TY  - CONF
AU  - Jezdimirović, Nemanja
AU  - Kureljušić, Branislav
AU  - Savić, Božidar
AU  - Milovanović, Bojan
AU  - Glišić, Dimitrije
AU  - Maletić, Jelena
AU  - Šolaja, Sofija
AU  - Milićević, Vesna
PY  - 2024
UR  - https://reponivs.nivs.rs/handle/123456789/952
AB  - Дивље свиње могу бити резервоари различитих бактерија, вируса и паразита и
могу се пренети на домаће свиње па и на људе индиректним или директним
контактом. Порцине цитомегаловирус (ПЦМВ) препознат је као главни
узрочник атрофичног ринитиса код свиња свих старосних категорија.
Међународни комитет за таксономију вируса (ИТЦВ) је порцине
цитомегаловирус сврстао у suid betaherpesvirus 2 (SuBHV2), и припада
подфамилији Betaherpesvirinae и фамилији Herpesviridae, али није сврстан ни у
један род. Генетска истраживања су показала да је ПЦМВ генетски сроднији
хуманим херпес вирусима (ХХВ) 6А, 6Б и 7. Поред тога, доказано је да ПЦМВ
доприноси репродуктивним проблемима код крмача и резултира раним
неонаталним губицима. Инфекција ПЦМВ-ом је раширена широм света и има
високу преваленцију, посебно у земљама са интензивном производњом свиња.
Клинички пролази непримећено због стеченог имунитета запата, и обично се
јавља код млађих категорија животиња. Нажалост, сама болест и инфекција су
углавном занемарени.
Како бисмо открили улогу дивљих свиња у епидемиологији ПЦМВ-а,
користили смо реал-тиме ПЦР тестирање 50 узорака (лимфни чвор, слезина и
бубрег) пореклом од одстрељених дивљих свиња.
Испитивање је потврдило присуство генома ПЦМВ у 4/50 (8%) узорака. Ови
резултати указују на релативно ниску али не и занемарљиву преваленцију
вируса у популацији дивљих свиња, што може да представља значајан ризик и
за популацију домаћих свиња.
AB  - Wild boars can be reservoirs of various bacteria, viruses, and parasites and can be
transmitted to domestic pigs and even humans through indirect or direct contact.
Porcine cytomegalovirus (PCMV) is recognized as the main cause of atrophic rhinitis
in pigs of all age categories. The International Committee on Taxonomy of Viruses
(ICTV) has classified porcine cytomegalovirus into suid betaherpesvirus 2
(SuBHV2), belonging to the subfamily Betaherpesvirinae and family Herpesviridae,
but it is not classified into any genus. Genetic research has shown that PCMV is
genetically closer to human herpes viruses (HHV) 6A, 6B, and 7. Additionally, it has
been proven that PCMV contributes to reproductive problems in sows and results in
early neonatal losses. PCMV infection is widespread worldwide and has a high
prevalence, especially in countries with intensive pig production. Clinically, it often
goes unnoticed due to acquired maternal immunity and usually occurs in younger
animal categories. Unfortunately, the disease itself and the infection are largely
neglected.
To discover the role of wild boars in PCMV epidemiology, we used real-time PCR
testing of 50 samples (lymph nodes, spleen, and kidney) originating from shot wild
boars.
Testing confirmed the presence of PCMV genome in 4 out of 50 (8%) samples. These
results indicate a relatively low but not negligible prevalence of the virus in the wild
boar population, which may pose a significant risk to the domestic pig population.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
T1  - Prva molekularna detekcija porcine citomegalovirusa kod divljih svinja u Srbiji
T1  - First molecular detection of porcine cytomegalovirus in wild boars in Serbia
EP  - 79
SP  - 78
ER  - 

@conference{
author = "Jezdimirović, Nemanja and Kureljušić, Branislav and Savić, Božidar and Milovanović, Bojan and Glišić, Dimitrije and Maletić, Jelena and Šolaja, Sofija and Milićević, Vesna",
year = "2024",
abstract = "Дивље свиње могу бити резервоари различитих бактерија, вируса и паразита и
могу се пренети на домаће свиње па и на људе индиректним или директним
контактом. Порцине цитомегаловирус (ПЦМВ) препознат је као главни
узрочник атрофичног ринитиса код свиња свих старосних категорија.
Међународни комитет за таксономију вируса (ИТЦВ) је порцине
цитомегаловирус сврстао у suid betaherpesvirus 2 (SuBHV2), и припада
подфамилији Betaherpesvirinae и фамилији Herpesviridae, али није сврстан ни у
један род. Генетска истраживања су показала да је ПЦМВ генетски сроднији
хуманим херпес вирусима (ХХВ) 6А, 6Б и 7. Поред тога, доказано је да ПЦМВ
доприноси репродуктивним проблемима код крмача и резултира раним
неонаталним губицима. Инфекција ПЦМВ-ом је раширена широм света и има
високу преваленцију, посебно у земљама са интензивном производњом свиња.
Клинички пролази непримећено због стеченог имунитета запата, и обично се
јавља код млађих категорија животиња. Нажалост, сама болест и инфекција су
углавном занемарени.
Како бисмо открили улогу дивљих свиња у епидемиологији ПЦМВ-а,
користили смо реал-тиме ПЦР тестирање 50 узорака (лимфни чвор, слезина и
бубрег) пореклом од одстрељених дивљих свиња.
Испитивање је потврдило присуство генома ПЦМВ у 4/50 (8%) узорака. Ови
резултати указују на релативно ниску али не и занемарљиву преваленцију
вируса у популацији дивљих свиња, што може да представља значајан ризик и
за популацију домаћих свиња., Wild boars can be reservoirs of various bacteria, viruses, and parasites and can be
transmitted to domestic pigs and even humans through indirect or direct contact.
Porcine cytomegalovirus (PCMV) is recognized as the main cause of atrophic rhinitis
in pigs of all age categories. The International Committee on Taxonomy of Viruses
(ICTV) has classified porcine cytomegalovirus into suid betaherpesvirus 2
(SuBHV2), belonging to the subfamily Betaherpesvirinae and family Herpesviridae,
but it is not classified into any genus. Genetic research has shown that PCMV is
genetically closer to human herpes viruses (HHV) 6A, 6B, and 7. Additionally, it has
been proven that PCMV contributes to reproductive problems in sows and results in
early neonatal losses. PCMV infection is widespread worldwide and has a high
prevalence, especially in countries with intensive pig production. Clinically, it often
goes unnoticed due to acquired maternal immunity and usually occurs in younger
animal categories. Unfortunately, the disease itself and the infection are largely
neglected.
To discover the role of wild boars in PCMV epidemiology, we used real-time PCR
testing of 50 samples (lymph nodes, spleen, and kidney) originating from shot wild
boars.
Testing confirmed the presence of PCMV genome in 4 out of 50 (8%) samples. These
results indicate a relatively low but not negligible prevalence of the virus in the wild
boar population, which may pose a significant risk to the domestic pig population.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.",
title = "Prva molekularna detekcija porcine citomegalovirusa kod divljih svinja u Srbiji, First molecular detection of porcine cytomegalovirus in wild boars in Serbia",
pages = "79-78"
}

Jezdimirović, N., Kureljušić, B., Savić, B., Milovanović, B., Glišić, D., Maletić, J., Šolaja, S.,& Milićević, V.. (2024). Prva molekularna detekcija porcine citomegalovirusa kod divljih svinja u Srbiji. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 78-79.

Jezdimirović N, Kureljušić B, Savić B, Milovanović B, Glišić D, Maletić J, Šolaja S, Milićević V. Prva molekularna detekcija porcine citomegalovirusa kod divljih svinja u Srbiji. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.. 2024;:78-79..

Jezdimirović, Nemanja, Kureljušić, Branislav, Savić, Božidar, Milovanović, Bojan, Glišić, Dimitrije, Maletić, Jelena, Šolaja, Sofija, Milićević, Vesna, "Prva molekularna detekcija porcine citomegalovirusa kod divljih svinja u Srbiji" in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god. (2024):78-79.

TY  - CONF
AU  - Savić, Božidar
AU  - Milićević, Vesna
AU  - Kureljušić, Branislav
AU  - Jezdimirović, Nemanja
AU  - Milovanović, Bojan
AU  - Maksimović Zorić, Jelena
AU  - Stevnčević, Ognjen
PY  - 2024
UR  - https://reponivs.nivs.rs/handle/123456789/953
AB  - Репродуктивни и респираторни синдром свиња (енгл. Porcine Reproductive and Respiratory
Syndrome – PRRS), је контагиозна вирусна афекција свиња која тренутно представља
економски најзначајније инфективно обољење свиња, ендемично у многим земљама у
свету укључујучи и нашу земљу. PRRS је узрокован са вирусом репродуктивног и
респираторног синдрома свиња (енгл. Porcine Reproductive and Respiratory Syndrome Virus
– PRRSV), а с краја 90-их година прошлог века, када је обољење по први пут
дијагностиковано у нашој земљи, постоји релативно ограничен број епидемиолошких
студија овог обољења и молекуларних истраживања овог вируса који циркулишу у
популацији свиња. Описана су два генотипа PRRSV: тип-1 или Европски тип (Lelystad -
прототип) и тип-2 или Амерички тип (VR-2332 – прототип). Од недавно, PRRSV тип-1 и
тип-2 су класификовани као две засебне врсте унутар рода Betaarterivirus и то као
Betaarterivirus suid 1 (PRRSV1) и Betaarterivirus suid 2 (PRRSV2). У оквиру генотипа 1
(PRRSV1) се разликују 4 генетске линије (подтипа) (1, 2, 3 и 4) у које су сврстани изолати
вируса пореклом из различитих географских региона Европе. Унутар подтипова 1 и 3, се
диференцирају кластери 1А-1Г и 3А-3Г док подтип 2 чини јединствена група изолата, без
дефинисаних кластера. У нашој земљи, PRRS је био искључиво узрокован генотипом 1
(PRRSV1), међутим, 2022 године, детектован је и генотип 2 вируса (PRRSV2), на фарми
која је вршила увоз свиња из Европске уније. Анализом генома овог вируса је установљено
да је најсличнији тзв. вакциналним-сојевима (vaccine-like strain), односно да је вирус
највероватније пореклом из вакцине (МLV PRRSV2 вакцина) која је употребљена за
имунизацију свиња против PRRSV2 у земљи извозници. Циљ овог истраживањ је био да
се установи генетски диверзитет изолата PRRSV1 пореклом са фарми свиња из наше земље
у периоду од 2012 до 2022 године. У ту сврху, извршено је секвенцирање 36 ОРФ5 гена
изолата PRRSV1 коришћењем Сангер методе, које су потом поређене и анализиране
заједно са 106 секвенци ОРФ5 гена PRRSV1 из генетских линија 1, 2 и 3, које репрезентују
комплетан спектар генетског диверзитета ових подтипова (секвенце кластера 1А-1Г, 3А-
3Г и линије 2), применом различитих модула са предефинисаним параметарима у оквиру
софтверских пакета CLC – MainWorkbench и Mega X. Прикупљене секвнеце ОРФ5 гена су
депоноване у „NCBI“ бази биоинформатичких података. Филогенетском анализом је
установљено да су сви изолати PRRSV1 детектовани у периооду од 2012 до 2022 године у
нашој земљи груписани у два подтипа, односно подтип 1 и 3. У оквиру линије 1, се налази
7 изолата и то: 2 изолата у кластеру 1А, 2 у кластеру 1Б, 2 у кластеру 1Д и један изолат у
кластеру 1Ф. У оквиру линије 2, 29 секвенци је груписано у кластер 3Ц. Добијени
резултати представљају важне информације за будуће епидемиолошке анализе PRRSV1
инфекције укључујући утврђивање повезаности односно сродности између изолата вируса,
поређење нових секвенци са секвенцама пореклом од вакциналних сојева вируса,
разликовање вакциналних од „дивљих“ сојева вируса и утврђивање „уноса“ нових вируса
у популацију. Оваква епидемиолошка истраживања треба искористити за установљавање
критичних тачака у биосигурносним мерама на конкретним фармама, те имплементирати
одговарајуће промене како би се PRRSV инфекција успешно превенирала и контролисала.
AB  - Porcine Reproductive and Respiratory Syndrome (PRRS) is a contagious viral disease
of swine that currently represents the most economically significant infectious disease
of pigs, endemic in many countries worldwide, including Serbia. PRRS is caused by the
Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). Since the late 1990s,
when the disease was first diagnosed in the Serbia, there have been only few
epidemiological and molecular studies on circulating PPRS-viruses. Two genotypes of
PRRSV have been described: type 1 or European type (prototype Lelystad) and type 2
or American type (prototype VR-2332). Recently, PRRSV type 1 and type 2 have been
classified as two separate species within the genus Betaarterivirus, namely
Betaarterivirus suid 1 (PRRSV1) and Betaarterivirus suid 2 (PRRSV2). Within
genotype 1 (PRRSV1), four genetic lineages (subtypes) (1, 2, 3, and 4) have been
distinguished, in which isolates from different geographic regions of Europe are
classified. Within subtypes 1 and 3, clusters 1A-1G and 3A-3G are further differentiated,
while subtype 2 consists of a unique group of isolates, without defined clusters. In Serbia,
PRRS was exclusively caused by genotype 1 (PRRSV1); however, in 2022, a genotype
2 (PRRSV2) was detected on a farm that imported pigs from the European Union.
Genome analysis of this virus revealed its similarity to vaccine-like strains, indicating
that the virus most likely originated from a vaccine (MLV PRRSV2 vaccine) used for
immunization against PRRSV2 in the exporting country.
The aim of this study was to establish the genetic diversity of PRRSV1 isolates
originating from pig farms in Serbia from 2012 to 2022. For this purpose, sequencing of
36 ORF5 gene of PRRSV1 was performed using the Sanger method, which were then
compared and analyzed together with 106 ORF5 gene sequences of PRRSV1 from
genetic lineages 1, 2, and 3, representing the full range of genetic diversity of this
subtype (sequences from 1A-1G, 3A-3G clusters, and lineage 2), by using different
modules with predefined parameters within the software packages CLC –
MainWorkbench and Mega X. The obtained ORF5 gene sequences were deposited in the
NCBI database. Phylogenetic analysis revealed that all PRRSV1 isolates detected in the
Serbia from 2012 to 2022 were grouped into two subtypes, subtype 1 and 3. Within
lineage 1, there were 7 isolates: 2 in cluster 1A, 2 in cluster 1B, 2 in cluster 1D, and one
isolate in cluster 1F. Within lineage 2, 29 sequences were grouped in cluster 3C.
The obtained results provide important information for future epidemiological studies of
PRRSV1 infection, including the relatedness between isolates, comparing new obtianed
sequences with sequences originating from vaccine strains, differentiating vaccine
strains from "wild" strains, and determining the introduction of new strains into the
population. Such epidemiological studies can be used for find critical points in the
biosecurity on the given farms and implement changes in the others to prevent and
control PRRSV infection.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
T1  - Genetski diverzitet ORF5 gena virusa reproduktivnog i respiratornog sindroma svinja tip 1 od 2012 do 2022 godine
T1  - Genetic diversity of the ORF5 gene of type 1 porcine reproductive and respiratory syndrome virus in Serbia from 2012 to 2022
EP  - 103
SP  - 102
ER  - 

@conference{
author = "Savić, Božidar and Milićević, Vesna and Kureljušić, Branislav and Jezdimirović, Nemanja and Milovanović, Bojan and Maksimović Zorić, Jelena and Stevnčević, Ognjen",
year = "2024",
abstract = "Репродуктивни и респираторни синдром свиња (енгл. Porcine Reproductive and Respiratory
Syndrome – PRRS), је контагиозна вирусна афекција свиња која тренутно представља
економски најзначајније инфективно обољење свиња, ендемично у многим земљама у
свету укључујучи и нашу земљу. PRRS је узрокован са вирусом репродуктивног и
респираторног синдрома свиња (енгл. Porcine Reproductive and Respiratory Syndrome Virus
– PRRSV), а с краја 90-их година прошлог века, када је обољење по први пут
дијагностиковано у нашој земљи, постоји релативно ограничен број епидемиолошких
студија овог обољења и молекуларних истраживања овог вируса који циркулишу у
популацији свиња. Описана су два генотипа PRRSV: тип-1 или Европски тип (Lelystad -
прототип) и тип-2 или Амерички тип (VR-2332 – прототип). Од недавно, PRRSV тип-1 и
тип-2 су класификовани као две засебне врсте унутар рода Betaarterivirus и то као
Betaarterivirus suid 1 (PRRSV1) и Betaarterivirus suid 2 (PRRSV2). У оквиру генотипа 1
(PRRSV1) се разликују 4 генетске линије (подтипа) (1, 2, 3 и 4) у које су сврстани изолати
вируса пореклом из различитих географских региона Европе. Унутар подтипова 1 и 3, се
диференцирају кластери 1А-1Г и 3А-3Г док подтип 2 чини јединствена група изолата, без
дефинисаних кластера. У нашој земљи, PRRS је био искључиво узрокован генотипом 1
(PRRSV1), међутим, 2022 године, детектован је и генотип 2 вируса (PRRSV2), на фарми
која је вршила увоз свиња из Европске уније. Анализом генома овог вируса је установљено
да је најсличнији тзв. вакциналним-сојевима (vaccine-like strain), односно да је вирус
највероватније пореклом из вакцине (МLV PRRSV2 вакцина) која је употребљена за
имунизацију свиња против PRRSV2 у земљи извозници. Циљ овог истраживањ је био да
се установи генетски диверзитет изолата PRRSV1 пореклом са фарми свиња из наше земље
у периоду од 2012 до 2022 године. У ту сврху, извршено је секвенцирање 36 ОРФ5 гена
изолата PRRSV1 коришћењем Сангер методе, које су потом поређене и анализиране
заједно са 106 секвенци ОРФ5 гена PRRSV1 из генетских линија 1, 2 и 3, које репрезентују
комплетан спектар генетског диверзитета ових подтипова (секвенце кластера 1А-1Г, 3А-
3Г и линије 2), применом различитих модула са предефинисаним параметарима у оквиру
софтверских пакета CLC – MainWorkbench и Mega X. Прикупљене секвнеце ОРФ5 гена су
депоноване у „NCBI“ бази биоинформатичких података. Филогенетском анализом је
установљено да су сви изолати PRRSV1 детектовани у периооду од 2012 до 2022 године у
нашој земљи груписани у два подтипа, односно подтип 1 и 3. У оквиру линије 1, се налази
7 изолата и то: 2 изолата у кластеру 1А, 2 у кластеру 1Б, 2 у кластеру 1Д и један изолат у
кластеру 1Ф. У оквиру линије 2, 29 секвенци је груписано у кластер 3Ц. Добијени
резултати представљају важне информације за будуће епидемиолошке анализе PRRSV1
инфекције укључујући утврђивање повезаности односно сродности између изолата вируса,
поређење нових секвенци са секвенцама пореклом од вакциналних сојева вируса,
разликовање вакциналних од „дивљих“ сојева вируса и утврђивање „уноса“ нових вируса
у популацију. Оваква епидемиолошка истраживања треба искористити за установљавање
критичних тачака у биосигурносним мерама на конкретним фармама, те имплементирати
одговарајуће промене како би се PRRSV инфекција успешно превенирала и контролисала., Porcine Reproductive and Respiratory Syndrome (PRRS) is a contagious viral disease
of swine that currently represents the most economically significant infectious disease
of pigs, endemic in many countries worldwide, including Serbia. PRRS is caused by the
Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). Since the late 1990s,
when the disease was first diagnosed in the Serbia, there have been only few
epidemiological and molecular studies on circulating PPRS-viruses. Two genotypes of
PRRSV have been described: type 1 or European type (prototype Lelystad) and type 2
or American type (prototype VR-2332). Recently, PRRSV type 1 and type 2 have been
classified as two separate species within the genus Betaarterivirus, namely
Betaarterivirus suid 1 (PRRSV1) and Betaarterivirus suid 2 (PRRSV2). Within
genotype 1 (PRRSV1), four genetic lineages (subtypes) (1, 2, 3, and 4) have been
distinguished, in which isolates from different geographic regions of Europe are
classified. Within subtypes 1 and 3, clusters 1A-1G and 3A-3G are further differentiated,
while subtype 2 consists of a unique group of isolates, without defined clusters. In Serbia,
PRRS was exclusively caused by genotype 1 (PRRSV1); however, in 2022, a genotype
2 (PRRSV2) was detected on a farm that imported pigs from the European Union.
Genome analysis of this virus revealed its similarity to vaccine-like strains, indicating
that the virus most likely originated from a vaccine (MLV PRRSV2 vaccine) used for
immunization against PRRSV2 in the exporting country.
The aim of this study was to establish the genetic diversity of PRRSV1 isolates
originating from pig farms in Serbia from 2012 to 2022. For this purpose, sequencing of
36 ORF5 gene of PRRSV1 was performed using the Sanger method, which were then
compared and analyzed together with 106 ORF5 gene sequences of PRRSV1 from
genetic lineages 1, 2, and 3, representing the full range of genetic diversity of this
subtype (sequences from 1A-1G, 3A-3G clusters, and lineage 2), by using different
modules with predefined parameters within the software packages CLC –
MainWorkbench and Mega X. The obtained ORF5 gene sequences were deposited in the
NCBI database. Phylogenetic analysis revealed that all PRRSV1 isolates detected in the
Serbia from 2012 to 2022 were grouped into two subtypes, subtype 1 and 3. Within
lineage 1, there were 7 isolates: 2 in cluster 1A, 2 in cluster 1B, 2 in cluster 1D, and one
isolate in cluster 1F. Within lineage 2, 29 sequences were grouped in cluster 3C.
The obtained results provide important information for future epidemiological studies of
PRRSV1 infection, including the relatedness between isolates, comparing new obtianed
sequences with sequences originating from vaccine strains, differentiating vaccine
strains from "wild" strains, and determining the introduction of new strains into the
population. Such epidemiological studies can be used for find critical points in the
biosecurity on the given farms and implement changes in the others to prevent and
control PRRSV infection.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.",
title = "Genetski diverzitet ORF5 gena virusa reproduktivnog i respiratornog sindroma svinja tip 1 od 2012 do 2022 godine, Genetic diversity of the ORF5 gene of type 1 porcine reproductive and respiratory syndrome virus in Serbia from 2012 to 2022",
pages = "103-102"
}

Savić, B., Milićević, V., Kureljušić, B., Jezdimirović, N., Milovanović, B., Maksimović Zorić, J.,& Stevnčević, O.. (2024). Genetski diverzitet ORF5 gena virusa reproduktivnog i respiratornog sindroma svinja tip 1 od 2012 do 2022 godine. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 102-103.

Savić B, Milićević V, Kureljušić B, Jezdimirović N, Milovanović B, Maksimović Zorić J, Stevnčević O. Genetski diverzitet ORF5 gena virusa reproduktivnog i respiratornog sindroma svinja tip 1 od 2012 do 2022 godine. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.. 2024;:102-103..

Savić, Božidar, Milićević, Vesna, Kureljušić, Branislav, Jezdimirović, Nemanja, Milovanović, Bojan, Maksimović Zorić, Jelena, Stevnčević, Ognjen, "Genetski diverzitet ORF5 gena virusa reproduktivnog i respiratornog sindroma svinja tip 1 od 2012 do 2022 godine" in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god. (2024):102-103.

TY  - CONF
AU  - Kureljušić, Branislav
AU  - Vićić, Ivan
AU  - Bradić, Milijana
AU  - Maletić, Jelena
AU  - Jezdimirović, Nemanja
AU  - Savić, Božidar
AU  - Milovanović, Bojan
AU  - Karabasil, Neđeljko
PY  - 2024
UR  - https://reponivs.nivs.rs/handle/123456789/955
AB  - Интензивирањем бројлерске производње дошло је до значајног повећања
преваленције различитих патолошких стања чија основна етиологија почива у
самом технолошком процесу производње. Појава контактног дерматитиса на
плантарној кожи екстремитета („foot pad dermatitis“), затим на скочном зглобу
(„hock burns“) и на грудима („breast burns“) данас представља врло раширену
појаву у интензивној производњи, наносећи значајне економске губитке услед
смањеног прираста и одбацивања трупова на линији клања. Поред интензивирања
бројлерске производње значајан утицај на њихову појаву имају исхрана, услови и
начин држања (врста простирке) и генетска предиспозиција. Најзначајнији
фактор ризика за настанак лезија је влажна простирка посебно у комбинацији са
штетним дејством амонијака. Појава лезија има веома велики значај као
индикатор добробити имајући у виду да постоје претпоставке да су установљене
лезије врло болне и да често онемогућавају нормално кретање пилића.
Макроскопским прегледом лезија на плантарној површини уочавају се
инфламаторне лезије праћене некрозом на јастучићима табана и/или на прстима.
Лезије се ређе могу установити на тибиометатарзалним зглобовима. Код благог
облика постоји дисколорација на кожи, хиперкератоза, а у тежим случајевима
јавња се едем, ерозије и улцерације.
Хистопатолошки, благе лезије се карактеришу умереном хиперплазијом и/или
хиперкератозом епидермиса, конгестијом и едемом, уз присуство благог
инфламаторног инфилтрата у форми периваскулитиса и субепителијалне
инфилтрације мононуклеарним ћелијама и мањим бројем гранулоцита. Умерене
суперфицијелне лезије се карактеришу врло израженом хиперплазијом и
хиперкератозом епидермиса, егзоцитозом, појавом пустула и красти, конгестијом
и инфилтрацијом дермиса мононуклеарним и гранулоцитиним инфилтратом.
Тешке, дубоке лезије се карактеришу некрозом епидермиса који је замењен
некротичним гнојним садржајем, појавом гранулационог ткива, израженом
инфламаторном инфилтрацијом (периваскулитис и субепителна инфилтрација)
доминантно хетерофилним гранулоцитима и мононуклеарним ћелијама.
Имајући у виду да је појава контактног дерматитиса све учесталија појава како на
фармама бројлерских пилића тако и на кланици приликом прегледа трупова на
линија клања неопходно је имплементирати програм контроле овог патолошког
стања и на тај начин побољшати добробит и обезбедити производњу квалитетног
и безбедног пилећег меса.
AB  - The intensification of broiler production has led to a significant increase in the
prevalence of various pathological conditions, whose basic etiology lies in the
production process itself. The occurrence of contact dermatitis on the plantar skin of
extremities ("foot pad dermatitis"), then on the hock joint ("hock burns"), and on the
breasts ("breast burns") is today a very widespread phenomenon in intensive production,
causing significant economic losses due to reduced growth and carcass rejection on the
slaughter line. In addition to the intensification of broiler production, significant factors
influencing their occurrence are nutrition, housing conditions (type of bedding), and
genetic predisposition. The most significant risk factor for lesion formation is wet
bedding, especially in combination with the harmful effects of ammonia. The
occurrence of lesions is very important as an indicator of welfare, considering that there
are assumptions that established lesions are very painful and often prevent normal
chicken movement. Macroscopic examination of lesions on the plantar surface reveals
inflammatory lesions accompanied by necrosis on the foot pads and/or toes. Lesions are
less commonly found on the tibiotarsal joints. In mild forms, discoloration of the skin,
hyperkeratosis, and in severe cases, edema, erosions, and ulcerations occur.
Histopathologically, mild lesions are characterized by moderate epidermal hyperplasia
and/or hyperkeratosis, congestion and edema, with the presence of mild inflammatory
infiltrate in the form of perivasculitis and subepithelial infiltration of mononuclear cells
and a small number of granulocytes. Moderate superficial lesions are characterized by
very pronounced epidermal hyperplasia and hyperkeratosis, exocytosis, appearance of
pustules and crusts, congestion, and infiltration of the dermis with mononuclear and
granulocytic infiltrate. Severe, deep lesions are characterized by epidermal necrosis
replaced by necrotic purulent content, appearance of granulation tissue, pronounced
inflammatory infiltration (perivasculitis and subepithelial infiltration) predominantly
with heterophilic granulocytes and mononuclear cells. Considering that the occurrence
of contact dermatitis is becoming more frequent both on broiler farms and in
slaughterhouses during carcass examination on the slaughter line, it is necessary to
implement a control program for this pathological condition
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
T1  - Kontaktni pododermatitis kod brojlerskih pilića – patološki aspekt -
T1  - Contact pododermatitis in broiler chickens – pathological aspect –
EP  - 147
SP  - 146
ER  - 

@conference{
author = "Kureljušić, Branislav and Vićić, Ivan and Bradić, Milijana and Maletić, Jelena and Jezdimirović, Nemanja and Savić, Božidar and Milovanović, Bojan and Karabasil, Neđeljko",
year = "2024",
abstract = "Интензивирањем бројлерске производње дошло је до значајног повећања
преваленције различитих патолошких стања чија основна етиологија почива у
самом технолошком процесу производње. Појава контактног дерматитиса на
плантарној кожи екстремитета („foot pad dermatitis“), затим на скочном зглобу
(„hock burns“) и на грудима („breast burns“) данас представља врло раширену
појаву у интензивној производњи, наносећи значајне економске губитке услед
смањеног прираста и одбацивања трупова на линији клања. Поред интензивирања
бројлерске производње значајан утицај на њихову појаву имају исхрана, услови и
начин држања (врста простирке) и генетска предиспозиција. Најзначајнији
фактор ризика за настанак лезија је влажна простирка посебно у комбинацији са
штетним дејством амонијака. Појава лезија има веома велики значај као
индикатор добробити имајући у виду да постоје претпоставке да су установљене
лезије врло болне и да често онемогућавају нормално кретање пилића.
Макроскопским прегледом лезија на плантарној површини уочавају се
инфламаторне лезије праћене некрозом на јастучићима табана и/или на прстима.
Лезије се ређе могу установити на тибиометатарзалним зглобовима. Код благог
облика постоји дисколорација на кожи, хиперкератоза, а у тежим случајевима
јавња се едем, ерозије и улцерације.
Хистопатолошки, благе лезије се карактеришу умереном хиперплазијом и/или
хиперкератозом епидермиса, конгестијом и едемом, уз присуство благог
инфламаторног инфилтрата у форми периваскулитиса и субепителијалне
инфилтрације мононуклеарним ћелијама и мањим бројем гранулоцита. Умерене
суперфицијелне лезије се карактеришу врло израженом хиперплазијом и
хиперкератозом епидермиса, егзоцитозом, појавом пустула и красти, конгестијом
и инфилтрацијом дермиса мононуклеарним и гранулоцитиним инфилтратом.
Тешке, дубоке лезије се карактеришу некрозом епидермиса који је замењен
некротичним гнојним садржајем, појавом гранулационог ткива, израженом
инфламаторном инфилтрацијом (периваскулитис и субепителна инфилтрација)
доминантно хетерофилним гранулоцитима и мононуклеарним ћелијама.
Имајући у виду да је појава контактног дерматитиса све учесталија појава како на
фармама бројлерских пилића тако и на кланици приликом прегледа трупова на
линија клања неопходно је имплементирати програм контроле овог патолошког
стања и на тај начин побољшати добробит и обезбедити производњу квалитетног
и безбедног пилећег меса., The intensification of broiler production has led to a significant increase in the
prevalence of various pathological conditions, whose basic etiology lies in the
production process itself. The occurrence of contact dermatitis on the plantar skin of
extremities ("foot pad dermatitis"), then on the hock joint ("hock burns"), and on the
breasts ("breast burns") is today a very widespread phenomenon in intensive production,
causing significant economic losses due to reduced growth and carcass rejection on the
slaughter line. In addition to the intensification of broiler production, significant factors
influencing their occurrence are nutrition, housing conditions (type of bedding), and
genetic predisposition. The most significant risk factor for lesion formation is wet
bedding, especially in combination with the harmful effects of ammonia. The
occurrence of lesions is very important as an indicator of welfare, considering that there
are assumptions that established lesions are very painful and often prevent normal
chicken movement. Macroscopic examination of lesions on the plantar surface reveals
inflammatory lesions accompanied by necrosis on the foot pads and/or toes. Lesions are
less commonly found on the tibiotarsal joints. In mild forms, discoloration of the skin,
hyperkeratosis, and in severe cases, edema, erosions, and ulcerations occur.
Histopathologically, mild lesions are characterized by moderate epidermal hyperplasia
and/or hyperkeratosis, congestion and edema, with the presence of mild inflammatory
infiltrate in the form of perivasculitis and subepithelial infiltration of mononuclear cells
and a small number of granulocytes. Moderate superficial lesions are characterized by
very pronounced epidermal hyperplasia and hyperkeratosis, exocytosis, appearance of
pustules and crusts, congestion, and infiltration of the dermis with mononuclear and
granulocytic infiltrate. Severe, deep lesions are characterized by epidermal necrosis
replaced by necrotic purulent content, appearance of granulation tissue, pronounced
inflammatory infiltration (perivasculitis and subepithelial infiltration) predominantly
with heterophilic granulocytes and mononuclear cells. Considering that the occurrence
of contact dermatitis is becoming more frequent both on broiler farms and in
slaughterhouses during carcass examination on the slaughter line, it is necessary to
implement a control program for this pathological condition",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.",
title = "Kontaktni pododermatitis kod brojlerskih pilića – patološki aspekt -, Contact pododermatitis in broiler chickens – pathological aspect –",
pages = "147-146"
}

Kureljušić, B., Vićić, I., Bradić, M., Maletić, J., Jezdimirović, N., Savić, B., Milovanović, B.,& Karabasil, N.. (2024). Kontaktni pododermatitis kod brojlerskih pilića – patološki aspekt -. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 146-147.

Kureljušić B, Vićić I, Bradić M, Maletić J, Jezdimirović N, Savić B, Milovanović B, Karabasil N. Kontaktni pododermatitis kod brojlerskih pilića – patološki aspekt -. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.. 2024;:146-147..

Kureljušić, Branislav, Vićić, Ivan, Bradić, Milijana, Maletić, Jelena, Jezdimirović, Nemanja, Savić, Božidar, Milovanović, Bojan, Karabasil, Neđeljko, "Kontaktni pododermatitis kod brojlerskih pilića – patološki aspekt -" in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god. (2024):146-147.

TY  - CONF
AU  - Vasić, Ana
AU  - Kureljušić, Jasna
AU  - Rokvić, Nikola
AU  - Glišić, Dimitrije
AU  - Milovanović, Bojan
AU  - Vasilev, Saša
AU  - Kureljušić, Branislav
AU  - Paunović, Milan
PY  - 2024
UR  - https://reponivs.nivs.rs/handle/123456789/949
AB  - Трихинелоза је широм света присутна зооноза која се преноси конзумирањем
сировог или неправилно термички обрађеног меса или производа од меса
дивљих и домаћих животиња заражених ларвама нематода рода Trichinella.
Дивљи месоједи имају значајну епидемиолошку улогу у силватичном циклусу у
Европи, најчешће ширење Trichinella spp. међу животињама је путем исхране
кроз конзумацију меса одбаченог приликом лова и ингестије лешева који у
мишићном ткиву садрже ларве трихинела.
Од четири врсте Trichinella присутне у Европи (T. spiralis, T. britovi, T. nativa и
T. pseudospiralis), у Србији је присуство Т. spiralis и Т. britovi потврђено код
дивљих месоједа. Иако су статус и трендови популација дивљих карнивора у
Србији стабилни, енормна експанзија популације шакала (Canis aureus) током
последњих 40 година, донела је нове изазове у контроли Trichinella spp.
Циљ нашег истраживања је био да стекнемо увид у преваленцију Trichinella spp.
у популацији дивљих месоједа ( шакали (Canis aureus), лисице (Vulpas vulpes),
дивље мачке (Felis silvaticus) и јазавци (Meles meles)) који су изловљени на три
локалитета који припадају Срему, Граду Београду и Браничевском региону и да
идентификујемо врсте дивљих. месоједа које учествују у природном циклусу
преношења Trichinella spp. у Србији.
Укупно 122 узорка мускулатуре језика животиња одстрељених током 2022. и
2023. године испитано је појединачно коришћењем метода вештачке дигестије.
Тестирано је 67 узорака пореклом од шакала, 43 узорка од лисице, 10 узорака од
јазаваца и 2 од дивљих мачака. Резултати су показали да је 26 (21,14 %) било
позитивно на присуство ларви Trichinella spp. Међу позитивним узорцима је
највише било пореклом од шакала 18 (69,23 %), 7 (26,92 %) узорака пореклом од
лисице, 1 (3,85 %) дивљих мачака и 0 позитивних јазаваца.
Шакали, лисице и дивље мачке укључени су у циклус преноса Trichinella spp. на
основу наших резултата. Молекуларно одређивање врсте трихинеле је
неопходно како би се боље разумели начини преноса и како спречити ширење
дивљих на домаће животиње.
AB  - Trichinellosis is a worldwide zoonosis acquired by consuming raw or improperly
cooked meat or meat-derived products from wild and domestic animals infected with
larvae of nematode worms of the genus Trichinella. Wild carnivores have major
epidemiological role in the sylvatic cycle in Europe, mostly spreading Trichinella spp.
within animals through carnivorous activities and by their scavenger behaviour.
From four Trichinella species present in Europe (namely T. spiralis, T. britovi, T.
nativa and T. pseudospiralis), in Serbia the presence of T. spiralis and T. britovi is
confirmed in wild carnivores. Although the status and trends of the populations of
wild carnivores in Serbia are stable, enormous expansion of Golden jackal (Canis
aureus) population during last 40 years has brought new challenge in the control of
Trichinella spp.
The aim of our research was to access the prevalence of Trichinella spp. in population
of wild carnivores (Golden jackal (Canis aureus), fox (Vulpes vulpes), wild cat (Felis
silvestris) and badger (Meles meles) in three districts in Serbia (Braničevski, Sremski
and City of Belgrade) and to identify wild carnivore species taking part of the natural
transmission cycle of Trichinella spp. in Serbia.
A total of 122 tongue muscle tissue samples of animals hunted down during 2022 and
2023 were examined using artificial digestion method. We tested 67 samples from
jackal, 43 from foxes, 10 from badgers i 2 from wild cats. The results showed that 26
(21.14 %) were positive for the presence of Trichinella spp. larvae, among them 18
(69.23 %) jackals, 7 (26.92 %) foxes, one (3.85 %) wild cat, while none was positive
from badgers.
Jackals, foxes and wild cats are involved in Trichinella spp. transmission cycle.
Further Trichinella species typing is necessary to better understand modes of
transmission and how to prevent spreading from wild to domestic animals.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
T1  - Trihineloza kod divljih mesojeda i njihov značaj u održanju Trichinella spp.
T1  - Trichinelosis in wild carnivores and their significance in natural cycle of Trichinella spp.
EP  - 35
SP  - 34
ER  - 

@conference{
author = "Vasić, Ana and Kureljušić, Jasna and Rokvić, Nikola and Glišić, Dimitrije and Milovanović, Bojan and Vasilev, Saša and Kureljušić, Branislav and Paunović, Milan",
year = "2024",
abstract = "Трихинелоза је широм света присутна зооноза која се преноси конзумирањем
сировог или неправилно термички обрађеног меса или производа од меса
дивљих и домаћих животиња заражених ларвама нематода рода Trichinella.
Дивљи месоједи имају значајну епидемиолошку улогу у силватичном циклусу у
Европи, најчешће ширење Trichinella spp. међу животињама је путем исхране
кроз конзумацију меса одбаченог приликом лова и ингестије лешева који у
мишићном ткиву садрже ларве трихинела.
Од четири врсте Trichinella присутне у Европи (T. spiralis, T. britovi, T. nativa и
T. pseudospiralis), у Србији је присуство Т. spiralis и Т. britovi потврђено код
дивљих месоједа. Иако су статус и трендови популација дивљих карнивора у
Србији стабилни, енормна експанзија популације шакала (Canis aureus) током
последњих 40 година, донела је нове изазове у контроли Trichinella spp.
Циљ нашег истраживања је био да стекнемо увид у преваленцију Trichinella spp.
у популацији дивљих месоједа ( шакали (Canis aureus), лисице (Vulpas vulpes),
дивље мачке (Felis silvaticus) и јазавци (Meles meles)) који су изловљени на три
локалитета који припадају Срему, Граду Београду и Браничевском региону и да
идентификујемо врсте дивљих. месоједа које учествују у природном циклусу
преношења Trichinella spp. у Србији.
Укупно 122 узорка мускулатуре језика животиња одстрељених током 2022. и
2023. године испитано је појединачно коришћењем метода вештачке дигестије.
Тестирано је 67 узорака пореклом од шакала, 43 узорка од лисице, 10 узорака од
јазаваца и 2 од дивљих мачака. Резултати су показали да је 26 (21,14 %) било
позитивно на присуство ларви Trichinella spp. Међу позитивним узорцима је
највише било пореклом од шакала 18 (69,23 %), 7 (26,92 %) узорака пореклом од
лисице, 1 (3,85 %) дивљих мачака и 0 позитивних јазаваца.
Шакали, лисице и дивље мачке укључени су у циклус преноса Trichinella spp. на
основу наших резултата. Молекуларно одређивање врсте трихинеле је
неопходно како би се боље разумели начини преноса и како спречити ширење
дивљих на домаће животиње., Trichinellosis is a worldwide zoonosis acquired by consuming raw or improperly
cooked meat or meat-derived products from wild and domestic animals infected with
larvae of nematode worms of the genus Trichinella. Wild carnivores have major
epidemiological role in the sylvatic cycle in Europe, mostly spreading Trichinella spp.
within animals through carnivorous activities and by their scavenger behaviour.
From four Trichinella species present in Europe (namely T. spiralis, T. britovi, T.
nativa and T. pseudospiralis), in Serbia the presence of T. spiralis and T. britovi is
confirmed in wild carnivores. Although the status and trends of the populations of
wild carnivores in Serbia are stable, enormous expansion of Golden jackal (Canis
aureus) population during last 40 years has brought new challenge in the control of
Trichinella spp.
The aim of our research was to access the prevalence of Trichinella spp. in population
of wild carnivores (Golden jackal (Canis aureus), fox (Vulpes vulpes), wild cat (Felis
silvestris) and badger (Meles meles) in three districts in Serbia (Braničevski, Sremski
and City of Belgrade) and to identify wild carnivore species taking part of the natural
transmission cycle of Trichinella spp. in Serbia.
A total of 122 tongue muscle tissue samples of animals hunted down during 2022 and
2023 were examined using artificial digestion method. We tested 67 samples from
jackal, 43 from foxes, 10 from badgers i 2 from wild cats. The results showed that 26
(21.14 %) were positive for the presence of Trichinella spp. larvae, among them 18
(69.23 %) jackals, 7 (26.92 %) foxes, one (3.85 %) wild cat, while none was positive
from badgers.
Jackals, foxes and wild cats are involved in Trichinella spp. transmission cycle.
Further Trichinella species typing is necessary to better understand modes of
transmission and how to prevent spreading from wild to domestic animals.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.",
title = "Trihineloza kod divljih mesojeda i njihov značaj u održanju Trichinella spp., Trichinelosis in wild carnivores and their significance in natural cycle of Trichinella spp.",
pages = "35-34"
}

Vasić, A., Kureljušić, J., Rokvić, N., Glišić, D., Milovanović, B., Vasilev, S., Kureljušić, B.,& Paunović, M.. (2024). Trihineloza kod divljih mesojeda i njihov značaj u održanju Trichinella spp.. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 34-35.

Vasić A, Kureljušić J, Rokvić N, Glišić D, Milovanović B, Vasilev S, Kureljušić B, Paunović M. Trihineloza kod divljih mesojeda i njihov značaj u održanju Trichinella spp.. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.. 2024;:34-35..

Vasić, Ana, Kureljušić, Jasna, Rokvić, Nikola, Glišić, Dimitrije, Milovanović, Bojan, Vasilev, Saša, Kureljušić, Branislav, Paunović, Milan, "Trihineloza kod divljih mesojeda i njihov značaj u održanju Trichinella spp." in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god. (2024):34-35.

TY  - CONF
AU  - Šolaja, Sofija
AU  - Glišić, Dimitrije
AU  - Zurovac Sapundžić, Zorana
AU  - Milovanović, Bojan
AU  - Maletić, Jelena
AU  - Jezdimirović, Nemanja
AU  - Kureljušić, Branislav
AU  - Milićević, Vesna
PY  - 2024
UR  - https://reponivs.nivs.rs/handle/123456789/954
AB  - Инфективне болести представљају један од главних проблема у интензивном узгоју свиња, упркос технолошком напретку и развоју биосигурносних мера. Вирусна респираторна обољења која имају највећи економски и здравствени значај у свињарству су репродуктивни и респираторни синдром свиња, инфлуенца свиња и Аујецкијева болест. Сеоска газдинства екстензивног типа, и даље предcтављају најчешћи начин узгоја свиња у Србији. Циљ истраживања је био да се утврди серопреваленција респираторних вируса на сеоским газдинствима која представљају извор патогена за комерцијалне фарме. Крвни серуми су узорковани од 222 јединке са 69 газдинстава на којима се налазило до 10 свиња. За детекцију антитела су коришћени комерцијални ЕЛИСА тестови према упутству произвођача. Установљено је 38 (17,1%) серопозитивних јединки на вирус Аујецкијеве болести и 6 (2,7%) на вирус репродуктивног и респираторног синдрома. Антитела на вирус инфлуенце свиња нису установљена ни код једне јединкe. Два газдинства (2,9%) су била серопозитивна на репродуктивни и респираторни синдром са серопреваленцијом у запату од 100%, без присуства антитела на друге тестиране вирусе. Проценат серопозитивних запата на Аујецкијеву болест је био 10,1%, а серопреваленција у запатима је варирала од 33% до 100%. Серонегативних запата на све три болести је било 48 (69,5%). Ови резултати указују да међу свињама на сеоским газдинствима највише циркулише вирус Аујецкијеве болести. Главне катактеристике сеоских газдинстава су неадекватне биосигурносне мере, исхрана помијама и неконтролисан транспорт и контакт са другим врстама животиња. Стога, будући да је здравствени статус оваквих запата непознат, они се сматрају извором високог ризика за комерцијалне фарме. Власници сеоских газдинстава су често запослени на комерцијалним фармама, без обзира на забрану држања свиња, и представљају везу између сеоских газдинстава и комерцијалних фарми. Неопходно је спровести мониторниг здравственог стања, побољшање биосигурносних мера и едукацију власника како би се спречило ширење инфективних обољења са сеоских газдинстава на комерцијалне фарме.
AB  - Infectious diseases represent one of the main challenges in intensive pig farming, despite technological advancements and biosecurity improvement. Viral respiratory diseases with the greatest economic and health significance in pig farming include porcine reproductive and respiratory syndrome (PRRS), swine influenza (SI), and Aujeszky's disease (AD). Small backyard farms continue to be the most common method of pig farming in Serbia. This research aimed to assess the prevalence of respiratory viruses on backyard farms, which serve as a source of pathogens for commercial farms. Serum samples were collected from 222 individuals on 69 farms, each housing up to 10 pigs. Commercial ELISA tests were used for antibody detection according to the manufacturer's instructions. Out of the samples, 38 (17.1%) were seropositive for AD, and 6 (2.7%) for PRRS. No antibodies to the swine influenza virus were detected. Two farms (2.9%) were seropositive for PRRS with a herd-level seroprevalence of 100%, with no antibodies detected for other tested viruses. The percentage of seropositive farms for Aujeszky's disease was 10.1%, with herd-level seroprevalence ranging from 33% to 100%. There were 48 (69.5%) seronegative herds for all three diseases. These results indicate that Aujeszky's disease virus circulates the most among pigs on backyard farms. Main characteristics of backyard farms are low biosecurity, swill feeding, uncontrolled trade and contact with other animal species. Thus, since the health status of backyard farms is unknown, they are considered a high-risk infection source for commercial farms. Owners of backyard farms are often employed on commercial farms, despite regulations, serving as a link between backyard and commercial farms. Implementation of monitoring, improvement of biosecurity measures and education of owners are crucial steps for preventing the transmission of infectious diseases between backyard and commercial farms.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
T1  - Seroprevalencija virusnih respiratornih oboljenja svinja na seoskim gazdinstvima u Srbiji
T1  - Seroprevalence of viral respiratory diseases in swine backyard farms in Serbia
EP  - 107
SP  - 106
ER  - 

@conference{
author = "Šolaja, Sofija and Glišić, Dimitrije and Zurovac Sapundžić, Zorana and Milovanović, Bojan and Maletić, Jelena and Jezdimirović, Nemanja and Kureljušić, Branislav and Milićević, Vesna",
year = "2024",
abstract = "Инфективне болести представљају један од главних проблема у интензивном узгоју свиња, упркос технолошком напретку и развоју биосигурносних мера. Вирусна респираторна обољења која имају највећи економски и здравствени значај у свињарству су репродуктивни и респираторни синдром свиња, инфлуенца свиња и Аујецкијева болест. Сеоска газдинства екстензивног типа, и даље предcтављају најчешћи начин узгоја свиња у Србији. Циљ истраживања је био да се утврди серопреваленција респираторних вируса на сеоским газдинствима која представљају извор патогена за комерцијалне фарме. Крвни серуми су узорковани од 222 јединке са 69 газдинстава на којима се налазило до 10 свиња. За детекцију антитела су коришћени комерцијални ЕЛИСА тестови према упутству произвођача. Установљено је 38 (17,1%) серопозитивних јединки на вирус Аујецкијеве болести и 6 (2,7%) на вирус репродуктивног и респираторног синдрома. Антитела на вирус инфлуенце свиња нису установљена ни код једне јединкe. Два газдинства (2,9%) су била серопозитивна на репродуктивни и респираторни синдром са серопреваленцијом у запату од 100%, без присуства антитела на друге тестиране вирусе. Проценат серопозитивних запата на Аујецкијеву болест је био 10,1%, а серопреваленција у запатима је варирала од 33% до 100%. Серонегативних запата на све три болести је било 48 (69,5%). Ови резултати указују да међу свињама на сеоским газдинствима највише циркулише вирус Аујецкијеве болести. Главне катактеристике сеоских газдинстава су неадекватне биосигурносне мере, исхрана помијама и неконтролисан транспорт и контакт са другим врстама животиња. Стога, будући да је здравствени статус оваквих запата непознат, они се сматрају извором високог ризика за комерцијалне фарме. Власници сеоских газдинстава су често запослени на комерцијалним фармама, без обзира на забрану држања свиња, и представљају везу између сеоских газдинстава и комерцијалних фарми. Неопходно је спровести мониторниг здравственог стања, побољшање биосигурносних мера и едукацију власника како би се спречило ширење инфективних обољења са сеоских газдинстава на комерцијалне фарме., Infectious diseases represent one of the main challenges in intensive pig farming, despite technological advancements and biosecurity improvement. Viral respiratory diseases with the greatest economic and health significance in pig farming include porcine reproductive and respiratory syndrome (PRRS), swine influenza (SI), and Aujeszky's disease (AD). Small backyard farms continue to be the most common method of pig farming in Serbia. This research aimed to assess the prevalence of respiratory viruses on backyard farms, which serve as a source of pathogens for commercial farms. Serum samples were collected from 222 individuals on 69 farms, each housing up to 10 pigs. Commercial ELISA tests were used for antibody detection according to the manufacturer's instructions. Out of the samples, 38 (17.1%) were seropositive for AD, and 6 (2.7%) for PRRS. No antibodies to the swine influenza virus were detected. Two farms (2.9%) were seropositive for PRRS with a herd-level seroprevalence of 100%, with no antibodies detected for other tested viruses. The percentage of seropositive farms for Aujeszky's disease was 10.1%, with herd-level seroprevalence ranging from 33% to 100%. There were 48 (69.5%) seronegative herds for all three diseases. These results indicate that Aujeszky's disease virus circulates the most among pigs on backyard farms. Main characteristics of backyard farms are low biosecurity, swill feeding, uncontrolled trade and contact with other animal species. Thus, since the health status of backyard farms is unknown, they are considered a high-risk infection source for commercial farms. Owners of backyard farms are often employed on commercial farms, despite regulations, serving as a link between backyard and commercial farms. Implementation of monitoring, improvement of biosecurity measures and education of owners are crucial steps for preventing the transmission of infectious diseases between backyard and commercial farms.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.",
title = "Seroprevalencija virusnih respiratornih oboljenja svinja na seoskim gazdinstvima u Srbiji, Seroprevalence of viral respiratory diseases in swine backyard farms in Serbia",
pages = "107-106"
}

Šolaja, S., Glišić, D., Zurovac Sapundžić, Z., Milovanović, B., Maletić, J., Jezdimirović, N., Kureljušić, B.,& Milićević, V.. (2024). Seroprevalencija virusnih respiratornih oboljenja svinja na seoskim gazdinstvima u Srbiji. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 106-107.

Šolaja S, Glišić D, Zurovac Sapundžić Z, Milovanović B, Maletić J, Jezdimirović N, Kureljušić B, Milićević V. Seroprevalencija virusnih respiratornih oboljenja svinja na seoskim gazdinstvima u Srbiji. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.. 2024;:106-107..

Šolaja, Sofija, Glišić, Dimitrije, Zurovac Sapundžić, Zorana, Milovanović, Bojan, Maletić, Jelena, Jezdimirović, Nemanja, Kureljušić, Branislav, Milićević, Vesna, "Seroprevalencija virusnih respiratornih oboljenja svinja na seoskim gazdinstvima u Srbiji" in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god. (2024):106-107.

TY  - CONF
AU  - Milovanović, Bojan
AU  - Stanojević, Slobodan
AU  - Kureljušić, Branislav
AU  - Zurovac Sapundžić, Zorana
AU  - Milićević, Vesna
AU  - Zdravković, Nemanja
AU  - Jezdimirović, Nemanja
AU  - Maletić, Milan
AU  - Savić, Božidar
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/816
AB  - Pojava povremenih pobačaja ne predstavlja razlog za zabrinutost. Dva glavna
kriterijuma kada pojava pobačaja postaje problem u zapatu su: ako stopa
pobačaja pređe 3% na 100 steonih krava ili 5% na 100 jagnjenih ovaca, ili
ukoliko veći broj životinja pobaci u kratkom vremenskom intervalu. U bilo koja
dva navedena slučaja neophodno je obavestiti ordinirajućeg veterinara i nadležnog
veterinarskog inspektora. Pobačaji se mogu podeliti na infektivne i neinfektivne.
Pobačaje infektivne prirode uzrokuju različiti virusi, bakterije (Brucella
abortus, Brucella ovis, Chlamydia abortus), gljivice, protozoe (Neospora
caninum) i rikecije (Coxiella burnetii). Uzročnici neinfektivne prirode mogu
biti: otrovne biljke, metabolički i hormonalni poremećaji, nutritivni deficiti,
traume i kongenitalne anomalije ploda. Etiološka dijagnoza pobačaja postavlja
se u svega 30% slučajeva – kod 40 do 50% slučajeva nije moguće utvrditi
uzrok. Podaci o zastupljenosti infektivnih agenasa kao uzročnika pobačaja variraju
u zavisnosti od regiona, načina držanja i uzoraka koji se dostavljaju na
laboratorijsko ispitivanje. Pored direktnog uticaja na zdravlje i dobrobit životinja,
pobačaji dovode i do ekonomskih gubitaka. Pojedini infektivni uzročnici
imaju zoonotski potencijal i predstavljaju opasnost po javno zdravlje. Zbog toga
prevencija pobačaja predstavlja imperativ u procesu proizvodnje.
AB  - The occurrence of periodical abortions is not a reason for concern. Two main
criteria when the abortions represent a problem on farm are: abortion rate
exceeds 3% per 100 pregnant cows or 5% per 100 pregnant ewes; certain
number of animals abort in a short time interval (abortion storm). In many of
two mentioned cases, it is necessary to inform the ordinating veterinarian and
the regional veterinary inspector. Etiology of abortions can be divided into
infectious and noninfectious. Infectious causes of abortions are viruses, bacteria
(Brucella abortus, Brucella ovis, Chlamydia abortus), fungi, protozoan (Neospora
caninum), rickettsias (Coxiella burnetii). Noninfectious abortions can be
caused by: heat stress, poisonous plants, metabolic and hormonal disorders,
nutritional deficits, trauma and congenital anomalies of fetus. The etiological
diagnosis of abortions is determinated in 30% of cases. In 40 to 50% of cases,
depending on the available laboratory diagnostics, it is not possible to establish
the cause. Data about prevalence of infectious agents vary depending on the
region, management and the samples that were submitted to diagnostic laboratory.
A spite of direct influence on animal health and welfare, abortions lead
to economic losses. Some infectious agents have zoonotic potential and represent
a risk to public health. Therefore, abortion control represent imperative in
the production process.
PB  - Beograd : Fakultet veterinarske medicine
C3  - Četvrti regionalni simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 29. jun - 1. jul 2023
T1  - Infektivni pobačaji preživara – zdravstveni i ekonomski značaj
T1  - Infectious abortions in ruminants–health and economic impact
EP  - 215
SP  - 207
ER  - 

@conference{
author = "Milovanović, Bojan and Stanojević, Slobodan and Kureljušić, Branislav and Zurovac Sapundžić, Zorana and Milićević, Vesna and Zdravković, Nemanja and Jezdimirović, Nemanja and Maletić, Milan and Savić, Božidar",
year = "2023",
abstract = "Pojava povremenih pobačaja ne predstavlja razlog za zabrinutost. Dva glavna
kriterijuma kada pojava pobačaja postaje problem u zapatu su: ako stopa
pobačaja pređe 3% na 100 steonih krava ili 5% na 100 jagnjenih ovaca, ili
ukoliko veći broj životinja pobaci u kratkom vremenskom intervalu. U bilo koja
dva navedena slučaja neophodno je obavestiti ordinirajućeg veterinara i nadležnog
veterinarskog inspektora. Pobačaji se mogu podeliti na infektivne i neinfektivne.
Pobačaje infektivne prirode uzrokuju različiti virusi, bakterije (Brucella
abortus, Brucella ovis, Chlamydia abortus), gljivice, protozoe (Neospora
caninum) i rikecije (Coxiella burnetii). Uzročnici neinfektivne prirode mogu
biti: otrovne biljke, metabolički i hormonalni poremećaji, nutritivni deficiti,
traume i kongenitalne anomalije ploda. Etiološka dijagnoza pobačaja postavlja
se u svega 30% slučajeva – kod 40 do 50% slučajeva nije moguće utvrditi
uzrok. Podaci o zastupljenosti infektivnih agenasa kao uzročnika pobačaja variraju
u zavisnosti od regiona, načina držanja i uzoraka koji se dostavljaju na
laboratorijsko ispitivanje. Pored direktnog uticaja na zdravlje i dobrobit životinja,
pobačaji dovode i do ekonomskih gubitaka. Pojedini infektivni uzročnici
imaju zoonotski potencijal i predstavljaju opasnost po javno zdravlje. Zbog toga
prevencija pobačaja predstavlja imperativ u procesu proizvodnje., The occurrence of periodical abortions is not a reason for concern. Two main
criteria when the abortions represent a problem on farm are: abortion rate
exceeds 3% per 100 pregnant cows or 5% per 100 pregnant ewes; certain
number of animals abort in a short time interval (abortion storm). In many of
two mentioned cases, it is necessary to inform the ordinating veterinarian and
the regional veterinary inspector. Etiology of abortions can be divided into
infectious and noninfectious. Infectious causes of abortions are viruses, bacteria
(Brucella abortus, Brucella ovis, Chlamydia abortus), fungi, protozoan (Neospora
caninum), rickettsias (Coxiella burnetii). Noninfectious abortions can be
caused by: heat stress, poisonous plants, metabolic and hormonal disorders,
nutritional deficits, trauma and congenital anomalies of fetus. The etiological
diagnosis of abortions is determinated in 30% of cases. In 40 to 50% of cases,
depending on the available laboratory diagnostics, it is not possible to establish
the cause. Data about prevalence of infectious agents vary depending on the
region, management and the samples that were submitted to diagnostic laboratory.
A spite of direct influence on animal health and welfare, abortions lead
to economic losses. Some infectious agents have zoonotic potential and represent
a risk to public health. Therefore, abortion control represent imperative in
the production process.",
publisher = "Beograd : Fakultet veterinarske medicine",
journal = "Četvrti regionalni simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 29. jun - 1. jul 2023",
title = "Infektivni pobačaji preživara – zdravstveni i ekonomski značaj, Infectious abortions in ruminants–health and economic impact",
pages = "215-207"
}

Milovanović, B., Stanojević, S., Kureljušić, B., Zurovac Sapundžić, Z., Milićević, V., Zdravković, N., Jezdimirović, N., Maletić, M.,& Savić, B.. (2023). Infektivni pobačaji preživara – zdravstveni i ekonomski značaj. in Četvrti regionalni simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 29. jun - 1. jul 2023
Beograd : Fakultet veterinarske medicine., 207-215.

Milovanović B, Stanojević S, Kureljušić B, Zurovac Sapundžić Z, Milićević V, Zdravković N, Jezdimirović N, Maletić M, Savić B. Infektivni pobačaji preživara – zdravstveni i ekonomski značaj. in Četvrti regionalni simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 29. jun - 1. jul 2023. 2023;:207-215..

Milovanović, Bojan, Stanojević, Slobodan, Kureljušić, Branislav, Zurovac Sapundžić, Zorana, Milićević, Vesna, Zdravković, Nemanja, Jezdimirović, Nemanja, Maletić, Milan, Savić, Božidar, "Infektivni pobačaji preživara – zdravstveni i ekonomski značaj" in Četvrti regionalni simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 29. jun - 1. jul 2023 (2023):207-215.

TY  - JOUR
AU  - Glišić, Dimitrije
AU  - Veljović, Ljubiša
AU  - Milovanović, Bojan
AU  - Ninković, Milan
AU  - Maletić, Jelena
AU  - Kureljušić, Branislav
AU  - Milićević, Vesna
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/849
AB  - Monitoring the health of swine herds is essential to ensure good manufacturing
practices. Traditionally, active and passive surveillance on farms involved invasive
sampling methods, where specific animals were selected, restrained, and sampled.
However, with the increasing intensity of swine production, alternative methods for
effective herd surveillance became necessary.
Non-invasive sampling provides a convenient and cost-effective approach to monitor
the entire herd without compromising animal welfare, while still obtaining suitable
samples for testing. Oral fluids have been widely used in both human and livestock
health surveillance for various viral pathogens, including significant diseases. Nasal
wipes (NW) utilize different cloth materials soaked in phosphate-buffered saline
(PBS) or tissue culture medium with antibiotics and antimycotics to sample for swine
influenza virus (SIV). Udder skin wipes (USW) offer an alternative method to assess
the health status of piglets in a litter. During routine procedures such as tail docking
and castration, a mixture of blood and serum can be collected, known as process fluids
(PF), which has proven successful in monitoring herds for the presence of porcine
reproductive and respiratory syndrome virus (PRRSV). Furthermore, air sampling has emerged as a novel technique to detect pathogens in various farming systems and
animal species. This method offers the advantage of obtaining diagnostic samples
without direct animal contact.
By employing these non-invasive sampling methods, swine producers can implement
effective surveillance strategies while maintaining animal welfare standards and
obtaining reliable diagnostic information.
PB  - Beograd : Fakultet veterinarske medicine, Univerzitet u Beogradu
PB  - Beograd : Veterinarska komora Srbije
T2  - Veterinarski glasnik
T1  - Overview of non-invasive sampling methods used in intensive swine farming
EP  - 108
IS  - 2
SP  - 97
VL  - 77
DO  - 10.2298/VETGL230614004G
ER  - 

@article{
author = "Glišić, Dimitrije and Veljović, Ljubiša and Milovanović, Bojan and Ninković, Milan and Maletić, Jelena and Kureljušić, Branislav and Milićević, Vesna",
year = "2023",
abstract = "Monitoring the health of swine herds is essential to ensure good manufacturing
practices. Traditionally, active and passive surveillance on farms involved invasive
sampling methods, where specific animals were selected, restrained, and sampled.
However, with the increasing intensity of swine production, alternative methods for
effective herd surveillance became necessary.
Non-invasive sampling provides a convenient and cost-effective approach to monitor
the entire herd without compromising animal welfare, while still obtaining suitable
samples for testing. Oral fluids have been widely used in both human and livestock
health surveillance for various viral pathogens, including significant diseases. Nasal
wipes (NW) utilize different cloth materials soaked in phosphate-buffered saline
(PBS) or tissue culture medium with antibiotics and antimycotics to sample for swine
influenza virus (SIV). Udder skin wipes (USW) offer an alternative method to assess
the health status of piglets in a litter. During routine procedures such as tail docking
and castration, a mixture of blood and serum can be collected, known as process fluids
(PF), which has proven successful in monitoring herds for the presence of porcine
reproductive and respiratory syndrome virus (PRRSV). Furthermore, air sampling has emerged as a novel technique to detect pathogens in various farming systems and
animal species. This method offers the advantage of obtaining diagnostic samples
without direct animal contact.
By employing these non-invasive sampling methods, swine producers can implement
effective surveillance strategies while maintaining animal welfare standards and
obtaining reliable diagnostic information.",
publisher = "Beograd : Fakultet veterinarske medicine, Univerzitet u Beogradu, Beograd : Veterinarska komora Srbije",
journal = "Veterinarski glasnik",
title = "Overview of non-invasive sampling methods used in intensive swine farming",
pages = "108-97",
number = "2",
volume = "77",
doi = "10.2298/VETGL230614004G"
}

Glišić, D., Veljović, L., Milovanović, B., Ninković, M., Maletić, J., Kureljušić, B.,& Milićević, V.. (2023). Overview of non-invasive sampling methods used in intensive swine farming. in Veterinarski glasnik
Beograd : Fakultet veterinarske medicine, Univerzitet u Beogradu., 77(2), 97-108.
https://doi.org/10.2298/VETGL230614004G

Glišić D, Veljović L, Milovanović B, Ninković M, Maletić J, Kureljušić B, Milićević V. Overview of non-invasive sampling methods used in intensive swine farming. in Veterinarski glasnik. 2023;77(2):97-108.
doi:10.2298/VETGL230614004G .

Glišić, Dimitrije, Veljović, Ljubiša, Milovanović, Bojan, Ninković, Milan, Maletić, Jelena, Kureljušić, Branislav, Milićević, Vesna, "Overview of non-invasive sampling methods used in intensive swine farming" in Veterinarski glasnik, 77, no. 2 (2023):97-108,
https://doi.org/10.2298/VETGL230614004G . .

TY  - CONF
AU  - Savić, Božidar
AU  - Kureljušić, Branislav
AU  - Milićević, Vesna
AU  - Jezdimirović, Nemanja
AU  - Milovanović, Bojan
AU  - Zdravković, Nemanja
AU  - Stevančević, Dimitrije
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/802
AB  - Porcine reproductive and respiratory syndrome virus CPRRSV) is the causative agent of a devastating pig disease worldwide, which is endemic in many countries, including the Serbia. Only limited epidemiological or molecular studies has been published on circulating PRRS-viruses in the Serbia since the early nineties. Therefore, the aim of this study is to investigate the genetic diversity of circulating PRRS-viruses in Serbia in the last ten years, by comparing the nucleotid€ sequences obtained for ORF5, wlth the ORF5 sequences of PRRSV 1 subtype 1.
PB  - ECPHM
C3  - Proceedings : ESPHM : 14th European symposium of porcine health management
T1  - Genetic diversity of the orf5 gene of type 1 porcine reproductive and respiratory syndrome virus isolates in Serbian swine herds from 2012 to 2022
EP  - 323
SP  - 323
ER  - 

@conference{
author = "Savić, Božidar and Kureljušić, Branislav and Milićević, Vesna and Jezdimirović, Nemanja and Milovanović, Bojan and Zdravković, Nemanja and Stevančević, Dimitrije",
year = "2023",
abstract = "Porcine reproductive and respiratory syndrome virus CPRRSV) is the causative agent of a devastating pig disease worldwide, which is endemic in many countries, including the Serbia. Only limited epidemiological or molecular studies has been published on circulating PRRS-viruses in the Serbia since the early nineties. Therefore, the aim of this study is to investigate the genetic diversity of circulating PRRS-viruses in Serbia in the last ten years, by comparing the nucleotid€ sequences obtained for ORF5, wlth the ORF5 sequences of PRRSV 1 subtype 1.",
publisher = "ECPHM",
journal = "Proceedings : ESPHM : 14th European symposium of porcine health management",
title = "Genetic diversity of the orf5 gene of type 1 porcine reproductive and respiratory syndrome virus isolates in Serbian swine herds from 2012 to 2022",
pages = "323-323"
}

Savić, B., Kureljušić, B., Milićević, V., Jezdimirović, N., Milovanović, B., Zdravković, N.,& Stevančević, D.. (2023). Genetic diversity of the orf5 gene of type 1 porcine reproductive and respiratory syndrome virus isolates in Serbian swine herds from 2012 to 2022. in Proceedings : ESPHM : 14th European symposium of porcine health management
ECPHM., 323-323.

Savić B, Kureljušić B, Milićević V, Jezdimirović N, Milovanović B, Zdravković N, Stevančević D. Genetic diversity of the orf5 gene of type 1 porcine reproductive and respiratory syndrome virus isolates in Serbian swine herds from 2012 to 2022. in Proceedings : ESPHM : 14th European symposium of porcine health management. 2023;:323-323..

Savić, Božidar, Kureljušić, Branislav, Milićević, Vesna, Jezdimirović, Nemanja, Milovanović, Bojan, Zdravković, Nemanja, Stevančević, Dimitrije, "Genetic diversity of the orf5 gene of type 1 porcine reproductive and respiratory syndrome virus isolates in Serbian swine herds from 2012 to 2022" in Proceedings : ESPHM : 14th European symposium of porcine health management (2023):323-323.

TY  - CONF
AU  - Milićević, Vesna
AU  - Glišić, Dimitrije
AU  - Milovanović, Bojan
AU  - Veljović, Ljubiša
AU  - Maletić, Jelena
AU  - Kureljušić, Branislav
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/803
AB  - Non-invasive sampling is increasingly used in domestic and wild animals. Traditional sampling methods are labour, time-consuming, and require trained personnel. Oral fluid is an alternative sample that can replace nasal swabs. Air sampling with special device and testing represents the newest method of non-invasive sampling, which is particularly useful and validated when testing pigs. Udder skin sampling is a new sampling approach that provides insight into litters of pigs before weaning, containing their nasal and oral secretions.
PB  - ECPHM
C3  - Proceedings : ESPHM : 14th European symposium of porcine health management
T1  - Porcine viral diseases can be successfully  monitored using non-invasive sampling strategy
EP  - 317
SP  - 317
ER  - 

@conference{
author = "Milićević, Vesna and Glišić, Dimitrije and Milovanović, Bojan and Veljović, Ljubiša and Maletić, Jelena and Kureljušić, Branislav",
year = "2023",
abstract = "Non-invasive sampling is increasingly used in domestic and wild animals. Traditional sampling methods are labour, time-consuming, and require trained personnel. Oral fluid is an alternative sample that can replace nasal swabs. Air sampling with special device and testing represents the newest method of non-invasive sampling, which is particularly useful and validated when testing pigs. Udder skin sampling is a new sampling approach that provides insight into litters of pigs before weaning, containing their nasal and oral secretions.",
publisher = "ECPHM",
journal = "Proceedings : ESPHM : 14th European symposium of porcine health management",
title = "Porcine viral diseases can be successfully  monitored using non-invasive sampling strategy",
pages = "317-317"
}

Milićević, V., Glišić, D., Milovanović, B., Veljović, L., Maletić, J.,& Kureljušić, B.. (2023). Porcine viral diseases can be successfully  monitored using non-invasive sampling strategy. in Proceedings : ESPHM : 14th European symposium of porcine health management
ECPHM., 317-317.

Milićević V, Glišić D, Milovanović B, Veljović L, Maletić J, Kureljušić B. Porcine viral diseases can be successfully  monitored using non-invasive sampling strategy. in Proceedings : ESPHM : 14th European symposium of porcine health management. 2023;:317-317..

Milićević, Vesna, Glišić, Dimitrije, Milovanović, Bojan, Veljović, Ljubiša, Maletić, Jelena, Kureljušić, Branislav, "Porcine viral diseases can be successfully  monitored using non-invasive sampling strategy" in Proceedings : ESPHM : 14th European symposium of porcine health management (2023):317-317.

TY  - CONF
AU  - Kureljušić, Branislav
AU  - Milićević, Vesna
AU  - Milovanović, Bojan
AU  - Glišić, Dimitrije
AU  - Maletić, Jelena
AU  - Jezdimirović, Nemanja
AU  - Kureljušić, Jasna
AU  - Prodanov Radulović, Jasna
AU  - Savić, Božidar
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/804
AB  - Biosecurity ls of paramount Importance to prevent the introduction and spread of pathogens in pig farms. Applied biosecurity measures and efficient on-farm management protect pigs from different infectious diseases. African swine fever CASF) is a severe, viral disease of domestic pigs and wild boar. After the occurrence in 2007 in Georgia, the disease gradually spread throughout Europe. The first case of ASF in Serbia was confirmed on July 30, 2019.
PB  - ECPHM
C3  - Proceedings : ESPHM : 14th European symposium of porcine health management
T1  - Assessment of biosecurity measures in the Serbian backyards where the first African swine fever cases were confirmed
EP  - 253
SP  - 253
ER  - 

@conference{
author = "Kureljušić, Branislav and Milićević, Vesna and Milovanović, Bojan and Glišić, Dimitrije and Maletić, Jelena and Jezdimirović, Nemanja and Kureljušić, Jasna and Prodanov Radulović, Jasna and Savić, Božidar",
year = "2023",
abstract = "Biosecurity ls of paramount Importance to prevent the introduction and spread of pathogens in pig farms. Applied biosecurity measures and efficient on-farm management protect pigs from different infectious diseases. African swine fever CASF) is a severe, viral disease of domestic pigs and wild boar. After the occurrence in 2007 in Georgia, the disease gradually spread throughout Europe. The first case of ASF in Serbia was confirmed on July 30, 2019.",
publisher = "ECPHM",
journal = "Proceedings : ESPHM : 14th European symposium of porcine health management",
title = "Assessment of biosecurity measures in the Serbian backyards where the first African swine fever cases were confirmed",
pages = "253-253"
}

Kureljušić, B., Milićević, V., Milovanović, B., Glišić, D., Maletić, J., Jezdimirović, N., Kureljušić, J., Prodanov Radulović, J.,& Savić, B.. (2023). Assessment of biosecurity measures in the Serbian backyards where the first African swine fever cases were confirmed. in Proceedings : ESPHM : 14th European symposium of porcine health management
ECPHM., 253-253.

Kureljušić B, Milićević V, Milovanović B, Glišić D, Maletić J, Jezdimirović N, Kureljušić J, Prodanov Radulović J, Savić B. Assessment of biosecurity measures in the Serbian backyards where the first African swine fever cases were confirmed. in Proceedings : ESPHM : 14th European symposium of porcine health management. 2023;:253-253..

Kureljušić, Branislav, Milićević, Vesna, Milovanović, Bojan, Glišić, Dimitrije, Maletić, Jelena, Jezdimirović, Nemanja, Kureljušić, Jasna, Prodanov Radulović, Jasna, Savić, Božidar, "Assessment of biosecurity measures in the Serbian backyards where the first African swine fever cases were confirmed" in Proceedings : ESPHM : 14th European symposium of porcine health management (2023):253-253.

TY  - JOUR
AU  - Milićević, Vesna
AU  - Glišić, Dimitrije
AU  - Veljović, Ljubiša
AU  - Vasić, Ana
AU  - Milovanović, Bojan
AU  - Kureljušić, Branislav
AU  - Paunović, Milan
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/777
AB  - Parvoviruses are among the major animal pathogens that can cause considerable health disorders ranging from subclinical
to lethal in domestic and wild animals. Golden jackal (Canis aureus), an expanding European species, is a reservoir of many
pathogens, including vector-borne diseases and zoonoses. Given the importance of parvovirus infections in dogs and cats,
this study aimed to unfold the virus prevalence and molecular characterisation in the golden jackal population in Serbia.
The spleen samples from 68 hunted jackals during 2022/2023 were tested for the VP2-specific genome region of Protoparvovirus
carnivoran 1 by PCR. BLAST analysis of partial VP2 sequences obtained from three animals (4.4%) revealed the
highest similarity to Protoparvovirus carnivoran 1, genogroup Feline panleukopenia virus, which is the second report on
FPV infection in jackals. Based on specific amino acid residues within partial VP2, the jackals’ Protoparvovirus carnivoran
1 was also classified as FPV. One jackal’s strain showed two synonymous mutations at positions 699 and 1167. Although
species cross-transmission could not be established, jackals’ health should be maintained by preventing the transmission
of viruses to native species and vice versa. Although jackals are considered pests, their role as natural cleaners is of greater
importance. Therefore, further monitoring of their health is needed to understand the influence of infectious diseases on
population dynamics and to determine the relationship between domestic predators and jackals and the direction of crossspecies
transmission.
PB  - Springer
T2  - Veterinary Research Communications
T1  - Protoparvovirus carnivoran 1 infection of golden jackals Canis aureus in Serbia
DO  - 10.1007/s11259-023-10249-0
ER  - 

@article{
author = "Milićević, Vesna and Glišić, Dimitrije and Veljović, Ljubiša and Vasić, Ana and Milovanović, Bojan and Kureljušić, Branislav and Paunović, Milan",
year = "2023",
abstract = "Parvoviruses are among the major animal pathogens that can cause considerable health disorders ranging from subclinical
to lethal in domestic and wild animals. Golden jackal (Canis aureus), an expanding European species, is a reservoir of many
pathogens, including vector-borne diseases and zoonoses. Given the importance of parvovirus infections in dogs and cats,
this study aimed to unfold the virus prevalence and molecular characterisation in the golden jackal population in Serbia.
The spleen samples from 68 hunted jackals during 2022/2023 were tested for the VP2-specific genome region of Protoparvovirus
carnivoran 1 by PCR. BLAST analysis of partial VP2 sequences obtained from three animals (4.4%) revealed the
highest similarity to Protoparvovirus carnivoran 1, genogroup Feline panleukopenia virus, which is the second report on
FPV infection in jackals. Based on specific amino acid residues within partial VP2, the jackals’ Protoparvovirus carnivoran
1 was also classified as FPV. One jackal’s strain showed two synonymous mutations at positions 699 and 1167. Although
species cross-transmission could not be established, jackals’ health should be maintained by preventing the transmission
of viruses to native species and vice versa. Although jackals are considered pests, their role as natural cleaners is of greater
importance. Therefore, further monitoring of their health is needed to understand the influence of infectious diseases on
population dynamics and to determine the relationship between domestic predators and jackals and the direction of crossspecies
transmission.",
publisher = "Springer",
journal = "Veterinary Research Communications",
title = "Protoparvovirus carnivoran 1 infection of golden jackals Canis aureus in Serbia",
doi = "10.1007/s11259-023-10249-0"
}

Milićević, V., Glišić, D., Veljović, L., Vasić, A., Milovanović, B., Kureljušić, B.,& Paunović, M.. (2023). Protoparvovirus carnivoran 1 infection of golden jackals Canis aureus in Serbia. in Veterinary Research Communications
Springer..
https://doi.org/10.1007/s11259-023-10249-0

Milićević V, Glišić D, Veljović L, Vasić A, Milovanović B, Kureljušić B, Paunović M. Protoparvovirus carnivoran 1 infection of golden jackals Canis aureus in Serbia. in Veterinary Research Communications. 2023;.
doi:10.1007/s11259-023-10249-0 .

Milićević, Vesna, Glišić, Dimitrije, Veljović, Ljubiša, Vasić, Ana, Milovanović, Bojan, Kureljušić, Branislav, Paunović, Milan, "Protoparvovirus carnivoran 1 infection of golden jackals Canis aureus in Serbia" in Veterinary Research Communications (2023),
https://doi.org/10.1007/s11259-023-10249-0 . .

TY  - JOUR
AU  - Milićević, Vesna
AU  - Glišić, Dimitrije
AU  - Zurovac Sapundžić, Zorana
AU  - Milovanović, Bojan
AU  - Maletić, Jelena
AU  - Jezdimirović, Nemanja
AU  - Kureljušić, Branislav
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/773
AB  - Contrary to pig farming in developedWestern countries, in a large part of the world, pigs
are still traditionally kept in small backyard farms, usually for family needs. Their main characteristics
are low biosecurity, swill feeding, natural breeding and uncontrolled trade. Given the high number
of backyard farms in Serbia and the risk they are thought to pose to intensive pig farming, the main
aim of this study was to evaluate the prevalence of major viral diseases of swine among traditionally
kept pigs in small holdings with low biosecurity. For this investigation, 222 serum samples from
69 backyard holdings were randomly selected and tested for antibodies to Porcine Reproductive and
Respiratory Syndrome virus (PRRSV), Aujeszky’s disease virus (ADV), Porcine Parvovirus (PPV)
and Swine influenza Virus (SIV) by enzyme-linked immunosorbent assay (ELISA). The herd-level
seroprevalence of PRRS, Aujeszky’s disease and PPV was 2.9%, 27.5% and 37.7%, respectively. Swine
influenza seroconversion was not confirmed in any of the tested holdings. Despite widely distributed
PPV and AD in backyard farms in Serbia, almost 50% of them are still negative for all the tested
diseases. The backyard farms must be monitored, and owners must be educated as their role in
eradication programs and obtaining country-free status may be crucial.
PB  - MDPI
T2  - Animals
T1  - Seroprevalence of viral enzootic diseases in swine backyard farms in Serbia
SP  - 3409
VL  - 13
DO  - 10.3390/ ani13213409
ER  - 

@article{
author = "Milićević, Vesna and Glišić, Dimitrije and Zurovac Sapundžić, Zorana and Milovanović, Bojan and Maletić, Jelena and Jezdimirović, Nemanja and Kureljušić, Branislav",
year = "2023",
abstract = "Contrary to pig farming in developedWestern countries, in a large part of the world, pigs
are still traditionally kept in small backyard farms, usually for family needs. Their main characteristics
are low biosecurity, swill feeding, natural breeding and uncontrolled trade. Given the high number
of backyard farms in Serbia and the risk they are thought to pose to intensive pig farming, the main
aim of this study was to evaluate the prevalence of major viral diseases of swine among traditionally
kept pigs in small holdings with low biosecurity. For this investigation, 222 serum samples from
69 backyard holdings were randomly selected and tested for antibodies to Porcine Reproductive and
Respiratory Syndrome virus (PRRSV), Aujeszky’s disease virus (ADV), Porcine Parvovirus (PPV)
and Swine influenza Virus (SIV) by enzyme-linked immunosorbent assay (ELISA). The herd-level
seroprevalence of PRRS, Aujeszky’s disease and PPV was 2.9%, 27.5% and 37.7%, respectively. Swine
influenza seroconversion was not confirmed in any of the tested holdings. Despite widely distributed
PPV and AD in backyard farms in Serbia, almost 50% of them are still negative for all the tested
diseases. The backyard farms must be monitored, and owners must be educated as their role in
eradication programs and obtaining country-free status may be crucial.",
publisher = "MDPI",
journal = "Animals",
title = "Seroprevalence of viral enzootic diseases in swine backyard farms in Serbia",
pages = "3409",
volume = "13",
doi = "10.3390/ ani13213409"
}

Milićević, V., Glišić, D., Zurovac Sapundžić, Z., Milovanović, B., Maletić, J., Jezdimirović, N.,& Kureljušić, B.. (2023). Seroprevalence of viral enzootic diseases in swine backyard farms in Serbia. in Animals
MDPI., 13, 3409.
https://doi.org/10.3390/ ani13213409

Milićević V, Glišić D, Zurovac Sapundžić Z, Milovanović B, Maletić J, Jezdimirović N, Kureljušić B. Seroprevalence of viral enzootic diseases in swine backyard farms in Serbia. in Animals. 2023;13:3409.
doi:10.3390/ ani13213409 .

Milićević, Vesna, Glišić, Dimitrije, Zurovac Sapundžić, Zorana, Milovanović, Bojan, Maletić, Jelena, Jezdimirović, Nemanja, Kureljušić, Branislav, "Seroprevalence of viral enzootic diseases in swine backyard farms in Serbia" in Animals, 13 (2023):3409,
https://doi.org/10.3390/ ani13213409 . .

TY  - CONF
AU  - Milićević, Vesna
AU  - Kureljušić, Branislav
AU  - Glišić, Dimitrije
AU  - Milovanović, Bojan
AU  - Veljović, Ljubiša
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/768
AB  - Foot-and-mouth disease (FMD) is a highly contagious viral disease of domestic
and wild ungulates and one of the most important diseases of animals in international
trade because it spreads very quickly and causes significant economic losses. In Africa,
Asia, the Middle East and a limited area of South America, FMD is an enzootic disease
and is controlled by vaccination. It is estimated that 77% of the world's livestock
population is affected by FMD (WOAH, 2022). Countries that are free of FMD without
vaccination are constantly at risk and invest heavily in preventing the introduction of
the virus. The causative agent of this disease is the FMD virus, which belongs to the
genus Aphtovirus and the family Picornaviridae. The virus is relatively resistant and
can survive in contaminated materials and the environment for several months under
ideal conditions. There are 7 serotypes and more than 60 subtypes of the FMD virus.
Immunity against one type of virus does not protect the animal from other serotypes
or subtypes.
The last cases of FMD in Europe were recorded in 2001 in the United Kingdom
(UK) and in 2011 in Bulgaria. FMD in the UK was registered on 2000 farms, as a result
of which about seven million animals were killed (Sutmoller et al., 2003). In Bulgaria,
the first case was detected in a wild boar on the border with Turkey, and then the
disease spread to domestic animals, cattle, sheep, goats and pigs on 11 farms. 2230 animals were killed in Bulgaria. Although sporadic cases of the disease were later
detected in wild game, it has not been proven that the FMD virus can be maintained in
wild animal populations (Elbers et al., 2003). The most common mechanism of the
spread of FMD is through direct contact, which can take the form of mechanical
transmission of the virus from infected to healthy animals through the skin and
mucous membranes or by aerosol and even by wind. The most important source of
infection is the contents of the vesicle, but the virus is also excreted in all
secretions/excreta, as well as in exhaled air. Cattle excrete less virus in their breath,
but, unlike pigs, they are very susceptible to infection through the respiratory route.
The minimum infectious dose of airborne virus for ruminants is only 10 TCID50, while
for pigs it is 103 TCID50 (Alekandersen et al., 2003). On the other hand, pigs are
considered an amplifier of the virus because they produce and excrete the most virus,
and therefore represent the greatest risk of spreading the disease. Depending on the
wind speed, infected pigs are a source of infection especially for cattle for miles
around. Such spread was noted in the UK when the disease was transferred from a pig
farm on the Isle of Wight to an area in northern France (Donaldson et al., 1982).
In addition to the pig phenomenon, a persistent form of infection in the
nasopharyngeal epithelium and lymphatic tissue is very important epizootiologically.
Persistent infection is defined as the presence of an infectious virus in the esophagealpharyngeal
(OP) fluid more than 15 or 21 days after infection in vaccinated or infected
cattle. The maximum duration of persistent infection in cattle is 3.5 years, sheep 9
months and goats 4 months (Alekandersen et al., 2003).
Clinically, the disease is characterized by a high body temperature (~
the appearance of vesicles in the oral cavity, feet and teats. After the bursting of the
vesicles, the processes become extremely painful, as a result of which the animals
cannot eat and avoid standing up if they have changes in their hooves. Most of the
affected animals recover, but with significant consequences, especially in milk
production. It is interesting that in young animals, sudden death can be the only sign
due to multifocal myocarditis that occurs before the appearance of vesicles. The
disease in sheep is mostly inapparent, which is why sheep are the species of choice for
serological surveillance of FMD.
In relation to the distribution of viruses, there are 7 enzootic areas (pools)
characterized by virus genotypes that circulate and develop independently within the
area. However, every year, the virus jumps from one area to another, usually through
illegal animal traffic. A very worrying epizootic event was recorded in early 2023 with
the emergence of SAT2/KSIV in Iraq, Jordan and Turkey (ADIS, 2023). The SAT2
epizootic was registered in three districts in Turkey on March 3, 2023, in the east of
the country. Out of 4944 susceptible animals, in all three districts, 7 clinical cases and
no deaths were determined. Analyzes of the nucleotide sequences of viruses from
these areas confirmed that the epizootics were caused by viruses of the SAT2/KSIV
topotype closely related to the viruses from Ethiopia in 2022. Considering that this is
the first occurrence of SAT2 in the mentioned countries where the population is fully
received, there is a justified concern from the rapid spread to other countries in the
region, the buffer zone without FMD in Thrace, as well as further west.
Turkey is the bridge that connects Europe and Asia and as such is very important
epizootiologically. The mass movements of animals in Turkey are always in the
direction of the west, towards the Marmara region and further towards Europe.
Foot and mouth disease is one of the most difficult animal diseases to control.
Different strategies can be applied to control FMD including mass vaccination as is the
case in Turkey.
Traditional measures to prevent the spread are the prohibition of traffic and trade
in animals and animal products from infected areas. Strict control at the border is a
crucial tactical measure because it has been shown that the legal and illegal movement
of animals contributes to the spread of diseases. In case of disease, the "stamping out"
method is applied, with the possibility of ring vaccination.
FMD is always a current topic because of its disastrous consequences in livestock
farming. In the future, due to rapid genetic change among serotypes, the virus will
continue to evolve and cause epizootics in enzootic areas. Therefore, trained
veterinary staff, good infrastructure, trained management and rapid diagnostic testing
in well-equipped laboratories must be ensured before the emergence of SIS. In
addition, the existence of an effective monitoring and surveillance system is crucial.
Serbia is a country free from FMD. Every year, for the purpose of confirming the free
status, surveillance is carried out by serological testing of a representative number of samples, Legislatively, SIŠ is regulated by the Ruleboook on establishing measures for
early detection, diagnosis, prevention of the spread, suppression and eradication of
foot-and-mouth disease ("Official Gazette of the RS", no. 15/2010) and the Rulebook
on establishing a crisis management plan (" Official Gazette of the RS", No. 90/2015)
PB  - Beograd : Srpsko veterinarsko društvo
C3  - 34. Savetovanje veterinara Srbije, Zlatibor, 7-10. septembar 2023
T1  - Slinavka i šap - bolest koja nam stalno preti
T1  - Foot and mouth disease - constantly threatening disease
EP  - 20
SP  - 16
ER  - 

@conference{
author = "Milićević, Vesna and Kureljušić, Branislav and Glišić, Dimitrije and Milovanović, Bojan and Veljović, Ljubiša",
year = "2023",
abstract = "Foot-and-mouth disease (FMD) is a highly contagious viral disease of domestic
and wild ungulates and one of the most important diseases of animals in international
trade because it spreads very quickly and causes significant economic losses. In Africa,
Asia, the Middle East and a limited area of South America, FMD is an enzootic disease
and is controlled by vaccination. It is estimated that 77% of the world's livestock
population is affected by FMD (WOAH, 2022). Countries that are free of FMD without
vaccination are constantly at risk and invest heavily in preventing the introduction of
the virus. The causative agent of this disease is the FMD virus, which belongs to the
genus Aphtovirus and the family Picornaviridae. The virus is relatively resistant and
can survive in contaminated materials and the environment for several months under
ideal conditions. There are 7 serotypes and more than 60 subtypes of the FMD virus.
Immunity against one type of virus does not protect the animal from other serotypes
or subtypes.
The last cases of FMD in Europe were recorded in 2001 in the United Kingdom
(UK) and in 2011 in Bulgaria. FMD in the UK was registered on 2000 farms, as a result
of which about seven million animals were killed (Sutmoller et al., 2003). In Bulgaria,
the first case was detected in a wild boar on the border with Turkey, and then the
disease spread to domestic animals, cattle, sheep, goats and pigs on 11 farms. 2230 animals were killed in Bulgaria. Although sporadic cases of the disease were later
detected in wild game, it has not been proven that the FMD virus can be maintained in
wild animal populations (Elbers et al., 2003). The most common mechanism of the
spread of FMD is through direct contact, which can take the form of mechanical
transmission of the virus from infected to healthy animals through the skin and
mucous membranes or by aerosol and even by wind. The most important source of
infection is the contents of the vesicle, but the virus is also excreted in all
secretions/excreta, as well as in exhaled air. Cattle excrete less virus in their breath,
but, unlike pigs, they are very susceptible to infection through the respiratory route.
The minimum infectious dose of airborne virus for ruminants is only 10 TCID50, while
for pigs it is 103 TCID50 (Alekandersen et al., 2003). On the other hand, pigs are
considered an amplifier of the virus because they produce and excrete the most virus,
and therefore represent the greatest risk of spreading the disease. Depending on the
wind speed, infected pigs are a source of infection especially for cattle for miles
around. Such spread was noted in the UK when the disease was transferred from a pig
farm on the Isle of Wight to an area in northern France (Donaldson et al., 1982).
In addition to the pig phenomenon, a persistent form of infection in the
nasopharyngeal epithelium and lymphatic tissue is very important epizootiologically.
Persistent infection is defined as the presence of an infectious virus in the esophagealpharyngeal
(OP) fluid more than 15 or 21 days after infection in vaccinated or infected
cattle. The maximum duration of persistent infection in cattle is 3.5 years, sheep 9
months and goats 4 months (Alekandersen et al., 2003).
Clinically, the disease is characterized by a high body temperature (~
the appearance of vesicles in the oral cavity, feet and teats. After the bursting of the
vesicles, the processes become extremely painful, as a result of which the animals
cannot eat and avoid standing up if they have changes in their hooves. Most of the
affected animals recover, but with significant consequences, especially in milk
production. It is interesting that in young animals, sudden death can be the only sign
due to multifocal myocarditis that occurs before the appearance of vesicles. The
disease in sheep is mostly inapparent, which is why sheep are the species of choice for
serological surveillance of FMD.
In relation to the distribution of viruses, there are 7 enzootic areas (pools)
characterized by virus genotypes that circulate and develop independently within the
area. However, every year, the virus jumps from one area to another, usually through
illegal animal traffic. A very worrying epizootic event was recorded in early 2023 with
the emergence of SAT2/KSIV in Iraq, Jordan and Turkey (ADIS, 2023). The SAT2
epizootic was registered in three districts in Turkey on March 3, 2023, in the east of
the country. Out of 4944 susceptible animals, in all three districts, 7 clinical cases and
no deaths were determined. Analyzes of the nucleotide sequences of viruses from
these areas confirmed that the epizootics were caused by viruses of the SAT2/KSIV
topotype closely related to the viruses from Ethiopia in 2022. Considering that this is
the first occurrence of SAT2 in the mentioned countries where the population is fully
received, there is a justified concern from the rapid spread to other countries in the
region, the buffer zone without FMD in Thrace, as well as further west.
Turkey is the bridge that connects Europe and Asia and as such is very important
epizootiologically. The mass movements of animals in Turkey are always in the
direction of the west, towards the Marmara region and further towards Europe.
Foot and mouth disease is one of the most difficult animal diseases to control.
Different strategies can be applied to control FMD including mass vaccination as is the
case in Turkey.
Traditional measures to prevent the spread are the prohibition of traffic and trade
in animals and animal products from infected areas. Strict control at the border is a
crucial tactical measure because it has been shown that the legal and illegal movement
of animals contributes to the spread of diseases. In case of disease, the "stamping out"
method is applied, with the possibility of ring vaccination.
FMD is always a current topic because of its disastrous consequences in livestock
farming. In the future, due to rapid genetic change among serotypes, the virus will
continue to evolve and cause epizootics in enzootic areas. Therefore, trained
veterinary staff, good infrastructure, trained management and rapid diagnostic testing
in well-equipped laboratories must be ensured before the emergence of SIS. In
addition, the existence of an effective monitoring and surveillance system is crucial.
Serbia is a country free from FMD. Every year, for the purpose of confirming the free
status, surveillance is carried out by serological testing of a representative number of samples, Legislatively, SIŠ is regulated by the Ruleboook on establishing measures for
early detection, diagnosis, prevention of the spread, suppression and eradication of
foot-and-mouth disease ("Official Gazette of the RS", no. 15/2010) and the Rulebook
on establishing a crisis management plan (" Official Gazette of the RS", No. 90/2015)",
publisher = "Beograd : Srpsko veterinarsko društvo",
journal = "34. Savetovanje veterinara Srbije, Zlatibor, 7-10. septembar 2023",
title = "Slinavka i šap - bolest koja nam stalno preti, Foot and mouth disease - constantly threatening disease",
pages = "20-16"
}

Milićević, V., Kureljušić, B., Glišić, D., Milovanović, B.,& Veljović, L.. (2023). Slinavka i šap - bolest koja nam stalno preti. in 34. Savetovanje veterinara Srbije, Zlatibor, 7-10. septembar 2023
Beograd : Srpsko veterinarsko društvo., 16-20.

Milićević V, Kureljušić B, Glišić D, Milovanović B, Veljović L. Slinavka i šap - bolest koja nam stalno preti. in 34. Savetovanje veterinara Srbije, Zlatibor, 7-10. septembar 2023. 2023;:16-20..

Milićević, Vesna, Kureljušić, Branislav, Glišić, Dimitrije, Milovanović, Bojan, Veljović, Ljubiša, "Slinavka i šap - bolest koja nam stalno preti" in 34. Savetovanje veterinara Srbije, Zlatibor, 7-10. septembar 2023 (2023):16-20.

TY  - CONF
AU  - Maletić, Jelena
AU  - Kureljušić, Jasna
AU  - Milovanović, Bojan
AU  - Milićević, Vesna
AU  - Radosavljevic, Vladimir
AU  - Spalević, Ljiljana
AU  - Kureljušić, Branislav
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/551
AB  - Biosigurnost podrazumeva primenu određenih preventivnih mera na farmi u
cilju smanjenja mogućnosti unošenja i širenja patogenih mikroorganizama. Biosigurnost može biti spoljašnja (eksterna) i unutrašnja (interna). Procena biosigurnosti predstavlja kvalitativno ili kvantitativno određivanje nivoa biosigurnosti na
farmama. Istraživanje je sprovedeno na dve brojlerske farme na kojima je procena nivoa biosigurnosti sprovedena upotrebom on-lajn upitnika – Biocheck.Ugent.
Ukupna ocena biosigurnosti na farmi 1 bila je viša za 22% u odnosu na ocenu za
farmu 2. Ocena eksterne biosigurnosti na farmi 1 je za 27% viša u odnosu na ocenu za farmu 2, dok su se ocene za internu biosigurnost razlikovale za 8%. Takođe,
utvrđno je da su ocena eksterne biosigurnosti, kao i ukupna ocena za biosigurnosne mere koje se sprovode na farmi 2, niže u odnosu na svetski prosek dobijen iz
baze podataka Biochek.UGent-a. Dobijene ocene za potkategorije, razlikovale su
se između farmi. Najniže ocene za eksternu biosigurnost dobile su potkategorije
„uklanjanje stajnjaka i leševa” (5/100), „snabdevanje hranom i vodom” (36/100) i
„depopulacija pilića” (51/100), a za internu potkategoriju „čišćenje i dezinfekcija”
(37/100). Sistem za procenu implentiranih biosigurnosnih mera može se posmatrati kao vredan instrument za praćenje nivoa biosigurnosti. Periodična procena
biosigurnosti na brojlerskim farmama na nivou cele zemlje, omogućila bi mapiranje farmi prema nivou biosigurnosti i prema riziku od izbijanja određene bolesti.
AB  - Biosecurity implies the application of certain preventive measures on the farm
in order to reduce the possibility of the introduction and spread of pathogenic
microorganisms. Biosecurity can be external and internal. Biosecurity assessment
is a qualitative or quantitative determination of the level of biosecurity on farms.
The study was conducted on two broiler farms where the biosecurity assessment
was carried out using an online questionnaire – Biocheck.Ugent. The overall biosecurity score on farm 1 was 22% higher than that for farm 2. The external biosecurity score on farm 1 was 27% higher than that for farm 2, while the internal
biosecurity score differed by 8%. The external biosecurity score on farm 2 was
lower than the internal biosecurity score. Also, it was determined that the external
biosecurity, as well as the overall biosecurity implemented on farm 2, had a lower
score compared to the world average score obtained from Biochek.UGent database. Scores obtained for subcategories differed between farms. The lowest scores
for external biosecurity were for the subcategories ”manure and carcass removal” (5/100), ”food and water supply” (36/100), and ”depopulation” (51/100), and
for the internal subcategory ”cleaning and disinfection” (37/100). The system for
evaluating of implemented biosecurity measures can be seen as a valuable instrument for monitoring the level of biosecurity. A periodic assessment of biosecurity
on broiler farms at the country level would enable the mapping of farms according to the level of biosecurity and according to the risk of the outbreak of a certain
disease.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za DDD
PB  - Beograd : Fakultet veterinarske medicine, Katedra za zoohigijenu
C3  - 34. Savetovanje Dezinfekcija, dezinsekcija i deratizacija : Jedan svet  - jedno zdravlje
T1  - Značaj procene nivoa biosigurnosti na brojlerskim farmama
T1  - The importance of biosecurity assessment on the broiler farms
EP  - 109
SP  - 102
ER  - 

@conference{
author = "Maletić, Jelena and Kureljušić, Jasna and Milovanović, Bojan and Milićević, Vesna and Radosavljevic, Vladimir and Spalević, Ljiljana and Kureljušić, Branislav",
year = "2023",
abstract = "Biosigurnost podrazumeva primenu određenih preventivnih mera na farmi u
cilju smanjenja mogućnosti unošenja i širenja patogenih mikroorganizama. Biosigurnost može biti spoljašnja (eksterna) i unutrašnja (interna). Procena biosigurnosti predstavlja kvalitativno ili kvantitativno određivanje nivoa biosigurnosti na
farmama. Istraživanje je sprovedeno na dve brojlerske farme na kojima je procena nivoa biosigurnosti sprovedena upotrebom on-lajn upitnika – Biocheck.Ugent.
Ukupna ocena biosigurnosti na farmi 1 bila je viša za 22% u odnosu na ocenu za
farmu 2. Ocena eksterne biosigurnosti na farmi 1 je za 27% viša u odnosu na ocenu za farmu 2, dok su se ocene za internu biosigurnost razlikovale za 8%. Takođe,
utvrđno je da su ocena eksterne biosigurnosti, kao i ukupna ocena za biosigurnosne mere koje se sprovode na farmi 2, niže u odnosu na svetski prosek dobijen iz
baze podataka Biochek.UGent-a. Dobijene ocene za potkategorije, razlikovale su
se između farmi. Najniže ocene za eksternu biosigurnost dobile su potkategorije
„uklanjanje stajnjaka i leševa” (5/100), „snabdevanje hranom i vodom” (36/100) i
„depopulacija pilića” (51/100), a za internu potkategoriju „čišćenje i dezinfekcija”
(37/100). Sistem za procenu implentiranih biosigurnosnih mera može se posmatrati kao vredan instrument za praćenje nivoa biosigurnosti. Periodična procena
biosigurnosti na brojlerskim farmama na nivou cele zemlje, omogućila bi mapiranje farmi prema nivou biosigurnosti i prema riziku od izbijanja određene bolesti., Biosecurity implies the application of certain preventive measures on the farm
in order to reduce the possibility of the introduction and spread of pathogenic
microorganisms. Biosecurity can be external and internal. Biosecurity assessment
is a qualitative or quantitative determination of the level of biosecurity on farms.
The study was conducted on two broiler farms where the biosecurity assessment
was carried out using an online questionnaire – Biocheck.Ugent. The overall biosecurity score on farm 1 was 22% higher than that for farm 2. The external biosecurity score on farm 1 was 27% higher than that for farm 2, while the internal
biosecurity score differed by 8%. The external biosecurity score on farm 2 was
lower than the internal biosecurity score. Also, it was determined that the external
biosecurity, as well as the overall biosecurity implemented on farm 2, had a lower
score compared to the world average score obtained from Biochek.UGent database. Scores obtained for subcategories differed between farms. The lowest scores
for external biosecurity were for the subcategories ”manure and carcass removal” (5/100), ”food and water supply” (36/100), and ”depopulation” (51/100), and
for the internal subcategory ”cleaning and disinfection” (37/100). The system for
evaluating of implemented biosecurity measures can be seen as a valuable instrument for monitoring the level of biosecurity. A periodic assessment of biosecurity
on broiler farms at the country level would enable the mapping of farms according to the level of biosecurity and according to the risk of the outbreak of a certain
disease.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za DDD, Beograd : Fakultet veterinarske medicine, Katedra za zoohigijenu",
journal = "34. Savetovanje Dezinfekcija, dezinsekcija i deratizacija : Jedan svet  - jedno zdravlje",
title = "Značaj procene nivoa biosigurnosti na brojlerskim farmama, The importance of biosecurity assessment on the broiler farms",
pages = "109-102"
}

Maletić, J., Kureljušić, J., Milovanović, B., Milićević, V., Radosavljevic, V., Spalević, L.,& Kureljušić, B.. (2023). Značaj procene nivoa biosigurnosti na brojlerskim farmama. in 34. Savetovanje Dezinfekcija, dezinsekcija i deratizacija : Jedan svet  - jedno zdravlje
Beograd : Srpsko veterinarsko društvo, Sekcija za DDD., 102-109.

Maletić J, Kureljušić J, Milovanović B, Milićević V, Radosavljevic V, Spalević L, Kureljušić B. Značaj procene nivoa biosigurnosti na brojlerskim farmama. in 34. Savetovanje Dezinfekcija, dezinsekcija i deratizacija : Jedan svet  - jedno zdravlje. 2023;:102-109..

Maletić, Jelena, Kureljušić, Jasna, Milovanović, Bojan, Milićević, Vesna, Radosavljevic, Vladimir, Spalević, Ljiljana, Kureljušić, Branislav, "Značaj procene nivoa biosigurnosti na brojlerskim farmama" in 34. Savetovanje Dezinfekcija, dezinsekcija i deratizacija : Jedan svet  - jedno zdravlje (2023):102-109.

TY  - CONF
AU  - Jezdimirović, Nemanja
AU  - Kureljušić, Branislav
AU  - Savić, Božidar
AU  - Milovanović, Bojan
AU  - Glišić, Dimitrije
AU  - Maksimović Zorić, Jelena
AU  - Milićević, Vesna
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/767
AB  - Kliničko oboljenje uzrokovano sa porcine citomegalovirus (PCMV) se javlja samo
kod svinja, iako se infekcije srodnim virusima pojavljuju i kod drugih životinjskih vrsta
uključujući i ljude. Citomegalovirus kod svinja uzrokuje rinitis sa inkluzionim
telašcima (IBR), PCMV pripada podfamiliji Betaherpesvirinae i familiji Herpesviridae,
ali nije svrstan ni u jedan rod, a genetska istraživanja su pokazala da je PCMV genetski
srodniji humanim herpes virusima 6 i 7. Infekcija sa PCMV je prisutna širom sveta,
dominantno u zemljama sa intenzivnom proizvodnjom svinja i uglavnom je klinički
inaparentna zbog stečenog imuniteta zapata. Virus se prenosi horizontalno
(oronazalnim putem), a moguća je i kongenitalna transmisija, pa inficirani fetusi mogu
biti, mumificirani, mrtvorođeni ili rođeni živi kao slabo vitalna prasad. Kliničko
oboljenje se pojavljuje u akutnoj ili subakutnoj formi kod prasadi starosti do 4 nedelje,
a može se pojaviti i kod drugih starosnih kategorija u neimunim zapatima. Svinje koje
su prebolele infekciju ostaju doživotne kliconoše. Suide predstavljaju jedini rezervoar
virusa u prirodi. Rinitis sa inkluzionim telašcima je visoko prevalentna infekcija i
pretpostavlja se da je prevalencija oboljenja preko 90% na globalnom nivou, odnosno
98% u Evropi. U našoj zemlji još uvek nisu rađena opsežna istraživanja na utvrđivanju
prevalence oboljenja zbog čega su prevalenca infekcije i incidenca oboljenja još uvek
nepoznate. Zbog cirkulacije drugih respiratornih virusa u farmskim uslovima uzgoja
svinja, PCMV je često zapostavljen. Na poziv veterinarske službe, na farmi koja je imala učestale probleme sa
respiratornim oboljenjima, izvršili smo uzorkovanje oralnog fluida i analizirali
prikupljene uzorke primenom lančane reakcije polimeraze na prisustvo sledećih
virusnih agenasa: Encephalomyocarditis virus (EMCV), PCMV, Porcine circovirus tip 2
(PCV2). Virus reproduktivnog i respiratornog sindroma svinja (PRRSV) i Virus
influence svinja (SIV), sa ciljem uvida u prisustvo virusnih infektivnih agenasa na
farmi. Izvršenim analizama smo pored detekcije PRRSV, PCV2 i SIV, ustanovili i
prisustvo genoma PCMV kod prasadi iz odgoja. Mortalitet prasadi u neimunim
zapatima od posledica kliničkog PCMV-rinitisa može dostići 10%, ali se može povećati
do čak 50% ukoliko se bolest komplikuje drugim virusnim ili bakterijskim infekcijama.
Klinički značaj prisustva PCMV u konkretnom slučaju bi trebalo razmatrati u
kontekstu detekcije drugih virusnih patogena i njihovog sinergističkog efekta.
Međutim, obzirom da PCMV samostalno ili u koinfekcijama sa drugim, pre svega
virusnim respiratornim agensima može biti uzrok povećanja mortaliteta, ne treba ga
zanemariti. Vakcina protiv PCMV i specifična terapija ovog oboljenja ne postoje, sa
druge strane, ne postojanje informacija o prevalenci PCMV u našoj zemlji kao ni
vršenje rutinskog pregleda na ovu infekciju, predstavlja značajan rizik za širenje
virusa pre svega uvođenjem novonabavljenih životinja u zapat.
PB  - Beograd : Srpsko veterinarsko društvo
C3  - 34. Savetovanje veterinara Srbije, Zlatibor,  7-10. septembar 2023
T1  - Prva molekularna detekcija citomegalovirusa svinja u Srbiji
EP  - 95
SP  - 90
ER  - 

@conference{
author = "Jezdimirović, Nemanja and Kureljušić, Branislav and Savić, Božidar and Milovanović, Bojan and Glišić, Dimitrije and Maksimović Zorić, Jelena and Milićević, Vesna",
year = "2023",
abstract = "Kliničko oboljenje uzrokovano sa porcine citomegalovirus (PCMV) se javlja samo
kod svinja, iako se infekcije srodnim virusima pojavljuju i kod drugih životinjskih vrsta
uključujući i ljude. Citomegalovirus kod svinja uzrokuje rinitis sa inkluzionim
telašcima (IBR), PCMV pripada podfamiliji Betaherpesvirinae i familiji Herpesviridae,
ali nije svrstan ni u jedan rod, a genetska istraživanja su pokazala da je PCMV genetski
srodniji humanim herpes virusima 6 i 7. Infekcija sa PCMV je prisutna širom sveta,
dominantno u zemljama sa intenzivnom proizvodnjom svinja i uglavnom je klinički
inaparentna zbog stečenog imuniteta zapata. Virus se prenosi horizontalno
(oronazalnim putem), a moguća je i kongenitalna transmisija, pa inficirani fetusi mogu
biti, mumificirani, mrtvorođeni ili rođeni živi kao slabo vitalna prasad. Kliničko
oboljenje se pojavljuje u akutnoj ili subakutnoj formi kod prasadi starosti do 4 nedelje,
a može se pojaviti i kod drugih starosnih kategorija u neimunim zapatima. Svinje koje
su prebolele infekciju ostaju doživotne kliconoše. Suide predstavljaju jedini rezervoar
virusa u prirodi. Rinitis sa inkluzionim telašcima je visoko prevalentna infekcija i
pretpostavlja se da je prevalencija oboljenja preko 90% na globalnom nivou, odnosno
98% u Evropi. U našoj zemlji još uvek nisu rađena opsežna istraživanja na utvrđivanju
prevalence oboljenja zbog čega su prevalenca infekcije i incidenca oboljenja još uvek
nepoznate. Zbog cirkulacije drugih respiratornih virusa u farmskim uslovima uzgoja
svinja, PCMV je često zapostavljen. Na poziv veterinarske službe, na farmi koja je imala učestale probleme sa
respiratornim oboljenjima, izvršili smo uzorkovanje oralnog fluida i analizirali
prikupljene uzorke primenom lančane reakcije polimeraze na prisustvo sledećih
virusnih agenasa: Encephalomyocarditis virus (EMCV), PCMV, Porcine circovirus tip 2
(PCV2). Virus reproduktivnog i respiratornog sindroma svinja (PRRSV) i Virus
influence svinja (SIV), sa ciljem uvida u prisustvo virusnih infektivnih agenasa na
farmi. Izvršenim analizama smo pored detekcije PRRSV, PCV2 i SIV, ustanovili i
prisustvo genoma PCMV kod prasadi iz odgoja. Mortalitet prasadi u neimunim
zapatima od posledica kliničkog PCMV-rinitisa može dostići 10%, ali se može povećati
do čak 50% ukoliko se bolest komplikuje drugim virusnim ili bakterijskim infekcijama.
Klinički značaj prisustva PCMV u konkretnom slučaju bi trebalo razmatrati u
kontekstu detekcije drugih virusnih patogena i njihovog sinergističkog efekta.
Međutim, obzirom da PCMV samostalno ili u koinfekcijama sa drugim, pre svega
virusnim respiratornim agensima može biti uzrok povećanja mortaliteta, ne treba ga
zanemariti. Vakcina protiv PCMV i specifična terapija ovog oboljenja ne postoje, sa
druge strane, ne postojanje informacija o prevalenci PCMV u našoj zemlji kao ni
vršenje rutinskog pregleda na ovu infekciju, predstavlja značajan rizik za širenje
virusa pre svega uvođenjem novonabavljenih životinja u zapat.",
publisher = "Beograd : Srpsko veterinarsko društvo",
journal = "34. Savetovanje veterinara Srbije, Zlatibor,  7-10. septembar 2023",
title = "Prva molekularna detekcija citomegalovirusa svinja u Srbiji",
pages = "95-90"
}

Jezdimirović, N., Kureljušić, B., Savić, B., Milovanović, B., Glišić, D., Maksimović Zorić, J.,& Milićević, V.. (2023). Prva molekularna detekcija citomegalovirusa svinja u Srbiji. in 34. Savetovanje veterinara Srbije, Zlatibor,  7-10. septembar 2023
Beograd : Srpsko veterinarsko društvo., 90-95.

Jezdimirović N, Kureljušić B, Savić B, Milovanović B, Glišić D, Maksimović Zorić J, Milićević V. Prva molekularna detekcija citomegalovirusa svinja u Srbiji. in 34. Savetovanje veterinara Srbije, Zlatibor,  7-10. septembar 2023. 2023;:90-95..

Jezdimirović, Nemanja, Kureljušić, Branislav, Savić, Božidar, Milovanović, Bojan, Glišić, Dimitrije, Maksimović Zorić, Jelena, Milićević, Vesna, "Prva molekularna detekcija citomegalovirusa svinja u Srbiji" in 34. Savetovanje veterinara Srbije, Zlatibor,  7-10. septembar 2023 (2023):90-95.

TY  - CONF
AU  - Stanojević, Slobodan
AU  - Vojinović, Dragica
AU  - Zdravković, Nemanja
AU  - Milovanović, Bojan
AU  - Žutić, Jadranka
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/815
AB  - Q groznica je zoonoza rasprostranjena u celom svetu osim na Novom Zelandu,
predstavlja znaĉajan uzrok oboljevanja ljudi širom sveta. Bolest izaziva Coxiella
burnetii, intracelularna, Gram-negativna bekterija. Endemski je prisutna u
Srbiji ali uprkos ĉinjenici da je vaţna za javno zdravlje i ekonomiju stanovišta,
o njoj se malo izveštava dok je nadzor slabo razvijen. Postoji veliki broj rezervoara
u prirodi koje uglavnom ĉine sisari, ptice i krpelji. Domaći preţivari se
smatraju glavnim izvorom infekcije od kojih se moţe zaraziti veliki broj drugih
domaćina. Serološke studije koje smo sproveli pokazale su prisustvo antitela na
C. burnetii u Beogradskoj epizootiološkoj oblasti. Seroprevalencija kod goveda
na farmama je iznosila 18%, dok je u porodiĉnim gazdinstvima bila 1,5%. Kod
krava na farmama koje su pobacile prevalencija je iznoslila 49%, dok je u
porodiĉnim gazdinstvima bila 1,9%. Infekcija kod domaćih ţivotinja najĉešće
prolazi asimptomatski i obiĉno se primeti tek kada nastanu reproduktivni
poremećaji i pobaĉaji. Tokom abortusa i poroĊaja obolele ţivotinje izluĉuju u
spoljašnju sredinu veliku koliĉinu bakterija, i do 1 milijarde po gramu placente.
Pojave epidemija kod ljudi najĉešće su povezane sa klanicama i farmama sa
intenzivnim gajenjam ovaca i koza. Kliniĉki Q groznica se i kod ljudi naĉešće
javlja asimptomatska, ali i u formi nespecifiĉne groznice, atipiĉne pneumonije,
hepatitisa i endokarditisa. Pošto su kliniĉki znaci bolesti nespecifiĉni, laboratorijski
dokazi infekcije su neophodni za postavljanje taĉne dijagnoze i otpoĉinjanje
specifiĉne terapije. Bolest se leĉi upotrebom specifiĉnih antibiotika, a
kod neleĉenih pacijenata stopa smrtnosti moţe biti visoka. Q groznica se
uglavnom prenosi udisanjem kontaminiranog aerosola, kontaktom sa obolelim
ţivotinjama i kontaminiranom okolinom ili konzumiranjem zaraţenog mleka i
ţivotinjskih proizvoda. Prevencija je usmerena na izbegavanje izlaganja ljudi,
posebno riziĉnih osoba, ţivotinjama i zagaĊenoj ţivotnoj sredini. Dobrim upravljanjem
procesima na farmama sniţava se stepen kontaminacije okoline i
spreĉavaju kontakti ljudi i ţivotinja sa izvorima infekcije. Poboljšanja u 
zoru i izveštavanje o infekcijama kod ţivotinja omogućava blagovremeno reagovanje
veterinarskih i zdravstevnih sluţbi, kao sastavnih delova sistema jednog
zdravlja, ĉime se postiţe veća efikasnost u kontroli i spreĉavanju nastanka Q
groznice i njenom blagovremenom leĉenju.
AB  - Q fever is a zoonosis spread throughout the world except in New Zealand, and
presents a significant cause of human illness worldwide. The disease is caused
by Coxiella burnetii, an intracellular, Gram-negative bacterium. It is endemic
in Serbia, but despite the fact that it is important for public health and economy,
it is reported rarely and surveillance is poorly developed. There are numerous
reservoirs in nature, which are mainly mammals, birds and ticks. Domestic
ruminants are considered the main source of infection from which a large
number of different hosts can be infected. The serological studies we conducted
showed the presence of antibodies to C. burnetii in the Belgrade epizootic area.
Seroprevalence in cattle on intensive farms was 18%, while in family farms it
was 1.5%. In cows that aborted on farms, the prevalence was 49%, while in
family farms it was 1.9%. Infection in domestic animals is usually asymptomatic
and usually noticed only when reproductive disorders and abortions occur.
During abortion and labor, infected animals excrete large amount of bacteria
into the external environment, up to 1 billion per gram of placenta. Epidemics
in humans are most often associated with slaughterhouses and farms with
intensive breeding of sheep and goats. Clinically, Q fever is often asymptomatic
in humans, but also occures in the form of non-specific fever, atypical
pneumonia, hepatitis and endocarditis. Since the clinical signs of the disease
are nonspecific, laboratory evidence of infection is necessary to establish an
accurate diagnosis and initiate specific therapy. The disease is treated with the
use of specific antibiotics, and in untreated patients the mortality rate can be
high. Q fever is mainly transmitted by inhalation of contaminated aerosols,
contact with infected animals and contaminated environment, or by consumption
of infected milk and animal products. Prevention is directed to avoid
exposure of people, especially persons at risk, to animals and the polluted environment.
Good process management on farms lowers the level of environmental
contamination and prevents people and animals from coming into contact with
sources of infection. Improvements of the monitoring and reporting of infections
in animals enable the timely response of veterinary and health services, as
integral parts of one health system, thus achieving greater efficiency in controlling
and preventing the occurrence of Q fever and allowing the timely
treatment.
PB  - Beograd : Fakultet veterinarske medicine
C3  - Četvrti regionalni simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 29. jun - 1. jul 2023
T1  - Epizootiologija Q groznice i njen društveno ekonomski uticaj i implikacije na javno zdravlje
T1  - Epizootiology of Q fever, its socioeconomic impact, and public health implications
EP  - 206
SP  - 191
ER  - 

@conference{
author = "Stanojević, Slobodan and Vojinović, Dragica and Zdravković, Nemanja and Milovanović, Bojan and Žutić, Jadranka",
year = "2023",
abstract = "Q groznica je zoonoza rasprostranjena u celom svetu osim na Novom Zelandu,
predstavlja znaĉajan uzrok oboljevanja ljudi širom sveta. Bolest izaziva Coxiella
burnetii, intracelularna, Gram-negativna bekterija. Endemski je prisutna u
Srbiji ali uprkos ĉinjenici da je vaţna za javno zdravlje i ekonomiju stanovišta,
o njoj se malo izveštava dok je nadzor slabo razvijen. Postoji veliki broj rezervoara
u prirodi koje uglavnom ĉine sisari, ptice i krpelji. Domaći preţivari se
smatraju glavnim izvorom infekcije od kojih se moţe zaraziti veliki broj drugih
domaćina. Serološke studije koje smo sproveli pokazale su prisustvo antitela na
C. burnetii u Beogradskoj epizootiološkoj oblasti. Seroprevalencija kod goveda
na farmama je iznosila 18%, dok je u porodiĉnim gazdinstvima bila 1,5%. Kod
krava na farmama koje su pobacile prevalencija je iznoslila 49%, dok je u
porodiĉnim gazdinstvima bila 1,9%. Infekcija kod domaćih ţivotinja najĉešće
prolazi asimptomatski i obiĉno se primeti tek kada nastanu reproduktivni
poremećaji i pobaĉaji. Tokom abortusa i poroĊaja obolele ţivotinje izluĉuju u
spoljašnju sredinu veliku koliĉinu bakterija, i do 1 milijarde po gramu placente.
Pojave epidemija kod ljudi najĉešće su povezane sa klanicama i farmama sa
intenzivnim gajenjam ovaca i koza. Kliniĉki Q groznica se i kod ljudi naĉešće
javlja asimptomatska, ali i u formi nespecifiĉne groznice, atipiĉne pneumonije,
hepatitisa i endokarditisa. Pošto su kliniĉki znaci bolesti nespecifiĉni, laboratorijski
dokazi infekcije su neophodni za postavljanje taĉne dijagnoze i otpoĉinjanje
specifiĉne terapije. Bolest se leĉi upotrebom specifiĉnih antibiotika, a
kod neleĉenih pacijenata stopa smrtnosti moţe biti visoka. Q groznica se
uglavnom prenosi udisanjem kontaminiranog aerosola, kontaktom sa obolelim
ţivotinjama i kontaminiranom okolinom ili konzumiranjem zaraţenog mleka i
ţivotinjskih proizvoda. Prevencija je usmerena na izbegavanje izlaganja ljudi,
posebno riziĉnih osoba, ţivotinjama i zagaĊenoj ţivotnoj sredini. Dobrim upravljanjem
procesima na farmama sniţava se stepen kontaminacije okoline i
spreĉavaju kontakti ljudi i ţivotinja sa izvorima infekcije. Poboljšanja u 
zoru i izveštavanje o infekcijama kod ţivotinja omogućava blagovremeno reagovanje
veterinarskih i zdravstevnih sluţbi, kao sastavnih delova sistema jednog
zdravlja, ĉime se postiţe veća efikasnost u kontroli i spreĉavanju nastanka Q
groznice i njenom blagovremenom leĉenju., Q fever is a zoonosis spread throughout the world except in New Zealand, and
presents a significant cause of human illness worldwide. The disease is caused
by Coxiella burnetii, an intracellular, Gram-negative bacterium. It is endemic
in Serbia, but despite the fact that it is important for public health and economy,
it is reported rarely and surveillance is poorly developed. There are numerous
reservoirs in nature, which are mainly mammals, birds and ticks. Domestic
ruminants are considered the main source of infection from which a large
number of different hosts can be infected. The serological studies we conducted
showed the presence of antibodies to C. burnetii in the Belgrade epizootic area.
Seroprevalence in cattle on intensive farms was 18%, while in family farms it
was 1.5%. In cows that aborted on farms, the prevalence was 49%, while in
family farms it was 1.9%. Infection in domestic animals is usually asymptomatic
and usually noticed only when reproductive disorders and abortions occur.
During abortion and labor, infected animals excrete large amount of bacteria
into the external environment, up to 1 billion per gram of placenta. Epidemics
in humans are most often associated with slaughterhouses and farms with
intensive breeding of sheep and goats. Clinically, Q fever is often asymptomatic
in humans, but also occures in the form of non-specific fever, atypical
pneumonia, hepatitis and endocarditis. Since the clinical signs of the disease
are nonspecific, laboratory evidence of infection is necessary to establish an
accurate diagnosis and initiate specific therapy. The disease is treated with the
use of specific antibiotics, and in untreated patients the mortality rate can be
high. Q fever is mainly transmitted by inhalation of contaminated aerosols,
contact with infected animals and contaminated environment, or by consumption
of infected milk and animal products. Prevention is directed to avoid
exposure of people, especially persons at risk, to animals and the polluted environment.
Good process management on farms lowers the level of environmental
contamination and prevents people and animals from coming into contact with
sources of infection. Improvements of the monitoring and reporting of infections
in animals enable the timely response of veterinary and health services, as
integral parts of one health system, thus achieving greater efficiency in controlling
and preventing the occurrence of Q fever and allowing the timely
treatment.",
publisher = "Beograd : Fakultet veterinarske medicine",
journal = "Četvrti regionalni simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 29. jun - 1. jul 2023",
title = "Epizootiologija Q groznice i njen društveno ekonomski uticaj i implikacije na javno zdravlje, Epizootiology of Q fever, its socioeconomic impact, and public health implications",
pages = "206-191"
}

Stanojević, S., Vojinović, D., Zdravković, N., Milovanović, B.,& Žutić, J.. (2023). Epizootiologija Q groznice i njen društveno ekonomski uticaj i implikacije na javno zdravlje. in Četvrti regionalni simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 29. jun - 1. jul 2023
Beograd : Fakultet veterinarske medicine., 191-206.

Stanojević S, Vojinović D, Zdravković N, Milovanović B, Žutić J. Epizootiologija Q groznice i njen društveno ekonomski uticaj i implikacije na javno zdravlje. in Četvrti regionalni simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 29. jun - 1. jul 2023. 2023;:191-206..

Stanojević, Slobodan, Vojinović, Dragica, Zdravković, Nemanja, Milovanović, Bojan, Žutić, Jadranka, "Epizootiologija Q groznice i njen društveno ekonomski uticaj i implikacije na javno zdravlje" in Četvrti regionalni simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 29. jun - 1. jul 2023 (2023):191-206.

TY  - JOUR
AU  - Glišić, Dimitrije
AU  - Zurovac Sapundžić, Zorana
AU  - Ninković, Milan
AU  - Milovanović, Bojan
AU  - Stevanović, Oliver
AU  - Laušević, Dejan
AU  - Đorđević, Milutin
AU  - Kureljušić, Branislav
AU  - Milićević, Vesna
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/724
AB  - This study aimed to describe the clinical course of SA-MCF and the duration of 
viremia in surviving cattle and to perform the phylogenetic analysis of the tegument 
protein gene of OvHV-2 in cattle in the Central Balkan. A farm housing two heifers 
and 7 sheep with a confirmed SA-MCF case were selected for the investigation. For 
the estimation of the length of viremia and the virus shedding, the animals were 
sampled repeatedly, weekly for two months. For the phylogenetic analysis, a 
retrospective study was performed on 21 samples from cattle, and 7 samples from 
sheep, from the Central Balkan. In the blood samples of the survived heifer, the 
OvHV-2 genome was detected until week 7, in corneal swabs, the OvHV-2 genome 
was detected until week 6, and in nasal swabs until week two of the study. A 
retrospective study revealed that out of 21 tested cattle, OvHV–2 was detected in 15 
(71.4%), and out of 7 tested sheep, three (42.9%) were positive. The sequenced 
samples show the highest percentage of similarity with the strains from Brasil 
KJ658293.1 (100%) and Germany HM216475.1 (100%).. Since there is a variety of 
different clinical signs similar to other notifiable diseases such as BVD, IBR, and 
FMD, there is a clear benefit in including SA-MCF in the differential diagnosis in 
cattle. As mixed farming in the Central Balkan is practiced, implementing SA-MCF 
monitoring in passive surveillance would allow a better understanding of the 
disease, ascertaining its prevalence and could provide new information regarding 
SA-MCF epidemiology.
PB  - Faislabad : Faculty of Veterinary Science, University of Agriculture
T2  - Pakistan Veterinary Journal
T1  - Detection and phylogenetic analysis of the Tegument protein gene of malignant catarrhal fever virus from clinical cases of cattle and sheep in the Central Balkan region
EP  - 365
IS  - 2
SP  - 361
VL  - 43
DO  - 10.29261/pakvetj/2023.032
ER  - 

@article{
author = "Glišić, Dimitrije and Zurovac Sapundžić, Zorana and Ninković, Milan and Milovanović, Bojan and Stevanović, Oliver and Laušević, Dejan and Đorđević, Milutin and Kureljušić, Branislav and Milićević, Vesna",
year = "2023",
abstract = "This study aimed to describe the clinical course of SA-MCF and the duration of 
viremia in surviving cattle and to perform the phylogenetic analysis of the tegument 
protein gene of OvHV-2 in cattle in the Central Balkan. A farm housing two heifers 
and 7 sheep with a confirmed SA-MCF case were selected for the investigation. For 
the estimation of the length of viremia and the virus shedding, the animals were 
sampled repeatedly, weekly for two months. For the phylogenetic analysis, a 
retrospective study was performed on 21 samples from cattle, and 7 samples from 
sheep, from the Central Balkan. In the blood samples of the survived heifer, the 
OvHV-2 genome was detected until week 7, in corneal swabs, the OvHV-2 genome 
was detected until week 6, and in nasal swabs until week two of the study. A 
retrospective study revealed that out of 21 tested cattle, OvHV–2 was detected in 15 
(71.4%), and out of 7 tested sheep, three (42.9%) were positive. The sequenced 
samples show the highest percentage of similarity with the strains from Brasil 
KJ658293.1 (100%) and Germany HM216475.1 (100%).. Since there is a variety of 
different clinical signs similar to other notifiable diseases such as BVD, IBR, and 
FMD, there is a clear benefit in including SA-MCF in the differential diagnosis in 
cattle. As mixed farming in the Central Balkan is practiced, implementing SA-MCF 
monitoring in passive surveillance would allow a better understanding of the 
disease, ascertaining its prevalence and could provide new information regarding 
SA-MCF epidemiology.",
publisher = "Faislabad : Faculty of Veterinary Science, University of Agriculture",
journal = "Pakistan Veterinary Journal",
title = "Detection and phylogenetic analysis of the Tegument protein gene of malignant catarrhal fever virus from clinical cases of cattle and sheep in the Central Balkan region",
pages = "365-361",
number = "2",
volume = "43",
doi = "10.29261/pakvetj/2023.032"
}

Glišić, D., Zurovac Sapundžić, Z., Ninković, M., Milovanović, B., Stevanović, O., Laušević, D., Đorđević, M., Kureljušić, B.,& Milićević, V.. (2023). Detection and phylogenetic analysis of the Tegument protein gene of malignant catarrhal fever virus from clinical cases of cattle and sheep in the Central Balkan region. in Pakistan Veterinary Journal
Faislabad : Faculty of Veterinary Science, University of Agriculture., 43(2), 361-365.
https://doi.org/10.29261/pakvetj/2023.032

Glišić D, Zurovac Sapundžić Z, Ninković M, Milovanović B, Stevanović O, Laušević D, Đorđević M, Kureljušić B, Milićević V. Detection and phylogenetic analysis of the Tegument protein gene of malignant catarrhal fever virus from clinical cases of cattle and sheep in the Central Balkan region. in Pakistan Veterinary Journal. 2023;43(2):361-365.
doi:10.29261/pakvetj/2023.032 .

Glišić, Dimitrije, Zurovac Sapundžić, Zorana, Ninković, Milan, Milovanović, Bojan, Stevanović, Oliver, Laušević, Dejan, Đorđević, Milutin, Kureljušić, Branislav, Milićević, Vesna, "Detection and phylogenetic analysis of the Tegument protein gene of malignant catarrhal fever virus from clinical cases of cattle and sheep in the Central Balkan region" in Pakistan Veterinary Journal, 43, no. 2 (2023):361-365,
https://doi.org/10.29261/pakvetj/2023.032 . .

TY  - JOUR
AU  - Milovanović, Bojan
AU  - Kureljušić, Branislav
AU  - Milićević, Vesna
AU  - Zdravković, Nemanja
AU  - Kureljušić, Jasna
AU  - Marinković, Darko
AU  - Maletić, Milan
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/694
AB  - Mycoplasma spp. is the cause of serious cattle health disorders that lead to poor
reproductive efficiency. Chronic seminal vesiculitis, infertility, vulvovaginitis and
dystocia were observed in Mycoplasma spp. infection. The objectives of the present study
were to investigate the frequency of Mycoplasmas in the uterus of postpartum dairy
cows and its potential role in the occurrence of subclinical endometritis. Our study
included 102 Holstein Friesian dairy cows 22-32 days postpartum. Uterine samples for
cytological, bacteriological, and molecular analysis were provided using Cytoprint AI®.
Metricheck® was used for the assessment of cervicovaginal mucus. After detection
of Mycoplasma spp. genome in uterine samples, the swabs of the vulva, vagina, nasal
swab, and samples of milk were sampled in order to exclude possible contamination
with other microorganisms as potential trigger of subclinical endometritis. The genome
of Mycoplasma spp. in the uterus was confirmed in 4 cows (2.40%). Sequencing of
the 16S RNA revealed that detected mycoplasmas belonged to Mycoplasma bovigenitalium
and Mycoplasma tauri species, with a prevalence of 1.80% and 0.6%, respectively. The
cytological evaluation showed a 69.90%, 54.87% and 48.33% of polymorphonuclear cells
for M. bovigenitalium positive cows, and 61.64% for M. tauri positive cow. Bacteriological
examination revealed Trueperella pyogenes in uterine samples in all 4 observed cows. This
is the first study where M. tauri was detected in the uterus of a live cow with subclinical enodmetritis. In this paper we hypothesized that M. bovigenitalium and M. tauri may have
a certain role in the etiology of subclinical enodmetritis.
AB  - Mycoplasma spp. prouzrokuju oboljenja goveda koja za posledicu imaju smanjenje
reproduktivne efikasnosti muznih krava. Mycoplasma spp. kod goveda dovode do
hroničnog seminalnog vezikulitisa, vulvovaginitisa, distokije i neplodnosti. Cilj ove
studije je bio da se ispita učestalost mikoplazmi u materici kod krava nakon teljenja i
utvrdi da li postoji potencijalna veza sa nastankom subkliničkog endometritisa. Studija
je obuhvatila 102 muzne krave Holštajn Frizijske rase od 22-32. dana nakon teljenja.
Brisevi endometrijuma za citološku, bakteriološku i molekularnu analizu su dobijeni
korišćenjem Cytoprint AI®. Metricheck® je korišćen za procenu kvaliteta cervikovaginalne
sluzi. Nakon detekcije genoma Mycoplasma spp. u brisevima endometrijuma,
uzorkovani su brisevi vulve, vagine, nosa i uzorci mleka kako bi se isključila moguća
kontaminacija drugim mikroorganizmima kao potencijalnim prouzrokovačima
subkliničkog endometritisa. Genom Mycoplasma spp. u brisu endometrijuma je utvrđen
kod 4 krave (2,40%). Sekvenciranjem 16S RNK utvrđeno je da detektovane mikoplazme
pripadaju Mycoplasma bovigenitalium i Mycoplasma tauri vrstama, sa prevalencijom
od 1,80%, odnosno 0,6%. Citološkom analizom je utvrđen procenat polimorfonuklearnih
ćelija od 69,90%, 54,87% i 48,33% kod krava pozitivnih na M. bovigenitalium i
61,64% kod krave pozitivne na M. tauri. Bakteriološkom analizom je utvrđena Trueperella
pyogenes u uzorcima endometrijuma kod sve 4 ispitane krave. Ovo je prva studija u
kojoj je M. tauri otkrivena u brisu iz materice žive krave sa subkliničkim endometritisom.
U ovom radu, pretpostavljamo da M. bovigenitalium i M. tauri mogu imati izvesnu
ulogu u etiologiji subkliničkog endometritisa.
PB  - Beograd : Fakultet veterinarske medicine
T2  - Acta Veterinaria-Beograd
T1  - Detection of Mycoplasma bovigenitalium and Mycoplasma tauri in holstein friesian dairy cows with subclinical endometritis
T1  - Detekcija genoma Mycoplasma bovigenitalium i Mycoplasma tauri kod mlečnih krava holštajnfrizijske rase sa supkliničkim endometritisom
EP  - 431
IS  - 3
SP  - 421
VL  - 73
DO  - 10.2478/acve-2023-0031
ER  - 

@article{
author = "Milovanović, Bojan and Kureljušić, Branislav and Milićević, Vesna and Zdravković, Nemanja and Kureljušić, Jasna and Marinković, Darko and Maletić, Milan",
year = "2023",
abstract = "Mycoplasma spp. is the cause of serious cattle health disorders that lead to poor
reproductive efficiency. Chronic seminal vesiculitis, infertility, vulvovaginitis and
dystocia were observed in Mycoplasma spp. infection. The objectives of the present study
were to investigate the frequency of Mycoplasmas in the uterus of postpartum dairy
cows and its potential role in the occurrence of subclinical endometritis. Our study
included 102 Holstein Friesian dairy cows 22-32 days postpartum. Uterine samples for
cytological, bacteriological, and molecular analysis were provided using Cytoprint AI®.
Metricheck® was used for the assessment of cervicovaginal mucus. After detection
of Mycoplasma spp. genome in uterine samples, the swabs of the vulva, vagina, nasal
swab, and samples of milk were sampled in order to exclude possible contamination
with other microorganisms as potential trigger of subclinical endometritis. The genome
of Mycoplasma spp. in the uterus was confirmed in 4 cows (2.40%). Sequencing of
the 16S RNA revealed that detected mycoplasmas belonged to Mycoplasma bovigenitalium
and Mycoplasma tauri species, with a prevalence of 1.80% and 0.6%, respectively. The
cytological evaluation showed a 69.90%, 54.87% and 48.33% of polymorphonuclear cells
for M. bovigenitalium positive cows, and 61.64% for M. tauri positive cow. Bacteriological
examination revealed Trueperella pyogenes in uterine samples in all 4 observed cows. This
is the first study where M. tauri was detected in the uterus of a live cow with subclinical enodmetritis. In this paper we hypothesized that M. bovigenitalium and M. tauri may have
a certain role in the etiology of subclinical enodmetritis., Mycoplasma spp. prouzrokuju oboljenja goveda koja za posledicu imaju smanjenje
reproduktivne efikasnosti muznih krava. Mycoplasma spp. kod goveda dovode do
hroničnog seminalnog vezikulitisa, vulvovaginitisa, distokije i neplodnosti. Cilj ove
studije je bio da se ispita učestalost mikoplazmi u materici kod krava nakon teljenja i
utvrdi da li postoji potencijalna veza sa nastankom subkliničkog endometritisa. Studija
je obuhvatila 102 muzne krave Holštajn Frizijske rase od 22-32. dana nakon teljenja.
Brisevi endometrijuma za citološku, bakteriološku i molekularnu analizu su dobijeni
korišćenjem Cytoprint AI®. Metricheck® je korišćen za procenu kvaliteta cervikovaginalne
sluzi. Nakon detekcije genoma Mycoplasma spp. u brisevima endometrijuma,
uzorkovani su brisevi vulve, vagine, nosa i uzorci mleka kako bi se isključila moguća
kontaminacija drugim mikroorganizmima kao potencijalnim prouzrokovačima
subkliničkog endometritisa. Genom Mycoplasma spp. u brisu endometrijuma je utvrđen
kod 4 krave (2,40%). Sekvenciranjem 16S RNK utvrđeno je da detektovane mikoplazme
pripadaju Mycoplasma bovigenitalium i Mycoplasma tauri vrstama, sa prevalencijom
od 1,80%, odnosno 0,6%. Citološkom analizom je utvrđen procenat polimorfonuklearnih
ćelija od 69,90%, 54,87% i 48,33% kod krava pozitivnih na M. bovigenitalium i
61,64% kod krave pozitivne na M. tauri. Bakteriološkom analizom je utvrđena Trueperella
pyogenes u uzorcima endometrijuma kod sve 4 ispitane krave. Ovo je prva studija u
kojoj je M. tauri otkrivena u brisu iz materice žive krave sa subkliničkim endometritisom.
U ovom radu, pretpostavljamo da M. bovigenitalium i M. tauri mogu imati izvesnu
ulogu u etiologiji subkliničkog endometritisa.",
publisher = "Beograd : Fakultet veterinarske medicine",
journal = "Acta Veterinaria-Beograd",
title = "Detection of Mycoplasma bovigenitalium and Mycoplasma tauri in holstein friesian dairy cows with subclinical endometritis, Detekcija genoma Mycoplasma bovigenitalium i Mycoplasma tauri kod mlečnih krava holštajnfrizijske rase sa supkliničkim endometritisom",
pages = "431-421",
number = "3",
volume = "73",
doi = "10.2478/acve-2023-0031"
}

Milovanović, B., Kureljušić, B., Milićević, V., Zdravković, N., Kureljušić, J., Marinković, D.,& Maletić, M.. (2023). Detection of Mycoplasma bovigenitalium and Mycoplasma tauri in holstein friesian dairy cows with subclinical endometritis. in Acta Veterinaria-Beograd
Beograd : Fakultet veterinarske medicine., 73(3), 421-431.
https://doi.org/10.2478/acve-2023-0031

Milovanović B, Kureljušić B, Milićević V, Zdravković N, Kureljušić J, Marinković D, Maletić M. Detection of Mycoplasma bovigenitalium and Mycoplasma tauri in holstein friesian dairy cows with subclinical endometritis. in Acta Veterinaria-Beograd. 2023;73(3):421-431.
doi:10.2478/acve-2023-0031 .

Milovanović, Bojan, Kureljušić, Branislav, Milićević, Vesna, Zdravković, Nemanja, Kureljušić, Jasna, Marinković, Darko, Maletić, Milan, "Detection of Mycoplasma bovigenitalium and Mycoplasma tauri in holstein friesian dairy cows with subclinical endometritis" in Acta Veterinaria-Beograd, 73, no. 3 (2023):421-431,
https://doi.org/10.2478/acve-2023-0031 . .

TY  - CONF
AU  - Kureljušić, Branislav
AU  - Savić, Božidar
AU  - Milovanović, Bojan
AU  - Maletić, Jelena
AU  - Ninković, Milan
AU  - Jezdimirović, Nemanja
AU  - Prodanov Radulović, Jasna
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/686
AB  - Biosecurity measures together with the efficient on-farm management embraces all aspects of
the prevention of pathogens entering pig farms, regardless of structure and animal number. In
the present study assessment of biosecurity measures in the commercial (industrial) pig farms in
Serbia was carried out through application of the questionnaire to farm veterinarians.
To assess biosecurity in commercial (industrial) farms, a Biocheck online survey
(https://biocheckgent.com) was conducted in the ten commercial pigs farms located in the
Northern part of Serbia where industrial pig production is localized and the highest density of pig
production can be found.
The results of the external biosecurity assessment showed a mean score of 75.55%, compared to
the average in Serbia, which was 68.55%, and the global average of 73%. External biosecurity
assessment showed the lowest scores for feed, water and equipment supply (47%). The
assessment of internal biosecurity revealed a score of 54.89% compared to the national average
of 44.22% and the world average of 64.11%. Regarding internal biosecurity, the lowest
percentage score (37.22%) was confirmed for farrowing and suckling period and for finishing unit
(37.67%). The overall biosecurity score for commercial pig farms included in this study was
65.55%. The national average was 56.33%, while the global average was 68.67%.
This study showed a bit less lower level of biosecurity measures in commercial pig farms in Serbia
comparing with global average. Certainly, having in mind the different pig production systems in
Serbia, there is urgent need to improve biosecurity in industrial farms. Serbia as a Western Balkan
country, has variable pig farming system characterized by a domination of traditional family
farms, smallholdings, backyard production and semi-free and free-range. Currently, traditional
farming methods in Serbia represent a cultural identity. The biosecurity measures that could be
implemented in extisted animal production systems need to be urgently identified.
PB  - Ghent : Faculty of veterinary medicine
C3  - COST action CA20103 "Biosecurity Enhanced Through Training Evaluation and Raising Awareness"
T1  - Assessment of biosecurity measures in the commercial (industrial) pig farms in Serbia
EP  - 39
SP  - 39
ER  - 

@conference{
author = "Kureljušić, Branislav and Savić, Božidar and Milovanović, Bojan and Maletić, Jelena and Ninković, Milan and Jezdimirović, Nemanja and Prodanov Radulović, Jasna",
year = "2023",
abstract = "Biosecurity measures together with the efficient on-farm management embraces all aspects of
the prevention of pathogens entering pig farms, regardless of structure and animal number. In
the present study assessment of biosecurity measures in the commercial (industrial) pig farms in
Serbia was carried out through application of the questionnaire to farm veterinarians.
To assess biosecurity in commercial (industrial) farms, a Biocheck online survey
(https://biocheckgent.com) was conducted in the ten commercial pigs farms located in the
Northern part of Serbia where industrial pig production is localized and the highest density of pig
production can be found.
The results of the external biosecurity assessment showed a mean score of 75.55%, compared to
the average in Serbia, which was 68.55%, and the global average of 73%. External biosecurity
assessment showed the lowest scores for feed, water and equipment supply (47%). The
assessment of internal biosecurity revealed a score of 54.89% compared to the national average
of 44.22% and the world average of 64.11%. Regarding internal biosecurity, the lowest
percentage score (37.22%) was confirmed for farrowing and suckling period and for finishing unit
(37.67%). The overall biosecurity score for commercial pig farms included in this study was
65.55%. The national average was 56.33%, while the global average was 68.67%.
This study showed a bit less lower level of biosecurity measures in commercial pig farms in Serbia
comparing with global average. Certainly, having in mind the different pig production systems in
Serbia, there is urgent need to improve biosecurity in industrial farms. Serbia as a Western Balkan
country, has variable pig farming system characterized by a domination of traditional family
farms, smallholdings, backyard production and semi-free and free-range. Currently, traditional
farming methods in Serbia represent a cultural identity. The biosecurity measures that could be
implemented in extisted animal production systems need to be urgently identified.",
publisher = "Ghent : Faculty of veterinary medicine",
journal = "COST action CA20103 "Biosecurity Enhanced Through Training Evaluation and Raising Awareness"",
title = "Assessment of biosecurity measures in the commercial (industrial) pig farms in Serbia",
pages = "39-39"
}

Kureljušić, B., Savić, B., Milovanović, B., Maletić, J., Ninković, M., Jezdimirović, N.,& Prodanov Radulović, J.. (2023). Assessment of biosecurity measures in the commercial (industrial) pig farms in Serbia. in COST action CA20103 "Biosecurity Enhanced Through Training Evaluation and Raising Awareness"
Ghent : Faculty of veterinary medicine., 39-39.

Kureljušić B, Savić B, Milovanović B, Maletić J, Ninković M, Jezdimirović N, Prodanov Radulović J. Assessment of biosecurity measures in the commercial (industrial) pig farms in Serbia. in COST action CA20103 "Biosecurity Enhanced Through Training Evaluation and Raising Awareness". 2023;:39-39..

Kureljušić, Branislav, Savić, Božidar, Milovanović, Bojan, Maletić, Jelena, Ninković, Milan, Jezdimirović, Nemanja, Prodanov Radulović, Jasna, "Assessment of biosecurity measures in the commercial (industrial) pig farms in Serbia" in COST action CA20103 "Biosecurity Enhanced Through Training Evaluation and Raising Awareness" (2023):39-39.

TY  - CONF
AU  - Maksimović Zorić, Jelena
AU  - Veljović, Ljubiša
AU  - Glišić, Dimitrije
AU  - Jezdimirović, Nemanja
AU  - Savić, Božidar
AU  - Milovanović, Bojan
AU  - Maletić, Jelena
AU  - Radosavljević, Vladimir
AU  - Kureljušić, Branislav
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/652
AB  - Swine influenza is a worldwide present respiratory disease of swine, in majority of cases caused by H1N1,
H1N2, and H3N2 subtypes of swine influenza A virus (swIAV). Exploration of the swIAV in Serbia in recent
ten years mainly was oriented to the passive surveillance and examination within PRDC on commercial pig
farms. The latest results suggest circulation of the viruses in the majority of the analysed commercial farms
and the presence of two subtypes - H1N1 and H3N2. Even though the problem exists none of the farms
apply vaccination as a measure of disease control. The aim of this research is to analyse the results of
passive surveillance on the two commercial farms conducted from 2020th to 2022nd.
A total of 94 samples for molecular analyses and 528 samples for serological analyses were submitted to
the Institute of Veterinary Medicine of Serbia during this period. These samples were obtained from swine
raised in two commercial farms (farm A and farm B) that practice farrow-to-finish production and have
implemented satisfactory biosecurity measures. The samples for molecular examination were mummified
fetuses, nasal swabs, oral fluids, parenchymatous organs, lungs, and sera. All samples were tested by M
gene real-time RT-PCR, according to the accredited WHO protocol (2009) for detection of influenza A
viruses. One virus was typed using multiplex real-time RT-PCR protocols described by Henritzi et al. (2016).
Serological examination was conducted using ELISA test (Ingezim Influenza A Kit, Ingenasa), and tested sera
originated from sows, suckling piglets, and nursing pigs.
During three year period influenza A viruses were detected on both farms, having on average 55,3% of the
analyzed samples positive. The highest detection rate was amongst the nasal swab (78.7%) and oral fluid
samples (83.3%), while virus was not confirmed in mummified fetuses and sera. Most of the samples
analyzed by PCR originated from farm A (80 samples), and the majority of tested were positive (60%).
Molecular typing revealed presence of H1N1 subtype in farm A that is in accordance with previous results
that confirmed H1N1 as dominating subtype that circulates in commercial farms in Serbia. Serology
examination confirmed a high overall seroprevalence of 64,6%. The serum samples from nursing pigs
accounted for the highest proportion (95.4%) among those examined, as respiratory issues linked to PRDC
typically manifest in this group. Given the endemic presence of swIAVs in the farms under analysis, there is
a pressing need to conduct further research on the circulating viruses and enhance the control measures
employed on these farms.
PB  - ESFLU
C3  - ESFLU Scientific meeting, Barcelona, 25-27th April 2023
T1  - Serological and molecular investigation of influenza in commercial pig farms in Serbia
ER  - 

@conference{
author = "Maksimović Zorić, Jelena and Veljović, Ljubiša and Glišić, Dimitrije and Jezdimirović, Nemanja and Savić, Božidar and Milovanović, Bojan and Maletić, Jelena and Radosavljević, Vladimir and Kureljušić, Branislav",
year = "2023",
abstract = "Swine influenza is a worldwide present respiratory disease of swine, in majority of cases caused by H1N1,
H1N2, and H3N2 subtypes of swine influenza A virus (swIAV). Exploration of the swIAV in Serbia in recent
ten years mainly was oriented to the passive surveillance and examination within PRDC on commercial pig
farms. The latest results suggest circulation of the viruses in the majority of the analysed commercial farms
and the presence of two subtypes - H1N1 and H3N2. Even though the problem exists none of the farms
apply vaccination as a measure of disease control. The aim of this research is to analyse the results of
passive surveillance on the two commercial farms conducted from 2020th to 2022nd.
A total of 94 samples for molecular analyses and 528 samples for serological analyses were submitted to
the Institute of Veterinary Medicine of Serbia during this period. These samples were obtained from swine
raised in two commercial farms (farm A and farm B) that practice farrow-to-finish production and have
implemented satisfactory biosecurity measures. The samples for molecular examination were mummified
fetuses, nasal swabs, oral fluids, parenchymatous organs, lungs, and sera. All samples were tested by M
gene real-time RT-PCR, according to the accredited WHO protocol (2009) for detection of influenza A
viruses. One virus was typed using multiplex real-time RT-PCR protocols described by Henritzi et al. (2016).
Serological examination was conducted using ELISA test (Ingezim Influenza A Kit, Ingenasa), and tested sera
originated from sows, suckling piglets, and nursing pigs.
During three year period influenza A viruses were detected on both farms, having on average 55,3% of the
analyzed samples positive. The highest detection rate was amongst the nasal swab (78.7%) and oral fluid
samples (83.3%), while virus was not confirmed in mummified fetuses and sera. Most of the samples
analyzed by PCR originated from farm A (80 samples), and the majority of tested were positive (60%).
Molecular typing revealed presence of H1N1 subtype in farm A that is in accordance with previous results
that confirmed H1N1 as dominating subtype that circulates in commercial farms in Serbia. Serology
examination confirmed a high overall seroprevalence of 64,6%. The serum samples from nursing pigs
accounted for the highest proportion (95.4%) among those examined, as respiratory issues linked to PRDC
typically manifest in this group. Given the endemic presence of swIAVs in the farms under analysis, there is
a pressing need to conduct further research on the circulating viruses and enhance the control measures
employed on these farms.",
publisher = "ESFLU",
journal = "ESFLU Scientific meeting, Barcelona, 25-27th April 2023",
title = "Serological and molecular investigation of influenza in commercial pig farms in Serbia"
}

Maksimović Zorić, J., Veljović, L., Glišić, D., Jezdimirović, N., Savić, B., Milovanović, B., Maletić, J., Radosavljević, V.,& Kureljušić, B.. (2023). Serological and molecular investigation of influenza in commercial pig farms in Serbia. in ESFLU Scientific meeting, Barcelona, 25-27th April 2023
ESFLU..

Maksimović Zorić J, Veljović L, Glišić D, Jezdimirović N, Savić B, Milovanović B, Maletić J, Radosavljević V, Kureljušić B. Serological and molecular investigation of influenza in commercial pig farms in Serbia. in ESFLU Scientific meeting, Barcelona, 25-27th April 2023. 2023;..

Maksimović Zorić, Jelena, Veljović, Ljubiša, Glišić, Dimitrije, Jezdimirović, Nemanja, Savić, Božidar, Milovanović, Bojan, Maletić, Jelena, Radosavljević, Vladimir, Kureljušić, Branislav, "Serological and molecular investigation of influenza in commercial pig farms in Serbia" in ESFLU Scientific meeting, Barcelona, 25-27th April 2023 (2023).

TY  - CONF
AU  - Kureljušić, Branislav
AU  - Savić, Božidar
AU  - Milićević, Vesna
AU  - Radanović, Oliver
AU  - Jezdimirović, Nemanja
AU  - Milovanović, Bojan
AU  - Weissenbacher-Lang, Christiane
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/544
AB  - U etiopatogenezi nekroze ušne školjke kod svinja mogu biti uključeni
infektivni agensi kao i neinfektivni faktori. Griža ušiju kao oblik
kanibalizma obično predstavlja uzrok nekroze ušne školjke kod svinja, ali se
nekroza može pojaviti i bez ikakvih dokaza o postojanju griže ušiju. Iako su
istraživači ispitivali ulogu različitih uzročnika za razvoj lezija ušne
školjke, do sada nije potvrđena definitivna etiologija.
U ovom radu opisan je slučaj nekroze ušne školjke 10 uginulih svinja sa jedne
komercijalne farme svinja u Srbiji. Prema podacima ordinirajućeg veterinara
farme, lezije na ušnoj školjki su prvi put otkrivene kod svinja starosti između
45 i 50 dana kod više od 80% jedinki, u svim boksevima u odgajivalištu. Za
ispitivanje su uzete ušne školjke uginulih svinja i sprovedena su
patomorfološka, bakteriološka i molekularno-genetička ispitivanja.
Makroskopski, ustanovljene lezije su opisane kao blagi, superficijalni
dermatitis, a u težim slučajevima, bila je prisutna duboka inflamacija dermisa
sa ulceracijom i nekrozom. Histopatološkim pregledom utvrđen je erozivni i
ulcerozni dermatitis ušne školjke sa infiltracijom neutrofilnim granulocitima
i limfocitima, fibroplazija i bazofilne kolonije bakterija u krastama.
U osam uzoraka krasti i tkiva ušiju izolovani su S. aureus (MRSA soj), S. hyicus i
Streptococcus grupe C. U dva uzorka otkriven je samo S. hyicus. Svi uzorci bili su
pozitivni na T. denticola/T. putidum grupu i negativni na Mycoplasma suis. Sedam
od deset oralnih briseva bilo je pozitivno na T. denticola/T. putidum grupu. Dva
PCR proizvoda su sekvencionirana i filogenetska analiza je pokazala genetsku
sličnost sekvenci nukleotida sa T. medium/T. vincentii grupom i Treponema pedis.
Iako su bakterijski agensi uspešno utvrđeni u ispitanim uzorcima ušnih
školjki, neinfektivni faktori, uključujući dokazanu visoku vlažnost vazduha i
deficit sirovih proteina u obroku, takođe mogu biti uključeni u razvoj sindroma
nekroze ušne školjke. Istovremena detekcija treponema u uzorcima krasti i tkiva
ušiju i uzorcima brisa usne šupljine može ukazivati na prenos bakterija tokom
griže ušiju. Ovaj slučaj još jednom naglašava multifaktorijalni karakter ovog
sindroma što otežava kontrolu i prevenciju ove bolesti.
AB  - Infectious agents as well as non-infectious factors can be involved in the
etiopathogenesis of porcine ear necrosis. Furthermore, ear biting is commonly
suggested as a cause of ear necrosis in pigs, but it may also appear without any
evidence of ear biting. Although many causative agents for the development of ear
lesions have been suggested by investigators no definitive aetiology has been
identified so far.
This report describes case of ear necrosis of 10 dead pigs from one commercial pig
farm in Serbia. According to to the data obtained by the farm veterinarian, ear lesions
were first detected in pigs between 45 and 50 days of age from all pens and more than
80% of the pig population was affected. Auriculae of dead pigs were sampled and
pathomorphological, bacteriological and molecular-genetic examinations were carried
out.
The lesions ranged from mild, superficial dermatitis to severe, deep inflammation with
exudation, ulceration and necrosis. Histopathological examination revealed erosive
and ulcerative dermatitis of the pinna with neutrophilic and lymphocytic infiltration,
fibroplasia and bacterial colonies in the crusts.
In eight ear tissue scraping samples, S. aureus (MRSA strain), S. hyicus and
Streptococcus group C were isolated. In two samples only S. hyicus was detected. All
ear tissue scraping samples tested positive for the T. denticola/T. putidum group and
negative for Mycoplasma suis. Seven out of ten oral swabs were positive for the T.
denticola/T. putidum group. Two of the PCR products were sequenced, and the
phylogenetic analysis showed a genetic relationship of the treponeme nucleotide
sequences to the T. medium/T. vincentii group and Treponema pedis.
Although bacterial agents were determined successfully in ear tissue scraping
samples, non-infectious triggering factors including proven high humidity levels and
crude protein deficiencies may also have been involved in the development of ear
necrosis syndrome. The concurrent detection of Treponema in both ear tissue
scrapings and oral swab samples might indicate the transmission of the bacterium
during ear biting. The present case once again highlights the multifactorial character
of this condition that renders the control and prevention of this disease difficult.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani)
T1  - Nekroza ušne školjke kod svinja – ispitivanje moguće infektivne etiologije
T1  - Porcine ear necrosis – investigation of possible infectious etiology
EP  - 143
SP  - 142
ER  - 

@conference{
author = "Kureljušić, Branislav and Savić, Božidar and Milićević, Vesna and Radanović, Oliver and Jezdimirović, Nemanja and Milovanović, Bojan and Weissenbacher-Lang, Christiane",
year = "2023",
abstract = "U etiopatogenezi nekroze ušne školjke kod svinja mogu biti uključeni
infektivni agensi kao i neinfektivni faktori. Griža ušiju kao oblik
kanibalizma obično predstavlja uzrok nekroze ušne školjke kod svinja, ali se
nekroza može pojaviti i bez ikakvih dokaza o postojanju griže ušiju. Iako su
istraživači ispitivali ulogu različitih uzročnika za razvoj lezija ušne
školjke, do sada nije potvrđena definitivna etiologija.
U ovom radu opisan je slučaj nekroze ušne školjke 10 uginulih svinja sa jedne
komercijalne farme svinja u Srbiji. Prema podacima ordinirajućeg veterinara
farme, lezije na ušnoj školjki su prvi put otkrivene kod svinja starosti između
45 i 50 dana kod više od 80% jedinki, u svim boksevima u odgajivalištu. Za
ispitivanje su uzete ušne školjke uginulih svinja i sprovedena su
patomorfološka, bakteriološka i molekularno-genetička ispitivanja.
Makroskopski, ustanovljene lezije su opisane kao blagi, superficijalni
dermatitis, a u težim slučajevima, bila je prisutna duboka inflamacija dermisa
sa ulceracijom i nekrozom. Histopatološkim pregledom utvrđen je erozivni i
ulcerozni dermatitis ušne školjke sa infiltracijom neutrofilnim granulocitima
i limfocitima, fibroplazija i bazofilne kolonije bakterija u krastama.
U osam uzoraka krasti i tkiva ušiju izolovani su S. aureus (MRSA soj), S. hyicus i
Streptococcus grupe C. U dva uzorka otkriven je samo S. hyicus. Svi uzorci bili su
pozitivni na T. denticola/T. putidum grupu i negativni na Mycoplasma suis. Sedam
od deset oralnih briseva bilo je pozitivno na T. denticola/T. putidum grupu. Dva
PCR proizvoda su sekvencionirana i filogenetska analiza je pokazala genetsku
sličnost sekvenci nukleotida sa T. medium/T. vincentii grupom i Treponema pedis.
Iako su bakterijski agensi uspešno utvrđeni u ispitanim uzorcima ušnih
školjki, neinfektivni faktori, uključujući dokazanu visoku vlažnost vazduha i
deficit sirovih proteina u obroku, takođe mogu biti uključeni u razvoj sindroma
nekroze ušne školjke. Istovremena detekcija treponema u uzorcima krasti i tkiva
ušiju i uzorcima brisa usne šupljine može ukazivati na prenos bakterija tokom
griže ušiju. Ovaj slučaj još jednom naglašava multifaktorijalni karakter ovog
sindroma što otežava kontrolu i prevenciju ove bolesti., Infectious agents as well as non-infectious factors can be involved in the
etiopathogenesis of porcine ear necrosis. Furthermore, ear biting is commonly
suggested as a cause of ear necrosis in pigs, but it may also appear without any
evidence of ear biting. Although many causative agents for the development of ear
lesions have been suggested by investigators no definitive aetiology has been
identified so far.
This report describes case of ear necrosis of 10 dead pigs from one commercial pig
farm in Serbia. According to to the data obtained by the farm veterinarian, ear lesions
were first detected in pigs between 45 and 50 days of age from all pens and more than
80% of the pig population was affected. Auriculae of dead pigs were sampled and
pathomorphological, bacteriological and molecular-genetic examinations were carried
out.
The lesions ranged from mild, superficial dermatitis to severe, deep inflammation with
exudation, ulceration and necrosis. Histopathological examination revealed erosive
and ulcerative dermatitis of the pinna with neutrophilic and lymphocytic infiltration,
fibroplasia and bacterial colonies in the crusts.
In eight ear tissue scraping samples, S. aureus (MRSA strain), S. hyicus and
Streptococcus group C were isolated. In two samples only S. hyicus was detected. All
ear tissue scraping samples tested positive for the T. denticola/T. putidum group and
negative for Mycoplasma suis. Seven out of ten oral swabs were positive for the T.
denticola/T. putidum group. Two of the PCR products were sequenced, and the
phylogenetic analysis showed a genetic relationship of the treponeme nucleotide
sequences to the T. medium/T. vincentii group and Treponema pedis.
Although bacterial agents were determined successfully in ear tissue scraping
samples, non-infectious triggering factors including proven high humidity levels and
crude protein deficiencies may also have been involved in the development of ear
necrosis syndrome. The concurrent detection of Treponema in both ear tissue
scrapings and oral swab samples might indicate the transmission of the bacterium
during ear biting. The present case once again highlights the multifactorial character
of this condition that renders the control and prevention of this disease difficult.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani)",
title = "Nekroza ušne školjke kod svinja – ispitivanje moguće infektivne etiologije, Porcine ear necrosis – investigation of possible infectious etiology",
pages = "143-142"
}

Kureljušić, B., Savić, B., Milićević, V., Radanović, O., Jezdimirović, N., Milovanović, B.,& Weissenbacher-Lang, C.. (2023). Nekroza ušne školjke kod svinja – ispitivanje moguće infektivne etiologije. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani)
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 142-143.

Kureljušić B, Savić B, Milićević V, Radanović O, Jezdimirović N, Milovanović B, Weissenbacher-Lang C. Nekroza ušne školjke kod svinja – ispitivanje moguće infektivne etiologije. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani). 2023;:142-143..

Kureljušić, Branislav, Savić, Božidar, Milićević, Vesna, Radanović, Oliver, Jezdimirović, Nemanja, Milovanović, Bojan, Weissenbacher-Lang, Christiane, "Nekroza ušne školjke kod svinja – ispitivanje moguće infektivne etiologije" in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani) (2023):142-143.

TY  - CONF
AU  - Glišić, Dimitrije
AU  - Veljović, Ljubiša
AU  - Maletić, Jelena
AU  - Kureljušić, Branislav
AU  - Ninković, Milan
AU  - Milovanović, Bojan
AU  - Milićević, Vesna
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/540
AB  - Mnoge zarazne bolesti se prenose vazduhom. Jedinke koje pokazuju kliničke
simptome respiratornih bolesti izlučuju uzročnika u sekretu, zatim
iskašljavanjem u vidu kapljica uzročnici dospevaju u spoljašnju sredinu u
kojoj mogu opstati zaštićeni unutar kapljica. Crevni patogeni se takođe mogu
širiti putem vazduha uz pomoć čestica prašine koje se podižu aktivnim
kretanjem životinja i osoblja na farmi. Neinvazivne metode uzorkovanja su
vrlo značajne za brz i jednostavan uvid u zdravstveno stanje zapata,
isključujući individualne varijacije između jedinki, i ujedno neometajući
redovne aktivnosti na farmi. Ranije je pokazano da je oralna tečnost kao uzorak
dobijen neinvazivnim putem veoma pouzdan za utvrđivanje statusa zapata.
Međutim, u našoj studiji, ispitali smo prisustvo virusnih patogena svinja u
vazduhu, uključujući virus influence svinja, cirkovirus tip dva svinja kao i
virus respiratornog i reproduktivnog sindroma svinja. Vazduh je uzorkovan
upotrebom „Aerokolekt“ uređaja. Princip rada „Aerokolekt“ uređaja je
zasnovan na strujanju vazduha kroz jednokratan čip iz kog se u laboratoriji
direktnom elucijom dobija uzorak nukleinske kiseline pogodan za PCR
analize. Uzorci su sakupljeni sa 5 farmi svinja koje imaju potvrđeno prisustvo
navedenih virusnih patogena. Rezultati ispitivanja vazduha su poređeni sa
rezultatima dobijenim iz uobičajeno sakupljenih uzoraka. Preliminarnim
rezultatima je utvrđeno da je ispitivanje vazduha pogodno za utvrđivanje
prisustva navedenih uzročnika na farmi. Primenom metodologije uzorkovanja
vazduha, može se vršiti aktivan nadzor na prisustvo patogena, održavanje
slobodnog statusa od bolesti, kao i rana detekcija patogena. Značajne
prednosti ispitivanja vazduha su i očuvanje dobrobiti, usled izostanka
direktnog kontakta sa životinjama.
AB  - Many infectious diseases are transmitted by air. Individuals which show clinical signs
of respiratory diseases excrete the culprit, and through expectoration in the form of
droplets, the causative agents end up in the external environment where they can
remain be protected within the droplets. Enteral pathogens can also be transmitted by
air, through dust particles lifted by active animal and farm staff movement. Noninvasive sampling methods are significant for swift and simple insight into the health
status of a herd, excluding individual variations, and withal not disrupting regular
farm activities. Earlier it was shown that oral fluid samples, acquired by non-invasive
methods, are a reliable way of determining the status of a herd. Meanwhile, our study
tested the presence of viral swine pathogens in the air, including the swine influenza
virus, circovirus type two, and the respiratory and reproductive syndrome virus. The
air was sampled using the “Aerocollect” device. The working principle of the
“Aerocollect” apparatus is based on the flow of air through a single-use chip from
which nucleic acid can be eluted and used for PCR analysis. The samples were
collected from five pig farms, which had a confirmed presence of the beforementioned
viral swine pathogens. The results of the analysis were compared with results from
traditionally collected samples. The preliminary results confirm that air sampling can
be used for determining the presence of the mentioned pathogens on a farm. By
applying air sampling methodology, active surveillance for the presence of pathogens
can be conducted, as well as the maintenance of free disease status, and early pathogen
detection. Considerable advantages of air sampling are the preservation of animal
welfare and the absence of direct animal contact.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani)
T1  - Virusni patogeni svinja u vazduhu
T1  - Viral swine pathogens in the air
EP  - 85
SP  - 84
ER  - 

@conference{
author = "Glišić, Dimitrije and Veljović, Ljubiša and Maletić, Jelena and Kureljušić, Branislav and Ninković, Milan and Milovanović, Bojan and Milićević, Vesna",
year = "2023",
abstract = "Mnoge zarazne bolesti se prenose vazduhom. Jedinke koje pokazuju kliničke
simptome respiratornih bolesti izlučuju uzročnika u sekretu, zatim
iskašljavanjem u vidu kapljica uzročnici dospevaju u spoljašnju sredinu u
kojoj mogu opstati zaštićeni unutar kapljica. Crevni patogeni se takođe mogu
širiti putem vazduha uz pomoć čestica prašine koje se podižu aktivnim
kretanjem životinja i osoblja na farmi. Neinvazivne metode uzorkovanja su
vrlo značajne za brz i jednostavan uvid u zdravstveno stanje zapata,
isključujući individualne varijacije između jedinki, i ujedno neometajući
redovne aktivnosti na farmi. Ranije je pokazano da je oralna tečnost kao uzorak
dobijen neinvazivnim putem veoma pouzdan za utvrđivanje statusa zapata.
Međutim, u našoj studiji, ispitali smo prisustvo virusnih patogena svinja u
vazduhu, uključujući virus influence svinja, cirkovirus tip dva svinja kao i
virus respiratornog i reproduktivnog sindroma svinja. Vazduh je uzorkovan
upotrebom „Aerokolekt“ uređaja. Princip rada „Aerokolekt“ uređaja je
zasnovan na strujanju vazduha kroz jednokratan čip iz kog se u laboratoriji
direktnom elucijom dobija uzorak nukleinske kiseline pogodan za PCR
analize. Uzorci su sakupljeni sa 5 farmi svinja koje imaju potvrđeno prisustvo
navedenih virusnih patogena. Rezultati ispitivanja vazduha su poređeni sa
rezultatima dobijenim iz uobičajeno sakupljenih uzoraka. Preliminarnim
rezultatima je utvrđeno da je ispitivanje vazduha pogodno za utvrđivanje
prisustva navedenih uzročnika na farmi. Primenom metodologije uzorkovanja
vazduha, može se vršiti aktivan nadzor na prisustvo patogena, održavanje
slobodnog statusa od bolesti, kao i rana detekcija patogena. Značajne
prednosti ispitivanja vazduha su i očuvanje dobrobiti, usled izostanka
direktnog kontakta sa životinjama., Many infectious diseases are transmitted by air. Individuals which show clinical signs
of respiratory diseases excrete the culprit, and through expectoration in the form of
droplets, the causative agents end up in the external environment where they can
remain be protected within the droplets. Enteral pathogens can also be transmitted by
air, through dust particles lifted by active animal and farm staff movement. Noninvasive sampling methods are significant for swift and simple insight into the health
status of a herd, excluding individual variations, and withal not disrupting regular
farm activities. Earlier it was shown that oral fluid samples, acquired by non-invasive
methods, are a reliable way of determining the status of a herd. Meanwhile, our study
tested the presence of viral swine pathogens in the air, including the swine influenza
virus, circovirus type two, and the respiratory and reproductive syndrome virus. The
air was sampled using the “Aerocollect” device. The working principle of the
“Aerocollect” apparatus is based on the flow of air through a single-use chip from
which nucleic acid can be eluted and used for PCR analysis. The samples were
collected from five pig farms, which had a confirmed presence of the beforementioned
viral swine pathogens. The results of the analysis were compared with results from
traditionally collected samples. The preliminary results confirm that air sampling can
be used for determining the presence of the mentioned pathogens on a farm. By
applying air sampling methodology, active surveillance for the presence of pathogens
can be conducted, as well as the maintenance of free disease status, and early pathogen
detection. Considerable advantages of air sampling are the preservation of animal
welfare and the absence of direct animal contact.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani)",
title = "Virusni patogeni svinja u vazduhu, Viral swine pathogens in the air",
pages = "85-84"
}

Glišić, D., Veljović, L., Maletić, J., Kureljušić, B., Ninković, M., Milovanović, B.,& Milićević, V.. (2023). Virusni patogeni svinja u vazduhu. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani)
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 84-85.

Glišić D, Veljović L, Maletić J, Kureljušić B, Ninković M, Milovanović B, Milićević V. Virusni patogeni svinja u vazduhu. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani). 2023;:84-85..

Glišić, Dimitrije, Veljović, Ljubiša, Maletić, Jelena, Kureljušić, Branislav, Ninković, Milan, Milovanović, Bojan, Milićević, Vesna, "Virusni patogeni svinja u vazduhu" in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani) (2023):84-85.

TY  - CONF
AU  - Vasić, Ana
AU  - Kureljušić, Jasna
AU  - Rokvić, Nikola
AU  - Milovanović, Bojan
AU  - Glišić, Dimitrije
AU  - Radanović, Oliver
AU  - Pavlović, Ivan
AU  - Paunović, Milan
AU  - Kureljušić, Branislav
AU  - Milićević, Vesna
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/538
AB  - Nutrije (Myocastor coypus) su invazivna južnoamerička vrsta velikih glodara
čija je populacija na teritoriji Republike Srbije stabilna i brojna. Nekada
gajeni zbog krzna, danas naseljavaju sve kontinente osim Okeanije i
Antarktika. Nutrije žive na vlažnim staništima, uz obale većih reka i
drugih vodenih površina. Iako su pretežno biljojedi, mogu ce hraniti i
hranom životinjskog porekla, kao što su lešine, insekti, mali sisari i
ptice, ribe i mekušci. U zemljama gde se koriste u ishrani ljudi opisani su
slučajevi infekcije sa Trichinella spp. posle konzumiranja mesa nutrija. O
prisustvu parazita kod nutrija postoje oskudni podaci iz literature, a među
zabeleženim vrstama parazita po značaju se ističe Echinococcus multilocularis
čija su rezervoarna vrsta u prirodi. Cilj našeg rada je bio da sagledamo
parazitsku faunu nutrija Srbije, invazivne vrste o čijoj se biologiji i
bolestima malo zna.
Tokom 2022. i 2023. godine, legalno je izlovljen ukupno 101 primerak (15
juvenilnih, 8 subadulta i 78 adulta) nutrija, koje su dostavljene Odeljenju za
patologiju Naučnog instituta za veterinarstvo Srbije. Spoljašnjim
pregledom, nije utvrđeno prisustvo ektoparazita. Posle izvršene obdukcije,
iz creva su izolovani adulti crevnih parazita i izvršena je morfološka
identifikacija do nivoa roda i vrsta. Preliminarni rezultati su pokazali
prisustvo unutrašnjih parazita kod 12 primeraka i to kod 11 adulta i 1
juvenilnog primerka. Morfološkom determinacijom ustanovljeno je
prisustvo adulta Echinococcus multilocularis. Takođe, uzeti su uzorci jezika
i/ili žvakaćih mišića od ukupno 90 nutrija radi pregleda na prisustvo larvi
Trichinella spp. metodom veštačke digestije. Prisustvo larvi Trichinella spp.
nije utvrđeno u pregledanim uzorcima.
Po našem saznanju, ovo su prvi dokazi prisustva parazita Echinococcus
multilocularis i odsustva larvi Trichinella spp. u populaciji nutrija u
Republici Srbiji. Radi boljeg sagledavanja uloge ove vrste u prirodnim
ciklusima parazita, potrebna su dalja istraživanja.
AB  - Nutria (Myocastor coypus) is an invasive rodent species native for South America,
whose population is stable and numerous in the territory of the Republic of Serbia.
This species has been bred by humans because of its fur for decades before it became
widespread in all continents apart from Antarctic and Oceania. Nutria lives in
wetlands, along the banks of larger rivers and other water bodies. Although they are
predominately herbivores, they can also feed on food of animal origin, as carcasses,
insects, small mammals and birds, fish or molluscs. In the countries where nutria is
used as food, the infections of humans with Trichinella spp. were described. There is
limited literature data on the presence of parasites in nutria, but with existing records
on important parasites such as Echinococcus multilocularis for which nutria is
reservoir species. The aim of our research was to get insight in the parasitic fauna of
nutria in Serbia, an invasive species whose biology and diseases remain unstudied.
During 2022 and 2023, total of 101 nutria specimens were legally hunted (15 juvenile,
8 subadults and 78 adults), and transported to Pathology department of the Scientific
Veterinary Institute of Serbia. After outer inspection of the specimens no ectoparasites
were found. After autopsy, from the intestine adult parasites were isolated and a
morphological determination of species was performed. The parasites were found in
12 specimens from which 11 were adults and 1 was juvenile. Using morphological
determination, the presence of adult Echinococcus multilocularis was established.
Furthermore, samples of tongue and/or jaw musculature were taken from 90
specimens for the determination of presence of Trichinella spp. larvae using artificial
digestion method. There was no Trichinella spp. present in our samples.
To the best of our knowledge, these are the first evidence of Echinococcus
multilocularis presence and Trichinella spp. absence in nutria population of Serbia.
To better understand the role of this species in natural cycles of parasites, further
research is needed.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani)
T1  - Parazitska fauna nutrija (Myocastor coypus) na teritoriji Republike Srbije
T1  - Parasitic fauna of nutria (Myocastor coypus) in the Republic of Serbia
EP  - 77
SP  - 76
ER  - 

@conference{
author = "Vasić, Ana and Kureljušić, Jasna and Rokvić, Nikola and Milovanović, Bojan and Glišić, Dimitrije and Radanović, Oliver and Pavlović, Ivan and Paunović, Milan and Kureljušić, Branislav and Milićević, Vesna",
year = "2023",
abstract = "Nutrije (Myocastor coypus) su invazivna južnoamerička vrsta velikih glodara
čija je populacija na teritoriji Republike Srbije stabilna i brojna. Nekada
gajeni zbog krzna, danas naseljavaju sve kontinente osim Okeanije i
Antarktika. Nutrije žive na vlažnim staništima, uz obale većih reka i
drugih vodenih površina. Iako su pretežno biljojedi, mogu ce hraniti i
hranom životinjskog porekla, kao što su lešine, insekti, mali sisari i
ptice, ribe i mekušci. U zemljama gde se koriste u ishrani ljudi opisani su
slučajevi infekcije sa Trichinella spp. posle konzumiranja mesa nutrija. O
prisustvu parazita kod nutrija postoje oskudni podaci iz literature, a među
zabeleženim vrstama parazita po značaju se ističe Echinococcus multilocularis
čija su rezervoarna vrsta u prirodi. Cilj našeg rada je bio da sagledamo
parazitsku faunu nutrija Srbije, invazivne vrste o čijoj se biologiji i
bolestima malo zna.
Tokom 2022. i 2023. godine, legalno je izlovljen ukupno 101 primerak (15
juvenilnih, 8 subadulta i 78 adulta) nutrija, koje su dostavljene Odeljenju za
patologiju Naučnog instituta za veterinarstvo Srbije. Spoljašnjim
pregledom, nije utvrđeno prisustvo ektoparazita. Posle izvršene obdukcije,
iz creva su izolovani adulti crevnih parazita i izvršena je morfološka
identifikacija do nivoa roda i vrsta. Preliminarni rezultati su pokazali
prisustvo unutrašnjih parazita kod 12 primeraka i to kod 11 adulta i 1
juvenilnog primerka. Morfološkom determinacijom ustanovljeno je
prisustvo adulta Echinococcus multilocularis. Takođe, uzeti su uzorci jezika
i/ili žvakaćih mišića od ukupno 90 nutrija radi pregleda na prisustvo larvi
Trichinella spp. metodom veštačke digestije. Prisustvo larvi Trichinella spp.
nije utvrđeno u pregledanim uzorcima.
Po našem saznanju, ovo su prvi dokazi prisustva parazita Echinococcus
multilocularis i odsustva larvi Trichinella spp. u populaciji nutrija u
Republici Srbiji. Radi boljeg sagledavanja uloge ove vrste u prirodnim
ciklusima parazita, potrebna su dalja istraživanja., Nutria (Myocastor coypus) is an invasive rodent species native for South America,
whose population is stable and numerous in the territory of the Republic of Serbia.
This species has been bred by humans because of its fur for decades before it became
widespread in all continents apart from Antarctic and Oceania. Nutria lives in
wetlands, along the banks of larger rivers and other water bodies. Although they are
predominately herbivores, they can also feed on food of animal origin, as carcasses,
insects, small mammals and birds, fish or molluscs. In the countries where nutria is
used as food, the infections of humans with Trichinella spp. were described. There is
limited literature data on the presence of parasites in nutria, but with existing records
on important parasites such as Echinococcus multilocularis for which nutria is
reservoir species. The aim of our research was to get insight in the parasitic fauna of
nutria in Serbia, an invasive species whose biology and diseases remain unstudied.
During 2022 and 2023, total of 101 nutria specimens were legally hunted (15 juvenile,
8 subadults and 78 adults), and transported to Pathology department of the Scientific
Veterinary Institute of Serbia. After outer inspection of the specimens no ectoparasites
were found. After autopsy, from the intestine adult parasites were isolated and a
morphological determination of species was performed. The parasites were found in
12 specimens from which 11 were adults and 1 was juvenile. Using morphological
determination, the presence of adult Echinococcus multilocularis was established.
Furthermore, samples of tongue and/or jaw musculature were taken from 90
specimens for the determination of presence of Trichinella spp. larvae using artificial
digestion method. There was no Trichinella spp. present in our samples.
To the best of our knowledge, these are the first evidence of Echinococcus
multilocularis presence and Trichinella spp. absence in nutria population of Serbia.
To better understand the role of this species in natural cycles of parasites, further
research is needed.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani)",
title = "Parazitska fauna nutrija (Myocastor coypus) na teritoriji Republike Srbije, Parasitic fauna of nutria (Myocastor coypus) in the Republic of Serbia",
pages = "77-76"
}

Vasić, A., Kureljušić, J., Rokvić, N., Milovanović, B., Glišić, D., Radanović, O., Pavlović, I., Paunović, M., Kureljušić, B.,& Milićević, V.. (2023). Parazitska fauna nutrija (Myocastor coypus) na teritoriji Republike Srbije. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani)
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 76-77.

Vasić A, Kureljušić J, Rokvić N, Milovanović B, Glišić D, Radanović O, Pavlović I, Paunović M, Kureljušić B, Milićević V. Parazitska fauna nutrija (Myocastor coypus) na teritoriji Republike Srbije. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani). 2023;:76-77..

Vasić, Ana, Kureljušić, Jasna, Rokvić, Nikola, Milovanović, Bojan, Glišić, Dimitrije, Radanović, Oliver, Pavlović, Ivan, Paunović, Milan, Kureljušić, Branislav, Milićević, Vesna, "Parazitska fauna nutrija (Myocastor coypus) na teritoriji Republike Srbije" in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani) (2023):76-77.

TY  - CONF
AU  - Kureljušić, Branislav
AU  - Savić, Božidar
AU  - Jezdimirović, Nemanja
AU  - Milovanović, Bojan
AU  - Maksimović Zorić, Jelena
AU  - Dobrosavljević, Ivan
AU  - Prodanov Radulović, Jasna
AU  - Glišić, Dimitrije
AU  - Milićević, Vesna
AU  - Bojkovski, Jovan
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/499
AB  - Bolesti svinja koje se karakterišu pojavom vezikularnog sindroma su slinavka i šap,
vezikularna bolest svinja, vezikularni stomatitis, vezikularni egzantem svinja i Seneka bolest
svinja. Virus slinavke i šapa, virus vezikularne bolesti svinja i Senecavirus A pripadaju familiji
Picornaviridae.
Slinavka i šap je visoko kontagiozna virusna bolest različitih vrsta papkara koja se kod
svinja karakteriše apatijom, febrom, pojavom vezikula u predelu rila, usne šupljine, papaka i
mlečne žlezde. Klinička slika kod svinja uobičajeno nije teška, a u težim slučajevima može se
javiti eksungulacija. Vrata infekcije su sluznica ždrela i tonzile gde se odvija primarna replikacija
virusa, a potom sledi viremija sa sekundarnom replikacijom virusa na predilekcionim mestima.
Poznato je da inficirana svinja može da proizvede 400 miliona infektivnih doza dnevno i da tako
predstavlja značajan izvor infekcije za druge prijemčive vrste.
Infekcija Senecavirusom A se karakteriše povišenim mortalitetom kod prsadi na sisi do
sedmog dana starosti. Kod priplodnih kategorija svinja, postoji povišena telesna temperatura,
anoreksija, letargija, pojava vezikula na rilu, sluznici usne šupljine i koronarnom rubu papaka.
Pojava bolesti kod svinja koje se karakterišu vezikularnim sindromom uvek izaziva veliku
zabrinutost imajući u vidu da je klinički nemoguće razlikovati lezije koje izaziva virus slinavke i
šapa i drugi virusi. Konačnu dijagnozu je moguće jedino potvrditi u laboratoriji. Obzirom da su
svinje prijemčive za više vrsta virusa koji dovode do vezikularnog sindroma kao i činjenicu da
svinje pozitivne na slinavku i šap izlučuju veliku količinu virusa u spoljašnju sredinu putem
aerosola, blagovremeno utvrđivanje dijagnoze je od prvorazrednog značaja.
Ukoliko postoji sumnja na neku od bolesti koje se karakterišu vezikularnim sindromom
potrebno je bez odlaganja obavestiti nadležnog veterinarskog inspektora i regionalni institut koji
zajedno sa terenskom veterinarskom službom utvrđuju dalji postupak u skladu sa zakonskom
regulativom i sprovode epizootiološki nadzor i uzorkovanje materijala za laboratorijska
ispitivanja. Uzorci prvog prioriteta su pokrovni epitel vezikula, sadržaj vezikula, oralni/nazalni brisevi i uzorci krvi. Laboratorijska dijagnostika omogućava dobijanje rezultata u roku od
nekoliko časova.
PB  - Požarevac : Sitograf RM
C3  - 20. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja
T1  - Vezikularni sindrom kod svinja – slinavka i šap ili Seneka virus?
EP  - 78
SP  - 72
ER  - 

@conference{
author = "Kureljušić, Branislav and Savić, Božidar and Jezdimirović, Nemanja and Milovanović, Bojan and Maksimović Zorić, Jelena and Dobrosavljević, Ivan and Prodanov Radulović, Jasna and Glišić, Dimitrije and Milićević, Vesna and Bojkovski, Jovan",
year = "2023",
abstract = "Bolesti svinja koje se karakterišu pojavom vezikularnog sindroma su slinavka i šap,
vezikularna bolest svinja, vezikularni stomatitis, vezikularni egzantem svinja i Seneka bolest
svinja. Virus slinavke i šapa, virus vezikularne bolesti svinja i Senecavirus A pripadaju familiji
Picornaviridae.
Slinavka i šap je visoko kontagiozna virusna bolest različitih vrsta papkara koja se kod
svinja karakteriše apatijom, febrom, pojavom vezikula u predelu rila, usne šupljine, papaka i
mlečne žlezde. Klinička slika kod svinja uobičajeno nije teška, a u težim slučajevima može se
javiti eksungulacija. Vrata infekcije su sluznica ždrela i tonzile gde se odvija primarna replikacija
virusa, a potom sledi viremija sa sekundarnom replikacijom virusa na predilekcionim mestima.
Poznato je da inficirana svinja može da proizvede 400 miliona infektivnih doza dnevno i da tako
predstavlja značajan izvor infekcije za druge prijemčive vrste.
Infekcija Senecavirusom A se karakteriše povišenim mortalitetom kod prsadi na sisi do
sedmog dana starosti. Kod priplodnih kategorija svinja, postoji povišena telesna temperatura,
anoreksija, letargija, pojava vezikula na rilu, sluznici usne šupljine i koronarnom rubu papaka.
Pojava bolesti kod svinja koje se karakterišu vezikularnim sindromom uvek izaziva veliku
zabrinutost imajući u vidu da je klinički nemoguće razlikovati lezije koje izaziva virus slinavke i
šapa i drugi virusi. Konačnu dijagnozu je moguće jedino potvrditi u laboratoriji. Obzirom da su
svinje prijemčive za više vrsta virusa koji dovode do vezikularnog sindroma kao i činjenicu da
svinje pozitivne na slinavku i šap izlučuju veliku količinu virusa u spoljašnju sredinu putem
aerosola, blagovremeno utvrđivanje dijagnoze je od prvorazrednog značaja.
Ukoliko postoji sumnja na neku od bolesti koje se karakterišu vezikularnim sindromom
potrebno je bez odlaganja obavestiti nadležnog veterinarskog inspektora i regionalni institut koji
zajedno sa terenskom veterinarskom službom utvrđuju dalji postupak u skladu sa zakonskom
regulativom i sprovode epizootiološki nadzor i uzorkovanje materijala za laboratorijska
ispitivanja. Uzorci prvog prioriteta su pokrovni epitel vezikula, sadržaj vezikula, oralni/nazalni brisevi i uzorci krvi. Laboratorijska dijagnostika omogućava dobijanje rezultata u roku od
nekoliko časova.",
publisher = "Požarevac : Sitograf RM",
journal = "20. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja",
title = "Vezikularni sindrom kod svinja – slinavka i šap ili Seneka virus?",
pages = "78-72"
}

Kureljušić, B., Savić, B., Jezdimirović, N., Milovanović, B., Maksimović Zorić, J., Dobrosavljević, I., Prodanov Radulović, J., Glišić, D., Milićević, V.,& Bojkovski, J.. (2023). Vezikularni sindrom kod svinja – slinavka i šap ili Seneka virus?. in 20. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja
Požarevac : Sitograf RM., 72-78.

Kureljušić B, Savić B, Jezdimirović N, Milovanović B, Maksimović Zorić J, Dobrosavljević I, Prodanov Radulović J, Glišić D, Milićević V, Bojkovski J. Vezikularni sindrom kod svinja – slinavka i šap ili Seneka virus?. in 20. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja. 2023;:72-78..

Kureljušić, Branislav, Savić, Božidar, Jezdimirović, Nemanja, Milovanović, Bojan, Maksimović Zorić, Jelena, Dobrosavljević, Ivan, Prodanov Radulović, Jasna, Glišić, Dimitrije, Milićević, Vesna, Bojkovski, Jovan, "Vezikularni sindrom kod svinja – slinavka i šap ili Seneka virus?" in 20. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja (2023):72-78.

TY  - CONF
AU  - Savić, Božidar
AU  - Radanović, Oliver
AU  - Kureljušić, Branislav
AU  - Zdravković, Nemanja
AU  - Jezdimirović, Nemanja
AU  - Milovanović, Bojan
AU  - Maksimović Zorić, Jelena
AU  - Milićević, Vesna
AU  - Pavlović, Ivan
AU  - Stevančević, Ognjen
AU  - Dobrosavljević, Ivan
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/504
AB  - Streptokokone infekcije svinja se pojavljuju u svim zemljama sa intenzivnom svinjarskom
proizvodnjom, a izazivaju ih patogene streptokoke različitih serogrupa. Nekoliko vrsta
streptokoka je ustanovljeno kod svinja, od kojih su neki komensali, a neki patogeni. Najznačajnija
i najrasprostranjenija streptokoka svinja je Streptococcus suis (S. suis), uzročnik septikemičnog
oboljenja ili promena lokalizovanih u organima kod prasadi na sisi, prasadi stare 5 do 10 nedelja
i starijih kategorija svinja uključujući i nazimice, krmače i nerastove. Streptococcus suis je
inkapsulirana Gram-pozitivna koka, svrstana u grupu D prema Lancefield klasifikaciji. Do danas
je utvrđeno 35 kapsluarnih serotiova S. suis, koji se međusobno razlikuju po patogenosti. Većina
sojeva S. suis izolovanih od obolelih svinja pripada serotipovima 1-9, pri čemu je serotip 2
najprevalentniji i najvirulentniji serotip u većini evroazijskih zemalja. Streptokoke mogu na
različite načine da dospeju u organizam životinja. Glavna „ulazna vrata“ infekcije kod prasadi na
sisi predstavlja pupak, kratko posle poroda, ledirana gingiva ili otvorena pulpa kod korekcije zuba,
abrazije kože na ekstremitetima kod neadekvatnih podova, a naročito kada je kod krmača prisutan
MMA sindrom odnosno sindrom postpartalne disgalakcije. Takođe, i lezije kod kupiranja repa i
kastracije muške prasadi mogu predstavljati atrijum za infekciju. Kod latentno inficiranih krmača,
streptokoke naseljavaju tonzile pa se pretpostavlja da se u ovakvim slučajevima prasad mogu
inficirati preko respiratornog sistema. Ako se prasad inficiraju od latentno inficiranih krmača do
ekspresije bolesti dolazi posle imunosupresije.
Prateći zdravstveno stanje svinja u velikim aglomeracijama nam je omogućilo da na celovit način
pratimo oboljenja streptokokne etiologije koja se pojavljuju u više kliničkih i patomorfoloških
oblika. Tako, streptokokna infekcija kod prasadi na sisi i zalučene prasadi se manifestuje kao
streptokokna septikemija novorođene prasadi, streptokokna septikemija prasadi stare 2-3 nedelje,
zatim kao artritis, osteomijelitis, endokarditis, apostematozni miokarditis, meningoencefalitis,
cefalocervikalni limfadenitis, supkutani periartikularni absces, pneumonija i zapaljenje srednjeg
uva. Od oblika streptokokne infekcije kod prasadi na sisi najfrekentnije se javljaju streptokokna
septikemija i artritisi, a kod prasadi iz odgoja meningo-encefalitisi, endokarditis, pneumonija i  
zapaljenje srednjeg uva. Vrlo retko smo imali priliku da detektujemo i cefalo-cervikalni
limfadenitis i periartikularne apcese. Kod krmača, streptokoke su bile odgovorne za razvoj
mastitisa, endometritisa i pojavu abortusa. Najfrekventnije izolovani serotip S. suis u našem
istraživanju je bio serotip 2 (45%), a pored njega detektovani su i serotipovi ½ (17%), 1 (13%), 4
(7%), 7 (6%), 9 (5%), 5 (4%) i serotip 3 (3%), kao i veći broj izolata koje nismo mogli tipizirati.
Streptokokna infekcija svinja predstavlja ozbiljan i značajan zdravstveni problem u većim
aglomeracijama, a karakterišu je različiti putevi infekcije kao i polimorfizom u izražavanju.
AB  - Streptococcal infections of pigs occur in all countries with intensive pig production, and
are caused by pathogenic streptococci of different serogroups. Several species of streptococci have
been found in pigs, some of which are commensal and some are pathogenic. Streptococcus suis
(S. Suis) is one of the most important and widespread pathogens of pigs causing septicemic disease
or localized lesions in different organs in suckling piglets, 5- to 10-week-old piglets, and older
categories of pigs including gilts, sows, and boars. Streptococcus suis is an encapsulated Grampositive cocci, classified in group D according to the Lancefield classification. A total of 35 S.
suis capsular serotypes have been identified to date, which differ in pathogenicity. Most S. suis
strains isolated from diseased pigs belong to serotypes 1-9, with serotype 2 being the most
prevalent and virulent serotype in most eurasian countries. Streptococci can enter into the body in
different ways. The main portals of infection in suckling piglets are umbilical infections shortly
after birth, gum lesions or exposed dental pulp made during tooth cutting, skin injuries on the
extremities caused by abrasive floors, especially when MMA or postpartum dysgalactia syndrome
is present in sows. Castration and tail docking wounds can also be an portals for infection. In
latently infected sows, streptococci inhabit the tonsils, thus it is assumed that piglets can be
 infected through the respiratory system. In cases of infection of piglets from latently infected
sows, the disease occur in piglets after immunosuppression.
Monitoring the health status of pigs in large agglomerations allowed us, to comprehensively
survey of clinical, pathomorphological and etiological features of streptococcal diseases. Thus,
septicemia of newborn piglets, septicemia of 2-3 week old piglets, arthritis, osteomyelitis,
endocarditis, apostematous myocarditis, meningoencephalitis, cephalocervical lymphadenitis,
subcutaneous periarticular abscess, pneumonia and otitis media, were the most severe
manifestation of the disease. The septicemia and arthritis were the most common forms of the
streptococcal disease in suckling piglets, while meningo-encephalitis, endocarditis, pneumonia
and otitis media were the most common streptococcal diseases in weaned piglets. Cephalocervical lymphadenitis and periarticular abscesses were relativly sparsely detected. The most frequently isolated serotype S. suis was serotype 2 (45%), in addition, serotypes ½ (17%), 1 (13%), 4 (7%), 7 (6%), 9 (5%), 5 (4%) and 3 (3%) were also detected, as well as a larger number of isolates that we could not type. Streptococcal infection represents severe and significant health
problem in large pig agglomerations, and is characterized by different routes of infection as well
as polymorphism in expression.
PB  - Požarevac : Sitograf RM
C3  - 20. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja
T1  - Kliničke i patomorfološke karakteristike infekcije svinja sa Streptococcus suis
T1  - Clinical and pathological characteristics of Streptococcus suis infections in pigs
EP  - 71
SP  - 56
ER  - 

@conference{
author = "Savić, Božidar and Radanović, Oliver and Kureljušić, Branislav and Zdravković, Nemanja and Jezdimirović, Nemanja and Milovanović, Bojan and Maksimović Zorić, Jelena and Milićević, Vesna and Pavlović, Ivan and Stevančević, Ognjen and Dobrosavljević, Ivan",
year = "2023",
abstract = "Streptokokone infekcije svinja se pojavljuju u svim zemljama sa intenzivnom svinjarskom
proizvodnjom, a izazivaju ih patogene streptokoke različitih serogrupa. Nekoliko vrsta
streptokoka je ustanovljeno kod svinja, od kojih su neki komensali, a neki patogeni. Najznačajnija
i najrasprostranjenija streptokoka svinja je Streptococcus suis (S. suis), uzročnik septikemičnog
oboljenja ili promena lokalizovanih u organima kod prasadi na sisi, prasadi stare 5 do 10 nedelja
i starijih kategorija svinja uključujući i nazimice, krmače i nerastove. Streptococcus suis je
inkapsulirana Gram-pozitivna koka, svrstana u grupu D prema Lancefield klasifikaciji. Do danas
je utvrđeno 35 kapsluarnih serotiova S. suis, koji se međusobno razlikuju po patogenosti. Većina
sojeva S. suis izolovanih od obolelih svinja pripada serotipovima 1-9, pri čemu je serotip 2
najprevalentniji i najvirulentniji serotip u većini evroazijskih zemalja. Streptokoke mogu na
različite načine da dospeju u organizam životinja. Glavna „ulazna vrata“ infekcije kod prasadi na
sisi predstavlja pupak, kratko posle poroda, ledirana gingiva ili otvorena pulpa kod korekcije zuba,
abrazije kože na ekstremitetima kod neadekvatnih podova, a naročito kada je kod krmača prisutan
MMA sindrom odnosno sindrom postpartalne disgalakcije. Takođe, i lezije kod kupiranja repa i
kastracije muške prasadi mogu predstavljati atrijum za infekciju. Kod latentno inficiranih krmača,
streptokoke naseljavaju tonzile pa se pretpostavlja da se u ovakvim slučajevima prasad mogu
inficirati preko respiratornog sistema. Ako se prasad inficiraju od latentno inficiranih krmača do
ekspresije bolesti dolazi posle imunosupresije.
Prateći zdravstveno stanje svinja u velikim aglomeracijama nam je omogućilo da na celovit način
pratimo oboljenja streptokokne etiologije koja se pojavljuju u više kliničkih i patomorfoloških
oblika. Tako, streptokokna infekcija kod prasadi na sisi i zalučene prasadi se manifestuje kao
streptokokna septikemija novorođene prasadi, streptokokna septikemija prasadi stare 2-3 nedelje,
zatim kao artritis, osteomijelitis, endokarditis, apostematozni miokarditis, meningoencefalitis,
cefalocervikalni limfadenitis, supkutani periartikularni absces, pneumonija i zapaljenje srednjeg
uva. Od oblika streptokokne infekcije kod prasadi na sisi najfrekentnije se javljaju streptokokna
septikemija i artritisi, a kod prasadi iz odgoja meningo-encefalitisi, endokarditis, pneumonija i  
zapaljenje srednjeg uva. Vrlo retko smo imali priliku da detektujemo i cefalo-cervikalni
limfadenitis i periartikularne apcese. Kod krmača, streptokoke su bile odgovorne za razvoj
mastitisa, endometritisa i pojavu abortusa. Najfrekventnije izolovani serotip S. suis u našem
istraživanju je bio serotip 2 (45%), a pored njega detektovani su i serotipovi ½ (17%), 1 (13%), 4
(7%), 7 (6%), 9 (5%), 5 (4%) i serotip 3 (3%), kao i veći broj izolata koje nismo mogli tipizirati.
Streptokokna infekcija svinja predstavlja ozbiljan i značajan zdravstveni problem u većim
aglomeracijama, a karakterišu je različiti putevi infekcije kao i polimorfizom u izražavanju., Streptococcal infections of pigs occur in all countries with intensive pig production, and
are caused by pathogenic streptococci of different serogroups. Several species of streptococci have
been found in pigs, some of which are commensal and some are pathogenic. Streptococcus suis
(S. Suis) is one of the most important and widespread pathogens of pigs causing septicemic disease
or localized lesions in different organs in suckling piglets, 5- to 10-week-old piglets, and older
categories of pigs including gilts, sows, and boars. Streptococcus suis is an encapsulated Grampositive cocci, classified in group D according to the Lancefield classification. A total of 35 S.
suis capsular serotypes have been identified to date, which differ in pathogenicity. Most S. suis
strains isolated from diseased pigs belong to serotypes 1-9, with serotype 2 being the most
prevalent and virulent serotype in most eurasian countries. Streptococci can enter into the body in
different ways. The main portals of infection in suckling piglets are umbilical infections shortly
after birth, gum lesions or exposed dental pulp made during tooth cutting, skin injuries on the
extremities caused by abrasive floors, especially when MMA or postpartum dysgalactia syndrome
is present in sows. Castration and tail docking wounds can also be an portals for infection. In
latently infected sows, streptococci inhabit the tonsils, thus it is assumed that piglets can be
 infected through the respiratory system. In cases of infection of piglets from latently infected
sows, the disease occur in piglets after immunosuppression.
Monitoring the health status of pigs in large agglomerations allowed us, to comprehensively
survey of clinical, pathomorphological and etiological features of streptococcal diseases. Thus,
septicemia of newborn piglets, septicemia of 2-3 week old piglets, arthritis, osteomyelitis,
endocarditis, apostematous myocarditis, meningoencephalitis, cephalocervical lymphadenitis,
subcutaneous periarticular abscess, pneumonia and otitis media, were the most severe
manifestation of the disease. The septicemia and arthritis were the most common forms of the
streptococcal disease in suckling piglets, while meningo-encephalitis, endocarditis, pneumonia
and otitis media were the most common streptococcal diseases in weaned piglets. Cephalocervical lymphadenitis and periarticular abscesses were relativly sparsely detected. The most frequently isolated serotype S. suis was serotype 2 (45%), in addition, serotypes ½ (17%), 1 (13%), 4 (7%), 7 (6%), 9 (5%), 5 (4%) and 3 (3%) were also detected, as well as a larger number of isolates that we could not type. Streptococcal infection represents severe and significant health
problem in large pig agglomerations, and is characterized by different routes of infection as well
as polymorphism in expression.",
publisher = "Požarevac : Sitograf RM",
journal = "20. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja",
title = "Kliničke i patomorfološke karakteristike infekcije svinja sa Streptococcus suis, Clinical and pathological characteristics of Streptococcus suis infections in pigs",
pages = "71-56"
}

Savić, B., Radanović, O., Kureljušić, B., Zdravković, N., Jezdimirović, N., Milovanović, B., Maksimović Zorić, J., Milićević, V., Pavlović, I., Stevančević, O.,& Dobrosavljević, I.. (2023). Kliničke i patomorfološke karakteristike infekcije svinja sa Streptococcus suis. in 20. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja
Požarevac : Sitograf RM., 56-71.

Savić B, Radanović O, Kureljušić B, Zdravković N, Jezdimirović N, Milovanović B, Maksimović Zorić J, Milićević V, Pavlović I, Stevančević O, Dobrosavljević I. Kliničke i patomorfološke karakteristike infekcije svinja sa Streptococcus suis. in 20. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja. 2023;:56-71..

Savić, Božidar, Radanović, Oliver, Kureljušić, Branislav, Zdravković, Nemanja, Jezdimirović, Nemanja, Milovanović, Bojan, Maksimović Zorić, Jelena, Milićević, Vesna, Pavlović, Ivan, Stevančević, Ognjen, Dobrosavljević, Ivan, "Kliničke i patomorfološke karakteristike infekcije svinja sa Streptococcus suis" in 20. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja (2023):56-71.