., Stefanovska, J., Vasić, A., Zdravković, N., Hamel, D., Rehbein, S., Knaus, M., Mitrea, I. L., Shukullari, E., Kirkova, Z., Rapti, D., Capari, B.,& Silaghi, C.. (2015). Multilocus sequence typing of canine Giardia duodenalis from South Eastern European countries. in Parasitology Research Springer, New York., 114(6), 2165-2174. https://doi.org/10.1007/s00436-015-4405-3 conv_450
Sommer M, Beck R, Ionita M, Stefanovska J, Vasić A, Zdravković N, Hamel D, Rehbein S, Knaus M, Mitrea IL, Shukullari E, Kirkova Z, Rapti D, Capari B, Silaghi C. Multilocus sequence typing of canine Giardia duodenalis from South Eastern European countries. in Parasitology Research. 2015;114(6):2165-2174. doi:10.1007/s00436-015-4405-3 conv_450 .
Sommer, M.F., Beck, R., Ionita, M., Stefanovska, J., Vasić, Ana, Zdravković, Nemanja, Hamel, D., Rehbein, S., Knaus, M., Mitrea, I. L., Shukullari, E., Kirkova, Z., Rapti, D., Capari, B., Silaghi, Cornelia, "Multilocus sequence typing of canine Giardia duodenalis from South Eastern European countries" in Parasitology Research, 114, no. 6 (2015):2165-2174, https://doi.org/10.1007/s00436-015-4405-3 ., conv_450 .