Metode detekcije i tipizacije meticilin-rezistentnih Staphylococcus aureus izolovanih iz životinja
Metody obnaruženija i tipizacii meticillin-rezistentnyh Staphylococcus aureus vydelenyh iz životnyh
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2014
Аутори
Radosavljević, Vladimir![](/themes/Miragereponivs/images/orcid.png)
Žutić, Jadranka
![](/themes/Miragereponivs/images/orcid.png)
Pavlović, Ljiljana
Bošković, Tamara
Radanović, Oliver
![](/themes/Miragereponivs/images/orcid.png)
Žutić, Milenko
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
U radu je izvršena evaluacija metoda detekcije meticilin rezistentnog Staphylococcus aureus (MRSA) upotrebom dva molekularna i tri fenotipska testa u postupku ispitivanja 70 sojeva S. aureus izolovanih iz životinja. Skorašnji nalaz novog mecA homologa, mecALGA251, umanjuje značaj dokazivanja prisustva mecA gena kao potvrdne metode u identifikaciji meticilin rezistentnog Staphylococcus aureus. Iz ovog razloga je, pored multipleks PCR seta prajmera (16S rDNK, nuc, mecA) za detekciju mecA gena, korišćen i multipleks PCR set prajmera (spa, mecA, pvl, mecALGA251) za diferencijaciju mecALGA251 od mecA, sa istovremenom detekcjom lukF-PV i spa genskih fragmenata. Kod svih 70 ispitivanih izolata detektovano je prisustvo specifičnog 16S rDNK fragmenta i nuc gena koji kodira termostabilnu nukleazu S. aureus, dok je kod 5 od 70 izolata S. aureus pomoću dva multipleks PCR testa dokazano prisustvo mecA gena. U ispitivanim sojevima nije utvrđeno prisustvo mecC (mecALGA251) gena, niti Panton Valentin ...Leukocidin kodirajućeg gena. Primenom cefoksitin disk-difuzionog, lateks-aglutinacionog i dva multipleks PCR testa, dobijeni su identični rezultati u identifikaciji pet meticilin rezistentnih od 70 ukupno ispitanih sojeva S. aureus. U našem istraživanju utvrđena je potpuna korelacija između rezultata fenotipske i genotipske identifikacije meticilin rezistentnog S. aureus.
In this work there was evaluated the method of detection of methicillin resistant Staphylococcus aureus (MRSA) by using two molecular and three phenotypic tests in investigation procedure of 70 strains of S.aureus isolated from animals. Recent findings of the new mecA homologue, mecALGA251, minimise the significance of mecA gene presence detection as a confirmation method of methicillin resistant Staphylococcus aureus identification. For this reason, along with multiplex PCR set of primers (165rDNK, nuc, mecA) for detection mecA gene, there was also used multiplex PCR set of primers (spa, mecA, pvl, mecALGA251) for differentiation mecALGA251 from mecA, with simultaneous detection of luk-PV and spa gene fragments. In all 70 investigated isolates there was detected the presence of specific 16 SrDNK fragment and nuc gene which encodes a thermostable S. aureus nuclease, while in 5 out of 70 S. aureus isolates, there was proven mecA gene presence using two multiplex PCR tests. In the invest...igated strains there was determined neither mecC (mecALGA251)gene presence, nor Panton Valentine Leukocidin encoding gene. By application cefoxitin disk-diffusion, latex-agglutination and two multiplex PCR tests, the identical results in identification 5 methicillin resistant out of 70 investigated S. aureus strains were obtained. In our investigation there was determined a complete correlation between the results of phenotypic and genotypic identification of methicillin resistant S. aureus.
Кључне речи:
Staphylococcus aureus / multipleks PCR / MRSA / mecALGA251 / cefoksitin / Staphylococcus aureus / multiplex PCR / MRSA / mecALGA251 / cefoxitinИзвор:
Veterinarski glasnik, 2014, 68, 1-2, 89-99Издавач:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Финансирање / пројекти:
- Увођење и евалуација нове молекуларне методе за брзо откривање mecA гена стафилокока директно у брисевима узетим од људи, животиња и из њиховог окружења (RS-MESTD-Technological Development (TD or TR)-31079)
Институција/група
Naučni institut za veterinarstvo SrbijeTY - JOUR AU - Radosavljević, Vladimir AU - Žutić, Jadranka AU - Pavlović, Ljiljana AU - Bošković, Tamara AU - Radanović, Oliver AU - Žutić, Milenko PY - 2014 UR - https://reponivs.nivs.rs/handle/123456789/261 AB - U radu je izvršena evaluacija metoda detekcije meticilin rezistentnog Staphylococcus aureus (MRSA) upotrebom dva molekularna i tri fenotipska testa u postupku ispitivanja 70 sojeva S. aureus izolovanih iz životinja. Skorašnji nalaz novog mecA homologa, mecALGA251, umanjuje značaj dokazivanja prisustva mecA gena kao potvrdne metode u identifikaciji meticilin rezistentnog Staphylococcus aureus. Iz ovog razloga je, pored multipleks PCR seta prajmera (16S rDNK, nuc, mecA) za detekciju mecA gena, korišćen i multipleks PCR set prajmera (spa, mecA, pvl, mecALGA251) za diferencijaciju mecALGA251 od mecA, sa istovremenom detekcjom lukF-PV i spa genskih fragmenata. Kod svih 70 ispitivanih izolata detektovano je prisustvo specifičnog 16S rDNK fragmenta i nuc gena koji kodira termostabilnu nukleazu S. aureus, dok je kod 5 od 70 izolata S. aureus pomoću dva multipleks PCR testa dokazano prisustvo mecA gena. U ispitivanim sojevima nije utvrđeno prisustvo mecC (mecALGA251) gena, niti Panton Valentin Leukocidin kodirajućeg gena. Primenom cefoksitin disk-difuzionog, lateks-aglutinacionog i dva multipleks PCR testa, dobijeni su identični rezultati u identifikaciji pet meticilin rezistentnih od 70 ukupno ispitanih sojeva S. aureus. U našem istraživanju utvrđena je potpuna korelacija između rezultata fenotipske i genotipske identifikacije meticilin rezistentnog S. aureus. AB - In this work there was evaluated the method of detection of methicillin resistant Staphylococcus aureus (MRSA) by using two molecular and three phenotypic tests in investigation procedure of 70 strains of S.aureus isolated from animals. Recent findings of the new mecA homologue, mecALGA251, minimise the significance of mecA gene presence detection as a confirmation method of methicillin resistant Staphylococcus aureus identification. For this reason, along with multiplex PCR set of primers (165rDNK, nuc, mecA) for detection mecA gene, there was also used multiplex PCR set of primers (spa, mecA, pvl, mecALGA251) for differentiation mecALGA251 from mecA, with simultaneous detection of luk-PV and spa gene fragments. In all 70 investigated isolates there was detected the presence of specific 16 SrDNK fragment and nuc gene which encodes a thermostable S. aureus nuclease, while in 5 out of 70 S. aureus isolates, there was proven mecA gene presence using two multiplex PCR tests. In the investigated strains there was determined neither mecC (mecALGA251)gene presence, nor Panton Valentine Leukocidin encoding gene. By application cefoxitin disk-diffusion, latex-agglutination and two multiplex PCR tests, the identical results in identification 5 methicillin resistant out of 70 investigated S. aureus strains were obtained. In our investigation there was determined a complete correlation between the results of phenotypic and genotypic identification of methicillin resistant S. aureus. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Veterinarski glasnik T1 - Metode detekcije i tipizacije meticilin-rezistentnih Staphylococcus aureus izolovanih iz životinja T1 - Metody obnaruženija i tipizacii meticillin-rezistentnyh Staphylococcus aureus vydelenyh iz životnyh T1 - Methods of detection and typing of methicillin resistant Staphylococcus aureus isolated from animals EP - 99 IS - 1-2 SP - 89 VL - 68 DO - 10.2298/VETGL1402089R UR - conv_101 ER -
@article{ author = "Radosavljević, Vladimir and Žutić, Jadranka and Pavlović, Ljiljana and Bošković, Tamara and Radanović, Oliver and Žutić, Milenko", year = "2014", abstract = "U radu je izvršena evaluacija metoda detekcije meticilin rezistentnog Staphylococcus aureus (MRSA) upotrebom dva molekularna i tri fenotipska testa u postupku ispitivanja 70 sojeva S. aureus izolovanih iz životinja. Skorašnji nalaz novog mecA homologa, mecALGA251, umanjuje značaj dokazivanja prisustva mecA gena kao potvrdne metode u identifikaciji meticilin rezistentnog Staphylococcus aureus. Iz ovog razloga je, pored multipleks PCR seta prajmera (16S rDNK, nuc, mecA) za detekciju mecA gena, korišćen i multipleks PCR set prajmera (spa, mecA, pvl, mecALGA251) za diferencijaciju mecALGA251 od mecA, sa istovremenom detekcjom lukF-PV i spa genskih fragmenata. Kod svih 70 ispitivanih izolata detektovano je prisustvo specifičnog 16S rDNK fragmenta i nuc gena koji kodira termostabilnu nukleazu S. aureus, dok je kod 5 od 70 izolata S. aureus pomoću dva multipleks PCR testa dokazano prisustvo mecA gena. U ispitivanim sojevima nije utvrđeno prisustvo mecC (mecALGA251) gena, niti Panton Valentin Leukocidin kodirajućeg gena. Primenom cefoksitin disk-difuzionog, lateks-aglutinacionog i dva multipleks PCR testa, dobijeni su identični rezultati u identifikaciji pet meticilin rezistentnih od 70 ukupno ispitanih sojeva S. aureus. U našem istraživanju utvrđena je potpuna korelacija između rezultata fenotipske i genotipske identifikacije meticilin rezistentnog S. aureus., In this work there was evaluated the method of detection of methicillin resistant Staphylococcus aureus (MRSA) by using two molecular and three phenotypic tests in investigation procedure of 70 strains of S.aureus isolated from animals. Recent findings of the new mecA homologue, mecALGA251, minimise the significance of mecA gene presence detection as a confirmation method of methicillin resistant Staphylococcus aureus identification. For this reason, along with multiplex PCR set of primers (165rDNK, nuc, mecA) for detection mecA gene, there was also used multiplex PCR set of primers (spa, mecA, pvl, mecALGA251) for differentiation mecALGA251 from mecA, with simultaneous detection of luk-PV and spa gene fragments. In all 70 investigated isolates there was detected the presence of specific 16 SrDNK fragment and nuc gene which encodes a thermostable S. aureus nuclease, while in 5 out of 70 S. aureus isolates, there was proven mecA gene presence using two multiplex PCR tests. In the investigated strains there was determined neither mecC (mecALGA251)gene presence, nor Panton Valentine Leukocidin encoding gene. By application cefoxitin disk-diffusion, latex-agglutination and two multiplex PCR tests, the identical results in identification 5 methicillin resistant out of 70 investigated S. aureus strains were obtained. In our investigation there was determined a complete correlation between the results of phenotypic and genotypic identification of methicillin resistant S. aureus.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Veterinarski glasnik", title = "Metode detekcije i tipizacije meticilin-rezistentnih Staphylococcus aureus izolovanih iz životinja, Metody obnaruženija i tipizacii meticillin-rezistentnyh Staphylococcus aureus vydelenyh iz životnyh, Methods of detection and typing of methicillin resistant Staphylococcus aureus isolated from animals", pages = "99-89", number = "1-2", volume = "68", doi = "10.2298/VETGL1402089R", url = "conv_101" }
Radosavljević, V., Žutić, J., Pavlović, L., Bošković, T., Radanović, O.,& Žutić, M.. (2014). Metode detekcije i tipizacije meticilin-rezistentnih Staphylococcus aureus izolovanih iz životinja. in Veterinarski glasnik Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 68(1-2), 89-99. https://doi.org/10.2298/VETGL1402089R conv_101
Radosavljević V, Žutić J, Pavlović L, Bošković T, Radanović O, Žutić M. Metode detekcije i tipizacije meticilin-rezistentnih Staphylococcus aureus izolovanih iz životinja. in Veterinarski glasnik. 2014;68(1-2):89-99. doi:10.2298/VETGL1402089R conv_101 .
Radosavljević, Vladimir, Žutić, Jadranka, Pavlović, Ljiljana, Bošković, Tamara, Radanović, Oliver, Žutić, Milenko, "Metode detekcije i tipizacije meticilin-rezistentnih Staphylococcus aureus izolovanih iz životinja" in Veterinarski glasnik, 68, no. 1-2 (2014):89-99, https://doi.org/10.2298/VETGL1402089R ., conv_101 .
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