TY  - CONF
AU  - Jezdimirović, Nemanja
AU  - Kureljušić, Branislav
AU  - Savić, Božidar
AU  - Milovanović, Bojan
AU  - Glišić, Dimitrije
AU  - Maletić, Jelena
AU  - Šolaja, Sofija
AU  - Milićević, Vesna
PY  - 2024
UR  - https://reponivs.nivs.rs/handle/123456789/952
AB  - Дивље свиње могу бити резервоари различитих бактерија, вируса и паразита и
могу се пренети на домаће свиње па и на људе индиректним или директним
контактом. Порцине цитомегаловирус (ПЦМВ) препознат је као главни
узрочник атрофичног ринитиса код свиња свих старосних категорија.
Међународни комитет за таксономију вируса (ИТЦВ) је порцине
цитомегаловирус сврстао у suid betaherpesvirus 2 (SuBHV2), и припада
подфамилији Betaherpesvirinae и фамилији Herpesviridae, али није сврстан ни у
један род. Генетска истраживања су показала да је ПЦМВ генетски сроднији
хуманим херпес вирусима (ХХВ) 6А, 6Б и 7. Поред тога, доказано је да ПЦМВ
доприноси репродуктивним проблемима код крмача и резултира раним
неонаталним губицима. Инфекција ПЦМВ-ом је раширена широм света и има
високу преваленцију, посебно у земљама са интензивном производњом свиња.
Клинички пролази непримећено због стеченог имунитета запата, и обично се
јавља код млађих категорија животиња. Нажалост, сама болест и инфекција су
углавном занемарени.
Како бисмо открили улогу дивљих свиња у епидемиологији ПЦМВ-а,
користили смо реал-тиме ПЦР тестирање 50 узорака (лимфни чвор, слезина и
бубрег) пореклом од одстрељених дивљих свиња.
Испитивање је потврдило присуство генома ПЦМВ у 4/50 (8%) узорака. Ови
резултати указују на релативно ниску али не и занемарљиву преваленцију
вируса у популацији дивљих свиња, што може да представља значајан ризик и
за популацију домаћих свиња.
AB  - Wild boars can be reservoirs of various bacteria, viruses, and parasites and can be
transmitted to domestic pigs and even humans through indirect or direct contact.
Porcine cytomegalovirus (PCMV) is recognized as the main cause of atrophic rhinitis
in pigs of all age categories. The International Committee on Taxonomy of Viruses
(ICTV) has classified porcine cytomegalovirus into suid betaherpesvirus 2
(SuBHV2), belonging to the subfamily Betaherpesvirinae and family Herpesviridae,
but it is not classified into any genus. Genetic research has shown that PCMV is
genetically closer to human herpes viruses (HHV) 6A, 6B, and 7. Additionally, it has
been proven that PCMV contributes to reproductive problems in sows and results in
early neonatal losses. PCMV infection is widespread worldwide and has a high
prevalence, especially in countries with intensive pig production. Clinically, it often
goes unnoticed due to acquired maternal immunity and usually occurs in younger
animal categories. Unfortunately, the disease itself and the infection are largely
neglected.
To discover the role of wild boars in PCMV epidemiology, we used real-time PCR
testing of 50 samples (lymph nodes, spleen, and kidney) originating from shot wild
boars.
Testing confirmed the presence of PCMV genome in 4 out of 50 (8%) samples. These
results indicate a relatively low but not negligible prevalence of the virus in the wild
boar population, which may pose a significant risk to the domestic pig population.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
T1  - Prva molekularna detekcija porcine citomegalovirusa kod divljih svinja u Srbiji
T1  - First molecular detection of porcine cytomegalovirus in wild boars in Serbia
EP  - 79
SP  - 78
ER  - 

@conference{
author = "Jezdimirović, Nemanja and Kureljušić, Branislav and Savić, Božidar and Milovanović, Bojan and Glišić, Dimitrije and Maletić, Jelena and Šolaja, Sofija and Milićević, Vesna",
year = "2024",
abstract = "Дивље свиње могу бити резервоари различитих бактерија, вируса и паразита и
могу се пренети на домаће свиње па и на људе индиректним или директним
контактом. Порцине цитомегаловирус (ПЦМВ) препознат је као главни
узрочник атрофичног ринитиса код свиња свих старосних категорија.
Међународни комитет за таксономију вируса (ИТЦВ) је порцине
цитомегаловирус сврстао у suid betaherpesvirus 2 (SuBHV2), и припада
подфамилији Betaherpesvirinae и фамилији Herpesviridae, али није сврстан ни у
један род. Генетска истраживања су показала да је ПЦМВ генетски сроднији
хуманим херпес вирусима (ХХВ) 6А, 6Б и 7. Поред тога, доказано је да ПЦМВ
доприноси репродуктивним проблемима код крмача и резултира раним
неонаталним губицима. Инфекција ПЦМВ-ом је раширена широм света и има
високу преваленцију, посебно у земљама са интензивном производњом свиња.
Клинички пролази непримећено због стеченог имунитета запата, и обично се
јавља код млађих категорија животиња. Нажалост, сама болест и инфекција су
углавном занемарени.
Како бисмо открили улогу дивљих свиња у епидемиологији ПЦМВ-а,
користили смо реал-тиме ПЦР тестирање 50 узорака (лимфни чвор, слезина и
бубрег) пореклом од одстрељених дивљих свиња.
Испитивање је потврдило присуство генома ПЦМВ у 4/50 (8%) узорака. Ови
резултати указују на релативно ниску али не и занемарљиву преваленцију
вируса у популацији дивљих свиња, што може да представља значајан ризик и
за популацију домаћих свиња., Wild boars can be reservoirs of various bacteria, viruses, and parasites and can be
transmitted to domestic pigs and even humans through indirect or direct contact.
Porcine cytomegalovirus (PCMV) is recognized as the main cause of atrophic rhinitis
in pigs of all age categories. The International Committee on Taxonomy of Viruses
(ICTV) has classified porcine cytomegalovirus into suid betaherpesvirus 2
(SuBHV2), belonging to the subfamily Betaherpesvirinae and family Herpesviridae,
but it is not classified into any genus. Genetic research has shown that PCMV is
genetically closer to human herpes viruses (HHV) 6A, 6B, and 7. Additionally, it has
been proven that PCMV contributes to reproductive problems in sows and results in
early neonatal losses. PCMV infection is widespread worldwide and has a high
prevalence, especially in countries with intensive pig production. Clinically, it often
goes unnoticed due to acquired maternal immunity and usually occurs in younger
animal categories. Unfortunately, the disease itself and the infection are largely
neglected.
To discover the role of wild boars in PCMV epidemiology, we used real-time PCR
testing of 50 samples (lymph nodes, spleen, and kidney) originating from shot wild
boars.
Testing confirmed the presence of PCMV genome in 4 out of 50 (8%) samples. These
results indicate a relatively low but not negligible prevalence of the virus in the wild
boar population, which may pose a significant risk to the domestic pig population.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.",
title = "Prva molekularna detekcija porcine citomegalovirusa kod divljih svinja u Srbiji, First molecular detection of porcine cytomegalovirus in wild boars in Serbia",
pages = "79-78"
}

Jezdimirović, N., Kureljušić, B., Savić, B., Milovanović, B., Glišić, D., Maletić, J., Šolaja, S.,& Milićević, V.. (2024). Prva molekularna detekcija porcine citomegalovirusa kod divljih svinja u Srbiji. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 78-79.

Jezdimirović N, Kureljušić B, Savić B, Milovanović B, Glišić D, Maletić J, Šolaja S, Milićević V. Prva molekularna detekcija porcine citomegalovirusa kod divljih svinja u Srbiji. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.. 2024;:78-79..

Jezdimirović, Nemanja, Kureljušić, Branislav, Savić, Božidar, Milovanović, Bojan, Glišić, Dimitrije, Maletić, Jelena, Šolaja, Sofija, Milićević, Vesna, "Prva molekularna detekcija porcine citomegalovirusa kod divljih svinja u Srbiji" in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god. (2024):78-79.

TY  - CONF
AU  - Savić, Božidar
AU  - Milićević, Vesna
AU  - Kureljušić, Branislav
AU  - Jezdimirović, Nemanja
AU  - Milovanović, Bojan
AU  - Maksimović Zorić, Jelena
AU  - Stevnčević, Ognjen
PY  - 2024
UR  - https://reponivs.nivs.rs/handle/123456789/953
AB  - Репродуктивни и респираторни синдром свиња (енгл. Porcine Reproductive and Respiratory
Syndrome – PRRS), је контагиозна вирусна афекција свиња која тренутно представља
економски најзначајније инфективно обољење свиња, ендемично у многим земљама у
свету укључујучи и нашу земљу. PRRS је узрокован са вирусом репродуктивног и
респираторног синдрома свиња (енгл. Porcine Reproductive and Respiratory Syndrome Virus
– PRRSV), а с краја 90-их година прошлог века, када је обољење по први пут
дијагностиковано у нашој земљи, постоји релативно ограничен број епидемиолошких
студија овог обољења и молекуларних истраживања овог вируса који циркулишу у
популацији свиња. Описана су два генотипа PRRSV: тип-1 или Европски тип (Lelystad -
прототип) и тип-2 или Амерички тип (VR-2332 – прототип). Од недавно, PRRSV тип-1 и
тип-2 су класификовани као две засебне врсте унутар рода Betaarterivirus и то као
Betaarterivirus suid 1 (PRRSV1) и Betaarterivirus suid 2 (PRRSV2). У оквиру генотипа 1
(PRRSV1) се разликују 4 генетске линије (подтипа) (1, 2, 3 и 4) у које су сврстани изолати
вируса пореклом из различитих географских региона Европе. Унутар подтипова 1 и 3, се
диференцирају кластери 1А-1Г и 3А-3Г док подтип 2 чини јединствена група изолата, без
дефинисаних кластера. У нашој земљи, PRRS је био искључиво узрокован генотипом 1
(PRRSV1), међутим, 2022 године, детектован је и генотип 2 вируса (PRRSV2), на фарми
која је вршила увоз свиња из Европске уније. Анализом генома овог вируса је установљено
да је најсличнији тзв. вакциналним-сојевима (vaccine-like strain), односно да је вирус
највероватније пореклом из вакцине (МLV PRRSV2 вакцина) која је употребљена за
имунизацију свиња против PRRSV2 у земљи извозници. Циљ овог истраживањ је био да
се установи генетски диверзитет изолата PRRSV1 пореклом са фарми свиња из наше земље
у периоду од 2012 до 2022 године. У ту сврху, извршено је секвенцирање 36 ОРФ5 гена
изолата PRRSV1 коришћењем Сангер методе, које су потом поређене и анализиране
заједно са 106 секвенци ОРФ5 гена PRRSV1 из генетских линија 1, 2 и 3, које репрезентују
комплетан спектар генетског диверзитета ових подтипова (секвенце кластера 1А-1Г, 3А-
3Г и линије 2), применом различитих модула са предефинисаним параметарима у оквиру
софтверских пакета CLC – MainWorkbench и Mega X. Прикупљене секвнеце ОРФ5 гена су
депоноване у „NCBI“ бази биоинформатичких података. Филогенетском анализом је
установљено да су сви изолати PRRSV1 детектовани у периооду од 2012 до 2022 године у
нашој земљи груписани у два подтипа, односно подтип 1 и 3. У оквиру линије 1, се налази
7 изолата и то: 2 изолата у кластеру 1А, 2 у кластеру 1Б, 2 у кластеру 1Д и један изолат у
кластеру 1Ф. У оквиру линије 2, 29 секвенци је груписано у кластер 3Ц. Добијени
резултати представљају важне информације за будуће епидемиолошке анализе PRRSV1
инфекције укључујући утврђивање повезаности односно сродности између изолата вируса,
поређење нових секвенци са секвенцама пореклом од вакциналних сојева вируса,
разликовање вакциналних од „дивљих“ сојева вируса и утврђивање „уноса“ нових вируса
у популацију. Оваква епидемиолошка истраживања треба искористити за установљавање
критичних тачака у биосигурносним мерама на конкретним фармама, те имплементирати
одговарајуће промене како би се PRRSV инфекција успешно превенирала и контролисала.
AB  - Porcine Reproductive and Respiratory Syndrome (PRRS) is a contagious viral disease
of swine that currently represents the most economically significant infectious disease
of pigs, endemic in many countries worldwide, including Serbia. PRRS is caused by the
Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). Since the late 1990s,
when the disease was first diagnosed in the Serbia, there have been only few
epidemiological and molecular studies on circulating PPRS-viruses. Two genotypes of
PRRSV have been described: type 1 or European type (prototype Lelystad) and type 2
or American type (prototype VR-2332). Recently, PRRSV type 1 and type 2 have been
classified as two separate species within the genus Betaarterivirus, namely
Betaarterivirus suid 1 (PRRSV1) and Betaarterivirus suid 2 (PRRSV2). Within
genotype 1 (PRRSV1), four genetic lineages (subtypes) (1, 2, 3, and 4) have been
distinguished, in which isolates from different geographic regions of Europe are
classified. Within subtypes 1 and 3, clusters 1A-1G and 3A-3G are further differentiated,
while subtype 2 consists of a unique group of isolates, without defined clusters. In Serbia,
PRRS was exclusively caused by genotype 1 (PRRSV1); however, in 2022, a genotype
2 (PRRSV2) was detected on a farm that imported pigs from the European Union.
Genome analysis of this virus revealed its similarity to vaccine-like strains, indicating
that the virus most likely originated from a vaccine (MLV PRRSV2 vaccine) used for
immunization against PRRSV2 in the exporting country.
The aim of this study was to establish the genetic diversity of PRRSV1 isolates
originating from pig farms in Serbia from 2012 to 2022. For this purpose, sequencing of
36 ORF5 gene of PRRSV1 was performed using the Sanger method, which were then
compared and analyzed together with 106 ORF5 gene sequences of PRRSV1 from
genetic lineages 1, 2, and 3, representing the full range of genetic diversity of this
subtype (sequences from 1A-1G, 3A-3G clusters, and lineage 2), by using different
modules with predefined parameters within the software packages CLC –
MainWorkbench and Mega X. The obtained ORF5 gene sequences were deposited in the
NCBI database. Phylogenetic analysis revealed that all PRRSV1 isolates detected in the
Serbia from 2012 to 2022 were grouped into two subtypes, subtype 1 and 3. Within
lineage 1, there were 7 isolates: 2 in cluster 1A, 2 in cluster 1B, 2 in cluster 1D, and one
isolate in cluster 1F. Within lineage 2, 29 sequences were grouped in cluster 3C.
The obtained results provide important information for future epidemiological studies of
PRRSV1 infection, including the relatedness between isolates, comparing new obtianed
sequences with sequences originating from vaccine strains, differentiating vaccine
strains from "wild" strains, and determining the introduction of new strains into the
population. Such epidemiological studies can be used for find critical points in the
biosecurity on the given farms and implement changes in the others to prevent and
control PRRSV infection.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
T1  - Genetski diverzitet ORF5 gena virusa reproduktivnog i respiratornog sindroma svinja tip 1 od 2012 do 2022 godine
T1  - Genetic diversity of the ORF5 gene of type 1 porcine reproductive and respiratory syndrome virus in Serbia from 2012 to 2022
EP  - 103
SP  - 102
ER  - 

@conference{
author = "Savić, Božidar and Milićević, Vesna and Kureljušić, Branislav and Jezdimirović, Nemanja and Milovanović, Bojan and Maksimović Zorić, Jelena and Stevnčević, Ognjen",
year = "2024",
abstract = "Репродуктивни и респираторни синдром свиња (енгл. Porcine Reproductive and Respiratory
Syndrome – PRRS), је контагиозна вирусна афекција свиња која тренутно представља
економски најзначајније инфективно обољење свиња, ендемично у многим земљама у
свету укључујучи и нашу земљу. PRRS је узрокован са вирусом репродуктивног и
респираторног синдрома свиња (енгл. Porcine Reproductive and Respiratory Syndrome Virus
– PRRSV), а с краја 90-их година прошлог века, када је обољење по први пут
дијагностиковано у нашој земљи, постоји релативно ограничен број епидемиолошких
студија овог обољења и молекуларних истраживања овог вируса који циркулишу у
популацији свиња. Описана су два генотипа PRRSV: тип-1 или Европски тип (Lelystad -
прототип) и тип-2 или Амерички тип (VR-2332 – прототип). Од недавно, PRRSV тип-1 и
тип-2 су класификовани као две засебне врсте унутар рода Betaarterivirus и то као
Betaarterivirus suid 1 (PRRSV1) и Betaarterivirus suid 2 (PRRSV2). У оквиру генотипа 1
(PRRSV1) се разликују 4 генетске линије (подтипа) (1, 2, 3 и 4) у које су сврстани изолати
вируса пореклом из различитих географских региона Европе. Унутар подтипова 1 и 3, се
диференцирају кластери 1А-1Г и 3А-3Г док подтип 2 чини јединствена група изолата, без
дефинисаних кластера. У нашој земљи, PRRS је био искључиво узрокован генотипом 1
(PRRSV1), међутим, 2022 године, детектован је и генотип 2 вируса (PRRSV2), на фарми
која је вршила увоз свиња из Европске уније. Анализом генома овог вируса је установљено
да је најсличнији тзв. вакциналним-сојевима (vaccine-like strain), односно да је вирус
највероватније пореклом из вакцине (МLV PRRSV2 вакцина) која је употребљена за
имунизацију свиња против PRRSV2 у земљи извозници. Циљ овог истраживањ је био да
се установи генетски диверзитет изолата PRRSV1 пореклом са фарми свиња из наше земље
у периоду од 2012 до 2022 године. У ту сврху, извршено је секвенцирање 36 ОРФ5 гена
изолата PRRSV1 коришћењем Сангер методе, које су потом поређене и анализиране
заједно са 106 секвенци ОРФ5 гена PRRSV1 из генетских линија 1, 2 и 3, које репрезентују
комплетан спектар генетског диверзитета ових подтипова (секвенце кластера 1А-1Г, 3А-
3Г и линије 2), применом различитих модула са предефинисаним параметарима у оквиру
софтверских пакета CLC – MainWorkbench и Mega X. Прикупљене секвнеце ОРФ5 гена су
депоноване у „NCBI“ бази биоинформатичких података. Филогенетском анализом је
установљено да су сви изолати PRRSV1 детектовани у периооду од 2012 до 2022 године у
нашој земљи груписани у два подтипа, односно подтип 1 и 3. У оквиру линије 1, се налази
7 изолата и то: 2 изолата у кластеру 1А, 2 у кластеру 1Б, 2 у кластеру 1Д и један изолат у
кластеру 1Ф. У оквиру линије 2, 29 секвенци је груписано у кластер 3Ц. Добијени
резултати представљају важне информације за будуће епидемиолошке анализе PRRSV1
инфекције укључујући утврђивање повезаности односно сродности између изолата вируса,
поређење нових секвенци са секвенцама пореклом од вакциналних сојева вируса,
разликовање вакциналних од „дивљих“ сојева вируса и утврђивање „уноса“ нових вируса
у популацију. Оваква епидемиолошка истраживања треба искористити за установљавање
критичних тачака у биосигурносним мерама на конкретним фармама, те имплементирати
одговарајуће промене како би се PRRSV инфекција успешно превенирала и контролисала., Porcine Reproductive and Respiratory Syndrome (PRRS) is a contagious viral disease
of swine that currently represents the most economically significant infectious disease
of pigs, endemic in many countries worldwide, including Serbia. PRRS is caused by the
Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). Since the late 1990s,
when the disease was first diagnosed in the Serbia, there have been only few
epidemiological and molecular studies on circulating PPRS-viruses. Two genotypes of
PRRSV have been described: type 1 or European type (prototype Lelystad) and type 2
or American type (prototype VR-2332). Recently, PRRSV type 1 and type 2 have been
classified as two separate species within the genus Betaarterivirus, namely
Betaarterivirus suid 1 (PRRSV1) and Betaarterivirus suid 2 (PRRSV2). Within
genotype 1 (PRRSV1), four genetic lineages (subtypes) (1, 2, 3, and 4) have been
distinguished, in which isolates from different geographic regions of Europe are
classified. Within subtypes 1 and 3, clusters 1A-1G and 3A-3G are further differentiated,
while subtype 2 consists of a unique group of isolates, without defined clusters. In Serbia,
PRRS was exclusively caused by genotype 1 (PRRSV1); however, in 2022, a genotype
2 (PRRSV2) was detected on a farm that imported pigs from the European Union.
Genome analysis of this virus revealed its similarity to vaccine-like strains, indicating
that the virus most likely originated from a vaccine (MLV PRRSV2 vaccine) used for
immunization against PRRSV2 in the exporting country.
The aim of this study was to establish the genetic diversity of PRRSV1 isolates
originating from pig farms in Serbia from 2012 to 2022. For this purpose, sequencing of
36 ORF5 gene of PRRSV1 was performed using the Sanger method, which were then
compared and analyzed together with 106 ORF5 gene sequences of PRRSV1 from
genetic lineages 1, 2, and 3, representing the full range of genetic diversity of this
subtype (sequences from 1A-1G, 3A-3G clusters, and lineage 2), by using different
modules with predefined parameters within the software packages CLC –
MainWorkbench and Mega X. The obtained ORF5 gene sequences were deposited in the
NCBI database. Phylogenetic analysis revealed that all PRRSV1 isolates detected in the
Serbia from 2012 to 2022 were grouped into two subtypes, subtype 1 and 3. Within
lineage 1, there were 7 isolates: 2 in cluster 1A, 2 in cluster 1B, 2 in cluster 1D, and one
isolate in cluster 1F. Within lineage 2, 29 sequences were grouped in cluster 3C.
The obtained results provide important information for future epidemiological studies of
PRRSV1 infection, including the relatedness between isolates, comparing new obtianed
sequences with sequences originating from vaccine strains, differentiating vaccine
strains from "wild" strains, and determining the introduction of new strains into the
population. Such epidemiological studies can be used for find critical points in the
biosecurity on the given farms and implement changes in the others to prevent and
control PRRSV infection.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.",
title = "Genetski diverzitet ORF5 gena virusa reproduktivnog i respiratornog sindroma svinja tip 1 od 2012 do 2022 godine, Genetic diversity of the ORF5 gene of type 1 porcine reproductive and respiratory syndrome virus in Serbia from 2012 to 2022",
pages = "103-102"
}

Savić, B., Milićević, V., Kureljušić, B., Jezdimirović, N., Milovanović, B., Maksimović Zorić, J.,& Stevnčević, O.. (2024). Genetski diverzitet ORF5 gena virusa reproduktivnog i respiratornog sindroma svinja tip 1 od 2012 do 2022 godine. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 102-103.

Savić B, Milićević V, Kureljušić B, Jezdimirović N, Milovanović B, Maksimović Zorić J, Stevnčević O. Genetski diverzitet ORF5 gena virusa reproduktivnog i respiratornog sindroma svinja tip 1 od 2012 do 2022 godine. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.. 2024;:102-103..

Savić, Božidar, Milićević, Vesna, Kureljušić, Branislav, Jezdimirović, Nemanja, Milovanović, Bojan, Maksimović Zorić, Jelena, Stevnčević, Ognjen, "Genetski diverzitet ORF5 gena virusa reproduktivnog i respiratornog sindroma svinja tip 1 od 2012 do 2022 godine" in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god. (2024):102-103.

TY  - CONF
AU  - Maksimović Zorić, Jelena
AU  - Veljović, Ljubiša
AU  - Glišić, Dimitrije
AU  - Kureljušić, Branislav
AU  - Jezdimirović, Nemanja
AU  - Stanojević, Slobodan
AU  - Maletić, Jelena
AU  - Ninković, Milan
AU  - Aksić, Đorđe
AU  - Savić, Božidar
PY  - 2024
UR  - https://reponivs.nivs.rs/handle/123456789/951
AB  - Афричка куга свиња (АКС) је први пут потврђена на територији Републике
Србије у јулу 2019. године, код домаћих свиња на епизоотиолошком подручју
Града Београда. Епизоотиолошким увиђајем је установљено да је неодговорно
понашање људи довело до уноса вируса из околних земаља и ширења заразе. У
првом таласу регистрована су жаришта у три насељена места, а применом
законом прописаних мера, болест је стављена под контролу и одјављена
децембра 2019. године. Упркос поновном уносу вируса из околних земаља и
ширењу заразе у популацијама дивљих и домаћих свиња у североисточним,
централним, источним и јужним деловима земље, ситуација на територији
епизоотиолошког подручја Града Београда у периоду од 2020. до 2022. године
је била стабилна. Поновно избијање заразе десило се 9. јуна 2023. године када
је болест потврђена код угинуле дивље свиње пронађене у ловишту
"Панчевачки рит", на територији општине Палилула. Већ 12. јуна потврђен је и
први случај код екстензивно узгајаних домаћих свиња у насљеном месту
Крњача. Зараза је убрзо потврђена и код дивљих свиња суседног ловишта "Рит"
и домаћих свиња других насељених места општине Палилула (Бесни Фок,
Дунавац, Јабучки Рит, Ковилово, Падинска Скела, Борча, Овча, Сланци, Велико
Село, Вишњица). У периоду од 9. јуна до 5. августа болест је потврђена на 157
газдинстава, односно код 223 домаће и код 110 дивљих свиња, те је посумњано
да су активности људи уз неадекватне биосигурносне мере на газдинствима
допринеле настанку епизоотије. Током јула и августа 2023. године зараза је
потврђена и код дивљих и домаћих свиња на територијама општина Земун,
Гроцка, Чукарица и Обреновац, где је регистровано 34 позитивна газдинстава и
једно ловиште. Од 26. септембра до 28. децембра није било нових случајева
заразе на епизоотиолошком подручју Града Београда, те је зараза одјављена 26.
децембра, односно 3 месеца после последњег потврђеног случаја АКС.
AB  - African swine fever (ASF) was first confirmed in the Republic of Serbia in July 2019,
in domestic pigs settled within the Belgrade epizootiology area. Through an
epizootiological investigation, it was concluded that the outbreak was a result of
irresponsible human behaviour which led to the introduction of the virus from
surrounding countries and its further spread. In the first wave, three outbreaks were
detected in three villages and by implementing regulatory measures for disease
control, the infection was successfully resolved in December 2019. Although the virus
re-entered from neighboring countries and spread across the northeast, central,
eastern, and southern regions, there were no new disease outbreaks in the Belgrade
epizootiological unit during the 2020-2022 period. Despite efforts to control the
infection, the disease reappeared in June 2023, spreading rapidly among wild boars
and domestic pigs in various settlements and hunting grounds of Palilula municipality.
During the two-month period, the disease was confirmed on 157 holdings,
respectively in 223 domestic pigs and 110 wild boars, implying the significance of
human behaviour and poor biosecurity measures on the epizootic spread of the
disease. During July and August 2023, the infection was confirmed in wild boars and
domestic pigs in the municipalities Zemun, Grocka, Čukarica, and Obrenovac, where
34 holdings and one hunting ground were declared positive. From September 26th to
December 28th, there were no new cases of infection in the Belgrade epizootiology
area, and the infection was resolved on December 26th, i.e. 3 months after the last
confirmed case of ASF.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
T1  - Afrička kuga svinja - epizootiološka situacija na teritoriji okruga grada Beograda u periodu od 2019. do 2023. godine
T1  - African swine fever - epizootiological situation in the Belgrade city area in the period from 2019 to 2023
EP  - 91
SP  - 90
ER  - 

@conference{
author = "Maksimović Zorić, Jelena and Veljović, Ljubiša and Glišić, Dimitrije and Kureljušić, Branislav and Jezdimirović, Nemanja and Stanojević, Slobodan and Maletić, Jelena and Ninković, Milan and Aksić, Đorđe and Savić, Božidar",
year = "2024",
abstract = "Афричка куга свиња (АКС) је први пут потврђена на територији Републике
Србије у јулу 2019. године, код домаћих свиња на епизоотиолошком подручју
Града Београда. Епизоотиолошким увиђајем је установљено да је неодговорно
понашање људи довело до уноса вируса из околних земаља и ширења заразе. У
првом таласу регистрована су жаришта у три насељена места, а применом
законом прописаних мера, болест је стављена под контролу и одјављена
децембра 2019. године. Упркос поновном уносу вируса из околних земаља и
ширењу заразе у популацијама дивљих и домаћих свиња у североисточним,
централним, источним и јужним деловима земље, ситуација на територији
епизоотиолошког подручја Града Београда у периоду од 2020. до 2022. године
је била стабилна. Поновно избијање заразе десило се 9. јуна 2023. године када
је болест потврђена код угинуле дивље свиње пронађене у ловишту
"Панчевачки рит", на територији општине Палилула. Већ 12. јуна потврђен је и
први случај код екстензивно узгајаних домаћих свиња у насљеном месту
Крњача. Зараза је убрзо потврђена и код дивљих свиња суседног ловишта "Рит"
и домаћих свиња других насељених места општине Палилула (Бесни Фок,
Дунавац, Јабучки Рит, Ковилово, Падинска Скела, Борча, Овча, Сланци, Велико
Село, Вишњица). У периоду од 9. јуна до 5. августа болест је потврђена на 157
газдинстава, односно код 223 домаће и код 110 дивљих свиња, те је посумњано
да су активности људи уз неадекватне биосигурносне мере на газдинствима
допринеле настанку епизоотије. Током јула и августа 2023. године зараза је
потврђена и код дивљих и домаћих свиња на територијама општина Земун,
Гроцка, Чукарица и Обреновац, где је регистровано 34 позитивна газдинстава и
једно ловиште. Од 26. септембра до 28. децембра није било нових случајева
заразе на епизоотиолошком подручју Града Београда, те је зараза одјављена 26.
децембра, односно 3 месеца после последњег потврђеног случаја АКС., African swine fever (ASF) was first confirmed in the Republic of Serbia in July 2019,
in domestic pigs settled within the Belgrade epizootiology area. Through an
epizootiological investigation, it was concluded that the outbreak was a result of
irresponsible human behaviour which led to the introduction of the virus from
surrounding countries and its further spread. In the first wave, three outbreaks were
detected in three villages and by implementing regulatory measures for disease
control, the infection was successfully resolved in December 2019. Although the virus
re-entered from neighboring countries and spread across the northeast, central,
eastern, and southern regions, there were no new disease outbreaks in the Belgrade
epizootiological unit during the 2020-2022 period. Despite efforts to control the
infection, the disease reappeared in June 2023, spreading rapidly among wild boars
and domestic pigs in various settlements and hunting grounds of Palilula municipality.
During the two-month period, the disease was confirmed on 157 holdings,
respectively in 223 domestic pigs and 110 wild boars, implying the significance of
human behaviour and poor biosecurity measures on the epizootic spread of the
disease. During July and August 2023, the infection was confirmed in wild boars and
domestic pigs in the municipalities Zemun, Grocka, Čukarica, and Obrenovac, where
34 holdings and one hunting ground were declared positive. From September 26th to
December 28th, there were no new cases of infection in the Belgrade epizootiology
area, and the infection was resolved on December 26th, i.e. 3 months after the last
confirmed case of ASF.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.",
title = "Afrička kuga svinja - epizootiološka situacija na teritoriji okruga grada Beograda u periodu od 2019. do 2023. godine, African swine fever - epizootiological situation in the Belgrade city area in the period from 2019 to 2023",
pages = "91-90"
}

Maksimović Zorić, J., Veljović, L., Glišić, D., Kureljušić, B., Jezdimirović, N., Stanojević, S., Maletić, J., Ninković, M., Aksić, Đ.,& Savić, B.. (2024). Afrička kuga svinja - epizootiološka situacija na teritoriji okruga grada Beograda u periodu od 2019. do 2023. godine. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 90-91.

Maksimović Zorić J, Veljović L, Glišić D, Kureljušić B, Jezdimirović N, Stanojević S, Maletić J, Ninković M, Aksić Đ, Savić B. Afrička kuga svinja - epizootiološka situacija na teritoriji okruga grada Beograda u periodu od 2019. do 2023. godine. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.. 2024;:90-91..

Maksimović Zorić, Jelena, Veljović, Ljubiša, Glišić, Dimitrije, Kureljušić, Branislav, Jezdimirović, Nemanja, Stanojević, Slobodan, Maletić, Jelena, Ninković, Milan, Aksić, Đorđe, Savić, Božidar, "Afrička kuga svinja - epizootiološka situacija na teritoriji okruga grada Beograda u periodu od 2019. do 2023. godine" in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god. (2024):90-91.

TY  - CONF
AU  - Kureljušić, Branislav
AU  - Vićić, Ivan
AU  - Bradić, Milijana
AU  - Maletić, Jelena
AU  - Jezdimirović, Nemanja
AU  - Savić, Božidar
AU  - Milovanović, Bojan
AU  - Karabasil, Neđeljko
PY  - 2024
UR  - https://reponivs.nivs.rs/handle/123456789/955
AB  - Интензивирањем бројлерске производње дошло је до значајног повећања
преваленције различитих патолошких стања чија основна етиологија почива у
самом технолошком процесу производње. Појава контактног дерматитиса на
плантарној кожи екстремитета („foot pad dermatitis“), затим на скочном зглобу
(„hock burns“) и на грудима („breast burns“) данас представља врло раширену
појаву у интензивној производњи, наносећи значајне економске губитке услед
смањеног прираста и одбацивања трупова на линији клања. Поред интензивирања
бројлерске производње значајан утицај на њихову појаву имају исхрана, услови и
начин држања (врста простирке) и генетска предиспозиција. Најзначајнији
фактор ризика за настанак лезија је влажна простирка посебно у комбинацији са
штетним дејством амонијака. Појава лезија има веома велики значај као
индикатор добробити имајући у виду да постоје претпоставке да су установљене
лезије врло болне и да често онемогућавају нормално кретање пилића.
Макроскопским прегледом лезија на плантарној површини уочавају се
инфламаторне лезије праћене некрозом на јастучићима табана и/или на прстима.
Лезије се ређе могу установити на тибиометатарзалним зглобовима. Код благог
облика постоји дисколорација на кожи, хиперкератоза, а у тежим случајевима
јавња се едем, ерозије и улцерације.
Хистопатолошки, благе лезије се карактеришу умереном хиперплазијом и/или
хиперкератозом епидермиса, конгестијом и едемом, уз присуство благог
инфламаторног инфилтрата у форми периваскулитиса и субепителијалне
инфилтрације мононуклеарним ћелијама и мањим бројем гранулоцита. Умерене
суперфицијелне лезије се карактеришу врло израженом хиперплазијом и
хиперкератозом епидермиса, егзоцитозом, појавом пустула и красти, конгестијом
и инфилтрацијом дермиса мононуклеарним и гранулоцитиним инфилтратом.
Тешке, дубоке лезије се карактеришу некрозом епидермиса који је замењен
некротичним гнојним садржајем, појавом гранулационог ткива, израженом
инфламаторном инфилтрацијом (периваскулитис и субепителна инфилтрација)
доминантно хетерофилним гранулоцитима и мононуклеарним ћелијама.
Имајући у виду да је појава контактног дерматитиса све учесталија појава како на
фармама бројлерских пилића тако и на кланици приликом прегледа трупова на
линија клања неопходно је имплементирати програм контроле овог патолошког
стања и на тај начин побољшати добробит и обезбедити производњу квалитетног
и безбедног пилећег меса.
AB  - The intensification of broiler production has led to a significant increase in the
prevalence of various pathological conditions, whose basic etiology lies in the
production process itself. The occurrence of contact dermatitis on the plantar skin of
extremities ("foot pad dermatitis"), then on the hock joint ("hock burns"), and on the
breasts ("breast burns") is today a very widespread phenomenon in intensive production,
causing significant economic losses due to reduced growth and carcass rejection on the
slaughter line. In addition to the intensification of broiler production, significant factors
influencing their occurrence are nutrition, housing conditions (type of bedding), and
genetic predisposition. The most significant risk factor for lesion formation is wet
bedding, especially in combination with the harmful effects of ammonia. The
occurrence of lesions is very important as an indicator of welfare, considering that there
are assumptions that established lesions are very painful and often prevent normal
chicken movement. Macroscopic examination of lesions on the plantar surface reveals
inflammatory lesions accompanied by necrosis on the foot pads and/or toes. Lesions are
less commonly found on the tibiotarsal joints. In mild forms, discoloration of the skin,
hyperkeratosis, and in severe cases, edema, erosions, and ulcerations occur.
Histopathologically, mild lesions are characterized by moderate epidermal hyperplasia
and/or hyperkeratosis, congestion and edema, with the presence of mild inflammatory
infiltrate in the form of perivasculitis and subepithelial infiltration of mononuclear cells
and a small number of granulocytes. Moderate superficial lesions are characterized by
very pronounced epidermal hyperplasia and hyperkeratosis, exocytosis, appearance of
pustules and crusts, congestion, and infiltration of the dermis with mononuclear and
granulocytic infiltrate. Severe, deep lesions are characterized by epidermal necrosis
replaced by necrotic purulent content, appearance of granulation tissue, pronounced
inflammatory infiltration (perivasculitis and subepithelial infiltration) predominantly
with heterophilic granulocytes and mononuclear cells. Considering that the occurrence
of contact dermatitis is becoming more frequent both on broiler farms and in
slaughterhouses during carcass examination on the slaughter line, it is necessary to
implement a control program for this pathological condition
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
T1  - Kontaktni pododermatitis kod brojlerskih pilića – patološki aspekt -
T1  - Contact pododermatitis in broiler chickens – pathological aspect –
EP  - 147
SP  - 146
ER  - 

@conference{
author = "Kureljušić, Branislav and Vićić, Ivan and Bradić, Milijana and Maletić, Jelena and Jezdimirović, Nemanja and Savić, Božidar and Milovanović, Bojan and Karabasil, Neđeljko",
year = "2024",
abstract = "Интензивирањем бројлерске производње дошло је до значајног повећања
преваленције различитих патолошких стања чија основна етиологија почива у
самом технолошком процесу производње. Појава контактног дерматитиса на
плантарној кожи екстремитета („foot pad dermatitis“), затим на скочном зглобу
(„hock burns“) и на грудима („breast burns“) данас представља врло раширену
појаву у интензивној производњи, наносећи значајне економске губитке услед
смањеног прираста и одбацивања трупова на линији клања. Поред интензивирања
бројлерске производње значајан утицај на њихову појаву имају исхрана, услови и
начин држања (врста простирке) и генетска предиспозиција. Најзначајнији
фактор ризика за настанак лезија је влажна простирка посебно у комбинацији са
штетним дејством амонијака. Појава лезија има веома велики значај као
индикатор добробити имајући у виду да постоје претпоставке да су установљене
лезије врло болне и да често онемогућавају нормално кретање пилића.
Макроскопским прегледом лезија на плантарној површини уочавају се
инфламаторне лезије праћене некрозом на јастучићима табана и/или на прстима.
Лезије се ређе могу установити на тибиометатарзалним зглобовима. Код благог
облика постоји дисколорација на кожи, хиперкератоза, а у тежим случајевима
јавња се едем, ерозије и улцерације.
Хистопатолошки, благе лезије се карактеришу умереном хиперплазијом и/или
хиперкератозом епидермиса, конгестијом и едемом, уз присуство благог
инфламаторног инфилтрата у форми периваскулитиса и субепителијалне
инфилтрације мононуклеарним ћелијама и мањим бројем гранулоцита. Умерене
суперфицијелне лезије се карактеришу врло израженом хиперплазијом и
хиперкератозом епидермиса, егзоцитозом, појавом пустула и красти, конгестијом
и инфилтрацијом дермиса мононуклеарним и гранулоцитиним инфилтратом.
Тешке, дубоке лезије се карактеришу некрозом епидермиса који је замењен
некротичним гнојним садржајем, појавом гранулационог ткива, израженом
инфламаторном инфилтрацијом (периваскулитис и субепителна инфилтрација)
доминантно хетерофилним гранулоцитима и мононуклеарним ћелијама.
Имајући у виду да је појава контактног дерматитиса све учесталија појава како на
фармама бројлерских пилића тако и на кланици приликом прегледа трупова на
линија клања неопходно је имплементирати програм контроле овог патолошког
стања и на тај начин побољшати добробит и обезбедити производњу квалитетног
и безбедног пилећег меса., The intensification of broiler production has led to a significant increase in the
prevalence of various pathological conditions, whose basic etiology lies in the
production process itself. The occurrence of contact dermatitis on the plantar skin of
extremities ("foot pad dermatitis"), then on the hock joint ("hock burns"), and on the
breasts ("breast burns") is today a very widespread phenomenon in intensive production,
causing significant economic losses due to reduced growth and carcass rejection on the
slaughter line. In addition to the intensification of broiler production, significant factors
influencing their occurrence are nutrition, housing conditions (type of bedding), and
genetic predisposition. The most significant risk factor for lesion formation is wet
bedding, especially in combination with the harmful effects of ammonia. The
occurrence of lesions is very important as an indicator of welfare, considering that there
are assumptions that established lesions are very painful and often prevent normal
chicken movement. Macroscopic examination of lesions on the plantar surface reveals
inflammatory lesions accompanied by necrosis on the foot pads and/or toes. Lesions are
less commonly found on the tibiotarsal joints. In mild forms, discoloration of the skin,
hyperkeratosis, and in severe cases, edema, erosions, and ulcerations occur.
Histopathologically, mild lesions are characterized by moderate epidermal hyperplasia
and/or hyperkeratosis, congestion and edema, with the presence of mild inflammatory
infiltrate in the form of perivasculitis and subepithelial infiltration of mononuclear cells
and a small number of granulocytes. Moderate superficial lesions are characterized by
very pronounced epidermal hyperplasia and hyperkeratosis, exocytosis, appearance of
pustules and crusts, congestion, and infiltration of the dermis with mononuclear and
granulocytic infiltrate. Severe, deep lesions are characterized by epidermal necrosis
replaced by necrotic purulent content, appearance of granulation tissue, pronounced
inflammatory infiltration (perivasculitis and subepithelial infiltration) predominantly
with heterophilic granulocytes and mononuclear cells. Considering that the occurrence
of contact dermatitis is becoming more frequent both on broiler farms and in
slaughterhouses during carcass examination on the slaughter line, it is necessary to
implement a control program for this pathological condition",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.",
title = "Kontaktni pododermatitis kod brojlerskih pilića – patološki aspekt -, Contact pododermatitis in broiler chickens – pathological aspect –",
pages = "147-146"
}

Kureljušić, B., Vićić, I., Bradić, M., Maletić, J., Jezdimirović, N., Savić, B., Milovanović, B.,& Karabasil, N.. (2024). Kontaktni pododermatitis kod brojlerskih pilića – patološki aspekt -. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 146-147.

Kureljušić B, Vićić I, Bradić M, Maletić J, Jezdimirović N, Savić B, Milovanović B, Karabasil N. Kontaktni pododermatitis kod brojlerskih pilića – patološki aspekt -. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.. 2024;:146-147..

Kureljušić, Branislav, Vićić, Ivan, Bradić, Milijana, Maletić, Jelena, Jezdimirović, Nemanja, Savić, Božidar, Milovanović, Bojan, Karabasil, Neđeljko, "Kontaktni pododermatitis kod brojlerskih pilića – patološki aspekt -" in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god. (2024):146-147.

TY  - CONF
AU  - Vasić, Ana
AU  - Kureljušić, Jasna
AU  - Rokvić, Nikola
AU  - Glišić, Dimitrije
AU  - Milovanović, Bojan
AU  - Vasilev, Saša
AU  - Kureljušić, Branislav
AU  - Paunović, Milan
PY  - 2024
UR  - https://reponivs.nivs.rs/handle/123456789/949
AB  - Трихинелоза је широм света присутна зооноза која се преноси конзумирањем
сировог или неправилно термички обрађеног меса или производа од меса
дивљих и домаћих животиња заражених ларвама нематода рода Trichinella.
Дивљи месоједи имају значајну епидемиолошку улогу у силватичном циклусу у
Европи, најчешће ширење Trichinella spp. међу животињама је путем исхране
кроз конзумацију меса одбаченог приликом лова и ингестије лешева који у
мишићном ткиву садрже ларве трихинела.
Од четири врсте Trichinella присутне у Европи (T. spiralis, T. britovi, T. nativa и
T. pseudospiralis), у Србији је присуство Т. spiralis и Т. britovi потврђено код
дивљих месоједа. Иако су статус и трендови популација дивљих карнивора у
Србији стабилни, енормна експанзија популације шакала (Canis aureus) током
последњих 40 година, донела је нове изазове у контроли Trichinella spp.
Циљ нашег истраживања је био да стекнемо увид у преваленцију Trichinella spp.
у популацији дивљих месоједа ( шакали (Canis aureus), лисице (Vulpas vulpes),
дивље мачке (Felis silvaticus) и јазавци (Meles meles)) који су изловљени на три
локалитета који припадају Срему, Граду Београду и Браничевском региону и да
идентификујемо врсте дивљих. месоједа које учествују у природном циклусу
преношења Trichinella spp. у Србији.
Укупно 122 узорка мускулатуре језика животиња одстрељених током 2022. и
2023. године испитано је појединачно коришћењем метода вештачке дигестије.
Тестирано је 67 узорака пореклом од шакала, 43 узорка од лисице, 10 узорака од
јазаваца и 2 од дивљих мачака. Резултати су показали да је 26 (21,14 %) било
позитивно на присуство ларви Trichinella spp. Међу позитивним узорцима је
највише било пореклом од шакала 18 (69,23 %), 7 (26,92 %) узорака пореклом од
лисице, 1 (3,85 %) дивљих мачака и 0 позитивних јазаваца.
Шакали, лисице и дивље мачке укључени су у циклус преноса Trichinella spp. на
основу наших резултата. Молекуларно одређивање врсте трихинеле је
неопходно како би се боље разумели начини преноса и како спречити ширење
дивљих на домаће животиње.
AB  - Trichinellosis is a worldwide zoonosis acquired by consuming raw or improperly
cooked meat or meat-derived products from wild and domestic animals infected with
larvae of nematode worms of the genus Trichinella. Wild carnivores have major
epidemiological role in the sylvatic cycle in Europe, mostly spreading Trichinella spp.
within animals through carnivorous activities and by their scavenger behaviour.
From four Trichinella species present in Europe (namely T. spiralis, T. britovi, T.
nativa and T. pseudospiralis), in Serbia the presence of T. spiralis and T. britovi is
confirmed in wild carnivores. Although the status and trends of the populations of
wild carnivores in Serbia are stable, enormous expansion of Golden jackal (Canis
aureus) population during last 40 years has brought new challenge in the control of
Trichinella spp.
The aim of our research was to access the prevalence of Trichinella spp. in population
of wild carnivores (Golden jackal (Canis aureus), fox (Vulpes vulpes), wild cat (Felis
silvestris) and badger (Meles meles) in three districts in Serbia (Braničevski, Sremski
and City of Belgrade) and to identify wild carnivore species taking part of the natural
transmission cycle of Trichinella spp. in Serbia.
A total of 122 tongue muscle tissue samples of animals hunted down during 2022 and
2023 were examined using artificial digestion method. We tested 67 samples from
jackal, 43 from foxes, 10 from badgers i 2 from wild cats. The results showed that 26
(21.14 %) were positive for the presence of Trichinella spp. larvae, among them 18
(69.23 %) jackals, 7 (26.92 %) foxes, one (3.85 %) wild cat, while none was positive
from badgers.
Jackals, foxes and wild cats are involved in Trichinella spp. transmission cycle.
Further Trichinella species typing is necessary to better understand modes of
transmission and how to prevent spreading from wild to domestic animals.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
T1  - Trihineloza kod divljih mesojeda i njihov značaj u održanju Trichinella spp.
T1  - Trichinelosis in wild carnivores and their significance in natural cycle of Trichinella spp.
EP  - 35
SP  - 34
ER  - 

@conference{
author = "Vasić, Ana and Kureljušić, Jasna and Rokvić, Nikola and Glišić, Dimitrije and Milovanović, Bojan and Vasilev, Saša and Kureljušić, Branislav and Paunović, Milan",
year = "2024",
abstract = "Трихинелоза је широм света присутна зооноза која се преноси конзумирањем
сировог или неправилно термички обрађеног меса или производа од меса
дивљих и домаћих животиња заражених ларвама нематода рода Trichinella.
Дивљи месоједи имају значајну епидемиолошку улогу у силватичном циклусу у
Европи, најчешће ширење Trichinella spp. међу животињама је путем исхране
кроз конзумацију меса одбаченог приликом лова и ингестије лешева који у
мишићном ткиву садрже ларве трихинела.
Од четири врсте Trichinella присутне у Европи (T. spiralis, T. britovi, T. nativa и
T. pseudospiralis), у Србији је присуство Т. spiralis и Т. britovi потврђено код
дивљих месоједа. Иако су статус и трендови популација дивљих карнивора у
Србији стабилни, енормна експанзија популације шакала (Canis aureus) током
последњих 40 година, донела је нове изазове у контроли Trichinella spp.
Циљ нашег истраживања је био да стекнемо увид у преваленцију Trichinella spp.
у популацији дивљих месоједа ( шакали (Canis aureus), лисице (Vulpas vulpes),
дивље мачке (Felis silvaticus) и јазавци (Meles meles)) који су изловљени на три
локалитета који припадају Срему, Граду Београду и Браничевском региону и да
идентификујемо врсте дивљих. месоједа које учествују у природном циклусу
преношења Trichinella spp. у Србији.
Укупно 122 узорка мускулатуре језика животиња одстрељених током 2022. и
2023. године испитано је појединачно коришћењем метода вештачке дигестије.
Тестирано је 67 узорака пореклом од шакала, 43 узорка од лисице, 10 узорака од
јазаваца и 2 од дивљих мачака. Резултати су показали да је 26 (21,14 %) било
позитивно на присуство ларви Trichinella spp. Међу позитивним узорцима је
највише било пореклом од шакала 18 (69,23 %), 7 (26,92 %) узорака пореклом од
лисице, 1 (3,85 %) дивљих мачака и 0 позитивних јазаваца.
Шакали, лисице и дивље мачке укључени су у циклус преноса Trichinella spp. на
основу наших резултата. Молекуларно одређивање врсте трихинеле је
неопходно како би се боље разумели начини преноса и како спречити ширење
дивљих на домаће животиње., Trichinellosis is a worldwide zoonosis acquired by consuming raw or improperly
cooked meat or meat-derived products from wild and domestic animals infected with
larvae of nematode worms of the genus Trichinella. Wild carnivores have major
epidemiological role in the sylvatic cycle in Europe, mostly spreading Trichinella spp.
within animals through carnivorous activities and by their scavenger behaviour.
From four Trichinella species present in Europe (namely T. spiralis, T. britovi, T.
nativa and T. pseudospiralis), in Serbia the presence of T. spiralis and T. britovi is
confirmed in wild carnivores. Although the status and trends of the populations of
wild carnivores in Serbia are stable, enormous expansion of Golden jackal (Canis
aureus) population during last 40 years has brought new challenge in the control of
Trichinella spp.
The aim of our research was to access the prevalence of Trichinella spp. in population
of wild carnivores (Golden jackal (Canis aureus), fox (Vulpes vulpes), wild cat (Felis
silvestris) and badger (Meles meles) in three districts in Serbia (Braničevski, Sremski
and City of Belgrade) and to identify wild carnivore species taking part of the natural
transmission cycle of Trichinella spp. in Serbia.
A total of 122 tongue muscle tissue samples of animals hunted down during 2022 and
2023 were examined using artificial digestion method. We tested 67 samples from
jackal, 43 from foxes, 10 from badgers i 2 from wild cats. The results showed that 26
(21.14 %) were positive for the presence of Trichinella spp. larvae, among them 18
(69.23 %) jackals, 7 (26.92 %) foxes, one (3.85 %) wild cat, while none was positive
from badgers.
Jackals, foxes and wild cats are involved in Trichinella spp. transmission cycle.
Further Trichinella species typing is necessary to better understand modes of
transmission and how to prevent spreading from wild to domestic animals.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.",
title = "Trihineloza kod divljih mesojeda i njihov značaj u održanju Trichinella spp., Trichinelosis in wild carnivores and their significance in natural cycle of Trichinella spp.",
pages = "35-34"
}

Vasić, A., Kureljušić, J., Rokvić, N., Glišić, D., Milovanović, B., Vasilev, S., Kureljušić, B.,& Paunović, M.. (2024). Trihineloza kod divljih mesojeda i njihov značaj u održanju Trichinella spp.. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 34-35.

Vasić A, Kureljušić J, Rokvić N, Glišić D, Milovanović B, Vasilev S, Kureljušić B, Paunović M. Trihineloza kod divljih mesojeda i njihov značaj u održanju Trichinella spp.. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.. 2024;:34-35..

Vasić, Ana, Kureljušić, Jasna, Rokvić, Nikola, Glišić, Dimitrije, Milovanović, Bojan, Vasilev, Saša, Kureljušić, Branislav, Paunović, Milan, "Trihineloza kod divljih mesojeda i njihov značaj u održanju Trichinella spp." in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god. (2024):34-35.

TY  - CONF
AU  - Glišić, Dimitrije
AU  - Zurovac Sapundžić, Zorana
AU  - Šolaja, Sofija
AU  - Kureljušić, Branislav
AU  - Vasković, Nikola
AU  - Đorđević, Milutin
AU  - Mirčeta, Jovan
AU  - Milićević, Vesna
PY  - 2024
UR  - https://reponivs.nivs.rs/handle/123456789/950
AB  - Значај дивљих животиња у ширењу и одржавању заразних болести у
ветеринарској медицини је често занемарен, посебно њихов утицај на
отежавање искорењивања болести код домаћих животиња, истичући потребу за
темељним истраживањем. У овој студији смо хтели да одредимо
серопреваленцију и просторну дистрибуцију одабраних заразних болести међу
популацијама дивљих преживара и то: обичног јелена, јелена лопатара, срне и
муфлона у Србији. Анализирано је 259 узорака серума дивљих преживара.
Узорци су тестирани на присуство антитела на низ патогена, укључујући вирус
плавог језика, Шмаленберг вирус, вирус говеђе дијареје/бордерске болести,
Capripox вирус, вирус Западног Нила, херпес вирус 1 говеда, Кју грознице,
Brucella spp. и Leptospira spp. Присуство антитела на Capripox вирус и
Leptospira spp. није детектовано, што сугерише њихово одсуство у проучаваној
популацији. Код једног узорка детектовано је присуство антитела на вирус
говеђе дијареје/бордерске болести са 98,8% поузданости, што имплицира да је
преваленција ове болести врло ниска у популацији и да је могућност за
ереадикацију сасвим извесна. Надаље, антитела против Brucella spp. и Кју
грознице детектована су код неколико животиња, тачније код једне, односно
три јединке, док су антитела на херпес вирус 1 говеда пронађена у 20,85%
узорака. Серопреваленција за векторске болести је износила 20,5% за
Шмаленберг вирус, 34,3% за грозницу Западног Нила и 38,6% за болест плавог
језика. Ови резултати наглашавају сложене факторе које утичу на здравље
дивљих животиња, укључујући преваленцију болести код домаћих животиња,
карактеристике патогена, услове околине и утицај вектора. Стога је неопходно
размотрити дивље животиње не само као фактор ризика већ као кључан извор
информација за разумевање преваленције болести и механизме њиховог
ширења.
AB  - The often-overlooked importance of wildlife in spreading and maintaining animals'
infectious diseases, especially their impact on complicating the eradication of diseases
in domestic animals, highlights the need for thorough research. In this study, we
wanted to ascertain the seroprevalence and spatial distribution of selected infectious
diseases among wild ruminant populations in Serbia. An analysis was performed
testing 259 wild ruminant sera samples, for the presence of antibodies to certain
pathogens, including bluetongue virus, Schmallenberg virus, Bovine viral
diarrhoea/border disease virus, Capripox virus, West Nile fever virus, Bovine herpes
virus-1, Coxiella burnetii, Brucella spp., and Leptospira spp. Antibodies against
Capripox virus and Leptospira spp., were not detected, suggesting their absence in the
studied population. Antibodies against Bovine viral diarrhoea/border disease virus
were detected in only one sample with 98.8% confidence, implying a minimal
presence of Bovine viral diarrhoea and potentially indicating a status of near
eradication within the examined population. Furthermore, antibodies against Brucella
spp. and Coxiella burnetii were detected only in a few animals, one and three animals,
respectively, while Bovine herpes virus-1 antibodies were found in 20.85% of the
samples. Seroprevalence for vector-borne diseases was recorded at 20.5% for
Schmallenberg disease, 34.3% for West Nile fever, and 38.6% for Bluetongue. These
results elucidate the complex interdependencies influencing the health of wildlife,
which include the prevalence of diseases in domestic animals, pathogen traits,
environmental conditions, and vector capabilities. Therefore, it is imperative to
consider wildlife not solely as a vector of risk, but as a pivotal source of information
for the understanding of disease prevalence and the mechanisms of its spread.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
T1  - Seroprevalencija specifičnih zaraznih bolesti kod divljih preživara u Srbiji
T1  - Seroprevalence analysis of specific infectious diseases in wild ruminants across Serbia
EP  - 77
SP  - 76
ER  - 

@conference{
author = "Glišić, Dimitrije and Zurovac Sapundžić, Zorana and Šolaja, Sofija and Kureljušić, Branislav and Vasković, Nikola and Đorđević, Milutin and Mirčeta, Jovan and Milićević, Vesna",
year = "2024",
abstract = "Значај дивљих животиња у ширењу и одржавању заразних болести у
ветеринарској медицини је често занемарен, посебно њихов утицај на
отежавање искорењивања болести код домаћих животиња, истичући потребу за
темељним истраживањем. У овој студији смо хтели да одредимо
серопреваленцију и просторну дистрибуцију одабраних заразних болести међу
популацијама дивљих преживара и то: обичног јелена, јелена лопатара, срне и
муфлона у Србији. Анализирано је 259 узорака серума дивљих преживара.
Узорци су тестирани на присуство антитела на низ патогена, укључујући вирус
плавог језика, Шмаленберг вирус, вирус говеђе дијареје/бордерске болести,
Capripox вирус, вирус Западног Нила, херпес вирус 1 говеда, Кју грознице,
Brucella spp. и Leptospira spp. Присуство антитела на Capripox вирус и
Leptospira spp. није детектовано, што сугерише њихово одсуство у проучаваној
популацији. Код једног узорка детектовано је присуство антитела на вирус
говеђе дијареје/бордерске болести са 98,8% поузданости, што имплицира да је
преваленција ове болести врло ниска у популацији и да је могућност за
ереадикацију сасвим извесна. Надаље, антитела против Brucella spp. и Кју
грознице детектована су код неколико животиња, тачније код једне, односно
три јединке, док су антитела на херпес вирус 1 говеда пронађена у 20,85%
узорака. Серопреваленција за векторске болести је износила 20,5% за
Шмаленберг вирус, 34,3% за грозницу Западног Нила и 38,6% за болест плавог
језика. Ови резултати наглашавају сложене факторе које утичу на здравље
дивљих животиња, укључујући преваленцију болести код домаћих животиња,
карактеристике патогена, услове околине и утицај вектора. Стога је неопходно
размотрити дивље животиње не само као фактор ризика већ као кључан извор
информација за разумевање преваленције болести и механизме њиховог
ширења., The often-overlooked importance of wildlife in spreading and maintaining animals'
infectious diseases, especially their impact on complicating the eradication of diseases
in domestic animals, highlights the need for thorough research. In this study, we
wanted to ascertain the seroprevalence and spatial distribution of selected infectious
diseases among wild ruminant populations in Serbia. An analysis was performed
testing 259 wild ruminant sera samples, for the presence of antibodies to certain
pathogens, including bluetongue virus, Schmallenberg virus, Bovine viral
diarrhoea/border disease virus, Capripox virus, West Nile fever virus, Bovine herpes
virus-1, Coxiella burnetii, Brucella spp., and Leptospira spp. Antibodies against
Capripox virus and Leptospira spp., were not detected, suggesting their absence in the
studied population. Antibodies against Bovine viral diarrhoea/border disease virus
were detected in only one sample with 98.8% confidence, implying a minimal
presence of Bovine viral diarrhoea and potentially indicating a status of near
eradication within the examined population. Furthermore, antibodies against Brucella
spp. and Coxiella burnetii were detected only in a few animals, one and three animals,
respectively, while Bovine herpes virus-1 antibodies were found in 20.85% of the
samples. Seroprevalence for vector-borne diseases was recorded at 20.5% for
Schmallenberg disease, 34.3% for West Nile fever, and 38.6% for Bluetongue. These
results elucidate the complex interdependencies influencing the health of wildlife,
which include the prevalence of diseases in domestic animals, pathogen traits,
environmental conditions, and vector capabilities. Therefore, it is imperative to
consider wildlife not solely as a vector of risk, but as a pivotal source of information
for the understanding of disease prevalence and the mechanisms of its spread.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.",
title = "Seroprevalencija specifičnih zaraznih bolesti kod divljih preživara u Srbiji, Seroprevalence analysis of specific infectious diseases in wild ruminants across Serbia",
pages = "77-76"
}

Glišić, D., Zurovac Sapundžić, Z., Šolaja, S., Kureljušić, B., Vasković, N., Đorđević, M., Mirčeta, J.,& Milićević, V.. (2024). Seroprevalencija specifičnih zaraznih bolesti kod divljih preživara u Srbiji. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 76-77.

Glišić D, Zurovac Sapundžić Z, Šolaja S, Kureljušić B, Vasković N, Đorđević M, Mirčeta J, Milićević V. Seroprevalencija specifičnih zaraznih bolesti kod divljih preživara u Srbiji. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.. 2024;:76-77..

Glišić, Dimitrije, Zurovac Sapundžić, Zorana, Šolaja, Sofija, Kureljušić, Branislav, Vasković, Nikola, Đorđević, Milutin, Mirčeta, Jovan, Milićević, Vesna, "Seroprevalencija specifičnih zaraznih bolesti kod divljih preživara u Srbiji" in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god. (2024):76-77.

TY  - CONF
AU  - Šolaja, Sofija
AU  - Glišić, Dimitrije
AU  - Zurovac Sapundžić, Zorana
AU  - Milovanović, Bojan
AU  - Maletić, Jelena
AU  - Jezdimirović, Nemanja
AU  - Kureljušić, Branislav
AU  - Milićević, Vesna
PY  - 2024
UR  - https://reponivs.nivs.rs/handle/123456789/954
AB  - Инфективне болести представљају један од главних проблема у интензивном узгоју свиња, упркос технолошком напретку и развоју биосигурносних мера. Вирусна респираторна обољења која имају највећи економски и здравствени значај у свињарству су репродуктивни и респираторни синдром свиња, инфлуенца свиња и Аујецкијева болест. Сеоска газдинства екстензивног типа, и даље предcтављају најчешћи начин узгоја свиња у Србији. Циљ истраживања је био да се утврди серопреваленција респираторних вируса на сеоским газдинствима која представљају извор патогена за комерцијалне фарме. Крвни серуми су узорковани од 222 јединке са 69 газдинстава на којима се налазило до 10 свиња. За детекцију антитела су коришћени комерцијални ЕЛИСА тестови према упутству произвођача. Установљено је 38 (17,1%) серопозитивних јединки на вирус Аујецкијеве болести и 6 (2,7%) на вирус репродуктивног и респираторног синдрома. Антитела на вирус инфлуенце свиња нису установљена ни код једне јединкe. Два газдинства (2,9%) су била серопозитивна на репродуктивни и респираторни синдром са серопреваленцијом у запату од 100%, без присуства антитела на друге тестиране вирусе. Проценат серопозитивних запата на Аујецкијеву болест је био 10,1%, а серопреваленција у запатима је варирала од 33% до 100%. Серонегативних запата на све три болести је било 48 (69,5%). Ови резултати указују да међу свињама на сеоским газдинствима највише циркулише вирус Аујецкијеве болести. Главне катактеристике сеоских газдинстава су неадекватне биосигурносне мере, исхрана помијама и неконтролисан транспорт и контакт са другим врстама животиња. Стога, будући да је здравствени статус оваквих запата непознат, они се сматрају извором високог ризика за комерцијалне фарме. Власници сеоских газдинстава су често запослени на комерцијалним фармама, без обзира на забрану држања свиња, и представљају везу између сеоских газдинстава и комерцијалних фарми. Неопходно је спровести мониторниг здравственог стања, побољшање биосигурносних мера и едукацију власника како би се спречило ширење инфективних обољења са сеоских газдинстава на комерцијалне фарме.
AB  - Infectious diseases represent one of the main challenges in intensive pig farming, despite technological advancements and biosecurity improvement. Viral respiratory diseases with the greatest economic and health significance in pig farming include porcine reproductive and respiratory syndrome (PRRS), swine influenza (SI), and Aujeszky's disease (AD). Small backyard farms continue to be the most common method of pig farming in Serbia. This research aimed to assess the prevalence of respiratory viruses on backyard farms, which serve as a source of pathogens for commercial farms. Serum samples were collected from 222 individuals on 69 farms, each housing up to 10 pigs. Commercial ELISA tests were used for antibody detection according to the manufacturer's instructions. Out of the samples, 38 (17.1%) were seropositive for AD, and 6 (2.7%) for PRRS. No antibodies to the swine influenza virus were detected. Two farms (2.9%) were seropositive for PRRS with a herd-level seroprevalence of 100%, with no antibodies detected for other tested viruses. The percentage of seropositive farms for Aujeszky's disease was 10.1%, with herd-level seroprevalence ranging from 33% to 100%. There were 48 (69.5%) seronegative herds for all three diseases. These results indicate that Aujeszky's disease virus circulates the most among pigs on backyard farms. Main characteristics of backyard farms are low biosecurity, swill feeding, uncontrolled trade and contact with other animal species. Thus, since the health status of backyard farms is unknown, they are considered a high-risk infection source for commercial farms. Owners of backyard farms are often employed on commercial farms, despite regulations, serving as a link between backyard and commercial farms. Implementation of monitoring, improvement of biosecurity measures and education of owners are crucial steps for preventing the transmission of infectious diseases between backyard and commercial farms.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
T1  - Seroprevalencija virusnih respiratornih oboljenja svinja na seoskim gazdinstvima u Srbiji
T1  - Seroprevalence of viral respiratory diseases in swine backyard farms in Serbia
EP  - 107
SP  - 106
ER  - 

@conference{
author = "Šolaja, Sofija and Glišić, Dimitrije and Zurovac Sapundžić, Zorana and Milovanović, Bojan and Maletić, Jelena and Jezdimirović, Nemanja and Kureljušić, Branislav and Milićević, Vesna",
year = "2024",
abstract = "Инфективне болести представљају један од главних проблема у интензивном узгоју свиња, упркос технолошком напретку и развоју биосигурносних мера. Вирусна респираторна обољења која имају највећи економски и здравствени значај у свињарству су репродуктивни и респираторни синдром свиња, инфлуенца свиња и Аујецкијева болест. Сеоска газдинства екстензивног типа, и даље предcтављају најчешћи начин узгоја свиња у Србији. Циљ истраживања је био да се утврди серопреваленција респираторних вируса на сеоским газдинствима која представљају извор патогена за комерцијалне фарме. Крвни серуми су узорковани од 222 јединке са 69 газдинстава на којима се налазило до 10 свиња. За детекцију антитела су коришћени комерцијални ЕЛИСА тестови према упутству произвођача. Установљено је 38 (17,1%) серопозитивних јединки на вирус Аујецкијеве болести и 6 (2,7%) на вирус репродуктивног и респираторног синдрома. Антитела на вирус инфлуенце свиња нису установљена ни код једне јединкe. Два газдинства (2,9%) су била серопозитивна на репродуктивни и респираторни синдром са серопреваленцијом у запату од 100%, без присуства антитела на друге тестиране вирусе. Проценат серопозитивних запата на Аујецкијеву болест је био 10,1%, а серопреваленција у запатима је варирала од 33% до 100%. Серонегативних запата на све три болести је било 48 (69,5%). Ови резултати указују да међу свињама на сеоским газдинствима највише циркулише вирус Аујецкијеве болести. Главне катактеристике сеоских газдинстава су неадекватне биосигурносне мере, исхрана помијама и неконтролисан транспорт и контакт са другим врстама животиња. Стога, будући да је здравствени статус оваквих запата непознат, они се сматрају извором високог ризика за комерцијалне фарме. Власници сеоских газдинстава су често запослени на комерцијалним фармама, без обзира на забрану држања свиња, и представљају везу између сеоских газдинстава и комерцијалних фарми. Неопходно је спровести мониторниг здравственог стања, побољшање биосигурносних мера и едукацију власника како би се спречило ширење инфективних обољења са сеоских газдинстава на комерцијалне фарме., Infectious diseases represent one of the main challenges in intensive pig farming, despite technological advancements and biosecurity improvement. Viral respiratory diseases with the greatest economic and health significance in pig farming include porcine reproductive and respiratory syndrome (PRRS), swine influenza (SI), and Aujeszky's disease (AD). Small backyard farms continue to be the most common method of pig farming in Serbia. This research aimed to assess the prevalence of respiratory viruses on backyard farms, which serve as a source of pathogens for commercial farms. Serum samples were collected from 222 individuals on 69 farms, each housing up to 10 pigs. Commercial ELISA tests were used for antibody detection according to the manufacturer's instructions. Out of the samples, 38 (17.1%) were seropositive for AD, and 6 (2.7%) for PRRS. No antibodies to the swine influenza virus were detected. Two farms (2.9%) were seropositive for PRRS with a herd-level seroprevalence of 100%, with no antibodies detected for other tested viruses. The percentage of seropositive farms for Aujeszky's disease was 10.1%, with herd-level seroprevalence ranging from 33% to 100%. There were 48 (69.5%) seronegative herds for all three diseases. These results indicate that Aujeszky's disease virus circulates the most among pigs on backyard farms. Main characteristics of backyard farms are low biosecurity, swill feeding, uncontrolled trade and contact with other animal species. Thus, since the health status of backyard farms is unknown, they are considered a high-risk infection source for commercial farms. Owners of backyard farms are often employed on commercial farms, despite regulations, serving as a link between backyard and commercial farms. Implementation of monitoring, improvement of biosecurity measures and education of owners are crucial steps for preventing the transmission of infectious diseases between backyard and commercial farms.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.",
title = "Seroprevalencija virusnih respiratornih oboljenja svinja na seoskim gazdinstvima u Srbiji, Seroprevalence of viral respiratory diseases in swine backyard farms in Serbia",
pages = "107-106"
}

Šolaja, S., Glišić, D., Zurovac Sapundžić, Z., Milovanović, B., Maletić, J., Jezdimirović, N., Kureljušić, B.,& Milićević, V.. (2024). Seroprevalencija virusnih respiratornih oboljenja svinja na seoskim gazdinstvima u Srbiji. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 106-107.

Šolaja S, Glišić D, Zurovac Sapundžić Z, Milovanović B, Maletić J, Jezdimirović N, Kureljušić B, Milićević V. Seroprevalencija virusnih respiratornih oboljenja svinja na seoskim gazdinstvima u Srbiji. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.. 2024;:106-107..

Šolaja, Sofija, Glišić, Dimitrije, Zurovac Sapundžić, Zorana, Milovanović, Bojan, Maletić, Jelena, Jezdimirović, Nemanja, Kureljušić, Branislav, Milićević, Vesna, "Seroprevalencija virusnih respiratornih oboljenja svinja na seoskim gazdinstvima u Srbiji" in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god. (2024):106-107.

TY  - JOUR
AU  - Milićević, Vesna
AU  - Zurovac Sapundžić, Zorana
AU  - Glišić, Dimitrije
AU  - Kureljušić, Branislav
AU  - Vasković, Nikola
AU  - Đorđević, Milutin
AU  - Mirčeta, Jovan
PY  - 2024
UR  - https://reponivs.nivs.rs/handle/123456789/907
AB  - The role of wildlife in maintaining infectious diseases in veterinary medicine is often neglected, although the
disease eradication process in domestic animals is continuously affected by the risk of pathogens transmission
from wildlife as a primary source. The main aim of this paper was to estimate the prevalence and distribution of
selected infectious diseases in wild ruminants in Serbia. In total, 259 sera from wild ruminants were tested for
specific antibodies to bluetongue virus, Schmallenberg virus, Bovine viral diarrhea/border disease virus, Capripox
virus, West Nile fever virus, Bovine herpes virus-1, Coxiella burnetii, Brucella spp., and Leptospira spp.
Specific Capripox virus and Leptospira spp. antibodies were not detected in any of the 259 wild ruminant
samples. Although one animal was detected positive for BVDV/BDV specific antibodies, with 99.8% confidence,
the prevalence of BVD within this population could be very low i.e. essentially free from BVD infection. One and
three positive animals were detected for Brucella spp. and Coxiella burnetii antibodies, respectively. Bovine herpes
virus-1 specific antibodies were detected in 20.85% of the samples. The estimated seroprevalence of vector-borne
diseases was 20.5% for Schmallenberg disease, 34.3% for West Nile fever, and 38.6% for Bluetongue.
Considering the reported results, wildlife health status is a result of different factors in complex relation, such
as the presence of disease in domestic animals, disease nature, pathogen characteristics, environmental factors,
presence, and vector competence. Wildlife should be considered not only as a risk but as a source of important
information on disease distribution and its indicators.
PB  - Elsevier
T2  - Research in Veterinary Science
T1  - Cross-sectional serosurvey of selected infectious diseases in wild ruminants in Serbia
SP  - 105183
VL  - 170
DO  - 10.1016/j.rvsc.2024.105183
ER  - 

@article{
author = "Milićević, Vesna and Zurovac Sapundžić, Zorana and Glišić, Dimitrije and Kureljušić, Branislav and Vasković, Nikola and Đorđević, Milutin and Mirčeta, Jovan",
year = "2024",
abstract = "The role of wildlife in maintaining infectious diseases in veterinary medicine is often neglected, although the
disease eradication process in domestic animals is continuously affected by the risk of pathogens transmission
from wildlife as a primary source. The main aim of this paper was to estimate the prevalence and distribution of
selected infectious diseases in wild ruminants in Serbia. In total, 259 sera from wild ruminants were tested for
specific antibodies to bluetongue virus, Schmallenberg virus, Bovine viral diarrhea/border disease virus, Capripox
virus, West Nile fever virus, Bovine herpes virus-1, Coxiella burnetii, Brucella spp., and Leptospira spp.
Specific Capripox virus and Leptospira spp. antibodies were not detected in any of the 259 wild ruminant
samples. Although one animal was detected positive for BVDV/BDV specific antibodies, with 99.8% confidence,
the prevalence of BVD within this population could be very low i.e. essentially free from BVD infection. One and
three positive animals were detected for Brucella spp. and Coxiella burnetii antibodies, respectively. Bovine herpes
virus-1 specific antibodies were detected in 20.85% of the samples. The estimated seroprevalence of vector-borne
diseases was 20.5% for Schmallenberg disease, 34.3% for West Nile fever, and 38.6% for Bluetongue.
Considering the reported results, wildlife health status is a result of different factors in complex relation, such
as the presence of disease in domestic animals, disease nature, pathogen characteristics, environmental factors,
presence, and vector competence. Wildlife should be considered not only as a risk but as a source of important
information on disease distribution and its indicators.",
publisher = "Elsevier",
journal = "Research in Veterinary Science",
title = "Cross-sectional serosurvey of selected infectious diseases in wild ruminants in Serbia",
pages = "105183",
volume = "170",
doi = "10.1016/j.rvsc.2024.105183"
}

Milićević, V., Zurovac Sapundžić, Z., Glišić, D., Kureljušić, B., Vasković, N., Đorđević, M.,& Mirčeta, J.. (2024). Cross-sectional serosurvey of selected infectious diseases in wild ruminants in Serbia. in Research in Veterinary Science
Elsevier., 170, 105183.
https://doi.org/10.1016/j.rvsc.2024.105183

Milićević V, Zurovac Sapundžić Z, Glišić D, Kureljušić B, Vasković N, Đorđević M, Mirčeta J. Cross-sectional serosurvey of selected infectious diseases in wild ruminants in Serbia. in Research in Veterinary Science. 2024;170:105183.
doi:10.1016/j.rvsc.2024.105183 .

Milićević, Vesna, Zurovac Sapundžić, Zorana, Glišić, Dimitrije, Kureljušić, Branislav, Vasković, Nikola, Đorđević, Milutin, Mirčeta, Jovan, "Cross-sectional serosurvey of selected infectious diseases in wild ruminants in Serbia" in Research in Veterinary Science, 170 (2024):105183,
https://doi.org/10.1016/j.rvsc.2024.105183 . .

TY  - JOUR
AU  - Bojkovski, Jovan
AU  - Prodanov-Radulović, Jasna
AU  - Nedić, Sreten
AU  - Arsić, Sveta
AU  - Đurić, Miloje
AU  - Mitrović, Aleksandra
AU  - Žutić, Jadranka
AU  - Kureljušić, Jasna
AU  - Pavlović, Ivan
AU  - Zdravković, Nemanja
AU  - Kureljušić, Branislav
AU  - Angjelovski, Branko
AU  - Anita, Dragoş Constantin
AU  - Oslbanu, Luanda
AU  - Anita, Adriana Elena
AU  - Prodanović, Radiša
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/861
AB  - Mycopasmatic  or enzootic pneumonia is the most common disease of the respiratory system under in the intensive pig production. It is clinically manifested by coughing, a chronic inflammatory process in the lungs, high morbidity and a relatively low percentage of deaths. The infection can be  transmitted horizontally and vertically. Mycoplasma hyopneumoniae invades the epithelial cells of the trachea, bronchi, bronchioles and alveoli and disrupts the function of the ciliary body. Evagination of epithelial cells occurs, so that the cleansing of the airway mucosa by the mucociliary apparatus is inhibited. As a result, bacterial complications (Pasteurella, Bordetella, Klebsiella, Actinobacillus, Hemophilus) are common.M.hyopneminia can play imortant role in PRDC. The development of  Mycoplasma hyopneumoniae is favoured by large congregations of pigs in small spaces, inadequate environmental conditions (microclimate), parasitic infections and inadequate nutrition. Mycoplasma hyopneumonia  can be a significant health problem on the fattening farm, exacerbated by the influence of non-specific factors as well as the spread of other bacterial pathogens.
PB  - Iasi : Ion Ionescu de la Brad
T2  - Scientific papers - Veterinary Medicine
T1  - Mycoplasmatic (enzootic) pneumonia of pigs as a health problem in fattening units
EP  - 43
IS  - 3
SP  - 35
VL  - 66
ER  - 

@article{
author = "Bojkovski, Jovan and Prodanov-Radulović, Jasna and Nedić, Sreten and Arsić, Sveta and Đurić, Miloje and Mitrović, Aleksandra and Žutić, Jadranka and Kureljušić, Jasna and Pavlović, Ivan and Zdravković, Nemanja and Kureljušić, Branislav and Angjelovski, Branko and Anita, Dragoş Constantin and Oslbanu, Luanda and Anita, Adriana Elena and Prodanović, Radiša",
year = "2023",
abstract = "Mycopasmatic  or enzootic pneumonia is the most common disease of the respiratory system under in the intensive pig production. It is clinically manifested by coughing, a chronic inflammatory process in the lungs, high morbidity and a relatively low percentage of deaths. The infection can be  transmitted horizontally and vertically. Mycoplasma hyopneumoniae invades the epithelial cells of the trachea, bronchi, bronchioles and alveoli and disrupts the function of the ciliary body. Evagination of epithelial cells occurs, so that the cleansing of the airway mucosa by the mucociliary apparatus is inhibited. As a result, bacterial complications (Pasteurella, Bordetella, Klebsiella, Actinobacillus, Hemophilus) are common.M.hyopneminia can play imortant role in PRDC. The development of  Mycoplasma hyopneumoniae is favoured by large congregations of pigs in small spaces, inadequate environmental conditions (microclimate), parasitic infections and inadequate nutrition. Mycoplasma hyopneumonia  can be a significant health problem on the fattening farm, exacerbated by the influence of non-specific factors as well as the spread of other bacterial pathogens.",
publisher = "Iasi : Ion Ionescu de la Brad",
journal = "Scientific papers - Veterinary Medicine",
title = "Mycoplasmatic (enzootic) pneumonia of pigs as a health problem in fattening units",
pages = "43-35",
number = "3",
volume = "66"
}

Bojkovski, J., Prodanov-Radulović, J., Nedić, S., Arsić, S., Đurić, M., Mitrović, A., Žutić, J., Kureljušić, J., Pavlović, I., Zdravković, N., Kureljušić, B., Angjelovski, B., Anita, D. C., Oslbanu, L., Anita, A. E.,& Prodanović, R.. (2023). Mycoplasmatic (enzootic) pneumonia of pigs as a health problem in fattening units. in Scientific papers - Veterinary Medicine
Iasi : Ion Ionescu de la Brad., 66(3), 35-43.

Bojkovski J, Prodanov-Radulović J, Nedić S, Arsić S, Đurić M, Mitrović A, Žutić J, Kureljušić J, Pavlović I, Zdravković N, Kureljušić B, Angjelovski B, Anita DC, Oslbanu L, Anita AE, Prodanović R. Mycoplasmatic (enzootic) pneumonia of pigs as a health problem in fattening units. in Scientific papers - Veterinary Medicine. 2023;66(3):35-43..

Bojkovski, Jovan, Prodanov-Radulović, Jasna, Nedić, Sreten, Arsić, Sveta, Đurić, Miloje, Mitrović, Aleksandra, Žutić, Jadranka, Kureljušić, Jasna, Pavlović, Ivan, Zdravković, Nemanja, Kureljušić, Branislav, Angjelovski, Branko, Anita, Dragoş Constantin, Oslbanu, Luanda, Anita, Adriana Elena, Prodanović, Radiša, "Mycoplasmatic (enzootic) pneumonia of pigs as a health problem in fattening units" in Scientific papers - Veterinary Medicine, 66, no. 3 (2023):35-43.

TY  - CONF
AU  - Maletić, Jelena
AU  - Pajić, Marko
AU  - Đurđević, Biljana
AU  - Kureljušić, Branislav
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/685
AB  - The World Organisation for Animal Health (WOAH, founded as OIE) is an international, intergovernmental organization responsible for standard-setting processes on animal health and welfare since its establishment in 1924. Terrestrial and Aquatic Animal Health Codes of WOAH provide internationally recognized science-based standards for improving animal health and welfare and veterinary public health worldwide, including through means for safe international trade in terrestrial and aquatic animals and their products. The Manuals of Diagnostic Tests and Vaccines for Terrestrial and Aquatic Animals provide a standardized approach to diagnosing the diseases listed in the Terrestrial and Aquatic Codes.
For the WOAH and its 182 Members, biosecurity is defined as “a set of management and physical measures designed to reduce the risk of introduction, establishment and spread of animal diseases, infections or infestations to, from and within an animal population”. Although there is no specific chapter on biosecurity in WOAH international standard yet, the term biosecurity is mentioned 264 times across 12 sections and 45 chapters of Terrestrial Code, which recognizes the importance of biosecurity in the whole veterinary domain: for prevention and control of animal diseases, improving animal health and protecting public health, for animal welfare purposes, safe trade of live animals and veterinary commodities, etc.
However, WOAH recognizes biosecurity as essential for disease prevention and control, thereby improving animal health and welfare. Therefore, an ad hoc group of recognized international experts has been designated by the Director General of WOAH to start drafting a specific chapter on biosecurity. This work will follow WOAH’s standards-setting process which includes consultation with WOAH Members before being proposed for adoption by the World Assembly of Delegates at the WOAH General Assembly. The role of the scientific community and national research entities is key for developing further these standards and their implementation by Members.
PB  - Ghent : Faculty of veterinary medicine
C3  - COST action CA20103 "Biosecurity Enhanced Through Training Evaluation and Raising Awareness"
T1  - Biosecurity measures on the broilers and breeders' farms in Serbia
EP  - 31
SP  - 31
ER  - 

@conference{
author = "Maletić, Jelena and Pajić, Marko and Đurđević, Biljana and Kureljušić, Branislav",
year = "2023",
abstract = "The World Organisation for Animal Health (WOAH, founded as OIE) is an international, intergovernmental organization responsible for standard-setting processes on animal health and welfare since its establishment in 1924. Terrestrial and Aquatic Animal Health Codes of WOAH provide internationally recognized science-based standards for improving animal health and welfare and veterinary public health worldwide, including through means for safe international trade in terrestrial and aquatic animals and their products. The Manuals of Diagnostic Tests and Vaccines for Terrestrial and Aquatic Animals provide a standardized approach to diagnosing the diseases listed in the Terrestrial and Aquatic Codes.
For the WOAH and its 182 Members, biosecurity is defined as “a set of management and physical measures designed to reduce the risk of introduction, establishment and spread of animal diseases, infections or infestations to, from and within an animal population”. Although there is no specific chapter on biosecurity in WOAH international standard yet, the term biosecurity is mentioned 264 times across 12 sections and 45 chapters of Terrestrial Code, which recognizes the importance of biosecurity in the whole veterinary domain: for prevention and control of animal diseases, improving animal health and protecting public health, for animal welfare purposes, safe trade of live animals and veterinary commodities, etc.
However, WOAH recognizes biosecurity as essential for disease prevention and control, thereby improving animal health and welfare. Therefore, an ad hoc group of recognized international experts has been designated by the Director General of WOAH to start drafting a specific chapter on biosecurity. This work will follow WOAH’s standards-setting process which includes consultation with WOAH Members before being proposed for adoption by the World Assembly of Delegates at the WOAH General Assembly. The role of the scientific community and national research entities is key for developing further these standards and their implementation by Members.",
publisher = "Ghent : Faculty of veterinary medicine",
journal = "COST action CA20103 "Biosecurity Enhanced Through Training Evaluation and Raising Awareness"",
title = "Biosecurity measures on the broilers and breeders' farms in Serbia",
pages = "31-31"
}

Maletić, J., Pajić, M., Đurđević, B.,& Kureljušić, B.. (2023). Biosecurity measures on the broilers and breeders' farms in Serbia. in COST action CA20103 "Biosecurity Enhanced Through Training Evaluation and Raising Awareness"
Ghent : Faculty of veterinary medicine., 31-31.

Maletić J, Pajić M, Đurđević B, Kureljušić B. Biosecurity measures on the broilers and breeders' farms in Serbia. in COST action CA20103 "Biosecurity Enhanced Through Training Evaluation and Raising Awareness". 2023;:31-31..

Maletić, Jelena, Pajić, Marko, Đurđević, Biljana, Kureljušić, Branislav, "Biosecurity measures on the broilers and breeders' farms in Serbia" in COST action CA20103 "Biosecurity Enhanced Through Training Evaluation and Raising Awareness" (2023):31-31.

TY  - CONF
AU  - Milovanović, Bojan
AU  - Stanojević, Slobodan
AU  - Kureljušić, Branislav
AU  - Zurovac Sapundžić, Zorana
AU  - Milićević, Vesna
AU  - Zdravković, Nemanja
AU  - Jezdimirović, Nemanja
AU  - Maletić, Milan
AU  - Savić, Božidar
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/816
AB  - Pojava povremenih pobačaja ne predstavlja razlog za zabrinutost. Dva glavna
kriterijuma kada pojava pobačaja postaje problem u zapatu su: ako stopa
pobačaja pređe 3% na 100 steonih krava ili 5% na 100 jagnjenih ovaca, ili
ukoliko veći broj životinja pobaci u kratkom vremenskom intervalu. U bilo koja
dva navedena slučaja neophodno je obavestiti ordinirajućeg veterinara i nadležnog
veterinarskog inspektora. Pobačaji se mogu podeliti na infektivne i neinfektivne.
Pobačaje infektivne prirode uzrokuju različiti virusi, bakterije (Brucella
abortus, Brucella ovis, Chlamydia abortus), gljivice, protozoe (Neospora
caninum) i rikecije (Coxiella burnetii). Uzročnici neinfektivne prirode mogu
biti: otrovne biljke, metabolički i hormonalni poremećaji, nutritivni deficiti,
traume i kongenitalne anomalije ploda. Etiološka dijagnoza pobačaja postavlja
se u svega 30% slučajeva – kod 40 do 50% slučajeva nije moguće utvrditi
uzrok. Podaci o zastupljenosti infektivnih agenasa kao uzročnika pobačaja variraju
u zavisnosti od regiona, načina držanja i uzoraka koji se dostavljaju na
laboratorijsko ispitivanje. Pored direktnog uticaja na zdravlje i dobrobit životinja,
pobačaji dovode i do ekonomskih gubitaka. Pojedini infektivni uzročnici
imaju zoonotski potencijal i predstavljaju opasnost po javno zdravlje. Zbog toga
prevencija pobačaja predstavlja imperativ u procesu proizvodnje.
AB  - The occurrence of periodical abortions is not a reason for concern. Two main
criteria when the abortions represent a problem on farm are: abortion rate
exceeds 3% per 100 pregnant cows or 5% per 100 pregnant ewes; certain
number of animals abort in a short time interval (abortion storm). In many of
two mentioned cases, it is necessary to inform the ordinating veterinarian and
the regional veterinary inspector. Etiology of abortions can be divided into
infectious and noninfectious. Infectious causes of abortions are viruses, bacteria
(Brucella abortus, Brucella ovis, Chlamydia abortus), fungi, protozoan (Neospora
caninum), rickettsias (Coxiella burnetii). Noninfectious abortions can be
caused by: heat stress, poisonous plants, metabolic and hormonal disorders,
nutritional deficits, trauma and congenital anomalies of fetus. The etiological
diagnosis of abortions is determinated in 30% of cases. In 40 to 50% of cases,
depending on the available laboratory diagnostics, it is not possible to establish
the cause. Data about prevalence of infectious agents vary depending on the
region, management and the samples that were submitted to diagnostic laboratory.
A spite of direct influence on animal health and welfare, abortions lead
to economic losses. Some infectious agents have zoonotic potential and represent
a risk to public health. Therefore, abortion control represent imperative in
the production process.
PB  - Beograd : Fakultet veterinarske medicine
C3  - Četvrti regionalni simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 29. jun - 1. jul 2023
T1  - Infektivni pobačaji preživara – zdravstveni i ekonomski značaj
T1  - Infectious abortions in ruminants–health and economic impact
EP  - 215
SP  - 207
ER  - 

@conference{
author = "Milovanović, Bojan and Stanojević, Slobodan and Kureljušić, Branislav and Zurovac Sapundžić, Zorana and Milićević, Vesna and Zdravković, Nemanja and Jezdimirović, Nemanja and Maletić, Milan and Savić, Božidar",
year = "2023",
abstract = "Pojava povremenih pobačaja ne predstavlja razlog za zabrinutost. Dva glavna
kriterijuma kada pojava pobačaja postaje problem u zapatu su: ako stopa
pobačaja pređe 3% na 100 steonih krava ili 5% na 100 jagnjenih ovaca, ili
ukoliko veći broj životinja pobaci u kratkom vremenskom intervalu. U bilo koja
dva navedena slučaja neophodno je obavestiti ordinirajućeg veterinara i nadležnog
veterinarskog inspektora. Pobačaji se mogu podeliti na infektivne i neinfektivne.
Pobačaje infektivne prirode uzrokuju različiti virusi, bakterije (Brucella
abortus, Brucella ovis, Chlamydia abortus), gljivice, protozoe (Neospora
caninum) i rikecije (Coxiella burnetii). Uzročnici neinfektivne prirode mogu
biti: otrovne biljke, metabolički i hormonalni poremećaji, nutritivni deficiti,
traume i kongenitalne anomalije ploda. Etiološka dijagnoza pobačaja postavlja
se u svega 30% slučajeva – kod 40 do 50% slučajeva nije moguće utvrditi
uzrok. Podaci o zastupljenosti infektivnih agenasa kao uzročnika pobačaja variraju
u zavisnosti od regiona, načina držanja i uzoraka koji se dostavljaju na
laboratorijsko ispitivanje. Pored direktnog uticaja na zdravlje i dobrobit životinja,
pobačaji dovode i do ekonomskih gubitaka. Pojedini infektivni uzročnici
imaju zoonotski potencijal i predstavljaju opasnost po javno zdravlje. Zbog toga
prevencija pobačaja predstavlja imperativ u procesu proizvodnje., The occurrence of periodical abortions is not a reason for concern. Two main
criteria when the abortions represent a problem on farm are: abortion rate
exceeds 3% per 100 pregnant cows or 5% per 100 pregnant ewes; certain
number of animals abort in a short time interval (abortion storm). In many of
two mentioned cases, it is necessary to inform the ordinating veterinarian and
the regional veterinary inspector. Etiology of abortions can be divided into
infectious and noninfectious. Infectious causes of abortions are viruses, bacteria
(Brucella abortus, Brucella ovis, Chlamydia abortus), fungi, protozoan (Neospora
caninum), rickettsias (Coxiella burnetii). Noninfectious abortions can be
caused by: heat stress, poisonous plants, metabolic and hormonal disorders,
nutritional deficits, trauma and congenital anomalies of fetus. The etiological
diagnosis of abortions is determinated in 30% of cases. In 40 to 50% of cases,
depending on the available laboratory diagnostics, it is not possible to establish
the cause. Data about prevalence of infectious agents vary depending on the
region, management and the samples that were submitted to diagnostic laboratory.
A spite of direct influence on animal health and welfare, abortions lead
to economic losses. Some infectious agents have zoonotic potential and represent
a risk to public health. Therefore, abortion control represent imperative in
the production process.",
publisher = "Beograd : Fakultet veterinarske medicine",
journal = "Četvrti regionalni simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 29. jun - 1. jul 2023",
title = "Infektivni pobačaji preživara – zdravstveni i ekonomski značaj, Infectious abortions in ruminants–health and economic impact",
pages = "215-207"
}

Milovanović, B., Stanojević, S., Kureljušić, B., Zurovac Sapundžić, Z., Milićević, V., Zdravković, N., Jezdimirović, N., Maletić, M.,& Savić, B.. (2023). Infektivni pobačaji preživara – zdravstveni i ekonomski značaj. in Četvrti regionalni simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 29. jun - 1. jul 2023
Beograd : Fakultet veterinarske medicine., 207-215.

Milovanović B, Stanojević S, Kureljušić B, Zurovac Sapundžić Z, Milićević V, Zdravković N, Jezdimirović N, Maletić M, Savić B. Infektivni pobačaji preživara – zdravstveni i ekonomski značaj. in Četvrti regionalni simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 29. jun - 1. jul 2023. 2023;:207-215..

Milovanović, Bojan, Stanojević, Slobodan, Kureljušić, Branislav, Zurovac Sapundžić, Zorana, Milićević, Vesna, Zdravković, Nemanja, Jezdimirović, Nemanja, Maletić, Milan, Savić, Božidar, "Infektivni pobačaji preživara – zdravstveni i ekonomski značaj" in Četvrti regionalni simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 29. jun - 1. jul 2023 (2023):207-215.

TY  - JOUR
AU  - Maksimović Zorić, Jelena
AU  - Milićević, Vesna
AU  - Veljović, Ljubiša
AU  - Radosavljević, Vladimir
AU  - Kureljušić, Branislav
AU  - Stevančević, Ognjen
AU  - Chiapponi, Chiara
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/913
AB  - Swine  influenza  presents  a  very  important  health  and  economic  issue  in  pig  productions  worldwide.  Viruses  that  cause  the  disease  are  genetically  very  diverse  but  usually  belong  to  the  H1N1,  H1N2  and  H3N2 subtype of influenza A viruses. In this study, we sequenced and analyzed the full genome of viruses detected  in  swine  from  seven  commercial  farms.  Through  the  analysis  of  the  complete  sequences  of  internal gene cassette together with previously characterized HA and NA genes we found three different genotypes  amongst  five  completely  sequenced  viruses.  Two  viruses  possessed  a  completely  H1avN1  genotype (40%) and belonged to the H1avN1 lineage, which is prevalent in European swine populations. The other three viruses have arisen through the reassortment of the genes of H1avN1 and H1N1pdm09 lineages. In one sample we detected coinfection with viruses of H3N2 subtype with genes of H1avN1, H1N1pdm09 and A/swine/Gent/1/1984-like H3N2 lineages that presents a potential environment for the generation of a triple reassortant virus. The presence of the H1N1pdm09 origin M gene in this sample implies the potential risk of the introduction of these viruses into the human population. Phylogenetic analysis of internal gene cassette revealed slower evolution within genes of H1N1pdm09 lineage than those of H1avN1 lineage.
PB  - Teramo : Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘G. Caporale’
T2  - Veterinaria Italiana
T1  - Genetic analysis of influenza A viruses in pigs from commercial farms in Serbia
IS  - 2
VL  - 59
DO  - 10.12834/VetIt.2712.17810.2
ER  - 

@article{
author = "Maksimović Zorić, Jelena and Milićević, Vesna and Veljović, Ljubiša and Radosavljević, Vladimir and Kureljušić, Branislav and Stevančević, Ognjen and Chiapponi, Chiara",
year = "2023",
abstract = "Swine  influenza  presents  a  very  important  health  and  economic  issue  in  pig  productions  worldwide.  Viruses  that  cause  the  disease  are  genetically  very  diverse  but  usually  belong  to  the  H1N1,  H1N2  and  H3N2 subtype of influenza A viruses. In this study, we sequenced and analyzed the full genome of viruses detected  in  swine  from  seven  commercial  farms.  Through  the  analysis  of  the  complete  sequences  of  internal gene cassette together with previously characterized HA and NA genes we found three different genotypes  amongst  five  completely  sequenced  viruses.  Two  viruses  possessed  a  completely  H1avN1  genotype (40%) and belonged to the H1avN1 lineage, which is prevalent in European swine populations. The other three viruses have arisen through the reassortment of the genes of H1avN1 and H1N1pdm09 lineages. In one sample we detected coinfection with viruses of H3N2 subtype with genes of H1avN1, H1N1pdm09 and A/swine/Gent/1/1984-like H3N2 lineages that presents a potential environment for the generation of a triple reassortant virus. The presence of the H1N1pdm09 origin M gene in this sample implies the potential risk of the introduction of these viruses into the human population. Phylogenetic analysis of internal gene cassette revealed slower evolution within genes of H1N1pdm09 lineage than those of H1avN1 lineage.",
publisher = "Teramo : Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘G. Caporale’",
journal = "Veterinaria Italiana",
title = "Genetic analysis of influenza A viruses in pigs from commercial farms in Serbia",
number = "2",
volume = "59",
doi = "10.12834/VetIt.2712.17810.2"
}

Maksimović Zorić, J., Milićević, V., Veljović, L., Radosavljević, V., Kureljušić, B., Stevančević, O.,& Chiapponi, C.. (2023). Genetic analysis of influenza A viruses in pigs from commercial farms in Serbia. in Veterinaria Italiana
Teramo : Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘G. Caporale’., 59(2).
https://doi.org/10.12834/VetIt.2712.17810.2

Maksimović Zorić J, Milićević V, Veljović L, Radosavljević V, Kureljušić B, Stevančević O, Chiapponi C. Genetic analysis of influenza A viruses in pigs from commercial farms in Serbia. in Veterinaria Italiana. 2023;59(2).
doi:10.12834/VetIt.2712.17810.2 .

Maksimović Zorić, Jelena, Milićević, Vesna, Veljović, Ljubiša, Radosavljević, Vladimir, Kureljušić, Branislav, Stevančević, Ognjen, Chiapponi, Chiara, "Genetic analysis of influenza A viruses in pigs from commercial farms in Serbia" in Veterinaria Italiana, 59, no. 2 (2023),
https://doi.org/10.12834/VetIt.2712.17810.2 . .

TY  - JOUR
AU  - Glišić, Dimitrije
AU  - Veljović, Ljubiša
AU  - Milovanović, Bojan
AU  - Ninković, Milan
AU  - Maletić, Jelena
AU  - Kureljušić, Branislav
AU  - Milićević, Vesna
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/849
AB  - Monitoring the health of swine herds is essential to ensure good manufacturing
practices. Traditionally, active and passive surveillance on farms involved invasive
sampling methods, where specific animals were selected, restrained, and sampled.
However, with the increasing intensity of swine production, alternative methods for
effective herd surveillance became necessary.
Non-invasive sampling provides a convenient and cost-effective approach to monitor
the entire herd without compromising animal welfare, while still obtaining suitable
samples for testing. Oral fluids have been widely used in both human and livestock
health surveillance for various viral pathogens, including significant diseases. Nasal
wipes (NW) utilize different cloth materials soaked in phosphate-buffered saline
(PBS) or tissue culture medium with antibiotics and antimycotics to sample for swine
influenza virus (SIV). Udder skin wipes (USW) offer an alternative method to assess
the health status of piglets in a litter. During routine procedures such as tail docking
and castration, a mixture of blood and serum can be collected, known as process fluids
(PF), which has proven successful in monitoring herds for the presence of porcine
reproductive and respiratory syndrome virus (PRRSV). Furthermore, air sampling has emerged as a novel technique to detect pathogens in various farming systems and
animal species. This method offers the advantage of obtaining diagnostic samples
without direct animal contact.
By employing these non-invasive sampling methods, swine producers can implement
effective surveillance strategies while maintaining animal welfare standards and
obtaining reliable diagnostic information.
PB  - Beograd : Fakultet veterinarske medicine, Univerzitet u Beogradu
PB  - Beograd : Veterinarska komora Srbije
T2  - Veterinarski glasnik
T1  - Overview of non-invasive sampling methods used in intensive swine farming
EP  - 108
IS  - 2
SP  - 97
VL  - 77
DO  - 10.2298/VETGL230614004G
ER  - 

@article{
author = "Glišić, Dimitrije and Veljović, Ljubiša and Milovanović, Bojan and Ninković, Milan and Maletić, Jelena and Kureljušić, Branislav and Milićević, Vesna",
year = "2023",
abstract = "Monitoring the health of swine herds is essential to ensure good manufacturing
practices. Traditionally, active and passive surveillance on farms involved invasive
sampling methods, where specific animals were selected, restrained, and sampled.
However, with the increasing intensity of swine production, alternative methods for
effective herd surveillance became necessary.
Non-invasive sampling provides a convenient and cost-effective approach to monitor
the entire herd without compromising animal welfare, while still obtaining suitable
samples for testing. Oral fluids have been widely used in both human and livestock
health surveillance for various viral pathogens, including significant diseases. Nasal
wipes (NW) utilize different cloth materials soaked in phosphate-buffered saline
(PBS) or tissue culture medium with antibiotics and antimycotics to sample for swine
influenza virus (SIV). Udder skin wipes (USW) offer an alternative method to assess
the health status of piglets in a litter. During routine procedures such as tail docking
and castration, a mixture of blood and serum can be collected, known as process fluids
(PF), which has proven successful in monitoring herds for the presence of porcine
reproductive and respiratory syndrome virus (PRRSV). Furthermore, air sampling has emerged as a novel technique to detect pathogens in various farming systems and
animal species. This method offers the advantage of obtaining diagnostic samples
without direct animal contact.
By employing these non-invasive sampling methods, swine producers can implement
effective surveillance strategies while maintaining animal welfare standards and
obtaining reliable diagnostic information.",
publisher = "Beograd : Fakultet veterinarske medicine, Univerzitet u Beogradu, Beograd : Veterinarska komora Srbije",
journal = "Veterinarski glasnik",
title = "Overview of non-invasive sampling methods used in intensive swine farming",
pages = "108-97",
number = "2",
volume = "77",
doi = "10.2298/VETGL230614004G"
}

Glišić, D., Veljović, L., Milovanović, B., Ninković, M., Maletić, J., Kureljušić, B.,& Milićević, V.. (2023). Overview of non-invasive sampling methods used in intensive swine farming. in Veterinarski glasnik
Beograd : Fakultet veterinarske medicine, Univerzitet u Beogradu., 77(2), 97-108.
https://doi.org/10.2298/VETGL230614004G

Glišić D, Veljović L, Milovanović B, Ninković M, Maletić J, Kureljušić B, Milićević V. Overview of non-invasive sampling methods used in intensive swine farming. in Veterinarski glasnik. 2023;77(2):97-108.
doi:10.2298/VETGL230614004G .

Glišić, Dimitrije, Veljović, Ljubiša, Milovanović, Bojan, Ninković, Milan, Maletić, Jelena, Kureljušić, Branislav, Milićević, Vesna, "Overview of non-invasive sampling methods used in intensive swine farming" in Veterinarski glasnik, 77, no. 2 (2023):97-108,
https://doi.org/10.2298/VETGL230614004G . .

TY  - JOUR
AU  - Milićević, Vesna
AU  - Glišić, Dimitrije
AU  - Veljović, Ljubiša
AU  - Maletić, Jelena
AU  - Kureljušić, Branislav
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/835
AB  - Rabies is a viral disease of the central nervous system of all warm-blooded animals
and one of the oldest and most important zoonoses. In the Republic of Serbia, rabies is controlled by compulsory vaccination of dogs and cats and oral vaccination of
wild carnivores, which has been implemented since 2010. In the period 2009–2018,
367 rabies cases were recorded. The last rabies case in Serbia was recorded in 2018
in a red fox (Vulpes vulpes), while the last case in dogs was diagnosed in 2011. This
study aimed to perform a retrospective phylogenetic analysis of G and N genes of
the rabies virus from Serbia from 2009 to 2018 to understand sources and pathways
of infection better and to enable molecular virus tracing in the future based on the
association of rabies virus genetic lineages with the geographic distribution. For the
phylogenetic analysis of the rabies virus, 14 historically positive brain samples of red
foxes from 2009 to 2018, collected in central Serbia, were used. All field strains from
Serbia were identified as classic rabies virus and grouped within the Cosmopolitan lineage. Phylogenetic analysis of N gene sequences revealed that 13 rabies virus strains
(92.9%) from Serbia belonged to the EE sub-lineage of isolates, while one virus (7.1%)
ON988027 from 2011 clustered together with isolates from the WE sub-lineage.
PB  - Wiley
T2  - Zoonoses and Public Health
T1  - Retrospective phylogenetic analysis of rabies virus G and N genes from Serbia
EP  - 704
IS  - 8
SP  - 699
VL  - 70
DO  - 10.1111/zph.13061
ER  - 

@article{
author = "Milićević, Vesna and Glišić, Dimitrije and Veljović, Ljubiša and Maletić, Jelena and Kureljušić, Branislav",
year = "2023",
abstract = "Rabies is a viral disease of the central nervous system of all warm-blooded animals
and one of the oldest and most important zoonoses. In the Republic of Serbia, rabies is controlled by compulsory vaccination of dogs and cats and oral vaccination of
wild carnivores, which has been implemented since 2010. In the period 2009–2018,
367 rabies cases were recorded. The last rabies case in Serbia was recorded in 2018
in a red fox (Vulpes vulpes), while the last case in dogs was diagnosed in 2011. This
study aimed to perform a retrospective phylogenetic analysis of G and N genes of
the rabies virus from Serbia from 2009 to 2018 to understand sources and pathways
of infection better and to enable molecular virus tracing in the future based on the
association of rabies virus genetic lineages with the geographic distribution. For the
phylogenetic analysis of the rabies virus, 14 historically positive brain samples of red
foxes from 2009 to 2018, collected in central Serbia, were used. All field strains from
Serbia were identified as classic rabies virus and grouped within the Cosmopolitan lineage. Phylogenetic analysis of N gene sequences revealed that 13 rabies virus strains
(92.9%) from Serbia belonged to the EE sub-lineage of isolates, while one virus (7.1%)
ON988027 from 2011 clustered together with isolates from the WE sub-lineage.",
publisher = "Wiley",
journal = "Zoonoses and Public Health",
title = "Retrospective phylogenetic analysis of rabies virus G and N genes from Serbia",
pages = "704-699",
number = "8",
volume = "70",
doi = "10.1111/zph.13061"
}

Milićević, V., Glišić, D., Veljović, L., Maletić, J.,& Kureljušić, B.. (2023). Retrospective phylogenetic analysis of rabies virus G and N genes from Serbia. in Zoonoses and Public Health
Wiley., 70(8), 699-704.
https://doi.org/10.1111/zph.13061

Milićević V, Glišić D, Veljović L, Maletić J, Kureljušić B. Retrospective phylogenetic analysis of rabies virus G and N genes from Serbia. in Zoonoses and Public Health. 2023;70(8):699-704.
doi:10.1111/zph.13061 .

Milićević, Vesna, Glišić, Dimitrije, Veljović, Ljubiša, Maletić, Jelena, Kureljušić, Branislav, "Retrospective phylogenetic analysis of rabies virus G and N genes from Serbia" in Zoonoses and Public Health, 70, no. 8 (2023):699-704,
https://doi.org/10.1111/zph.13061 . .

TY  - JOUR
AU  - Maletić, Jelena
AU  - Spalević, Ljiljana
AU  - Milićević, Vesna
AU  - Glišić, Dimitrije
AU  - Kureljušić, Branislav
AU  - Kureljušić, Jasna
AU  - Maletić, Milan
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/832
AB  - The implementation of biosecurity measures as the first line of defense is an essential prerequisite for preventing the occurrence and spread of infectious agents in flocks. Assessment of biosecurity measures on the farms was carried out during farm visits based on the application of an appropriate questionnaire whereby the farmer answered a number of questions regarding the implemented biosecurity measures. The study was performed via Biocheck.UGent online survey on 16 broiler farms in the region of the city of Belgrade. The capacity of farms was 25,000-100,000 broilers, placed in 2-4 houses, depending on the farm. Farm visits were made by the authors to confirm the correct answers to the online survey. Results showed that external biosecurity scores ranged from 57% to 93%, averaging 83.6%. Internal biosecurity score ranged from 48% to 98%, with an average score of 85.7%. The total score for the biosecurity assessment ranged from 56% to 93%, with an average score of 84.3%. The scores for subcategories varied between the farms. Removing manure and carcasses, a subcategory within the category of external biosecurity, had the lowest mean score (farms 9 and 10 had a score of 12%). Relatively low scores were also obtained for the number of steps of broilers depopulation (farm 9 had a score of 44%) and for the location of the farm (farms 2 and 3 had a score of 44%). Concerning internal biosecurity, the lowest score was obtained for the subcategory material and measures that they apply between compartments (farm 10 had a score of 29%). These findings should be useful for decision-makers and flock veterinarians and farmers to set feasible targets and sustainable biosecurity programs to improve biosecurity, the health status of the flock, and farm profitability.
AB  - Sprovođenje biosigurnosnih mera, kao prve linije odbrane, suštinski je preduslov za sprečavanje pojave i širenja infektivnih oboljenja kod živine. Procena biosigurnosnih mera na farmama izvršena je primenom odgovarajućeg upitnika, gde je farmer odgovorio na brojna pitanja u vezi sa sprovedenim biosigurnosnim merama. Istraživanje je sprovedeno putem onlajn ankete Biocheck.Ugent na 16 farmi brojlera u regionu grada Beograda. Kapacitet farmi je bio 25000-100000 brojlera, smeštenih u 2 - 4 objekta, u zavisnosti od farme. Rezultati su pokazali da se eksterna biosigurnost kretala od 57 % do 93 %, sa prosečnom ocenom od 83.6 %. Rezultat interne biosigurnosti se kretao od 48 % do 98 %, sa prosečnom ocenom od 85.7 %. Krajnji rezultat procene biosigurnosti kretao se od 56 % do 93 %, sa prosečnom ocenom od 84.3%. Rezultati za potkategorije su varirali između farmi. Uklanjanje stajnjaka i leševa, potkategorija u okviru kategorije eksterne biobezbednosti, imala je najnižu srednju ocenu (farme 9 i 10 su imale ocenu od 12 %). Niže ocene takođe su dobijene za potkategorije broj koraka depopulacije brojlera (farma 9 je imala ocenu 44 %) i za lokaciju farme (farme 2 i 3 su imale ocenu od 44 %). Što se tiče interne biosigurnosti, najniža ocena je dobijena za potkategoriju materijal i mere koje se primenjuju između odeljaka (farma 10 imala je ocenu od 29 %). Ovi nalazi trebalo bi da budu korisni donosiocima odluka i veterinarima i farmerima u postavljanju izvodljivih ciljeva i održivih biosigurnosnih programa, za poboljšanje biobezbednosti, zdravstvenog statusa jata i profitabilnosti farme.
PB  - Beograd : Fakultet veterinarske medicine, Univerzitet u Beogradu
T2  - Veterinarski glasnik
T1  - Assessment of biosecurity measures implemented on the broiler farms in the region of Belgrade city
T1  - Ocena biosigurnosnih mera implementiranih na farmama brojlera na beogradskom području
EP  - 136
IS  - 2
SP  - 125
VL  - 77
DO  - 10.2298/VETGL230403003M
ER  - 

@article{
author = "Maletić, Jelena and Spalević, Ljiljana and Milićević, Vesna and Glišić, Dimitrije and Kureljušić, Branislav and Kureljušić, Jasna and Maletić, Milan",
year = "2023",
abstract = "The implementation of biosecurity measures as the first line of defense is an essential prerequisite for preventing the occurrence and spread of infectious agents in flocks. Assessment of biosecurity measures on the farms was carried out during farm visits based on the application of an appropriate questionnaire whereby the farmer answered a number of questions regarding the implemented biosecurity measures. The study was performed via Biocheck.UGent online survey on 16 broiler farms in the region of the city of Belgrade. The capacity of farms was 25,000-100,000 broilers, placed in 2-4 houses, depending on the farm. Farm visits were made by the authors to confirm the correct answers to the online survey. Results showed that external biosecurity scores ranged from 57% to 93%, averaging 83.6%. Internal biosecurity score ranged from 48% to 98%, with an average score of 85.7%. The total score for the biosecurity assessment ranged from 56% to 93%, with an average score of 84.3%. The scores for subcategories varied between the farms. Removing manure and carcasses, a subcategory within the category of external biosecurity, had the lowest mean score (farms 9 and 10 had a score of 12%). Relatively low scores were also obtained for the number of steps of broilers depopulation (farm 9 had a score of 44%) and for the location of the farm (farms 2 and 3 had a score of 44%). Concerning internal biosecurity, the lowest score was obtained for the subcategory material and measures that they apply between compartments (farm 10 had a score of 29%). These findings should be useful for decision-makers and flock veterinarians and farmers to set feasible targets and sustainable biosecurity programs to improve biosecurity, the health status of the flock, and farm profitability., Sprovođenje biosigurnosnih mera, kao prve linije odbrane, suštinski je preduslov za sprečavanje pojave i širenja infektivnih oboljenja kod živine. Procena biosigurnosnih mera na farmama izvršena je primenom odgovarajućeg upitnika, gde je farmer odgovorio na brojna pitanja u vezi sa sprovedenim biosigurnosnim merama. Istraživanje je sprovedeno putem onlajn ankete Biocheck.Ugent na 16 farmi brojlera u regionu grada Beograda. Kapacitet farmi je bio 25000-100000 brojlera, smeštenih u 2 - 4 objekta, u zavisnosti od farme. Rezultati su pokazali da se eksterna biosigurnost kretala od 57 % do 93 %, sa prosečnom ocenom od 83.6 %. Rezultat interne biosigurnosti se kretao od 48 % do 98 %, sa prosečnom ocenom od 85.7 %. Krajnji rezultat procene biosigurnosti kretao se od 56 % do 93 %, sa prosečnom ocenom od 84.3%. Rezultati za potkategorije su varirali između farmi. Uklanjanje stajnjaka i leševa, potkategorija u okviru kategorije eksterne biobezbednosti, imala je najnižu srednju ocenu (farme 9 i 10 su imale ocenu od 12 %). Niže ocene takođe su dobijene za potkategorije broj koraka depopulacije brojlera (farma 9 je imala ocenu 44 %) i za lokaciju farme (farme 2 i 3 su imale ocenu od 44 %). Što se tiče interne biosigurnosti, najniža ocena je dobijena za potkategoriju materijal i mere koje se primenjuju između odeljaka (farma 10 imala je ocenu od 29 %). Ovi nalazi trebalo bi da budu korisni donosiocima odluka i veterinarima i farmerima u postavljanju izvodljivih ciljeva i održivih biosigurnosnih programa, za poboljšanje biobezbednosti, zdravstvenog statusa jata i profitabilnosti farme.",
publisher = "Beograd : Fakultet veterinarske medicine, Univerzitet u Beogradu",
journal = "Veterinarski glasnik",
title = "Assessment of biosecurity measures implemented on the broiler farms in the region of Belgrade city, Ocena biosigurnosnih mera implementiranih na farmama brojlera na beogradskom području",
pages = "136-125",
number = "2",
volume = "77",
doi = "10.2298/VETGL230403003M"
}

Maletić, J., Spalević, L., Milićević, V., Glišić, D., Kureljušić, B., Kureljušić, J.,& Maletić, M.. (2023). Assessment of biosecurity measures implemented on the broiler farms in the region of Belgrade city. in Veterinarski glasnik
Beograd : Fakultet veterinarske medicine, Univerzitet u Beogradu., 77(2), 125-136.
https://doi.org/10.2298/VETGL230403003M

Maletić J, Spalević L, Milićević V, Glišić D, Kureljušić B, Kureljušić J, Maletić M. Assessment of biosecurity measures implemented on the broiler farms in the region of Belgrade city. in Veterinarski glasnik. 2023;77(2):125-136.
doi:10.2298/VETGL230403003M .

Maletić, Jelena, Spalević, Ljiljana, Milićević, Vesna, Glišić, Dimitrije, Kureljušić, Branislav, Kureljušić, Jasna, Maletić, Milan, "Assessment of biosecurity measures implemented on the broiler farms in the region of Belgrade city" in Veterinarski glasnik, 77, no. 2 (2023):125-136,
https://doi.org/10.2298/VETGL230403003M . .

TY  - CONF
AU  - Savić, Božidar
AU  - Kureljušić, Branislav
AU  - Milićević, Vesna
AU  - Jezdimirović, Nemanja
AU  - Milovanović, Bojan
AU  - Zdravković, Nemanja
AU  - Stevančević, Dimitrije
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/802
AB  - Porcine reproductive and respiratory syndrome virus CPRRSV) is the causative agent of a devastating pig disease worldwide, which is endemic in many countries, including the Serbia. Only limited epidemiological or molecular studies has been published on circulating PRRS-viruses in the Serbia since the early nineties. Therefore, the aim of this study is to investigate the genetic diversity of circulating PRRS-viruses in Serbia in the last ten years, by comparing the nucleotid€ sequences obtained for ORF5, wlth the ORF5 sequences of PRRSV 1 subtype 1.
PB  - ECPHM
C3  - Proceedings : ESPHM : 14th European symposium of porcine health management
T1  - Genetic diversity of the orf5 gene of type 1 porcine reproductive and respiratory syndrome virus isolates in Serbian swine herds from 2012 to 2022
EP  - 323
SP  - 323
ER  - 

@conference{
author = "Savić, Božidar and Kureljušić, Branislav and Milićević, Vesna and Jezdimirović, Nemanja and Milovanović, Bojan and Zdravković, Nemanja and Stevančević, Dimitrije",
year = "2023",
abstract = "Porcine reproductive and respiratory syndrome virus CPRRSV) is the causative agent of a devastating pig disease worldwide, which is endemic in many countries, including the Serbia. Only limited epidemiological or molecular studies has been published on circulating PRRS-viruses in the Serbia since the early nineties. Therefore, the aim of this study is to investigate the genetic diversity of circulating PRRS-viruses in Serbia in the last ten years, by comparing the nucleotid€ sequences obtained for ORF5, wlth the ORF5 sequences of PRRSV 1 subtype 1.",
publisher = "ECPHM",
journal = "Proceedings : ESPHM : 14th European symposium of porcine health management",
title = "Genetic diversity of the orf5 gene of type 1 porcine reproductive and respiratory syndrome virus isolates in Serbian swine herds from 2012 to 2022",
pages = "323-323"
}

Savić, B., Kureljušić, B., Milićević, V., Jezdimirović, N., Milovanović, B., Zdravković, N.,& Stevančević, D.. (2023). Genetic diversity of the orf5 gene of type 1 porcine reproductive and respiratory syndrome virus isolates in Serbian swine herds from 2012 to 2022. in Proceedings : ESPHM : 14th European symposium of porcine health management
ECPHM., 323-323.

Savić B, Kureljušić B, Milićević V, Jezdimirović N, Milovanović B, Zdravković N, Stevančević D. Genetic diversity of the orf5 gene of type 1 porcine reproductive and respiratory syndrome virus isolates in Serbian swine herds from 2012 to 2022. in Proceedings : ESPHM : 14th European symposium of porcine health management. 2023;:323-323..

Savić, Božidar, Kureljušić, Branislav, Milićević, Vesna, Jezdimirović, Nemanja, Milovanović, Bojan, Zdravković, Nemanja, Stevančević, Dimitrije, "Genetic diversity of the orf5 gene of type 1 porcine reproductive and respiratory syndrome virus isolates in Serbian swine herds from 2012 to 2022" in Proceedings : ESPHM : 14th European symposium of porcine health management (2023):323-323.

TY  - CONF
AU  - Milićević, Vesna
AU  - Glišić, Dimitrije
AU  - Milovanović, Bojan
AU  - Veljović, Ljubiša
AU  - Maletić, Jelena
AU  - Kureljušić, Branislav
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/803
AB  - Non-invasive sampling is increasingly used in domestic and wild animals. Traditional sampling methods are labour, time-consuming, and require trained personnel. Oral fluid is an alternative sample that can replace nasal swabs. Air sampling with special device and testing represents the newest method of non-invasive sampling, which is particularly useful and validated when testing pigs. Udder skin sampling is a new sampling approach that provides insight into litters of pigs before weaning, containing their nasal and oral secretions.
PB  - ECPHM
C3  - Proceedings : ESPHM : 14th European symposium of porcine health management
T1  - Porcine viral diseases can be successfully  monitored using non-invasive sampling strategy
EP  - 317
SP  - 317
ER  - 

@conference{
author = "Milićević, Vesna and Glišić, Dimitrije and Milovanović, Bojan and Veljović, Ljubiša and Maletić, Jelena and Kureljušić, Branislav",
year = "2023",
abstract = "Non-invasive sampling is increasingly used in domestic and wild animals. Traditional sampling methods are labour, time-consuming, and require trained personnel. Oral fluid is an alternative sample that can replace nasal swabs. Air sampling with special device and testing represents the newest method of non-invasive sampling, which is particularly useful and validated when testing pigs. Udder skin sampling is a new sampling approach that provides insight into litters of pigs before weaning, containing their nasal and oral secretions.",
publisher = "ECPHM",
journal = "Proceedings : ESPHM : 14th European symposium of porcine health management",
title = "Porcine viral diseases can be successfully  monitored using non-invasive sampling strategy",
pages = "317-317"
}

Milićević, V., Glišić, D., Milovanović, B., Veljović, L., Maletić, J.,& Kureljušić, B.. (2023). Porcine viral diseases can be successfully  monitored using non-invasive sampling strategy. in Proceedings : ESPHM : 14th European symposium of porcine health management
ECPHM., 317-317.

Milićević V, Glišić D, Milovanović B, Veljović L, Maletić J, Kureljušić B. Porcine viral diseases can be successfully  monitored using non-invasive sampling strategy. in Proceedings : ESPHM : 14th European symposium of porcine health management. 2023;:317-317..

Milićević, Vesna, Glišić, Dimitrije, Milovanović, Bojan, Veljović, Ljubiša, Maletić, Jelena, Kureljušić, Branislav, "Porcine viral diseases can be successfully  monitored using non-invasive sampling strategy" in Proceedings : ESPHM : 14th European symposium of porcine health management (2023):317-317.

TY  - CONF
AU  - Kureljušić, Branislav
AU  - Milićević, Vesna
AU  - Milovanović, Bojan
AU  - Glišić, Dimitrije
AU  - Maletić, Jelena
AU  - Jezdimirović, Nemanja
AU  - Kureljušić, Jasna
AU  - Prodanov Radulović, Jasna
AU  - Savić, Božidar
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/804
AB  - Biosecurity ls of paramount Importance to prevent the introduction and spread of pathogens in pig farms. Applied biosecurity measures and efficient on-farm management protect pigs from different infectious diseases. African swine fever CASF) is a severe, viral disease of domestic pigs and wild boar. After the occurrence in 2007 in Georgia, the disease gradually spread throughout Europe. The first case of ASF in Serbia was confirmed on July 30, 2019.
PB  - ECPHM
C3  - Proceedings : ESPHM : 14th European symposium of porcine health management
T1  - Assessment of biosecurity measures in the Serbian backyards where the first African swine fever cases were confirmed
EP  - 253
SP  - 253
ER  - 

@conference{
author = "Kureljušić, Branislav and Milićević, Vesna and Milovanović, Bojan and Glišić, Dimitrije and Maletić, Jelena and Jezdimirović, Nemanja and Kureljušić, Jasna and Prodanov Radulović, Jasna and Savić, Božidar",
year = "2023",
abstract = "Biosecurity ls of paramount Importance to prevent the introduction and spread of pathogens in pig farms. Applied biosecurity measures and efficient on-farm management protect pigs from different infectious diseases. African swine fever CASF) is a severe, viral disease of domestic pigs and wild boar. After the occurrence in 2007 in Georgia, the disease gradually spread throughout Europe. The first case of ASF in Serbia was confirmed on July 30, 2019.",
publisher = "ECPHM",
journal = "Proceedings : ESPHM : 14th European symposium of porcine health management",
title = "Assessment of biosecurity measures in the Serbian backyards where the first African swine fever cases were confirmed",
pages = "253-253"
}

Kureljušić, B., Milićević, V., Milovanović, B., Glišić, D., Maletić, J., Jezdimirović, N., Kureljušić, J., Prodanov Radulović, J.,& Savić, B.. (2023). Assessment of biosecurity measures in the Serbian backyards where the first African swine fever cases were confirmed. in Proceedings : ESPHM : 14th European symposium of porcine health management
ECPHM., 253-253.

Kureljušić B, Milićević V, Milovanović B, Glišić D, Maletić J, Jezdimirović N, Kureljušić J, Prodanov Radulović J, Savić B. Assessment of biosecurity measures in the Serbian backyards where the first African swine fever cases were confirmed. in Proceedings : ESPHM : 14th European symposium of porcine health management. 2023;:253-253..

Kureljušić, Branislav, Milićević, Vesna, Milovanović, Bojan, Glišić, Dimitrije, Maletić, Jelena, Jezdimirović, Nemanja, Kureljušić, Jasna, Prodanov Radulović, Jasna, Savić, Božidar, "Assessment of biosecurity measures in the Serbian backyards where the first African swine fever cases were confirmed" in Proceedings : ESPHM : 14th European symposium of porcine health management (2023):253-253.

TY  - JOUR
AU  - Milićević, Vesna
AU  - Glišić, Dimitrije
AU  - Veljović, Ljubiša
AU  - Vasić, Ana
AU  - Milovanović, Bojan
AU  - Kureljušić, Branislav
AU  - Paunović, Milan
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/777
AB  - Parvoviruses are among the major animal pathogens that can cause considerable health disorders ranging from subclinical
to lethal in domestic and wild animals. Golden jackal (Canis aureus), an expanding European species, is a reservoir of many
pathogens, including vector-borne diseases and zoonoses. Given the importance of parvovirus infections in dogs and cats,
this study aimed to unfold the virus prevalence and molecular characterisation in the golden jackal population in Serbia.
The spleen samples from 68 hunted jackals during 2022/2023 were tested for the VP2-specific genome region of Protoparvovirus
carnivoran 1 by PCR. BLAST analysis of partial VP2 sequences obtained from three animals (4.4%) revealed the
highest similarity to Protoparvovirus carnivoran 1, genogroup Feline panleukopenia virus, which is the second report on
FPV infection in jackals. Based on specific amino acid residues within partial VP2, the jackals’ Protoparvovirus carnivoran
1 was also classified as FPV. One jackal’s strain showed two synonymous mutations at positions 699 and 1167. Although
species cross-transmission could not be established, jackals’ health should be maintained by preventing the transmission
of viruses to native species and vice versa. Although jackals are considered pests, their role as natural cleaners is of greater
importance. Therefore, further monitoring of their health is needed to understand the influence of infectious diseases on
population dynamics and to determine the relationship between domestic predators and jackals and the direction of crossspecies
transmission.
PB  - Springer
T2  - Veterinary Research Communications
T1  - Protoparvovirus carnivoran 1 infection of golden jackals Canis aureus in Serbia
DO  - 10.1007/s11259-023-10249-0
ER  - 

@article{
author = "Milićević, Vesna and Glišić, Dimitrije and Veljović, Ljubiša and Vasić, Ana and Milovanović, Bojan and Kureljušić, Branislav and Paunović, Milan",
year = "2023",
abstract = "Parvoviruses are among the major animal pathogens that can cause considerable health disorders ranging from subclinical
to lethal in domestic and wild animals. Golden jackal (Canis aureus), an expanding European species, is a reservoir of many
pathogens, including vector-borne diseases and zoonoses. Given the importance of parvovirus infections in dogs and cats,
this study aimed to unfold the virus prevalence and molecular characterisation in the golden jackal population in Serbia.
The spleen samples from 68 hunted jackals during 2022/2023 were tested for the VP2-specific genome region of Protoparvovirus
carnivoran 1 by PCR. BLAST analysis of partial VP2 sequences obtained from three animals (4.4%) revealed the
highest similarity to Protoparvovirus carnivoran 1, genogroup Feline panleukopenia virus, which is the second report on
FPV infection in jackals. Based on specific amino acid residues within partial VP2, the jackals’ Protoparvovirus carnivoran
1 was also classified as FPV. One jackal’s strain showed two synonymous mutations at positions 699 and 1167. Although
species cross-transmission could not be established, jackals’ health should be maintained by preventing the transmission
of viruses to native species and vice versa. Although jackals are considered pests, their role as natural cleaners is of greater
importance. Therefore, further monitoring of their health is needed to understand the influence of infectious diseases on
population dynamics and to determine the relationship between domestic predators and jackals and the direction of crossspecies
transmission.",
publisher = "Springer",
journal = "Veterinary Research Communications",
title = "Protoparvovirus carnivoran 1 infection of golden jackals Canis aureus in Serbia",
doi = "10.1007/s11259-023-10249-0"
}

Milićević, V., Glišić, D., Veljović, L., Vasić, A., Milovanović, B., Kureljušić, B.,& Paunović, M.. (2023). Protoparvovirus carnivoran 1 infection of golden jackals Canis aureus in Serbia. in Veterinary Research Communications
Springer..
https://doi.org/10.1007/s11259-023-10249-0

Milićević V, Glišić D, Veljović L, Vasić A, Milovanović B, Kureljušić B, Paunović M. Protoparvovirus carnivoran 1 infection of golden jackals Canis aureus in Serbia. in Veterinary Research Communications. 2023;.
doi:10.1007/s11259-023-10249-0 .

Milićević, Vesna, Glišić, Dimitrije, Veljović, Ljubiša, Vasić, Ana, Milovanović, Bojan, Kureljušić, Branislav, Paunović, Milan, "Protoparvovirus carnivoran 1 infection of golden jackals Canis aureus in Serbia" in Veterinary Research Communications (2023),
https://doi.org/10.1007/s11259-023-10249-0 . .

TY  - JOUR
AU  - Milićević, Vesna
AU  - Glišić, Dimitrije
AU  - Zurovac Sapundžić, Zorana
AU  - Milovanović, Bojan
AU  - Maletić, Jelena
AU  - Jezdimirović, Nemanja
AU  - Kureljušić, Branislav
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/773
AB  - Contrary to pig farming in developedWestern countries, in a large part of the world, pigs
are still traditionally kept in small backyard farms, usually for family needs. Their main characteristics
are low biosecurity, swill feeding, natural breeding and uncontrolled trade. Given the high number
of backyard farms in Serbia and the risk they are thought to pose to intensive pig farming, the main
aim of this study was to evaluate the prevalence of major viral diseases of swine among traditionally
kept pigs in small holdings with low biosecurity. For this investigation, 222 serum samples from
69 backyard holdings were randomly selected and tested for antibodies to Porcine Reproductive and
Respiratory Syndrome virus (PRRSV), Aujeszky’s disease virus (ADV), Porcine Parvovirus (PPV)
and Swine influenza Virus (SIV) by enzyme-linked immunosorbent assay (ELISA). The herd-level
seroprevalence of PRRS, Aujeszky’s disease and PPV was 2.9%, 27.5% and 37.7%, respectively. Swine
influenza seroconversion was not confirmed in any of the tested holdings. Despite widely distributed
PPV and AD in backyard farms in Serbia, almost 50% of them are still negative for all the tested
diseases. The backyard farms must be monitored, and owners must be educated as their role in
eradication programs and obtaining country-free status may be crucial.
PB  - MDPI
T2  - Animals
T1  - Seroprevalence of viral enzootic diseases in swine backyard farms in Serbia
SP  - 3409
VL  - 13
DO  - 10.3390/ ani13213409
ER  - 

@article{
author = "Milićević, Vesna and Glišić, Dimitrije and Zurovac Sapundžić, Zorana and Milovanović, Bojan and Maletić, Jelena and Jezdimirović, Nemanja and Kureljušić, Branislav",
year = "2023",
abstract = "Contrary to pig farming in developedWestern countries, in a large part of the world, pigs
are still traditionally kept in small backyard farms, usually for family needs. Their main characteristics
are low biosecurity, swill feeding, natural breeding and uncontrolled trade. Given the high number
of backyard farms in Serbia and the risk they are thought to pose to intensive pig farming, the main
aim of this study was to evaluate the prevalence of major viral diseases of swine among traditionally
kept pigs in small holdings with low biosecurity. For this investigation, 222 serum samples from
69 backyard holdings were randomly selected and tested for antibodies to Porcine Reproductive and
Respiratory Syndrome virus (PRRSV), Aujeszky’s disease virus (ADV), Porcine Parvovirus (PPV)
and Swine influenza Virus (SIV) by enzyme-linked immunosorbent assay (ELISA). The herd-level
seroprevalence of PRRS, Aujeszky’s disease and PPV was 2.9%, 27.5% and 37.7%, respectively. Swine
influenza seroconversion was not confirmed in any of the tested holdings. Despite widely distributed
PPV and AD in backyard farms in Serbia, almost 50% of them are still negative for all the tested
diseases. The backyard farms must be monitored, and owners must be educated as their role in
eradication programs and obtaining country-free status may be crucial.",
publisher = "MDPI",
journal = "Animals",
title = "Seroprevalence of viral enzootic diseases in swine backyard farms in Serbia",
pages = "3409",
volume = "13",
doi = "10.3390/ ani13213409"
}

Milićević, V., Glišić, D., Zurovac Sapundžić, Z., Milovanović, B., Maletić, J., Jezdimirović, N.,& Kureljušić, B.. (2023). Seroprevalence of viral enzootic diseases in swine backyard farms in Serbia. in Animals
MDPI., 13, 3409.
https://doi.org/10.3390/ ani13213409

Milićević V, Glišić D, Zurovac Sapundžić Z, Milovanović B, Maletić J, Jezdimirović N, Kureljušić B. Seroprevalence of viral enzootic diseases in swine backyard farms in Serbia. in Animals. 2023;13:3409.
doi:10.3390/ ani13213409 .

Milićević, Vesna, Glišić, Dimitrije, Zurovac Sapundžić, Zorana, Milovanović, Bojan, Maletić, Jelena, Jezdimirović, Nemanja, Kureljušić, Branislav, "Seroprevalence of viral enzootic diseases in swine backyard farms in Serbia" in Animals, 13 (2023):3409,
https://doi.org/10.3390/ ani13213409 . .

TY  - CONF
AU  - Milićević, Vesna
AU  - Kureljušić, Branislav
AU  - Glišić, Dimitrije
AU  - Milovanović, Bojan
AU  - Veljović, Ljubiša
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/768
AB  - Foot-and-mouth disease (FMD) is a highly contagious viral disease of domestic
and wild ungulates and one of the most important diseases of animals in international
trade because it spreads very quickly and causes significant economic losses. In Africa,
Asia, the Middle East and a limited area of South America, FMD is an enzootic disease
and is controlled by vaccination. It is estimated that 77% of the world's livestock
population is affected by FMD (WOAH, 2022). Countries that are free of FMD without
vaccination are constantly at risk and invest heavily in preventing the introduction of
the virus. The causative agent of this disease is the FMD virus, which belongs to the
genus Aphtovirus and the family Picornaviridae. The virus is relatively resistant and
can survive in contaminated materials and the environment for several months under
ideal conditions. There are 7 serotypes and more than 60 subtypes of the FMD virus.
Immunity against one type of virus does not protect the animal from other serotypes
or subtypes.
The last cases of FMD in Europe were recorded in 2001 in the United Kingdom
(UK) and in 2011 in Bulgaria. FMD in the UK was registered on 2000 farms, as a result
of which about seven million animals were killed (Sutmoller et al., 2003). In Bulgaria,
the first case was detected in a wild boar on the border with Turkey, and then the
disease spread to domestic animals, cattle, sheep, goats and pigs on 11 farms. 2230 animals were killed in Bulgaria. Although sporadic cases of the disease were later
detected in wild game, it has not been proven that the FMD virus can be maintained in
wild animal populations (Elbers et al., 2003). The most common mechanism of the
spread of FMD is through direct contact, which can take the form of mechanical
transmission of the virus from infected to healthy animals through the skin and
mucous membranes or by aerosol and even by wind. The most important source of
infection is the contents of the vesicle, but the virus is also excreted in all
secretions/excreta, as well as in exhaled air. Cattle excrete less virus in their breath,
but, unlike pigs, they are very susceptible to infection through the respiratory route.
The minimum infectious dose of airborne virus for ruminants is only 10 TCID50, while
for pigs it is 103 TCID50 (Alekandersen et al., 2003). On the other hand, pigs are
considered an amplifier of the virus because they produce and excrete the most virus,
and therefore represent the greatest risk of spreading the disease. Depending on the
wind speed, infected pigs are a source of infection especially for cattle for miles
around. Such spread was noted in the UK when the disease was transferred from a pig
farm on the Isle of Wight to an area in northern France (Donaldson et al., 1982).
In addition to the pig phenomenon, a persistent form of infection in the
nasopharyngeal epithelium and lymphatic tissue is very important epizootiologically.
Persistent infection is defined as the presence of an infectious virus in the esophagealpharyngeal
(OP) fluid more than 15 or 21 days after infection in vaccinated or infected
cattle. The maximum duration of persistent infection in cattle is 3.5 years, sheep 9
months and goats 4 months (Alekandersen et al., 2003).
Clinically, the disease is characterized by a high body temperature (~
the appearance of vesicles in the oral cavity, feet and teats. After the bursting of the
vesicles, the processes become extremely painful, as a result of which the animals
cannot eat and avoid standing up if they have changes in their hooves. Most of the
affected animals recover, but with significant consequences, especially in milk
production. It is interesting that in young animals, sudden death can be the only sign
due to multifocal myocarditis that occurs before the appearance of vesicles. The
disease in sheep is mostly inapparent, which is why sheep are the species of choice for
serological surveillance of FMD.
In relation to the distribution of viruses, there are 7 enzootic areas (pools)
characterized by virus genotypes that circulate and develop independently within the
area. However, every year, the virus jumps from one area to another, usually through
illegal animal traffic. A very worrying epizootic event was recorded in early 2023 with
the emergence of SAT2/KSIV in Iraq, Jordan and Turkey (ADIS, 2023). The SAT2
epizootic was registered in three districts in Turkey on March 3, 2023, in the east of
the country. Out of 4944 susceptible animals, in all three districts, 7 clinical cases and
no deaths were determined. Analyzes of the nucleotide sequences of viruses from
these areas confirmed that the epizootics were caused by viruses of the SAT2/KSIV
topotype closely related to the viruses from Ethiopia in 2022. Considering that this is
the first occurrence of SAT2 in the mentioned countries where the population is fully
received, there is a justified concern from the rapid spread to other countries in the
region, the buffer zone without FMD in Thrace, as well as further west.
Turkey is the bridge that connects Europe and Asia and as such is very important
epizootiologically. The mass movements of animals in Turkey are always in the
direction of the west, towards the Marmara region and further towards Europe.
Foot and mouth disease is one of the most difficult animal diseases to control.
Different strategies can be applied to control FMD including mass vaccination as is the
case in Turkey.
Traditional measures to prevent the spread are the prohibition of traffic and trade
in animals and animal products from infected areas. Strict control at the border is a
crucial tactical measure because it has been shown that the legal and illegal movement
of animals contributes to the spread of diseases. In case of disease, the "stamping out"
method is applied, with the possibility of ring vaccination.
FMD is always a current topic because of its disastrous consequences in livestock
farming. In the future, due to rapid genetic change among serotypes, the virus will
continue to evolve and cause epizootics in enzootic areas. Therefore, trained
veterinary staff, good infrastructure, trained management and rapid diagnostic testing
in well-equipped laboratories must be ensured before the emergence of SIS. In
addition, the existence of an effective monitoring and surveillance system is crucial.
Serbia is a country free from FMD. Every year, for the purpose of confirming the free
status, surveillance is carried out by serological testing of a representative number of samples, Legislatively, SIŠ is regulated by the Ruleboook on establishing measures for
early detection, diagnosis, prevention of the spread, suppression and eradication of
foot-and-mouth disease ("Official Gazette of the RS", no. 15/2010) and the Rulebook
on establishing a crisis management plan (" Official Gazette of the RS", No. 90/2015)
PB  - Beograd : Srpsko veterinarsko društvo
C3  - 34. Savetovanje veterinara Srbije, Zlatibor, 7-10. septembar 2023
T1  - Slinavka i šap - bolest koja nam stalno preti
T1  - Foot and mouth disease - constantly threatening disease
EP  - 20
SP  - 16
ER  - 

@conference{
author = "Milićević, Vesna and Kureljušić, Branislav and Glišić, Dimitrije and Milovanović, Bojan and Veljović, Ljubiša",
year = "2023",
abstract = "Foot-and-mouth disease (FMD) is a highly contagious viral disease of domestic
and wild ungulates and one of the most important diseases of animals in international
trade because it spreads very quickly and causes significant economic losses. In Africa,
Asia, the Middle East and a limited area of South America, FMD is an enzootic disease
and is controlled by vaccination. It is estimated that 77% of the world's livestock
population is affected by FMD (WOAH, 2022). Countries that are free of FMD without
vaccination are constantly at risk and invest heavily in preventing the introduction of
the virus. The causative agent of this disease is the FMD virus, which belongs to the
genus Aphtovirus and the family Picornaviridae. The virus is relatively resistant and
can survive in contaminated materials and the environment for several months under
ideal conditions. There are 7 serotypes and more than 60 subtypes of the FMD virus.
Immunity against one type of virus does not protect the animal from other serotypes
or subtypes.
The last cases of FMD in Europe were recorded in 2001 in the United Kingdom
(UK) and in 2011 in Bulgaria. FMD in the UK was registered on 2000 farms, as a result
of which about seven million animals were killed (Sutmoller et al., 2003). In Bulgaria,
the first case was detected in a wild boar on the border with Turkey, and then the
disease spread to domestic animals, cattle, sheep, goats and pigs on 11 farms. 2230 animals were killed in Bulgaria. Although sporadic cases of the disease were later
detected in wild game, it has not been proven that the FMD virus can be maintained in
wild animal populations (Elbers et al., 2003). The most common mechanism of the
spread of FMD is through direct contact, which can take the form of mechanical
transmission of the virus from infected to healthy animals through the skin and
mucous membranes or by aerosol and even by wind. The most important source of
infection is the contents of the vesicle, but the virus is also excreted in all
secretions/excreta, as well as in exhaled air. Cattle excrete less virus in their breath,
but, unlike pigs, they are very susceptible to infection through the respiratory route.
The minimum infectious dose of airborne virus for ruminants is only 10 TCID50, while
for pigs it is 103 TCID50 (Alekandersen et al., 2003). On the other hand, pigs are
considered an amplifier of the virus because they produce and excrete the most virus,
and therefore represent the greatest risk of spreading the disease. Depending on the
wind speed, infected pigs are a source of infection especially for cattle for miles
around. Such spread was noted in the UK when the disease was transferred from a pig
farm on the Isle of Wight to an area in northern France (Donaldson et al., 1982).
In addition to the pig phenomenon, a persistent form of infection in the
nasopharyngeal epithelium and lymphatic tissue is very important epizootiologically.
Persistent infection is defined as the presence of an infectious virus in the esophagealpharyngeal
(OP) fluid more than 15 or 21 days after infection in vaccinated or infected
cattle. The maximum duration of persistent infection in cattle is 3.5 years, sheep 9
months and goats 4 months (Alekandersen et al., 2003).
Clinically, the disease is characterized by a high body temperature (~
the appearance of vesicles in the oral cavity, feet and teats. After the bursting of the
vesicles, the processes become extremely painful, as a result of which the animals
cannot eat and avoid standing up if they have changes in their hooves. Most of the
affected animals recover, but with significant consequences, especially in milk
production. It is interesting that in young animals, sudden death can be the only sign
due to multifocal myocarditis that occurs before the appearance of vesicles. The
disease in sheep is mostly inapparent, which is why sheep are the species of choice for
serological surveillance of FMD.
In relation to the distribution of viruses, there are 7 enzootic areas (pools)
characterized by virus genotypes that circulate and develop independently within the
area. However, every year, the virus jumps from one area to another, usually through
illegal animal traffic. A very worrying epizootic event was recorded in early 2023 with
the emergence of SAT2/KSIV in Iraq, Jordan and Turkey (ADIS, 2023). The SAT2
epizootic was registered in three districts in Turkey on March 3, 2023, in the east of
the country. Out of 4944 susceptible animals, in all three districts, 7 clinical cases and
no deaths were determined. Analyzes of the nucleotide sequences of viruses from
these areas confirmed that the epizootics were caused by viruses of the SAT2/KSIV
topotype closely related to the viruses from Ethiopia in 2022. Considering that this is
the first occurrence of SAT2 in the mentioned countries where the population is fully
received, there is a justified concern from the rapid spread to other countries in the
region, the buffer zone without FMD in Thrace, as well as further west.
Turkey is the bridge that connects Europe and Asia and as such is very important
epizootiologically. The mass movements of animals in Turkey are always in the
direction of the west, towards the Marmara region and further towards Europe.
Foot and mouth disease is one of the most difficult animal diseases to control.
Different strategies can be applied to control FMD including mass vaccination as is the
case in Turkey.
Traditional measures to prevent the spread are the prohibition of traffic and trade
in animals and animal products from infected areas. Strict control at the border is a
crucial tactical measure because it has been shown that the legal and illegal movement
of animals contributes to the spread of diseases. In case of disease, the "stamping out"
method is applied, with the possibility of ring vaccination.
FMD is always a current topic because of its disastrous consequences in livestock
farming. In the future, due to rapid genetic change among serotypes, the virus will
continue to evolve and cause epizootics in enzootic areas. Therefore, trained
veterinary staff, good infrastructure, trained management and rapid diagnostic testing
in well-equipped laboratories must be ensured before the emergence of SIS. In
addition, the existence of an effective monitoring and surveillance system is crucial.
Serbia is a country free from FMD. Every year, for the purpose of confirming the free
status, surveillance is carried out by serological testing of a representative number of samples, Legislatively, SIŠ is regulated by the Ruleboook on establishing measures for
early detection, diagnosis, prevention of the spread, suppression and eradication of
foot-and-mouth disease ("Official Gazette of the RS", no. 15/2010) and the Rulebook
on establishing a crisis management plan (" Official Gazette of the RS", No. 90/2015)",
publisher = "Beograd : Srpsko veterinarsko društvo",
journal = "34. Savetovanje veterinara Srbije, Zlatibor, 7-10. septembar 2023",
title = "Slinavka i šap - bolest koja nam stalno preti, Foot and mouth disease - constantly threatening disease",
pages = "20-16"
}

Milićević, V., Kureljušić, B., Glišić, D., Milovanović, B.,& Veljović, L.. (2023). Slinavka i šap - bolest koja nam stalno preti. in 34. Savetovanje veterinara Srbije, Zlatibor, 7-10. septembar 2023
Beograd : Srpsko veterinarsko društvo., 16-20.

Milićević V, Kureljušić B, Glišić D, Milovanović B, Veljović L. Slinavka i šap - bolest koja nam stalno preti. in 34. Savetovanje veterinara Srbije, Zlatibor, 7-10. septembar 2023. 2023;:16-20..

Milićević, Vesna, Kureljušić, Branislav, Glišić, Dimitrije, Milovanović, Bojan, Veljović, Ljubiša, "Slinavka i šap - bolest koja nam stalno preti" in 34. Savetovanje veterinara Srbije, Zlatibor, 7-10. septembar 2023 (2023):16-20.

TY  - CONF
AU  - Maletić, Jelena
AU  - Kureljušić, Jasna
AU  - Milovanović, Bojan
AU  - Milićević, Vesna
AU  - Radosavljevic, Vladimir
AU  - Spalević, Ljiljana
AU  - Kureljušić, Branislav
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/551
AB  - Biosigurnost podrazumeva primenu određenih preventivnih mera na farmi u
cilju smanjenja mogućnosti unošenja i širenja patogenih mikroorganizama. Biosigurnost može biti spoljašnja (eksterna) i unutrašnja (interna). Procena biosigurnosti predstavlja kvalitativno ili kvantitativno određivanje nivoa biosigurnosti na
farmama. Istraživanje je sprovedeno na dve brojlerske farme na kojima je procena nivoa biosigurnosti sprovedena upotrebom on-lajn upitnika – Biocheck.Ugent.
Ukupna ocena biosigurnosti na farmi 1 bila je viša za 22% u odnosu na ocenu za
farmu 2. Ocena eksterne biosigurnosti na farmi 1 je za 27% viša u odnosu na ocenu za farmu 2, dok su se ocene za internu biosigurnost razlikovale za 8%. Takođe,
utvrđno je da su ocena eksterne biosigurnosti, kao i ukupna ocena za biosigurnosne mere koje se sprovode na farmi 2, niže u odnosu na svetski prosek dobijen iz
baze podataka Biochek.UGent-a. Dobijene ocene za potkategorije, razlikovale su
se između farmi. Najniže ocene za eksternu biosigurnost dobile su potkategorije
„uklanjanje stajnjaka i leševa” (5/100), „snabdevanje hranom i vodom” (36/100) i
„depopulacija pilića” (51/100), a za internu potkategoriju „čišćenje i dezinfekcija”
(37/100). Sistem za procenu implentiranih biosigurnosnih mera može se posmatrati kao vredan instrument za praćenje nivoa biosigurnosti. Periodična procena
biosigurnosti na brojlerskim farmama na nivou cele zemlje, omogućila bi mapiranje farmi prema nivou biosigurnosti i prema riziku od izbijanja određene bolesti.
AB  - Biosecurity implies the application of certain preventive measures on the farm
in order to reduce the possibility of the introduction and spread of pathogenic
microorganisms. Biosecurity can be external and internal. Biosecurity assessment
is a qualitative or quantitative determination of the level of biosecurity on farms.
The study was conducted on two broiler farms where the biosecurity assessment
was carried out using an online questionnaire – Biocheck.Ugent. The overall biosecurity score on farm 1 was 22% higher than that for farm 2. The external biosecurity score on farm 1 was 27% higher than that for farm 2, while the internal
biosecurity score differed by 8%. The external biosecurity score on farm 2 was
lower than the internal biosecurity score. Also, it was determined that the external
biosecurity, as well as the overall biosecurity implemented on farm 2, had a lower
score compared to the world average score obtained from Biochek.UGent database. Scores obtained for subcategories differed between farms. The lowest scores
for external biosecurity were for the subcategories ”manure and carcass removal” (5/100), ”food and water supply” (36/100), and ”depopulation” (51/100), and
for the internal subcategory ”cleaning and disinfection” (37/100). The system for
evaluating of implemented biosecurity measures can be seen as a valuable instrument for monitoring the level of biosecurity. A periodic assessment of biosecurity
on broiler farms at the country level would enable the mapping of farms according to the level of biosecurity and according to the risk of the outbreak of a certain
disease.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za DDD
PB  - Beograd : Fakultet veterinarske medicine, Katedra za zoohigijenu
C3  - 34. Savetovanje Dezinfekcija, dezinsekcija i deratizacija : Jedan svet  - jedno zdravlje
T1  - Značaj procene nivoa biosigurnosti na brojlerskim farmama
T1  - The importance of biosecurity assessment on the broiler farms
EP  - 109
SP  - 102
ER  - 

@conference{
author = "Maletić, Jelena and Kureljušić, Jasna and Milovanović, Bojan and Milićević, Vesna and Radosavljevic, Vladimir and Spalević, Ljiljana and Kureljušić, Branislav",
year = "2023",
abstract = "Biosigurnost podrazumeva primenu određenih preventivnih mera na farmi u
cilju smanjenja mogućnosti unošenja i širenja patogenih mikroorganizama. Biosigurnost može biti spoljašnja (eksterna) i unutrašnja (interna). Procena biosigurnosti predstavlja kvalitativno ili kvantitativno određivanje nivoa biosigurnosti na
farmama. Istraživanje je sprovedeno na dve brojlerske farme na kojima je procena nivoa biosigurnosti sprovedena upotrebom on-lajn upitnika – Biocheck.Ugent.
Ukupna ocena biosigurnosti na farmi 1 bila je viša za 22% u odnosu na ocenu za
farmu 2. Ocena eksterne biosigurnosti na farmi 1 je za 27% viša u odnosu na ocenu za farmu 2, dok su se ocene za internu biosigurnost razlikovale za 8%. Takođe,
utvrđno je da su ocena eksterne biosigurnosti, kao i ukupna ocena za biosigurnosne mere koje se sprovode na farmi 2, niže u odnosu na svetski prosek dobijen iz
baze podataka Biochek.UGent-a. Dobijene ocene za potkategorije, razlikovale su
se između farmi. Najniže ocene za eksternu biosigurnost dobile su potkategorije
„uklanjanje stajnjaka i leševa” (5/100), „snabdevanje hranom i vodom” (36/100) i
„depopulacija pilića” (51/100), a za internu potkategoriju „čišćenje i dezinfekcija”
(37/100). Sistem za procenu implentiranih biosigurnosnih mera može se posmatrati kao vredan instrument za praćenje nivoa biosigurnosti. Periodična procena
biosigurnosti na brojlerskim farmama na nivou cele zemlje, omogućila bi mapiranje farmi prema nivou biosigurnosti i prema riziku od izbijanja određene bolesti., Biosecurity implies the application of certain preventive measures on the farm
in order to reduce the possibility of the introduction and spread of pathogenic
microorganisms. Biosecurity can be external and internal. Biosecurity assessment
is a qualitative or quantitative determination of the level of biosecurity on farms.
The study was conducted on two broiler farms where the biosecurity assessment
was carried out using an online questionnaire – Biocheck.Ugent. The overall biosecurity score on farm 1 was 22% higher than that for farm 2. The external biosecurity score on farm 1 was 27% higher than that for farm 2, while the internal
biosecurity score differed by 8%. The external biosecurity score on farm 2 was
lower than the internal biosecurity score. Also, it was determined that the external
biosecurity, as well as the overall biosecurity implemented on farm 2, had a lower
score compared to the world average score obtained from Biochek.UGent database. Scores obtained for subcategories differed between farms. The lowest scores
for external biosecurity were for the subcategories ”manure and carcass removal” (5/100), ”food and water supply” (36/100), and ”depopulation” (51/100), and
for the internal subcategory ”cleaning and disinfection” (37/100). The system for
evaluating of implemented biosecurity measures can be seen as a valuable instrument for monitoring the level of biosecurity. A periodic assessment of biosecurity
on broiler farms at the country level would enable the mapping of farms according to the level of biosecurity and according to the risk of the outbreak of a certain
disease.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za DDD, Beograd : Fakultet veterinarske medicine, Katedra za zoohigijenu",
journal = "34. Savetovanje Dezinfekcija, dezinsekcija i deratizacija : Jedan svet  - jedno zdravlje",
title = "Značaj procene nivoa biosigurnosti na brojlerskim farmama, The importance of biosecurity assessment on the broiler farms",
pages = "109-102"
}

Maletić, J., Kureljušić, J., Milovanović, B., Milićević, V., Radosavljevic, V., Spalević, L.,& Kureljušić, B.. (2023). Značaj procene nivoa biosigurnosti na brojlerskim farmama. in 34. Savetovanje Dezinfekcija, dezinsekcija i deratizacija : Jedan svet  - jedno zdravlje
Beograd : Srpsko veterinarsko društvo, Sekcija za DDD., 102-109.

Maletić J, Kureljušić J, Milovanović B, Milićević V, Radosavljevic V, Spalević L, Kureljušić B. Značaj procene nivoa biosigurnosti na brojlerskim farmama. in 34. Savetovanje Dezinfekcija, dezinsekcija i deratizacija : Jedan svet  - jedno zdravlje. 2023;:102-109..

Maletić, Jelena, Kureljušić, Jasna, Milovanović, Bojan, Milićević, Vesna, Radosavljevic, Vladimir, Spalević, Ljiljana, Kureljušić, Branislav, "Značaj procene nivoa biosigurnosti na brojlerskim farmama" in 34. Savetovanje Dezinfekcija, dezinsekcija i deratizacija : Jedan svet  - jedno zdravlje (2023):102-109.

TY  - CONF
AU  - Jezdimirović, Nemanja
AU  - Kureljušić, Branislav
AU  - Savić, Božidar
AU  - Milovanović, Bojan
AU  - Glišić, Dimitrije
AU  - Maksimović Zorić, Jelena
AU  - Milićević, Vesna
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/767
AB  - Kliničko oboljenje uzrokovano sa porcine citomegalovirus (PCMV) se javlja samo
kod svinja, iako se infekcije srodnim virusima pojavljuju i kod drugih životinjskih vrsta
uključujući i ljude. Citomegalovirus kod svinja uzrokuje rinitis sa inkluzionim
telašcima (IBR), PCMV pripada podfamiliji Betaherpesvirinae i familiji Herpesviridae,
ali nije svrstan ni u jedan rod, a genetska istraživanja su pokazala da je PCMV genetski
srodniji humanim herpes virusima 6 i 7. Infekcija sa PCMV je prisutna širom sveta,
dominantno u zemljama sa intenzivnom proizvodnjom svinja i uglavnom je klinički
inaparentna zbog stečenog imuniteta zapata. Virus se prenosi horizontalno
(oronazalnim putem), a moguća je i kongenitalna transmisija, pa inficirani fetusi mogu
biti, mumificirani, mrtvorođeni ili rođeni živi kao slabo vitalna prasad. Kliničko
oboljenje se pojavljuje u akutnoj ili subakutnoj formi kod prasadi starosti do 4 nedelje,
a može se pojaviti i kod drugih starosnih kategorija u neimunim zapatima. Svinje koje
su prebolele infekciju ostaju doživotne kliconoše. Suide predstavljaju jedini rezervoar
virusa u prirodi. Rinitis sa inkluzionim telašcima je visoko prevalentna infekcija i
pretpostavlja se da je prevalencija oboljenja preko 90% na globalnom nivou, odnosno
98% u Evropi. U našoj zemlji još uvek nisu rađena opsežna istraživanja na utvrđivanju
prevalence oboljenja zbog čega su prevalenca infekcije i incidenca oboljenja još uvek
nepoznate. Zbog cirkulacije drugih respiratornih virusa u farmskim uslovima uzgoja
svinja, PCMV je često zapostavljen. Na poziv veterinarske službe, na farmi koja je imala učestale probleme sa
respiratornim oboljenjima, izvršili smo uzorkovanje oralnog fluida i analizirali
prikupljene uzorke primenom lančane reakcije polimeraze na prisustvo sledećih
virusnih agenasa: Encephalomyocarditis virus (EMCV), PCMV, Porcine circovirus tip 2
(PCV2). Virus reproduktivnog i respiratornog sindroma svinja (PRRSV) i Virus
influence svinja (SIV), sa ciljem uvida u prisustvo virusnih infektivnih agenasa na
farmi. Izvršenim analizama smo pored detekcije PRRSV, PCV2 i SIV, ustanovili i
prisustvo genoma PCMV kod prasadi iz odgoja. Mortalitet prasadi u neimunim
zapatima od posledica kliničkog PCMV-rinitisa može dostići 10%, ali se može povećati
do čak 50% ukoliko se bolest komplikuje drugim virusnim ili bakterijskim infekcijama.
Klinički značaj prisustva PCMV u konkretnom slučaju bi trebalo razmatrati u
kontekstu detekcije drugih virusnih patogena i njihovog sinergističkog efekta.
Međutim, obzirom da PCMV samostalno ili u koinfekcijama sa drugim, pre svega
virusnim respiratornim agensima može biti uzrok povećanja mortaliteta, ne treba ga
zanemariti. Vakcina protiv PCMV i specifična terapija ovog oboljenja ne postoje, sa
druge strane, ne postojanje informacija o prevalenci PCMV u našoj zemlji kao ni
vršenje rutinskog pregleda na ovu infekciju, predstavlja značajan rizik za širenje
virusa pre svega uvođenjem novonabavljenih životinja u zapat.
PB  - Beograd : Srpsko veterinarsko društvo
C3  - 34. Savetovanje veterinara Srbije, Zlatibor,  7-10. septembar 2023
T1  - Prva molekularna detekcija citomegalovirusa svinja u Srbiji
EP  - 95
SP  - 90
ER  - 

@conference{
author = "Jezdimirović, Nemanja and Kureljušić, Branislav and Savić, Božidar and Milovanović, Bojan and Glišić, Dimitrije and Maksimović Zorić, Jelena and Milićević, Vesna",
year = "2023",
abstract = "Kliničko oboljenje uzrokovano sa porcine citomegalovirus (PCMV) se javlja samo
kod svinja, iako se infekcije srodnim virusima pojavljuju i kod drugih životinjskih vrsta
uključujući i ljude. Citomegalovirus kod svinja uzrokuje rinitis sa inkluzionim
telašcima (IBR), PCMV pripada podfamiliji Betaherpesvirinae i familiji Herpesviridae,
ali nije svrstan ni u jedan rod, a genetska istraživanja su pokazala da je PCMV genetski
srodniji humanim herpes virusima 6 i 7. Infekcija sa PCMV je prisutna širom sveta,
dominantno u zemljama sa intenzivnom proizvodnjom svinja i uglavnom je klinički
inaparentna zbog stečenog imuniteta zapata. Virus se prenosi horizontalno
(oronazalnim putem), a moguća je i kongenitalna transmisija, pa inficirani fetusi mogu
biti, mumificirani, mrtvorođeni ili rođeni živi kao slabo vitalna prasad. Kliničko
oboljenje se pojavljuje u akutnoj ili subakutnoj formi kod prasadi starosti do 4 nedelje,
a može se pojaviti i kod drugih starosnih kategorija u neimunim zapatima. Svinje koje
su prebolele infekciju ostaju doživotne kliconoše. Suide predstavljaju jedini rezervoar
virusa u prirodi. Rinitis sa inkluzionim telašcima je visoko prevalentna infekcija i
pretpostavlja se da je prevalencija oboljenja preko 90% na globalnom nivou, odnosno
98% u Evropi. U našoj zemlji još uvek nisu rađena opsežna istraživanja na utvrđivanju
prevalence oboljenja zbog čega su prevalenca infekcije i incidenca oboljenja još uvek
nepoznate. Zbog cirkulacije drugih respiratornih virusa u farmskim uslovima uzgoja
svinja, PCMV je često zapostavljen. Na poziv veterinarske službe, na farmi koja je imala učestale probleme sa
respiratornim oboljenjima, izvršili smo uzorkovanje oralnog fluida i analizirali
prikupljene uzorke primenom lančane reakcije polimeraze na prisustvo sledećih
virusnih agenasa: Encephalomyocarditis virus (EMCV), PCMV, Porcine circovirus tip 2
(PCV2). Virus reproduktivnog i respiratornog sindroma svinja (PRRSV) i Virus
influence svinja (SIV), sa ciljem uvida u prisustvo virusnih infektivnih agenasa na
farmi. Izvršenim analizama smo pored detekcije PRRSV, PCV2 i SIV, ustanovili i
prisustvo genoma PCMV kod prasadi iz odgoja. Mortalitet prasadi u neimunim
zapatima od posledica kliničkog PCMV-rinitisa može dostići 10%, ali se može povećati
do čak 50% ukoliko se bolest komplikuje drugim virusnim ili bakterijskim infekcijama.
Klinički značaj prisustva PCMV u konkretnom slučaju bi trebalo razmatrati u
kontekstu detekcije drugih virusnih patogena i njihovog sinergističkog efekta.
Međutim, obzirom da PCMV samostalno ili u koinfekcijama sa drugim, pre svega
virusnim respiratornim agensima može biti uzrok povećanja mortaliteta, ne treba ga
zanemariti. Vakcina protiv PCMV i specifična terapija ovog oboljenja ne postoje, sa
druge strane, ne postojanje informacija o prevalenci PCMV u našoj zemlji kao ni
vršenje rutinskog pregleda na ovu infekciju, predstavlja značajan rizik za širenje
virusa pre svega uvođenjem novonabavljenih životinja u zapat.",
publisher = "Beograd : Srpsko veterinarsko društvo",
journal = "34. Savetovanje veterinara Srbije, Zlatibor,  7-10. septembar 2023",
title = "Prva molekularna detekcija citomegalovirusa svinja u Srbiji",
pages = "95-90"
}

Jezdimirović, N., Kureljušić, B., Savić, B., Milovanović, B., Glišić, D., Maksimović Zorić, J.,& Milićević, V.. (2023). Prva molekularna detekcija citomegalovirusa svinja u Srbiji. in 34. Savetovanje veterinara Srbije, Zlatibor,  7-10. septembar 2023
Beograd : Srpsko veterinarsko društvo., 90-95.

Jezdimirović N, Kureljušić B, Savić B, Milovanović B, Glišić D, Maksimović Zorić J, Milićević V. Prva molekularna detekcija citomegalovirusa svinja u Srbiji. in 34. Savetovanje veterinara Srbije, Zlatibor,  7-10. septembar 2023. 2023;:90-95..

Jezdimirović, Nemanja, Kureljušić, Branislav, Savić, Božidar, Milovanović, Bojan, Glišić, Dimitrije, Maksimović Zorić, Jelena, Milićević, Vesna, "Prva molekularna detekcija citomegalovirusa svinja u Srbiji" in 34. Savetovanje veterinara Srbije, Zlatibor,  7-10. septembar 2023 (2023):90-95.

TY  - JOUR
AU  - Glišić, Dimitrije
AU  - Zurovac Sapundžić, Zorana
AU  - Ninković, Milan
AU  - Milovanović, Bojan
AU  - Stevanović, Oliver
AU  - Laušević, Dejan
AU  - Đorđević, Milutin
AU  - Kureljušić, Branislav
AU  - Milićević, Vesna
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/724
AB  - This study aimed to describe the clinical course of SA-MCF and the duration of 
viremia in surviving cattle and to perform the phylogenetic analysis of the tegument 
protein gene of OvHV-2 in cattle in the Central Balkan. A farm housing two heifers 
and 7 sheep with a confirmed SA-MCF case were selected for the investigation. For 
the estimation of the length of viremia and the virus shedding, the animals were 
sampled repeatedly, weekly for two months. For the phylogenetic analysis, a 
retrospective study was performed on 21 samples from cattle, and 7 samples from 
sheep, from the Central Balkan. In the blood samples of the survived heifer, the 
OvHV-2 genome was detected until week 7, in corneal swabs, the OvHV-2 genome 
was detected until week 6, and in nasal swabs until week two of the study. A 
retrospective study revealed that out of 21 tested cattle, OvHV–2 was detected in 15 
(71.4%), and out of 7 tested sheep, three (42.9%) were positive. The sequenced 
samples show the highest percentage of similarity with the strains from Brasil 
KJ658293.1 (100%) and Germany HM216475.1 (100%).. Since there is a variety of 
different clinical signs similar to other notifiable diseases such as BVD, IBR, and 
FMD, there is a clear benefit in including SA-MCF in the differential diagnosis in 
cattle. As mixed farming in the Central Balkan is practiced, implementing SA-MCF 
monitoring in passive surveillance would allow a better understanding of the 
disease, ascertaining its prevalence and could provide new information regarding 
SA-MCF epidemiology.
PB  - Faislabad : Faculty of Veterinary Science, University of Agriculture
T2  - Pakistan Veterinary Journal
T1  - Detection and phylogenetic analysis of the Tegument protein gene of malignant catarrhal fever virus from clinical cases of cattle and sheep in the Central Balkan region
EP  - 365
IS  - 2
SP  - 361
VL  - 43
DO  - 10.29261/pakvetj/2023.032
ER  - 

@article{
author = "Glišić, Dimitrije and Zurovac Sapundžić, Zorana and Ninković, Milan and Milovanović, Bojan and Stevanović, Oliver and Laušević, Dejan and Đorđević, Milutin and Kureljušić, Branislav and Milićević, Vesna",
year = "2023",
abstract = "This study aimed to describe the clinical course of SA-MCF and the duration of 
viremia in surviving cattle and to perform the phylogenetic analysis of the tegument 
protein gene of OvHV-2 in cattle in the Central Balkan. A farm housing two heifers 
and 7 sheep with a confirmed SA-MCF case were selected for the investigation. For 
the estimation of the length of viremia and the virus shedding, the animals were 
sampled repeatedly, weekly for two months. For the phylogenetic analysis, a 
retrospective study was performed on 21 samples from cattle, and 7 samples from 
sheep, from the Central Balkan. In the blood samples of the survived heifer, the 
OvHV-2 genome was detected until week 7, in corneal swabs, the OvHV-2 genome 
was detected until week 6, and in nasal swabs until week two of the study. A 
retrospective study revealed that out of 21 tested cattle, OvHV–2 was detected in 15 
(71.4%), and out of 7 tested sheep, three (42.9%) were positive. The sequenced 
samples show the highest percentage of similarity with the strains from Brasil 
KJ658293.1 (100%) and Germany HM216475.1 (100%).. Since there is a variety of 
different clinical signs similar to other notifiable diseases such as BVD, IBR, and 
FMD, there is a clear benefit in including SA-MCF in the differential diagnosis in 
cattle. As mixed farming in the Central Balkan is practiced, implementing SA-MCF 
monitoring in passive surveillance would allow a better understanding of the 
disease, ascertaining its prevalence and could provide new information regarding 
SA-MCF epidemiology.",
publisher = "Faislabad : Faculty of Veterinary Science, University of Agriculture",
journal = "Pakistan Veterinary Journal",
title = "Detection and phylogenetic analysis of the Tegument protein gene of malignant catarrhal fever virus from clinical cases of cattle and sheep in the Central Balkan region",
pages = "365-361",
number = "2",
volume = "43",
doi = "10.29261/pakvetj/2023.032"
}

Glišić, D., Zurovac Sapundžić, Z., Ninković, M., Milovanović, B., Stevanović, O., Laušević, D., Đorđević, M., Kureljušić, B.,& Milićević, V.. (2023). Detection and phylogenetic analysis of the Tegument protein gene of malignant catarrhal fever virus from clinical cases of cattle and sheep in the Central Balkan region. in Pakistan Veterinary Journal
Faislabad : Faculty of Veterinary Science, University of Agriculture., 43(2), 361-365.
https://doi.org/10.29261/pakvetj/2023.032

Glišić D, Zurovac Sapundžić Z, Ninković M, Milovanović B, Stevanović O, Laušević D, Đorđević M, Kureljušić B, Milićević V. Detection and phylogenetic analysis of the Tegument protein gene of malignant catarrhal fever virus from clinical cases of cattle and sheep in the Central Balkan region. in Pakistan Veterinary Journal. 2023;43(2):361-365.
doi:10.29261/pakvetj/2023.032 .

Glišić, Dimitrije, Zurovac Sapundžić, Zorana, Ninković, Milan, Milovanović, Bojan, Stevanović, Oliver, Laušević, Dejan, Đorđević, Milutin, Kureljušić, Branislav, Milićević, Vesna, "Detection and phylogenetic analysis of the Tegument protein gene of malignant catarrhal fever virus from clinical cases of cattle and sheep in the Central Balkan region" in Pakistan Veterinary Journal, 43, no. 2 (2023):361-365,
https://doi.org/10.29261/pakvetj/2023.032 . .

TY  - JOUR
AU  - Milovanović, Bojan
AU  - Kureljušić, Branislav
AU  - Milićević, Vesna
AU  - Zdravković, Nemanja
AU  - Kureljušić, Jasna
AU  - Marinković, Darko
AU  - Maletić, Milan
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/694
AB  - Mycoplasma spp. is the cause of serious cattle health disorders that lead to poor
reproductive efficiency. Chronic seminal vesiculitis, infertility, vulvovaginitis and
dystocia were observed in Mycoplasma spp. infection. The objectives of the present study
were to investigate the frequency of Mycoplasmas in the uterus of postpartum dairy
cows and its potential role in the occurrence of subclinical endometritis. Our study
included 102 Holstein Friesian dairy cows 22-32 days postpartum. Uterine samples for
cytological, bacteriological, and molecular analysis were provided using Cytoprint AI®.
Metricheck® was used for the assessment of cervicovaginal mucus. After detection
of Mycoplasma spp. genome in uterine samples, the swabs of the vulva, vagina, nasal
swab, and samples of milk were sampled in order to exclude possible contamination
with other microorganisms as potential trigger of subclinical endometritis. The genome
of Mycoplasma spp. in the uterus was confirmed in 4 cows (2.40%). Sequencing of
the 16S RNA revealed that detected mycoplasmas belonged to Mycoplasma bovigenitalium
and Mycoplasma tauri species, with a prevalence of 1.80% and 0.6%, respectively. The
cytological evaluation showed a 69.90%, 54.87% and 48.33% of polymorphonuclear cells
for M. bovigenitalium positive cows, and 61.64% for M. tauri positive cow. Bacteriological
examination revealed Trueperella pyogenes in uterine samples in all 4 observed cows. This
is the first study where M. tauri was detected in the uterus of a live cow with subclinical enodmetritis. In this paper we hypothesized that M. bovigenitalium and M. tauri may have
a certain role in the etiology of subclinical enodmetritis.
AB  - Mycoplasma spp. prouzrokuju oboljenja goveda koja za posledicu imaju smanjenje
reproduktivne efikasnosti muznih krava. Mycoplasma spp. kod goveda dovode do
hroničnog seminalnog vezikulitisa, vulvovaginitisa, distokije i neplodnosti. Cilj ove
studije je bio da se ispita učestalost mikoplazmi u materici kod krava nakon teljenja i
utvrdi da li postoji potencijalna veza sa nastankom subkliničkog endometritisa. Studija
je obuhvatila 102 muzne krave Holštajn Frizijske rase od 22-32. dana nakon teljenja.
Brisevi endometrijuma za citološku, bakteriološku i molekularnu analizu su dobijeni
korišćenjem Cytoprint AI®. Metricheck® je korišćen za procenu kvaliteta cervikovaginalne
sluzi. Nakon detekcije genoma Mycoplasma spp. u brisevima endometrijuma,
uzorkovani su brisevi vulve, vagine, nosa i uzorci mleka kako bi se isključila moguća
kontaminacija drugim mikroorganizmima kao potencijalnim prouzrokovačima
subkliničkog endometritisa. Genom Mycoplasma spp. u brisu endometrijuma je utvrđen
kod 4 krave (2,40%). Sekvenciranjem 16S RNK utvrđeno je da detektovane mikoplazme
pripadaju Mycoplasma bovigenitalium i Mycoplasma tauri vrstama, sa prevalencijom
od 1,80%, odnosno 0,6%. Citološkom analizom je utvrđen procenat polimorfonuklearnih
ćelija od 69,90%, 54,87% i 48,33% kod krava pozitivnih na M. bovigenitalium i
61,64% kod krave pozitivne na M. tauri. Bakteriološkom analizom je utvrđena Trueperella
pyogenes u uzorcima endometrijuma kod sve 4 ispitane krave. Ovo je prva studija u
kojoj je M. tauri otkrivena u brisu iz materice žive krave sa subkliničkim endometritisom.
U ovom radu, pretpostavljamo da M. bovigenitalium i M. tauri mogu imati izvesnu
ulogu u etiologiji subkliničkog endometritisa.
PB  - Beograd : Fakultet veterinarske medicine
T2  - Acta Veterinaria-Beograd
T1  - Detection of Mycoplasma bovigenitalium and Mycoplasma tauri in holstein friesian dairy cows with subclinical endometritis
T1  - Detekcija genoma Mycoplasma bovigenitalium i Mycoplasma tauri kod mlečnih krava holštajnfrizijske rase sa supkliničkim endometritisom
EP  - 431
IS  - 3
SP  - 421
VL  - 73
DO  - 10.2478/acve-2023-0031
ER  - 

@article{
author = "Milovanović, Bojan and Kureljušić, Branislav and Milićević, Vesna and Zdravković, Nemanja and Kureljušić, Jasna and Marinković, Darko and Maletić, Milan",
year = "2023",
abstract = "Mycoplasma spp. is the cause of serious cattle health disorders that lead to poor
reproductive efficiency. Chronic seminal vesiculitis, infertility, vulvovaginitis and
dystocia were observed in Mycoplasma spp. infection. The objectives of the present study
were to investigate the frequency of Mycoplasmas in the uterus of postpartum dairy
cows and its potential role in the occurrence of subclinical endometritis. Our study
included 102 Holstein Friesian dairy cows 22-32 days postpartum. Uterine samples for
cytological, bacteriological, and molecular analysis were provided using Cytoprint AI®.
Metricheck® was used for the assessment of cervicovaginal mucus. After detection
of Mycoplasma spp. genome in uterine samples, the swabs of the vulva, vagina, nasal
swab, and samples of milk were sampled in order to exclude possible contamination
with other microorganisms as potential trigger of subclinical endometritis. The genome
of Mycoplasma spp. in the uterus was confirmed in 4 cows (2.40%). Sequencing of
the 16S RNA revealed that detected mycoplasmas belonged to Mycoplasma bovigenitalium
and Mycoplasma tauri species, with a prevalence of 1.80% and 0.6%, respectively. The
cytological evaluation showed a 69.90%, 54.87% and 48.33% of polymorphonuclear cells
for M. bovigenitalium positive cows, and 61.64% for M. tauri positive cow. Bacteriological
examination revealed Trueperella pyogenes in uterine samples in all 4 observed cows. This
is the first study where M. tauri was detected in the uterus of a live cow with subclinical enodmetritis. In this paper we hypothesized that M. bovigenitalium and M. tauri may have
a certain role in the etiology of subclinical enodmetritis., Mycoplasma spp. prouzrokuju oboljenja goveda koja za posledicu imaju smanjenje
reproduktivne efikasnosti muznih krava. Mycoplasma spp. kod goveda dovode do
hroničnog seminalnog vezikulitisa, vulvovaginitisa, distokije i neplodnosti. Cilj ove
studije je bio da se ispita učestalost mikoplazmi u materici kod krava nakon teljenja i
utvrdi da li postoji potencijalna veza sa nastankom subkliničkog endometritisa. Studija
je obuhvatila 102 muzne krave Holštajn Frizijske rase od 22-32. dana nakon teljenja.
Brisevi endometrijuma za citološku, bakteriološku i molekularnu analizu su dobijeni
korišćenjem Cytoprint AI®. Metricheck® je korišćen za procenu kvaliteta cervikovaginalne
sluzi. Nakon detekcije genoma Mycoplasma spp. u brisevima endometrijuma,
uzorkovani su brisevi vulve, vagine, nosa i uzorci mleka kako bi se isključila moguća
kontaminacija drugim mikroorganizmima kao potencijalnim prouzrokovačima
subkliničkog endometritisa. Genom Mycoplasma spp. u brisu endometrijuma je utvrđen
kod 4 krave (2,40%). Sekvenciranjem 16S RNK utvrđeno je da detektovane mikoplazme
pripadaju Mycoplasma bovigenitalium i Mycoplasma tauri vrstama, sa prevalencijom
od 1,80%, odnosno 0,6%. Citološkom analizom je utvrđen procenat polimorfonuklearnih
ćelija od 69,90%, 54,87% i 48,33% kod krava pozitivnih na M. bovigenitalium i
61,64% kod krave pozitivne na M. tauri. Bakteriološkom analizom je utvrđena Trueperella
pyogenes u uzorcima endometrijuma kod sve 4 ispitane krave. Ovo je prva studija u
kojoj je M. tauri otkrivena u brisu iz materice žive krave sa subkliničkim endometritisom.
U ovom radu, pretpostavljamo da M. bovigenitalium i M. tauri mogu imati izvesnu
ulogu u etiologiji subkliničkog endometritisa.",
publisher = "Beograd : Fakultet veterinarske medicine",
journal = "Acta Veterinaria-Beograd",
title = "Detection of Mycoplasma bovigenitalium and Mycoplasma tauri in holstein friesian dairy cows with subclinical endometritis, Detekcija genoma Mycoplasma bovigenitalium i Mycoplasma tauri kod mlečnih krava holštajnfrizijske rase sa supkliničkim endometritisom",
pages = "431-421",
number = "3",
volume = "73",
doi = "10.2478/acve-2023-0031"
}

Milovanović, B., Kureljušić, B., Milićević, V., Zdravković, N., Kureljušić, J., Marinković, D.,& Maletić, M.. (2023). Detection of Mycoplasma bovigenitalium and Mycoplasma tauri in holstein friesian dairy cows with subclinical endometritis. in Acta Veterinaria-Beograd
Beograd : Fakultet veterinarske medicine., 73(3), 421-431.
https://doi.org/10.2478/acve-2023-0031

Milovanović B, Kureljušić B, Milićević V, Zdravković N, Kureljušić J, Marinković D, Maletić M. Detection of Mycoplasma bovigenitalium and Mycoplasma tauri in holstein friesian dairy cows with subclinical endometritis. in Acta Veterinaria-Beograd. 2023;73(3):421-431.
doi:10.2478/acve-2023-0031 .

Milovanović, Bojan, Kureljušić, Branislav, Milićević, Vesna, Zdravković, Nemanja, Kureljušić, Jasna, Marinković, Darko, Maletić, Milan, "Detection of Mycoplasma bovigenitalium and Mycoplasma tauri in holstein friesian dairy cows with subclinical endometritis" in Acta Veterinaria-Beograd, 73, no. 3 (2023):421-431,
https://doi.org/10.2478/acve-2023-0031 . .