Prevalence of causative agents of subclinical mastitis in dairy cow
Аутори
Radanović, OliverCvetojević, Đorđe
Kureljušić, Branislav
Jezdimirović, Nemanja
Žutić, Jadranka
Остала ауторства
Radosavljević, VladimirКонференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Mastitis is one of the most imprtant economical diseases of
high-yielding cattle. The mastitis is mainly caused by a variety of Gram
positive and negative bacterial species. There are several different
classifications of mastitis. One of the most important is clinical
classification on clinical and subclinical mastitis. Diagnosis of acute,
clinical mastitis is not difficult. Subacute forms of mastitis represent larger
challenge for diagnostic because there are no visible changes in apperance
of secreted milk. In spite of several addition tests for diagnosis of
subclinical mastitis (California mastitis test – CMT, Somatic cell count, etc)
are available, bacterial culture of milk still represent the most reliable
method.
Aim of our study was to investigate presence and prevalence of
different bacterial species isolated from milk with positive CMT reaction.
In total, we examined 9844 samples of milk in period from 2011 to
2015. From each sample, 0,1ml was taken and cultivate...d on Columbia
blood agar in aerobic conditons on 37oC for 24 to 48 hours. Bacteria were
identified by using colony morphology, haemolytic pattern on blood agar
and further microsopic examination (Gram staining), standard biochemical
methods, polymerase chain reaction (PCR), and commercial testS (BBL
Crystal).
From total 9844 samples, bacterial species were isolated in 4381
(44,5%). Most prevalent species were: Staphylococcus aureus in 2382
samples, Streptococcus agalactiae in 892 samples, Streptococcus spp. in
504 samples, Kllebsiela spp. in 163 samples, Escherichia coli in 133
samples, Pseudomonas aeruginosa in 131 samples and Pasteurella
multocida in 91 samples, Corynebacterium bovis in 25 cases and
Trueperella pyogenes in 14 samples. Beside those, in 25 samples we
isolated several different species.
Based upon our results, we concluded that Staphylococcus aureus
and Streptococcus agalactiae represents the most important bacterial
pathogens from cases of subclinical mastitis.
Кључне речи:
subclinical mastitis / dairy cow / prevalence / bacterial pathogensИзвор:
Second International Symposium of Veterinary Medicine (ISVM2016), 2016, 159-159Издавач:
- Belgrade : Scientific Institute of Veterinary Medicine of Serbia
- Novi Sad : Scientific Veterinary Institute "Novi Sad"
Институција/група
Naučni institut za veterinarstvo SrbijeTY - CONF AU - Radanović, Oliver AU - Cvetojević, Đorđe AU - Kureljušić, Branislav AU - Jezdimirović, Nemanja AU - Žutić, Jadranka PY - 2016 UR - https://reponivs.nivs.rs/handle/123456789/674 AB - Mastitis is one of the most imprtant economical diseases of high-yielding cattle. The mastitis is mainly caused by a variety of Gram positive and negative bacterial species. There are several different classifications of mastitis. One of the most important is clinical classification on clinical and subclinical mastitis. Diagnosis of acute, clinical mastitis is not difficult. Subacute forms of mastitis represent larger challenge for diagnostic because there are no visible changes in apperance of secreted milk. In spite of several addition tests for diagnosis of subclinical mastitis (California mastitis test – CMT, Somatic cell count, etc) are available, bacterial culture of milk still represent the most reliable method. Aim of our study was to investigate presence and prevalence of different bacterial species isolated from milk with positive CMT reaction. In total, we examined 9844 samples of milk in period from 2011 to 2015. From each sample, 0,1ml was taken and cultivated on Columbia blood agar in aerobic conditons on 37oC for 24 to 48 hours. Bacteria were identified by using colony morphology, haemolytic pattern on blood agar and further microsopic examination (Gram staining), standard biochemical methods, polymerase chain reaction (PCR), and commercial testS (BBL Crystal). From total 9844 samples, bacterial species were isolated in 4381 (44,5%). Most prevalent species were: Staphylococcus aureus in 2382 samples, Streptococcus agalactiae in 892 samples, Streptococcus spp. in 504 samples, Kllebsiela spp. in 163 samples, Escherichia coli in 133 samples, Pseudomonas aeruginosa in 131 samples and Pasteurella multocida in 91 samples, Corynebacterium bovis in 25 cases and Trueperella pyogenes in 14 samples. Beside those, in 25 samples we isolated several different species. Based upon our results, we concluded that Staphylococcus aureus and Streptococcus agalactiae represents the most important bacterial pathogens from cases of subclinical mastitis. PB - Belgrade : Scientific Institute of Veterinary Medicine of Serbia PB - Novi Sad : Scientific Veterinary Institute "Novi Sad" C3 - Second International Symposium of Veterinary Medicine (ISVM2016) T1 - Prevalence of causative agents of subclinical mastitis in dairy cow EP - 159 SP - 159 ER -
@conference{ author = "Radanović, Oliver and Cvetojević, Đorđe and Kureljušić, Branislav and Jezdimirović, Nemanja and Žutić, Jadranka", year = "2016", abstract = "Mastitis is one of the most imprtant economical diseases of high-yielding cattle. The mastitis is mainly caused by a variety of Gram positive and negative bacterial species. There are several different classifications of mastitis. One of the most important is clinical classification on clinical and subclinical mastitis. Diagnosis of acute, clinical mastitis is not difficult. Subacute forms of mastitis represent larger challenge for diagnostic because there are no visible changes in apperance of secreted milk. In spite of several addition tests for diagnosis of subclinical mastitis (California mastitis test – CMT, Somatic cell count, etc) are available, bacterial culture of milk still represent the most reliable method. Aim of our study was to investigate presence and prevalence of different bacterial species isolated from milk with positive CMT reaction. In total, we examined 9844 samples of milk in period from 2011 to 2015. From each sample, 0,1ml was taken and cultivated on Columbia blood agar in aerobic conditons on 37oC for 24 to 48 hours. Bacteria were identified by using colony morphology, haemolytic pattern on blood agar and further microsopic examination (Gram staining), standard biochemical methods, polymerase chain reaction (PCR), and commercial testS (BBL Crystal). From total 9844 samples, bacterial species were isolated in 4381 (44,5%). Most prevalent species were: Staphylococcus aureus in 2382 samples, Streptococcus agalactiae in 892 samples, Streptococcus spp. in 504 samples, Kllebsiela spp. in 163 samples, Escherichia coli in 133 samples, Pseudomonas aeruginosa in 131 samples and Pasteurella multocida in 91 samples, Corynebacterium bovis in 25 cases and Trueperella pyogenes in 14 samples. Beside those, in 25 samples we isolated several different species. Based upon our results, we concluded that Staphylococcus aureus and Streptococcus agalactiae represents the most important bacterial pathogens from cases of subclinical mastitis.", publisher = "Belgrade : Scientific Institute of Veterinary Medicine of Serbia, Novi Sad : Scientific Veterinary Institute "Novi Sad"", journal = "Second International Symposium of Veterinary Medicine (ISVM2016)", title = "Prevalence of causative agents of subclinical mastitis in dairy cow", pages = "159-159" }
Radanović, O., Cvetojević, Đ., Kureljušić, B., Jezdimirović, N.,& Žutić, J.. (2016). Prevalence of causative agents of subclinical mastitis in dairy cow. in Second International Symposium of Veterinary Medicine (ISVM2016) Belgrade : Scientific Institute of Veterinary Medicine of Serbia., 159-159.
Radanović O, Cvetojević Đ, Kureljušić B, Jezdimirović N, Žutić J. Prevalence of causative agents of subclinical mastitis in dairy cow. in Second International Symposium of Veterinary Medicine (ISVM2016). 2016;:159-159..
Radanović, Oliver, Cvetojević, Đorđe, Kureljušić, Branislav, Jezdimirović, Nemanja, Žutić, Jadranka, "Prevalence of causative agents of subclinical mastitis in dairy cow" in Second International Symposium of Veterinary Medicine (ISVM2016) (2016):159-159.