Comparison of performances between three commercial elisa kits for detection of antibodies against porcine reproductive and respiratory syndrome virus (PRRSV) in swine sera samples
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2020
Аутори
Zurovac-Sapundžić, Zorana![](/themes/Miragereponivs/images/orcid.png)
Ninković, Milan
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Milovanović, Bojan
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Glišić, Dimitrije
![](/themes/Miragereponivs/images/orcid.png)
Milićević, Vesna
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Kureljušić, Branislav
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Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Porcine Reproductive and Respiratory Syndrome (PRRS) is one of the most economically important diseases in pigs, worldwide. In the USA alone, the total cost to the swine industry has been estimated at $664 million per year. Therefore, the continuous and reliable monitoring of the PRRS status of a pig herd is required in order to prevent and reduce the costs caused by this infection. Nowadays, commonly used methods for laboratory diagnosis of PRRS infection are serological (ELISA) and molecular (PCR) ones. This study aims to assess the sensitivity and specificity of three different commercially available ELISA kits for detection of antibodies against PRRSV (IDEXX PRRS X3 Ab Test (IDEXX, USA), INgezim PRRS Universal (Ingenasa, Spain), and Pigtype PRRSV Ab (Qiagen, Germany)) using 91 blood serum samples collected from pigs in Serbia. Our study showed a certain level of differences in specificity and sensitivity between three commercially available ELISA kits. However, IDEXX ELISA proved t...o be a more reliable kit for detecting antibodies against PRRSV with sensitivity of 97.4% and specificity of 98.1%, compared to INgezim and Qiagen kits specificity of 92.5% and 83%, respectively, and sensitivity of 94.7% for both kits. In order to achieve maximal reliability of the obtained results, ELISA diagnostic protocol for laboratory diagnosis of PRRS infection should be complemented with additional tests such as PCR and virus neutralization test.
Кључне речи:
specificity / sensitivity / PRRS / ELISAИзвор:
Archives of Veterinary Medicine, 2020, 13, 2, 77-86Издавач:
- Novi Sad : Naučni institut za veterinarstvo "Novi Sad"
Институција/група
Naučni institut za veterinarstvo SrbijeTY - JOUR AU - Zurovac-Sapundžić, Zorana AU - Ninković, Milan AU - Milovanović, Bojan AU - Glišić, Dimitrije AU - Milićević, Vesna AU - Kureljušić, Branislav PY - 2020 UR - https://reponivs.nivs.rs/handle/123456789/410 AB - Porcine Reproductive and Respiratory Syndrome (PRRS) is one of the most economically important diseases in pigs, worldwide. In the USA alone, the total cost to the swine industry has been estimated at $664 million per year. Therefore, the continuous and reliable monitoring of the PRRS status of a pig herd is required in order to prevent and reduce the costs caused by this infection. Nowadays, commonly used methods for laboratory diagnosis of PRRS infection are serological (ELISA) and molecular (PCR) ones. This study aims to assess the sensitivity and specificity of three different commercially available ELISA kits for detection of antibodies against PRRSV (IDEXX PRRS X3 Ab Test (IDEXX, USA), INgezim PRRS Universal (Ingenasa, Spain), and Pigtype PRRSV Ab (Qiagen, Germany)) using 91 blood serum samples collected from pigs in Serbia. Our study showed a certain level of differences in specificity and sensitivity between three commercially available ELISA kits. However, IDEXX ELISA proved to be a more reliable kit for detecting antibodies against PRRSV with sensitivity of 97.4% and specificity of 98.1%, compared to INgezim and Qiagen kits specificity of 92.5% and 83%, respectively, and sensitivity of 94.7% for both kits. In order to achieve maximal reliability of the obtained results, ELISA diagnostic protocol for laboratory diagnosis of PRRS infection should be complemented with additional tests such as PCR and virus neutralization test. PB - Novi Sad : Naučni institut za veterinarstvo "Novi Sad" T2 - Archives of Veterinary Medicine T1 - Comparison of performances between three commercial elisa kits for detection of antibodies against porcine reproductive and respiratory syndrome virus (PRRSV) in swine sera samples EP - 86 IS - 2 SP - 77 VL - 13 DO - 10.46784/eavm.v13i2.252 UR - conv_631 ER -
@article{ author = "Zurovac-Sapundžić, Zorana and Ninković, Milan and Milovanović, Bojan and Glišić, Dimitrije and Milićević, Vesna and Kureljušić, Branislav", year = "2020", abstract = "Porcine Reproductive and Respiratory Syndrome (PRRS) is one of the most economically important diseases in pigs, worldwide. In the USA alone, the total cost to the swine industry has been estimated at $664 million per year. Therefore, the continuous and reliable monitoring of the PRRS status of a pig herd is required in order to prevent and reduce the costs caused by this infection. Nowadays, commonly used methods for laboratory diagnosis of PRRS infection are serological (ELISA) and molecular (PCR) ones. This study aims to assess the sensitivity and specificity of three different commercially available ELISA kits for detection of antibodies against PRRSV (IDEXX PRRS X3 Ab Test (IDEXX, USA), INgezim PRRS Universal (Ingenasa, Spain), and Pigtype PRRSV Ab (Qiagen, Germany)) using 91 blood serum samples collected from pigs in Serbia. Our study showed a certain level of differences in specificity and sensitivity between three commercially available ELISA kits. However, IDEXX ELISA proved to be a more reliable kit for detecting antibodies against PRRSV with sensitivity of 97.4% and specificity of 98.1%, compared to INgezim and Qiagen kits specificity of 92.5% and 83%, respectively, and sensitivity of 94.7% for both kits. In order to achieve maximal reliability of the obtained results, ELISA diagnostic protocol for laboratory diagnosis of PRRS infection should be complemented with additional tests such as PCR and virus neutralization test.", publisher = "Novi Sad : Naučni institut za veterinarstvo "Novi Sad"", journal = "Archives of Veterinary Medicine", title = "Comparison of performances between three commercial elisa kits for detection of antibodies against porcine reproductive and respiratory syndrome virus (PRRSV) in swine sera samples", pages = "86-77", number = "2", volume = "13", doi = "10.46784/eavm.v13i2.252", url = "conv_631" }
Zurovac-Sapundžić, Z., Ninković, M., Milovanović, B., Glišić, D., Milićević, V.,& Kureljušić, B.. (2020). Comparison of performances between three commercial elisa kits for detection of antibodies against porcine reproductive and respiratory syndrome virus (PRRSV) in swine sera samples. in Archives of Veterinary Medicine Novi Sad : Naučni institut za veterinarstvo "Novi Sad"., 13(2), 77-86. https://doi.org/10.46784/eavm.v13i2.252 conv_631
Zurovac-Sapundžić Z, Ninković M, Milovanović B, Glišić D, Milićević V, Kureljušić B. Comparison of performances between three commercial elisa kits for detection of antibodies against porcine reproductive and respiratory syndrome virus (PRRSV) in swine sera samples. in Archives of Veterinary Medicine. 2020;13(2):77-86. doi:10.46784/eavm.v13i2.252 conv_631 .
Zurovac-Sapundžić, Zorana, Ninković, Milan, Milovanović, Bojan, Glišić, Dimitrije, Milićević, Vesna, Kureljušić, Branislav, "Comparison of performances between three commercial elisa kits for detection of antibodies against porcine reproductive and respiratory syndrome virus (PRRSV) in swine sera samples" in Archives of Veterinary Medicine, 13, no. 2 (2020):77-86, https://doi.org/10.46784/eavm.v13i2.252 ., conv_631 .