Genetic characterization and phylogenetic analysis of porcine circovirus type 2 (PCV2) in Serbia
Само за регистроване кориснике
2012
Аутори
Savić, BožidarMilićević, Vesna
Jakić-Dimić, Dobrila
Bojkovski, Jovan
Prodanović, Radiša
Kureljušić, Branislav
Potkonjak, Aleksandar
Savić, Borivoje
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Porcine circovirus type 2 (PCV2) is the main causative agent of postweaning multisystemic wasting syndrome (PMWS). To characterize and determine the genetic diversity of PCV2 in the porcine population of Serbia, nucleotide and deduced amino acid sequences of the open reading frame 2 (ORF2) of PCV2 collected from the tissues of pigs that either had died as a result of PMWS or did not exhibit disease symptoms were analyzed. Sequencing and phylogenetic analysis showed considerable diversity among PCV2 ORF2 sequences and the existence of two main PCV2 genotypes, PCV2b and PCV2a, with at least three clusters, 1A/B, 1C and 2D. In order to provide further proof that the 1C strain is circulating in the porcine population, the whole viral genome of one PCV2 isolate was sequenced. Genotyping and phylogenetic analysis using the entire viral genome sequences confirmed that there was a PMWS-associated 1C strain emerging in Serbia. Our analysis also showed that PCV2b is dominant in the porcine popul...ation, and that it is exclusively associated with PMWS occurrences in the country. These data constitute a useful basis for further epidemiological studies regarding the heterogeneity of PCV2 strains on the European continent.
Извор:
Archives of Virology, 2012, 157, 1, 21-28Издавач:
- Springer Wien, Wien
Финансирање / пројекти:
- Унапређење здравља и добробити високопродуктивних крава идентификацијом и отклањањем стресогених фактора (RS-MESTD-Technological Development (TD or TR)-31062)
DOI: 10.1007/s00705-011-1130-9
ISSN: 0304-8608
PubMed: 21964944
WoS: 000299845800002
Scopus: 2-s2.0-84855312239
Институција/група
Naučni institut za veterinarstvo SrbijeTY - JOUR AU - Savić, Božidar AU - Milićević, Vesna AU - Jakić-Dimić, Dobrila AU - Bojkovski, Jovan AU - Prodanović, Radiša AU - Kureljušić, Branislav AU - Potkonjak, Aleksandar AU - Savić, Borivoje PY - 2012 UR - https://reponivs.nivs.rs/handle/123456789/186 AB - Porcine circovirus type 2 (PCV2) is the main causative agent of postweaning multisystemic wasting syndrome (PMWS). To characterize and determine the genetic diversity of PCV2 in the porcine population of Serbia, nucleotide and deduced amino acid sequences of the open reading frame 2 (ORF2) of PCV2 collected from the tissues of pigs that either had died as a result of PMWS or did not exhibit disease symptoms were analyzed. Sequencing and phylogenetic analysis showed considerable diversity among PCV2 ORF2 sequences and the existence of two main PCV2 genotypes, PCV2b and PCV2a, with at least three clusters, 1A/B, 1C and 2D. In order to provide further proof that the 1C strain is circulating in the porcine population, the whole viral genome of one PCV2 isolate was sequenced. Genotyping and phylogenetic analysis using the entire viral genome sequences confirmed that there was a PMWS-associated 1C strain emerging in Serbia. Our analysis also showed that PCV2b is dominant in the porcine population, and that it is exclusively associated with PMWS occurrences in the country. These data constitute a useful basis for further epidemiological studies regarding the heterogeneity of PCV2 strains on the European continent. PB - Springer Wien, Wien T2 - Archives of Virology T1 - Genetic characterization and phylogenetic analysis of porcine circovirus type 2 (PCV2) in Serbia EP - 28 IS - 1 SP - 21 VL - 157 DO - 10.1007/s00705-011-1130-9 UR - conv_389 ER -
@article{ author = "Savić, Božidar and Milićević, Vesna and Jakić-Dimić, Dobrila and Bojkovski, Jovan and Prodanović, Radiša and Kureljušić, Branislav and Potkonjak, Aleksandar and Savić, Borivoje", year = "2012", abstract = "Porcine circovirus type 2 (PCV2) is the main causative agent of postweaning multisystemic wasting syndrome (PMWS). To characterize and determine the genetic diversity of PCV2 in the porcine population of Serbia, nucleotide and deduced amino acid sequences of the open reading frame 2 (ORF2) of PCV2 collected from the tissues of pigs that either had died as a result of PMWS or did not exhibit disease symptoms were analyzed. Sequencing and phylogenetic analysis showed considerable diversity among PCV2 ORF2 sequences and the existence of two main PCV2 genotypes, PCV2b and PCV2a, with at least three clusters, 1A/B, 1C and 2D. In order to provide further proof that the 1C strain is circulating in the porcine population, the whole viral genome of one PCV2 isolate was sequenced. Genotyping and phylogenetic analysis using the entire viral genome sequences confirmed that there was a PMWS-associated 1C strain emerging in Serbia. Our analysis also showed that PCV2b is dominant in the porcine population, and that it is exclusively associated with PMWS occurrences in the country. These data constitute a useful basis for further epidemiological studies regarding the heterogeneity of PCV2 strains on the European continent.", publisher = "Springer Wien, Wien", journal = "Archives of Virology", title = "Genetic characterization and phylogenetic analysis of porcine circovirus type 2 (PCV2) in Serbia", pages = "28-21", number = "1", volume = "157", doi = "10.1007/s00705-011-1130-9", url = "conv_389" }
Savić, B., Milićević, V., Jakić-Dimić, D., Bojkovski, J., Prodanović, R., Kureljušić, B., Potkonjak, A.,& Savić, B.. (2012). Genetic characterization and phylogenetic analysis of porcine circovirus type 2 (PCV2) in Serbia. in Archives of Virology Springer Wien, Wien., 157(1), 21-28. https://doi.org/10.1007/s00705-011-1130-9 conv_389
Savić B, Milićević V, Jakić-Dimić D, Bojkovski J, Prodanović R, Kureljušić B, Potkonjak A, Savić B. Genetic characterization and phylogenetic analysis of porcine circovirus type 2 (PCV2) in Serbia. in Archives of Virology. 2012;157(1):21-28. doi:10.1007/s00705-011-1130-9 conv_389 .
Savić, Božidar, Milićević, Vesna, Jakić-Dimić, Dobrila, Bojkovski, Jovan, Prodanović, Radiša, Kureljušić, Branislav, Potkonjak, Aleksandar, Savić, Borivoje, "Genetic characterization and phylogenetic analysis of porcine circovirus type 2 (PCV2) in Serbia" in Archives of Virology, 157, no. 1 (2012):21-28, https://doi.org/10.1007/s00705-011-1130-9 ., conv_389 .