TY  - CONF
AU  - Ninković, Milan
AU  - Žutić, Jadranka
AU  - Maksimović Zorić, Jelena
AU  - Jezdimirović, Nemanja
AU  - Milićević, Vesna
PY  - 2024-04-10
UR  - https://reponivs.nivs.rs/handle/123456789/974
AB  - Слинавка и шап (СиШ) спада у нарочито опасне заразне болести  домаћих папкара  и карактерише је веома нагло и брзо ширење. Болест се преноси директним и индиректним контактом. Вирус је јако отпоран у окружењу где је присутна болест и унутар исте фарме се може лако проширити путем различитих вектора, опреме и чак ветра. Циљ овог  рада је прикаже принципе и процедуре санитарних мера на газдинству у случају појаве Слинавке и Шапа. Важан сегмент сузбијања заразе јесте и дезинфекција зараженог газдинства, објеката, опреме, возила, отпадног и другог контаминираног материјала. Поштовање процедура чишћења, прања, дезинфекције су од виталног значаја за искорењивање СиШ на газдинству. Након уклањања лешева врши се прелиминарна дезинфекција газдинства. После 24 сата започиње се просес текућег чишћења и дезинфекције по завршетку дезинфекције, врши се одмор објекта у трајању од 7 дана при чему се врши завршно чишћење и дезинфекција објеката. Поновно усељевање животиња у објекте  могуће након укидања мера у зараженом и угроженој зони. Мере и начин ближег поступања на газдинству у случају су дефинисане правилником о утврђивању мера за рано откривање, дијагностику, спречавање ширења, сузбијање и искорењивање заразне болести Слинавке и Шап, "Службени гласник Републике Србије, 15/10".
AB  - Foot-and-mouth disease (FMD) is one of the particularly dangerous infectious diseases of domestic ungulates and is characterized by a very sudden and rapid spread. The disease is transmitted by direct and indirect contact. The virus is very resistant in the environment where the disease is present and within the same farm it can easily spread through different vectors, equipment and even wind. The aim of this work is to present the principles and procedures of sanitary measures on the farm in case of the occurrence of FMD. Disinfection of infected farms, facilities, equipment, vehicles, waste and other contaminated material is an important segment of infection control. Observance of cleaning, washing, disinfection procedures are of vital importance for the eradication of MFD on the farm. After removing the corpses, preliminary disinfection of the farm is carried out. After 24 hours, the process of ongoing cleaning and disinfection begins, after 7 days of rest, the final cleaning and disinfection of the facilities is carried out. Repopulation of animals into facilities is carried out after the lifting of measures in the infected and protective zone. The measures and method of closer treatment on the farm in the case are defined by the rulebook on establishing measures for early detection, diagnosis, prevention of spread, suppression and eradication of the infectious disease FMD, "Official Gazette of the Republic of Serbia, 15/10".
PB  - Beograd : Fakultet veterinarske medicine, Sekcija za zoonoze
C3  - XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
T1  - Примена санитарних мера на газдинству у случају појаве слинавке и шапа
T1  - Implementation of sanitary measures on the farm in the case of Foot and mouth disease
EP  - 111
SP  - 110
ER  - 

@conference{
author = "Ninković, Milan and Žutić, Jadranka and Maksimović Zorić, Jelena and Jezdimirović, Nemanja and Milićević, Vesna",
year = "2024-04-10",
abstract = "Слинавка и шап (СиШ) спада у нарочито опасне заразне болести  домаћих папкара  и карактерише је веома нагло и брзо ширење. Болест се преноси директним и индиректним контактом. Вирус је јако отпоран у окружењу где је присутна болест и унутар исте фарме се може лако проширити путем различитих вектора, опреме и чак ветра. Циљ овог  рада је прикаже принципе и процедуре санитарних мера на газдинству у случају појаве Слинавке и Шапа. Важан сегмент сузбијања заразе јесте и дезинфекција зараженог газдинства, објеката, опреме, возила, отпадног и другог контаминираног материјала. Поштовање процедура чишћења, прања, дезинфекције су од виталног значаја за искорењивање СиШ на газдинству. Након уклањања лешева врши се прелиминарна дезинфекција газдинства. После 24 сата започиње се просес текућег чишћења и дезинфекције по завршетку дезинфекције, врши се одмор објекта у трајању од 7 дана при чему се врши завршно чишћење и дезинфекција објеката. Поновно усељевање животиња у објекте  могуће након укидања мера у зараженом и угроженој зони. Мере и начин ближег поступања на газдинству у случају су дефинисане правилником о утврђивању мера за рано откривање, дијагностику, спречавање ширења, сузбијање и искорењивање заразне болести Слинавке и Шап, "Службени гласник Републике Србије, 15/10"., Foot-and-mouth disease (FMD) is one of the particularly dangerous infectious diseases of domestic ungulates and is characterized by a very sudden and rapid spread. The disease is transmitted by direct and indirect contact. The virus is very resistant in the environment where the disease is present and within the same farm it can easily spread through different vectors, equipment and even wind. The aim of this work is to present the principles and procedures of sanitary measures on the farm in case of the occurrence of FMD. Disinfection of infected farms, facilities, equipment, vehicles, waste and other contaminated material is an important segment of infection control. Observance of cleaning, washing, disinfection procedures are of vital importance for the eradication of MFD on the farm. After removing the corpses, preliminary disinfection of the farm is carried out. After 24 hours, the process of ongoing cleaning and disinfection begins, after 7 days of rest, the final cleaning and disinfection of the facilities is carried out. Repopulation of animals into facilities is carried out after the lifting of measures in the infected and protective zone. The measures and method of closer treatment on the farm in the case are defined by the rulebook on establishing measures for early detection, diagnosis, prevention of spread, suppression and eradication of the infectious disease FMD, "Official Gazette of the Republic of Serbia, 15/10".",
publisher = "Beograd : Fakultet veterinarske medicine, Sekcija za zoonoze",
journal = "XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.",
title = "Примена санитарних мера на газдинству у случају појаве слинавке и шапа, Implementation of sanitary measures on the farm in the case of Foot and mouth disease",
pages = "111-110"
}

Ninković, M., Žutić, J., Maksimović Zorić, J., Jezdimirović, N.,& Milićević, V.. (2024-04-10). Примена санитарних мера на газдинству у случају појаве слинавке и шапа. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
Beograd : Fakultet veterinarske medicine, Sekcija za zoonoze., 110-111.

Ninković M, Žutić J, Maksimović Zorić J, Jezdimirović N, Milićević V. Примена санитарних мера на газдинству у случају појаве слинавке и шапа. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.. 2024;:110-111..

Ninković, Milan, Žutić, Jadranka, Maksimović Zorić, Jelena, Jezdimirović, Nemanja, Milićević, Vesna, "Примена санитарних мера на газдинству у случају појаве слинавке и шапа" in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god. (2024-04-10):110-111.

TY  - JOUR
AU  - Tasic, Aleksandra
AU  - Pezo, Lato
AU  - Lončar, Biljana
AU  - Pešić, Mirjana
AU  - Tešić, Živoslav
AU  - Kalaba, Milica
PY  - 2024
UR  - https://reponivs.nivs.rs/handle/123456789/1006
AB  - This study summarized the physicochemical analysis of 609 honey samples originating
from the Republic of Serbia. Variations among honey samples from different botanical origins,
regions of collections, and harvest years were exposed to descriptive statistics and correlation
analysis that differentiated honey samples. Furthermore, most of the observed physicochemical
parameters (glucose, fructose, sucrose content, 5-hydroxymethylfurfural (5-HMF) levels, acidity, and
electrical conductivity) varied significantly among different types of honey, years, and regions. At the
same time, no noticeable difference was found in diastase activity, moisture content, and insoluble
matter. Based on the obtained results, 22 honey samples could be considered adulterated, due to
the irregular content of sucrose, 5-HMF, acidity, and diastase activity. In addition, 64 honey samples
were suspected to be adulterated. Adulterated and non-compliant samples present a relatively
low percentage (14.1%) of the total number of investigated samples. Consequently, a considerable
number of honey samples met the required standards for honey quality. Overall, these findings
provide insights into compositional and quality differences among various types of honey, aiding in
understanding their characteristics and potential applications.
PB  - Basel : MDPI
T2  - Foods
T1  - Assessing the impact of botanical origins, harvest years, and geographical variability on the physicochemical quality of Serbian honey
IS  - 10
SP  - 1530
VL  - 13
DO  - 10.3390/foods13101530
ER  - 

@article{
author = "Tasic, Aleksandra and Pezo, Lato and Lončar, Biljana and Pešić, Mirjana and Tešić, Živoslav and Kalaba, Milica",
year = "2024",
abstract = "This study summarized the physicochemical analysis of 609 honey samples originating
from the Republic of Serbia. Variations among honey samples from different botanical origins,
regions of collections, and harvest years were exposed to descriptive statistics and correlation
analysis that differentiated honey samples. Furthermore, most of the observed physicochemical
parameters (glucose, fructose, sucrose content, 5-hydroxymethylfurfural (5-HMF) levels, acidity, and
electrical conductivity) varied significantly among different types of honey, years, and regions. At the
same time, no noticeable difference was found in diastase activity, moisture content, and insoluble
matter. Based on the obtained results, 22 honey samples could be considered adulterated, due to
the irregular content of sucrose, 5-HMF, acidity, and diastase activity. In addition, 64 honey samples
were suspected to be adulterated. Adulterated and non-compliant samples present a relatively
low percentage (14.1%) of the total number of investigated samples. Consequently, a considerable
number of honey samples met the required standards for honey quality. Overall, these findings
provide insights into compositional and quality differences among various types of honey, aiding in
understanding their characteristics and potential applications.",
publisher = "Basel : MDPI",
journal = "Foods",
title = "Assessing the impact of botanical origins, harvest years, and geographical variability on the physicochemical quality of Serbian honey",
number = "10",
pages = "1530",
volume = "13",
doi = "10.3390/foods13101530"
}

Tasic, A., Pezo, L., Lončar, B., Pešić, M., Tešić, Ž.,& Kalaba, M.. (2024). Assessing the impact of botanical origins, harvest years, and geographical variability on the physicochemical quality of Serbian honey. in Foods
Basel : MDPI., 13(10), 1530.
https://doi.org/10.3390/foods13101530

Tasic A, Pezo L, Lončar B, Pešić M, Tešić Ž, Kalaba M. Assessing the impact of botanical origins, harvest years, and geographical variability on the physicochemical quality of Serbian honey. in Foods. 2024;13(10):1530.
doi:10.3390/foods13101530 .

Tasic, Aleksandra, Pezo, Lato, Lončar, Biljana, Pešić, Mirjana, Tešić, Živoslav, Kalaba, Milica, "Assessing the impact of botanical origins, harvest years, and geographical variability on the physicochemical quality of Serbian honey" in Foods, 13, no. 10 (2024):1530,
https://doi.org/10.3390/foods13101530 . .

TY  - CONF
AU  - Arsić, Sveta
AU  - Nedić, Sreten
AU  - Prodanović, Radiša
AU  - Bojkovski, Jovan
AU  - Mitrović, Aleksandra
AU  - Ninković, Milan
AU  - Milčić, Staniša
AU  - Savić, Zoran
AU  - Vukomanović, Igor
AU  - Vujanac, Ivan
PY  - 2024
UR  - https://reponivs.nivs.rs/handle/123456789/991
AB  - Dislokacija sirišta je značajan zdravstveni problem u patologi ji digestivnog trakta visokomlečnih krava. Ona predstavlja topografsku gastropatiju 
koja se pojavljuje u više oblika: kao dislokacija sirišta na levo, dislokacija sirišta na de sno i torzija sirišta. Na nivou stada visokomlečnih krava, dislokacija sirišta je široko 
rasprostranjeno oboljenje goveda, čija učestalost pojavljivanja iznosi oko 5%. Od uku pnog broja obolelih životinja, prevalencija levostrane dislokacije iznosi oko 80%, dok se 
ostali oblici promene položaja sirišta pojavljuju ređe. Pored direktnog uticaja na zdrav stveni status i smanjenje proizvodnje mleka kod obolelih životinja, ovo oboljenje ima i 
negativan uticaj na plodnost krava. Tokom poslednjih šezdeset godina, razvijeno je više 
metoda lečenja dislokacije sirišta, od konzervativnih do hiruških tehnika koje daju naj više izgleda za povoljan ishod. Klasične hirurške metode lečenja imaju za cilj repoziciju 
sirišta u anatomski normalan položaj i njegovu fiksaciju za abdominalni zid. Sa razvo jem savremenih tehnika u humanoj medicini, u poslednje dve decenije značajno mesto 
u hirurškom trertmanu dislokacije sirišta zauzimaju i laparoskopske tehnike koje su 
minimalno invazivne. U odnosu na klasične hirurške tehnike, laparoskopska fiksacija 
sirišta ima nekoliko prednosti. Pored manje traume, bolja je abdominalna vizuelizaci ja, jednostavna je za izvođenje, brza, manje stresna po životinju i primenljiva je u svim 
terenskim uslovima
AB  - Displaced abomasum is a significant problem in pathology of the di gestive system of high-yielding cows. Displaced abomasum represents a topographical 
gastropathy that occurs in multiple forms: left displaced abomasum, right displaced 
abomasum and rotated abomasum. In dairy cow herds, displaced abomasum is wide spread pathology with a frequency of occurrence of 5%. The prevalence of left displaced 
abomasum is about 80% of the affected animals, while other forms appear less fre quently. In addition to the direct effect on the health status and the reduction in milk 
production of the affected animals, this disease also has a negative impact on the fer tility of cows. Over the last 60 years, several methods for treating displaced abomasum 
have been developed, from conservative to surgical techniques, the latter having the 
best chance of a favorable outcome. Classic surgical techniques aim to return the dis placed abomasum to its anatomically normal position and fix it to the abdominal wall. 
Parallel to the development of surgical techniques in human medicine, laparoscopic 
treatment, which is minimally invasive, has taken an important place in the surgical 
treatment of displaced abomasum in the last 10 years. Compared to the classic surgical 
techniques, laparoscopic fixation of the displaced abomasum has several advantages. 
In addition to being less traumatic, the abdominal visualization is better, the procedure 
is simpler, quicker and less stressful for the animal, and the method is applicable in the 
field practice.
PB  - Beograd : Udruženje veterinara praktičara Srbije
C3  - Zbornik predavanja : VI Simpozijum sa međunarodnim učešćem “Zdravstvena zaštita i reprodukcija papkara, kopitara, živine i mesojeda”, 18-20. april 2024. godine, Beograd
T1  - Uspešnost različitih tretmana repozicije dislociranog sirišta kod krava
T1  - the success of different treatments for the reposition of displaced abomasum in cows
EP  - 50
SP  - 41
ER  - 

@conference{
author = "Arsić, Sveta and Nedić, Sreten and Prodanović, Radiša and Bojkovski, Jovan and Mitrović, Aleksandra and Ninković, Milan and Milčić, Staniša and Savić, Zoran and Vukomanović, Igor and Vujanac, Ivan",
year = "2024",
abstract = "Dislokacija sirišta je značajan zdravstveni problem u patologi ji digestivnog trakta visokomlečnih krava. Ona predstavlja topografsku gastropatiju 
koja se pojavljuje u više oblika: kao dislokacija sirišta na levo, dislokacija sirišta na de sno i torzija sirišta. Na nivou stada visokomlečnih krava, dislokacija sirišta je široko 
rasprostranjeno oboljenje goveda, čija učestalost pojavljivanja iznosi oko 5%. Od uku pnog broja obolelih životinja, prevalencija levostrane dislokacije iznosi oko 80%, dok se 
ostali oblici promene položaja sirišta pojavljuju ređe. Pored direktnog uticaja na zdrav stveni status i smanjenje proizvodnje mleka kod obolelih životinja, ovo oboljenje ima i 
negativan uticaj na plodnost krava. Tokom poslednjih šezdeset godina, razvijeno je više 
metoda lečenja dislokacije sirišta, od konzervativnih do hiruških tehnika koje daju naj više izgleda za povoljan ishod. Klasične hirurške metode lečenja imaju za cilj repoziciju 
sirišta u anatomski normalan položaj i njegovu fiksaciju za abdominalni zid. Sa razvo jem savremenih tehnika u humanoj medicini, u poslednje dve decenije značajno mesto 
u hirurškom trertmanu dislokacije sirišta zauzimaju i laparoskopske tehnike koje su 
minimalno invazivne. U odnosu na klasične hirurške tehnike, laparoskopska fiksacija 
sirišta ima nekoliko prednosti. Pored manje traume, bolja je abdominalna vizuelizaci ja, jednostavna je za izvođenje, brza, manje stresna po životinju i primenljiva je u svim 
terenskim uslovima, Displaced abomasum is a significant problem in pathology of the di gestive system of high-yielding cows. Displaced abomasum represents a topographical 
gastropathy that occurs in multiple forms: left displaced abomasum, right displaced 
abomasum and rotated abomasum. In dairy cow herds, displaced abomasum is wide spread pathology with a frequency of occurrence of 5%. The prevalence of left displaced 
abomasum is about 80% of the affected animals, while other forms appear less fre quently. In addition to the direct effect on the health status and the reduction in milk 
production of the affected animals, this disease also has a negative impact on the fer tility of cows. Over the last 60 years, several methods for treating displaced abomasum 
have been developed, from conservative to surgical techniques, the latter having the 
best chance of a favorable outcome. Classic surgical techniques aim to return the dis placed abomasum to its anatomically normal position and fix it to the abdominal wall. 
Parallel to the development of surgical techniques in human medicine, laparoscopic 
treatment, which is minimally invasive, has taken an important place in the surgical 
treatment of displaced abomasum in the last 10 years. Compared to the classic surgical 
techniques, laparoscopic fixation of the displaced abomasum has several advantages. 
In addition to being less traumatic, the abdominal visualization is better, the procedure 
is simpler, quicker and less stressful for the animal, and the method is applicable in the 
field practice.",
publisher = "Beograd : Udruženje veterinara praktičara Srbije",
journal = "Zbornik predavanja : VI Simpozijum sa međunarodnim učešćem “Zdravstvena zaštita i reprodukcija papkara, kopitara, živine i mesojeda”, 18-20. april 2024. godine, Beograd",
title = "Uspešnost različitih tretmana repozicije dislociranog sirišta kod krava, the success of different treatments for the reposition of displaced abomasum in cows",
pages = "50-41"
}

Arsić, S., Nedić, S., Prodanović, R., Bojkovski, J., Mitrović, A., Ninković, M., Milčić, S., Savić, Z., Vukomanović, I.,& Vujanac, I.. (2024). Uspešnost različitih tretmana repozicije dislociranog sirišta kod krava. in Zbornik predavanja : VI Simpozijum sa međunarodnim učešćem “Zdravstvena zaštita i reprodukcija papkara, kopitara, živine i mesojeda”, 18-20. april 2024. godine, Beograd
Beograd : Udruženje veterinara praktičara Srbije., 41-50.

Arsić S, Nedić S, Prodanović R, Bojkovski J, Mitrović A, Ninković M, Milčić S, Savić Z, Vukomanović I, Vujanac I. Uspešnost različitih tretmana repozicije dislociranog sirišta kod krava. in Zbornik predavanja : VI Simpozijum sa međunarodnim učešćem “Zdravstvena zaštita i reprodukcija papkara, kopitara, živine i mesojeda”, 18-20. april 2024. godine, Beograd. 2024;:41-50..

Arsić, Sveta, Nedić, Sreten, Prodanović, Radiša, Bojkovski, Jovan, Mitrović, Aleksandra, Ninković, Milan, Milčić, Staniša, Savić, Zoran, Vukomanović, Igor, Vujanac, Ivan, "Uspešnost različitih tretmana repozicije dislociranog sirišta kod krava" in Zbornik predavanja : VI Simpozijum sa međunarodnim učešćem “Zdravstvena zaštita i reprodukcija papkara, kopitara, živine i mesojeda”, 18-20. april 2024. godine, Beograd (2024):41-50.

TY  - CONF
AU  - Pavlović, Ivan
AU  - Tasić, Aleksandra
AU  - Pavlović, Marija
AU  - Zdravković, Nemanja
AU  - Bojkovski, Jovan
AU  - Caro-Petrović, Violeta
PY  - 2024
UR  - https://reponivs.nivs.rs/handle/123456789/933
PB  - New York : Liberty Academic Publishing
C3  - 5. International Mediterranean Scientific Research Congress, Mersin, January 13 - 14, 2024
T1  - Measures to control parasitic infections of goats
EP  - 1396
SP  - 1396
ER  - 

@conference{
author = "Pavlović, Ivan and Tasić, Aleksandra and Pavlović, Marija and Zdravković, Nemanja and Bojkovski, Jovan and Caro-Petrović, Violeta",
year = "2024",
publisher = "New York : Liberty Academic Publishing",
journal = "5. International Mediterranean Scientific Research Congress, Mersin, January 13 - 14, 2024",
title = "Measures to control parasitic infections of goats",
pages = "1396-1396"
}

Pavlović, I., Tasić, A., Pavlović, M., Zdravković, N., Bojkovski, J.,& Caro-Petrović, V.. (2024). Measures to control parasitic infections of goats. in 5. International Mediterranean Scientific Research Congress, Mersin, January 13 - 14, 2024
New York : Liberty Academic Publishing., 1396-1396.

Pavlović I, Tasić A, Pavlović M, Zdravković N, Bojkovski J, Caro-Petrović V. Measures to control parasitic infections of goats. in 5. International Mediterranean Scientific Research Congress, Mersin, January 13 - 14, 2024. 2024;:1396-1396..

Pavlović, Ivan, Tasić, Aleksandra, Pavlović, Marija, Zdravković, Nemanja, Bojkovski, Jovan, Caro-Petrović, Violeta, "Measures to control parasitic infections of goats" in 5. International Mediterranean Scientific Research Congress, Mersin, January 13 - 14, 2024 (2024):1396-1396.

TY  - JOUR
AU  - Šiljegović, Sara
AU  - Mouillaud, Théo
AU  - Jiolle, Davy
AU  - Petrić, Dušan
AU  - Ignjatović-Ćupina, Aleksandra
AU  - Vasić, Ana
AU  - Paupy, Christophe
AU  - Kavran, Mihaela
PY  - 2024
UR  - https://reponivs.nivs.rs/handle/123456789/946
AB  - Dirofilaria immitis and D. repens are the two most widespread and important species
of mosquito-borne nematodes, posing a significant threat to veterinary health and particularly
affecting canines and felines. While D. immitis causes cardiopulmonary dirofilariasis, D. repens causes
subcutaneous infections in dogs and other carnivores. Despite the extensive knowledge on these
parasites, little is known about their natural vectors in Serbia. The parasite Setaria tundra, known to
infect deer, has not yet been detected in Serbia but has been documented in neighboring countries.
Thus, the aim of this study was to (i) further map out Dirofilaria sp. hotspots in the Vojvodina
Province and detect S. tundra for the first time, (ii) detect positive mosquito species that can provide
insights into how the nematodes spread in Serbia, and (iii) analyze the blood-fed female mosquitoes
of species found to be infected, in order to identify the potential source of parasite infection. A total of
2902 female mosquitoes were collected across 73 locations during 2021 and 2022. Molecular biology
methods, based on conventional PCR, were used to analyze non-blood-fed (2521 specimens) and
blood-fed (381 specimens) female mosquitos, in order to detect filarial nematode presence and identify
blood-meal sources, respectively. When the parasite genome was detected, the amplicon (cox1 gene,
650 bp fragment) was sent for Sanger sequencing, further confirming the presence of nematodes and
species assignation. D. immitis was detected in three Culex pipiens mosquitoes collected in Zrenjanin
(August 2021) and Glogonj and Svetozar Miletić (both in July 2021). Additionally, Setaria tundra was
detected in Aedes vexans collected in Iđoš (mid-August 2021) and Aedes caspius, which was collected in Mali Iđoš (end of July 2021). This work identifies two new locations where D. immitis occurs
in Vojvodina, and is the first report of S. tundra in Serbian territory. Blood-meal analysis provided
insights into the preferences of mosquitoes that were positive for Dirofilaria sp. and S. tundra.
PB  - Basel . MDPI
T2  - Animals
T1  - Dirofilaria sp. and Blood Meal Analysis in Mosquitoes Collected in Vojvodina and Maˇcva, and the First Report of Setaria tundra (Issaitshikoff & Rajewskaya, 1928) in Serbia
SP  - 1255
VL  - 14
DO  - 10.3390/ani14091255
ER  - 

@article{
author = "Šiljegović, Sara and Mouillaud, Théo and Jiolle, Davy and Petrić, Dušan and Ignjatović-Ćupina, Aleksandra and Vasić, Ana and Paupy, Christophe and Kavran, Mihaela",
year = "2024",
abstract = "Dirofilaria immitis and D. repens are the two most widespread and important species
of mosquito-borne nematodes, posing a significant threat to veterinary health and particularly
affecting canines and felines. While D. immitis causes cardiopulmonary dirofilariasis, D. repens causes
subcutaneous infections in dogs and other carnivores. Despite the extensive knowledge on these
parasites, little is known about their natural vectors in Serbia. The parasite Setaria tundra, known to
infect deer, has not yet been detected in Serbia but has been documented in neighboring countries.
Thus, the aim of this study was to (i) further map out Dirofilaria sp. hotspots in the Vojvodina
Province and detect S. tundra for the first time, (ii) detect positive mosquito species that can provide
insights into how the nematodes spread in Serbia, and (iii) analyze the blood-fed female mosquitoes
of species found to be infected, in order to identify the potential source of parasite infection. A total of
2902 female mosquitoes were collected across 73 locations during 2021 and 2022. Molecular biology
methods, based on conventional PCR, were used to analyze non-blood-fed (2521 specimens) and
blood-fed (381 specimens) female mosquitos, in order to detect filarial nematode presence and identify
blood-meal sources, respectively. When the parasite genome was detected, the amplicon (cox1 gene,
650 bp fragment) was sent for Sanger sequencing, further confirming the presence of nematodes and
species assignation. D. immitis was detected in three Culex pipiens mosquitoes collected in Zrenjanin
(August 2021) and Glogonj and Svetozar Miletić (both in July 2021). Additionally, Setaria tundra was
detected in Aedes vexans collected in Iđoš (mid-August 2021) and Aedes caspius, which was collected in Mali Iđoš (end of July 2021). This work identifies two new locations where D. immitis occurs
in Vojvodina, and is the first report of S. tundra in Serbian territory. Blood-meal analysis provided
insights into the preferences of mosquitoes that were positive for Dirofilaria sp. and S. tundra.",
publisher = "Basel . MDPI",
journal = "Animals",
title = "Dirofilaria sp. and Blood Meal Analysis in Mosquitoes Collected in Vojvodina and Maˇcva, and the First Report of Setaria tundra (Issaitshikoff & Rajewskaya, 1928) in Serbia",
pages = "1255",
volume = "14",
doi = "10.3390/ani14091255"
}

Šiljegović, S., Mouillaud, T., Jiolle, D., Petrić, D., Ignjatović-Ćupina, A., Vasić, A., Paupy, C.,& Kavran, M.. (2024). Dirofilaria sp. and Blood Meal Analysis in Mosquitoes Collected in Vojvodina and Maˇcva, and the First Report of Setaria tundra (Issaitshikoff & Rajewskaya, 1928) in Serbia. in Animals
Basel . MDPI., 14, 1255.
https://doi.org/10.3390/ani14091255

Šiljegović S, Mouillaud T, Jiolle D, Petrić D, Ignjatović-Ćupina A, Vasić A, Paupy C, Kavran M. Dirofilaria sp. and Blood Meal Analysis in Mosquitoes Collected in Vojvodina and Maˇcva, and the First Report of Setaria tundra (Issaitshikoff & Rajewskaya, 1928) in Serbia. in Animals. 2024;14:1255.
doi:10.3390/ani14091255 .

Šiljegović, Sara, Mouillaud, Théo, Jiolle, Davy, Petrić, Dušan, Ignjatović-Ćupina, Aleksandra, Vasić, Ana, Paupy, Christophe, Kavran, Mihaela, "Dirofilaria sp. and Blood Meal Analysis in Mosquitoes Collected in Vojvodina and Maˇcva, and the First Report of Setaria tundra (Issaitshikoff & Rajewskaya, 1928) in Serbia" in Animals, 14 (2024):1255,
https://doi.org/10.3390/ani14091255 . .

TY  - JOUR
AU  - Zmejoski, Danica Z.
AU  - Zdravković, Nemanja M.
AU  - Budimir Filimonović, Milica D.
AU  - Pavlović, Vladimir B.
AU  - Butulija, Svetlana V.
AU  - Milivojević, Dušan D.
AU  - Marković, Zoran M.
AU  - Todorović Marković, Biljana M.
PY  - 2024
UR  - https://reponivs.nivs.rs/handle/123456789/926
AB  - In this study, nanochitosan dots (ChiDs) were synthesized using gamma rays and encapsulated
in bacterial cellulose (BC) polymer matrix for antibiofilm potential in photodynamic
therapy. The composites were analyzed for structural changes using SEM, AFM, FTIR, XRD, EPR,
and porosity measurements. Additionally, ChiD release was assessed. The results showed that the
chemical composition remained unaltered, but ChiD agglomerates embedded in BC changed shape
(1.5–2.5 μm). Bacterial cellulose fibers became deformed and interconnected, with increased surface
roughness and porosity and decreased crystallinity. No singlet oxygen formation was observed,
and the total amount of released ChiD was up to 16.10%. Antibiofilm activity was higher under
green light, with reductions ranging from 48 to 57% under blue light and 78 to 85% under green
light. Methicillin-resistant Staphylococcus aureus was the most sensitive strain. The new photoactive
composite hydrogels show promising potential for combating biofilm-related infections.
PB  - Basel : MDPI
T2  - Journal of Functional Biomaterials
T1  - Reduction in pathogenic biofilms by the photoactive composite of bacterial cellulose and nanochitosan dots under blue and green light
IS  - 3
SP  - 72
VL  - 15
DO  - 10.3390/ jfb15030072
ER  - 

@article{
author = "Zmejoski, Danica Z. and Zdravković, Nemanja M. and Budimir Filimonović, Milica D. and Pavlović, Vladimir B. and Butulija, Svetlana V. and Milivojević, Dušan D. and Marković, Zoran M. and Todorović Marković, Biljana M.",
year = "2024",
abstract = "In this study, nanochitosan dots (ChiDs) were synthesized using gamma rays and encapsulated
in bacterial cellulose (BC) polymer matrix for antibiofilm potential in photodynamic
therapy. The composites were analyzed for structural changes using SEM, AFM, FTIR, XRD, EPR,
and porosity measurements. Additionally, ChiD release was assessed. The results showed that the
chemical composition remained unaltered, but ChiD agglomerates embedded in BC changed shape
(1.5–2.5 μm). Bacterial cellulose fibers became deformed and interconnected, with increased surface
roughness and porosity and decreased crystallinity. No singlet oxygen formation was observed,
and the total amount of released ChiD was up to 16.10%. Antibiofilm activity was higher under
green light, with reductions ranging from 48 to 57% under blue light and 78 to 85% under green
light. Methicillin-resistant Staphylococcus aureus was the most sensitive strain. The new photoactive
composite hydrogels show promising potential for combating biofilm-related infections.",
publisher = "Basel : MDPI",
journal = "Journal of Functional Biomaterials",
title = "Reduction in pathogenic biofilms by the photoactive composite of bacterial cellulose and nanochitosan dots under blue and green light",
number = "3",
pages = "72",
volume = "15",
doi = "10.3390/ jfb15030072"
}

Zmejoski, D. Z., Zdravković, N. M., Budimir Filimonović, M. D., Pavlović, V. B., Butulija, S. V., Milivojević, D. D., Marković, Z. M.,& Todorović Marković, B. M.. (2024). Reduction in pathogenic biofilms by the photoactive composite of bacterial cellulose and nanochitosan dots under blue and green light. in Journal of Functional Biomaterials
Basel : MDPI., 15(3), 72.
https://doi.org/10.3390/ jfb15030072

Zmejoski DZ, Zdravković NM, Budimir Filimonović MD, Pavlović VB, Butulija SV, Milivojević DD, Marković ZM, Todorović Marković BM. Reduction in pathogenic biofilms by the photoactive composite of bacterial cellulose and nanochitosan dots under blue and green light. in Journal of Functional Biomaterials. 2024;15(3):72.
doi:10.3390/ jfb15030072 .

Zmejoski, Danica Z., Zdravković, Nemanja M., Budimir Filimonović, Milica D., Pavlović, Vladimir B., Butulija, Svetlana V., Milivojević, Dušan D., Marković, Zoran M., Todorović Marković, Biljana M., "Reduction in pathogenic biofilms by the photoactive composite of bacterial cellulose and nanochitosan dots under blue and green light" in Journal of Functional Biomaterials, 15, no. 3 (2024):72,
https://doi.org/10.3390/ jfb15030072 . .

TY  - CONF
AU  - Jezdimirović, Nemanja
AU  - Kureljušić, Branislav
AU  - Savić, Božidar
AU  - Milovanović, Bojan
AU  - Glišić, Dimitrije
AU  - Maletić, Jelena
AU  - Šolaja, Sofija
AU  - Milićević, Vesna
PY  - 2024
UR  - https://reponivs.nivs.rs/handle/123456789/952
AB  - Дивље свиње могу бити резервоари различитих бактерија, вируса и паразита и
могу се пренети на домаће свиње па и на људе индиректним или директним
контактом. Порцине цитомегаловирус (ПЦМВ) препознат је као главни
узрочник атрофичног ринитиса код свиња свих старосних категорија.
Међународни комитет за таксономију вируса (ИТЦВ) је порцине
цитомегаловирус сврстао у suid betaherpesvirus 2 (SuBHV2), и припада
подфамилији Betaherpesvirinae и фамилији Herpesviridae, али није сврстан ни у
један род. Генетска истраживања су показала да је ПЦМВ генетски сроднији
хуманим херпес вирусима (ХХВ) 6А, 6Б и 7. Поред тога, доказано је да ПЦМВ
доприноси репродуктивним проблемима код крмача и резултира раним
неонаталним губицима. Инфекција ПЦМВ-ом је раширена широм света и има
високу преваленцију, посебно у земљама са интензивном производњом свиња.
Клинички пролази непримећено због стеченог имунитета запата, и обично се
јавља код млађих категорија животиња. Нажалост, сама болест и инфекција су
углавном занемарени.
Како бисмо открили улогу дивљих свиња у епидемиологији ПЦМВ-а,
користили смо реал-тиме ПЦР тестирање 50 узорака (лимфни чвор, слезина и
бубрег) пореклом од одстрељених дивљих свиња.
Испитивање је потврдило присуство генома ПЦМВ у 4/50 (8%) узорака. Ови
резултати указују на релативно ниску али не и занемарљиву преваленцију
вируса у популацији дивљих свиња, што може да представља значајан ризик и
за популацију домаћих свиња.
AB  - Wild boars can be reservoirs of various bacteria, viruses, and parasites and can be
transmitted to domestic pigs and even humans through indirect or direct contact.
Porcine cytomegalovirus (PCMV) is recognized as the main cause of atrophic rhinitis
in pigs of all age categories. The International Committee on Taxonomy of Viruses
(ICTV) has classified porcine cytomegalovirus into suid betaherpesvirus 2
(SuBHV2), belonging to the subfamily Betaherpesvirinae and family Herpesviridae,
but it is not classified into any genus. Genetic research has shown that PCMV is
genetically closer to human herpes viruses (HHV) 6A, 6B, and 7. Additionally, it has
been proven that PCMV contributes to reproductive problems in sows and results in
early neonatal losses. PCMV infection is widespread worldwide and has a high
prevalence, especially in countries with intensive pig production. Clinically, it often
goes unnoticed due to acquired maternal immunity and usually occurs in younger
animal categories. Unfortunately, the disease itself and the infection are largely
neglected.
To discover the role of wild boars in PCMV epidemiology, we used real-time PCR
testing of 50 samples (lymph nodes, spleen, and kidney) originating from shot wild
boars.
Testing confirmed the presence of PCMV genome in 4 out of 50 (8%) samples. These
results indicate a relatively low but not negligible prevalence of the virus in the wild
boar population, which may pose a significant risk to the domestic pig population.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
T1  - Prva molekularna detekcija porcine citomegalovirusa kod divljih svinja u Srbiji
T1  - First molecular detection of porcine cytomegalovirus in wild boars in Serbia
EP  - 79
SP  - 78
ER  - 

@conference{
author = "Jezdimirović, Nemanja and Kureljušić, Branislav and Savić, Božidar and Milovanović, Bojan and Glišić, Dimitrije and Maletić, Jelena and Šolaja, Sofija and Milićević, Vesna",
year = "2024",
abstract = "Дивље свиње могу бити резервоари различитих бактерија, вируса и паразита и
могу се пренети на домаће свиње па и на људе индиректним или директним
контактом. Порцине цитомегаловирус (ПЦМВ) препознат је као главни
узрочник атрофичног ринитиса код свиња свих старосних категорија.
Међународни комитет за таксономију вируса (ИТЦВ) је порцине
цитомегаловирус сврстао у suid betaherpesvirus 2 (SuBHV2), и припада
подфамилији Betaherpesvirinae и фамилији Herpesviridae, али није сврстан ни у
један род. Генетска истраживања су показала да је ПЦМВ генетски сроднији
хуманим херпес вирусима (ХХВ) 6А, 6Б и 7. Поред тога, доказано је да ПЦМВ
доприноси репродуктивним проблемима код крмача и резултира раним
неонаталним губицима. Инфекција ПЦМВ-ом је раширена широм света и има
високу преваленцију, посебно у земљама са интензивном производњом свиња.
Клинички пролази непримећено због стеченог имунитета запата, и обично се
јавља код млађих категорија животиња. Нажалост, сама болест и инфекција су
углавном занемарени.
Како бисмо открили улогу дивљих свиња у епидемиологији ПЦМВ-а,
користили смо реал-тиме ПЦР тестирање 50 узорака (лимфни чвор, слезина и
бубрег) пореклом од одстрељених дивљих свиња.
Испитивање је потврдило присуство генома ПЦМВ у 4/50 (8%) узорака. Ови
резултати указују на релативно ниску али не и занемарљиву преваленцију
вируса у популацији дивљих свиња, што може да представља значајан ризик и
за популацију домаћих свиња., Wild boars can be reservoirs of various bacteria, viruses, and parasites and can be
transmitted to domestic pigs and even humans through indirect or direct contact.
Porcine cytomegalovirus (PCMV) is recognized as the main cause of atrophic rhinitis
in pigs of all age categories. The International Committee on Taxonomy of Viruses
(ICTV) has classified porcine cytomegalovirus into suid betaherpesvirus 2
(SuBHV2), belonging to the subfamily Betaherpesvirinae and family Herpesviridae,
but it is not classified into any genus. Genetic research has shown that PCMV is
genetically closer to human herpes viruses (HHV) 6A, 6B, and 7. Additionally, it has
been proven that PCMV contributes to reproductive problems in sows and results in
early neonatal losses. PCMV infection is widespread worldwide and has a high
prevalence, especially in countries with intensive pig production. Clinically, it often
goes unnoticed due to acquired maternal immunity and usually occurs in younger
animal categories. Unfortunately, the disease itself and the infection are largely
neglected.
To discover the role of wild boars in PCMV epidemiology, we used real-time PCR
testing of 50 samples (lymph nodes, spleen, and kidney) originating from shot wild
boars.
Testing confirmed the presence of PCMV genome in 4 out of 50 (8%) samples. These
results indicate a relatively low but not negligible prevalence of the virus in the wild
boar population, which may pose a significant risk to the domestic pig population.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.",
title = "Prva molekularna detekcija porcine citomegalovirusa kod divljih svinja u Srbiji, First molecular detection of porcine cytomegalovirus in wild boars in Serbia",
pages = "79-78"
}

Jezdimirović, N., Kureljušić, B., Savić, B., Milovanović, B., Glišić, D., Maletić, J., Šolaja, S.,& Milićević, V.. (2024). Prva molekularna detekcija porcine citomegalovirusa kod divljih svinja u Srbiji. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 78-79.

Jezdimirović N, Kureljušić B, Savić B, Milovanović B, Glišić D, Maletić J, Šolaja S, Milićević V. Prva molekularna detekcija porcine citomegalovirusa kod divljih svinja u Srbiji. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.. 2024;:78-79..

Jezdimirović, Nemanja, Kureljušić, Branislav, Savić, Božidar, Milovanović, Bojan, Glišić, Dimitrije, Maletić, Jelena, Šolaja, Sofija, Milićević, Vesna, "Prva molekularna detekcija porcine citomegalovirusa kod divljih svinja u Srbiji" in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god. (2024):78-79.

TY  - CONF
AU  - Savić, Božidar
AU  - Milićević, Vesna
AU  - Kureljušić, Branislav
AU  - Jezdimirović, Nemanja
AU  - Milovanović, Bojan
AU  - Maksimović Zorić, Jelena
AU  - Stevnčević, Ognjen
PY  - 2024
UR  - https://reponivs.nivs.rs/handle/123456789/953
AB  - Репродуктивни и респираторни синдром свиња (енгл. Porcine Reproductive and Respiratory
Syndrome – PRRS), је контагиозна вирусна афекција свиња која тренутно представља
економски најзначајније инфективно обољење свиња, ендемично у многим земљама у
свету укључујучи и нашу земљу. PRRS је узрокован са вирусом репродуктивног и
респираторног синдрома свиња (енгл. Porcine Reproductive and Respiratory Syndrome Virus
– PRRSV), а с краја 90-их година прошлог века, када је обољење по први пут
дијагностиковано у нашој земљи, постоји релативно ограничен број епидемиолошких
студија овог обољења и молекуларних истраживања овог вируса који циркулишу у
популацији свиња. Описана су два генотипа PRRSV: тип-1 или Европски тип (Lelystad -
прототип) и тип-2 или Амерички тип (VR-2332 – прототип). Од недавно, PRRSV тип-1 и
тип-2 су класификовани као две засебне врсте унутар рода Betaarterivirus и то као
Betaarterivirus suid 1 (PRRSV1) и Betaarterivirus suid 2 (PRRSV2). У оквиру генотипа 1
(PRRSV1) се разликују 4 генетске линије (подтипа) (1, 2, 3 и 4) у које су сврстани изолати
вируса пореклом из различитих географских региона Европе. Унутар подтипова 1 и 3, се
диференцирају кластери 1А-1Г и 3А-3Г док подтип 2 чини јединствена група изолата, без
дефинисаних кластера. У нашој земљи, PRRS је био искључиво узрокован генотипом 1
(PRRSV1), међутим, 2022 године, детектован је и генотип 2 вируса (PRRSV2), на фарми
која је вршила увоз свиња из Европске уније. Анализом генома овог вируса је установљено
да је најсличнији тзв. вакциналним-сојевима (vaccine-like strain), односно да је вирус
највероватније пореклом из вакцине (МLV PRRSV2 вакцина) која је употребљена за
имунизацију свиња против PRRSV2 у земљи извозници. Циљ овог истраживањ је био да
се установи генетски диверзитет изолата PRRSV1 пореклом са фарми свиња из наше земље
у периоду од 2012 до 2022 године. У ту сврху, извршено је секвенцирање 36 ОРФ5 гена
изолата PRRSV1 коришћењем Сангер методе, које су потом поређене и анализиране
заједно са 106 секвенци ОРФ5 гена PRRSV1 из генетских линија 1, 2 и 3, које репрезентују
комплетан спектар генетског диверзитета ових подтипова (секвенце кластера 1А-1Г, 3А-
3Г и линије 2), применом различитих модула са предефинисаним параметарима у оквиру
софтверских пакета CLC – MainWorkbench и Mega X. Прикупљене секвнеце ОРФ5 гена су
депоноване у „NCBI“ бази биоинформатичких података. Филогенетском анализом је
установљено да су сви изолати PRRSV1 детектовани у периооду од 2012 до 2022 године у
нашој земљи груписани у два подтипа, односно подтип 1 и 3. У оквиру линије 1, се налази
7 изолата и то: 2 изолата у кластеру 1А, 2 у кластеру 1Б, 2 у кластеру 1Д и један изолат у
кластеру 1Ф. У оквиру линије 2, 29 секвенци је груписано у кластер 3Ц. Добијени
резултати представљају важне информације за будуће епидемиолошке анализе PRRSV1
инфекције укључујући утврђивање повезаности односно сродности између изолата вируса,
поређење нових секвенци са секвенцама пореклом од вакциналних сојева вируса,
разликовање вакциналних од „дивљих“ сојева вируса и утврђивање „уноса“ нових вируса
у популацију. Оваква епидемиолошка истраживања треба искористити за установљавање
критичних тачака у биосигурносним мерама на конкретним фармама, те имплементирати
одговарајуће промене како би се PRRSV инфекција успешно превенирала и контролисала.
AB  - Porcine Reproductive and Respiratory Syndrome (PRRS) is a contagious viral disease
of swine that currently represents the most economically significant infectious disease
of pigs, endemic in many countries worldwide, including Serbia. PRRS is caused by the
Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). Since the late 1990s,
when the disease was first diagnosed in the Serbia, there have been only few
epidemiological and molecular studies on circulating PPRS-viruses. Two genotypes of
PRRSV have been described: type 1 or European type (prototype Lelystad) and type 2
or American type (prototype VR-2332). Recently, PRRSV type 1 and type 2 have been
classified as two separate species within the genus Betaarterivirus, namely
Betaarterivirus suid 1 (PRRSV1) and Betaarterivirus suid 2 (PRRSV2). Within
genotype 1 (PRRSV1), four genetic lineages (subtypes) (1, 2, 3, and 4) have been
distinguished, in which isolates from different geographic regions of Europe are
classified. Within subtypes 1 and 3, clusters 1A-1G and 3A-3G are further differentiated,
while subtype 2 consists of a unique group of isolates, without defined clusters. In Serbia,
PRRS was exclusively caused by genotype 1 (PRRSV1); however, in 2022, a genotype
2 (PRRSV2) was detected on a farm that imported pigs from the European Union.
Genome analysis of this virus revealed its similarity to vaccine-like strains, indicating
that the virus most likely originated from a vaccine (MLV PRRSV2 vaccine) used for
immunization against PRRSV2 in the exporting country.
The aim of this study was to establish the genetic diversity of PRRSV1 isolates
originating from pig farms in Serbia from 2012 to 2022. For this purpose, sequencing of
36 ORF5 gene of PRRSV1 was performed using the Sanger method, which were then
compared and analyzed together with 106 ORF5 gene sequences of PRRSV1 from
genetic lineages 1, 2, and 3, representing the full range of genetic diversity of this
subtype (sequences from 1A-1G, 3A-3G clusters, and lineage 2), by using different
modules with predefined parameters within the software packages CLC –
MainWorkbench and Mega X. The obtained ORF5 gene sequences were deposited in the
NCBI database. Phylogenetic analysis revealed that all PRRSV1 isolates detected in the
Serbia from 2012 to 2022 were grouped into two subtypes, subtype 1 and 3. Within
lineage 1, there were 7 isolates: 2 in cluster 1A, 2 in cluster 1B, 2 in cluster 1D, and one
isolate in cluster 1F. Within lineage 2, 29 sequences were grouped in cluster 3C.
The obtained results provide important information for future epidemiological studies of
PRRSV1 infection, including the relatedness between isolates, comparing new obtianed
sequences with sequences originating from vaccine strains, differentiating vaccine
strains from "wild" strains, and determining the introduction of new strains into the
population. Such epidemiological studies can be used for find critical points in the
biosecurity on the given farms and implement changes in the others to prevent and
control PRRSV infection.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
T1  - Genetski diverzitet ORF5 gena virusa reproduktivnog i respiratornog sindroma svinja tip 1 od 2012 do 2022 godine
T1  - Genetic diversity of the ORF5 gene of type 1 porcine reproductive and respiratory syndrome virus in Serbia from 2012 to 2022
EP  - 103
SP  - 102
ER  - 

@conference{
author = "Savić, Božidar and Milićević, Vesna and Kureljušić, Branislav and Jezdimirović, Nemanja and Milovanović, Bojan and Maksimović Zorić, Jelena and Stevnčević, Ognjen",
year = "2024",
abstract = "Репродуктивни и респираторни синдром свиња (енгл. Porcine Reproductive and Respiratory
Syndrome – PRRS), је контагиозна вирусна афекција свиња која тренутно представља
економски најзначајније инфективно обољење свиња, ендемично у многим земљама у
свету укључујучи и нашу земљу. PRRS је узрокован са вирусом репродуктивног и
респираторног синдрома свиња (енгл. Porcine Reproductive and Respiratory Syndrome Virus
– PRRSV), а с краја 90-их година прошлог века, када је обољење по први пут
дијагностиковано у нашој земљи, постоји релативно ограничен број епидемиолошких
студија овог обољења и молекуларних истраживања овог вируса који циркулишу у
популацији свиња. Описана су два генотипа PRRSV: тип-1 или Европски тип (Lelystad -
прототип) и тип-2 или Амерички тип (VR-2332 – прототип). Од недавно, PRRSV тип-1 и
тип-2 су класификовани као две засебне врсте унутар рода Betaarterivirus и то као
Betaarterivirus suid 1 (PRRSV1) и Betaarterivirus suid 2 (PRRSV2). У оквиру генотипа 1
(PRRSV1) се разликују 4 генетске линије (подтипа) (1, 2, 3 и 4) у које су сврстани изолати
вируса пореклом из различитих географских региона Европе. Унутар подтипова 1 и 3, се
диференцирају кластери 1А-1Г и 3А-3Г док подтип 2 чини јединствена група изолата, без
дефинисаних кластера. У нашој земљи, PRRS је био искључиво узрокован генотипом 1
(PRRSV1), међутим, 2022 године, детектован је и генотип 2 вируса (PRRSV2), на фарми
која је вршила увоз свиња из Европске уније. Анализом генома овог вируса је установљено
да је најсличнији тзв. вакциналним-сојевима (vaccine-like strain), односно да је вирус
највероватније пореклом из вакцине (МLV PRRSV2 вакцина) која је употребљена за
имунизацију свиња против PRRSV2 у земљи извозници. Циљ овог истраживањ је био да
се установи генетски диверзитет изолата PRRSV1 пореклом са фарми свиња из наше земље
у периоду од 2012 до 2022 године. У ту сврху, извршено је секвенцирање 36 ОРФ5 гена
изолата PRRSV1 коришћењем Сангер методе, које су потом поређене и анализиране
заједно са 106 секвенци ОРФ5 гена PRRSV1 из генетских линија 1, 2 и 3, које репрезентују
комплетан спектар генетског диверзитета ових подтипова (секвенце кластера 1А-1Г, 3А-
3Г и линије 2), применом различитих модула са предефинисаним параметарима у оквиру
софтверских пакета CLC – MainWorkbench и Mega X. Прикупљене секвнеце ОРФ5 гена су
депоноване у „NCBI“ бази биоинформатичких података. Филогенетском анализом је
установљено да су сви изолати PRRSV1 детектовани у периооду од 2012 до 2022 године у
нашој земљи груписани у два подтипа, односно подтип 1 и 3. У оквиру линије 1, се налази
7 изолата и то: 2 изолата у кластеру 1А, 2 у кластеру 1Б, 2 у кластеру 1Д и један изолат у
кластеру 1Ф. У оквиру линије 2, 29 секвенци је груписано у кластер 3Ц. Добијени
резултати представљају важне информације за будуће епидемиолошке анализе PRRSV1
инфекције укључујући утврђивање повезаности односно сродности између изолата вируса,
поређење нових секвенци са секвенцама пореклом од вакциналних сојева вируса,
разликовање вакциналних од „дивљих“ сојева вируса и утврђивање „уноса“ нових вируса
у популацију. Оваква епидемиолошка истраживања треба искористити за установљавање
критичних тачака у биосигурносним мерама на конкретним фармама, те имплементирати
одговарајуће промене како би се PRRSV инфекција успешно превенирала и контролисала., Porcine Reproductive and Respiratory Syndrome (PRRS) is a contagious viral disease
of swine that currently represents the most economically significant infectious disease
of pigs, endemic in many countries worldwide, including Serbia. PRRS is caused by the
Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). Since the late 1990s,
when the disease was first diagnosed in the Serbia, there have been only few
epidemiological and molecular studies on circulating PPRS-viruses. Two genotypes of
PRRSV have been described: type 1 or European type (prototype Lelystad) and type 2
or American type (prototype VR-2332). Recently, PRRSV type 1 and type 2 have been
classified as two separate species within the genus Betaarterivirus, namely
Betaarterivirus suid 1 (PRRSV1) and Betaarterivirus suid 2 (PRRSV2). Within
genotype 1 (PRRSV1), four genetic lineages (subtypes) (1, 2, 3, and 4) have been
distinguished, in which isolates from different geographic regions of Europe are
classified. Within subtypes 1 and 3, clusters 1A-1G and 3A-3G are further differentiated,
while subtype 2 consists of a unique group of isolates, without defined clusters. In Serbia,
PRRS was exclusively caused by genotype 1 (PRRSV1); however, in 2022, a genotype
2 (PRRSV2) was detected on a farm that imported pigs from the European Union.
Genome analysis of this virus revealed its similarity to vaccine-like strains, indicating
that the virus most likely originated from a vaccine (MLV PRRSV2 vaccine) used for
immunization against PRRSV2 in the exporting country.
The aim of this study was to establish the genetic diversity of PRRSV1 isolates
originating from pig farms in Serbia from 2012 to 2022. For this purpose, sequencing of
36 ORF5 gene of PRRSV1 was performed using the Sanger method, which were then
compared and analyzed together with 106 ORF5 gene sequences of PRRSV1 from
genetic lineages 1, 2, and 3, representing the full range of genetic diversity of this
subtype (sequences from 1A-1G, 3A-3G clusters, and lineage 2), by using different
modules with predefined parameters within the software packages CLC –
MainWorkbench and Mega X. The obtained ORF5 gene sequences were deposited in the
NCBI database. Phylogenetic analysis revealed that all PRRSV1 isolates detected in the
Serbia from 2012 to 2022 were grouped into two subtypes, subtype 1 and 3. Within
lineage 1, there were 7 isolates: 2 in cluster 1A, 2 in cluster 1B, 2 in cluster 1D, and one
isolate in cluster 1F. Within lineage 2, 29 sequences were grouped in cluster 3C.
The obtained results provide important information for future epidemiological studies of
PRRSV1 infection, including the relatedness between isolates, comparing new obtianed
sequences with sequences originating from vaccine strains, differentiating vaccine
strains from "wild" strains, and determining the introduction of new strains into the
population. Such epidemiological studies can be used for find critical points in the
biosecurity on the given farms and implement changes in the others to prevent and
control PRRSV infection.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.",
title = "Genetski diverzitet ORF5 gena virusa reproduktivnog i respiratornog sindroma svinja tip 1 od 2012 do 2022 godine, Genetic diversity of the ORF5 gene of type 1 porcine reproductive and respiratory syndrome virus in Serbia from 2012 to 2022",
pages = "103-102"
}

Savić, B., Milićević, V., Kureljušić, B., Jezdimirović, N., Milovanović, B., Maksimović Zorić, J.,& Stevnčević, O.. (2024). Genetski diverzitet ORF5 gena virusa reproduktivnog i respiratornog sindroma svinja tip 1 od 2012 do 2022 godine. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 102-103.

Savić B, Milićević V, Kureljušić B, Jezdimirović N, Milovanović B, Maksimović Zorić J, Stevnčević O. Genetski diverzitet ORF5 gena virusa reproduktivnog i respiratornog sindroma svinja tip 1 od 2012 do 2022 godine. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.. 2024;:102-103..

Savić, Božidar, Milićević, Vesna, Kureljušić, Branislav, Jezdimirović, Nemanja, Milovanović, Bojan, Maksimović Zorić, Jelena, Stevnčević, Ognjen, "Genetski diverzitet ORF5 gena virusa reproduktivnog i respiratornog sindroma svinja tip 1 od 2012 do 2022 godine" in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god. (2024):102-103.

TY  - CONF
AU  - Maksimović Zorić, Jelena
AU  - Veljović, Ljubiša
AU  - Glišić, Dimitrije
AU  - Kureljušić, Branislav
AU  - Jezdimirović, Nemanja
AU  - Stanojević, Slobodan
AU  - Maletić, Jelena
AU  - Ninković, Milan
AU  - Aksić, Đorđe
AU  - Savić, Božidar
PY  - 2024
UR  - https://reponivs.nivs.rs/handle/123456789/951
AB  - Афричка куга свиња (АКС) је први пут потврђена на територији Републике
Србије у јулу 2019. године, код домаћих свиња на епизоотиолошком подручју
Града Београда. Епизоотиолошким увиђајем је установљено да је неодговорно
понашање људи довело до уноса вируса из околних земаља и ширења заразе. У
првом таласу регистрована су жаришта у три насељена места, а применом
законом прописаних мера, болест је стављена под контролу и одјављена
децембра 2019. године. Упркос поновном уносу вируса из околних земаља и
ширењу заразе у популацијама дивљих и домаћих свиња у североисточним,
централним, источним и јужним деловима земље, ситуација на територији
епизоотиолошког подручја Града Београда у периоду од 2020. до 2022. године
је била стабилна. Поновно избијање заразе десило се 9. јуна 2023. године када
је болест потврђена код угинуле дивље свиње пронађене у ловишту
"Панчевачки рит", на територији општине Палилула. Већ 12. јуна потврђен је и
први случај код екстензивно узгајаних домаћих свиња у насљеном месту
Крњача. Зараза је убрзо потврђена и код дивљих свиња суседног ловишта "Рит"
и домаћих свиња других насељених места општине Палилула (Бесни Фок,
Дунавац, Јабучки Рит, Ковилово, Падинска Скела, Борча, Овча, Сланци, Велико
Село, Вишњица). У периоду од 9. јуна до 5. августа болест је потврђена на 157
газдинстава, односно код 223 домаће и код 110 дивљих свиња, те је посумњано
да су активности људи уз неадекватне биосигурносне мере на газдинствима
допринеле настанку епизоотије. Током јула и августа 2023. године зараза је
потврђена и код дивљих и домаћих свиња на територијама општина Земун,
Гроцка, Чукарица и Обреновац, где је регистровано 34 позитивна газдинстава и
једно ловиште. Од 26. септембра до 28. децембра није било нових случајева
заразе на епизоотиолошком подручју Града Београда, те је зараза одјављена 26.
децембра, односно 3 месеца после последњег потврђеног случаја АКС.
AB  - African swine fever (ASF) was first confirmed in the Republic of Serbia in July 2019,
in domestic pigs settled within the Belgrade epizootiology area. Through an
epizootiological investigation, it was concluded that the outbreak was a result of
irresponsible human behaviour which led to the introduction of the virus from
surrounding countries and its further spread. In the first wave, three outbreaks were
detected in three villages and by implementing regulatory measures for disease
control, the infection was successfully resolved in December 2019. Although the virus
re-entered from neighboring countries and spread across the northeast, central,
eastern, and southern regions, there were no new disease outbreaks in the Belgrade
epizootiological unit during the 2020-2022 period. Despite efforts to control the
infection, the disease reappeared in June 2023, spreading rapidly among wild boars
and domestic pigs in various settlements and hunting grounds of Palilula municipality.
During the two-month period, the disease was confirmed on 157 holdings,
respectively in 223 domestic pigs and 110 wild boars, implying the significance of
human behaviour and poor biosecurity measures on the epizootic spread of the
disease. During July and August 2023, the infection was confirmed in wild boars and
domestic pigs in the municipalities Zemun, Grocka, Čukarica, and Obrenovac, where
34 holdings and one hunting ground were declared positive. From September 26th to
December 28th, there were no new cases of infection in the Belgrade epizootiology
area, and the infection was resolved on December 26th, i.e. 3 months after the last
confirmed case of ASF.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
T1  - Afrička kuga svinja - epizootiološka situacija na teritoriji okruga grada Beograda u periodu od 2019. do 2023. godine
T1  - African swine fever - epizootiological situation in the Belgrade city area in the period from 2019 to 2023
EP  - 91
SP  - 90
ER  - 

@conference{
author = "Maksimović Zorić, Jelena and Veljović, Ljubiša and Glišić, Dimitrije and Kureljušić, Branislav and Jezdimirović, Nemanja and Stanojević, Slobodan and Maletić, Jelena and Ninković, Milan and Aksić, Đorđe and Savić, Božidar",
year = "2024",
abstract = "Афричка куга свиња (АКС) је први пут потврђена на територији Републике
Србије у јулу 2019. године, код домаћих свиња на епизоотиолошком подручју
Града Београда. Епизоотиолошким увиђајем је установљено да је неодговорно
понашање људи довело до уноса вируса из околних земаља и ширења заразе. У
првом таласу регистрована су жаришта у три насељена места, а применом
законом прописаних мера, болест је стављена под контролу и одјављена
децембра 2019. године. Упркос поновном уносу вируса из околних земаља и
ширењу заразе у популацијама дивљих и домаћих свиња у североисточним,
централним, источним и јужним деловима земље, ситуација на територији
епизоотиолошког подручја Града Београда у периоду од 2020. до 2022. године
је била стабилна. Поновно избијање заразе десило се 9. јуна 2023. године када
је болест потврђена код угинуле дивље свиње пронађене у ловишту
"Панчевачки рит", на територији општине Палилула. Већ 12. јуна потврђен је и
први случај код екстензивно узгајаних домаћих свиња у насљеном месту
Крњача. Зараза је убрзо потврђена и код дивљих свиња суседног ловишта "Рит"
и домаћих свиња других насељених места општине Палилула (Бесни Фок,
Дунавац, Јабучки Рит, Ковилово, Падинска Скела, Борча, Овча, Сланци, Велико
Село, Вишњица). У периоду од 9. јуна до 5. августа болест је потврђена на 157
газдинстава, односно код 223 домаће и код 110 дивљих свиња, те је посумњано
да су активности људи уз неадекватне биосигурносне мере на газдинствима
допринеле настанку епизоотије. Током јула и августа 2023. године зараза је
потврђена и код дивљих и домаћих свиња на територијама општина Земун,
Гроцка, Чукарица и Обреновац, где је регистровано 34 позитивна газдинстава и
једно ловиште. Од 26. септембра до 28. децембра није било нових случајева
заразе на епизоотиолошком подручју Града Београда, те је зараза одјављена 26.
децембра, односно 3 месеца после последњег потврђеног случаја АКС., African swine fever (ASF) was first confirmed in the Republic of Serbia in July 2019,
in domestic pigs settled within the Belgrade epizootiology area. Through an
epizootiological investigation, it was concluded that the outbreak was a result of
irresponsible human behaviour which led to the introduction of the virus from
surrounding countries and its further spread. In the first wave, three outbreaks were
detected in three villages and by implementing regulatory measures for disease
control, the infection was successfully resolved in December 2019. Although the virus
re-entered from neighboring countries and spread across the northeast, central,
eastern, and southern regions, there were no new disease outbreaks in the Belgrade
epizootiological unit during the 2020-2022 period. Despite efforts to control the
infection, the disease reappeared in June 2023, spreading rapidly among wild boars
and domestic pigs in various settlements and hunting grounds of Palilula municipality.
During the two-month period, the disease was confirmed on 157 holdings,
respectively in 223 domestic pigs and 110 wild boars, implying the significance of
human behaviour and poor biosecurity measures on the epizootic spread of the
disease. During July and August 2023, the infection was confirmed in wild boars and
domestic pigs in the municipalities Zemun, Grocka, Čukarica, and Obrenovac, where
34 holdings and one hunting ground were declared positive. From September 26th to
December 28th, there were no new cases of infection in the Belgrade epizootiology
area, and the infection was resolved on December 26th, i.e. 3 months after the last
confirmed case of ASF.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.",
title = "Afrička kuga svinja - epizootiološka situacija na teritoriji okruga grada Beograda u periodu od 2019. do 2023. godine, African swine fever - epizootiological situation in the Belgrade city area in the period from 2019 to 2023",
pages = "91-90"
}

Maksimović Zorić, J., Veljović, L., Glišić, D., Kureljušić, B., Jezdimirović, N., Stanojević, S., Maletić, J., Ninković, M., Aksić, Đ.,& Savić, B.. (2024). Afrička kuga svinja - epizootiološka situacija na teritoriji okruga grada Beograda u periodu od 2019. do 2023. godine. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 90-91.

Maksimović Zorić J, Veljović L, Glišić D, Kureljušić B, Jezdimirović N, Stanojević S, Maletić J, Ninković M, Aksić Đ, Savić B. Afrička kuga svinja - epizootiološka situacija na teritoriji okruga grada Beograda u periodu od 2019. do 2023. godine. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.. 2024;:90-91..

Maksimović Zorić, Jelena, Veljović, Ljubiša, Glišić, Dimitrije, Kureljušić, Branislav, Jezdimirović, Nemanja, Stanojević, Slobodan, Maletić, Jelena, Ninković, Milan, Aksić, Đorđe, Savić, Božidar, "Afrička kuga svinja - epizootiološka situacija na teritoriji okruga grada Beograda u periodu od 2019. do 2023. godine" in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god. (2024):90-91.

TY  - CONF
AU  - Kureljušić, Branislav
AU  - Vićić, Ivan
AU  - Bradić, Milijana
AU  - Maletić, Jelena
AU  - Jezdimirović, Nemanja
AU  - Savić, Božidar
AU  - Milovanović, Bojan
AU  - Karabasil, Neđeljko
PY  - 2024
UR  - https://reponivs.nivs.rs/handle/123456789/955
AB  - Интензивирањем бројлерске производње дошло је до значајног повећања
преваленције различитих патолошких стања чија основна етиологија почива у
самом технолошком процесу производње. Појава контактног дерматитиса на
плантарној кожи екстремитета („foot pad dermatitis“), затим на скочном зглобу
(„hock burns“) и на грудима („breast burns“) данас представља врло раширену
појаву у интензивној производњи, наносећи значајне економске губитке услед
смањеног прираста и одбацивања трупова на линији клања. Поред интензивирања
бројлерске производње значајан утицај на њихову појаву имају исхрана, услови и
начин држања (врста простирке) и генетска предиспозиција. Најзначајнији
фактор ризика за настанак лезија је влажна простирка посебно у комбинацији са
штетним дејством амонијака. Појава лезија има веома велики значај као
индикатор добробити имајући у виду да постоје претпоставке да су установљене
лезије врло болне и да често онемогућавају нормално кретање пилића.
Макроскопским прегледом лезија на плантарној површини уочавају се
инфламаторне лезије праћене некрозом на јастучићима табана и/или на прстима.
Лезије се ређе могу установити на тибиометатарзалним зглобовима. Код благог
облика постоји дисколорација на кожи, хиперкератоза, а у тежим случајевима
јавња се едем, ерозије и улцерације.
Хистопатолошки, благе лезије се карактеришу умереном хиперплазијом и/или
хиперкератозом епидермиса, конгестијом и едемом, уз присуство благог
инфламаторног инфилтрата у форми периваскулитиса и субепителијалне
инфилтрације мононуклеарним ћелијама и мањим бројем гранулоцита. Умерене
суперфицијелне лезије се карактеришу врло израженом хиперплазијом и
хиперкератозом епидермиса, егзоцитозом, појавом пустула и красти, конгестијом
и инфилтрацијом дермиса мононуклеарним и гранулоцитиним инфилтратом.
Тешке, дубоке лезије се карактеришу некрозом епидермиса који је замењен
некротичним гнојним садржајем, појавом гранулационог ткива, израженом
инфламаторном инфилтрацијом (периваскулитис и субепителна инфилтрација)
доминантно хетерофилним гранулоцитима и мононуклеарним ћелијама.
Имајући у виду да је појава контактног дерматитиса све учесталија појава како на
фармама бројлерских пилића тако и на кланици приликом прегледа трупова на
линија клања неопходно је имплементирати програм контроле овог патолошког
стања и на тај начин побољшати добробит и обезбедити производњу квалитетног
и безбедног пилећег меса.
AB  - The intensification of broiler production has led to a significant increase in the
prevalence of various pathological conditions, whose basic etiology lies in the
production process itself. The occurrence of contact dermatitis on the plantar skin of
extremities ("foot pad dermatitis"), then on the hock joint ("hock burns"), and on the
breasts ("breast burns") is today a very widespread phenomenon in intensive production,
causing significant economic losses due to reduced growth and carcass rejection on the
slaughter line. In addition to the intensification of broiler production, significant factors
influencing their occurrence are nutrition, housing conditions (type of bedding), and
genetic predisposition. The most significant risk factor for lesion formation is wet
bedding, especially in combination with the harmful effects of ammonia. The
occurrence of lesions is very important as an indicator of welfare, considering that there
are assumptions that established lesions are very painful and often prevent normal
chicken movement. Macroscopic examination of lesions on the plantar surface reveals
inflammatory lesions accompanied by necrosis on the foot pads and/or toes. Lesions are
less commonly found on the tibiotarsal joints. In mild forms, discoloration of the skin,
hyperkeratosis, and in severe cases, edema, erosions, and ulcerations occur.
Histopathologically, mild lesions are characterized by moderate epidermal hyperplasia
and/or hyperkeratosis, congestion and edema, with the presence of mild inflammatory
infiltrate in the form of perivasculitis and subepithelial infiltration of mononuclear cells
and a small number of granulocytes. Moderate superficial lesions are characterized by
very pronounced epidermal hyperplasia and hyperkeratosis, exocytosis, appearance of
pustules and crusts, congestion, and infiltration of the dermis with mononuclear and
granulocytic infiltrate. Severe, deep lesions are characterized by epidermal necrosis
replaced by necrotic purulent content, appearance of granulation tissue, pronounced
inflammatory infiltration (perivasculitis and subepithelial infiltration) predominantly
with heterophilic granulocytes and mononuclear cells. Considering that the occurrence
of contact dermatitis is becoming more frequent both on broiler farms and in
slaughterhouses during carcass examination on the slaughter line, it is necessary to
implement a control program for this pathological condition
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
T1  - Kontaktni pododermatitis kod brojlerskih pilića – patološki aspekt -
T1  - Contact pododermatitis in broiler chickens – pathological aspect –
EP  - 147
SP  - 146
ER  - 

@conference{
author = "Kureljušić, Branislav and Vićić, Ivan and Bradić, Milijana and Maletić, Jelena and Jezdimirović, Nemanja and Savić, Božidar and Milovanović, Bojan and Karabasil, Neđeljko",
year = "2024",
abstract = "Интензивирањем бројлерске производње дошло је до значајног повећања
преваленције различитих патолошких стања чија основна етиологија почива у
самом технолошком процесу производње. Појава контактног дерматитиса на
плантарној кожи екстремитета („foot pad dermatitis“), затим на скочном зглобу
(„hock burns“) и на грудима („breast burns“) данас представља врло раширену
појаву у интензивној производњи, наносећи значајне економске губитке услед
смањеног прираста и одбацивања трупова на линији клања. Поред интензивирања
бројлерске производње значајан утицај на њихову појаву имају исхрана, услови и
начин држања (врста простирке) и генетска предиспозиција. Најзначајнији
фактор ризика за настанак лезија је влажна простирка посебно у комбинацији са
штетним дејством амонијака. Појава лезија има веома велики значај као
индикатор добробити имајући у виду да постоје претпоставке да су установљене
лезије врло болне и да често онемогућавају нормално кретање пилића.
Макроскопским прегледом лезија на плантарној површини уочавају се
инфламаторне лезије праћене некрозом на јастучићима табана и/или на прстима.
Лезије се ређе могу установити на тибиометатарзалним зглобовима. Код благог
облика постоји дисколорација на кожи, хиперкератоза, а у тежим случајевима
јавња се едем, ерозије и улцерације.
Хистопатолошки, благе лезије се карактеришу умереном хиперплазијом и/или
хиперкератозом епидермиса, конгестијом и едемом, уз присуство благог
инфламаторног инфилтрата у форми периваскулитиса и субепителијалне
инфилтрације мононуклеарним ћелијама и мањим бројем гранулоцита. Умерене
суперфицијелне лезије се карактеришу врло израженом хиперплазијом и
хиперкератозом епидермиса, егзоцитозом, појавом пустула и красти, конгестијом
и инфилтрацијом дермиса мононуклеарним и гранулоцитиним инфилтратом.
Тешке, дубоке лезије се карактеришу некрозом епидермиса који је замењен
некротичним гнојним садржајем, појавом гранулационог ткива, израженом
инфламаторном инфилтрацијом (периваскулитис и субепителна инфилтрација)
доминантно хетерофилним гранулоцитима и мононуклеарним ћелијама.
Имајући у виду да је појава контактног дерматитиса све учесталија појава како на
фармама бројлерских пилића тако и на кланици приликом прегледа трупова на
линија клања неопходно је имплементирати програм контроле овог патолошког
стања и на тај начин побољшати добробит и обезбедити производњу квалитетног
и безбедног пилећег меса., The intensification of broiler production has led to a significant increase in the
prevalence of various pathological conditions, whose basic etiology lies in the
production process itself. The occurrence of contact dermatitis on the plantar skin of
extremities ("foot pad dermatitis"), then on the hock joint ("hock burns"), and on the
breasts ("breast burns") is today a very widespread phenomenon in intensive production,
causing significant economic losses due to reduced growth and carcass rejection on the
slaughter line. In addition to the intensification of broiler production, significant factors
influencing their occurrence are nutrition, housing conditions (type of bedding), and
genetic predisposition. The most significant risk factor for lesion formation is wet
bedding, especially in combination with the harmful effects of ammonia. The
occurrence of lesions is very important as an indicator of welfare, considering that there
are assumptions that established lesions are very painful and often prevent normal
chicken movement. Macroscopic examination of lesions on the plantar surface reveals
inflammatory lesions accompanied by necrosis on the foot pads and/or toes. Lesions are
less commonly found on the tibiotarsal joints. In mild forms, discoloration of the skin,
hyperkeratosis, and in severe cases, edema, erosions, and ulcerations occur.
Histopathologically, mild lesions are characterized by moderate epidermal hyperplasia
and/or hyperkeratosis, congestion and edema, with the presence of mild inflammatory
infiltrate in the form of perivasculitis and subepithelial infiltration of mononuclear cells
and a small number of granulocytes. Moderate superficial lesions are characterized by
very pronounced epidermal hyperplasia and hyperkeratosis, exocytosis, appearance of
pustules and crusts, congestion, and infiltration of the dermis with mononuclear and
granulocytic infiltrate. Severe, deep lesions are characterized by epidermal necrosis
replaced by necrotic purulent content, appearance of granulation tissue, pronounced
inflammatory infiltration (perivasculitis and subepithelial infiltration) predominantly
with heterophilic granulocytes and mononuclear cells. Considering that the occurrence
of contact dermatitis is becoming more frequent both on broiler farms and in
slaughterhouses during carcass examination on the slaughter line, it is necessary to
implement a control program for this pathological condition",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.",
title = "Kontaktni pododermatitis kod brojlerskih pilića – patološki aspekt -, Contact pododermatitis in broiler chickens – pathological aspect –",
pages = "147-146"
}

Kureljušić, B., Vićić, I., Bradić, M., Maletić, J., Jezdimirović, N., Savić, B., Milovanović, B.,& Karabasil, N.. (2024). Kontaktni pododermatitis kod brojlerskih pilića – patološki aspekt -. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 146-147.

Kureljušić B, Vićić I, Bradić M, Maletić J, Jezdimirović N, Savić B, Milovanović B, Karabasil N. Kontaktni pododermatitis kod brojlerskih pilića – patološki aspekt -. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.. 2024;:146-147..

Kureljušić, Branislav, Vićić, Ivan, Bradić, Milijana, Maletić, Jelena, Jezdimirović, Nemanja, Savić, Božidar, Milovanović, Bojan, Karabasil, Neđeljko, "Kontaktni pododermatitis kod brojlerskih pilića – patološki aspekt -" in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god. (2024):146-147.

TY  - CONF
AU  - Vasić, Ana
AU  - Kureljušić, Jasna
AU  - Rokvić, Nikola
AU  - Glišić, Dimitrije
AU  - Milovanović, Bojan
AU  - Vasilev, Saša
AU  - Kureljušić, Branislav
AU  - Paunović, Milan
PY  - 2024
UR  - https://reponivs.nivs.rs/handle/123456789/949
AB  - Трихинелоза је широм света присутна зооноза која се преноси конзумирањем
сировог или неправилно термички обрађеног меса или производа од меса
дивљих и домаћих животиња заражених ларвама нематода рода Trichinella.
Дивљи месоједи имају значајну епидемиолошку улогу у силватичном циклусу у
Европи, најчешће ширење Trichinella spp. међу животињама је путем исхране
кроз конзумацију меса одбаченог приликом лова и ингестије лешева који у
мишићном ткиву садрже ларве трихинела.
Од четири врсте Trichinella присутне у Европи (T. spiralis, T. britovi, T. nativa и
T. pseudospiralis), у Србији је присуство Т. spiralis и Т. britovi потврђено код
дивљих месоједа. Иако су статус и трендови популација дивљих карнивора у
Србији стабилни, енормна експанзија популације шакала (Canis aureus) током
последњих 40 година, донела је нове изазове у контроли Trichinella spp.
Циљ нашег истраживања је био да стекнемо увид у преваленцију Trichinella spp.
у популацији дивљих месоједа ( шакали (Canis aureus), лисице (Vulpas vulpes),
дивље мачке (Felis silvaticus) и јазавци (Meles meles)) који су изловљени на три
локалитета који припадају Срему, Граду Београду и Браничевском региону и да
идентификујемо врсте дивљих. месоједа које учествују у природном циклусу
преношења Trichinella spp. у Србији.
Укупно 122 узорка мускулатуре језика животиња одстрељених током 2022. и
2023. године испитано је појединачно коришћењем метода вештачке дигестије.
Тестирано је 67 узорака пореклом од шакала, 43 узорка од лисице, 10 узорака од
јазаваца и 2 од дивљих мачака. Резултати су показали да је 26 (21,14 %) било
позитивно на присуство ларви Trichinella spp. Међу позитивним узорцима је
највише било пореклом од шакала 18 (69,23 %), 7 (26,92 %) узорака пореклом од
лисице, 1 (3,85 %) дивљих мачака и 0 позитивних јазаваца.
Шакали, лисице и дивље мачке укључени су у циклус преноса Trichinella spp. на
основу наших резултата. Молекуларно одређивање врсте трихинеле је
неопходно како би се боље разумели начини преноса и како спречити ширење
дивљих на домаће животиње.
AB  - Trichinellosis is a worldwide zoonosis acquired by consuming raw or improperly
cooked meat or meat-derived products from wild and domestic animals infected with
larvae of nematode worms of the genus Trichinella. Wild carnivores have major
epidemiological role in the sylvatic cycle in Europe, mostly spreading Trichinella spp.
within animals through carnivorous activities and by their scavenger behaviour.
From four Trichinella species present in Europe (namely T. spiralis, T. britovi, T.
nativa and T. pseudospiralis), in Serbia the presence of T. spiralis and T. britovi is
confirmed in wild carnivores. Although the status and trends of the populations of
wild carnivores in Serbia are stable, enormous expansion of Golden jackal (Canis
aureus) population during last 40 years has brought new challenge in the control of
Trichinella spp.
The aim of our research was to access the prevalence of Trichinella spp. in population
of wild carnivores (Golden jackal (Canis aureus), fox (Vulpes vulpes), wild cat (Felis
silvestris) and badger (Meles meles) in three districts in Serbia (Braničevski, Sremski
and City of Belgrade) and to identify wild carnivore species taking part of the natural
transmission cycle of Trichinella spp. in Serbia.
A total of 122 tongue muscle tissue samples of animals hunted down during 2022 and
2023 were examined using artificial digestion method. We tested 67 samples from
jackal, 43 from foxes, 10 from badgers i 2 from wild cats. The results showed that 26
(21.14 %) were positive for the presence of Trichinella spp. larvae, among them 18
(69.23 %) jackals, 7 (26.92 %) foxes, one (3.85 %) wild cat, while none was positive
from badgers.
Jackals, foxes and wild cats are involved in Trichinella spp. transmission cycle.
Further Trichinella species typing is necessary to better understand modes of
transmission and how to prevent spreading from wild to domestic animals.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
T1  - Trihineloza kod divljih mesojeda i njihov značaj u održanju Trichinella spp.
T1  - Trichinelosis in wild carnivores and their significance in natural cycle of Trichinella spp.
EP  - 35
SP  - 34
ER  - 

@conference{
author = "Vasić, Ana and Kureljušić, Jasna and Rokvić, Nikola and Glišić, Dimitrije and Milovanović, Bojan and Vasilev, Saša and Kureljušić, Branislav and Paunović, Milan",
year = "2024",
abstract = "Трихинелоза је широм света присутна зооноза која се преноси конзумирањем
сировог или неправилно термички обрађеног меса или производа од меса
дивљих и домаћих животиња заражених ларвама нематода рода Trichinella.
Дивљи месоједи имају значајну епидемиолошку улогу у силватичном циклусу у
Европи, најчешће ширење Trichinella spp. међу животињама је путем исхране
кроз конзумацију меса одбаченог приликом лова и ингестије лешева који у
мишићном ткиву садрже ларве трихинела.
Од четири врсте Trichinella присутне у Европи (T. spiralis, T. britovi, T. nativa и
T. pseudospiralis), у Србији је присуство Т. spiralis и Т. britovi потврђено код
дивљих месоједа. Иако су статус и трендови популација дивљих карнивора у
Србији стабилни, енормна експанзија популације шакала (Canis aureus) током
последњих 40 година, донела је нове изазове у контроли Trichinella spp.
Циљ нашег истраживања је био да стекнемо увид у преваленцију Trichinella spp.
у популацији дивљих месоједа ( шакали (Canis aureus), лисице (Vulpas vulpes),
дивље мачке (Felis silvaticus) и јазавци (Meles meles)) који су изловљени на три
локалитета који припадају Срему, Граду Београду и Браничевском региону и да
идентификујемо врсте дивљих. месоједа које учествују у природном циклусу
преношења Trichinella spp. у Србији.
Укупно 122 узорка мускулатуре језика животиња одстрељених током 2022. и
2023. године испитано је појединачно коришћењем метода вештачке дигестије.
Тестирано је 67 узорака пореклом од шакала, 43 узорка од лисице, 10 узорака од
јазаваца и 2 од дивљих мачака. Резултати су показали да је 26 (21,14 %) било
позитивно на присуство ларви Trichinella spp. Међу позитивним узорцима је
највише било пореклом од шакала 18 (69,23 %), 7 (26,92 %) узорака пореклом од
лисице, 1 (3,85 %) дивљих мачака и 0 позитивних јазаваца.
Шакали, лисице и дивље мачке укључени су у циклус преноса Trichinella spp. на
основу наших резултата. Молекуларно одређивање врсте трихинеле је
неопходно како би се боље разумели начини преноса и како спречити ширење
дивљих на домаће животиње., Trichinellosis is a worldwide zoonosis acquired by consuming raw or improperly
cooked meat or meat-derived products from wild and domestic animals infected with
larvae of nematode worms of the genus Trichinella. Wild carnivores have major
epidemiological role in the sylvatic cycle in Europe, mostly spreading Trichinella spp.
within animals through carnivorous activities and by their scavenger behaviour.
From four Trichinella species present in Europe (namely T. spiralis, T. britovi, T.
nativa and T. pseudospiralis), in Serbia the presence of T. spiralis and T. britovi is
confirmed in wild carnivores. Although the status and trends of the populations of
wild carnivores in Serbia are stable, enormous expansion of Golden jackal (Canis
aureus) population during last 40 years has brought new challenge in the control of
Trichinella spp.
The aim of our research was to access the prevalence of Trichinella spp. in population
of wild carnivores (Golden jackal (Canis aureus), fox (Vulpes vulpes), wild cat (Felis
silvestris) and badger (Meles meles) in three districts in Serbia (Braničevski, Sremski
and City of Belgrade) and to identify wild carnivore species taking part of the natural
transmission cycle of Trichinella spp. in Serbia.
A total of 122 tongue muscle tissue samples of animals hunted down during 2022 and
2023 were examined using artificial digestion method. We tested 67 samples from
jackal, 43 from foxes, 10 from badgers i 2 from wild cats. The results showed that 26
(21.14 %) were positive for the presence of Trichinella spp. larvae, among them 18
(69.23 %) jackals, 7 (26.92 %) foxes, one (3.85 %) wild cat, while none was positive
from badgers.
Jackals, foxes and wild cats are involved in Trichinella spp. transmission cycle.
Further Trichinella species typing is necessary to better understand modes of
transmission and how to prevent spreading from wild to domestic animals.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.",
title = "Trihineloza kod divljih mesojeda i njihov značaj u održanju Trichinella spp., Trichinelosis in wild carnivores and their significance in natural cycle of Trichinella spp.",
pages = "35-34"
}

Vasić, A., Kureljušić, J., Rokvić, N., Glišić, D., Milovanović, B., Vasilev, S., Kureljušić, B.,& Paunović, M.. (2024). Trihineloza kod divljih mesojeda i njihov značaj u održanju Trichinella spp.. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 34-35.

Vasić A, Kureljušić J, Rokvić N, Glišić D, Milovanović B, Vasilev S, Kureljušić B, Paunović M. Trihineloza kod divljih mesojeda i njihov značaj u održanju Trichinella spp.. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.. 2024;:34-35..

Vasić, Ana, Kureljušić, Jasna, Rokvić, Nikola, Glišić, Dimitrije, Milovanović, Bojan, Vasilev, Saša, Kureljušić, Branislav, Paunović, Milan, "Trihineloza kod divljih mesojeda i njihov značaj u održanju Trichinella spp." in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god. (2024):34-35.

TY  - CONF
AU  - Glišić, Dimitrije
AU  - Zurovac Sapundžić, Zorana
AU  - Šolaja, Sofija
AU  - Kureljušić, Branislav
AU  - Vasković, Nikola
AU  - Đorđević, Milutin
AU  - Mirčeta, Jovan
AU  - Milićević, Vesna
PY  - 2024
UR  - https://reponivs.nivs.rs/handle/123456789/950
AB  - Значај дивљих животиња у ширењу и одржавању заразних болести у
ветеринарској медицини је често занемарен, посебно њихов утицај на
отежавање искорењивања болести код домаћих животиња, истичући потребу за
темељним истраживањем. У овој студији смо хтели да одредимо
серопреваленцију и просторну дистрибуцију одабраних заразних болести међу
популацијама дивљих преживара и то: обичног јелена, јелена лопатара, срне и
муфлона у Србији. Анализирано је 259 узорака серума дивљих преживара.
Узорци су тестирани на присуство антитела на низ патогена, укључујући вирус
плавог језика, Шмаленберг вирус, вирус говеђе дијареје/бордерске болести,
Capripox вирус, вирус Западног Нила, херпес вирус 1 говеда, Кју грознице,
Brucella spp. и Leptospira spp. Присуство антитела на Capripox вирус и
Leptospira spp. није детектовано, што сугерише њихово одсуство у проучаваној
популацији. Код једног узорка детектовано је присуство антитела на вирус
говеђе дијареје/бордерске болести са 98,8% поузданости, што имплицира да је
преваленција ове болести врло ниска у популацији и да је могућност за
ереадикацију сасвим извесна. Надаље, антитела против Brucella spp. и Кју
грознице детектована су код неколико животиња, тачније код једне, односно
три јединке, док су антитела на херпес вирус 1 говеда пронађена у 20,85%
узорака. Серопреваленција за векторске болести је износила 20,5% за
Шмаленберг вирус, 34,3% за грозницу Западног Нила и 38,6% за болест плавог
језика. Ови резултати наглашавају сложене факторе које утичу на здравље
дивљих животиња, укључујући преваленцију болести код домаћих животиња,
карактеристике патогена, услове околине и утицај вектора. Стога је неопходно
размотрити дивље животиње не само као фактор ризика већ као кључан извор
информација за разумевање преваленције болести и механизме њиховог
ширења.
AB  - The often-overlooked importance of wildlife in spreading and maintaining animals'
infectious diseases, especially their impact on complicating the eradication of diseases
in domestic animals, highlights the need for thorough research. In this study, we
wanted to ascertain the seroprevalence and spatial distribution of selected infectious
diseases among wild ruminant populations in Serbia. An analysis was performed
testing 259 wild ruminant sera samples, for the presence of antibodies to certain
pathogens, including bluetongue virus, Schmallenberg virus, Bovine viral
diarrhoea/border disease virus, Capripox virus, West Nile fever virus, Bovine herpes
virus-1, Coxiella burnetii, Brucella spp., and Leptospira spp. Antibodies against
Capripox virus and Leptospira spp., were not detected, suggesting their absence in the
studied population. Antibodies against Bovine viral diarrhoea/border disease virus
were detected in only one sample with 98.8% confidence, implying a minimal
presence of Bovine viral diarrhoea and potentially indicating a status of near
eradication within the examined population. Furthermore, antibodies against Brucella
spp. and Coxiella burnetii were detected only in a few animals, one and three animals,
respectively, while Bovine herpes virus-1 antibodies were found in 20.85% of the
samples. Seroprevalence for vector-borne diseases was recorded at 20.5% for
Schmallenberg disease, 34.3% for West Nile fever, and 38.6% for Bluetongue. These
results elucidate the complex interdependencies influencing the health of wildlife,
which include the prevalence of diseases in domestic animals, pathogen traits,
environmental conditions, and vector capabilities. Therefore, it is imperative to
consider wildlife not solely as a vector of risk, but as a pivotal source of information
for the understanding of disease prevalence and the mechanisms of its spread.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
T1  - Seroprevalencija specifičnih zaraznih bolesti kod divljih preživara u Srbiji
T1  - Seroprevalence analysis of specific infectious diseases in wild ruminants across Serbia
EP  - 77
SP  - 76
ER  - 

@conference{
author = "Glišić, Dimitrije and Zurovac Sapundžić, Zorana and Šolaja, Sofija and Kureljušić, Branislav and Vasković, Nikola and Đorđević, Milutin and Mirčeta, Jovan and Milićević, Vesna",
year = "2024",
abstract = "Значај дивљих животиња у ширењу и одржавању заразних болести у
ветеринарској медицини је често занемарен, посебно њихов утицај на
отежавање искорењивања болести код домаћих животиња, истичући потребу за
темељним истраживањем. У овој студији смо хтели да одредимо
серопреваленцију и просторну дистрибуцију одабраних заразних болести међу
популацијама дивљих преживара и то: обичног јелена, јелена лопатара, срне и
муфлона у Србији. Анализирано је 259 узорака серума дивљих преживара.
Узорци су тестирани на присуство антитела на низ патогена, укључујући вирус
плавог језика, Шмаленберг вирус, вирус говеђе дијареје/бордерске болести,
Capripox вирус, вирус Западног Нила, херпес вирус 1 говеда, Кју грознице,
Brucella spp. и Leptospira spp. Присуство антитела на Capripox вирус и
Leptospira spp. није детектовано, што сугерише њихово одсуство у проучаваној
популацији. Код једног узорка детектовано је присуство антитела на вирус
говеђе дијареје/бордерске болести са 98,8% поузданости, што имплицира да је
преваленција ове болести врло ниска у популацији и да је могућност за
ереадикацију сасвим извесна. Надаље, антитела против Brucella spp. и Кју
грознице детектована су код неколико животиња, тачније код једне, односно
три јединке, док су антитела на херпес вирус 1 говеда пронађена у 20,85%
узорака. Серопреваленција за векторске болести је износила 20,5% за
Шмаленберг вирус, 34,3% за грозницу Западног Нила и 38,6% за болест плавог
језика. Ови резултати наглашавају сложене факторе које утичу на здравље
дивљих животиња, укључујући преваленцију болести код домаћих животиња,
карактеристике патогена, услове околине и утицај вектора. Стога је неопходно
размотрити дивље животиње не само као фактор ризика већ као кључан извор
информација за разумевање преваленције болести и механизме њиховог
ширења., The often-overlooked importance of wildlife in spreading and maintaining animals'
infectious diseases, especially their impact on complicating the eradication of diseases
in domestic animals, highlights the need for thorough research. In this study, we
wanted to ascertain the seroprevalence and spatial distribution of selected infectious
diseases among wild ruminant populations in Serbia. An analysis was performed
testing 259 wild ruminant sera samples, for the presence of antibodies to certain
pathogens, including bluetongue virus, Schmallenberg virus, Bovine viral
diarrhoea/border disease virus, Capripox virus, West Nile fever virus, Bovine herpes
virus-1, Coxiella burnetii, Brucella spp., and Leptospira spp. Antibodies against
Capripox virus and Leptospira spp., were not detected, suggesting their absence in the
studied population. Antibodies against Bovine viral diarrhoea/border disease virus
were detected in only one sample with 98.8% confidence, implying a minimal
presence of Bovine viral diarrhoea and potentially indicating a status of near
eradication within the examined population. Furthermore, antibodies against Brucella
spp. and Coxiella burnetii were detected only in a few animals, one and three animals,
respectively, while Bovine herpes virus-1 antibodies were found in 20.85% of the
samples. Seroprevalence for vector-borne diseases was recorded at 20.5% for
Schmallenberg disease, 34.3% for West Nile fever, and 38.6% for Bluetongue. These
results elucidate the complex interdependencies influencing the health of wildlife,
which include the prevalence of diseases in domestic animals, pathogen traits,
environmental conditions, and vector capabilities. Therefore, it is imperative to
consider wildlife not solely as a vector of risk, but as a pivotal source of information
for the understanding of disease prevalence and the mechanisms of its spread.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.",
title = "Seroprevalencija specifičnih zaraznih bolesti kod divljih preživara u Srbiji, Seroprevalence analysis of specific infectious diseases in wild ruminants across Serbia",
pages = "77-76"
}

Glišić, D., Zurovac Sapundžić, Z., Šolaja, S., Kureljušić, B., Vasković, N., Đorđević, M., Mirčeta, J.,& Milićević, V.. (2024). Seroprevalencija specifičnih zaraznih bolesti kod divljih preživara u Srbiji. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 76-77.

Glišić D, Zurovac Sapundžić Z, Šolaja S, Kureljušić B, Vasković N, Đorđević M, Mirčeta J, Milićević V. Seroprevalencija specifičnih zaraznih bolesti kod divljih preživara u Srbiji. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.. 2024;:76-77..

Glišić, Dimitrije, Zurovac Sapundžić, Zorana, Šolaja, Sofija, Kureljušić, Branislav, Vasković, Nikola, Đorđević, Milutin, Mirčeta, Jovan, Milićević, Vesna, "Seroprevalencija specifičnih zaraznih bolesti kod divljih preživara u Srbiji" in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god. (2024):76-77.

TY  - CONF
AU  - Šolaja, Sofija
AU  - Glišić, Dimitrije
AU  - Zurovac Sapundžić, Zorana
AU  - Milovanović, Bojan
AU  - Maletić, Jelena
AU  - Jezdimirović, Nemanja
AU  - Kureljušić, Branislav
AU  - Milićević, Vesna
PY  - 2024
UR  - https://reponivs.nivs.rs/handle/123456789/954
AB  - Инфективне болести представљају један од главних проблема у интензивном узгоју свиња, упркос технолошком напретку и развоју биосигурносних мера. Вирусна респираторна обољења која имају највећи економски и здравствени значај у свињарству су репродуктивни и респираторни синдром свиња, инфлуенца свиња и Аујецкијева болест. Сеоска газдинства екстензивног типа, и даље предcтављају најчешћи начин узгоја свиња у Србији. Циљ истраживања је био да се утврди серопреваленција респираторних вируса на сеоским газдинствима која представљају извор патогена за комерцијалне фарме. Крвни серуми су узорковани од 222 јединке са 69 газдинстава на којима се налазило до 10 свиња. За детекцију антитела су коришћени комерцијални ЕЛИСА тестови према упутству произвођача. Установљено је 38 (17,1%) серопозитивних јединки на вирус Аујецкијеве болести и 6 (2,7%) на вирус репродуктивног и респираторног синдрома. Антитела на вирус инфлуенце свиња нису установљена ни код једне јединкe. Два газдинства (2,9%) су била серопозитивна на репродуктивни и респираторни синдром са серопреваленцијом у запату од 100%, без присуства антитела на друге тестиране вирусе. Проценат серопозитивних запата на Аујецкијеву болест је био 10,1%, а серопреваленција у запатима је варирала од 33% до 100%. Серонегативних запата на све три болести је било 48 (69,5%). Ови резултати указују да међу свињама на сеоским газдинствима највише циркулише вирус Аујецкијеве болести. Главне катактеристике сеоских газдинстава су неадекватне биосигурносне мере, исхрана помијама и неконтролисан транспорт и контакт са другим врстама животиња. Стога, будући да је здравствени статус оваквих запата непознат, они се сматрају извором високог ризика за комерцијалне фарме. Власници сеоских газдинстава су често запослени на комерцијалним фармама, без обзира на забрану држања свиња, и представљају везу између сеоских газдинстава и комерцијалних фарми. Неопходно је спровести мониторниг здравственог стања, побољшање биосигурносних мера и едукацију власника како би се спречило ширење инфективних обољења са сеоских газдинстава на комерцијалне фарме.
AB  - Infectious diseases represent one of the main challenges in intensive pig farming, despite technological advancements and biosecurity improvement. Viral respiratory diseases with the greatest economic and health significance in pig farming include porcine reproductive and respiratory syndrome (PRRS), swine influenza (SI), and Aujeszky's disease (AD). Small backyard farms continue to be the most common method of pig farming in Serbia. This research aimed to assess the prevalence of respiratory viruses on backyard farms, which serve as a source of pathogens for commercial farms. Serum samples were collected from 222 individuals on 69 farms, each housing up to 10 pigs. Commercial ELISA tests were used for antibody detection according to the manufacturer's instructions. Out of the samples, 38 (17.1%) were seropositive for AD, and 6 (2.7%) for PRRS. No antibodies to the swine influenza virus were detected. Two farms (2.9%) were seropositive for PRRS with a herd-level seroprevalence of 100%, with no antibodies detected for other tested viruses. The percentage of seropositive farms for Aujeszky's disease was 10.1%, with herd-level seroprevalence ranging from 33% to 100%. There were 48 (69.5%) seronegative herds for all three diseases. These results indicate that Aujeszky's disease virus circulates the most among pigs on backyard farms. Main characteristics of backyard farms are low biosecurity, swill feeding, uncontrolled trade and contact with other animal species. Thus, since the health status of backyard farms is unknown, they are considered a high-risk infection source for commercial farms. Owners of backyard farms are often employed on commercial farms, despite regulations, serving as a link between backyard and commercial farms. Implementation of monitoring, improvement of biosecurity measures and education of owners are crucial steps for preventing the transmission of infectious diseases between backyard and commercial farms.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
T1  - Seroprevalencija virusnih respiratornih oboljenja svinja na seoskim gazdinstvima u Srbiji
T1  - Seroprevalence of viral respiratory diseases in swine backyard farms in Serbia
EP  - 107
SP  - 106
ER  - 

@conference{
author = "Šolaja, Sofija and Glišić, Dimitrije and Zurovac Sapundžić, Zorana and Milovanović, Bojan and Maletić, Jelena and Jezdimirović, Nemanja and Kureljušić, Branislav and Milićević, Vesna",
year = "2024",
abstract = "Инфективне болести представљају један од главних проблема у интензивном узгоју свиња, упркос технолошком напретку и развоју биосигурносних мера. Вирусна респираторна обољења која имају највећи економски и здравствени значај у свињарству су репродуктивни и респираторни синдром свиња, инфлуенца свиња и Аујецкијева болест. Сеоска газдинства екстензивног типа, и даље предcтављају најчешћи начин узгоја свиња у Србији. Циљ истраживања је био да се утврди серопреваленција респираторних вируса на сеоским газдинствима која представљају извор патогена за комерцијалне фарме. Крвни серуми су узорковани од 222 јединке са 69 газдинстава на којима се налазило до 10 свиња. За детекцију антитела су коришћени комерцијални ЕЛИСА тестови према упутству произвођача. Установљено је 38 (17,1%) серопозитивних јединки на вирус Аујецкијеве болести и 6 (2,7%) на вирус репродуктивног и респираторног синдрома. Антитела на вирус инфлуенце свиња нису установљена ни код једне јединкe. Два газдинства (2,9%) су била серопозитивна на репродуктивни и респираторни синдром са серопреваленцијом у запату од 100%, без присуства антитела на друге тестиране вирусе. Проценат серопозитивних запата на Аујецкијеву болест је био 10,1%, а серопреваленција у запатима је варирала од 33% до 100%. Серонегативних запата на све три болести је било 48 (69,5%). Ови резултати указују да међу свињама на сеоским газдинствима највише циркулише вирус Аујецкијеве болести. Главне катактеристике сеоских газдинстава су неадекватне биосигурносне мере, исхрана помијама и неконтролисан транспорт и контакт са другим врстама животиња. Стога, будући да је здравствени статус оваквих запата непознат, они се сматрају извором високог ризика за комерцијалне фарме. Власници сеоских газдинстава су често запослени на комерцијалним фармама, без обзира на забрану држања свиња, и представљају везу између сеоских газдинстава и комерцијалних фарми. Неопходно је спровести мониторниг здравственог стања, побољшање биосигурносних мера и едукацију власника како би се спречило ширење инфективних обољења са сеоских газдинстава на комерцијалне фарме., Infectious diseases represent one of the main challenges in intensive pig farming, despite technological advancements and biosecurity improvement. Viral respiratory diseases with the greatest economic and health significance in pig farming include porcine reproductive and respiratory syndrome (PRRS), swine influenza (SI), and Aujeszky's disease (AD). Small backyard farms continue to be the most common method of pig farming in Serbia. This research aimed to assess the prevalence of respiratory viruses on backyard farms, which serve as a source of pathogens for commercial farms. Serum samples were collected from 222 individuals on 69 farms, each housing up to 10 pigs. Commercial ELISA tests were used for antibody detection according to the manufacturer's instructions. Out of the samples, 38 (17.1%) were seropositive for AD, and 6 (2.7%) for PRRS. No antibodies to the swine influenza virus were detected. Two farms (2.9%) were seropositive for PRRS with a herd-level seroprevalence of 100%, with no antibodies detected for other tested viruses. The percentage of seropositive farms for Aujeszky's disease was 10.1%, with herd-level seroprevalence ranging from 33% to 100%. There were 48 (69.5%) seronegative herds for all three diseases. These results indicate that Aujeszky's disease virus circulates the most among pigs on backyard farms. Main characteristics of backyard farms are low biosecurity, swill feeding, uncontrolled trade and contact with other animal species. Thus, since the health status of backyard farms is unknown, they are considered a high-risk infection source for commercial farms. Owners of backyard farms are often employed on commercial farms, despite regulations, serving as a link between backyard and commercial farms. Implementation of monitoring, improvement of biosecurity measures and education of owners are crucial steps for preventing the transmission of infectious diseases between backyard and commercial farms.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.",
title = "Seroprevalencija virusnih respiratornih oboljenja svinja na seoskim gazdinstvima u Srbiji, Seroprevalence of viral respiratory diseases in swine backyard farms in Serbia",
pages = "107-106"
}

Šolaja, S., Glišić, D., Zurovac Sapundžić, Z., Milovanović, B., Maletić, J., Jezdimirović, N., Kureljušić, B.,& Milićević, V.. (2024). Seroprevalencija virusnih respiratornih oboljenja svinja na seoskim gazdinstvima u Srbiji. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 106-107.

Šolaja S, Glišić D, Zurovac Sapundžić Z, Milovanović B, Maletić J, Jezdimirović N, Kureljušić B, Milićević V. Seroprevalencija virusnih respiratornih oboljenja svinja na seoskim gazdinstvima u Srbiji. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.. 2024;:106-107..

Šolaja, Sofija, Glišić, Dimitrije, Zurovac Sapundžić, Zorana, Milovanović, Bojan, Maletić, Jelena, Jezdimirović, Nemanja, Kureljušić, Branislav, Milićević, Vesna, "Seroprevalencija virusnih respiratornih oboljenja svinja na seoskim gazdinstvima u Srbiji" in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god. (2024):106-107.

TY  - JOUR
AU  - Zelenović, Mladen
AU  - Marinković, Darko
AU  - Stević, Nataša
AU  - Stanojević, Slavoljub
AU  - Aničić, Milan
AU  - Milićević, Vesna
AU  - Valčić, Olivera
AU  - Radojičić, Sonja
PY  - 2024
UR  - https://reponivs.nivs.rs/handle/123456789/956
AB  - Brucella ovis infects sheep and causes a clinical or subclinical disease characterized by genital lesions and reduced fertility in rams, placentitis and abortions in ewes, and increased perinatal mortality in lambs. As part of this study, analyses were conducted on a sheep farm near Belgrade (Serbia). Of the serological tests, indirect ELISA was performed. A total of 94 blood sera were analyzed, 33 from rams and 61 from ewes. The results showed 23 (69.7%) positive fi ndings in rams and 2 (3.3%) positive fi ndings in ewes, with an overall prevalence of 25.4% bounded by a 95% confi dence interval. Bruce-ladder multiplex PCR and Real time PCR were performed on 19 serologically positive rams and two serologically positive ewes. The results indicate a slightly higher sensitivity of Real time PCR compared to conventional PCR in diagnosing B. ovis from the reproductive tissues of rams. However, the differences in sensitivity between different nucleic acid extraction protocols were not signifi cant. Most rams showed a positive PCR result in only one sample of reproductive tissue, suggesting the recommendation to take multiple samples from each animal. Further research is needed to bring the sensitivity of molecular tests in diagnosing ram epididymitis closer to the sensitivity of serological tests.
AB  - Brucella ovis infi cira ovce i uzrokuje kliničku ili subkliničku bolest koja se karakteriše genitalnim lezijama i smanjenom plodnošću kod ovnova, placentitisom i pobačajima kod ovaca te povećanom neonatalnom smrtnošću kod jaganjaca. U okviru ovog istraživanja, sprovedene su analize na farmi ovaca u blizini Beograda (Srbija). Od seroloških testova, rađena je indirektna ELISA. Ukupno je analizirano 94 krvna seruma, 33 od ovnova i 61 od ovaca. Rezultati su pokazali 23 (69,7%) pozitivna nalaza kod ovnova i 2 (3,3%) pozitivna nalaza kod ovaca, s ukupnom prevalencijom od 25,4% uz 95% interval pouzdanosti. Bruce-ladder multiplex PCR i Real time PCR su rađeni kod 19 serološki pozitivnih ovnova i dve serološki pozitivne ovce. Rezultati pokazuju da je Real time PCR pokazao nešto veću osetljivost u poređenju s konvencionalnim PCR-om u dijagnozi B. ovis iz reproduktivnih tkiva ovnova. Ipak, razlike u osetljivosti između različitih protokola ekstrakcije nukleinske kiseline nisu bile značajne. Većina ovnova je imala pozitivan PCR rezultat samo kod jednog zbirnog uzorka reproduktivnog tkiva, što ukazuje na preporuku uzimanja više uzoraka od svake životinje. Potrebna su dalja istraživanja kako bi se osetljivost molekularnih testova u dijagnozi epididimitisa ovnova približila osetljivosti seroloških testova.
PB  - Belgrade : Faculty of Veterinary Medicine, University of Belgrade
T2  - Acta Veterinaria-Beograd
T1  - Reliability of molecular tests in diagnosing ovine brucellosis caused by Brucella ovis
EP  - 144
IS  - 1
SP  - 133
VL  - 74
DO  - 10.2478/acve-2024-0010
ER  - 

@article{
author = "Zelenović, Mladen and Marinković, Darko and Stević, Nataša and Stanojević, Slavoljub and Aničić, Milan and Milićević, Vesna and Valčić, Olivera and Radojičić, Sonja",
year = "2024",
abstract = "Brucella ovis infects sheep and causes a clinical or subclinical disease characterized by genital lesions and reduced fertility in rams, placentitis and abortions in ewes, and increased perinatal mortality in lambs. As part of this study, analyses were conducted on a sheep farm near Belgrade (Serbia). Of the serological tests, indirect ELISA was performed. A total of 94 blood sera were analyzed, 33 from rams and 61 from ewes. The results showed 23 (69.7%) positive fi ndings in rams and 2 (3.3%) positive fi ndings in ewes, with an overall prevalence of 25.4% bounded by a 95% confi dence interval. Bruce-ladder multiplex PCR and Real time PCR were performed on 19 serologically positive rams and two serologically positive ewes. The results indicate a slightly higher sensitivity of Real time PCR compared to conventional PCR in diagnosing B. ovis from the reproductive tissues of rams. However, the differences in sensitivity between different nucleic acid extraction protocols were not signifi cant. Most rams showed a positive PCR result in only one sample of reproductive tissue, suggesting the recommendation to take multiple samples from each animal. Further research is needed to bring the sensitivity of molecular tests in diagnosing ram epididymitis closer to the sensitivity of serological tests., Brucella ovis infi cira ovce i uzrokuje kliničku ili subkliničku bolest koja se karakteriše genitalnim lezijama i smanjenom plodnošću kod ovnova, placentitisom i pobačajima kod ovaca te povećanom neonatalnom smrtnošću kod jaganjaca. U okviru ovog istraživanja, sprovedene su analize na farmi ovaca u blizini Beograda (Srbija). Od seroloških testova, rađena je indirektna ELISA. Ukupno je analizirano 94 krvna seruma, 33 od ovnova i 61 od ovaca. Rezultati su pokazali 23 (69,7%) pozitivna nalaza kod ovnova i 2 (3,3%) pozitivna nalaza kod ovaca, s ukupnom prevalencijom od 25,4% uz 95% interval pouzdanosti. Bruce-ladder multiplex PCR i Real time PCR su rađeni kod 19 serološki pozitivnih ovnova i dve serološki pozitivne ovce. Rezultati pokazuju da je Real time PCR pokazao nešto veću osetljivost u poređenju s konvencionalnim PCR-om u dijagnozi B. ovis iz reproduktivnih tkiva ovnova. Ipak, razlike u osetljivosti između različitih protokola ekstrakcije nukleinske kiseline nisu bile značajne. Većina ovnova je imala pozitivan PCR rezultat samo kod jednog zbirnog uzorka reproduktivnog tkiva, što ukazuje na preporuku uzimanja više uzoraka od svake životinje. Potrebna su dalja istraživanja kako bi se osetljivost molekularnih testova u dijagnozi epididimitisa ovnova približila osetljivosti seroloških testova.",
publisher = "Belgrade : Faculty of Veterinary Medicine, University of Belgrade",
journal = "Acta Veterinaria-Beograd",
title = "Reliability of molecular tests in diagnosing ovine brucellosis caused by Brucella ovis",
pages = "144-133",
number = "1",
volume = "74",
doi = "10.2478/acve-2024-0010"
}

Zelenović, M., Marinković, D., Stević, N., Stanojević, S., Aničić, M., Milićević, V., Valčić, O.,& Radojičić, S.. (2024). Reliability of molecular tests in diagnosing ovine brucellosis caused by Brucella ovis. in Acta Veterinaria-Beograd
Belgrade : Faculty of Veterinary Medicine, University of Belgrade., 74(1), 133-144.
https://doi.org/10.2478/acve-2024-0010

Zelenović M, Marinković D, Stević N, Stanojević S, Aničić M, Milićević V, Valčić O, Radojičić S. Reliability of molecular tests in diagnosing ovine brucellosis caused by Brucella ovis. in Acta Veterinaria-Beograd. 2024;74(1):133-144.
doi:10.2478/acve-2024-0010 .

Zelenović, Mladen, Marinković, Darko, Stević, Nataša, Stanojević, Slavoljub, Aničić, Milan, Milićević, Vesna, Valčić, Olivera, Radojičić, Sonja, "Reliability of molecular tests in diagnosing ovine brucellosis caused by Brucella ovis" in Acta Veterinaria-Beograd, 74, no. 1 (2024):133-144,
https://doi.org/10.2478/acve-2024-0010 . .

TY  - JOUR
AU  - Ninković, Milan
AU  - Milićević, Vesna
AU  - Radojičić, Sonja
AU  - Bugarski, Dejan
AU  - Stević, Nataša
PY  - 2024
UR  - https://reponivs.nivs.rs/handle/123456789/948
AB  - Mycoplasma bovis (M. bovis) is a significant pathogen responsible for highly transmissible mastitis in cattle globally. It primarily spreads through colostrum, milk, and semen. Cows with persistent infections act as carriers, intermittently releasing the pathogen, making their milk a pivotal factor in infection transmission. Given the limited seroprevalence surveys in Serbia, this study aimed to detect M. bovis presence in bulk tank milk (BTM), determine route shedding, and evaluate infection risks. BTM samples were collected from 115 dairy farms across Serbia, with M. bovis DNA detected in 11 out of the 115 samples by real-time PCR. Additionally, M. bovis was detected in 1.30% of nasal swabs sampled from apparently healthy animals. A univariate analysis of the risk factors associated with M. bovis presence in the BTM samples revealed correlations with factors such as the breed, farm seropositivity, pre-milking and post-milking disinfection practices, farm type, cow population, milk yield, number of cows in the BTM samples, and parity. Seropositive farms exhibited the highest likelihood of M. bovis presence in milk. Moreover, pre- and post-milking disinfection practices and highly productive cows yielding over 8000 L of milk were identified as risk factors for PCR-positive BTM. In a multivariable mixed regression analysis, a risk factor for the presence of M. bovis infection in the BTM sample was the Holstein breed. These findings underscore a relatively high prevalence of M. bovis in BTM within Serbian dairy farms, suggesting a potential risk for M. bovis spreading through milk and oral route of calves’ infection.
PB  - Basel : MDPI
T2  - Pathogens
T1  - Presence of Mycoplasma bovis in Bulk Tank Milk and Associated Risk Factor Analysis in Serbian Dairy Farms
IS  - 4
SP  - 302
VL  - 13
DO  - 10.3390/pathogens13040302
ER  - 

@article{
author = "Ninković, Milan and Milićević, Vesna and Radojičić, Sonja and Bugarski, Dejan and Stević, Nataša",
year = "2024",
abstract = "Mycoplasma bovis (M. bovis) is a significant pathogen responsible for highly transmissible mastitis in cattle globally. It primarily spreads through colostrum, milk, and semen. Cows with persistent infections act as carriers, intermittently releasing the pathogen, making their milk a pivotal factor in infection transmission. Given the limited seroprevalence surveys in Serbia, this study aimed to detect M. bovis presence in bulk tank milk (BTM), determine route shedding, and evaluate infection risks. BTM samples were collected from 115 dairy farms across Serbia, with M. bovis DNA detected in 11 out of the 115 samples by real-time PCR. Additionally, M. bovis was detected in 1.30% of nasal swabs sampled from apparently healthy animals. A univariate analysis of the risk factors associated with M. bovis presence in the BTM samples revealed correlations with factors such as the breed, farm seropositivity, pre-milking and post-milking disinfection practices, farm type, cow population, milk yield, number of cows in the BTM samples, and parity. Seropositive farms exhibited the highest likelihood of M. bovis presence in milk. Moreover, pre- and post-milking disinfection practices and highly productive cows yielding over 8000 L of milk were identified as risk factors for PCR-positive BTM. In a multivariable mixed regression analysis, a risk factor for the presence of M. bovis infection in the BTM sample was the Holstein breed. These findings underscore a relatively high prevalence of M. bovis in BTM within Serbian dairy farms, suggesting a potential risk for M. bovis spreading through milk and oral route of calves’ infection.",
publisher = "Basel : MDPI",
journal = "Pathogens",
title = "Presence of Mycoplasma bovis in Bulk Tank Milk and Associated Risk Factor Analysis in Serbian Dairy Farms",
number = "4",
pages = "302",
volume = "13",
doi = "10.3390/pathogens13040302"
}

Ninković, M., Milićević, V., Radojičić, S., Bugarski, D.,& Stević, N.. (2024). Presence of Mycoplasma bovis in Bulk Tank Milk and Associated Risk Factor Analysis in Serbian Dairy Farms. in Pathogens
Basel : MDPI., 13(4), 302.
https://doi.org/10.3390/pathogens13040302

Ninković M, Milićević V, Radojičić S, Bugarski D, Stević N. Presence of Mycoplasma bovis in Bulk Tank Milk and Associated Risk Factor Analysis in Serbian Dairy Farms. in Pathogens. 2024;13(4):302.
doi:10.3390/pathogens13040302 .

Ninković, Milan, Milićević, Vesna, Radojičić, Sonja, Bugarski, Dejan, Stević, Nataša, "Presence of Mycoplasma bovis in Bulk Tank Milk and Associated Risk Factor Analysis in Serbian Dairy Farms" in Pathogens, 13, no. 4 (2024):302,
https://doi.org/10.3390/pathogens13040302 . .

TY  - JOUR
AU  - Milićević, Vesna
AU  - Zurovac Sapundžić, Zorana
AU  - Glišić, Dimitrije
AU  - Kureljušić, Branislav
AU  - Vasković, Nikola
AU  - Đorđević, Milutin
AU  - Mirčeta, Jovan
PY  - 2024
UR  - https://reponivs.nivs.rs/handle/123456789/907
AB  - The role of wildlife in maintaining infectious diseases in veterinary medicine is often neglected, although the
disease eradication process in domestic animals is continuously affected by the risk of pathogens transmission
from wildlife as a primary source. The main aim of this paper was to estimate the prevalence and distribution of
selected infectious diseases in wild ruminants in Serbia. In total, 259 sera from wild ruminants were tested for
specific antibodies to bluetongue virus, Schmallenberg virus, Bovine viral diarrhea/border disease virus, Capripox
virus, West Nile fever virus, Bovine herpes virus-1, Coxiella burnetii, Brucella spp., and Leptospira spp.
Specific Capripox virus and Leptospira spp. antibodies were not detected in any of the 259 wild ruminant
samples. Although one animal was detected positive for BVDV/BDV specific antibodies, with 99.8% confidence,
the prevalence of BVD within this population could be very low i.e. essentially free from BVD infection. One and
three positive animals were detected for Brucella spp. and Coxiella burnetii antibodies, respectively. Bovine herpes
virus-1 specific antibodies were detected in 20.85% of the samples. The estimated seroprevalence of vector-borne
diseases was 20.5% for Schmallenberg disease, 34.3% for West Nile fever, and 38.6% for Bluetongue.
Considering the reported results, wildlife health status is a result of different factors in complex relation, such
as the presence of disease in domestic animals, disease nature, pathogen characteristics, environmental factors,
presence, and vector competence. Wildlife should be considered not only as a risk but as a source of important
information on disease distribution and its indicators.
PB  - Elsevier
T2  - Research in Veterinary Science
T1  - Cross-sectional serosurvey of selected infectious diseases in wild ruminants in Serbia
SP  - 105183
VL  - 170
DO  - 10.1016/j.rvsc.2024.105183
ER  - 

@article{
author = "Milićević, Vesna and Zurovac Sapundžić, Zorana and Glišić, Dimitrije and Kureljušić, Branislav and Vasković, Nikola and Đorđević, Milutin and Mirčeta, Jovan",
year = "2024",
abstract = "The role of wildlife in maintaining infectious diseases in veterinary medicine is often neglected, although the
disease eradication process in domestic animals is continuously affected by the risk of pathogens transmission
from wildlife as a primary source. The main aim of this paper was to estimate the prevalence and distribution of
selected infectious diseases in wild ruminants in Serbia. In total, 259 sera from wild ruminants were tested for
specific antibodies to bluetongue virus, Schmallenberg virus, Bovine viral diarrhea/border disease virus, Capripox
virus, West Nile fever virus, Bovine herpes virus-1, Coxiella burnetii, Brucella spp., and Leptospira spp.
Specific Capripox virus and Leptospira spp. antibodies were not detected in any of the 259 wild ruminant
samples. Although one animal was detected positive for BVDV/BDV specific antibodies, with 99.8% confidence,
the prevalence of BVD within this population could be very low i.e. essentially free from BVD infection. One and
three positive animals were detected for Brucella spp. and Coxiella burnetii antibodies, respectively. Bovine herpes
virus-1 specific antibodies were detected in 20.85% of the samples. The estimated seroprevalence of vector-borne
diseases was 20.5% for Schmallenberg disease, 34.3% for West Nile fever, and 38.6% for Bluetongue.
Considering the reported results, wildlife health status is a result of different factors in complex relation, such
as the presence of disease in domestic animals, disease nature, pathogen characteristics, environmental factors,
presence, and vector competence. Wildlife should be considered not only as a risk but as a source of important
information on disease distribution and its indicators.",
publisher = "Elsevier",
journal = "Research in Veterinary Science",
title = "Cross-sectional serosurvey of selected infectious diseases in wild ruminants in Serbia",
pages = "105183",
volume = "170",
doi = "10.1016/j.rvsc.2024.105183"
}

Milićević, V., Zurovac Sapundžić, Z., Glišić, D., Kureljušić, B., Vasković, N., Đorđević, M.,& Mirčeta, J.. (2024). Cross-sectional serosurvey of selected infectious diseases in wild ruminants in Serbia. in Research in Veterinary Science
Elsevier., 170, 105183.
https://doi.org/10.1016/j.rvsc.2024.105183

Milićević V, Zurovac Sapundžić Z, Glišić D, Kureljušić B, Vasković N, Đorđević M, Mirčeta J. Cross-sectional serosurvey of selected infectious diseases in wild ruminants in Serbia. in Research in Veterinary Science. 2024;170:105183.
doi:10.1016/j.rvsc.2024.105183 .

Milićević, Vesna, Zurovac Sapundžić, Zorana, Glišić, Dimitrije, Kureljušić, Branislav, Vasković, Nikola, Đorđević, Milutin, Mirčeta, Jovan, "Cross-sectional serosurvey of selected infectious diseases in wild ruminants in Serbia" in Research in Veterinary Science, 170 (2024):105183,
https://doi.org/10.1016/j.rvsc.2024.105183 . .

TY  - CONF
AU  - Tasic, Aleksandra
AU  - Pavlović, Ivan
AU  - Simeunović, Sara
AU  - Plavša, Nada
AU  - Tasić Dimitrov, Marija
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/1004
AB  - Pesticides used for crop protection can have an indirect effect on target and 
non-target organisms and are considered to be one of the main causes of 
insect decline.Insects may come into touch with polluted surfaces, products, 
or eating or drinking from contaminated sources.Pesticide residues collected 
over bee plants thus they accumulate in the larvae and inside the hive. Also, 
the consequence of the accumulation of pesticides in bees can be their 
presence in bee products. Bee exposure pesticide residues can have similar 
long-term effects on their cognitive capacities, their behavior and fitness. 
Bee poisoning is often associated with exposure to pesticides. Bees can be 
exposed to toxic compounds through contaminated flowers, direct contact 
with the poison or contact with residues. Dusting crops during the flowering 
period can increase the risk of pesticide poisoning for bees. Rapeseed plants 
are very attractive to beneficial insects, especially honey bees (Apis 
mellifera L.).
AB  - Pesticidi koji se koriste za zaštitu useva mogu imati indirektan uticaj na ciljne i neciljne organizme i smatraju se jednim od glavnih uzroka opadanja broja insekata.Insekti mogu doći u dodir sa zagađenim površinama, proizvodima ili jesti ili piti iz kontaminiranih izvora.Ostaci pesticida se sakupljaju preko pčelinjih biljaka i tako se akumuliraju u larvama i unutar košnice. Takođe, posledica akomulacije pesticida u pčelama može biti prisustvo i u pčelinjim proizvodima. Izloženost pčela ostacima pesticida može imati slične dugoročne efekte na njihov kognitivni kapaciteti, njihovo ponašanje i kondiciju. Trovanje pčela je često povezano sa izlaganjem pesticidima. Pčele mogu biti izložene toksičnim jedinjenjima kroz kontaminirano cveće, direktan kontakt sa otrovom ili kontakt sa ostacima. Zaprašivanje useva tokom perioda cvetanja može povećati rizik od trovanja pesticidima za pčela. Biljke uljane repice su veoma privlačne za korisne insekte, posebno za medonosne pčele (Apis mellifera L.).
PB  - Beograd : Akademija pčelarstva i apiterapije Srbije
C3  - 3. International symposium on beekeeping, agriculture, apitherapy and nutrition - sustainable agriculture for a healthy future  : 3. Međunarodni simpozijum o pčelarstvu, poljoprivredi, apiterapija i nutricionizmu  - održiva poljoprivreda za zdravu budućnost
T1  - Beekeeping and plant protection
T1  - Pčelarenje i zaštita bilja
EP  - 17
SP  - 14
ER  - 

@conference{
author = "Tasic, Aleksandra and Pavlović, Ivan and Simeunović, Sara and Plavša, Nada and Tasić Dimitrov, Marija",
year = "2023",
abstract = "Pesticides used for crop protection can have an indirect effect on target and 
non-target organisms and are considered to be one of the main causes of 
insect decline.Insects may come into touch with polluted surfaces, products, 
or eating or drinking from contaminated sources.Pesticide residues collected 
over bee plants thus they accumulate in the larvae and inside the hive. Also, 
the consequence of the accumulation of pesticides in bees can be their 
presence in bee products. Bee exposure pesticide residues can have similar 
long-term effects on their cognitive capacities, their behavior and fitness. 
Bee poisoning is often associated with exposure to pesticides. Bees can be 
exposed to toxic compounds through contaminated flowers, direct contact 
with the poison or contact with residues. Dusting crops during the flowering 
period can increase the risk of pesticide poisoning for bees. Rapeseed plants 
are very attractive to beneficial insects, especially honey bees (Apis 
mellifera L.)., Pesticidi koji se koriste za zaštitu useva mogu imati indirektan uticaj na ciljne i neciljne organizme i smatraju se jednim od glavnih uzroka opadanja broja insekata.Insekti mogu doći u dodir sa zagađenim površinama, proizvodima ili jesti ili piti iz kontaminiranih izvora.Ostaci pesticida se sakupljaju preko pčelinjih biljaka i tako se akumuliraju u larvama i unutar košnice. Takođe, posledica akomulacije pesticida u pčelama može biti prisustvo i u pčelinjim proizvodima. Izloženost pčela ostacima pesticida može imati slične dugoročne efekte na njihov kognitivni kapaciteti, njihovo ponašanje i kondiciju. Trovanje pčela je često povezano sa izlaganjem pesticidima. Pčele mogu biti izložene toksičnim jedinjenjima kroz kontaminirano cveće, direktan kontakt sa otrovom ili kontakt sa ostacima. Zaprašivanje useva tokom perioda cvetanja može povećati rizik od trovanja pesticidima za pčela. Biljke uljane repice su veoma privlačne za korisne insekte, posebno za medonosne pčele (Apis mellifera L.).",
publisher = "Beograd : Akademija pčelarstva i apiterapije Srbije",
journal = "3. International symposium on beekeeping, agriculture, apitherapy and nutrition - sustainable agriculture for a healthy future  : 3. Međunarodni simpozijum o pčelarstvu, poljoprivredi, apiterapija i nutricionizmu  - održiva poljoprivreda za zdravu budućnost",
title = "Beekeeping and plant protection, Pčelarenje i zaštita bilja",
pages = "17-14"
}

Tasic, A., Pavlović, I., Simeunović, S., Plavša, N.,& Tasić Dimitrov, M.. (2023). Beekeeping and plant protection. in 3. International symposium on beekeeping, agriculture, apitherapy and nutrition - sustainable agriculture for a healthy future  : 3. Međunarodni simpozijum o pčelarstvu, poljoprivredi, apiterapija i nutricionizmu  - održiva poljoprivreda za zdravu budućnost
Beograd : Akademija pčelarstva i apiterapije Srbije., 14-17.

Tasic A, Pavlović I, Simeunović S, Plavša N, Tasić Dimitrov M. Beekeeping and plant protection. in 3. International symposium on beekeeping, agriculture, apitherapy and nutrition - sustainable agriculture for a healthy future  : 3. Međunarodni simpozijum o pčelarstvu, poljoprivredi, apiterapija i nutricionizmu  - održiva poljoprivreda za zdravu budućnost. 2023;:14-17..

Tasic, Aleksandra, Pavlović, Ivan, Simeunović, Sara, Plavša, Nada, Tasić Dimitrov, Marija, "Beekeeping and plant protection" in 3. International symposium on beekeeping, agriculture, apitherapy and nutrition - sustainable agriculture for a healthy future  : 3. Međunarodni simpozijum o pčelarstvu, poljoprivredi, apiterapija i nutricionizmu  - održiva poljoprivreda za zdravu budućnost (2023):14-17.

TY  - CONF
AU  - Pavlovic, Ivan
AU  - Plavsa, Nada
AU  - Karpetovska Hristova, Vesna
AU  - Mederle, Narcisa
AU  - Tasic, Aleksandra
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/997
AB  - Wax moths are often found in hives in temperate climates and there are two types of wax
moth in Europe. Both moths belong to family Pyralidae. Galleria mellonella, the greater wax
moth or honeycomb moth is found throughout the world. G. mellonella eggs are laid in the
spring, and they have four life stages. Males are able to generate ultrasonic sound pulses,
which, along with pheromones, are used in mating. The lesser wax moth (Achroia grisella) is
a small moth. Because lesser wax moths eat unoccupied honey bee combs, they are
considered pests to bees and beekeepers. However, unoccupied combs can harbor harmful
pathogens that inflict damage to neighboring insects. Wax moths do not attack the bees
directly, but feed on the shed exoskeletons of bee larvae and pollen that is found in dark brood
comb, which was used by the bees to hold the developing bees. Their full development to
adults requires access to used brood comb or brood cell cleanings—these contain protein
essential for the larval development, in the form of brood cocoons. The destruction of the
comb will spill or contaminate stored honey and may kill bee larvae. When honey supers are
stored for the winter in a mild climate, or in heated storage, the wax moth larvae can destroy
portions of the comb, though they will not fully develop. Damaged comb may be scraped out
and replaced by the bees. Wax moth larvae and eggs are killed by freezing, so storage in
unheated sheds or barns in higher latitudes is the only control necessary.
PB  - IKSAD Publishing House
C3  - International Selçuk scientific research congress, 08-09- July 2023, Konya, Turkey
T1  - The importance of wax moths in the pathology of bees
EP  - 183
SP  - 183
ER  - 

@conference{
author = "Pavlovic, Ivan and Plavsa, Nada and Karpetovska Hristova, Vesna and Mederle, Narcisa and Tasic, Aleksandra",
year = "2023",
abstract = "Wax moths are often found in hives in temperate climates and there are two types of wax
moth in Europe. Both moths belong to family Pyralidae. Galleria mellonella, the greater wax
moth or honeycomb moth is found throughout the world. G. mellonella eggs are laid in the
spring, and they have four life stages. Males are able to generate ultrasonic sound pulses,
which, along with pheromones, are used in mating. The lesser wax moth (Achroia grisella) is
a small moth. Because lesser wax moths eat unoccupied honey bee combs, they are
considered pests to bees and beekeepers. However, unoccupied combs can harbor harmful
pathogens that inflict damage to neighboring insects. Wax moths do not attack the bees
directly, but feed on the shed exoskeletons of bee larvae and pollen that is found in dark brood
comb, which was used by the bees to hold the developing bees. Their full development to
adults requires access to used brood comb or brood cell cleanings—these contain protein
essential for the larval development, in the form of brood cocoons. The destruction of the
comb will spill or contaminate stored honey and may kill bee larvae. When honey supers are
stored for the winter in a mild climate, or in heated storage, the wax moth larvae can destroy
portions of the comb, though they will not fully develop. Damaged comb may be scraped out
and replaced by the bees. Wax moth larvae and eggs are killed by freezing, so storage in
unheated sheds or barns in higher latitudes is the only control necessary.",
publisher = "IKSAD Publishing House",
journal = "International Selçuk scientific research congress, 08-09- July 2023, Konya, Turkey",
title = "The importance of wax moths in the pathology of bees",
pages = "183-183"
}

Pavlovic, I., Plavsa, N., Karpetovska Hristova, V., Mederle, N.,& Tasic, A.. (2023). The importance of wax moths in the pathology of bees. in International Selçuk scientific research congress, 08-09- July 2023, Konya, Turkey
IKSAD Publishing House., 183-183.

Pavlovic I, Plavsa N, Karpetovska Hristova V, Mederle N, Tasic A. The importance of wax moths in the pathology of bees. in International Selçuk scientific research congress, 08-09- July 2023, Konya, Turkey. 2023;:183-183..

Pavlovic, Ivan, Plavsa, Nada, Karpetovska Hristova, Vesna, Mederle, Narcisa, Tasic, Aleksandra, "The importance of wax moths in the pathology of bees" in International Selçuk scientific research congress, 08-09- July 2023, Konya, Turkey (2023):183-183.

TY  - CONF
AU  - Pavlovic, Ivan
AU  - Plavsa, Nada
AU  - Tasic, Aleksandra
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/998
AB  - The Kashmir Bee Virus is common globally and is regarded as one of the most virulent
among all viruses that affect honey bees. Originally, this virus was isolated from samples of
adult Apis cerana bees that came to Rothamsted (UK) from Kashmir. One of the things that
make it so different from other viruses, is the fact that it does not cause infection when
developing honey bee ingests it. It also persists in its dormant state in adult and developing
bees, and will never show any clear symptoms. Nevertheless, the virus becomes fatal when
transmitted by Varroa mites, and will affect all forms of the honey bee life cycle. And it
displays no apparent symptoms.
The virus affects both brood and adult bees. Infected adults die within a few days of exposure
to the virus but infected larvae may survive and develop into seemingly unaffected adults.
While Kashmir bee virus infection alone may not be of much significance, it has been
implicated as part of a much larger agricultural issue. The virus can kill colonies even when
there is a moderate level of mite infestation, just like with the Acute Bee Paralysis Virus
(ABPV). The KBV is closely related to the ABPV when analyzed genetically and
serologically. It is believed that both viruses must have originated from the same ancestor and
taken different evolution paths in secluded geographic regions. The viruses can co-infect the
same honey bee colony and affect the same bee, making the situation much worse.
In Serbia, Kashmir bee virus genome was detected in one sample of imported honey. Since,
as of yet, there has been no evidence of KBV circulation in Serbia, after its detection in
imported honey, there is a substantial risk of its introduction and consequently the need for its
surveillance.
PB  - IKSAD Publishing House
C3  - 5. International Antalya Scientific research and Innovative studies congress, July 26-28, 2023, Antalya, Turkey
T1  - Kashmir bee virus
EP  - 176
SP  - 176
ER  - 

@conference{
author = "Pavlovic, Ivan and Plavsa, Nada and Tasic, Aleksandra",
year = "2023",
abstract = "The Kashmir Bee Virus is common globally and is regarded as one of the most virulent
among all viruses that affect honey bees. Originally, this virus was isolated from samples of
adult Apis cerana bees that came to Rothamsted (UK) from Kashmir. One of the things that
make it so different from other viruses, is the fact that it does not cause infection when
developing honey bee ingests it. It also persists in its dormant state in adult and developing
bees, and will never show any clear symptoms. Nevertheless, the virus becomes fatal when
transmitted by Varroa mites, and will affect all forms of the honey bee life cycle. And it
displays no apparent symptoms.
The virus affects both brood and adult bees. Infected adults die within a few days of exposure
to the virus but infected larvae may survive and develop into seemingly unaffected adults.
While Kashmir bee virus infection alone may not be of much significance, it has been
implicated as part of a much larger agricultural issue. The virus can kill colonies even when
there is a moderate level of mite infestation, just like with the Acute Bee Paralysis Virus
(ABPV). The KBV is closely related to the ABPV when analyzed genetically and
serologically. It is believed that both viruses must have originated from the same ancestor and
taken different evolution paths in secluded geographic regions. The viruses can co-infect the
same honey bee colony and affect the same bee, making the situation much worse.
In Serbia, Kashmir bee virus genome was detected in one sample of imported honey. Since,
as of yet, there has been no evidence of KBV circulation in Serbia, after its detection in
imported honey, there is a substantial risk of its introduction and consequently the need for its
surveillance.",
publisher = "IKSAD Publishing House",
journal = "5. International Antalya Scientific research and Innovative studies congress, July 26-28, 2023, Antalya, Turkey",
title = "Kashmir bee virus",
pages = "176-176"
}

Pavlovic, I., Plavsa, N.,& Tasic, A.. (2023). Kashmir bee virus. in 5. International Antalya Scientific research and Innovative studies congress, July 26-28, 2023, Antalya, Turkey
IKSAD Publishing House., 176-176.

Pavlovic I, Plavsa N, Tasic A. Kashmir bee virus. in 5. International Antalya Scientific research and Innovative studies congress, July 26-28, 2023, Antalya, Turkey. 2023;:176-176..

Pavlovic, Ivan, Plavsa, Nada, Tasic, Aleksandra, "Kashmir bee virus" in 5. International Antalya Scientific research and Innovative studies congress, July 26-28, 2023, Antalya, Turkey (2023):176-176.

TY  - CONF
AU  - Pavlovic, Ivan
AU  - Plavsa, Nada
AU  - Karpetovska Hristova, Vesna
AU  - Mederle, Narcisa
AU  - Tasic, Aleksandra
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/995
AB  - Chalkbrood disease is a fungal disease caused by Ascosphaera apis. Once in a hive, the spores are
accidentally ingested by the larvae. Larvae that are three-to-four days old are particularly susceptible to
infection. Once the fungal spores are eaten, they pass into the larval gut where they germinate and grow
into adult organisms. If the spores are not eaten, they cannot germinate or infect the brood. After a spore
germinates in the larval gut, it quickly grows a mycelium — an intertwined network of filaments that
behave like roots. Each filament grows out from the main body of the fungus in search of water and
nutrients that are used to fuel the organism and produce more spores. The Ascosphaera apis grows so
many of these filaments (often called hyphae) that the brood cell becomes completely stuffed with them.
The filaments steal all the nutrients from the larval host, killing it in the process. The mycelium grows
so prolifically that it eventually forms a mummy, a hardened mass in the shape of the brood cell. The
color of chalkbrood ranges from white to grey then starts to turn black when the fungus is producing
spores or fruiting bodies and ready to reproduce. This is the most infectious stage of chalkbrood. The
black looking mummies are often what you see on the bottom board, outside on the entrance or in front
of the hive. At this point these mummies can spread spores to other colonies in the area. Infected cells
are most often seen on the outer edges of the brood nest. The cell caps may be intact but flattened, they
may have small holes, or they may be partially removed. If the disease is severe, the remaining brood
may appeared scattered and sparse, due to the removal of so many mummies.
PB  - Iksad Global
C3  - 7th International ICONTECH CONGRESS on Innovative Surveys in Positive Sciences, July 4-5, 2023, Ankara, Turkey
T1  - Chalkbrood disease in honey bees
EP  - 172
SP  - 172
ER  - 

@conference{
author = "Pavlovic, Ivan and Plavsa, Nada and Karpetovska Hristova, Vesna and Mederle, Narcisa and Tasic, Aleksandra",
year = "2023",
abstract = "Chalkbrood disease is a fungal disease caused by Ascosphaera apis. Once in a hive, the spores are
accidentally ingested by the larvae. Larvae that are three-to-four days old are particularly susceptible to
infection. Once the fungal spores are eaten, they pass into the larval gut where they germinate and grow
into adult organisms. If the spores are not eaten, they cannot germinate or infect the brood. After a spore
germinates in the larval gut, it quickly grows a mycelium — an intertwined network of filaments that
behave like roots. Each filament grows out from the main body of the fungus in search of water and
nutrients that are used to fuel the organism and produce more spores. The Ascosphaera apis grows so
many of these filaments (often called hyphae) that the brood cell becomes completely stuffed with them.
The filaments steal all the nutrients from the larval host, killing it in the process. The mycelium grows
so prolifically that it eventually forms a mummy, a hardened mass in the shape of the brood cell. The
color of chalkbrood ranges from white to grey then starts to turn black when the fungus is producing
spores or fruiting bodies and ready to reproduce. This is the most infectious stage of chalkbrood. The
black looking mummies are often what you see on the bottom board, outside on the entrance or in front
of the hive. At this point these mummies can spread spores to other colonies in the area. Infected cells
are most often seen on the outer edges of the brood nest. The cell caps may be intact but flattened, they
may have small holes, or they may be partially removed. If the disease is severe, the remaining brood
may appeared scattered and sparse, due to the removal of so many mummies.",
publisher = "Iksad Global",
journal = "7th International ICONTECH CONGRESS on Innovative Surveys in Positive Sciences, July 4-5, 2023, Ankara, Turkey",
title = "Chalkbrood disease in honey bees",
pages = "172-172"
}

Pavlovic, I., Plavsa, N., Karpetovska Hristova, V., Mederle, N.,& Tasic, A.. (2023). Chalkbrood disease in honey bees. in 7th International ICONTECH CONGRESS on Innovative Surveys in Positive Sciences, July 4-5, 2023, Ankara, Turkey
Iksad Global., 172-172.

Pavlovic I, Plavsa N, Karpetovska Hristova V, Mederle N, Tasic A. Chalkbrood disease in honey bees. in 7th International ICONTECH CONGRESS on Innovative Surveys in Positive Sciences, July 4-5, 2023, Ankara, Turkey. 2023;:172-172..

Pavlovic, Ivan, Plavsa, Nada, Karpetovska Hristova, Vesna, Mederle, Narcisa, Tasic, Aleksandra, "Chalkbrood disease in honey bees" in 7th International ICONTECH CONGRESS on Innovative Surveys in Positive Sciences, July 4-5, 2023, Ankara, Turkey (2023):172-172.

TY  - CONF
AU  - Pavlovic, Ivan
AU  - Vojinovic, Dragica
AU  - Tasic, Aleksandra
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/990
AB  - Pasture breeding make possible contact within sheep and eggs, larvae stages and intermediate host of parasites.
The study about gastrointestinal helminthes of small ruminants at east part of Serbia was started in March 2015
and finished in October 2017. Eastern Serbia is a mountain-basin region that stretches from the Danube and the
border with Romania in the north to the Zaplanjsko-Lužnica basin and the Ruj mountain in the south, and from
the border with Bulgaria in the east to the Velika valley and part of the South Morava river in the west. Eastern
Serbia is characterized by a mosaic of rocks of all types and geological formations and a "chess relief" with
alternating mountains and valleys. The relief is dominated by ridged mountains, separated by numerous river
valleys and basins. Đerdap is the largest tunnel in Serbia and Europe (96 km). It consists of a system of valleys
and gorges. Eastern Serbia has a diverse climate: parochial in the valleys, moderately continental in the river
valleys and mountainous on the mountains. Summers are moderately warm; winters are quite cold and windy.
This part of Serbia is well known for its rich horticulture and there is a large number of pastures suitable for
growing small and larger ruminants. During examination 832 fecal samples originated from 57 flocks were
examinatied using standard coprological technique. Total of 69 animals we were analyzed by post-mortem
examination. Determination of eggs and adult parasites we performed by morphologycal characteristic. During
our examination parasites infection we occured in 65.31% and we found next parasite species: Teladorsagia
(Ostertagia) circumcincta, Ostertagia trifurcata, Ostertagia ostertagi, Ostertagia occidentalis, Trichostrongylus
axei, Trichostrongylus colubriformis, Trichostrongylus vitrinus, Nematodirus filicoliis, Nematodirus spathiger,
Nematodirus abnormalis, Haemonchus contortus, Chabertia ovina, Oesophagostomum venulosum, Cooperia
curticei, Cooperia oncophora, Cooperia punctata, Marshallagia marshalli, Skrjabinema ovis and Bunostomum
trigonocephalum.
PB  - IKSAD Publications
C3  - 5. International scientific research and innovation congress
T1  - Gastrointerstinal helminths of small ruminants in east Serbia
EP  - 527
SP  - 527
ER  - 

@conference{
author = "Pavlovic, Ivan and Vojinovic, Dragica and Tasic, Aleksandra",
year = "2023",
abstract = "Pasture breeding make possible contact within sheep and eggs, larvae stages and intermediate host of parasites.
The study about gastrointestinal helminthes of small ruminants at east part of Serbia was started in March 2015
and finished in October 2017. Eastern Serbia is a mountain-basin region that stretches from the Danube and the
border with Romania in the north to the Zaplanjsko-Lužnica basin and the Ruj mountain in the south, and from
the border with Bulgaria in the east to the Velika valley and part of the South Morava river in the west. Eastern
Serbia is characterized by a mosaic of rocks of all types and geological formations and a "chess relief" with
alternating mountains and valleys. The relief is dominated by ridged mountains, separated by numerous river
valleys and basins. Đerdap is the largest tunnel in Serbia and Europe (96 km). It consists of a system of valleys
and gorges. Eastern Serbia has a diverse climate: parochial in the valleys, moderately continental in the river
valleys and mountainous on the mountains. Summers are moderately warm; winters are quite cold and windy.
This part of Serbia is well known for its rich horticulture and there is a large number of pastures suitable for
growing small and larger ruminants. During examination 832 fecal samples originated from 57 flocks were
examinatied using standard coprological technique. Total of 69 animals we were analyzed by post-mortem
examination. Determination of eggs and adult parasites we performed by morphologycal characteristic. During
our examination parasites infection we occured in 65.31% and we found next parasite species: Teladorsagia
(Ostertagia) circumcincta, Ostertagia trifurcata, Ostertagia ostertagi, Ostertagia occidentalis, Trichostrongylus
axei, Trichostrongylus colubriformis, Trichostrongylus vitrinus, Nematodirus filicoliis, Nematodirus spathiger,
Nematodirus abnormalis, Haemonchus contortus, Chabertia ovina, Oesophagostomum venulosum, Cooperia
curticei, Cooperia oncophora, Cooperia punctata, Marshallagia marshalli, Skrjabinema ovis and Bunostomum
trigonocephalum.",
publisher = "IKSAD Publications",
journal = "5. International scientific research and innovation congress",
title = "Gastrointerstinal helminths of small ruminants in east Serbia",
pages = "527-527"
}

Pavlovic, I., Vojinovic, D.,& Tasic, A.. (2023). Gastrointerstinal helminths of small ruminants in east Serbia. in 5. International scientific research and innovation congress
IKSAD Publications., 527-527.

Pavlovic I, Vojinovic D, Tasic A. Gastrointerstinal helminths of small ruminants in east Serbia. in 5. International scientific research and innovation congress. 2023;:527-527..

Pavlovic, Ivan, Vojinovic, Dragica, Tasic, Aleksandra, "Gastrointerstinal helminths of small ruminants in east Serbia" in 5. International scientific research and innovation congress (2023):527-527.

TY  - CONF
AU  - Pavlovic, Ivan
AU  - Stevanovic, Milan
AU  - Zdravkovic, Nemanja
AU  - Tasic, Aleksandra
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/988
AB  - Achroia grisella is a small wax moth of the snout moth family (Pyralidae). Their spread was
worldvide except in areas with cold climates. Females deposit their eggs in crevices in or near
bee hives so that a food source will be close to the emerging larvae. Because lesser wax moths
eat unoccupied honey bee combs, they are considered pests to bees and beekeepers. Feeding
occurs only during the larval life stage. Larvae feed on weak bee colonies. Larvae move through
the bee comb and spin silk tunnels. They cover the silk with their frass. Tunneling through
honeycombs not only provides food, but also protects the larvae from the defending worker
bees. The larvae prefer to eat honey bee larvae, pupae, and pollen, but will also feed on honey.
A disorder called bald brood occurs in hives infested by lesser wax moths. When feeding on
the comb, larvae tunnel under capped cells containing honey bee pupae. This movement causes
the caps to become defective. The worker bees will then remove the defective caps. The name
bald brood refers to the remaining uncapped cells that reveal the residing pupa. During research
conducted in apiaries in Serbia, the presence of small wax moth was established in
approximately 1.57% of apiaries. The damage caused by it did not cause the death of colonies,
but caused economic losses in the production of bee products. Although they are present in a
small percentage, it is necessary to control the small wax moth.
PB  - ISPEC Publishing House
C3  - ISPEC 11th International conference on agriculture, animal science and rural development, 03-05.03.2023. Muş, Turkey
T1  - Presence of small wax moth (Achroia grisella) in apiaries in Serbia
EP  - 169
SP  - 169
ER  - 

@conference{
author = "Pavlovic, Ivan and Stevanovic, Milan and Zdravkovic, Nemanja and Tasic, Aleksandra",
year = "2023",
abstract = "Achroia grisella is a small wax moth of the snout moth family (Pyralidae). Their spread was
worldvide except in areas with cold climates. Females deposit their eggs in crevices in or near
bee hives so that a food source will be close to the emerging larvae. Because lesser wax moths
eat unoccupied honey bee combs, they are considered pests to bees and beekeepers. Feeding
occurs only during the larval life stage. Larvae feed on weak bee colonies. Larvae move through
the bee comb and spin silk tunnels. They cover the silk with their frass. Tunneling through
honeycombs not only provides food, but also protects the larvae from the defending worker
bees. The larvae prefer to eat honey bee larvae, pupae, and pollen, but will also feed on honey.
A disorder called bald brood occurs in hives infested by lesser wax moths. When feeding on
the comb, larvae tunnel under capped cells containing honey bee pupae. This movement causes
the caps to become defective. The worker bees will then remove the defective caps. The name
bald brood refers to the remaining uncapped cells that reveal the residing pupa. During research
conducted in apiaries in Serbia, the presence of small wax moth was established in
approximately 1.57% of apiaries. The damage caused by it did not cause the death of colonies,
but caused economic losses in the production of bee products. Although they are present in a
small percentage, it is necessary to control the small wax moth.",
publisher = "ISPEC Publishing House",
journal = "ISPEC 11th International conference on agriculture, animal science and rural development, 03-05.03.2023. Muş, Turkey",
title = "Presence of small wax moth (Achroia grisella) in apiaries in Serbia",
pages = "169-169"
}

Pavlovic, I., Stevanovic, M., Zdravkovic, N.,& Tasic, A.. (2023). Presence of small wax moth (Achroia grisella) in apiaries in Serbia. in ISPEC 11th International conference on agriculture, animal science and rural development, 03-05.03.2023. Muş, Turkey
ISPEC Publishing House., 169-169.

Pavlovic I, Stevanovic M, Zdravkovic N, Tasic A. Presence of small wax moth (Achroia grisella) in apiaries in Serbia. in ISPEC 11th International conference on agriculture, animal science and rural development, 03-05.03.2023. Muş, Turkey. 2023;:169-169..

Pavlovic, Ivan, Stevanovic, Milan, Zdravkovic, Nemanja, Tasic, Aleksandra, "Presence of small wax moth (Achroia grisella) in apiaries in Serbia" in ISPEC 11th International conference on agriculture, animal science and rural development, 03-05.03.2023. Muş, Turkey (2023):169-169.

TY  - CONF
AU  - Pavlovic, Ivan
AU  - Stevanovic, Milan
AU  - Zdravkovic, Nemanja
AU  - Tasic, Aleksandra
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/976
AB  - Galleria mellonella, the greater wax moth or honeycomb moth, is a moth of the family
Pyralidae. G. mellonella is found throughout the world. Males are able to generate ultrasonic
sound pulses, which, along with pheromones, are used in mating. G.mellonella do not attack
the bees directly, but feed on the shed exoskeletons of bee larvae and pollen that is found in
dark brood comb, which was used by the bees to hold the developing bees. The destruction of
the comb will spill or contaminate stored honey and may kill bee larvae. G. mellonella larvae
parasitize the honeybee. Eggs are laid in the cracks and crevices inside the hive, which
minimizes egg detection . Once eggs hatch, they feed on the midrib of the wax comb, the cast
skins of bee larvae, pollen, and small quantities of propolis and honey. Larvae that hatch tunnel
through the honeycombs that contain honeybee larva and their honey stores. The tunnels they
create are lined with silk, which entangles and starves emerging bees, a phenomenon known as
galleriasis. Tunnels also result in massive destruction of the combs. As a result, honey is wasted
as it leaks out when cell caps are eaten. Finally, both G. mellonella adults and larvae can be
vectors for pathogens that can infect honeybees, including the Israeli acute paralysis virus
(IAPV) and the black queen cell virus (BQCV). During research conducted in apiaries in Serbia,
the presence of greater wax moth was established in approximately 2.3% of apiaries. The
damage caused by it did not cause the death of colonies, but caused great economic losses in
the production of bee products. They also weakened colonies so that in 40% of infected apiaries
we found infections with BQCV in addition to greater wax moth.
PB  - ISPEC Publishing House
C3  - 4rd International Cukurova agriculture and veterinary congress
T1  - Presence of Galleria mellonella, The Greater Wax Moth, in Apiaries in Serbia
EP  - 26
SP  - 26
ER  - 

@conference{
author = "Pavlovic, Ivan and Stevanovic, Milan and Zdravkovic, Nemanja and Tasic, Aleksandra",
year = "2023",
abstract = "Galleria mellonella, the greater wax moth or honeycomb moth, is a moth of the family
Pyralidae. G. mellonella is found throughout the world. Males are able to generate ultrasonic
sound pulses, which, along with pheromones, are used in mating. G.mellonella do not attack
the bees directly, but feed on the shed exoskeletons of bee larvae and pollen that is found in
dark brood comb, which was used by the bees to hold the developing bees. The destruction of
the comb will spill or contaminate stored honey and may kill bee larvae. G. mellonella larvae
parasitize the honeybee. Eggs are laid in the cracks and crevices inside the hive, which
minimizes egg detection . Once eggs hatch, they feed on the midrib of the wax comb, the cast
skins of bee larvae, pollen, and small quantities of propolis and honey. Larvae that hatch tunnel
through the honeycombs that contain honeybee larva and their honey stores. The tunnels they
create are lined with silk, which entangles and starves emerging bees, a phenomenon known as
galleriasis. Tunnels also result in massive destruction of the combs. As a result, honey is wasted
as it leaks out when cell caps are eaten. Finally, both G. mellonella adults and larvae can be
vectors for pathogens that can infect honeybees, including the Israeli acute paralysis virus
(IAPV) and the black queen cell virus (BQCV). During research conducted in apiaries in Serbia,
the presence of greater wax moth was established in approximately 2.3% of apiaries. The
damage caused by it did not cause the death of colonies, but caused great economic losses in
the production of bee products. They also weakened colonies so that in 40% of infected apiaries
we found infections with BQCV in addition to greater wax moth.",
publisher = "ISPEC Publishing House",
journal = "4rd International Cukurova agriculture and veterinary congress",
title = "Presence of Galleria mellonella, The Greater Wax Moth, in Apiaries in Serbia",
pages = "26-26"
}

Pavlovic, I., Stevanovic, M., Zdravkovic, N.,& Tasic, A.. (2023). Presence of Galleria mellonella, The Greater Wax Moth, in Apiaries in Serbia. in 4rd International Cukurova agriculture and veterinary congress
ISPEC Publishing House., 26-26.

Pavlovic I, Stevanovic M, Zdravkovic N, Tasic A. Presence of Galleria mellonella, The Greater Wax Moth, in Apiaries in Serbia. in 4rd International Cukurova agriculture and veterinary congress. 2023;:26-26..

Pavlovic, Ivan, Stevanovic, Milan, Zdravkovic, Nemanja, Tasic, Aleksandra, "Presence of Galleria mellonella, The Greater Wax Moth, in Apiaries in Serbia" in 4rd International Cukurova agriculture and veterinary congress (2023):26-26.

TY  - CONF
AU  - Pavlovic, Ivan
AU  - Zdravkovic, Nemanja
AU  - Caro-Petrovic, Violeta
AU  - Bojkovski, Jovan
AU  - Tasic, Aleksandra
AU  - Pavlovic, Marija
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/868
AB  - The spread area of Belgrade has extremely favorable conditions for modern agricultural
production (climate, agricultural land, watercourses, developed processing industry). This
economic branch is of strategic importance for supplying Belgrade with food products, along
with the resources that abound in the wider environment (Vojvodina and Šumadija). There are
numerous villages here, where households keep cattle in small herds or mini-farm, usually in
semi-intensive breeding. During our study performed on 2018 we examined faeces of 190 cattle
from 42 herds and 29 cattle by post-mortem examination. Determination of eggs and adult
parasites performed on their morphological characteristics. The coprological examination
established the presence of gastrointestinal helminth eggs in 39.6% of samples. The majority
of cattle were infected with two and fewer number with three or four parasite species. At postmortem
examination of cattle we found Haemonchus contortus we occured in 57,53%,
Ostertagia ostertagi in 55,63%, Trichonstrongylus axeis in 49.37%, Cooperia oncophora in
32.57%, Ostertagia trifurcata in 29.79%, Oesophagostomum radiatum 21.22%, Toxocara
vitulorum17.52%, Dicocelium dendriticum 15.26%, Paramphistomum ichikawai in 14.21%,
Strongyloides papillosus 11.51%,Moniezia benedeni in 9.47% and Trichuris discolor in 6.52%.
PB  - Edirne : Trakya University
C3  - V. International Agricultural, Biological & Life Science Conference, Edirne, Turkey, 18-20 September 2023
T1  - Gastrointestinal helmints of cattle in semi intensive breeding at Belgrade area
EP  - 1001
SP  - 996
ER  - 

@conference{
author = "Pavlovic, Ivan and Zdravkovic, Nemanja and Caro-Petrovic, Violeta and Bojkovski, Jovan and Tasic, Aleksandra and Pavlovic, Marija",
year = "2023",
abstract = "The spread area of Belgrade has extremely favorable conditions for modern agricultural
production (climate, agricultural land, watercourses, developed processing industry). This
economic branch is of strategic importance for supplying Belgrade with food products, along
with the resources that abound in the wider environment (Vojvodina and Šumadija). There are
numerous villages here, where households keep cattle in small herds or mini-farm, usually in
semi-intensive breeding. During our study performed on 2018 we examined faeces of 190 cattle
from 42 herds and 29 cattle by post-mortem examination. Determination of eggs and adult
parasites performed on their morphological characteristics. The coprological examination
established the presence of gastrointestinal helminth eggs in 39.6% of samples. The majority
of cattle were infected with two and fewer number with three or four parasite species. At postmortem
examination of cattle we found Haemonchus contortus we occured in 57,53%,
Ostertagia ostertagi in 55,63%, Trichonstrongylus axeis in 49.37%, Cooperia oncophora in
32.57%, Ostertagia trifurcata in 29.79%, Oesophagostomum radiatum 21.22%, Toxocara
vitulorum17.52%, Dicocelium dendriticum 15.26%, Paramphistomum ichikawai in 14.21%,
Strongyloides papillosus 11.51%,Moniezia benedeni in 9.47% and Trichuris discolor in 6.52%.",
publisher = "Edirne : Trakya University",
journal = "V. International Agricultural, Biological & Life Science Conference, Edirne, Turkey, 18-20 September 2023",
title = "Gastrointestinal helmints of cattle in semi intensive breeding at Belgrade area",
pages = "1001-996"
}

Pavlovic, I., Zdravkovic, N., Caro-Petrovic, V., Bojkovski, J., Tasic, A.,& Pavlovic, M.. (2023). Gastrointestinal helmints of cattle in semi intensive breeding at Belgrade area. in V. International Agricultural, Biological & Life Science Conference, Edirne, Turkey, 18-20 September 2023
Edirne : Trakya University., 996-1001.

Pavlovic I, Zdravkovic N, Caro-Petrovic V, Bojkovski J, Tasic A, Pavlovic M. Gastrointestinal helmints of cattle in semi intensive breeding at Belgrade area. in V. International Agricultural, Biological & Life Science Conference, Edirne, Turkey, 18-20 September 2023. 2023;:996-1001..

Pavlovic, Ivan, Zdravkovic, Nemanja, Caro-Petrovic, Violeta, Bojkovski, Jovan, Tasic, Aleksandra, Pavlovic, Marija, "Gastrointestinal helmints of cattle in semi intensive breeding at Belgrade area" in V. International Agricultural, Biological & Life Science Conference, Edirne, Turkey, 18-20 September 2023 (2023):996-1001.

TY  - CONF
AU  - Pavlovic, Ivan
AU  - Caro-Petrovic, Violeta
AU  - Zdravkovic, Nemanja
AU  - Tasic, Aleksandra
AU  - Bojkovski, Jovan
AU  - Pavlovic, Marija
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/864
AB  - The method of breeding, which has been established in breeding of small ruminants for
centuries, is acquired through conditions that affect parasitic infections. From these reasoni in
mind, we started with examination of parasitic fauna of sheep and goat at warious pats of
Serbia. In our paper we presented results of examination performed ın hılly and mountainous
part of Serbia. In the relief of this part of Serbia we a series of surfaces stands out, above
which rise low island mountains and wide valleys cut into the surface. Geographical and
climatic conditions make this region rich in pastures suitable for breeding small ruminants.
They are usually kept in small herds by rural households and spend most of the year grazing.
During our research performed in period 2017-2019, total of 769 fecal samples originated
from 71 sheep and goats flocks were collected individual at monthly intervals. A total of 67
animals we were analyzed by post-mortem examination. Determination of parasites eggs we
performed by morphology characteristic We occured same parasite species: Teladorsagia
(Ostertagia) circumcincta, Ostertagia trifurcata, O.ostertagi, O.occidentalis, Nematodirus
filicoliis, N.spathiger, Trichostrongylus colubriformis, T.axei, T.vitrinus, Haemonchus
contortus, Marshallagia marshalli, Cooperia curticei, C.punctata, C.oncophora, Skrjabinema
ovis, Bunostomum trigonocephalum, Oesophagostomum venulosum and Chabertia ovina.
Poliparasitismus and infection were established at all examined animals. The intensity of
infection and polyparasitsm was monitored in relation to the age of sheep and goats. It was
found that in younger animals intensity of infection was lower than that of older animals. The
infective rate of each of these parasites showed that the most of its followed the same general pattern, having a peak in the spring and another in the autumn, separate by a trough during the
hot dry summer period when the infection rate was low.
PB  - IKSAD Publishing House
C3  - 15th International conference on mathematics, engineering, natural and medical sciences, march 16-18, 2023. Buenos Aires, Argentina
T1  - Gastrointestinal helminths of small ruminants in hilly and mountainous part of Serbia
EP  - 46
SP  - 45
ER  - 

@conference{
author = "Pavlovic, Ivan and Caro-Petrovic, Violeta and Zdravkovic, Nemanja and Tasic, Aleksandra and Bojkovski, Jovan and Pavlovic, Marija",
year = "2023",
abstract = "The method of breeding, which has been established in breeding of small ruminants for
centuries, is acquired through conditions that affect parasitic infections. From these reasoni in
mind, we started with examination of parasitic fauna of sheep and goat at warious pats of
Serbia. In our paper we presented results of examination performed ın hılly and mountainous
part of Serbia. In the relief of this part of Serbia we a series of surfaces stands out, above
which rise low island mountains and wide valleys cut into the surface. Geographical and
climatic conditions make this region rich in pastures suitable for breeding small ruminants.
They are usually kept in small herds by rural households and spend most of the year grazing.
During our research performed in period 2017-2019, total of 769 fecal samples originated
from 71 sheep and goats flocks were collected individual at monthly intervals. A total of 67
animals we were analyzed by post-mortem examination. Determination of parasites eggs we
performed by morphology characteristic We occured same parasite species: Teladorsagia
(Ostertagia) circumcincta, Ostertagia trifurcata, O.ostertagi, O.occidentalis, Nematodirus
filicoliis, N.spathiger, Trichostrongylus colubriformis, T.axei, T.vitrinus, Haemonchus
contortus, Marshallagia marshalli, Cooperia curticei, C.punctata, C.oncophora, Skrjabinema
ovis, Bunostomum trigonocephalum, Oesophagostomum venulosum and Chabertia ovina.
Poliparasitismus and infection were established at all examined animals. The intensity of
infection and polyparasitsm was monitored in relation to the age of sheep and goats. It was
found that in younger animals intensity of infection was lower than that of older animals. The
infective rate of each of these parasites showed that the most of its followed the same general pattern, having a peak in the spring and another in the autumn, separate by a trough during the
hot dry summer period when the infection rate was low.",
publisher = "IKSAD Publishing House",
journal = "15th International conference on mathematics, engineering, natural and medical sciences, march 16-18, 2023. Buenos Aires, Argentina",
title = "Gastrointestinal helminths of small ruminants in hilly and mountainous part of Serbia",
pages = "46-45"
}

Pavlovic, I., Caro-Petrovic, V., Zdravkovic, N., Tasic, A., Bojkovski, J.,& Pavlovic, M.. (2023). Gastrointestinal helminths of small ruminants in hilly and mountainous part of Serbia. in 15th International conference on mathematics, engineering, natural and medical sciences, march 16-18, 2023. Buenos Aires, Argentina
IKSAD Publishing House., 45-46.

Pavlovic I, Caro-Petrovic V, Zdravkovic N, Tasic A, Bojkovski J, Pavlovic M. Gastrointestinal helminths of small ruminants in hilly and mountainous part of Serbia. in 15th International conference on mathematics, engineering, natural and medical sciences, march 16-18, 2023. Buenos Aires, Argentina. 2023;:45-46..

Pavlovic, Ivan, Caro-Petrovic, Violeta, Zdravkovic, Nemanja, Tasic, Aleksandra, Bojkovski, Jovan, Pavlovic, Marija, "Gastrointestinal helminths of small ruminants in hilly and mountainous part of Serbia" in 15th International conference on mathematics, engineering, natural and medical sciences, march 16-18, 2023. Buenos Aires, Argentina (2023):45-46.