Dirofilaria sp. and Blood Meal Analysis in Mosquitoes Collected in Vojvodina and Maˇcva, and the First Report of Setaria tundra (Issaitshikoff & Rajewskaya, 1928) in Serbia
Аутори
Šiljegović, SaraMouillaud, Théo
Jiolle, Davy
Petrić, Dušan
Ignjatović-Ćupina, Aleksandra
Vasić, Ana
Paupy, Christophe
Kavran, Mihaela
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Dirofilaria immitis and D. repens are the two most widespread and important species
of mosquito-borne nematodes, posing a significant threat to veterinary health and particularly
affecting canines and felines. While D. immitis causes cardiopulmonary dirofilariasis, D. repens causes
subcutaneous infections in dogs and other carnivores. Despite the extensive knowledge on these
parasites, little is known about their natural vectors in Serbia. The parasite Setaria tundra, known to
infect deer, has not yet been detected in Serbia but has been documented in neighboring countries.
Thus, the aim of this study was to (i) further map out Dirofilaria sp. hotspots in the Vojvodina
Province and detect S. tundra for the first time, (ii) detect positive mosquito species that can provide
insights into how the nematodes spread in Serbia, and (iii) analyze the blood-fed female mosquitoes
of species found to be infected, in order to identify the potential source of parasite infection. A total of...
2902 female mosquitoes were collected across 73 locations during 2021 and 2022. Molecular biology
methods, based on conventional PCR, were used to analyze non-blood-fed (2521 specimens) and
blood-fed (381 specimens) female mosquitos, in order to detect filarial nematode presence and identify
blood-meal sources, respectively. When the parasite genome was detected, the amplicon (cox1 gene,
650 bp fragment) was sent for Sanger sequencing, further confirming the presence of nematodes and
species assignation. D. immitis was detected in three Culex pipiens mosquitoes collected in Zrenjanin
(August 2021) and Glogonj and Svetozar Miletić (both in July 2021). Additionally, Setaria tundra was
detected in Aedes vexans collected in Iđoš (mid-August 2021) and Aedes caspius, which was collected in Mali Iđoš (end of July 2021). This work identifies two new locations where D. immitis occurs
in Vojvodina, and is the first report of S. tundra in Serbian territory. Blood-meal analysis provided
insights into the preferences of mosquitoes that were positive for Dirofilaria sp. and S. tundra.
Кључне речи:
Dirofilaria sp. / Setaria tundra / mosquito surveillance / Cox1 geneИзвор:
Animals, 2024, 14, 1255-Издавач:
- Basel . MDPI
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200117 (Универзитет у Новом Саду, Пољопривредни факултет) (RS-MESTD-inst-2020-200117)
- COST Action CA17108
- Secretariat for Urbanism and Environment Protection of Vojvodina Province (Serbia)
- Loznica Municipality
Институција/група
Naučni institut za veterinarstvo SrbijeTY - JOUR AU - Šiljegović, Sara AU - Mouillaud, Théo AU - Jiolle, Davy AU - Petrić, Dušan AU - Ignjatović-Ćupina, Aleksandra AU - Vasić, Ana AU - Paupy, Christophe AU - Kavran, Mihaela PY - 2024 UR - https://reponivs.nivs.rs/handle/123456789/946 AB - Dirofilaria immitis and D. repens are the two most widespread and important species of mosquito-borne nematodes, posing a significant threat to veterinary health and particularly affecting canines and felines. While D. immitis causes cardiopulmonary dirofilariasis, D. repens causes subcutaneous infections in dogs and other carnivores. Despite the extensive knowledge on these parasites, little is known about their natural vectors in Serbia. The parasite Setaria tundra, known to infect deer, has not yet been detected in Serbia but has been documented in neighboring countries. Thus, the aim of this study was to (i) further map out Dirofilaria sp. hotspots in the Vojvodina Province and detect S. tundra for the first time, (ii) detect positive mosquito species that can provide insights into how the nematodes spread in Serbia, and (iii) analyze the blood-fed female mosquitoes of species found to be infected, in order to identify the potential source of parasite infection. A total of 2902 female mosquitoes were collected across 73 locations during 2021 and 2022. Molecular biology methods, based on conventional PCR, were used to analyze non-blood-fed (2521 specimens) and blood-fed (381 specimens) female mosquitos, in order to detect filarial nematode presence and identify blood-meal sources, respectively. When the parasite genome was detected, the amplicon (cox1 gene, 650 bp fragment) was sent for Sanger sequencing, further confirming the presence of nematodes and species assignation. D. immitis was detected in three Culex pipiens mosquitoes collected in Zrenjanin (August 2021) and Glogonj and Svetozar Miletić (both in July 2021). Additionally, Setaria tundra was detected in Aedes vexans collected in Iđoš (mid-August 2021) and Aedes caspius, which was collected in Mali Iđoš (end of July 2021). This work identifies two new locations where D. immitis occurs in Vojvodina, and is the first report of S. tundra in Serbian territory. Blood-meal analysis provided insights into the preferences of mosquitoes that were positive for Dirofilaria sp. and S. tundra. PB - Basel . MDPI T2 - Animals T1 - Dirofilaria sp. and Blood Meal Analysis in Mosquitoes Collected in Vojvodina and Maˇcva, and the First Report of Setaria tundra (Issaitshikoff & Rajewskaya, 1928) in Serbia SP - 1255 VL - 14 DO - 10.3390/ani14091255 ER -
@article{ author = "Šiljegović, Sara and Mouillaud, Théo and Jiolle, Davy and Petrić, Dušan and Ignjatović-Ćupina, Aleksandra and Vasić, Ana and Paupy, Christophe and Kavran, Mihaela", year = "2024", abstract = "Dirofilaria immitis and D. repens are the two most widespread and important species of mosquito-borne nematodes, posing a significant threat to veterinary health and particularly affecting canines and felines. While D. immitis causes cardiopulmonary dirofilariasis, D. repens causes subcutaneous infections in dogs and other carnivores. Despite the extensive knowledge on these parasites, little is known about their natural vectors in Serbia. The parasite Setaria tundra, known to infect deer, has not yet been detected in Serbia but has been documented in neighboring countries. Thus, the aim of this study was to (i) further map out Dirofilaria sp. hotspots in the Vojvodina Province and detect S. tundra for the first time, (ii) detect positive mosquito species that can provide insights into how the nematodes spread in Serbia, and (iii) analyze the blood-fed female mosquitoes of species found to be infected, in order to identify the potential source of parasite infection. A total of 2902 female mosquitoes were collected across 73 locations during 2021 and 2022. Molecular biology methods, based on conventional PCR, were used to analyze non-blood-fed (2521 specimens) and blood-fed (381 specimens) female mosquitos, in order to detect filarial nematode presence and identify blood-meal sources, respectively. When the parasite genome was detected, the amplicon (cox1 gene, 650 bp fragment) was sent for Sanger sequencing, further confirming the presence of nematodes and species assignation. D. immitis was detected in three Culex pipiens mosquitoes collected in Zrenjanin (August 2021) and Glogonj and Svetozar Miletić (both in July 2021). Additionally, Setaria tundra was detected in Aedes vexans collected in Iđoš (mid-August 2021) and Aedes caspius, which was collected in Mali Iđoš (end of July 2021). This work identifies two new locations where D. immitis occurs in Vojvodina, and is the first report of S. tundra in Serbian territory. Blood-meal analysis provided insights into the preferences of mosquitoes that were positive for Dirofilaria sp. and S. tundra.", publisher = "Basel . MDPI", journal = "Animals", title = "Dirofilaria sp. and Blood Meal Analysis in Mosquitoes Collected in Vojvodina and Maˇcva, and the First Report of Setaria tundra (Issaitshikoff & Rajewskaya, 1928) in Serbia", pages = "1255", volume = "14", doi = "10.3390/ani14091255" }
Šiljegović, S., Mouillaud, T., Jiolle, D., Petrić, D., Ignjatović-Ćupina, A., Vasić, A., Paupy, C.,& Kavran, M.. (2024). Dirofilaria sp. and Blood Meal Analysis in Mosquitoes Collected in Vojvodina and Maˇcva, and the First Report of Setaria tundra (Issaitshikoff & Rajewskaya, 1928) in Serbia. in Animals Basel . MDPI., 14, 1255. https://doi.org/10.3390/ani14091255
Šiljegović S, Mouillaud T, Jiolle D, Petrić D, Ignjatović-Ćupina A, Vasić A, Paupy C, Kavran M. Dirofilaria sp. and Blood Meal Analysis in Mosquitoes Collected in Vojvodina and Maˇcva, and the First Report of Setaria tundra (Issaitshikoff & Rajewskaya, 1928) in Serbia. in Animals. 2024;14:1255. doi:10.3390/ani14091255 .
Šiljegović, Sara, Mouillaud, Théo, Jiolle, Davy, Petrić, Dušan, Ignjatović-Ćupina, Aleksandra, Vasić, Ana, Paupy, Christophe, Kavran, Mihaela, "Dirofilaria sp. and Blood Meal Analysis in Mosquitoes Collected in Vojvodina and Maˇcva, and the First Report of Setaria tundra (Issaitshikoff & Rajewskaya, 1928) in Serbia" in Animals, 14 (2024):1255, https://doi.org/10.3390/ani14091255 . .