A sample preparation methodology using different d-spe sorbent for amitraz analysis in honey
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
An analytical method was validated using gas chromatography with tandem mass
spectrometry (GC/MS) for the precise analysis of amitraz and its metabolites 2, 4-
dimethylaniline (2, 4 - DMA) in honey. The QuEChERS technique, which comes from the
words Quick, Easy, Cheap, Effective, Rugged, and Safe extraction of the pesticides was used.
Amitraz is both an acaricide and insecticide and is often used in beekeeping to protect
honeybee colonies against Varroa destructor. The procedure was validated according to
SANTE/11325/2021 and the values of recovery, precision, and linearity, limit of detection
and limit of quantification were established. The average recoveries obtained for amitraz were
ranged from 79.4 to 100.8% and for 2, 4 dimethylaniline from 78.1% to 103.6 % at the two
spiked levels 0.01 and 0.02 mg/kg. Several clean-up approaches were tested: d-SPE with
Enhanced Matrix Removal-Lipid (EMR-Lipid), combination of anhydrous magnesium
sulfate, primary secondary amine (PSA) ...and C18 sorbents, and a third method using Z-Sep.
The most precise recovery, i.e. the highest accuracy, was achieved using purification with
EMR lipid. The linearity of the analytical response across the studied range of concentrations
(0.010 - 0.10 mg/kg) was excellent, obtaining correlation coefficients higher than 0.99. Limit
of quantification was 0.004 mg/kg for amitraz and 0.005 mg/kg for 2, 4-DMA. The method
was used for the determination of amitraz and 2, 4-DMA in real samples of acacia and flower
honey.
Кључне речи:
honey / QuEChERS method / GC/MS / amitrazИзвор:
Book of Proceedings : XIV International Scientific Agricultural Symposium “Agrosym 2023”, 2023, 624-629Издавач:
- East Sarajevo : Faculty of Agriculture
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200030 (Научни институт за ветеринарство Србије, Београд) (RS-MESTD-inst-2020-200030)
Институција/група
Naučni institut za veterinarstvo SrbijeTY - CONF AU - Tasić, Aleksandra AU - Pavlović, Ivan AU - Bijelić, Tanja PY - 2023 UR - https://reponivs.nivs.rs/handle/123456789/839 AB - An analytical method was validated using gas chromatography with tandem mass spectrometry (GC/MS) for the precise analysis of amitraz and its metabolites 2, 4- dimethylaniline (2, 4 - DMA) in honey. The QuEChERS technique, which comes from the words Quick, Easy, Cheap, Effective, Rugged, and Safe extraction of the pesticides was used. Amitraz is both an acaricide and insecticide and is often used in beekeeping to protect honeybee colonies against Varroa destructor. The procedure was validated according to SANTE/11325/2021 and the values of recovery, precision, and linearity, limit of detection and limit of quantification were established. The average recoveries obtained for amitraz were ranged from 79.4 to 100.8% and for 2, 4 dimethylaniline from 78.1% to 103.6 % at the two spiked levels 0.01 and 0.02 mg/kg. Several clean-up approaches were tested: d-SPE with Enhanced Matrix Removal-Lipid (EMR-Lipid), combination of anhydrous magnesium sulfate, primary secondary amine (PSA) and C18 sorbents, and a third method using Z-Sep. The most precise recovery, i.e. the highest accuracy, was achieved using purification with EMR lipid. The linearity of the analytical response across the studied range of concentrations (0.010 - 0.10 mg/kg) was excellent, obtaining correlation coefficients higher than 0.99. Limit of quantification was 0.004 mg/kg for amitraz and 0.005 mg/kg for 2, 4-DMA. The method was used for the determination of amitraz and 2, 4-DMA in real samples of acacia and flower honey. PB - East Sarajevo : Faculty of Agriculture C3 - Book of Proceedings : XIV International Scientific Agricultural Symposium “Agrosym 2023” T1 - A sample preparation methodology using different d-spe sorbent for amitraz analysis in honey EP - 629 SP - 624 ER -
@conference{ author = "Tasić, Aleksandra and Pavlović, Ivan and Bijelić, Tanja", year = "2023", abstract = "An analytical method was validated using gas chromatography with tandem mass spectrometry (GC/MS) for the precise analysis of amitraz and its metabolites 2, 4- dimethylaniline (2, 4 - DMA) in honey. The QuEChERS technique, which comes from the words Quick, Easy, Cheap, Effective, Rugged, and Safe extraction of the pesticides was used. Amitraz is both an acaricide and insecticide and is often used in beekeeping to protect honeybee colonies against Varroa destructor. The procedure was validated according to SANTE/11325/2021 and the values of recovery, precision, and linearity, limit of detection and limit of quantification were established. The average recoveries obtained for amitraz were ranged from 79.4 to 100.8% and for 2, 4 dimethylaniline from 78.1% to 103.6 % at the two spiked levels 0.01 and 0.02 mg/kg. Several clean-up approaches were tested: d-SPE with Enhanced Matrix Removal-Lipid (EMR-Lipid), combination of anhydrous magnesium sulfate, primary secondary amine (PSA) and C18 sorbents, and a third method using Z-Sep. The most precise recovery, i.e. the highest accuracy, was achieved using purification with EMR lipid. The linearity of the analytical response across the studied range of concentrations (0.010 - 0.10 mg/kg) was excellent, obtaining correlation coefficients higher than 0.99. Limit of quantification was 0.004 mg/kg for amitraz and 0.005 mg/kg for 2, 4-DMA. The method was used for the determination of amitraz and 2, 4-DMA in real samples of acacia and flower honey.", publisher = "East Sarajevo : Faculty of Agriculture", journal = "Book of Proceedings : XIV International Scientific Agricultural Symposium “Agrosym 2023”", title = "A sample preparation methodology using different d-spe sorbent for amitraz analysis in honey", pages = "629-624" }
Tasić, A., Pavlović, I.,& Bijelić, T.. (2023). A sample preparation methodology using different d-spe sorbent for amitraz analysis in honey. in Book of Proceedings : XIV International Scientific Agricultural Symposium “Agrosym 2023” East Sarajevo : Faculty of Agriculture., 624-629.
Tasić A, Pavlović I, Bijelić T. A sample preparation methodology using different d-spe sorbent for amitraz analysis in honey. in Book of Proceedings : XIV International Scientific Agricultural Symposium “Agrosym 2023”. 2023;:624-629..
Tasić, Aleksandra, Pavlović, Ivan, Bijelić, Tanja, "A sample preparation methodology using different d-spe sorbent for amitraz analysis in honey" in Book of Proceedings : XIV International Scientific Agricultural Symposium “Agrosym 2023” (2023):624-629.