Simultaneous coinfections with West Nile virus and Usutu virus in Culex pipiens and Aedes vexans mosquitoes
Аутори
Körsten, Christin![](/themes/Miragereponivs/images/orcid.png)
AL-Hosary, Amira A.
Holicki, Cora M.
![](/themes/Miragereponivs/images/orcid.png)
Schäfer, Mandy
Tews, Birke A.
Vasić, Ana
![](/themes/Miragereponivs/images/orcid.png)
Ziegler, Ute
![](/themes/Miragereponivs/images/orcid.png)
Groschup, Martin H.
![](/themes/Miragereponivs/images/orcid.png)
Silaghi, Cornelia
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The mosquito-borne zoonotic flaviviruses West Nile virus (WNV) and Usutu virus (USUV) are endemic in many European countries and emerged in Germany in recent years. Due to the increasing overlap of their distribution areas and their similar epidemiology, coinfections of WNV and USUV are possible. Indeed, coinfections in vertebrate hosts as a rare event have already been reported from some countries including Germany. However, it is largely unknown whether and to what extent coinfections could affect the vector competence of mosquitoes for WNV and USUV. For this purpose, the mosquito species Culex pipiens biotype pipiens, Culex pipiens biotype molestus, and Aedes vexans were orally infected in mono- and simultaneous coinfections with German strains of WNV and USUV. Mosquitoes were incubated for 14 days at 26°C, 85% relative humidity, and a 16 : 8 light-dark photocycle, before they were dissected and forced to salivate. The results showed a decrease in USUV susceptibility in Culex pipien...s biotype pipiens, an increase in USUV susceptibility in Aedes vexans, and no obvious interaction between both viruses in Culex pipiens biotype molestus. Vector competence for WNV appeared to be unaffected by a simultaneous occurrence of USUV in all tested mosquito species. Coinfections with both viruses were only found in Culex mosquitoes, and cotransmission of WNV and USUV was observed in Culex pipiens biotype molestus. Overall, our results show that viral interactions between WNV and USUV vary between mosquito species, and that the interaction mainly occurs during infection and replication in the mosquito midgut. The results of this study confirm that to fully understand the interaction between WNV and USUV, studies with various mosquito species are necessary. In addition, we found that even mosquito species with a low susceptibility to both viruses, such as Ae. vexans, can play a role in their transmission in areas with cocirculation.
Кључне речи:
West Nile virus / Usutu virus / coinfections / Culex pipiens / Aedes vexansИзвор:
Transboundary and Emerging Diseases, 2023, 6305484-Издавач:
- Wiley
Институција/група
Naučni institut za veterinarstvo SrbijeTY - JOUR AU - Körsten, Christin AU - AL-Hosary, Amira A. AU - Holicki, Cora M. AU - Schäfer, Mandy AU - Tews, Birke A. AU - Vasić, Ana AU - Ziegler, Ute AU - Groschup, Martin H. AU - Silaghi, Cornelia PY - 2023 UR - https://reponivs.nivs.rs/handle/123456789/571 AB - The mosquito-borne zoonotic flaviviruses West Nile virus (WNV) and Usutu virus (USUV) are endemic in many European countries and emerged in Germany in recent years. Due to the increasing overlap of their distribution areas and their similar epidemiology, coinfections of WNV and USUV are possible. Indeed, coinfections in vertebrate hosts as a rare event have already been reported from some countries including Germany. However, it is largely unknown whether and to what extent coinfections could affect the vector competence of mosquitoes for WNV and USUV. For this purpose, the mosquito species Culex pipiens biotype pipiens, Culex pipiens biotype molestus, and Aedes vexans were orally infected in mono- and simultaneous coinfections with German strains of WNV and USUV. Mosquitoes were incubated for 14 days at 26°C, 85% relative humidity, and a 16 : 8 light-dark photocycle, before they were dissected and forced to salivate. The results showed a decrease in USUV susceptibility in Culex pipiens biotype pipiens, an increase in USUV susceptibility in Aedes vexans, and no obvious interaction between both viruses in Culex pipiens biotype molestus. Vector competence for WNV appeared to be unaffected by a simultaneous occurrence of USUV in all tested mosquito species. Coinfections with both viruses were only found in Culex mosquitoes, and cotransmission of WNV and USUV was observed in Culex pipiens biotype molestus. Overall, our results show that viral interactions between WNV and USUV vary between mosquito species, and that the interaction mainly occurs during infection and replication in the mosquito midgut. The results of this study confirm that to fully understand the interaction between WNV and USUV, studies with various mosquito species are necessary. In addition, we found that even mosquito species with a low susceptibility to both viruses, such as Ae. vexans, can play a role in their transmission in areas with cocirculation. PB - Wiley T2 - Transboundary and Emerging Diseases T1 - Simultaneous coinfections with West Nile virus and Usutu virus in Culex pipiens and Aedes vexans mosquitoes SP - 6305484 DO - 10.1155/2023/6305484 ER -
@article{ author = "Körsten, Christin and AL-Hosary, Amira A. and Holicki, Cora M. and Schäfer, Mandy and Tews, Birke A. and Vasić, Ana and Ziegler, Ute and Groschup, Martin H. and Silaghi, Cornelia", year = "2023", abstract = "The mosquito-borne zoonotic flaviviruses West Nile virus (WNV) and Usutu virus (USUV) are endemic in many European countries and emerged in Germany in recent years. Due to the increasing overlap of their distribution areas and their similar epidemiology, coinfections of WNV and USUV are possible. Indeed, coinfections in vertebrate hosts as a rare event have already been reported from some countries including Germany. However, it is largely unknown whether and to what extent coinfections could affect the vector competence of mosquitoes for WNV and USUV. For this purpose, the mosquito species Culex pipiens biotype pipiens, Culex pipiens biotype molestus, and Aedes vexans were orally infected in mono- and simultaneous coinfections with German strains of WNV and USUV. Mosquitoes were incubated for 14 days at 26°C, 85% relative humidity, and a 16 : 8 light-dark photocycle, before they were dissected and forced to salivate. The results showed a decrease in USUV susceptibility in Culex pipiens biotype pipiens, an increase in USUV susceptibility in Aedes vexans, and no obvious interaction between both viruses in Culex pipiens biotype molestus. Vector competence for WNV appeared to be unaffected by a simultaneous occurrence of USUV in all tested mosquito species. Coinfections with both viruses were only found in Culex mosquitoes, and cotransmission of WNV and USUV was observed in Culex pipiens biotype molestus. Overall, our results show that viral interactions between WNV and USUV vary between mosquito species, and that the interaction mainly occurs during infection and replication in the mosquito midgut. The results of this study confirm that to fully understand the interaction between WNV and USUV, studies with various mosquito species are necessary. In addition, we found that even mosquito species with a low susceptibility to both viruses, such as Ae. vexans, can play a role in their transmission in areas with cocirculation.", publisher = "Wiley", journal = "Transboundary and Emerging Diseases", title = "Simultaneous coinfections with West Nile virus and Usutu virus in Culex pipiens and Aedes vexans mosquitoes", pages = "6305484", doi = "10.1155/2023/6305484" }
Körsten, C., AL-Hosary, A. A., Holicki, C. M., Schäfer, M., Tews, B. A., Vasić, A., Ziegler, U., Groschup, M. H.,& Silaghi, C.. (2023). Simultaneous coinfections with West Nile virus and Usutu virus in Culex pipiens and Aedes vexans mosquitoes. in Transboundary and Emerging Diseases Wiley., 6305484. https://doi.org/10.1155/2023/6305484
Körsten C, AL-Hosary AA, Holicki CM, Schäfer M, Tews BA, Vasić A, Ziegler U, Groschup MH, Silaghi C. Simultaneous coinfections with West Nile virus and Usutu virus in Culex pipiens and Aedes vexans mosquitoes. in Transboundary and Emerging Diseases. 2023;:6305484. doi:10.1155/2023/6305484 .
Körsten, Christin, AL-Hosary, Amira A., Holicki, Cora M., Schäfer, Mandy, Tews, Birke A., Vasić, Ana, Ziegler, Ute, Groschup, Martin H., Silaghi, Cornelia, "Simultaneous coinfections with West Nile virus and Usutu virus in Culex pipiens and Aedes vexans mosquitoes" in Transboundary and Emerging Diseases (2023):6305484, https://doi.org/10.1155/2023/6305484 . .