Molecular detection of black queen cell virus and Kashmir bee virus in honey
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2018
Аутори
Milićević, Vesna![](/themes/Miragereponivs/images/orcid.png)
Radojičić, Sonja
![](/themes/Miragereponivs/images/orcid.png)
Kureljušić, Jasna
![](/themes/Miragereponivs/images/orcid.png)
Šekler, Milanko
Nešić, Ksenija
![](/themes/Miragereponivs/images/orcid.png)
Veljović, Ljubiša
![](/themes/Miragereponivs/images/orcid.png)
Maksimović Zorić, Jelena
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Radosavljević, Vladimir
![](/themes/Miragereponivs/images/orcid.png)
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Considering the intensive trading nowadays, the honey from the local market was tested for the presence of the six most common bee viruses. To prove the suitability of honey as a sample for the bee viruses detection, the set of different sample types taken directly from the hives we comparatively tested. The study included 30 samples of domestic and 5 samples of imported honey. Additionally, we tested 40 sets of samples including live bees, dead bees, and the honey taken from four apiaries for the evaluation of honey suitability for the virus detection, Two out of the six most common bee viruses were detected in the samples of honey from the market. Black queen cell virus (BQCV) genome was found in 24 domestic honey samples and Kashmir bee virus (KBV) genome was detected in one sample of imported honey. The nucleotide sequences of 24 BQCV isolates showed the highest identity (86.4%) with strains from Europe at the polyprotein gene, whilst the Serbian isolates between each other showed ...98.5% similarity. By comparative testing of the different type of samples, in three out of four apiaries BQCV genome was detected in both bees and honey. Evaluating the suitability of honey for the detection of the viral disease by simultaneous testing of live, dead bees, and honey from the same hive, it was shown that the honey can be successfully used for the detection of BQCV. Since, as of yet, there has been no evidence of KBV circulation in Serbia, after its detection in imported honey, there is a substantial risk of its introduction and consequently the need for its surveillance. Therefore, the programs of bee diseases screening should be included in the regular control procedures for the international trade. In addition to this benefit, honey gives an opportunity to beekeepers for continuous monitoring of bees' health status.
Кључне речи:
RT-PCR / Phylogenetic analysis / Honey / Honey bee virusesИзвор:
AMB Express, 2018, 8Издавач:
- Springeropen, London
Финансирање / пројекти:
- Institute of Veterinary Medicine of Serbia
DOI: 10.1186/s13568-018-0655-7
ISSN: 2191-0855
PubMed: 30088183
WoS: 000441119300001
Scopus: 2-s2.0-85051280179
Институција/група
Naučni institut za veterinarstvo SrbijeTY - JOUR AU - Milićević, Vesna AU - Radojičić, Sonja AU - Kureljušić, Jasna AU - Šekler, Milanko AU - Nešić, Ksenija AU - Veljović, Ljubiša AU - Maksimović Zorić, Jelena AU - Radosavljević, Vladimir PY - 2018 UR - https://reponivs.nivs.rs/handle/123456789/355 AB - Considering the intensive trading nowadays, the honey from the local market was tested for the presence of the six most common bee viruses. To prove the suitability of honey as a sample for the bee viruses detection, the set of different sample types taken directly from the hives we comparatively tested. The study included 30 samples of domestic and 5 samples of imported honey. Additionally, we tested 40 sets of samples including live bees, dead bees, and the honey taken from four apiaries for the evaluation of honey suitability for the virus detection, Two out of the six most common bee viruses were detected in the samples of honey from the market. Black queen cell virus (BQCV) genome was found in 24 domestic honey samples and Kashmir bee virus (KBV) genome was detected in one sample of imported honey. The nucleotide sequences of 24 BQCV isolates showed the highest identity (86.4%) with strains from Europe at the polyprotein gene, whilst the Serbian isolates between each other showed 98.5% similarity. By comparative testing of the different type of samples, in three out of four apiaries BQCV genome was detected in both bees and honey. Evaluating the suitability of honey for the detection of the viral disease by simultaneous testing of live, dead bees, and honey from the same hive, it was shown that the honey can be successfully used for the detection of BQCV. Since, as of yet, there has been no evidence of KBV circulation in Serbia, after its detection in imported honey, there is a substantial risk of its introduction and consequently the need for its surveillance. Therefore, the programs of bee diseases screening should be included in the regular control procedures for the international trade. In addition to this benefit, honey gives an opportunity to beekeepers for continuous monitoring of bees' health status. PB - Springeropen, London T2 - AMB Express T1 - Molecular detection of black queen cell virus and Kashmir bee virus in honey VL - 8 DO - 10.1186/s13568-018-0655-7 UR - conv_513 ER -
@article{ author = "Milićević, Vesna and Radojičić, Sonja and Kureljušić, Jasna and Šekler, Milanko and Nešić, Ksenija and Veljović, Ljubiša and Maksimović Zorić, Jelena and Radosavljević, Vladimir", year = "2018", abstract = "Considering the intensive trading nowadays, the honey from the local market was tested for the presence of the six most common bee viruses. To prove the suitability of honey as a sample for the bee viruses detection, the set of different sample types taken directly from the hives we comparatively tested. The study included 30 samples of domestic and 5 samples of imported honey. Additionally, we tested 40 sets of samples including live bees, dead bees, and the honey taken from four apiaries for the evaluation of honey suitability for the virus detection, Two out of the six most common bee viruses were detected in the samples of honey from the market. Black queen cell virus (BQCV) genome was found in 24 domestic honey samples and Kashmir bee virus (KBV) genome was detected in one sample of imported honey. The nucleotide sequences of 24 BQCV isolates showed the highest identity (86.4%) with strains from Europe at the polyprotein gene, whilst the Serbian isolates between each other showed 98.5% similarity. By comparative testing of the different type of samples, in three out of four apiaries BQCV genome was detected in both bees and honey. Evaluating the suitability of honey for the detection of the viral disease by simultaneous testing of live, dead bees, and honey from the same hive, it was shown that the honey can be successfully used for the detection of BQCV. Since, as of yet, there has been no evidence of KBV circulation in Serbia, after its detection in imported honey, there is a substantial risk of its introduction and consequently the need for its surveillance. Therefore, the programs of bee diseases screening should be included in the regular control procedures for the international trade. In addition to this benefit, honey gives an opportunity to beekeepers for continuous monitoring of bees' health status.", publisher = "Springeropen, London", journal = "AMB Express", title = "Molecular detection of black queen cell virus and Kashmir bee virus in honey", volume = "8", doi = "10.1186/s13568-018-0655-7", url = "conv_513" }
Milićević, V., Radojičić, S., Kureljušić, J., Šekler, M., Nešić, K., Veljović, L., Maksimović Zorić, J.,& Radosavljević, V.. (2018). Molecular detection of black queen cell virus and Kashmir bee virus in honey. in AMB Express Springeropen, London., 8. https://doi.org/10.1186/s13568-018-0655-7 conv_513
Milićević V, Radojičić S, Kureljušić J, Šekler M, Nešić K, Veljović L, Maksimović Zorić J, Radosavljević V. Molecular detection of black queen cell virus and Kashmir bee virus in honey. in AMB Express. 2018;8. doi:10.1186/s13568-018-0655-7 conv_513 .
Milićević, Vesna, Radojičić, Sonja, Kureljušić, Jasna, Šekler, Milanko, Nešić, Ksenija, Veljović, Ljubiša, Maksimović Zorić, Jelena, Radosavljević, Vladimir, "Molecular detection of black queen cell virus and Kashmir bee virus in honey" in AMB Express, 8 (2018), https://doi.org/10.1186/s13568-018-0655-7 ., conv_513 .