Comparison of vector competence of Aedes vexans Green River and Culex pipiens biotype pipiens for West Nile virus lineages 1 and 2
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2020
Аутори
Woehnke, ElisabethVasić, Ana
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Raileanu, Cristian
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Holicki, Cora M.
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Tews, Birke Andrea
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Silaghi, Cornelia
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
West Nile virus (WNV), a zoonotic arbovirus, has recently established an autochthonous transmission cycle in Germany. In dead-end hosts like humans and horses the WNV infection may cause severe symptoms in the central nervous system. In nature, WNV is maintained in an enzootic transmission cycle between birds and ornithophilic mosquitoes. Bridge vector species, such as members of the Culex pipiens complex and Aedes spp., also widely distributed in Germany, might transmit WNV to other vertebrate host species. This study determined and compared the vector competence of field-collected northern-German Cx. pipiens biotype pipiens and laboratory-reared Ae. vexans Green River (GR) for WNV lineage 1 (strain: Magpie/Italy/203204) and WNV lineage 2 (strain: "Austria") under temperatures typical for northern Germany in spring/summer and autumn. For assessment of vector competence, 7- to 14-day-old female mosquitoes were offered a WNV containing blood meal via Hemotek membrane feeding system or c...otton-stick feeding. After incubation at 18 degrees C respectively 24 degrees C for 14 days engorged female mosquitoes were salivated and dissected for determination of infection, dissemination and transmission rates by reverse transcriptase quantitative real-time PCR (RT-qPCR). Both Ae. vexans GR and Cx. pipiens biotype pipiens were infected with both tested WNV strains and tested 14 days post-inoculation. Disseminated infections were detected only in Ae. vexans GR incubated at 18 degrees C and in Cx. pipiens pipiens incubated at 24 degrees C after infection with WNV lineage 1. Transmission of WNV lineage 1 was detected in Cx. pipiens pipiens incubated at 24 degrees C. These results indicate that Cx. pipiens pipiens from Northern Germany may be involved in the transmission of WNV, also to dead-end hosts like humans and horses.
Кључне речи:
West Nile virus / vector competence / Culex pipiens pipiens / Aedes vexansИзвор:
Zoonoses and Public Health, 2020, 67, 4, 416-424Издавач:
- Wiley, Hoboken
DOI: 10.1111/zph.12700
ISSN: 1863-1959
PubMed: 32162489
WoS: 000535710600009
Scopus: 2-s2.0-85081745990
Институција/група
Naučni institut za veterinarstvo SrbijeTY - JOUR AU - Woehnke, Elisabeth AU - Vasić, Ana AU - Raileanu, Cristian AU - Holicki, Cora M. AU - Tews, Birke Andrea AU - Silaghi, Cornelia PY - 2020 UR - https://reponivs.nivs.rs/handle/123456789/424 AB - West Nile virus (WNV), a zoonotic arbovirus, has recently established an autochthonous transmission cycle in Germany. In dead-end hosts like humans and horses the WNV infection may cause severe symptoms in the central nervous system. In nature, WNV is maintained in an enzootic transmission cycle between birds and ornithophilic mosquitoes. Bridge vector species, such as members of the Culex pipiens complex and Aedes spp., also widely distributed in Germany, might transmit WNV to other vertebrate host species. This study determined and compared the vector competence of field-collected northern-German Cx. pipiens biotype pipiens and laboratory-reared Ae. vexans Green River (GR) for WNV lineage 1 (strain: Magpie/Italy/203204) and WNV lineage 2 (strain: "Austria") under temperatures typical for northern Germany in spring/summer and autumn. For assessment of vector competence, 7- to 14-day-old female mosquitoes were offered a WNV containing blood meal via Hemotek membrane feeding system or cotton-stick feeding. After incubation at 18 degrees C respectively 24 degrees C for 14 days engorged female mosquitoes were salivated and dissected for determination of infection, dissemination and transmission rates by reverse transcriptase quantitative real-time PCR (RT-qPCR). Both Ae. vexans GR and Cx. pipiens biotype pipiens were infected with both tested WNV strains and tested 14 days post-inoculation. Disseminated infections were detected only in Ae. vexans GR incubated at 18 degrees C and in Cx. pipiens pipiens incubated at 24 degrees C after infection with WNV lineage 1. Transmission of WNV lineage 1 was detected in Cx. pipiens pipiens incubated at 24 degrees C. These results indicate that Cx. pipiens pipiens from Northern Germany may be involved in the transmission of WNV, also to dead-end hosts like humans and horses. PB - Wiley, Hoboken T2 - Zoonoses and Public Health T1 - Comparison of vector competence of Aedes vexans Green River and Culex pipiens biotype pipiens for West Nile virus lineages 1 and 2 EP - 424 IS - 4 SP - 416 VL - 67 DO - 10.1111/zph.12700 UR - conv_545 ER -
@article{ author = "Woehnke, Elisabeth and Vasić, Ana and Raileanu, Cristian and Holicki, Cora M. and Tews, Birke Andrea and Silaghi, Cornelia", year = "2020", abstract = "West Nile virus (WNV), a zoonotic arbovirus, has recently established an autochthonous transmission cycle in Germany. In dead-end hosts like humans and horses the WNV infection may cause severe symptoms in the central nervous system. In nature, WNV is maintained in an enzootic transmission cycle between birds and ornithophilic mosquitoes. Bridge vector species, such as members of the Culex pipiens complex and Aedes spp., also widely distributed in Germany, might transmit WNV to other vertebrate host species. This study determined and compared the vector competence of field-collected northern-German Cx. pipiens biotype pipiens and laboratory-reared Ae. vexans Green River (GR) for WNV lineage 1 (strain: Magpie/Italy/203204) and WNV lineage 2 (strain: "Austria") under temperatures typical for northern Germany in spring/summer and autumn. For assessment of vector competence, 7- to 14-day-old female mosquitoes were offered a WNV containing blood meal via Hemotek membrane feeding system or cotton-stick feeding. After incubation at 18 degrees C respectively 24 degrees C for 14 days engorged female mosquitoes were salivated and dissected for determination of infection, dissemination and transmission rates by reverse transcriptase quantitative real-time PCR (RT-qPCR). Both Ae. vexans GR and Cx. pipiens biotype pipiens were infected with both tested WNV strains and tested 14 days post-inoculation. Disseminated infections were detected only in Ae. vexans GR incubated at 18 degrees C and in Cx. pipiens pipiens incubated at 24 degrees C after infection with WNV lineage 1. Transmission of WNV lineage 1 was detected in Cx. pipiens pipiens incubated at 24 degrees C. These results indicate that Cx. pipiens pipiens from Northern Germany may be involved in the transmission of WNV, also to dead-end hosts like humans and horses.", publisher = "Wiley, Hoboken", journal = "Zoonoses and Public Health", title = "Comparison of vector competence of Aedes vexans Green River and Culex pipiens biotype pipiens for West Nile virus lineages 1 and 2", pages = "424-416", number = "4", volume = "67", doi = "10.1111/zph.12700", url = "conv_545" }
Woehnke, E., Vasić, A., Raileanu, C., Holicki, C. M., Tews, B. A.,& Silaghi, C.. (2020). Comparison of vector competence of Aedes vexans Green River and Culex pipiens biotype pipiens for West Nile virus lineages 1 and 2. in Zoonoses and Public Health Wiley, Hoboken., 67(4), 416-424. https://doi.org/10.1111/zph.12700 conv_545
Woehnke E, Vasić A, Raileanu C, Holicki CM, Tews BA, Silaghi C. Comparison of vector competence of Aedes vexans Green River and Culex pipiens biotype pipiens for West Nile virus lineages 1 and 2. in Zoonoses and Public Health. 2020;67(4):416-424. doi:10.1111/zph.12700 conv_545 .
Woehnke, Elisabeth, Vasić, Ana, Raileanu, Cristian, Holicki, Cora M., Tews, Birke Andrea, Silaghi, Cornelia, "Comparison of vector competence of Aedes vexans Green River and Culex pipiens biotype pipiens for West Nile virus lineages 1 and 2" in Zoonoses and Public Health, 67, no. 4 (2020):416-424, https://doi.org/10.1111/zph.12700 ., conv_545 .