Assessment of different diagnostic methods for the identification of subclinical endometritis in dairy cows with pathological puerperium and their reliability to conceive
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2021
Аутори
Bajagić, BojanaMrkun, Janko
Kirovski, Danijela
Savić, Đorđe
Budimir, Drazenko
Maletić, Jelena
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Maletić, Milan
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Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The aim of study was to evaluate different methods for subclinical endometritis (SCE) diagnoses and their accuracy to predict pregnancy. The study was performed on 51 postpartum cross breed dairy cows with pathological puerperium. Artificial insemination (AI) was done in spontaneous estrus. Endometrium samples were taken by cytotape (CT), cervical mucus (CM) samples by Metricheck and smears for crystallization degree were obtained at AI day. Blood serum samples for progesterone (P4) concentration determination were taken at AI day and 17 days later. Cows were examined by ultrasound 35 days after AI to confirm pregnancy. Percent (%) of polymorphnuclear leukocytes (PMNs) taken by CT were expressed by ROC curve (with cut-off point set at 1%). Out of the total of 51 cows involved in the study, 13.73% true positive and 41.17% true negative cows were found (54.90%). The content of CM sampled with Metricheck was altered in 11 cows (21.57%), and one of them was pregnant (1.96%). An average mac...roscopic CM value for pregnant cows was 0.06 +/- 0.24 vs. 0.39 +/- 0.79 for non-pregnant (p LT 0.05). An average value of CM crystallization in pregnant cows was 2.50 +/- 1.04 vs. 1.85 +/- 1.15 in non-pregnant (p LT 0.05). P4 concentration was not statistically different at AI day, but was statistically significantly higher 17 days later in pregnant cows (p = 0.002). CT alterations may be a reliable diagnostic tool for SCE diagnosis, while macroscopic CM alterations may be used for CE detection. P4 determination is beneficial in pregnancy diagnosis while CM crystallization degree is helpful in determination of best time for AI.
Кључне речи:
subclinical endometritis / pregnancy / Metricheck / cytotape / cervical mucusИзвор:
Acta Veterinaria-Beograd, 2021, 71, 4, 462-476Издавач:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Финансирање / пројекти:
- Ministry of Education, Science and Technological Development of the Republic of Serbia [451-03-9/2021-14]
DOI: 10.2478/acve-2021-0038
ISSN: 0567-8315
WoS: 000744142000007
Scopus: 2-s2.0-85123706709
Институција/група
Naučni institut za veterinarstvo SrbijeTY - JOUR AU - Bajagić, Bojana AU - Mrkun, Janko AU - Kirovski, Danijela AU - Savić, Đorđe AU - Budimir, Drazenko AU - Maletić, Jelena AU - Maletić, Milan PY - 2021 UR - https://reponivs.nivs.rs/handle/123456789/460 AB - The aim of study was to evaluate different methods for subclinical endometritis (SCE) diagnoses and their accuracy to predict pregnancy. The study was performed on 51 postpartum cross breed dairy cows with pathological puerperium. Artificial insemination (AI) was done in spontaneous estrus. Endometrium samples were taken by cytotape (CT), cervical mucus (CM) samples by Metricheck and smears for crystallization degree were obtained at AI day. Blood serum samples for progesterone (P4) concentration determination were taken at AI day and 17 days later. Cows were examined by ultrasound 35 days after AI to confirm pregnancy. Percent (%) of polymorphnuclear leukocytes (PMNs) taken by CT were expressed by ROC curve (with cut-off point set at 1%). Out of the total of 51 cows involved in the study, 13.73% true positive and 41.17% true negative cows were found (54.90%). The content of CM sampled with Metricheck was altered in 11 cows (21.57%), and one of them was pregnant (1.96%). An average macroscopic CM value for pregnant cows was 0.06 +/- 0.24 vs. 0.39 +/- 0.79 for non-pregnant (p LT 0.05). An average value of CM crystallization in pregnant cows was 2.50 +/- 1.04 vs. 1.85 +/- 1.15 in non-pregnant (p LT 0.05). P4 concentration was not statistically different at AI day, but was statistically significantly higher 17 days later in pregnant cows (p = 0.002). CT alterations may be a reliable diagnostic tool for SCE diagnosis, while macroscopic CM alterations may be used for CE detection. P4 determination is beneficial in pregnancy diagnosis while CM crystallization degree is helpful in determination of best time for AI. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - Assessment of different diagnostic methods for the identification of subclinical endometritis in dairy cows with pathological puerperium and their reliability to conceive EP - 476 IS - 4 SP - 462 VL - 71 DO - 10.2478/acve-2021-0038 UR - conv_579 ER -
@article{ author = "Bajagić, Bojana and Mrkun, Janko and Kirovski, Danijela and Savić, Đorđe and Budimir, Drazenko and Maletić, Jelena and Maletić, Milan", year = "2021", abstract = "The aim of study was to evaluate different methods for subclinical endometritis (SCE) diagnoses and their accuracy to predict pregnancy. The study was performed on 51 postpartum cross breed dairy cows with pathological puerperium. Artificial insemination (AI) was done in spontaneous estrus. Endometrium samples were taken by cytotape (CT), cervical mucus (CM) samples by Metricheck and smears for crystallization degree were obtained at AI day. Blood serum samples for progesterone (P4) concentration determination were taken at AI day and 17 days later. Cows were examined by ultrasound 35 days after AI to confirm pregnancy. Percent (%) of polymorphnuclear leukocytes (PMNs) taken by CT were expressed by ROC curve (with cut-off point set at 1%). Out of the total of 51 cows involved in the study, 13.73% true positive and 41.17% true negative cows were found (54.90%). The content of CM sampled with Metricheck was altered in 11 cows (21.57%), and one of them was pregnant (1.96%). An average macroscopic CM value for pregnant cows was 0.06 +/- 0.24 vs. 0.39 +/- 0.79 for non-pregnant (p LT 0.05). An average value of CM crystallization in pregnant cows was 2.50 +/- 1.04 vs. 1.85 +/- 1.15 in non-pregnant (p LT 0.05). P4 concentration was not statistically different at AI day, but was statistically significantly higher 17 days later in pregnant cows (p = 0.002). CT alterations may be a reliable diagnostic tool for SCE diagnosis, while macroscopic CM alterations may be used for CE detection. P4 determination is beneficial in pregnancy diagnosis while CM crystallization degree is helpful in determination of best time for AI.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "Assessment of different diagnostic methods for the identification of subclinical endometritis in dairy cows with pathological puerperium and their reliability to conceive", pages = "476-462", number = "4", volume = "71", doi = "10.2478/acve-2021-0038", url = "conv_579" }
Bajagić, B., Mrkun, J., Kirovski, D., Savić, Đ., Budimir, D., Maletić, J.,& Maletić, M.. (2021). Assessment of different diagnostic methods for the identification of subclinical endometritis in dairy cows with pathological puerperium and their reliability to conceive. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 71(4), 462-476. https://doi.org/10.2478/acve-2021-0038 conv_579
Bajagić B, Mrkun J, Kirovski D, Savić Đ, Budimir D, Maletić J, Maletić M. Assessment of different diagnostic methods for the identification of subclinical endometritis in dairy cows with pathological puerperium and their reliability to conceive. in Acta Veterinaria-Beograd. 2021;71(4):462-476. doi:10.2478/acve-2021-0038 conv_579 .
Bajagić, Bojana, Mrkun, Janko, Kirovski, Danijela, Savić, Đorđe, Budimir, Drazenko, Maletić, Jelena, Maletić, Milan, "Assessment of different diagnostic methods for the identification of subclinical endometritis in dairy cows with pathological puerperium and their reliability to conceive" in Acta Veterinaria-Beograd, 71, no. 4 (2021):462-476, https://doi.org/10.2478/acve-2021-0038 ., conv_579 .