TY  - CONF
AU  - Maksimović Zorić, Jelena
AU  - Veljović, Ljubiša
AU  - Glišić, Dimitrije
AU  - Kureljušić, Branislav
AU  - Jezdimirović, Nemanja
AU  - Stanojević, Slobodan
AU  - Maletić, Jelena
AU  - Ninković, Milan
AU  - Aksić, Đorđe
AU  - Savić, Božidar
PY  - 2024
UR  - https://reponivs.nivs.rs/handle/123456789/951
AB  - Афричка куга свиња (АКС) је први пут потврђена на територији Републике
Србије у јулу 2019. године, код домаћих свиња на епизоотиолошком подручју
Града Београда. Епизоотиолошким увиђајем је установљено да је неодговорно
понашање људи довело до уноса вируса из околних земаља и ширења заразе. У
првом таласу регистрована су жаришта у три насељена места, а применом
законом прописаних мера, болест је стављена под контролу и одјављена
децембра 2019. године. Упркос поновном уносу вируса из околних земаља и
ширењу заразе у популацијама дивљих и домаћих свиња у североисточним,
централним, источним и јужним деловима земље, ситуација на територији
епизоотиолошког подручја Града Београда у периоду од 2020. до 2022. године
је била стабилна. Поновно избијање заразе десило се 9. јуна 2023. године када
је болест потврђена код угинуле дивље свиње пронађене у ловишту
"Панчевачки рит", на територији општине Палилула. Већ 12. јуна потврђен је и
први случај код екстензивно узгајаних домаћих свиња у насљеном месту
Крњача. Зараза је убрзо потврђена и код дивљих свиња суседног ловишта "Рит"
и домаћих свиња других насељених места општине Палилула (Бесни Фок,
Дунавац, Јабучки Рит, Ковилово, Падинска Скела, Борча, Овча, Сланци, Велико
Село, Вишњица). У периоду од 9. јуна до 5. августа болест је потврђена на 157
газдинстава, односно код 223 домаће и код 110 дивљих свиња, те је посумњано
да су активности људи уз неадекватне биосигурносне мере на газдинствима
допринеле настанку епизоотије. Током јула и августа 2023. године зараза је
потврђена и код дивљих и домаћих свиња на територијама општина Земун,
Гроцка, Чукарица и Обреновац, где је регистровано 34 позитивна газдинстава и
једно ловиште. Од 26. септембра до 28. децембра није било нових случајева
заразе на епизоотиолошком подручју Града Београда, те је зараза одјављена 26.
децембра, односно 3 месеца после последњег потврђеног случаја АКС.
AB  - African swine fever (ASF) was first confirmed in the Republic of Serbia in July 2019,
in domestic pigs settled within the Belgrade epizootiology area. Through an
epizootiological investigation, it was concluded that the outbreak was a result of
irresponsible human behaviour which led to the introduction of the virus from
surrounding countries and its further spread. In the first wave, three outbreaks were
detected in three villages and by implementing regulatory measures for disease
control, the infection was successfully resolved in December 2019. Although the virus
re-entered from neighboring countries and spread across the northeast, central,
eastern, and southern regions, there were no new disease outbreaks in the Belgrade
epizootiological unit during the 2020-2022 period. Despite efforts to control the
infection, the disease reappeared in June 2023, spreading rapidly among wild boars
and domestic pigs in various settlements and hunting grounds of Palilula municipality.
During the two-month period, the disease was confirmed on 157 holdings,
respectively in 223 domestic pigs and 110 wild boars, implying the significance of
human behaviour and poor biosecurity measures on the epizootic spread of the
disease. During July and August 2023, the infection was confirmed in wild boars and
domestic pigs in the municipalities Zemun, Grocka, Čukarica, and Obrenovac, where
34 holdings and one hunting ground were declared positive. From September 26th to
December 28th, there were no new cases of infection in the Belgrade epizootiology
area, and the infection was resolved on December 26th, i.e. 3 months after the last
confirmed case of ASF.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
T1  - Afrička kuga svinja - epizootiološka situacija na teritoriji okruga grada Beograda u periodu od 2019. do 2023. godine
T1  - African swine fever - epizootiological situation in the Belgrade city area in the period from 2019 to 2023
EP  - 91
SP  - 90
ER  - 

@conference{
author = "Maksimović Zorić, Jelena and Veljović, Ljubiša and Glišić, Dimitrije and Kureljušić, Branislav and Jezdimirović, Nemanja and Stanojević, Slobodan and Maletić, Jelena and Ninković, Milan and Aksić, Đorđe and Savić, Božidar",
year = "2024",
abstract = "Афричка куга свиња (АКС) је први пут потврђена на територији Републике
Србије у јулу 2019. године, код домаћих свиња на епизоотиолошком подручју
Града Београда. Епизоотиолошким увиђајем је установљено да је неодговорно
понашање људи довело до уноса вируса из околних земаља и ширења заразе. У
првом таласу регистрована су жаришта у три насељена места, а применом
законом прописаних мера, болест је стављена под контролу и одјављена
децембра 2019. године. Упркос поновном уносу вируса из околних земаља и
ширењу заразе у популацијама дивљих и домаћих свиња у североисточним,
централним, источним и јужним деловима земље, ситуација на територији
епизоотиолошког подручја Града Београда у периоду од 2020. до 2022. године
је била стабилна. Поновно избијање заразе десило се 9. јуна 2023. године када
је болест потврђена код угинуле дивље свиње пронађене у ловишту
"Панчевачки рит", на територији општине Палилула. Већ 12. јуна потврђен је и
први случај код екстензивно узгајаних домаћих свиња у насљеном месту
Крњача. Зараза је убрзо потврђена и код дивљих свиња суседног ловишта "Рит"
и домаћих свиња других насељених места општине Палилула (Бесни Фок,
Дунавац, Јабучки Рит, Ковилово, Падинска Скела, Борча, Овча, Сланци, Велико
Село, Вишњица). У периоду од 9. јуна до 5. августа болест је потврђена на 157
газдинстава, односно код 223 домаће и код 110 дивљих свиња, те је посумњано
да су активности људи уз неадекватне биосигурносне мере на газдинствима
допринеле настанку епизоотије. Током јула и августа 2023. године зараза је
потврђена и код дивљих и домаћих свиња на територијама општина Земун,
Гроцка, Чукарица и Обреновац, где је регистровано 34 позитивна газдинстава и
једно ловиште. Од 26. септембра до 28. децембра није било нових случајева
заразе на епизоотиолошком подручју Града Београда, те је зараза одјављена 26.
децембра, односно 3 месеца после последњег потврђеног случаја АКС., African swine fever (ASF) was first confirmed in the Republic of Serbia in July 2019,
in domestic pigs settled within the Belgrade epizootiology area. Through an
epizootiological investigation, it was concluded that the outbreak was a result of
irresponsible human behaviour which led to the introduction of the virus from
surrounding countries and its further spread. In the first wave, three outbreaks were
detected in three villages and by implementing regulatory measures for disease
control, the infection was successfully resolved in December 2019. Although the virus
re-entered from neighboring countries and spread across the northeast, central,
eastern, and southern regions, there were no new disease outbreaks in the Belgrade
epizootiological unit during the 2020-2022 period. Despite efforts to control the
infection, the disease reappeared in June 2023, spreading rapidly among wild boars
and domestic pigs in various settlements and hunting grounds of Palilula municipality.
During the two-month period, the disease was confirmed on 157 holdings,
respectively in 223 domestic pigs and 110 wild boars, implying the significance of
human behaviour and poor biosecurity measures on the epizootic spread of the
disease. During July and August 2023, the infection was confirmed in wild boars and
domestic pigs in the municipalities Zemun, Grocka, Čukarica, and Obrenovac, where
34 holdings and one hunting ground were declared positive. From September 26th to
December 28th, there were no new cases of infection in the Belgrade epizootiology
area, and the infection was resolved on December 26th, i.e. 3 months after the last
confirmed case of ASF.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.",
title = "Afrička kuga svinja - epizootiološka situacija na teritoriji okruga grada Beograda u periodu od 2019. do 2023. godine, African swine fever - epizootiological situation in the Belgrade city area in the period from 2019 to 2023",
pages = "91-90"
}

Maksimović Zorić, J., Veljović, L., Glišić, D., Kureljušić, B., Jezdimirović, N., Stanojević, S., Maletić, J., Ninković, M., Aksić, Đ.,& Savić, B.. (2024). Afrička kuga svinja - epizootiološka situacija na teritoriji okruga grada Beograda u periodu od 2019. do 2023. godine. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 90-91.

Maksimović Zorić J, Veljović L, Glišić D, Kureljušić B, Jezdimirović N, Stanojević S, Maletić J, Ninković M, Aksić Đ, Savić B. Afrička kuga svinja - epizootiološka situacija na teritoriji okruga grada Beograda u periodu od 2019. do 2023. godine. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.. 2024;:90-91..

Maksimović Zorić, Jelena, Veljović, Ljubiša, Glišić, Dimitrije, Kureljušić, Branislav, Jezdimirović, Nemanja, Stanojević, Slobodan, Maletić, Jelena, Ninković, Milan, Aksić, Đorđe, Savić, Božidar, "Afrička kuga svinja - epizootiološka situacija na teritoriji okruga grada Beograda u periodu od 2019. do 2023. godine" in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god. (2024):90-91.

TY  - JOUR
AU  - Maksimović Zorić, Jelena
AU  - Veljović, Ljubiša
AU  - Radosavljević, Vladimir
AU  - Glišić, Dimitrije
AU  - Kureljušić, Jasna
AU  - Maletić, Jelena
AU  - Savić, Božidar
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/535
AB  - Swine influenza A viruses (swIAVs) are characterised by high mutation rates and zoonotic and pandemicpotential. In order to draw conclusions about virulence in swine and pathogenicity to humans, we examined the existenceof molecular markers and accessory proteins, cross-reactivity with vaccine strains, and resistance to antiviral drugs in five strainsof H1N1 swIAVs. Material and Methods: Amino acid (AA) sequences of five previously genetically characterised swIAVs wereanalysed in MEGA 7.0 software and the Influenza Research Database. Results: Amino acid analysis revealed three virus strainswith 590S/591R polymorphism and T271A substitution within basic polymerase 2 (PB2) AA chains, which cause enhanced virusreplication in mammalian cells. The other two strains possessed D701N and R251K substitutions within PB2 and synthesised PB1-F2protein, which are the factors of increased polymerase activity and virulence in swine. All strains synthesised PB1-N40, PA-N155,PA-N182, and PA-X proteins responsible for enhanced replication in mammalian cells and downregulation of the immune responseof the host. Mutations detected within haemagglutinin antigenic sites imply the antigenic drift of the five analysed viruses in relationto the vaccine strains. All viruses show susceptibility to neuraminidase inhibitors and baloxavir marboxil, which is important insituations of incidental human infections. Conclusion: The detection of virulence markers and accessory proteins in the analysedviruses suggests their higher propensity for replication in mammalian cells, increased virulence, and potential for transmission tohumans, and implies compromised efficacy of influenza vaccines.
PB  - Warsaw : Walter de Gruyter
T2  - Journal of Veterinary Research
T1  - Protein sequence featuresof H1N1 swine influenza A virusesdetected on commercial swine farms in Serbia
EP  - 154
IS  - 2
SP  - 147
VL  - 67
DO  - 10.2478/jvetres-2023-0034
ER  - 

@article{
author = "Maksimović Zorić, Jelena and Veljović, Ljubiša and Radosavljević, Vladimir and Glišić, Dimitrije and Kureljušić, Jasna and Maletić, Jelena and Savić, Božidar",
year = "2023",
abstract = "Swine influenza A viruses (swIAVs) are characterised by high mutation rates and zoonotic and pandemicpotential. In order to draw conclusions about virulence in swine and pathogenicity to humans, we examined the existenceof molecular markers and accessory proteins, cross-reactivity with vaccine strains, and resistance to antiviral drugs in five strainsof H1N1 swIAVs. Material and Methods: Amino acid (AA) sequences of five previously genetically characterised swIAVs wereanalysed in MEGA 7.0 software and the Influenza Research Database. Results: Amino acid analysis revealed three virus strainswith 590S/591R polymorphism and T271A substitution within basic polymerase 2 (PB2) AA chains, which cause enhanced virusreplication in mammalian cells. The other two strains possessed D701N and R251K substitutions within PB2 and synthesised PB1-F2protein, which are the factors of increased polymerase activity and virulence in swine. All strains synthesised PB1-N40, PA-N155,PA-N182, and PA-X proteins responsible for enhanced replication in mammalian cells and downregulation of the immune responseof the host. Mutations detected within haemagglutinin antigenic sites imply the antigenic drift of the five analysed viruses in relationto the vaccine strains. All viruses show susceptibility to neuraminidase inhibitors and baloxavir marboxil, which is important insituations of incidental human infections. Conclusion: The detection of virulence markers and accessory proteins in the analysedviruses suggests their higher propensity for replication in mammalian cells, increased virulence, and potential for transmission tohumans, and implies compromised efficacy of influenza vaccines.",
publisher = "Warsaw : Walter de Gruyter",
journal = "Journal of Veterinary Research",
title = "Protein sequence featuresof H1N1 swine influenza A virusesdetected on commercial swine farms in Serbia",
pages = "154-147",
number = "2",
volume = "67",
doi = "10.2478/jvetres-2023-0034"
}

Maksimović Zorić, J., Veljović, L., Radosavljević, V., Glišić, D., Kureljušić, J., Maletić, J.,& Savić, B.. (2023). Protein sequence featuresof H1N1 swine influenza A virusesdetected on commercial swine farms in Serbia. in Journal of Veterinary Research
Warsaw : Walter de Gruyter., 67(2), 147-154.
https://doi.org/10.2478/jvetres-2023-0034

Maksimović Zorić J, Veljović L, Radosavljević V, Glišić D, Kureljušić J, Maletić J, Savić B. Protein sequence featuresof H1N1 swine influenza A virusesdetected on commercial swine farms in Serbia. in Journal of Veterinary Research. 2023;67(2):147-154.
doi:10.2478/jvetres-2023-0034 .

Maksimović Zorić, Jelena, Veljović, Ljubiša, Radosavljević, Vladimir, Glišić, Dimitrije, Kureljušić, Jasna, Maletić, Jelena, Savić, Božidar, "Protein sequence featuresof H1N1 swine influenza A virusesdetected on commercial swine farms in Serbia" in Journal of Veterinary Research, 67, no. 2 (2023):147-154,
https://doi.org/10.2478/jvetres-2023-0034 . .

TY  - JOUR
AU  - Maksimović Zorić, Jelena
AU  - Milićević, Vesna
AU  - Veljović, Ljubiša
AU  - Radosavljević, Vladimir
AU  - Kureljušić, Branislav
AU  - Stevančević, Ognjen
AU  - Chiapponi, Chiara
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/913
AB  - Swine  influenza  presents  a  very  important  health  and  economic  issue  in  pig  productions  worldwide.  Viruses  that  cause  the  disease  are  genetically  very  diverse  but  usually  belong  to  the  H1N1,  H1N2  and  H3N2 subtype of influenza A viruses. In this study, we sequenced and analyzed the full genome of viruses detected  in  swine  from  seven  commercial  farms.  Through  the  analysis  of  the  complete  sequences  of  internal gene cassette together with previously characterized HA and NA genes we found three different genotypes  amongst  five  completely  sequenced  viruses.  Two  viruses  possessed  a  completely  H1avN1  genotype (40%) and belonged to the H1avN1 lineage, which is prevalent in European swine populations. The other three viruses have arisen through the reassortment of the genes of H1avN1 and H1N1pdm09 lineages. In one sample we detected coinfection with viruses of H3N2 subtype with genes of H1avN1, H1N1pdm09 and A/swine/Gent/1/1984-like H3N2 lineages that presents a potential environment for the generation of a triple reassortant virus. The presence of the H1N1pdm09 origin M gene in this sample implies the potential risk of the introduction of these viruses into the human population. Phylogenetic analysis of internal gene cassette revealed slower evolution within genes of H1N1pdm09 lineage than those of H1avN1 lineage.
PB  - Teramo : Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘G. Caporale’
T2  - Veterinaria Italiana
T1  - Genetic analysis of influenza A viruses in pigs from commercial farms in Serbia
IS  - 2
VL  - 59
DO  - 10.12834/VetIt.2712.17810.2
ER  - 

@article{
author = "Maksimović Zorić, Jelena and Milićević, Vesna and Veljović, Ljubiša and Radosavljević, Vladimir and Kureljušić, Branislav and Stevančević, Ognjen and Chiapponi, Chiara",
year = "2023",
abstract = "Swine  influenza  presents  a  very  important  health  and  economic  issue  in  pig  productions  worldwide.  Viruses  that  cause  the  disease  are  genetically  very  diverse  but  usually  belong  to  the  H1N1,  H1N2  and  H3N2 subtype of influenza A viruses. In this study, we sequenced and analyzed the full genome of viruses detected  in  swine  from  seven  commercial  farms.  Through  the  analysis  of  the  complete  sequences  of  internal gene cassette together with previously characterized HA and NA genes we found three different genotypes  amongst  five  completely  sequenced  viruses.  Two  viruses  possessed  a  completely  H1avN1  genotype (40%) and belonged to the H1avN1 lineage, which is prevalent in European swine populations. The other three viruses have arisen through the reassortment of the genes of H1avN1 and H1N1pdm09 lineages. In one sample we detected coinfection with viruses of H3N2 subtype with genes of H1avN1, H1N1pdm09 and A/swine/Gent/1/1984-like H3N2 lineages that presents a potential environment for the generation of a triple reassortant virus. The presence of the H1N1pdm09 origin M gene in this sample implies the potential risk of the introduction of these viruses into the human population. Phylogenetic analysis of internal gene cassette revealed slower evolution within genes of H1N1pdm09 lineage than those of H1avN1 lineage.",
publisher = "Teramo : Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘G. Caporale’",
journal = "Veterinaria Italiana",
title = "Genetic analysis of influenza A viruses in pigs from commercial farms in Serbia",
number = "2",
volume = "59",
doi = "10.12834/VetIt.2712.17810.2"
}

Maksimović Zorić, J., Milićević, V., Veljović, L., Radosavljević, V., Kureljušić, B., Stevančević, O.,& Chiapponi, C.. (2023). Genetic analysis of influenza A viruses in pigs from commercial farms in Serbia. in Veterinaria Italiana
Teramo : Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘G. Caporale’., 59(2).
https://doi.org/10.12834/VetIt.2712.17810.2

Maksimović Zorić J, Milićević V, Veljović L, Radosavljević V, Kureljušić B, Stevančević O, Chiapponi C. Genetic analysis of influenza A viruses in pigs from commercial farms in Serbia. in Veterinaria Italiana. 2023;59(2).
doi:10.12834/VetIt.2712.17810.2 .

Maksimović Zorić, Jelena, Milićević, Vesna, Veljović, Ljubiša, Radosavljević, Vladimir, Kureljušić, Branislav, Stevančević, Ognjen, Chiapponi, Chiara, "Genetic analysis of influenza A viruses in pigs from commercial farms in Serbia" in Veterinaria Italiana, 59, no. 2 (2023),
https://doi.org/10.12834/VetIt.2712.17810.2 . .

TY  - JOUR
AU  - Veljović, Ljubiša
AU  - Maksimović Zorić, Jelena
AU  - Glišić, Dimitrije
AU  - Nišavić, Jakov
AU  - Maletić, Jelena
AU  - Milićević, Vesna
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/906
AB  - Schmallenberg disease is an infectious disease of cattle, sheep, and goats of viral
aetiology caused by the Schmallenberg virus that belongs to the family Bunyaviridae and
the genus Ortobunyavirus. Schmallenberg disease is a vector-borne disease transmitted by
midges from the genus Culicoides but also by other hematophagous insects. The disease
has a seasonal character and most often occurs in the warm months, from late spring
to autumn. In this study, we investigated the seroprevalence of Schmallenberg disease
in sheep in the epizootic area of Belgrade in the period from 2017 to 2022. A total of
600 sheep serums from the serum bank were tested, 100 serum samples from each year.
Sera were tested by commercial ELISA test for the detection of specific antibodies
against the Schmallenberg virus. The results showed an average annual seroprevalence
of 24.5% in sheep in the Belgrade area in these six years with an increasing trend
predicted for the next years.
PB  - Belgrade : Faculty of Veterinary Medicine, University of Belgrade
T2  - Acta Veterinaria-Beograd
T1  - Seroprevalence of Schmallenberg virus in sheep in Belgrade epizootic area
EP  - 510
IS  - 4
SP  - 502
VL  - 73
DO  - 10.2478/acve-2023-0038
ER  - 

@article{
author = "Veljović, Ljubiša and Maksimović Zorić, Jelena and Glišić, Dimitrije and Nišavić, Jakov and Maletić, Jelena and Milićević, Vesna",
year = "2023",
abstract = "Schmallenberg disease is an infectious disease of cattle, sheep, and goats of viral
aetiology caused by the Schmallenberg virus that belongs to the family Bunyaviridae and
the genus Ortobunyavirus. Schmallenberg disease is a vector-borne disease transmitted by
midges from the genus Culicoides but also by other hematophagous insects. The disease
has a seasonal character and most often occurs in the warm months, from late spring
to autumn. In this study, we investigated the seroprevalence of Schmallenberg disease
in sheep in the epizootic area of Belgrade in the period from 2017 to 2022. A total of
600 sheep serums from the serum bank were tested, 100 serum samples from each year.
Sera were tested by commercial ELISA test for the detection of specific antibodies
against the Schmallenberg virus. The results showed an average annual seroprevalence
of 24.5% in sheep in the Belgrade area in these six years with an increasing trend
predicted for the next years.",
publisher = "Belgrade : Faculty of Veterinary Medicine, University of Belgrade",
journal = "Acta Veterinaria-Beograd",
title = "Seroprevalence of Schmallenberg virus in sheep in Belgrade epizootic area",
pages = "510-502",
number = "4",
volume = "73",
doi = "10.2478/acve-2023-0038"
}

Veljović, L., Maksimović Zorić, J., Glišić, D., Nišavić, J., Maletić, J.,& Milićević, V.. (2023). Seroprevalence of Schmallenberg virus in sheep in Belgrade epizootic area. in Acta Veterinaria-Beograd
Belgrade : Faculty of Veterinary Medicine, University of Belgrade., 73(4), 502-510.
https://doi.org/10.2478/acve-2023-0038

Veljović L, Maksimović Zorić J, Glišić D, Nišavić J, Maletić J, Milićević V. Seroprevalence of Schmallenberg virus in sheep in Belgrade epizootic area. in Acta Veterinaria-Beograd. 2023;73(4):502-510.
doi:10.2478/acve-2023-0038 .

Veljović, Ljubiša, Maksimović Zorić, Jelena, Glišić, Dimitrije, Nišavić, Jakov, Maletić, Jelena, Milićević, Vesna, "Seroprevalence of Schmallenberg virus in sheep in Belgrade epizootic area" in Acta Veterinaria-Beograd, 73, no. 4 (2023):502-510,
https://doi.org/10.2478/acve-2023-0038 . .

TY  - JOUR
AU  - Glišić, Dimitrije
AU  - Veljović, Ljubiša
AU  - Milovanović, Bojan
AU  - Ninković, Milan
AU  - Maletić, Jelena
AU  - Kureljušić, Branislav
AU  - Milićević, Vesna
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/849
AB  - Monitoring the health of swine herds is essential to ensure good manufacturing
practices. Traditionally, active and passive surveillance on farms involved invasive
sampling methods, where specific animals were selected, restrained, and sampled.
However, with the increasing intensity of swine production, alternative methods for
effective herd surveillance became necessary.
Non-invasive sampling provides a convenient and cost-effective approach to monitor
the entire herd without compromising animal welfare, while still obtaining suitable
samples for testing. Oral fluids have been widely used in both human and livestock
health surveillance for various viral pathogens, including significant diseases. Nasal
wipes (NW) utilize different cloth materials soaked in phosphate-buffered saline
(PBS) or tissue culture medium with antibiotics and antimycotics to sample for swine
influenza virus (SIV). Udder skin wipes (USW) offer an alternative method to assess
the health status of piglets in a litter. During routine procedures such as tail docking
and castration, a mixture of blood and serum can be collected, known as process fluids
(PF), which has proven successful in monitoring herds for the presence of porcine
reproductive and respiratory syndrome virus (PRRSV). Furthermore, air sampling has emerged as a novel technique to detect pathogens in various farming systems and
animal species. This method offers the advantage of obtaining diagnostic samples
without direct animal contact.
By employing these non-invasive sampling methods, swine producers can implement
effective surveillance strategies while maintaining animal welfare standards and
obtaining reliable diagnostic information.
PB  - Beograd : Fakultet veterinarske medicine, Univerzitet u Beogradu
PB  - Beograd : Veterinarska komora Srbije
T2  - Veterinarski glasnik
T1  - Overview of non-invasive sampling methods used in intensive swine farming
EP  - 108
IS  - 2
SP  - 97
VL  - 77
DO  - 10.2298/VETGL230614004G
ER  - 

@article{
author = "Glišić, Dimitrije and Veljović, Ljubiša and Milovanović, Bojan and Ninković, Milan and Maletić, Jelena and Kureljušić, Branislav and Milićević, Vesna",
year = "2023",
abstract = "Monitoring the health of swine herds is essential to ensure good manufacturing
practices. Traditionally, active and passive surveillance on farms involved invasive
sampling methods, where specific animals were selected, restrained, and sampled.
However, with the increasing intensity of swine production, alternative methods for
effective herd surveillance became necessary.
Non-invasive sampling provides a convenient and cost-effective approach to monitor
the entire herd without compromising animal welfare, while still obtaining suitable
samples for testing. Oral fluids have been widely used in both human and livestock
health surveillance for various viral pathogens, including significant diseases. Nasal
wipes (NW) utilize different cloth materials soaked in phosphate-buffered saline
(PBS) or tissue culture medium with antibiotics and antimycotics to sample for swine
influenza virus (SIV). Udder skin wipes (USW) offer an alternative method to assess
the health status of piglets in a litter. During routine procedures such as tail docking
and castration, a mixture of blood and serum can be collected, known as process fluids
(PF), which has proven successful in monitoring herds for the presence of porcine
reproductive and respiratory syndrome virus (PRRSV). Furthermore, air sampling has emerged as a novel technique to detect pathogens in various farming systems and
animal species. This method offers the advantage of obtaining diagnostic samples
without direct animal contact.
By employing these non-invasive sampling methods, swine producers can implement
effective surveillance strategies while maintaining animal welfare standards and
obtaining reliable diagnostic information.",
publisher = "Beograd : Fakultet veterinarske medicine, Univerzitet u Beogradu, Beograd : Veterinarska komora Srbije",
journal = "Veterinarski glasnik",
title = "Overview of non-invasive sampling methods used in intensive swine farming",
pages = "108-97",
number = "2",
volume = "77",
doi = "10.2298/VETGL230614004G"
}

Glišić, D., Veljović, L., Milovanović, B., Ninković, M., Maletić, J., Kureljušić, B.,& Milićević, V.. (2023). Overview of non-invasive sampling methods used in intensive swine farming. in Veterinarski glasnik
Beograd : Fakultet veterinarske medicine, Univerzitet u Beogradu., 77(2), 97-108.
https://doi.org/10.2298/VETGL230614004G

Glišić D, Veljović L, Milovanović B, Ninković M, Maletić J, Kureljušić B, Milićević V. Overview of non-invasive sampling methods used in intensive swine farming. in Veterinarski glasnik. 2023;77(2):97-108.
doi:10.2298/VETGL230614004G .

Glišić, Dimitrije, Veljović, Ljubiša, Milovanović, Bojan, Ninković, Milan, Maletić, Jelena, Kureljušić, Branislav, Milićević, Vesna, "Overview of non-invasive sampling methods used in intensive swine farming" in Veterinarski glasnik, 77, no. 2 (2023):97-108,
https://doi.org/10.2298/VETGL230614004G . .

TY  - JOUR
AU  - Milićević, Vesna
AU  - Glišić, Dimitrije
AU  - Veljović, Ljubiša
AU  - Maletić, Jelena
AU  - Kureljušić, Branislav
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/835
AB  - Rabies is a viral disease of the central nervous system of all warm-blooded animals
and one of the oldest and most important zoonoses. In the Republic of Serbia, rabies is controlled by compulsory vaccination of dogs and cats and oral vaccination of
wild carnivores, which has been implemented since 2010. In the period 2009–2018,
367 rabies cases were recorded. The last rabies case in Serbia was recorded in 2018
in a red fox (Vulpes vulpes), while the last case in dogs was diagnosed in 2011. This
study aimed to perform a retrospective phylogenetic analysis of G and N genes of
the rabies virus from Serbia from 2009 to 2018 to understand sources and pathways
of infection better and to enable molecular virus tracing in the future based on the
association of rabies virus genetic lineages with the geographic distribution. For the
phylogenetic analysis of the rabies virus, 14 historically positive brain samples of red
foxes from 2009 to 2018, collected in central Serbia, were used. All field strains from
Serbia were identified as classic rabies virus and grouped within the Cosmopolitan lineage. Phylogenetic analysis of N gene sequences revealed that 13 rabies virus strains
(92.9%) from Serbia belonged to the EE sub-lineage of isolates, while one virus (7.1%)
ON988027 from 2011 clustered together with isolates from the WE sub-lineage.
PB  - Wiley
T2  - Zoonoses and Public Health
T1  - Retrospective phylogenetic analysis of rabies virus G and N genes from Serbia
EP  - 704
IS  - 8
SP  - 699
VL  - 70
DO  - 10.1111/zph.13061
ER  - 

@article{
author = "Milićević, Vesna and Glišić, Dimitrije and Veljović, Ljubiša and Maletić, Jelena and Kureljušić, Branislav",
year = "2023",
abstract = "Rabies is a viral disease of the central nervous system of all warm-blooded animals
and one of the oldest and most important zoonoses. In the Republic of Serbia, rabies is controlled by compulsory vaccination of dogs and cats and oral vaccination of
wild carnivores, which has been implemented since 2010. In the period 2009–2018,
367 rabies cases were recorded. The last rabies case in Serbia was recorded in 2018
in a red fox (Vulpes vulpes), while the last case in dogs was diagnosed in 2011. This
study aimed to perform a retrospective phylogenetic analysis of G and N genes of
the rabies virus from Serbia from 2009 to 2018 to understand sources and pathways
of infection better and to enable molecular virus tracing in the future based on the
association of rabies virus genetic lineages with the geographic distribution. For the
phylogenetic analysis of the rabies virus, 14 historically positive brain samples of red
foxes from 2009 to 2018, collected in central Serbia, were used. All field strains from
Serbia were identified as classic rabies virus and grouped within the Cosmopolitan lineage. Phylogenetic analysis of N gene sequences revealed that 13 rabies virus strains
(92.9%) from Serbia belonged to the EE sub-lineage of isolates, while one virus (7.1%)
ON988027 from 2011 clustered together with isolates from the WE sub-lineage.",
publisher = "Wiley",
journal = "Zoonoses and Public Health",
title = "Retrospective phylogenetic analysis of rabies virus G and N genes from Serbia",
pages = "704-699",
number = "8",
volume = "70",
doi = "10.1111/zph.13061"
}

Milićević, V., Glišić, D., Veljović, L., Maletić, J.,& Kureljušić, B.. (2023). Retrospective phylogenetic analysis of rabies virus G and N genes from Serbia. in Zoonoses and Public Health
Wiley., 70(8), 699-704.
https://doi.org/10.1111/zph.13061

Milićević V, Glišić D, Veljović L, Maletić J, Kureljušić B. Retrospective phylogenetic analysis of rabies virus G and N genes from Serbia. in Zoonoses and Public Health. 2023;70(8):699-704.
doi:10.1111/zph.13061 .

Milićević, Vesna, Glišić, Dimitrije, Veljović, Ljubiša, Maletić, Jelena, Kureljušić, Branislav, "Retrospective phylogenetic analysis of rabies virus G and N genes from Serbia" in Zoonoses and Public Health, 70, no. 8 (2023):699-704,
https://doi.org/10.1111/zph.13061 . .

TY  - CONF
AU  - Milićević, Vesna
AU  - Zurovac Sapundžić, Zorana
AU  - Glišić, Dimitrije
AU  - Veljović, Ljubiša
AU  - Ninković, Milan
AU  - Žutić, Jadranka
AU  - Mirčeta, Jovan
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/824
AB  - While domestic and wild animals share the same pathogens, wildlife is recognized as the reservoir of many
diseases. Considering their role as indicators, victims, bridge hosts, or maintenance hosts, the monitoring of
wildlife should be an integrated part of country surveillance and disease control strategy. Thus, the main aim
of this study was to determine the presence and the spread of selected infectious diseases in red deer
population in Serbia through seroprevalence estimation.
A random geographical collection of samples from 123 shot red deer was organized during the hunting season from
August 2022 to February 2023. The blood was collected directly from the heart using a syringe and needle. For each
sample, data on geolocation, species, age, and sex were collected. Sera samples were decanted after centrifugation at
2000 g for 10 minutes and tested for the presence of specific antibodies against Capripox virus (ELISA: ID Screen®
Capripox Double Antigen Multi-species, IDvet), West Nile fever virus (ELISA: INgezim West Nile Compac, Ingenasa),
Schmallenberg virus (ELISA: ID Screen® Schmallenberg virus Indirect Multi-species, IDvet), Bluetongue disease virus
(ELISA: INgezim BTV DR, Ingenasa), Bovine viral diarrhea virus (ELISA: PrioCHECK™ Ruminant BVD p80 Ab Serum & Milk,
Prionics), Bovine Herpesvirus-1 (ELISA: ID Screen® IBR gB Competition, IDvet), Q fever (ELISA: ID Screen® Q Fever
Indirect Multi-species, IDvet), Brucella spp. (RBT; ELISA: INgezim® Brucella Compac 2.0 Ingenasa) and Leptospira spp.
(MAT).
Specific antibodies against Capripox virus, Bovine Viral Diarrhea Virus and Leptospira spp. were not detected
in any of tested samples. Seroprevalence of Brucella spp. infection was 0.8%. Q fever infection was indirectly
confirmed in 3 red deer (2.4%) from the same hunting ground. Seroprevalence of vector-borne diseases in
the red deer population was 18.5% for Schmallenberg disease, 39% for Bluetongue disease, and 58.5% for
West Nile fever. Bovine Herpesvirus-1 antibodies were detected in 43.1% of samples.
The red deer population can be considered the reservoir of vector-borne viral diseases such as Bluetongue
disease, West Nile fever and Schmallenberg disease. Based on estimated seroprevalence, red deer should
not be considered a risk for maintaining Q fever, Brucellosis, Capripox virus, Bovine Viral Diarrhea Virus and
Leptospira spp. infection. The results of the present study indicate that the red deer population should be
thoughtfully considered during livestock disease eradication programs, particularly infectious bovine
rhinotracheitis and Bluetongue disease.
PB  - Novi Sad : The Scienfic Veterinary Institute Novi Sad
C3  - EPIZONE Annual Meeting "New Perspectives for the New Era"
T1  - Seroprevalence of selected infectious diseases in red deer population in Serbia
EP  - 78
SP  - 78
ER  - 

@conference{
author = "Milićević, Vesna and Zurovac Sapundžić, Zorana and Glišić, Dimitrije and Veljović, Ljubiša and Ninković, Milan and Žutić, Jadranka and Mirčeta, Jovan",
year = "2023",
abstract = "While domestic and wild animals share the same pathogens, wildlife is recognized as the reservoir of many
diseases. Considering their role as indicators, victims, bridge hosts, or maintenance hosts, the monitoring of
wildlife should be an integrated part of country surveillance and disease control strategy. Thus, the main aim
of this study was to determine the presence and the spread of selected infectious diseases in red deer
population in Serbia through seroprevalence estimation.
A random geographical collection of samples from 123 shot red deer was organized during the hunting season from
August 2022 to February 2023. The blood was collected directly from the heart using a syringe and needle. For each
sample, data on geolocation, species, age, and sex were collected. Sera samples were decanted after centrifugation at
2000 g for 10 minutes and tested for the presence of specific antibodies against Capripox virus (ELISA: ID Screen®
Capripox Double Antigen Multi-species, IDvet), West Nile fever virus (ELISA: INgezim West Nile Compac, Ingenasa),
Schmallenberg virus (ELISA: ID Screen® Schmallenberg virus Indirect Multi-species, IDvet), Bluetongue disease virus
(ELISA: INgezim BTV DR, Ingenasa), Bovine viral diarrhea virus (ELISA: PrioCHECK™ Ruminant BVD p80 Ab Serum & Milk,
Prionics), Bovine Herpesvirus-1 (ELISA: ID Screen® IBR gB Competition, IDvet), Q fever (ELISA: ID Screen® Q Fever
Indirect Multi-species, IDvet), Brucella spp. (RBT; ELISA: INgezim® Brucella Compac 2.0 Ingenasa) and Leptospira spp.
(MAT).
Specific antibodies against Capripox virus, Bovine Viral Diarrhea Virus and Leptospira spp. were not detected
in any of tested samples. Seroprevalence of Brucella spp. infection was 0.8%. Q fever infection was indirectly
confirmed in 3 red deer (2.4%) from the same hunting ground. Seroprevalence of vector-borne diseases in
the red deer population was 18.5% for Schmallenberg disease, 39% for Bluetongue disease, and 58.5% for
West Nile fever. Bovine Herpesvirus-1 antibodies were detected in 43.1% of samples.
The red deer population can be considered the reservoir of vector-borne viral diseases such as Bluetongue
disease, West Nile fever and Schmallenberg disease. Based on estimated seroprevalence, red deer should
not be considered a risk for maintaining Q fever, Brucellosis, Capripox virus, Bovine Viral Diarrhea Virus and
Leptospira spp. infection. The results of the present study indicate that the red deer population should be
thoughtfully considered during livestock disease eradication programs, particularly infectious bovine
rhinotracheitis and Bluetongue disease.",
publisher = "Novi Sad : The Scienfic Veterinary Institute Novi Sad",
journal = "EPIZONE Annual Meeting "New Perspectives for the New Era"",
title = "Seroprevalence of selected infectious diseases in red deer population in Serbia",
pages = "78-78"
}

Milićević, V., Zurovac Sapundžić, Z., Glišić, D., Veljović, L., Ninković, M., Žutić, J.,& Mirčeta, J.. (2023). Seroprevalence of selected infectious diseases in red deer population in Serbia. in EPIZONE Annual Meeting "New Perspectives for the New Era"
Novi Sad : The Scienfic Veterinary Institute Novi Sad., 78-78.

Milićević V, Zurovac Sapundžić Z, Glišić D, Veljović L, Ninković M, Žutić J, Mirčeta J. Seroprevalence of selected infectious diseases in red deer population in Serbia. in EPIZONE Annual Meeting "New Perspectives for the New Era". 2023;:78-78..

Milićević, Vesna, Zurovac Sapundžić, Zorana, Glišić, Dimitrije, Veljović, Ljubiša, Ninković, Milan, Žutić, Jadranka, Mirčeta, Jovan, "Seroprevalence of selected infectious diseases in red deer population in Serbia" in EPIZONE Annual Meeting "New Perspectives for the New Era" (2023):78-78.

TY  - CONF
AU  - Milićević, Vesna
AU  - Glišić, Dimitrije
AU  - Milovanović, Bojan
AU  - Veljović, Ljubiša
AU  - Maletić, Jelena
AU  - Kureljušić, Branislav
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/803
AB  - Non-invasive sampling is increasingly used in domestic and wild animals. Traditional sampling methods are labour, time-consuming, and require trained personnel. Oral fluid is an alternative sample that can replace nasal swabs. Air sampling with special device and testing represents the newest method of non-invasive sampling, which is particularly useful and validated when testing pigs. Udder skin sampling is a new sampling approach that provides insight into litters of pigs before weaning, containing their nasal and oral secretions.
PB  - ECPHM
C3  - Proceedings : ESPHM : 14th European symposium of porcine health management
T1  - Porcine viral diseases can be successfully  monitored using non-invasive sampling strategy
EP  - 317
SP  - 317
ER  - 

@conference{
author = "Milićević, Vesna and Glišić, Dimitrije and Milovanović, Bojan and Veljović, Ljubiša and Maletić, Jelena and Kureljušić, Branislav",
year = "2023",
abstract = "Non-invasive sampling is increasingly used in domestic and wild animals. Traditional sampling methods are labour, time-consuming, and require trained personnel. Oral fluid is an alternative sample that can replace nasal swabs. Air sampling with special device and testing represents the newest method of non-invasive sampling, which is particularly useful and validated when testing pigs. Udder skin sampling is a new sampling approach that provides insight into litters of pigs before weaning, containing their nasal and oral secretions.",
publisher = "ECPHM",
journal = "Proceedings : ESPHM : 14th European symposium of porcine health management",
title = "Porcine viral diseases can be successfully  monitored using non-invasive sampling strategy",
pages = "317-317"
}

Milićević, V., Glišić, D., Milovanović, B., Veljović, L., Maletić, J.,& Kureljušić, B.. (2023). Porcine viral diseases can be successfully  monitored using non-invasive sampling strategy. in Proceedings : ESPHM : 14th European symposium of porcine health management
ECPHM., 317-317.

Milićević V, Glišić D, Milovanović B, Veljović L, Maletić J, Kureljušić B. Porcine viral diseases can be successfully  monitored using non-invasive sampling strategy. in Proceedings : ESPHM : 14th European symposium of porcine health management. 2023;:317-317..

Milićević, Vesna, Glišić, Dimitrije, Milovanović, Bojan, Veljović, Ljubiša, Maletić, Jelena, Kureljušić, Branislav, "Porcine viral diseases can be successfully  monitored using non-invasive sampling strategy" in Proceedings : ESPHM : 14th European symposium of porcine health management (2023):317-317.

TY  - JOUR
AU  - Milićević, Vesna
AU  - Glišić, Dimitrije
AU  - Veljović, Ljubiša
AU  - Vasić, Ana
AU  - Milovanović, Bojan
AU  - Kureljušić, Branislav
AU  - Paunović, Milan
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/777
AB  - Parvoviruses are among the major animal pathogens that can cause considerable health disorders ranging from subclinical
to lethal in domestic and wild animals. Golden jackal (Canis aureus), an expanding European species, is a reservoir of many
pathogens, including vector-borne diseases and zoonoses. Given the importance of parvovirus infections in dogs and cats,
this study aimed to unfold the virus prevalence and molecular characterisation in the golden jackal population in Serbia.
The spleen samples from 68 hunted jackals during 2022/2023 were tested for the VP2-specific genome region of Protoparvovirus
carnivoran 1 by PCR. BLAST analysis of partial VP2 sequences obtained from three animals (4.4%) revealed the
highest similarity to Protoparvovirus carnivoran 1, genogroup Feline panleukopenia virus, which is the second report on
FPV infection in jackals. Based on specific amino acid residues within partial VP2, the jackals’ Protoparvovirus carnivoran
1 was also classified as FPV. One jackal’s strain showed two synonymous mutations at positions 699 and 1167. Although
species cross-transmission could not be established, jackals’ health should be maintained by preventing the transmission
of viruses to native species and vice versa. Although jackals are considered pests, their role as natural cleaners is of greater
importance. Therefore, further monitoring of their health is needed to understand the influence of infectious diseases on
population dynamics and to determine the relationship between domestic predators and jackals and the direction of crossspecies
transmission.
PB  - Springer
T2  - Veterinary Research Communications
T1  - Protoparvovirus carnivoran 1 infection of golden jackals Canis aureus in Serbia
DO  - 10.1007/s11259-023-10249-0
ER  - 

@article{
author = "Milićević, Vesna and Glišić, Dimitrije and Veljović, Ljubiša and Vasić, Ana and Milovanović, Bojan and Kureljušić, Branislav and Paunović, Milan",
year = "2023",
abstract = "Parvoviruses are among the major animal pathogens that can cause considerable health disorders ranging from subclinical
to lethal in domestic and wild animals. Golden jackal (Canis aureus), an expanding European species, is a reservoir of many
pathogens, including vector-borne diseases and zoonoses. Given the importance of parvovirus infections in dogs and cats,
this study aimed to unfold the virus prevalence and molecular characterisation in the golden jackal population in Serbia.
The spleen samples from 68 hunted jackals during 2022/2023 were tested for the VP2-specific genome region of Protoparvovirus
carnivoran 1 by PCR. BLAST analysis of partial VP2 sequences obtained from three animals (4.4%) revealed the
highest similarity to Protoparvovirus carnivoran 1, genogroup Feline panleukopenia virus, which is the second report on
FPV infection in jackals. Based on specific amino acid residues within partial VP2, the jackals’ Protoparvovirus carnivoran
1 was also classified as FPV. One jackal’s strain showed two synonymous mutations at positions 699 and 1167. Although
species cross-transmission could not be established, jackals’ health should be maintained by preventing the transmission
of viruses to native species and vice versa. Although jackals are considered pests, their role as natural cleaners is of greater
importance. Therefore, further monitoring of their health is needed to understand the influence of infectious diseases on
population dynamics and to determine the relationship between domestic predators and jackals and the direction of crossspecies
transmission.",
publisher = "Springer",
journal = "Veterinary Research Communications",
title = "Protoparvovirus carnivoran 1 infection of golden jackals Canis aureus in Serbia",
doi = "10.1007/s11259-023-10249-0"
}

Milićević, V., Glišić, D., Veljović, L., Vasić, A., Milovanović, B., Kureljušić, B.,& Paunović, M.. (2023). Protoparvovirus carnivoran 1 infection of golden jackals Canis aureus in Serbia. in Veterinary Research Communications
Springer..
https://doi.org/10.1007/s11259-023-10249-0

Milićević V, Glišić D, Veljović L, Vasić A, Milovanović B, Kureljušić B, Paunović M. Protoparvovirus carnivoran 1 infection of golden jackals Canis aureus in Serbia. in Veterinary Research Communications. 2023;.
doi:10.1007/s11259-023-10249-0 .

Milićević, Vesna, Glišić, Dimitrije, Veljović, Ljubiša, Vasić, Ana, Milovanović, Bojan, Kureljušić, Branislav, Paunović, Milan, "Protoparvovirus carnivoran 1 infection of golden jackals Canis aureus in Serbia" in Veterinary Research Communications (2023),
https://doi.org/10.1007/s11259-023-10249-0 . .

TY  - CONF
AU  - Milićević, Vesna
AU  - Kureljušić, Branislav
AU  - Glišić, Dimitrije
AU  - Milovanović, Bojan
AU  - Veljović, Ljubiša
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/768
AB  - Foot-and-mouth disease (FMD) is a highly contagious viral disease of domestic
and wild ungulates and one of the most important diseases of animals in international
trade because it spreads very quickly and causes significant economic losses. In Africa,
Asia, the Middle East and a limited area of South America, FMD is an enzootic disease
and is controlled by vaccination. It is estimated that 77% of the world's livestock
population is affected by FMD (WOAH, 2022). Countries that are free of FMD without
vaccination are constantly at risk and invest heavily in preventing the introduction of
the virus. The causative agent of this disease is the FMD virus, which belongs to the
genus Aphtovirus and the family Picornaviridae. The virus is relatively resistant and
can survive in contaminated materials and the environment for several months under
ideal conditions. There are 7 serotypes and more than 60 subtypes of the FMD virus.
Immunity against one type of virus does not protect the animal from other serotypes
or subtypes.
The last cases of FMD in Europe were recorded in 2001 in the United Kingdom
(UK) and in 2011 in Bulgaria. FMD in the UK was registered on 2000 farms, as a result
of which about seven million animals were killed (Sutmoller et al., 2003). In Bulgaria,
the first case was detected in a wild boar on the border with Turkey, and then the
disease spread to domestic animals, cattle, sheep, goats and pigs on 11 farms. 2230 animals were killed in Bulgaria. Although sporadic cases of the disease were later
detected in wild game, it has not been proven that the FMD virus can be maintained in
wild animal populations (Elbers et al., 2003). The most common mechanism of the
spread of FMD is through direct contact, which can take the form of mechanical
transmission of the virus from infected to healthy animals through the skin and
mucous membranes or by aerosol and even by wind. The most important source of
infection is the contents of the vesicle, but the virus is also excreted in all
secretions/excreta, as well as in exhaled air. Cattle excrete less virus in their breath,
but, unlike pigs, they are very susceptible to infection through the respiratory route.
The minimum infectious dose of airborne virus for ruminants is only 10 TCID50, while
for pigs it is 103 TCID50 (Alekandersen et al., 2003). On the other hand, pigs are
considered an amplifier of the virus because they produce and excrete the most virus,
and therefore represent the greatest risk of spreading the disease. Depending on the
wind speed, infected pigs are a source of infection especially for cattle for miles
around. Such spread was noted in the UK when the disease was transferred from a pig
farm on the Isle of Wight to an area in northern France (Donaldson et al., 1982).
In addition to the pig phenomenon, a persistent form of infection in the
nasopharyngeal epithelium and lymphatic tissue is very important epizootiologically.
Persistent infection is defined as the presence of an infectious virus in the esophagealpharyngeal
(OP) fluid more than 15 or 21 days after infection in vaccinated or infected
cattle. The maximum duration of persistent infection in cattle is 3.5 years, sheep 9
months and goats 4 months (Alekandersen et al., 2003).
Clinically, the disease is characterized by a high body temperature (~
the appearance of vesicles in the oral cavity, feet and teats. After the bursting of the
vesicles, the processes become extremely painful, as a result of which the animals
cannot eat and avoid standing up if they have changes in their hooves. Most of the
affected animals recover, but with significant consequences, especially in milk
production. It is interesting that in young animals, sudden death can be the only sign
due to multifocal myocarditis that occurs before the appearance of vesicles. The
disease in sheep is mostly inapparent, which is why sheep are the species of choice for
serological surveillance of FMD.
In relation to the distribution of viruses, there are 7 enzootic areas (pools)
characterized by virus genotypes that circulate and develop independently within the
area. However, every year, the virus jumps from one area to another, usually through
illegal animal traffic. A very worrying epizootic event was recorded in early 2023 with
the emergence of SAT2/KSIV in Iraq, Jordan and Turkey (ADIS, 2023). The SAT2
epizootic was registered in three districts in Turkey on March 3, 2023, in the east of
the country. Out of 4944 susceptible animals, in all three districts, 7 clinical cases and
no deaths were determined. Analyzes of the nucleotide sequences of viruses from
these areas confirmed that the epizootics were caused by viruses of the SAT2/KSIV
topotype closely related to the viruses from Ethiopia in 2022. Considering that this is
the first occurrence of SAT2 in the mentioned countries where the population is fully
received, there is a justified concern from the rapid spread to other countries in the
region, the buffer zone without FMD in Thrace, as well as further west.
Turkey is the bridge that connects Europe and Asia and as such is very important
epizootiologically. The mass movements of animals in Turkey are always in the
direction of the west, towards the Marmara region and further towards Europe.
Foot and mouth disease is one of the most difficult animal diseases to control.
Different strategies can be applied to control FMD including mass vaccination as is the
case in Turkey.
Traditional measures to prevent the spread are the prohibition of traffic and trade
in animals and animal products from infected areas. Strict control at the border is a
crucial tactical measure because it has been shown that the legal and illegal movement
of animals contributes to the spread of diseases. In case of disease, the "stamping out"
method is applied, with the possibility of ring vaccination.
FMD is always a current topic because of its disastrous consequences in livestock
farming. In the future, due to rapid genetic change among serotypes, the virus will
continue to evolve and cause epizootics in enzootic areas. Therefore, trained
veterinary staff, good infrastructure, trained management and rapid diagnostic testing
in well-equipped laboratories must be ensured before the emergence of SIS. In
addition, the existence of an effective monitoring and surveillance system is crucial.
Serbia is a country free from FMD. Every year, for the purpose of confirming the free
status, surveillance is carried out by serological testing of a representative number of samples, Legislatively, SIŠ is regulated by the Ruleboook on establishing measures for
early detection, diagnosis, prevention of the spread, suppression and eradication of
foot-and-mouth disease ("Official Gazette of the RS", no. 15/2010) and the Rulebook
on establishing a crisis management plan (" Official Gazette of the RS", No. 90/2015)
PB  - Beograd : Srpsko veterinarsko društvo
C3  - 34. Savetovanje veterinara Srbije, Zlatibor, 7-10. septembar 2023
T1  - Slinavka i šap - bolest koja nam stalno preti
T1  - Foot and mouth disease - constantly threatening disease
EP  - 20
SP  - 16
ER  - 

@conference{
author = "Milićević, Vesna and Kureljušić, Branislav and Glišić, Dimitrije and Milovanović, Bojan and Veljović, Ljubiša",
year = "2023",
abstract = "Foot-and-mouth disease (FMD) is a highly contagious viral disease of domestic
and wild ungulates and one of the most important diseases of animals in international
trade because it spreads very quickly and causes significant economic losses. In Africa,
Asia, the Middle East and a limited area of South America, FMD is an enzootic disease
and is controlled by vaccination. It is estimated that 77% of the world's livestock
population is affected by FMD (WOAH, 2022). Countries that are free of FMD without
vaccination are constantly at risk and invest heavily in preventing the introduction of
the virus. The causative agent of this disease is the FMD virus, which belongs to the
genus Aphtovirus and the family Picornaviridae. The virus is relatively resistant and
can survive in contaminated materials and the environment for several months under
ideal conditions. There are 7 serotypes and more than 60 subtypes of the FMD virus.
Immunity against one type of virus does not protect the animal from other serotypes
or subtypes.
The last cases of FMD in Europe were recorded in 2001 in the United Kingdom
(UK) and in 2011 in Bulgaria. FMD in the UK was registered on 2000 farms, as a result
of which about seven million animals were killed (Sutmoller et al., 2003). In Bulgaria,
the first case was detected in a wild boar on the border with Turkey, and then the
disease spread to domestic animals, cattle, sheep, goats and pigs on 11 farms. 2230 animals were killed in Bulgaria. Although sporadic cases of the disease were later
detected in wild game, it has not been proven that the FMD virus can be maintained in
wild animal populations (Elbers et al., 2003). The most common mechanism of the
spread of FMD is through direct contact, which can take the form of mechanical
transmission of the virus from infected to healthy animals through the skin and
mucous membranes or by aerosol and even by wind. The most important source of
infection is the contents of the vesicle, but the virus is also excreted in all
secretions/excreta, as well as in exhaled air. Cattle excrete less virus in their breath,
but, unlike pigs, they are very susceptible to infection through the respiratory route.
The minimum infectious dose of airborne virus for ruminants is only 10 TCID50, while
for pigs it is 103 TCID50 (Alekandersen et al., 2003). On the other hand, pigs are
considered an amplifier of the virus because they produce and excrete the most virus,
and therefore represent the greatest risk of spreading the disease. Depending on the
wind speed, infected pigs are a source of infection especially for cattle for miles
around. Such spread was noted in the UK when the disease was transferred from a pig
farm on the Isle of Wight to an area in northern France (Donaldson et al., 1982).
In addition to the pig phenomenon, a persistent form of infection in the
nasopharyngeal epithelium and lymphatic tissue is very important epizootiologically.
Persistent infection is defined as the presence of an infectious virus in the esophagealpharyngeal
(OP) fluid more than 15 or 21 days after infection in vaccinated or infected
cattle. The maximum duration of persistent infection in cattle is 3.5 years, sheep 9
months and goats 4 months (Alekandersen et al., 2003).
Clinically, the disease is characterized by a high body temperature (~
the appearance of vesicles in the oral cavity, feet and teats. After the bursting of the
vesicles, the processes become extremely painful, as a result of which the animals
cannot eat and avoid standing up if they have changes in their hooves. Most of the
affected animals recover, but with significant consequences, especially in milk
production. It is interesting that in young animals, sudden death can be the only sign
due to multifocal myocarditis that occurs before the appearance of vesicles. The
disease in sheep is mostly inapparent, which is why sheep are the species of choice for
serological surveillance of FMD.
In relation to the distribution of viruses, there are 7 enzootic areas (pools)
characterized by virus genotypes that circulate and develop independently within the
area. However, every year, the virus jumps from one area to another, usually through
illegal animal traffic. A very worrying epizootic event was recorded in early 2023 with
the emergence of SAT2/KSIV in Iraq, Jordan and Turkey (ADIS, 2023). The SAT2
epizootic was registered in three districts in Turkey on March 3, 2023, in the east of
the country. Out of 4944 susceptible animals, in all three districts, 7 clinical cases and
no deaths were determined. Analyzes of the nucleotide sequences of viruses from
these areas confirmed that the epizootics were caused by viruses of the SAT2/KSIV
topotype closely related to the viruses from Ethiopia in 2022. Considering that this is
the first occurrence of SAT2 in the mentioned countries where the population is fully
received, there is a justified concern from the rapid spread to other countries in the
region, the buffer zone without FMD in Thrace, as well as further west.
Turkey is the bridge that connects Europe and Asia and as such is very important
epizootiologically. The mass movements of animals in Turkey are always in the
direction of the west, towards the Marmara region and further towards Europe.
Foot and mouth disease is one of the most difficult animal diseases to control.
Different strategies can be applied to control FMD including mass vaccination as is the
case in Turkey.
Traditional measures to prevent the spread are the prohibition of traffic and trade
in animals and animal products from infected areas. Strict control at the border is a
crucial tactical measure because it has been shown that the legal and illegal movement
of animals contributes to the spread of diseases. In case of disease, the "stamping out"
method is applied, with the possibility of ring vaccination.
FMD is always a current topic because of its disastrous consequences in livestock
farming. In the future, due to rapid genetic change among serotypes, the virus will
continue to evolve and cause epizootics in enzootic areas. Therefore, trained
veterinary staff, good infrastructure, trained management and rapid diagnostic testing
in well-equipped laboratories must be ensured before the emergence of SIS. In
addition, the existence of an effective monitoring and surveillance system is crucial.
Serbia is a country free from FMD. Every year, for the purpose of confirming the free
status, surveillance is carried out by serological testing of a representative number of samples, Legislatively, SIŠ is regulated by the Ruleboook on establishing measures for
early detection, diagnosis, prevention of the spread, suppression and eradication of
foot-and-mouth disease ("Official Gazette of the RS", no. 15/2010) and the Rulebook
on establishing a crisis management plan (" Official Gazette of the RS", No. 90/2015)",
publisher = "Beograd : Srpsko veterinarsko društvo",
journal = "34. Savetovanje veterinara Srbije, Zlatibor, 7-10. septembar 2023",
title = "Slinavka i šap - bolest koja nam stalno preti, Foot and mouth disease - constantly threatening disease",
pages = "20-16"
}

Milićević, V., Kureljušić, B., Glišić, D., Milovanović, B.,& Veljović, L.. (2023). Slinavka i šap - bolest koja nam stalno preti. in 34. Savetovanje veterinara Srbije, Zlatibor, 7-10. septembar 2023
Beograd : Srpsko veterinarsko društvo., 16-20.

Milićević V, Kureljušić B, Glišić D, Milovanović B, Veljović L. Slinavka i šap - bolest koja nam stalno preti. in 34. Savetovanje veterinara Srbije, Zlatibor, 7-10. septembar 2023. 2023;:16-20..

Milićević, Vesna, Kureljušić, Branislav, Glišić, Dimitrije, Milovanović, Bojan, Veljović, Ljubiša, "Slinavka i šap - bolest koja nam stalno preti" in 34. Savetovanje veterinara Srbije, Zlatibor, 7-10. septembar 2023 (2023):16-20.

TY  - CONF
AU  - Radosavljevic, Vladimir
AU  - Glišić, Dimitrije
AU  - Radanović, Oliver
AU  - Zdravković, Nemanja
AU  - Maksimović Zorić, Jelena
AU  - Maletić, Jelena
AU  - Veljović, Ljubiša
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/562
AB  - Česta pojava bolesti predstavlja jednu od najznačajnijih prepreka u obezbeđivanju daljeg rasta i održivosti akvakulture, pa su svi učsnici u akvakulturi postali
svesni koliko je proizvodnja ranjiva na namerno ili nenamerno unošenje bolesti i
značaj posledica širenja bolesti na proizvodnju i promet proizvoda akvakulture.
Shodno tome, više zaraznih bolesti je identifikovano kao prioritet za delovanje na
nacionalnom, regionalnom ili međunarodnom nivou. Iz tog razloga, brojne države su razvile i nacionalne biosigurnosne strategije u akvakulturi. Podsticaj za
sprovođenje nacionalnih programa biosigurnosti u akvakulturi je i međunarodna
obaveza države da dokaže da je implementirala biosigurnosne strategije koje su
efikasne u sprečavanju pojave, kontroli i iskorenjivanju bolesti u akvakulturi i da
su ribe i njihovi proizvodi koji potiču iz akvakulture slobodne od određenih bolesti
ili patogena. Kontrola zdravlja riba u akvakulturi Srbije se vrši na osnovu nacionalnog zakonodavstva u koje su implementirani zahtevi Svetske organizacije za
zaštitu zdravlja životinja. U ovom radu je dat pregled implementacije koncepta
biosigurnosti u slatkovodnoj akvakulturi u Republici Srbiji i susednim zemljama.
AB  - Frequent occurrence of diseases represents one of the most signiϔicant obstacles
in ensuring the further growth and sustainability of aquaculture, and all participants in aquaculture production have become aware of production vulnerability
to the intentional or unintentional introduction of diseases and the importance of
the consequences of the spread of diseases on the production and trade of aquaculture products. Consequently, several infectious diseases have been identiϔied as
priorities for action at the national, regional or international level. For this reason,
numerous countries have developed national biosecurity strategies in aquaculture.
The incentive for the implementation of national biosecurity programs in aquaculture is the international obligation of the state to prove that it has implemented  biosecurity strategies that are effective in preventing the occurrence, control and
eradication of diseases in aquaculture and that ϔish and their products originating
from aquaculture are free from certain diseases or pathogens. Fish health control
in Serbian aquaculture is carried out on the basis of national legislation, which has
implemented the requirements of the World Organization for the Protection of Animal Health. This paper provides an overview of the implementation of the concept
of biosecurity in freshwater aquaculture in the Republic of Serbia and neighboring
countries.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za DDD
PB  - Beograd : Fakultet veterinarske medicine, Katedra za zoohigijenu
C3  - 34. Savetovanje Dezinfekcija, dezinsekcija i deratizacija : Jedan svet  - jedno zdravlje
T1  - Biosigurnost u akvakulturi
T1  - Biosecurity in aquaculture
EP  - 123
SP  - 118
ER  - 

@conference{
author = "Radosavljevic, Vladimir and Glišić, Dimitrije and Radanović, Oliver and Zdravković, Nemanja and Maksimović Zorić, Jelena and Maletić, Jelena and Veljović, Ljubiša",
year = "2023",
abstract = "Česta pojava bolesti predstavlja jednu od najznačajnijih prepreka u obezbeđivanju daljeg rasta i održivosti akvakulture, pa su svi učsnici u akvakulturi postali
svesni koliko je proizvodnja ranjiva na namerno ili nenamerno unošenje bolesti i
značaj posledica širenja bolesti na proizvodnju i promet proizvoda akvakulture.
Shodno tome, više zaraznih bolesti je identifikovano kao prioritet za delovanje na
nacionalnom, regionalnom ili međunarodnom nivou. Iz tog razloga, brojne države su razvile i nacionalne biosigurnosne strategije u akvakulturi. Podsticaj za
sprovođenje nacionalnih programa biosigurnosti u akvakulturi je i međunarodna
obaveza države da dokaže da je implementirala biosigurnosne strategije koje su
efikasne u sprečavanju pojave, kontroli i iskorenjivanju bolesti u akvakulturi i da
su ribe i njihovi proizvodi koji potiču iz akvakulture slobodne od određenih bolesti
ili patogena. Kontrola zdravlja riba u akvakulturi Srbije se vrši na osnovu nacionalnog zakonodavstva u koje su implementirani zahtevi Svetske organizacije za
zaštitu zdravlja životinja. U ovom radu je dat pregled implementacije koncepta
biosigurnosti u slatkovodnoj akvakulturi u Republici Srbiji i susednim zemljama., Frequent occurrence of diseases represents one of the most signiϔicant obstacles
in ensuring the further growth and sustainability of aquaculture, and all participants in aquaculture production have become aware of production vulnerability
to the intentional or unintentional introduction of diseases and the importance of
the consequences of the spread of diseases on the production and trade of aquaculture products. Consequently, several infectious diseases have been identiϔied as
priorities for action at the national, regional or international level. For this reason,
numerous countries have developed national biosecurity strategies in aquaculture.
The incentive for the implementation of national biosecurity programs in aquaculture is the international obligation of the state to prove that it has implemented  biosecurity strategies that are effective in preventing the occurrence, control and
eradication of diseases in aquaculture and that ϔish and their products originating
from aquaculture are free from certain diseases or pathogens. Fish health control
in Serbian aquaculture is carried out on the basis of national legislation, which has
implemented the requirements of the World Organization for the Protection of Animal Health. This paper provides an overview of the implementation of the concept
of biosecurity in freshwater aquaculture in the Republic of Serbia and neighboring
countries.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za DDD, Beograd : Fakultet veterinarske medicine, Katedra za zoohigijenu",
journal = "34. Savetovanje Dezinfekcija, dezinsekcija i deratizacija : Jedan svet  - jedno zdravlje",
title = "Biosigurnost u akvakulturi, Biosecurity in aquaculture",
pages = "123-118"
}

Radosavljevic, V., Glišić, D., Radanović, O., Zdravković, N., Maksimović Zorić, J., Maletić, J.,& Veljović, L.. (2023). Biosigurnost u akvakulturi. in 34. Savetovanje Dezinfekcija, dezinsekcija i deratizacija : Jedan svet  - jedno zdravlje
Beograd : Srpsko veterinarsko društvo, Sekcija za DDD., 118-123.

Radosavljevic V, Glišić D, Radanović O, Zdravković N, Maksimović Zorić J, Maletić J, Veljović L. Biosigurnost u akvakulturi. in 34. Savetovanje Dezinfekcija, dezinsekcija i deratizacija : Jedan svet  - jedno zdravlje. 2023;:118-123..

Radosavljevic, Vladimir, Glišić, Dimitrije, Radanović, Oliver, Zdravković, Nemanja, Maksimović Zorić, Jelena, Maletić, Jelena, Veljović, Ljubiša, "Biosigurnost u akvakulturi" in 34. Savetovanje Dezinfekcija, dezinsekcija i deratizacija : Jedan svet  - jedno zdravlje (2023):118-123.

TY  - CONF
AU  - Veljović, Ljubiša
AU  - Glišić, Dimitrije
AU  - Maksimović Zorić, Jelena
AU  - Radosavljevic, Vladimir
AU  - Milićević, Vesna
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/543
AB  - Godinama se vode rasprave o pravilnoj upotrebi i razlici u definiciji izraza
efikasnost i efektivnost vakcina. Dok prva podrazumeva procenu uspešnosti
vakcine u kontrolisanim (laboratorijskim) uslovima, efektivnost vakcine se
odnosi na procenu uspešnosti vakcinacije u terenskim nekontrolisanim
uslovima nakon njene primene. Postoji čitav niz faktora koji utiču na
efikasnost i efektivnost vakcine i oni se mogu svrstati u minimalno osam
grupa. Iako efikasnost i efektivnost vakcine nisu uvek upravno srazmerni na
oba parametra mogu uticati osobine same vakcine, način primene,
karakteristike primaoca, vreme aplikacije u odnosu na pacijenta i okruženje,
prisustvo patogena u prirodi, genetski diverzitet specifičnog patogena,
način procene direktnih i indirektnih ciljeva vakcinacije kao i primenjeni
dizajn obrade podataka sa parametrima koji se prate u cilju finalne procene
efikasnosti i efektivnosti vakcine. Različiti uticaji i kombinacija više
faktora može dovesti do značajnih razlika i skoro potpunog dispariteta
efektivnosti i već dokazane visoke efikasnosti registrovanih vakcina kako
u humanoj tako i u veterinarskoj medicini. Naš cilj je da razmotrimo što veći
broj faktora, značaj i način njihovog uticaja na efikasnost i efektivnost
vakcine u slučajevima „lege artis“ primene u skladu sa uputstvom već
registrovanih vakcina za upotrebu u veterinarskoj medicini.
AB  - For years there have been discussions about the correct use and difference in definition
of the terms efficacy and effectiveness of vaccines. While the first implies the
evaluation of the success of the vaccine in controlled (laboratory) conditions, the
effectiveness of the vaccine refers to the evaluation of the success of the vaccine in
uncontrolled field conditions after its application. There are a number of factors that
affect the effectiveness and efficacy of a vaccine, and they can be classified into at
least eight groups. Although the efficacy and effectiveness of a vaccine are not always
directly proportional, both assessments can be influenced by the characteristics of the
vaccine model, vaccine application, characteristics of the recipient, time of application
in relation to the patient and the environment, the presence of pathogens in nature, the
genetic diversity of specific pathogens, the way of assessing direct and indirect targets
of vaccination as well as applied data processing design with parameters monitored
for final assessment of vaccine efficacy and effectiveness. The different influence and
combination of several factors can lead to significant differences in the assessment of
vaccine effectiveness and an almost complete discrepancy between effectiveness and
already proven high efficacy of registered vaccines, in human and veterinary medicine
as well. Our goal is to consider as many factors as possible that constantly affect the
efficacy and effectiveness of the vaccine in a positive and negative sense, in cases of
“lege artis” application according to instruction for use of already registered vaccines
in veterinary medicine.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani)
T1  - Faktori koji utiču na efikasnost i efektivnost vakcina u veterinarskoj medicini
T1  - Factors affecting the efficacy and effectiveness of vaccines in veterinary medicine
EP  - 103
SP  - 102
ER  - 

@conference{
author = "Veljović, Ljubiša and Glišić, Dimitrije and Maksimović Zorić, Jelena and Radosavljevic, Vladimir and Milićević, Vesna",
year = "2023",
abstract = "Godinama se vode rasprave o pravilnoj upotrebi i razlici u definiciji izraza
efikasnost i efektivnost vakcina. Dok prva podrazumeva procenu uspešnosti
vakcine u kontrolisanim (laboratorijskim) uslovima, efektivnost vakcine se
odnosi na procenu uspešnosti vakcinacije u terenskim nekontrolisanim
uslovima nakon njene primene. Postoji čitav niz faktora koji utiču na
efikasnost i efektivnost vakcine i oni se mogu svrstati u minimalno osam
grupa. Iako efikasnost i efektivnost vakcine nisu uvek upravno srazmerni na
oba parametra mogu uticati osobine same vakcine, način primene,
karakteristike primaoca, vreme aplikacije u odnosu na pacijenta i okruženje,
prisustvo patogena u prirodi, genetski diverzitet specifičnog patogena,
način procene direktnih i indirektnih ciljeva vakcinacije kao i primenjeni
dizajn obrade podataka sa parametrima koji se prate u cilju finalne procene
efikasnosti i efektivnosti vakcine. Različiti uticaji i kombinacija više
faktora može dovesti do značajnih razlika i skoro potpunog dispariteta
efektivnosti i već dokazane visoke efikasnosti registrovanih vakcina kako
u humanoj tako i u veterinarskoj medicini. Naš cilj je da razmotrimo što veći
broj faktora, značaj i način njihovog uticaja na efikasnost i efektivnost
vakcine u slučajevima „lege artis“ primene u skladu sa uputstvom već
registrovanih vakcina za upotrebu u veterinarskoj medicini., For years there have been discussions about the correct use and difference in definition
of the terms efficacy and effectiveness of vaccines. While the first implies the
evaluation of the success of the vaccine in controlled (laboratory) conditions, the
effectiveness of the vaccine refers to the evaluation of the success of the vaccine in
uncontrolled field conditions after its application. There are a number of factors that
affect the effectiveness and efficacy of a vaccine, and they can be classified into at
least eight groups. Although the efficacy and effectiveness of a vaccine are not always
directly proportional, both assessments can be influenced by the characteristics of the
vaccine model, vaccine application, characteristics of the recipient, time of application
in relation to the patient and the environment, the presence of pathogens in nature, the
genetic diversity of specific pathogens, the way of assessing direct and indirect targets
of vaccination as well as applied data processing design with parameters monitored
for final assessment of vaccine efficacy and effectiveness. The different influence and
combination of several factors can lead to significant differences in the assessment of
vaccine effectiveness and an almost complete discrepancy between effectiveness and
already proven high efficacy of registered vaccines, in human and veterinary medicine
as well. Our goal is to consider as many factors as possible that constantly affect the
efficacy and effectiveness of the vaccine in a positive and negative sense, in cases of
“lege artis” application according to instruction for use of already registered vaccines
in veterinary medicine.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani)",
title = "Faktori koji utiču na efikasnost i efektivnost vakcina u veterinarskoj medicini, Factors affecting the efficacy and effectiveness of vaccines in veterinary medicine",
pages = "103-102"
}

Veljović, L., Glišić, D., Maksimović Zorić, J., Radosavljevic, V.,& Milićević, V.. (2023). Faktori koji utiču na efikasnost i efektivnost vakcina u veterinarskoj medicini. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani)
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 102-103.

Veljović L, Glišić D, Maksimović Zorić J, Radosavljevic V, Milićević V. Faktori koji utiču na efikasnost i efektivnost vakcina u veterinarskoj medicini. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani). 2023;:102-103..

Veljović, Ljubiša, Glišić, Dimitrije, Maksimović Zorić, Jelena, Radosavljevic, Vladimir, Milićević, Vesna, "Faktori koji utiču na efikasnost i efektivnost vakcina u veterinarskoj medicini" in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani) (2023):102-103.

TY  - CONF
AU  - Maksimović Zorić, Jelena
AU  - Veljović, Ljubiša
AU  - Glišić, Dimitrije
AU  - Jezdimirović, Nemanja
AU  - Savić, Božidar
AU  - Milovanović, Bojan
AU  - Maletić, Jelena
AU  - Radosavljević, Vladimir
AU  - Kureljušić, Branislav
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/652
AB  - Swine influenza is a worldwide present respiratory disease of swine, in majority of cases caused by H1N1,
H1N2, and H3N2 subtypes of swine influenza A virus (swIAV). Exploration of the swIAV in Serbia in recent
ten years mainly was oriented to the passive surveillance and examination within PRDC on commercial pig
farms. The latest results suggest circulation of the viruses in the majority of the analysed commercial farms
and the presence of two subtypes - H1N1 and H3N2. Even though the problem exists none of the farms
apply vaccination as a measure of disease control. The aim of this research is to analyse the results of
passive surveillance on the two commercial farms conducted from 2020th to 2022nd.
A total of 94 samples for molecular analyses and 528 samples for serological analyses were submitted to
the Institute of Veterinary Medicine of Serbia during this period. These samples were obtained from swine
raised in two commercial farms (farm A and farm B) that practice farrow-to-finish production and have
implemented satisfactory biosecurity measures. The samples for molecular examination were mummified
fetuses, nasal swabs, oral fluids, parenchymatous organs, lungs, and sera. All samples were tested by M
gene real-time RT-PCR, according to the accredited WHO protocol (2009) for detection of influenza A
viruses. One virus was typed using multiplex real-time RT-PCR protocols described by Henritzi et al. (2016).
Serological examination was conducted using ELISA test (Ingezim Influenza A Kit, Ingenasa), and tested sera
originated from sows, suckling piglets, and nursing pigs.
During three year period influenza A viruses were detected on both farms, having on average 55,3% of the
analyzed samples positive. The highest detection rate was amongst the nasal swab (78.7%) and oral fluid
samples (83.3%), while virus was not confirmed in mummified fetuses and sera. Most of the samples
analyzed by PCR originated from farm A (80 samples), and the majority of tested were positive (60%).
Molecular typing revealed presence of H1N1 subtype in farm A that is in accordance with previous results
that confirmed H1N1 as dominating subtype that circulates in commercial farms in Serbia. Serology
examination confirmed a high overall seroprevalence of 64,6%. The serum samples from nursing pigs
accounted for the highest proportion (95.4%) among those examined, as respiratory issues linked to PRDC
typically manifest in this group. Given the endemic presence of swIAVs in the farms under analysis, there is
a pressing need to conduct further research on the circulating viruses and enhance the control measures
employed on these farms.
PB  - ESFLU
C3  - ESFLU Scientific meeting, Barcelona, 25-27th April 2023
T1  - Serological and molecular investigation of influenza in commercial pig farms in Serbia
ER  - 

@conference{
author = "Maksimović Zorić, Jelena and Veljović, Ljubiša and Glišić, Dimitrije and Jezdimirović, Nemanja and Savić, Božidar and Milovanović, Bojan and Maletić, Jelena and Radosavljević, Vladimir and Kureljušić, Branislav",
year = "2023",
abstract = "Swine influenza is a worldwide present respiratory disease of swine, in majority of cases caused by H1N1,
H1N2, and H3N2 subtypes of swine influenza A virus (swIAV). Exploration of the swIAV in Serbia in recent
ten years mainly was oriented to the passive surveillance and examination within PRDC on commercial pig
farms. The latest results suggest circulation of the viruses in the majority of the analysed commercial farms
and the presence of two subtypes - H1N1 and H3N2. Even though the problem exists none of the farms
apply vaccination as a measure of disease control. The aim of this research is to analyse the results of
passive surveillance on the two commercial farms conducted from 2020th to 2022nd.
A total of 94 samples for molecular analyses and 528 samples for serological analyses were submitted to
the Institute of Veterinary Medicine of Serbia during this period. These samples were obtained from swine
raised in two commercial farms (farm A and farm B) that practice farrow-to-finish production and have
implemented satisfactory biosecurity measures. The samples for molecular examination were mummified
fetuses, nasal swabs, oral fluids, parenchymatous organs, lungs, and sera. All samples were tested by M
gene real-time RT-PCR, according to the accredited WHO protocol (2009) for detection of influenza A
viruses. One virus was typed using multiplex real-time RT-PCR protocols described by Henritzi et al. (2016).
Serological examination was conducted using ELISA test (Ingezim Influenza A Kit, Ingenasa), and tested sera
originated from sows, suckling piglets, and nursing pigs.
During three year period influenza A viruses were detected on both farms, having on average 55,3% of the
analyzed samples positive. The highest detection rate was amongst the nasal swab (78.7%) and oral fluid
samples (83.3%), while virus was not confirmed in mummified fetuses and sera. Most of the samples
analyzed by PCR originated from farm A (80 samples), and the majority of tested were positive (60%).
Molecular typing revealed presence of H1N1 subtype in farm A that is in accordance with previous results
that confirmed H1N1 as dominating subtype that circulates in commercial farms in Serbia. Serology
examination confirmed a high overall seroprevalence of 64,6%. The serum samples from nursing pigs
accounted for the highest proportion (95.4%) among those examined, as respiratory issues linked to PRDC
typically manifest in this group. Given the endemic presence of swIAVs in the farms under analysis, there is
a pressing need to conduct further research on the circulating viruses and enhance the control measures
employed on these farms.",
publisher = "ESFLU",
journal = "ESFLU Scientific meeting, Barcelona, 25-27th April 2023",
title = "Serological and molecular investigation of influenza in commercial pig farms in Serbia"
}

Maksimović Zorić, J., Veljović, L., Glišić, D., Jezdimirović, N., Savić, B., Milovanović, B., Maletić, J., Radosavljević, V.,& Kureljušić, B.. (2023). Serological and molecular investigation of influenza in commercial pig farms in Serbia. in ESFLU Scientific meeting, Barcelona, 25-27th April 2023
ESFLU..

Maksimović Zorić J, Veljović L, Glišić D, Jezdimirović N, Savić B, Milovanović B, Maletić J, Radosavljević V, Kureljušić B. Serological and molecular investigation of influenza in commercial pig farms in Serbia. in ESFLU Scientific meeting, Barcelona, 25-27th April 2023. 2023;..

Maksimović Zorić, Jelena, Veljović, Ljubiša, Glišić, Dimitrije, Jezdimirović, Nemanja, Savić, Božidar, Milovanović, Bojan, Maletić, Jelena, Radosavljević, Vladimir, Kureljušić, Branislav, "Serological and molecular investigation of influenza in commercial pig farms in Serbia" in ESFLU Scientific meeting, Barcelona, 25-27th April 2023 (2023).

TY  - CONF
AU  - Glišić, Dimitrije
AU  - Veljović, Ljubiša
AU  - Maletić, Jelena
AU  - Kureljušić, Branislav
AU  - Ninković, Milan
AU  - Milovanović, Bojan
AU  - Milićević, Vesna
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/540
AB  - Mnoge zarazne bolesti se prenose vazduhom. Jedinke koje pokazuju kliničke
simptome respiratornih bolesti izlučuju uzročnika u sekretu, zatim
iskašljavanjem u vidu kapljica uzročnici dospevaju u spoljašnju sredinu u
kojoj mogu opstati zaštićeni unutar kapljica. Crevni patogeni se takođe mogu
širiti putem vazduha uz pomoć čestica prašine koje se podižu aktivnim
kretanjem životinja i osoblja na farmi. Neinvazivne metode uzorkovanja su
vrlo značajne za brz i jednostavan uvid u zdravstveno stanje zapata,
isključujući individualne varijacije između jedinki, i ujedno neometajući
redovne aktivnosti na farmi. Ranije je pokazano da je oralna tečnost kao uzorak
dobijen neinvazivnim putem veoma pouzdan za utvrđivanje statusa zapata.
Međutim, u našoj studiji, ispitali smo prisustvo virusnih patogena svinja u
vazduhu, uključujući virus influence svinja, cirkovirus tip dva svinja kao i
virus respiratornog i reproduktivnog sindroma svinja. Vazduh je uzorkovan
upotrebom „Aerokolekt“ uređaja. Princip rada „Aerokolekt“ uređaja je
zasnovan na strujanju vazduha kroz jednokratan čip iz kog se u laboratoriji
direktnom elucijom dobija uzorak nukleinske kiseline pogodan za PCR
analize. Uzorci su sakupljeni sa 5 farmi svinja koje imaju potvrđeno prisustvo
navedenih virusnih patogena. Rezultati ispitivanja vazduha su poređeni sa
rezultatima dobijenim iz uobičajeno sakupljenih uzoraka. Preliminarnim
rezultatima je utvrđeno da je ispitivanje vazduha pogodno za utvrđivanje
prisustva navedenih uzročnika na farmi. Primenom metodologije uzorkovanja
vazduha, može se vršiti aktivan nadzor na prisustvo patogena, održavanje
slobodnog statusa od bolesti, kao i rana detekcija patogena. Značajne
prednosti ispitivanja vazduha su i očuvanje dobrobiti, usled izostanka
direktnog kontakta sa životinjama.
AB  - Many infectious diseases are transmitted by air. Individuals which show clinical signs
of respiratory diseases excrete the culprit, and through expectoration in the form of
droplets, the causative agents end up in the external environment where they can
remain be protected within the droplets. Enteral pathogens can also be transmitted by
air, through dust particles lifted by active animal and farm staff movement. Noninvasive sampling methods are significant for swift and simple insight into the health
status of a herd, excluding individual variations, and withal not disrupting regular
farm activities. Earlier it was shown that oral fluid samples, acquired by non-invasive
methods, are a reliable way of determining the status of a herd. Meanwhile, our study
tested the presence of viral swine pathogens in the air, including the swine influenza
virus, circovirus type two, and the respiratory and reproductive syndrome virus. The
air was sampled using the “Aerocollect” device. The working principle of the
“Aerocollect” apparatus is based on the flow of air through a single-use chip from
which nucleic acid can be eluted and used for PCR analysis. The samples were
collected from five pig farms, which had a confirmed presence of the beforementioned
viral swine pathogens. The results of the analysis were compared with results from
traditionally collected samples. The preliminary results confirm that air sampling can
be used for determining the presence of the mentioned pathogens on a farm. By
applying air sampling methodology, active surveillance for the presence of pathogens
can be conducted, as well as the maintenance of free disease status, and early pathogen
detection. Considerable advantages of air sampling are the preservation of animal
welfare and the absence of direct animal contact.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani)
T1  - Virusni patogeni svinja u vazduhu
T1  - Viral swine pathogens in the air
EP  - 85
SP  - 84
ER  - 

@conference{
author = "Glišić, Dimitrije and Veljović, Ljubiša and Maletić, Jelena and Kureljušić, Branislav and Ninković, Milan and Milovanović, Bojan and Milićević, Vesna",
year = "2023",
abstract = "Mnoge zarazne bolesti se prenose vazduhom. Jedinke koje pokazuju kliničke
simptome respiratornih bolesti izlučuju uzročnika u sekretu, zatim
iskašljavanjem u vidu kapljica uzročnici dospevaju u spoljašnju sredinu u
kojoj mogu opstati zaštićeni unutar kapljica. Crevni patogeni se takođe mogu
širiti putem vazduha uz pomoć čestica prašine koje se podižu aktivnim
kretanjem životinja i osoblja na farmi. Neinvazivne metode uzorkovanja su
vrlo značajne za brz i jednostavan uvid u zdravstveno stanje zapata,
isključujući individualne varijacije između jedinki, i ujedno neometajući
redovne aktivnosti na farmi. Ranije je pokazano da je oralna tečnost kao uzorak
dobijen neinvazivnim putem veoma pouzdan za utvrđivanje statusa zapata.
Međutim, u našoj studiji, ispitali smo prisustvo virusnih patogena svinja u
vazduhu, uključujući virus influence svinja, cirkovirus tip dva svinja kao i
virus respiratornog i reproduktivnog sindroma svinja. Vazduh je uzorkovan
upotrebom „Aerokolekt“ uređaja. Princip rada „Aerokolekt“ uređaja je
zasnovan na strujanju vazduha kroz jednokratan čip iz kog se u laboratoriji
direktnom elucijom dobija uzorak nukleinske kiseline pogodan za PCR
analize. Uzorci su sakupljeni sa 5 farmi svinja koje imaju potvrđeno prisustvo
navedenih virusnih patogena. Rezultati ispitivanja vazduha su poređeni sa
rezultatima dobijenim iz uobičajeno sakupljenih uzoraka. Preliminarnim
rezultatima je utvrđeno da je ispitivanje vazduha pogodno za utvrđivanje
prisustva navedenih uzročnika na farmi. Primenom metodologije uzorkovanja
vazduha, može se vršiti aktivan nadzor na prisustvo patogena, održavanje
slobodnog statusa od bolesti, kao i rana detekcija patogena. Značajne
prednosti ispitivanja vazduha su i očuvanje dobrobiti, usled izostanka
direktnog kontakta sa životinjama., Many infectious diseases are transmitted by air. Individuals which show clinical signs
of respiratory diseases excrete the culprit, and through expectoration in the form of
droplets, the causative agents end up in the external environment where they can
remain be protected within the droplets. Enteral pathogens can also be transmitted by
air, through dust particles lifted by active animal and farm staff movement. Noninvasive sampling methods are significant for swift and simple insight into the health
status of a herd, excluding individual variations, and withal not disrupting regular
farm activities. Earlier it was shown that oral fluid samples, acquired by non-invasive
methods, are a reliable way of determining the status of a herd. Meanwhile, our study
tested the presence of viral swine pathogens in the air, including the swine influenza
virus, circovirus type two, and the respiratory and reproductive syndrome virus. The
air was sampled using the “Aerocollect” device. The working principle of the
“Aerocollect” apparatus is based on the flow of air through a single-use chip from
which nucleic acid can be eluted and used for PCR analysis. The samples were
collected from five pig farms, which had a confirmed presence of the beforementioned
viral swine pathogens. The results of the analysis were compared with results from
traditionally collected samples. The preliminary results confirm that air sampling can
be used for determining the presence of the mentioned pathogens on a farm. By
applying air sampling methodology, active surveillance for the presence of pathogens
can be conducted, as well as the maintenance of free disease status, and early pathogen
detection. Considerable advantages of air sampling are the preservation of animal
welfare and the absence of direct animal contact.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani)",
title = "Virusni patogeni svinja u vazduhu, Viral swine pathogens in the air",
pages = "85-84"
}

Glišić, D., Veljović, L., Maletić, J., Kureljušić, B., Ninković, M., Milovanović, B.,& Milićević, V.. (2023). Virusni patogeni svinja u vazduhu. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani)
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 84-85.

Glišić D, Veljović L, Maletić J, Kureljušić B, Ninković M, Milovanović B, Milićević V. Virusni patogeni svinja u vazduhu. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani). 2023;:84-85..

Glišić, Dimitrije, Veljović, Ljubiša, Maletić, Jelena, Kureljušić, Branislav, Ninković, Milan, Milovanović, Bojan, Milićević, Vesna, "Virusni patogeni svinja u vazduhu" in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani) (2023):84-85.

TY  - CONF
AU  - Maksimović Zorić, Jelena
AU  - Veljović, Ljubiša
AU  - Glišić, Dimitrije
AU  - Savić, Božidar
AU  - Jezdimirović, Nemanja
AU  - Milovanović, Bojan
AU  - Maletić, Jelena
AU  - Spalević, Ljiljana
AU  - Kureljušić, Branislav
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/505
AB  - Influenca svinja predstavlja veoma važan zdravstvni i ekonomski problem u
komercijalnom uzgoju svinja. U etiologiji respiratornih oboljenja do kojih dovode dominiraju tri podtipa virusa influence tipa A - H1N1, H1N2 i H3N2, različitih genetskih i antigenskih karakteristika (Detmer, 2017). Iako u neimunom zapatu influenca može ostvariti visok morbiditet (i do 100%), mortalitet je uglavnom nizak (manji od 1%) i zavisi od soja virusa i prisustva drugih infekcija u zapatu (Van Reeth i sar., 2012, Zell i sar., 2013). Kao posledica cirkulacije virusa na
farmi dolazi do direktnih, i mnogo značajnijih, indirektnih gubitaka koji se ogledaju u smanjenju dnevnog prirasta, povećanju utroška hrane za kilogram prirasta, produžetku tova i dr. (Došen i sar., 2008). Na ekonomičnost proizvodnje najznačajnije utiče smanjenje dnevnog prirasta (Calderón Díaz i sar., 2020), a Haden i sar., 2012, su ustanovili da gubici po grlu u slučaju monoinfekcije virusom influence iznose 3,23$. Pored direktnih i indirektnih gubitaka u
proizvodnji, cirkulacija virusa influence u populacijama svinja predstavlja i problem od javnog značaja zbog zoonotskog potencijala koji ovi virusi poseduju i mogućih uzrokovanja oboljenja ljudi. Posle pandemije 2009. godine za koju je utvrđeno da je nastala prilagođavanjem i širenjem reasortiranog virusa svinja u humanoj populaciji (Smith i sar., 2009) opisani su mnogobrojni slučajevi oboljenja ljudi nastali prenošenjem virusa sa svinja na ljude (Hennig i sar., 2022). Iako
su to najčešće incidentni slučajevi oboljenja, a tek u nekoliko navrata oboljenje manje grupe ljudi (Hennig i sar., 2022), potencijal za prenos svinjskih virusa u humanu populaciju je evidentan, što upućuje na neophodnost praćenja raširenosti ove infekcije u zapatima svinja primenom kliničke opservacije i laboratorijskih ispitivanja obolelih.
Cirkulaciju virusa i stepen raširenosti infekcije u jednom zapatu svinja u kome se ne primenjuje vakcinacija kao mera kontrole, najlakše i najekonomičnije je utvrditi primenom laboratorijskog ispitivanja prisustva specifičnih antitela protiv virusa influence tipa A. Imunološki odgovor u vidu sinteze antitela je detektabilan već trećeg dana od nastanka infekcije (Lee i sar., 1995), pri čemu pik dostiže obično između 14. i 21. dana (Radojičić i sar., 2011). Nakon primarne infekcije nastali imunitet je dugotrajan i pruža zaštitu od infekcije istim ili drugim antigeno
sličnim sojem (Van Reeth i sar., 2012). Humoralni imunološki odgovor se ogleda u sintezi specifičnih antitela protiv hemaglutinina, neuraminidaze, nukleoproteina i matriks proteina virusa (Wright i sar., 2007), a laboratorijske metode koje se koriste za njihovu detekciju su imunoenzimski testovi (ELISA), virus neutralizacioni test (VNT) i inhibibicija hemaglutinacije (IHA) (Detmer i sar., 2013). Za testiranje velikog broja uzoraka seruma koje za cilj ima utvrđivanje seroprevalencije u jednom zapatu najpogodnije je koristiti ELISA test, dok se titar
antitela kod pojedinačnih životinja kao i podtip cirkulišućih virusa utvrđuju primenom VNT ili IHA. S' obzirom na činjenicu da je cirkulacija virusa influence tipa A potvrđena na većini velikih komercijalnih farmi u Srbiji (Maksimović Zorić i sar., 2020), izvršeno je ispitivanje seroprevalencije u različitim uzrasnim kategorijama i serotipizacija cirkulišućih virusa.
PB  - Požarevac : Sitograf RM
C3  - 20. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja
T1  - Seroloska ispitivanja influence kod razlicitih uzrasnih kategorija svinja uzgajanih na komercijalnim farmama
EP  - 55
SP  - 48
ER  - 

@conference{
author = "Maksimović Zorić, Jelena and Veljović, Ljubiša and Glišić, Dimitrije and Savić, Božidar and Jezdimirović, Nemanja and Milovanović, Bojan and Maletić, Jelena and Spalević, Ljiljana and Kureljušić, Branislav",
year = "2023",
abstract = "Influenca svinja predstavlja veoma važan zdravstvni i ekonomski problem u
komercijalnom uzgoju svinja. U etiologiji respiratornih oboljenja do kojih dovode dominiraju tri podtipa virusa influence tipa A - H1N1, H1N2 i H3N2, različitih genetskih i antigenskih karakteristika (Detmer, 2017). Iako u neimunom zapatu influenca može ostvariti visok morbiditet (i do 100%), mortalitet je uglavnom nizak (manji od 1%) i zavisi od soja virusa i prisustva drugih infekcija u zapatu (Van Reeth i sar., 2012, Zell i sar., 2013). Kao posledica cirkulacije virusa na
farmi dolazi do direktnih, i mnogo značajnijih, indirektnih gubitaka koji se ogledaju u smanjenju dnevnog prirasta, povećanju utroška hrane za kilogram prirasta, produžetku tova i dr. (Došen i sar., 2008). Na ekonomičnost proizvodnje najznačajnije utiče smanjenje dnevnog prirasta (Calderón Díaz i sar., 2020), a Haden i sar., 2012, su ustanovili da gubici po grlu u slučaju monoinfekcije virusom influence iznose 3,23$. Pored direktnih i indirektnih gubitaka u
proizvodnji, cirkulacija virusa influence u populacijama svinja predstavlja i problem od javnog značaja zbog zoonotskog potencijala koji ovi virusi poseduju i mogućih uzrokovanja oboljenja ljudi. Posle pandemije 2009. godine za koju je utvrđeno da je nastala prilagođavanjem i širenjem reasortiranog virusa svinja u humanoj populaciji (Smith i sar., 2009) opisani su mnogobrojni slučajevi oboljenja ljudi nastali prenošenjem virusa sa svinja na ljude (Hennig i sar., 2022). Iako
su to najčešće incidentni slučajevi oboljenja, a tek u nekoliko navrata oboljenje manje grupe ljudi (Hennig i sar., 2022), potencijal za prenos svinjskih virusa u humanu populaciju je evidentan, što upućuje na neophodnost praćenja raširenosti ove infekcije u zapatima svinja primenom kliničke opservacije i laboratorijskih ispitivanja obolelih.
Cirkulaciju virusa i stepen raširenosti infekcije u jednom zapatu svinja u kome se ne primenjuje vakcinacija kao mera kontrole, najlakše i najekonomičnije je utvrditi primenom laboratorijskog ispitivanja prisustva specifičnih antitela protiv virusa influence tipa A. Imunološki odgovor u vidu sinteze antitela je detektabilan već trećeg dana od nastanka infekcije (Lee i sar., 1995), pri čemu pik dostiže obično između 14. i 21. dana (Radojičić i sar., 2011). Nakon primarne infekcije nastali imunitet je dugotrajan i pruža zaštitu od infekcije istim ili drugim antigeno
sličnim sojem (Van Reeth i sar., 2012). Humoralni imunološki odgovor se ogleda u sintezi specifičnih antitela protiv hemaglutinina, neuraminidaze, nukleoproteina i matriks proteina virusa (Wright i sar., 2007), a laboratorijske metode koje se koriste za njihovu detekciju su imunoenzimski testovi (ELISA), virus neutralizacioni test (VNT) i inhibibicija hemaglutinacije (IHA) (Detmer i sar., 2013). Za testiranje velikog broja uzoraka seruma koje za cilj ima utvrđivanje seroprevalencije u jednom zapatu najpogodnije je koristiti ELISA test, dok se titar
antitela kod pojedinačnih životinja kao i podtip cirkulišućih virusa utvrđuju primenom VNT ili IHA. S' obzirom na činjenicu da je cirkulacija virusa influence tipa A potvrđena na većini velikih komercijalnih farmi u Srbiji (Maksimović Zorić i sar., 2020), izvršeno je ispitivanje seroprevalencije u različitim uzrasnim kategorijama i serotipizacija cirkulišućih virusa.",
publisher = "Požarevac : Sitograf RM",
journal = "20. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja",
title = "Seroloska ispitivanja influence kod razlicitih uzrasnih kategorija svinja uzgajanih na komercijalnim farmama",
pages = "55-48"
}

Maksimović Zorić, J., Veljović, L., Glišić, D., Savić, B., Jezdimirović, N., Milovanović, B., Maletić, J., Spalević, L.,& Kureljušić, B.. (2023). Seroloska ispitivanja influence kod razlicitih uzrasnih kategorija svinja uzgajanih na komercijalnim farmama. in 20. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja
Požarevac : Sitograf RM., 48-55.

Maksimović Zorić J, Veljović L, Glišić D, Savić B, Jezdimirović N, Milovanović B, Maletić J, Spalević L, Kureljušić B. Seroloska ispitivanja influence kod razlicitih uzrasnih kategorija svinja uzgajanih na komercijalnim farmama. in 20. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja. 2023;:48-55..

Maksimović Zorić, Jelena, Veljović, Ljubiša, Glišić, Dimitrije, Savić, Božidar, Jezdimirović, Nemanja, Milovanović, Bojan, Maletić, Jelena, Spalević, Ljiljana, Kureljušić, Branislav, "Seroloska ispitivanja influence kod razlicitih uzrasnih kategorija svinja uzgajanih na komercijalnim farmama" in 20. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja (2023):48-55.

TY  - JOUR
AU  - Glišić, Dimitrije
AU  - Milićević, Vesna
AU  - Veljović, Ljubiša
AU  - Milovanović, Bojan
AU  - Kureljušić, Branislav
AU  - Đorđević, Igor
AU  - Anđelković, Katarina
AU  - Petković, Jelena
AU  - Dacić, Miroljub
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/492
AB  - The first case of African swine fever in domestic pigs in Serbia was in 2019. The following year, the disease was confirmed in wild boar. Thenceforth, ASF has been continuously reported in both wild and domestic pigs. The outbreaks in domestic pigs could not be linked directly to wild boars, even though wild boars were endemically infected, and reservoirs for ASF. This study aimed to investigate outbreaks and routes of transmission in domestic pigs in a region of central Serbia where no outbreaks in wild boar were reported. Fourteen outbreaks of ASF on backyard farms with low biosecurity were traced back, and no connection to wild boar was found. The epidemic investigation covered 2094 holdings, with 24,368 pigs, out of which 1882 were tested for ASF. In surrounding hunting grounds, field searches were conducted. Dead wild boars were found, and 138 hunted wild boars were negative for ASFV. It was concluded that outbreaks in 2021 were provoked by the illegal trade of live animals and pig products. Even though infective pressure from wild boars is assumed, no positive cases have been found, while the ASFV spreads within the domestic swine population evidenced in four recent outbreaks in 2022.
PB  - MDPI, Basel
T2  - Pathogens
T1  - Patterns of ASFV Transmission in Domestic Pigs in Serbia
IS  - 1
VL  - 12
DO  - 10.3390/pathogens12010149
UR  - conv_601
ER  - 

@article{
author = "Glišić, Dimitrije and Milićević, Vesna and Veljović, Ljubiša and Milovanović, Bojan and Kureljušić, Branislav and Đorđević, Igor and Anđelković, Katarina and Petković, Jelena and Dacić, Miroljub",
year = "2023",
abstract = "The first case of African swine fever in domestic pigs in Serbia was in 2019. The following year, the disease was confirmed in wild boar. Thenceforth, ASF has been continuously reported in both wild and domestic pigs. The outbreaks in domestic pigs could not be linked directly to wild boars, even though wild boars were endemically infected, and reservoirs for ASF. This study aimed to investigate outbreaks and routes of transmission in domestic pigs in a region of central Serbia where no outbreaks in wild boar were reported. Fourteen outbreaks of ASF on backyard farms with low biosecurity were traced back, and no connection to wild boar was found. The epidemic investigation covered 2094 holdings, with 24,368 pigs, out of which 1882 were tested for ASF. In surrounding hunting grounds, field searches were conducted. Dead wild boars were found, and 138 hunted wild boars were negative for ASFV. It was concluded that outbreaks in 2021 were provoked by the illegal trade of live animals and pig products. Even though infective pressure from wild boars is assumed, no positive cases have been found, while the ASFV spreads within the domestic swine population evidenced in four recent outbreaks in 2022.",
publisher = "MDPI, Basel",
journal = "Pathogens",
title = "Patterns of ASFV Transmission in Domestic Pigs in Serbia",
number = "1",
volume = "12",
doi = "10.3390/pathogens12010149",
url = "conv_601"
}

Glišić, D., Milićević, V., Veljović, L., Milovanović, B., Kureljušić, B., Đorđević, I., Anđelković, K., Petković, J.,& Dacić, M.. (2023). Patterns of ASFV Transmission in Domestic Pigs in Serbia. in Pathogens
MDPI, Basel., 12(1).
https://doi.org/10.3390/pathogens12010149
conv_601

Glišić D, Milićević V, Veljović L, Milovanović B, Kureljušić B, Đorđević I, Anđelković K, Petković J, Dacić M. Patterns of ASFV Transmission in Domestic Pigs in Serbia. in Pathogens. 2023;12(1).
doi:10.3390/pathogens12010149
conv_601 .

Glišić, Dimitrije, Milićević, Vesna, Veljović, Ljubiša, Milovanović, Bojan, Kureljušić, Branislav, Đorđević, Igor, Anđelković, Katarina, Petković, Jelena, Dacić, Miroljub, "Patterns of ASFV Transmission in Domestic Pigs in Serbia" in Pathogens, 12, no. 1 (2023),
https://doi.org/10.3390/pathogens12010149 .,
conv_601 .

TY  - JOUR
AU  - Milićević, Vesna
AU  - Glišić, Dimitrije
AU  - Zurovac-Sapundžić, Zorana
AU  - Ninković, Milan
AU  - Milovanović, Bojan
AU  - Veljović, Ljubiša
AU  - Kureljušić, Branislav
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/490
AB  - Canine parvovirus 2 (CPV-2) is the causal agent of canine parvovirosis an infectious disease with the high fatality rate among dogs. However, in Serbia, it has never been investigated thoroughly. This study was conducted on samples collected from dogs with diarrhea in anamnesis, submitted for various reasons to the Institute of Veterinary Medicine of Serbia, and stored in the sample bank. In total, 50 rectal swab samples were collected from the period 2008 to 2020, and consequently tested. Out of 50 rectal swab samples, the CPV-2 genome was detected in 14 (28%). This retrospective study showed the presence of three different subtypes of CPV-2 in diarrheic dogs during the last 12 years in Serbia. CPV-2a was the most prevalent subtype (60%) followed by CPV-2b (30%), and CPV-2c (10%). Interestingly, CPV-2a had been the predominantly detected subtype up until 2018. Nevertheless in 2019, there was the first detected occurrence of the CPV-2b, followed by the first detection of the CPV-2c in 2020. This study reports the evidence and distribution of CPV-2 from 2008 to 2020, providing new information about the presence of virus strains in Serbia.
PB  - Springer, Dordrecht
T2  - Veterinary Research Communications
T1  - Molecular characterization of Canine parvovirus type 2 from diarrheic dogs in Serbia from 2008 to 2020
EP  - 289
IS  - 1
SP  - 285
VL  - 47
DO  - 10.1007/s11259-022-09924-5
UR  - conv_585
ER  - 

@article{
author = "Milićević, Vesna and Glišić, Dimitrije and Zurovac-Sapundžić, Zorana and Ninković, Milan and Milovanović, Bojan and Veljović, Ljubiša and Kureljušić, Branislav",
year = "2023",
abstract = "Canine parvovirus 2 (CPV-2) is the causal agent of canine parvovirosis an infectious disease with the high fatality rate among dogs. However, in Serbia, it has never been investigated thoroughly. This study was conducted on samples collected from dogs with diarrhea in anamnesis, submitted for various reasons to the Institute of Veterinary Medicine of Serbia, and stored in the sample bank. In total, 50 rectal swab samples were collected from the period 2008 to 2020, and consequently tested. Out of 50 rectal swab samples, the CPV-2 genome was detected in 14 (28%). This retrospective study showed the presence of three different subtypes of CPV-2 in diarrheic dogs during the last 12 years in Serbia. CPV-2a was the most prevalent subtype (60%) followed by CPV-2b (30%), and CPV-2c (10%). Interestingly, CPV-2a had been the predominantly detected subtype up until 2018. Nevertheless in 2019, there was the first detected occurrence of the CPV-2b, followed by the first detection of the CPV-2c in 2020. This study reports the evidence and distribution of CPV-2 from 2008 to 2020, providing new information about the presence of virus strains in Serbia.",
publisher = "Springer, Dordrecht",
journal = "Veterinary Research Communications",
title = "Molecular characterization of Canine parvovirus type 2 from diarrheic dogs in Serbia from 2008 to 2020",
pages = "289-285",
number = "1",
volume = "47",
doi = "10.1007/s11259-022-09924-5",
url = "conv_585"
}

Milićević, V., Glišić, D., Zurovac-Sapundžić, Z., Ninković, M., Milovanović, B., Veljović, L.,& Kureljušić, B.. (2023). Molecular characterization of Canine parvovirus type 2 from diarrheic dogs in Serbia from 2008 to 2020. in Veterinary Research Communications
Springer, Dordrecht., 47(1), 285-289.
https://doi.org/10.1007/s11259-022-09924-5
conv_585

Milićević V, Glišić D, Zurovac-Sapundžić Z, Ninković M, Milovanović B, Veljović L, Kureljušić B. Molecular characterization of Canine parvovirus type 2 from diarrheic dogs in Serbia from 2008 to 2020. in Veterinary Research Communications. 2023;47(1):285-289.
doi:10.1007/s11259-022-09924-5
conv_585 .

Milićević, Vesna, Glišić, Dimitrije, Zurovac-Sapundžić, Zorana, Ninković, Milan, Milovanović, Bojan, Veljović, Ljubiša, Kureljušić, Branislav, "Molecular characterization of Canine parvovirus type 2 from diarrheic dogs in Serbia from 2008 to 2020" in Veterinary Research Communications, 47, no. 1 (2023):285-289,
https://doi.org/10.1007/s11259-022-09924-5 .,
conv_585 .

TY  - CONF
AU  - Petrović, Tamaš
AU  - Šekler, Milanko
AU  - Debeljak, Zoran
AU  - Petric, Dušan
AU  - Labus, Tatjana
AU  - Matović, Kazimir
AU  - Tešović, Bojana
AU  - Veljović, Ljubiša
AU  - Kavran, Mihaela
AU  - Lupulović, Diana
AU  - Gajdov, Vladimir
AU  - Ignjatović Ćupina, Aleksandra
AU  - Kolarević, Mišo
AU  - Đurić, Boban
AU  - Petrović, Miloš
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/548
AB  - Poslednjih nekoliko godina je u Srbiji konstanto prisutna cirkulacija
virusa Zapadnog Nila (VZN). U humanoj populacije su zabeležene veće ili
manje epidemije počev od 2012. godine pa sve do danas. Veterinarska služba u
Srbiji je od juna 2022. godine pokrenula godišnji nacionalni program
monitoringa VZN, koji je predstavljao nastavak godišnjih programa
monitoringa počev od 2014. godine. Program je finansiran od strane Uprave
za veterinu, a na terenu ga je sprovodila veterinarska služba u saradnji sa
entomolozima i ornitolozima. Cilj programa je bila rana detekcija prisustva
VZN na nekom području i pravovremeno alarmiranje humane zdravstvene službe
i lokalnih samouprava radi sprovođenja mera kontrole (suzbijanja komaraca) i
informisanja stanovništva.
Program monitoringa se zasnivao na indirektnom i direktnom praćenju
prisustva VZN u prirodi. Indirektno praćenje virusa se vršilo serološkim
testiranjem konja na prisustvo IgM antitela i sentinel teladi uzrasta 6 do 8
meseci (oteljenih nakon sezone vektora u prethodnoj godini) na IgG antitela
protiv VZN kao potvrda akutne infekcije i cirkulacije VZN. Direktno
praćenje se vršilo ispitivanjima zbirnih uzoraka komaraca i uzoraka divljih
ptica na prisustvo VZN molekularnim dijagnostičkim metodama u aktivnom i
uzoraka na bolest suspektnih konja u pasivnom nadzoru. Broj uzoraka za
ispitivanje je određen po okruzima na osnovu visine rizika od pojave
infekcije VZN.
U periodu jun-septembar 2022. na području R. Srbije je po programu ispitano
1853 krvnih seruma konja od čega je kod 25 (1,30%) utvrđeno prisustvo IgM
antitela. Ista je bila u uzlaznoj liniji sa povećanjem aktivnosti vektora i u
junu je kod 0,9% (4/437), u julu kod 2,0% (10/490), u avgustu kod 1,9% (9/468) i u
septembru kod 0,9% (4/458) konja utvrđen pozitivan serološki odgovor. Broj
okruga u kojima je detektovana serokonverzija je iznosio 2 u junu, 3 u julu, 5 u 
avgustu i 3 u septembru. U istom periodu je na području visoko rizičnih okruga
(14/25) kod 1740 ispitanih teladi utvrđeno prisustvo IgG antitela protiv
VZN kod 8,0% životinja (140/1740), odnosno kod 11 (2,7%), 34 (6, 9%), 46
(10,6%) i 49 (11,8%) sentinel teladi, na području 4/14, 7/14, 9/14 i 9/14 okruga
u junu, julu, avgustu i septembru
U direktnom praćenju prisustva VZN ispitano je 792 zbirnih uzoraka (pulova)
komaraca i VZN je potvrđen u 34 (4,3%) uzorka. Prevalencija VZN u komarcima
je rasla od negativnog nalaza u junu (0/160), prvog pozitivnog nalaza 5,9%
(19/320) u julu, pozitivnih nalaza 4,5% (7/156) u avgustu i 5,1% (8/156) u
septembru. Pozitivni uzorci komaraca na VZN su detektovani u 9 od 25 okruga:
> 20% pozitivnih od ispitanih uzoraka je utvrđeno na području Beograda,
19,15% u Srednjebanatskom, 18,37% u Južnobanatskom, 8,7% u Sremskom, 8,33%
Južnobačkom, 6,0% u Šumadijskom, 4,17% u Severnobanatskom, 4,08% u
Severnobačkom i 2.0% u Zapadnobačkom okrugu. VZN je utvrđen u uzorcima
11/52 (21,1%) uginulih divljih ptica sa područja 7 okruga, kao i u 14 (3,7%) od
379 ispitanih uzoraka ždrelnih briseva živih divljih ptica na području 4
okruga.
Program monitoringa VZN se tokom 2022. godine pokazao kao uspešan i
svrsishodan i neophodno ga je nastaviti i unaprediti u narednom periodu u
cilju pravovremene zaštite zdravlja ljudi i životinja.
AB  - The constant circulation of West Nile virus (WNV) has been detected in the last few
years in Serbia. Bigger or smaller epidemics have been reported in human population
in Serbia since 2012, until today. The veterinary service in Serbia launched the yearly
national program for WNV monitoring in June 2022, and it was a continuation of the
yearly monitoring programs conducted since 2014. The program was funded by the
Veterinary Directorate, and it is implemented on the field by veterinary service in
collaboration with entomologists and ornithologists. The main objective of the
monitoring program was the early detection of the presence of WNV in a certain area,
and timely alerting of human health services and local governments in order to control
the mosquito population and to inform the local communities.
The monitoring program was based on the direct and indirect monitoring of the
presence of WNV in the environment. Indirect monitoring of virus presence was
performed by serological testing of horses on the presence of anti-WNV IgM
antibodies, and sentinel calves aged 6 to 8 months (calved after the vector season in
the previous year) for the presence of anti-WNV IgG antibodies, as the confirmation
of acute infection. Direct monitoring of the WNV presence in nature was done by
molecular testing of WNV presence in pooled mosquito’s samples and in wild birds
in active surveillance and by testing the samples of clinically suspected horses in
passive surveillance. The number of tested samples was defined at the level of each
district of the Republic of Serbia in relation to the risks of WNV infection.
In the period of June-September 2022 in Serbia, according to the program, 1853 blood
sera of horses were tested, and seroconversion (IgM antibodies) was detected in 25
(1.3%) horses. The seroconversion was in an upward line in concordance with the
increase in the activity of the vectors, thus positive serological responses were
determined in June in 0.9% (4/437), in July in 2.0% (10/490), in August in 1.9%
(9/468), and in September in 0.9% (4/458) of tested horses. The number of districts
where the positive horses were detected was: 2 in June, 3 in July, 5 in August and 3
in September. In the same period, in high-risk districts (14/25), in 1740 examined
calves, the presence of IgG-anti-WNV antibodies was determined in 8.0% of animals (140/1740), i.e. in 11 (2.7%), 34 (6, 9%), 46 (10.6%) and 49 (11.8%) sentinel calves,
in the 4/14, 7/14, 9/14 and 9/14 districts in June, July, August and September.
In direct monitoring of virus presence, 792 pooled mosquito samples were tested from
June to September 2022, and WNV was confirmed in 34 (4.3%) samples. The
prevalence of WNV in mosquitoes increased from a negative finding in June (0/160),
to the first positive finding 5.9% (19/320) in July, and positive findings 4.5% (7/156)
in August and 5.1% (8/156) in September. Positive mosquito samples were detected
in 9 out of 25 districts of Serbia: > 20% positive out of the tested samples were
detected in the Belgrade area, 19.15% in the Central Banat, 18.37% in the South
Banat, 8.7% in the Srem, 8.33% in the South Bačka, 6.0% in the Šumadia, 4.17% in
North Banat, 4.08% in North Bačka and 2.0% in West Bačka district. WNV was
determined in 11 samples out of 52 (21.1%) dead wild birds from the area of 7
districts, as well as in 14 (3.7%) out of 379 examined samples of pharyngeal swabs of
live captured wild birds in the area of 4 districts.
WNV monitoring program during 2022 proved to be successful and meaningful and
it is necessary to continue and improve it in the forthcoming period in order to ensure
the timely protection of human and animal health.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani)
T1  - Rezultati programa monitoringa bolesti Zapadnog Nila Republici Srbiji u 2022. godini
T1  - Results of WNV monitoring program in Serbia in 2022
EP  - 161
SP  - 158
ER  - 

@conference{
author = "Petrović, Tamaš and Šekler, Milanko and Debeljak, Zoran and Petric, Dušan and Labus, Tatjana and Matović, Kazimir and Tešović, Bojana and Veljović, Ljubiša and Kavran, Mihaela and Lupulović, Diana and Gajdov, Vladimir and Ignjatović Ćupina, Aleksandra and Kolarević, Mišo and Đurić, Boban and Petrović, Miloš",
year = "2023",
abstract = "Poslednjih nekoliko godina je u Srbiji konstanto prisutna cirkulacija
virusa Zapadnog Nila (VZN). U humanoj populacije su zabeležene veće ili
manje epidemije počev od 2012. godine pa sve do danas. Veterinarska služba u
Srbiji je od juna 2022. godine pokrenula godišnji nacionalni program
monitoringa VZN, koji je predstavljao nastavak godišnjih programa
monitoringa počev od 2014. godine. Program je finansiran od strane Uprave
za veterinu, a na terenu ga je sprovodila veterinarska služba u saradnji sa
entomolozima i ornitolozima. Cilj programa je bila rana detekcija prisustva
VZN na nekom području i pravovremeno alarmiranje humane zdravstvene službe
i lokalnih samouprava radi sprovođenja mera kontrole (suzbijanja komaraca) i
informisanja stanovništva.
Program monitoringa se zasnivao na indirektnom i direktnom praćenju
prisustva VZN u prirodi. Indirektno praćenje virusa se vršilo serološkim
testiranjem konja na prisustvo IgM antitela i sentinel teladi uzrasta 6 do 8
meseci (oteljenih nakon sezone vektora u prethodnoj godini) na IgG antitela
protiv VZN kao potvrda akutne infekcije i cirkulacije VZN. Direktno
praćenje se vršilo ispitivanjima zbirnih uzoraka komaraca i uzoraka divljih
ptica na prisustvo VZN molekularnim dijagnostičkim metodama u aktivnom i
uzoraka na bolest suspektnih konja u pasivnom nadzoru. Broj uzoraka za
ispitivanje je određen po okruzima na osnovu visine rizika od pojave
infekcije VZN.
U periodu jun-septembar 2022. na području R. Srbije je po programu ispitano
1853 krvnih seruma konja od čega je kod 25 (1,30%) utvrđeno prisustvo IgM
antitela. Ista je bila u uzlaznoj liniji sa povećanjem aktivnosti vektora i u
junu je kod 0,9% (4/437), u julu kod 2,0% (10/490), u avgustu kod 1,9% (9/468) i u
septembru kod 0,9% (4/458) konja utvrđen pozitivan serološki odgovor. Broj
okruga u kojima je detektovana serokonverzija je iznosio 2 u junu, 3 u julu, 5 u 
avgustu i 3 u septembru. U istom periodu je na području visoko rizičnih okruga
(14/25) kod 1740 ispitanih teladi utvrđeno prisustvo IgG antitela protiv
VZN kod 8,0% životinja (140/1740), odnosno kod 11 (2,7%), 34 (6, 9%), 46
(10,6%) i 49 (11,8%) sentinel teladi, na području 4/14, 7/14, 9/14 i 9/14 okruga
u junu, julu, avgustu i septembru
U direktnom praćenju prisustva VZN ispitano je 792 zbirnih uzoraka (pulova)
komaraca i VZN je potvrđen u 34 (4,3%) uzorka. Prevalencija VZN u komarcima
je rasla od negativnog nalaza u junu (0/160), prvog pozitivnog nalaza 5,9%
(19/320) u julu, pozitivnih nalaza 4,5% (7/156) u avgustu i 5,1% (8/156) u
septembru. Pozitivni uzorci komaraca na VZN su detektovani u 9 od 25 okruga:
> 20% pozitivnih od ispitanih uzoraka je utvrđeno na području Beograda,
19,15% u Srednjebanatskom, 18,37% u Južnobanatskom, 8,7% u Sremskom, 8,33%
Južnobačkom, 6,0% u Šumadijskom, 4,17% u Severnobanatskom, 4,08% u
Severnobačkom i 2.0% u Zapadnobačkom okrugu. VZN je utvrđen u uzorcima
11/52 (21,1%) uginulih divljih ptica sa područja 7 okruga, kao i u 14 (3,7%) od
379 ispitanih uzoraka ždrelnih briseva živih divljih ptica na području 4
okruga.
Program monitoringa VZN se tokom 2022. godine pokazao kao uspešan i
svrsishodan i neophodno ga je nastaviti i unaprediti u narednom periodu u
cilju pravovremene zaštite zdravlja ljudi i životinja., The constant circulation of West Nile virus (WNV) has been detected in the last few
years in Serbia. Bigger or smaller epidemics have been reported in human population
in Serbia since 2012, until today. The veterinary service in Serbia launched the yearly
national program for WNV monitoring in June 2022, and it was a continuation of the
yearly monitoring programs conducted since 2014. The program was funded by the
Veterinary Directorate, and it is implemented on the field by veterinary service in
collaboration with entomologists and ornithologists. The main objective of the
monitoring program was the early detection of the presence of WNV in a certain area,
and timely alerting of human health services and local governments in order to control
the mosquito population and to inform the local communities.
The monitoring program was based on the direct and indirect monitoring of the
presence of WNV in the environment. Indirect monitoring of virus presence was
performed by serological testing of horses on the presence of anti-WNV IgM
antibodies, and sentinel calves aged 6 to 8 months (calved after the vector season in
the previous year) for the presence of anti-WNV IgG antibodies, as the confirmation
of acute infection. Direct monitoring of the WNV presence in nature was done by
molecular testing of WNV presence in pooled mosquito’s samples and in wild birds
in active surveillance and by testing the samples of clinically suspected horses in
passive surveillance. The number of tested samples was defined at the level of each
district of the Republic of Serbia in relation to the risks of WNV infection.
In the period of June-September 2022 in Serbia, according to the program, 1853 blood
sera of horses were tested, and seroconversion (IgM antibodies) was detected in 25
(1.3%) horses. The seroconversion was in an upward line in concordance with the
increase in the activity of the vectors, thus positive serological responses were
determined in June in 0.9% (4/437), in July in 2.0% (10/490), in August in 1.9%
(9/468), and in September in 0.9% (4/458) of tested horses. The number of districts
where the positive horses were detected was: 2 in June, 3 in July, 5 in August and 3
in September. In the same period, in high-risk districts (14/25), in 1740 examined
calves, the presence of IgG-anti-WNV antibodies was determined in 8.0% of animals (140/1740), i.e. in 11 (2.7%), 34 (6, 9%), 46 (10.6%) and 49 (11.8%) sentinel calves,
in the 4/14, 7/14, 9/14 and 9/14 districts in June, July, August and September.
In direct monitoring of virus presence, 792 pooled mosquito samples were tested from
June to September 2022, and WNV was confirmed in 34 (4.3%) samples. The
prevalence of WNV in mosquitoes increased from a negative finding in June (0/160),
to the first positive finding 5.9% (19/320) in July, and positive findings 4.5% (7/156)
in August and 5.1% (8/156) in September. Positive mosquito samples were detected
in 9 out of 25 districts of Serbia: > 20% positive out of the tested samples were
detected in the Belgrade area, 19.15% in the Central Banat, 18.37% in the South
Banat, 8.7% in the Srem, 8.33% in the South Bačka, 6.0% in the Šumadia, 4.17% in
North Banat, 4.08% in North Bačka and 2.0% in West Bačka district. WNV was
determined in 11 samples out of 52 (21.1%) dead wild birds from the area of 7
districts, as well as in 14 (3.7%) out of 379 examined samples of pharyngeal swabs of
live captured wild birds in the area of 4 districts.
WNV monitoring program during 2022 proved to be successful and meaningful and
it is necessary to continue and improve it in the forthcoming period in order to ensure
the timely protection of human and animal health.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani)",
title = "Rezultati programa monitoringa bolesti Zapadnog Nila Republici Srbiji u 2022. godini, Results of WNV monitoring program in Serbia in 2022",
pages = "161-158"
}

Petrović, T., Šekler, M., Debeljak, Z., Petric, D., Labus, T., Matović, K., Tešović, B., Veljović, L., Kavran, M., Lupulović, D., Gajdov, V., Ignjatović Ćupina, A., Kolarević, M., Đurić, B.,& Petrović, M.. (2023). Rezultati programa monitoringa bolesti Zapadnog Nila Republici Srbiji u 2022. godini. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani)
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 158-161.

Petrović T, Šekler M, Debeljak Z, Petric D, Labus T, Matović K, Tešović B, Veljović L, Kavran M, Lupulović D, Gajdov V, Ignjatović Ćupina A, Kolarević M, Đurić B, Petrović M. Rezultati programa monitoringa bolesti Zapadnog Nila Republici Srbiji u 2022. godini. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani). 2023;:158-161..

Petrović, Tamaš, Šekler, Milanko, Debeljak, Zoran, Petric, Dušan, Labus, Tatjana, Matović, Kazimir, Tešović, Bojana, Veljović, Ljubiša, Kavran, Mihaela, Lupulović, Diana, Gajdov, Vladimir, Ignjatović Ćupina, Aleksandra, Kolarević, Mišo, Đurić, Boban, Petrović, Miloš, "Rezultati programa monitoringa bolesti Zapadnog Nila Republici Srbiji u 2022. godini" in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani) (2023):158-161.

TY  - CONF
AU  - Maksimović Zorić, Jelena
AU  - Veljović, Ljubiša
AU  - Glišić, Dimitrije
AU  - Vojinovic, Dragica
AU  - Zurovac Sapundžić, Zorana
AU  - Ninković, Milan
AU  - Zdravković, Nemanja
AU  - Radanović, Oliver
AU  - Jezdimirović, Nemanja
AU  - Stanojević, Slobodan
AU  - Žutić, Jadranka
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/542
AB  - Pobačaj u govedarstvu predstavlja prekid graviditeta i istiskivanje nezrelog
ploda iz uterusa u periodu između 42. i 260. dana steonosti. Prema etiologiji
pobačaji se dele na infektivne i neinfektivne. Infektivni uzročnici pored
pobačaja mogu prouzrokovati i druge zdravstvene poremećaje, dovodeći na taj način
do povećanja direktnih i indirektnih ekonomskih gubitaka u proizvodnji. Neki
od njih (Brucella spp, Coxiella burnetii, Listeria monocytogenes) zbog zoonotskog
potencijala koji poseduju predstavljaju zdravstveni rizik po humanu populaciju.
Iz ovih razloga laboratorijska dijagnostika infektivnih uzročnika pobačaja kod
goveda predstavlja obavezan deo zdravstvenog nadzora populacije goveda u
Republici Srbiji, i na godišnjem nivou se propisuje kroz Pravilnik o
utvrđivanju Programa mera zdravstvene zaštite životinja. U periodu od 2019. do
2022. godine u Naučni institut za veterinarstvo Srbije je dostavljeno 118 uzoraka
krvnih seruma i 105 uzoraka patološkog materijala od pobačenih plodova od
jedinki uzgajanih na teritoriji beogradskog epizootiološkog područja. Od toga,
23 uzorka seruma i 17 uzoraka poreklom od pobačenih plodova su poreklom od
jedinki uzgajanih na komercijalnoj govedarskoj farmi, dok je preostalih 95
uzoraka seruma i 88 uzoraka od pobačenih plodova uzorkovano na gazdinstvima koja
se odlikuju ekstenzivnom proizvodnjom. Metodama direknog dokazivanja
infektivnih uzročnika u patološkom materijalu poreklom od pobačenog ploda u
dva uzorka je utvrđeno prisustvo virusa goveđe virusne dijareje, u četiri bakterije
Listeria monocytogenes i u jednom protozoe Neospora caninum. U 93,3% ispitanih
uzoraka nije dokazan nijedan od pravilnikom predviđenih infektivnih uzročnika
pobačaja. Indirektnim, serološkim metodama dijagnostike utvrđena je visoka
seroprevalencija na Neospora caninum (29,2%), dok su hlamidioza i kju-groznica
potvrđene redom u 2,5% i 10,2% ispitanih uzoraka. Serološki odgovor na virus
goveđe virusne dijareje i virus infektivnog bovinog rinotraheitisa/pustuloznog
vulvovaginitisa ustanovljen je redom kod 5,3% odnosno 4,2% ispitanih jedinki sa
individualnih gazdinstava, dok su kod životinja sa komercijalne farme usled
primene vakcinacije dobijeni procenti značajno viši (43,5% i 87%).
AB  - Abortion in cattle is defined as the termination of pregnancy and expulsion of an
immature fetus from the uterus between the 42nd and 260th day of pregnancy.
Abortions can be caused by infectious and non-infectious causative agents. Infectious
agents, besides abortion, can cause additional health disorders, leading to significant
direct and indirect economic losses. Some of them (Brucella spp, Coxiella burnetii,
Listeria monocytogenes) pose a risk to human health as well. Accordingly, laboratory
diagnostics of infectious abortions in cattle is a part of the mandatory surveillance of
cattle health in Serbia. It is dictated on an annual basis through the Legal regulation
on the Program of the Animal Health Protection Measures. In the period from 2019
to 2022, 118 sera and 105 samples of pathological material from aborted fetuses were
collected on the territory of the Belgrade epizootiological area and analyzed at the
Institute of Veterinary Medicine of Serbia. Of these, 23 serа and 17 aborted fetuses
originated from commercial cattle farms, while 95 serа and 88 aborted fetuses were
sampled on farms characterized by extensive production. By methods of direct
detection in the pathological material from fetuses bovine viral diarrhea virus was
detected in two, Listeria monocytogenes in four, and Neospora caninum in one
sample. In 93.3% of analyzed samples, none of the tested pathogens was detected.
Indirect, serological diagnostic methods revealed a high seroprevalence of Neospora
caninum (29.2%), while Chlamydiosis and Q fever were confirmed in 2.5% and
10.2% of the tested samples, respectively. A serological response to the bovine viral
diarrhoea virus and infectious bovine rhinotracheitis/pustular vulvovaginitis virus
were found in 5.3% and 4.2% of the samples from extensive production, respectively,
while due to the vaccination, percentages were significantly higher in analyzed cattle
from intensive production (43.5% and 87% respectively).
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani)
T1  - Rezultati laboratorijskih ispitivanja kod pobačaja goveda na teritoriji Beogradskog epizootiološkog područja u periodu od 2019. do 2022. godine
T1  - Results of laboratory examinations of abortions in cattle on the Belgrade epizootiological area in the period from 2019 - 2022
EP  - 97
SP  - 96
ER  - 

@conference{
author = "Maksimović Zorić, Jelena and Veljović, Ljubiša and Glišić, Dimitrije and Vojinovic, Dragica and Zurovac Sapundžić, Zorana and Ninković, Milan and Zdravković, Nemanja and Radanović, Oliver and Jezdimirović, Nemanja and Stanojević, Slobodan and Žutić, Jadranka",
year = "2023",
abstract = "Pobačaj u govedarstvu predstavlja prekid graviditeta i istiskivanje nezrelog
ploda iz uterusa u periodu između 42. i 260. dana steonosti. Prema etiologiji
pobačaji se dele na infektivne i neinfektivne. Infektivni uzročnici pored
pobačaja mogu prouzrokovati i druge zdravstvene poremećaje, dovodeći na taj način
do povećanja direktnih i indirektnih ekonomskih gubitaka u proizvodnji. Neki
od njih (Brucella spp, Coxiella burnetii, Listeria monocytogenes) zbog zoonotskog
potencijala koji poseduju predstavljaju zdravstveni rizik po humanu populaciju.
Iz ovih razloga laboratorijska dijagnostika infektivnih uzročnika pobačaja kod
goveda predstavlja obavezan deo zdravstvenog nadzora populacije goveda u
Republici Srbiji, i na godišnjem nivou se propisuje kroz Pravilnik o
utvrđivanju Programa mera zdravstvene zaštite životinja. U periodu od 2019. do
2022. godine u Naučni institut za veterinarstvo Srbije je dostavljeno 118 uzoraka
krvnih seruma i 105 uzoraka patološkog materijala od pobačenih plodova od
jedinki uzgajanih na teritoriji beogradskog epizootiološkog područja. Od toga,
23 uzorka seruma i 17 uzoraka poreklom od pobačenih plodova su poreklom od
jedinki uzgajanih na komercijalnoj govedarskoj farmi, dok je preostalih 95
uzoraka seruma i 88 uzoraka od pobačenih plodova uzorkovano na gazdinstvima koja
se odlikuju ekstenzivnom proizvodnjom. Metodama direknog dokazivanja
infektivnih uzročnika u patološkom materijalu poreklom od pobačenog ploda u
dva uzorka je utvrđeno prisustvo virusa goveđe virusne dijareje, u četiri bakterije
Listeria monocytogenes i u jednom protozoe Neospora caninum. U 93,3% ispitanih
uzoraka nije dokazan nijedan od pravilnikom predviđenih infektivnih uzročnika
pobačaja. Indirektnim, serološkim metodama dijagnostike utvrđena je visoka
seroprevalencija na Neospora caninum (29,2%), dok su hlamidioza i kju-groznica
potvrđene redom u 2,5% i 10,2% ispitanih uzoraka. Serološki odgovor na virus
goveđe virusne dijareje i virus infektivnog bovinog rinotraheitisa/pustuloznog
vulvovaginitisa ustanovljen je redom kod 5,3% odnosno 4,2% ispitanih jedinki sa
individualnih gazdinstava, dok su kod životinja sa komercijalne farme usled
primene vakcinacije dobijeni procenti značajno viši (43,5% i 87%)., Abortion in cattle is defined as the termination of pregnancy and expulsion of an
immature fetus from the uterus between the 42nd and 260th day of pregnancy.
Abortions can be caused by infectious and non-infectious causative agents. Infectious
agents, besides abortion, can cause additional health disorders, leading to significant
direct and indirect economic losses. Some of them (Brucella spp, Coxiella burnetii,
Listeria monocytogenes) pose a risk to human health as well. Accordingly, laboratory
diagnostics of infectious abortions in cattle is a part of the mandatory surveillance of
cattle health in Serbia. It is dictated on an annual basis through the Legal regulation
on the Program of the Animal Health Protection Measures. In the period from 2019
to 2022, 118 sera and 105 samples of pathological material from aborted fetuses were
collected on the territory of the Belgrade epizootiological area and analyzed at the
Institute of Veterinary Medicine of Serbia. Of these, 23 serа and 17 aborted fetuses
originated from commercial cattle farms, while 95 serа and 88 aborted fetuses were
sampled on farms characterized by extensive production. By methods of direct
detection in the pathological material from fetuses bovine viral diarrhea virus was
detected in two, Listeria monocytogenes in four, and Neospora caninum in one
sample. In 93.3% of analyzed samples, none of the tested pathogens was detected.
Indirect, serological diagnostic methods revealed a high seroprevalence of Neospora
caninum (29.2%), while Chlamydiosis and Q fever were confirmed in 2.5% and
10.2% of the tested samples, respectively. A serological response to the bovine viral
diarrhoea virus and infectious bovine rhinotracheitis/pustular vulvovaginitis virus
were found in 5.3% and 4.2% of the samples from extensive production, respectively,
while due to the vaccination, percentages were significantly higher in analyzed cattle
from intensive production (43.5% and 87% respectively).",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani)",
title = "Rezultati laboratorijskih ispitivanja kod pobačaja goveda na teritoriji Beogradskog epizootiološkog područja u periodu od 2019. do 2022. godine, Results of laboratory examinations of abortions in cattle on the Belgrade epizootiological area in the period from 2019 - 2022",
pages = "97-96"
}

Maksimović Zorić, J., Veljović, L., Glišić, D., Vojinovic, D., Zurovac Sapundžić, Z., Ninković, M., Zdravković, N., Radanović, O., Jezdimirović, N., Stanojević, S.,& Žutić, J.. (2023). Rezultati laboratorijskih ispitivanja kod pobačaja goveda na teritoriji Beogradskog epizootiološkog područja u periodu od 2019. do 2022. godine. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani)
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 96-97.

Maksimović Zorić J, Veljović L, Glišić D, Vojinovic D, Zurovac Sapundžić Z, Ninković M, Zdravković N, Radanović O, Jezdimirović N, Stanojević S, Žutić J. Rezultati laboratorijskih ispitivanja kod pobačaja goveda na teritoriji Beogradskog epizootiološkog područja u periodu od 2019. do 2022. godine. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani). 2023;:96-97..

Maksimović Zorić, Jelena, Veljović, Ljubiša, Glišić, Dimitrije, Vojinovic, Dragica, Zurovac Sapundžić, Zorana, Ninković, Milan, Zdravković, Nemanja, Radanović, Oliver, Jezdimirović, Nemanja, Stanojević, Slobodan, Žutić, Jadranka, "Rezultati laboratorijskih ispitivanja kod pobačaja goveda na teritoriji Beogradskog epizootiološkog područja u periodu od 2019. do 2022. godine" in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani) (2023):96-97.

TY  - CONF
AU  - Milićević, Vesna
AU  - Glišić, Dimitrije
AU  - Veljović, Ljubiša
AU  - Savić, Božidar
AU  - Kureljušić, Branislav
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/539
AB  - Virus reproduktivnog i respiratornog sindroma svinja (PRRSV) je prvi put u
Srbiji otkriven 2001. godine. PRRSV pripada familiji Arteriviridae i
razvrstava se u dva genotipa, genotip PRRSV-1 i PRRSV-2. Dok PRRSV-1
uglavnom uzrokuje respiratorne simptome, PRRSV-2 dovodi do
reproduktivnih poremećaja. Ekonomske štete koje su posledica PRRS-a su
značajne, te se danas PRRS kontroliše vakcinacijom sa više ili manje uspeha.
Pošto je virus, a naročito tip 1, izuzetno varijabilan, sa visokom stopom
mutacija i rekombinacija, potrebno je stalno praćenje sojeva, odnosno njihovo
menjanje u odnosu na dijagnostičke protokole i vakcinu. Dijagnostika PRRS-a
je upravo usled stalnih promena genoma veoma kompleksna, pa se svi sojevi
istog genotipa ne mogu detektovati jednim PCR protokolom. Genetske razlike
između PRRSV-1 i PRRSV-2 su tek značajne. Nadalje, usled primene
vakcinacije, serološka dijagnostika ima ograničenu primenu. Do 2022.
godine, u Srbiji je cirkulisao PRRS-1. U ovom radu je opisan prvi slučaj
dijagnostike i kontrole PRRSV-2 na farmi na kojoj je PRRSV-1 kontrolisan
vakcinacijom.
AB  - Porcine reproductive and respiratory syndrome virus (PRRSV) was first discovered
in Serbia in 2001. PRRSV belongs to the family Arteriviridae and is further classified
into two genotypes, genotype PRRSV-1 and PRRSV-2. While PRRSV-1 mainly
causes respiratory symptoms, PRRSV-2 leads to reproductive disorders. The
economic losses caused by PRRS are significant, and nowadays PRRS is controlled
by vaccination with more or less success. Since the virus, especially type 1, is
extremely variable, with high mutation and recombination rates, it is necessary to
constantly monitor the strains, and their changes in terms of diagnostic protocols and
vaccines. The diagnosis of PRRS is very complex precisely because of the constant
changes in the genome, so all strains of the same genotype cannot be detected by a
single PCR protocol. Genetic differences between PRRSV-1 and PRRSV-2 are even
more significant. Furthermore, due to the use of vaccination, serological diagnostics
has a limited application. Until 2022, PRRS-1 circulated in Serbia. This paper
describes the first case of diagnosis and control of PRRSV-2 on a farm where PRRSV-
1 was controlled by vaccination.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani)
T1  - Dijagnostika i karakteristike simultane PRRSV-1 I PRRSV-2 infekcije
T1  - Diagnosis and characteristics of simultaneous PRRSV-1 and PRRSV-2 infection
EP  - 83
SP  - 82
ER  - 

@conference{
author = "Milićević, Vesna and Glišić, Dimitrije and Veljović, Ljubiša and Savić, Božidar and Kureljušić, Branislav",
year = "2023",
abstract = "Virus reproduktivnog i respiratornog sindroma svinja (PRRSV) je prvi put u
Srbiji otkriven 2001. godine. PRRSV pripada familiji Arteriviridae i
razvrstava se u dva genotipa, genotip PRRSV-1 i PRRSV-2. Dok PRRSV-1
uglavnom uzrokuje respiratorne simptome, PRRSV-2 dovodi do
reproduktivnih poremećaja. Ekonomske štete koje su posledica PRRS-a su
značajne, te se danas PRRS kontroliše vakcinacijom sa više ili manje uspeha.
Pošto je virus, a naročito tip 1, izuzetno varijabilan, sa visokom stopom
mutacija i rekombinacija, potrebno je stalno praćenje sojeva, odnosno njihovo
menjanje u odnosu na dijagnostičke protokole i vakcinu. Dijagnostika PRRS-a
je upravo usled stalnih promena genoma veoma kompleksna, pa se svi sojevi
istog genotipa ne mogu detektovati jednim PCR protokolom. Genetske razlike
između PRRSV-1 i PRRSV-2 su tek značajne. Nadalje, usled primene
vakcinacije, serološka dijagnostika ima ograničenu primenu. Do 2022.
godine, u Srbiji je cirkulisao PRRS-1. U ovom radu je opisan prvi slučaj
dijagnostike i kontrole PRRSV-2 na farmi na kojoj je PRRSV-1 kontrolisan
vakcinacijom., Porcine reproductive and respiratory syndrome virus (PRRSV) was first discovered
in Serbia in 2001. PRRSV belongs to the family Arteriviridae and is further classified
into two genotypes, genotype PRRSV-1 and PRRSV-2. While PRRSV-1 mainly
causes respiratory symptoms, PRRSV-2 leads to reproductive disorders. The
economic losses caused by PRRS are significant, and nowadays PRRS is controlled
by vaccination with more or less success. Since the virus, especially type 1, is
extremely variable, with high mutation and recombination rates, it is necessary to
constantly monitor the strains, and their changes in terms of diagnostic protocols and
vaccines. The diagnosis of PRRS is very complex precisely because of the constant
changes in the genome, so all strains of the same genotype cannot be detected by a
single PCR protocol. Genetic differences between PRRSV-1 and PRRSV-2 are even
more significant. Furthermore, due to the use of vaccination, serological diagnostics
has a limited application. Until 2022, PRRS-1 circulated in Serbia. This paper
describes the first case of diagnosis and control of PRRSV-2 on a farm where PRRSV-
1 was controlled by vaccination.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani)",
title = "Dijagnostika i karakteristike simultane PRRSV-1 I PRRSV-2 infekcije, Diagnosis and characteristics of simultaneous PRRSV-1 and PRRSV-2 infection",
pages = "83-82"
}

Milićević, V., Glišić, D., Veljović, L., Savić, B.,& Kureljušić, B.. (2023). Dijagnostika i karakteristike simultane PRRSV-1 I PRRSV-2 infekcije. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani)
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 82-83.

Milićević V, Glišić D, Veljović L, Savić B, Kureljušić B. Dijagnostika i karakteristike simultane PRRSV-1 I PRRSV-2 infekcije. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani). 2023;:82-83..

Milićević, Vesna, Glišić, Dimitrije, Veljović, Ljubiša, Savić, Božidar, Kureljušić, Branislav, "Dijagnostika i karakteristike simultane PRRSV-1 I PRRSV-2 infekcije" in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani) (2023):82-83.

TY  - JOUR
AU  - Maletić, Jelena
AU  - Spalević, Ljiljana
AU  - Kureljušić, Branislav
AU  - Veljović, Ljubiša
AU  - Maksimović Zorić, Jelena
AU  - Maletić, Milan
AU  - Milićević, Vesna
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/494
AB  - Fowl adenovirus infections have a significant economic impact, especially in the production of broilers. It is considered the leading cause of three syndromes: adenoviral gizzard erosions and ulcerations, inclusion body hepatitis, and hepatitis-hydropericardium syndrome. A critical feature of this virus is its immunosuppressive effect, via suppressing humoral and cellular immunity.In this study, we examined the humoral immune response after administration of the Newcastle disease vaccine in broiler flocks with previously confirmed seroconversion against Fowl adenovirus. The study was conducted on 5 farms. A total of 220 chickens, five weeks of age, showing no clinical signs of the disease, were included in this study. The control group consisted of 20 chickens from a negative farm. Chickens were vaccinated with commercially available live NDV vaccines between 11 and 13 days of life. ELISA determined the presence of specific antibodies against FAdV in a total of 130/200 (65%) blood sera. Depending on the farm, seroprevalence ranged from 30-100%. The presence of specific antibodies against NDV was determined three weeks after vaccination using the hemagglutination inhibition assay. A positive hemagglutination inhibition (HI) titer (>= 16) was found in 41/200 (20.5%) sera, which was significantly less compared to the control farm, where a positive HI titer was found in 20/20 (100%) sera.The results of our study indicate the immunosuppressive effect of FAdV in subclinically infected birds and highlight the need for its diagnosis, prevention, and control.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Fowl adenovirus infection - potential cause of a suppressed humoral immune response of broilers to newcastle disease vaccination
EP  - 142
IS  - 1
SP  - 133
VL  - 73
DO  - 10.2478/acve-2023-0010
UR  - conv_604
ER  - 

@article{
author = "Maletić, Jelena and Spalević, Ljiljana and Kureljušić, Branislav and Veljović, Ljubiša and Maksimović Zorić, Jelena and Maletić, Milan and Milićević, Vesna",
year = "2023",
abstract = "Fowl adenovirus infections have a significant economic impact, especially in the production of broilers. It is considered the leading cause of three syndromes: adenoviral gizzard erosions and ulcerations, inclusion body hepatitis, and hepatitis-hydropericardium syndrome. A critical feature of this virus is its immunosuppressive effect, via suppressing humoral and cellular immunity.In this study, we examined the humoral immune response after administration of the Newcastle disease vaccine in broiler flocks with previously confirmed seroconversion against Fowl adenovirus. The study was conducted on 5 farms. A total of 220 chickens, five weeks of age, showing no clinical signs of the disease, were included in this study. The control group consisted of 20 chickens from a negative farm. Chickens were vaccinated with commercially available live NDV vaccines between 11 and 13 days of life. ELISA determined the presence of specific antibodies against FAdV in a total of 130/200 (65%) blood sera. Depending on the farm, seroprevalence ranged from 30-100%. The presence of specific antibodies against NDV was determined three weeks after vaccination using the hemagglutination inhibition assay. A positive hemagglutination inhibition (HI) titer (>= 16) was found in 41/200 (20.5%) sera, which was significantly less compared to the control farm, where a positive HI titer was found in 20/20 (100%) sera.The results of our study indicate the immunosuppressive effect of FAdV in subclinically infected birds and highlight the need for its diagnosis, prevention, and control.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Fowl adenovirus infection - potential cause of a suppressed humoral immune response of broilers to newcastle disease vaccination",
pages = "142-133",
number = "1",
volume = "73",
doi = "10.2478/acve-2023-0010",
url = "conv_604"
}

Maletić, J., Spalević, L., Kureljušić, B., Veljović, L., Maksimović Zorić, J., Maletić, M.,& Milićević, V.. (2023). Fowl adenovirus infection - potential cause of a suppressed humoral immune response of broilers to newcastle disease vaccination. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 73(1), 133-142.
https://doi.org/10.2478/acve-2023-0010
conv_604

Maletić J, Spalević L, Kureljušić B, Veljović L, Maksimović Zorić J, Maletić M, Milićević V. Fowl adenovirus infection - potential cause of a suppressed humoral immune response of broilers to newcastle disease vaccination. in Acta Veterinaria-Beograd. 2023;73(1):133-142.
doi:10.2478/acve-2023-0010
conv_604 .

Maletić, Jelena, Spalević, Ljiljana, Kureljušić, Branislav, Veljović, Ljubiša, Maksimović Zorić, Jelena, Maletić, Milan, Milićević, Vesna, "Fowl adenovirus infection - potential cause of a suppressed humoral immune response of broilers to newcastle disease vaccination" in Acta Veterinaria-Beograd, 73, no. 1 (2023):133-142,
https://doi.org/10.2478/acve-2023-0010 .,
conv_604 .

TY  - CONF
AU  - Maksimović Zorić, Jelena
AU  - Veljović, Ljubiša
AU  - Glišić, Dimitrije
AU  - Kureljušić, Branislav
AU  - Jezdimirović, Nemanja
AU  - Radosavljevic, Vladimir
AU  - Milićević, Vesna
PY  - 2022
UR  - https://reponivs.nivs.rs/handle/123456789/582
AB  - Инфлуенца свиња је инфективно респираторно обољење свиња проузроковано
вирусима инфлуенце типа А. С обзиром на њихову глобалну распрострањеност
у популацијама свиња, негативан утицај на економичност производње и
зоонотски потенцијал који поседују, ови вируси представљају значајан проблем
у ветеринарској, а повремено и у хуманој медицини. У циљу ефикасније
контроле и превенције ове инфекције на фармама свиња и одређивања
пандемијског потенцијала циркулишућих вируса анализиране су молекуларне
карактеристике протеина пет вируса инфлуенце А свиња изолованих у периоду
од 2016. до 2018. године на комерцијалним фармама са територије северне и
централне Србије. Испитане карактеристике су од значаја за репликацију у
ћелијама свиња и људи, протективни ефекат вакцина и резистенцију на
антивирусне лекове који се примењују у хуманој медицини.
Анализом грађе рецепторског места на хемаглутинину установљено је да сви
вируси поседују афинитет ка α2-6 типу рецептора (190D/225D и 190D/225E)
који доминирају у респираторном тракту свиња али и људи. Молекуларни
маркери детектовани у саставу полимеразе Б2 (627Е, 701Н, 590С/591Р, 251К),
полимеразе Б1 (3В, 622Г и 678Н), полимеразе А и способност синтезе
акцесорних протеина (ПБ1-Ф2, ПБ1-Н40) указују на олакшано умножавање
ових вируса у ћелијским линијама пореклом од сисара и код лабораторијских
животиња. Код свих анализираних вируса утврђено је између 2 и 7
аминокиселинских измена у саставу антигенских места хемаглутинина (Sa, Sb,
Ca1, Ca2 и Cb) у односу на грађу антигенских места код сојева у саставу
најчешће коришћених комерцијалних вакцина. Аминокиселине на позицијама
119, 136, 150, 199, 223, 247, 275 и 295 у ланцу неураминидазе наших вируса
указују на осетљивост на лекове из групе инхибитора неураминидаза, док
присуство аспарагина на позицији 31 у аминокислеинском ланцу матрикс 2
протеина обезбеђује испитаним вирусима резистенцију на дејство блокатора М2
јонских канала. Ови налази су од посебног значаја у случају "прескакања"
баријере врсте, те последичне инфекције и развоја клиничке слике код људи.
AB  - Swine influenza is a respiratory infectious disease of pigs caused by influenza A
viruses. Given their global distribution in pig populations, the negative economic
impact on pig production and their zoonotic potential, these viruses present a
significant problem in veterinary and occasionally in human medicine. In order to
more effectively control and prevent this infection in pig farms and determine the
pandemic potential of circulating viruses, molecular characteristics of proteins of five
swine influenza A viruses isolated in period from 2016th to 2018th in commercial farms
located in North and Central Serbia have been analysed. These molecular
characteristics are important for replication in pig and human cells, protective effect
of vaccines and resistance to antiviral drugs.
By analysis of the structure of receptor-binding site of hemagglutinin, it was shown
that all viruses possess an affinity for the α2-6 type of receptor (190D/225D and
190D/225E) dominant in respiratory tract of pigs and humans. Molecular markers
detected within polymerase B2 (627E, 701N, 590S/591R, 251K), polymerase B1 (3B,
622G and 678N), polymerase A and synthesis of accessory proteins (PB1-F2, PB1-
N40) point to facilitated replication in mammalian cell lines and in laboratory animals.
Within antigenic sites of hemagglutinin (Sa, Sb, Ca1, Ca2 and Cb) it was found 2 to
7 amino acid changes in comparison to the structure of antigenic sites of strains within
commonly used commercial vaccines. Amino acids at positions 119, 136, 150, 199,
223, 247, 275 and 295 within neuraminidase indicate the sensitivity to the drugs from
the group of neuraminidase inhibitors, while presence of asparagine at position 31 in
the matrix 2 protein amino acid chain provides resistance to the M2 ion channel
blockers. These findings are of particular importance in the case of the crossing
species barrier and the consequent infection and development of the clinical signs in
humans.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXIV Simpozijum epizootiologa i epidemiologa (XXIV Epizootiološki dani)
T1  - Molekularne karakteristike proteina virusa influence tipa A svinja u Srbiji
T1  - Molecular characteristics of proteins of Serbian swine influenza A virus
EP  - 103
SP  - 102
ER  - 

@conference{
author = "Maksimović Zorić, Jelena and Veljović, Ljubiša and Glišić, Dimitrije and Kureljušić, Branislav and Jezdimirović, Nemanja and Radosavljevic, Vladimir and Milićević, Vesna",
year = "2022",
abstract = "Инфлуенца свиња је инфективно респираторно обољење свиња проузроковано
вирусима инфлуенце типа А. С обзиром на њихову глобалну распрострањеност
у популацијама свиња, негативан утицај на економичност производње и
зоонотски потенцијал који поседују, ови вируси представљају значајан проблем
у ветеринарској, а повремено и у хуманој медицини. У циљу ефикасније
контроле и превенције ове инфекције на фармама свиња и одређивања
пандемијског потенцијала циркулишућих вируса анализиране су молекуларне
карактеристике протеина пет вируса инфлуенце А свиња изолованих у периоду
од 2016. до 2018. године на комерцијалним фармама са територије северне и
централне Србије. Испитане карактеристике су од значаја за репликацију у
ћелијама свиња и људи, протективни ефекат вакцина и резистенцију на
антивирусне лекове који се примењују у хуманој медицини.
Анализом грађе рецепторског места на хемаглутинину установљено је да сви
вируси поседују афинитет ка α2-6 типу рецептора (190D/225D и 190D/225E)
који доминирају у респираторном тракту свиња али и људи. Молекуларни
маркери детектовани у саставу полимеразе Б2 (627Е, 701Н, 590С/591Р, 251К),
полимеразе Б1 (3В, 622Г и 678Н), полимеразе А и способност синтезе
акцесорних протеина (ПБ1-Ф2, ПБ1-Н40) указују на олакшано умножавање
ових вируса у ћелијским линијама пореклом од сисара и код лабораторијских
животиња. Код свих анализираних вируса утврђено је између 2 и 7
аминокиселинских измена у саставу антигенских места хемаглутинина (Sa, Sb,
Ca1, Ca2 и Cb) у односу на грађу антигенских места код сојева у саставу
најчешће коришћених комерцијалних вакцина. Аминокиселине на позицијама
119, 136, 150, 199, 223, 247, 275 и 295 у ланцу неураминидазе наших вируса
указују на осетљивост на лекове из групе инхибитора неураминидаза, док
присуство аспарагина на позицији 31 у аминокислеинском ланцу матрикс 2
протеина обезбеђује испитаним вирусима резистенцију на дејство блокатора М2
јонских канала. Ови налази су од посебног значаја у случају "прескакања"
баријере врсте, те последичне инфекције и развоја клиничке слике код људи., Swine influenza is a respiratory infectious disease of pigs caused by influenza A
viruses. Given their global distribution in pig populations, the negative economic
impact on pig production and their zoonotic potential, these viruses present a
significant problem in veterinary and occasionally in human medicine. In order to
more effectively control and prevent this infection in pig farms and determine the
pandemic potential of circulating viruses, molecular characteristics of proteins of five
swine influenza A viruses isolated in period from 2016th to 2018th in commercial farms
located in North and Central Serbia have been analysed. These molecular
characteristics are important for replication in pig and human cells, protective effect
of vaccines and resistance to antiviral drugs.
By analysis of the structure of receptor-binding site of hemagglutinin, it was shown
that all viruses possess an affinity for the α2-6 type of receptor (190D/225D and
190D/225E) dominant in respiratory tract of pigs and humans. Molecular markers
detected within polymerase B2 (627E, 701N, 590S/591R, 251K), polymerase B1 (3B,
622G and 678N), polymerase A and synthesis of accessory proteins (PB1-F2, PB1-
N40) point to facilitated replication in mammalian cell lines and in laboratory animals.
Within antigenic sites of hemagglutinin (Sa, Sb, Ca1, Ca2 and Cb) it was found 2 to
7 amino acid changes in comparison to the structure of antigenic sites of strains within
commonly used commercial vaccines. Amino acids at positions 119, 136, 150, 199,
223, 247, 275 and 295 within neuraminidase indicate the sensitivity to the drugs from
the group of neuraminidase inhibitors, while presence of asparagine at position 31 in
the matrix 2 protein amino acid chain provides resistance to the M2 ion channel
blockers. These findings are of particular importance in the case of the crossing
species barrier and the consequent infection and development of the clinical signs in
humans.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXIV Simpozijum epizootiologa i epidemiologa (XXIV Epizootiološki dani)",
title = "Molekularne karakteristike proteina virusa influence tipa A svinja u Srbiji, Molecular characteristics of proteins of Serbian swine influenza A virus",
pages = "103-102"
}

Maksimović Zorić, J., Veljović, L., Glišić, D., Kureljušić, B., Jezdimirović, N., Radosavljevic, V.,& Milićević, V.. (2022). Molekularne karakteristike proteina virusa influence tipa A svinja u Srbiji. in XXIV Simpozijum epizootiologa i epidemiologa (XXIV Epizootiološki dani)
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 102-103.

Maksimović Zorić J, Veljović L, Glišić D, Kureljušić B, Jezdimirović N, Radosavljevic V, Milićević V. Molekularne karakteristike proteina virusa influence tipa A svinja u Srbiji. in XXIV Simpozijum epizootiologa i epidemiologa (XXIV Epizootiološki dani). 2022;:102-103..

Maksimović Zorić, Jelena, Veljović, Ljubiša, Glišić, Dimitrije, Kureljušić, Branislav, Jezdimirović, Nemanja, Radosavljevic, Vladimir, Milićević, Vesna, "Molekularne karakteristike proteina virusa influence tipa A svinja u Srbiji" in XXIV Simpozijum epizootiologa i epidemiologa (XXIV Epizootiološki dani) (2022):102-103.

TY  - CONF
AU  - Maletić, Jelena
AU  - Kureljušić, Branislav
AU  - Spalević, Ljiljana
AU  - Vučićević, Ivana
AU  - Veljović, Ljubiša
AU  - Milovanović, Bojan
AU  - Milićević, Vesna
PY  - 2022
UR  - https://reponivs.nivs.rs/handle/123456789/924
AB  - Marek’s disease virus and avian leucosis virus are causes of contagious, immunosuppressive, and oncogenic diseases. Many reports have described single virus infection, but detailed description of co-infection with Marek’s disease virus and avian leucosis virus has been provided in a few previous reports. The aim of this study was to determine the cause of health disorders in a flock of the Banat Naked Neck hens at the age of 11 weeks. Clinical examination of the flock showed signs of cachexia, apathy, dropped wings, nervous symptoms in the form of bilateral leg paresis, sporadic diarrhoea, and the mortality reaching 20%. Post-mortem examinations showed the following changes: chronic pseudomembranous typhlitis, intestinal ascaridiasis, splenomegaly, granulomatous pneumonia, multiple yellowish caseous foci in the liver, and white tumour masses in the heart. Histopathological examination of the spleen, proventriculus and heart tissue revealed polymorphic infiltrates, while granulomatous inflammation was observed in the lung and liver. Gomori methenamine silver stain method revealed septate and arborized hyphae resembling the Aspergillus spp. fungi in the liver and lung granulomas. Bacteriological examination of faeces revealed the presence of Escherichia coli, and parasitological examination of the small intestine determined the presence of oocyst of Eimeria acervulina and Eimeria tenella in the caecum. The genomes of Marek's disease virus and avian leucosis virus were determined by polymerase chain reaction in tissue samples. In this case, specific pathological lesions, as well as molecular finding confirmed co-infection with Marek’s disease virus and avian leucosis virus.
PB  - Zagreb : Hrvatski veterinarski institut, Centar za peradarstvo
C3  - XIV Simpozij pereradarski dani 2022. s međunarodnim sudelovanjem/XIV Symposium poultry days 2022 With International Participation, Hrvatska, Poreć, 11.-14. svibnja 2022.
T1  - Co-infection with Marek’s disease virus and avian leukosis virus in the Banat Naked Neck hens
T1  - Koinfekcija virusom Marekove bolesti i virusom ptičje leukoze kod kokoši pasmine banatski gološijan
EP  - 217
SP  - 210
ER  - 

@conference{
author = "Maletić, Jelena and Kureljušić, Branislav and Spalević, Ljiljana and Vučićević, Ivana and Veljović, Ljubiša and Milovanović, Bojan and Milićević, Vesna",
year = "2022",
abstract = "Marek’s disease virus and avian leucosis virus are causes of contagious, immunosuppressive, and oncogenic diseases. Many reports have described single virus infection, but detailed description of co-infection with Marek’s disease virus and avian leucosis virus has been provided in a few previous reports. The aim of this study was to determine the cause of health disorders in a flock of the Banat Naked Neck hens at the age of 11 weeks. Clinical examination of the flock showed signs of cachexia, apathy, dropped wings, nervous symptoms in the form of bilateral leg paresis, sporadic diarrhoea, and the mortality reaching 20%. Post-mortem examinations showed the following changes: chronic pseudomembranous typhlitis, intestinal ascaridiasis, splenomegaly, granulomatous pneumonia, multiple yellowish caseous foci in the liver, and white tumour masses in the heart. Histopathological examination of the spleen, proventriculus and heart tissue revealed polymorphic infiltrates, while granulomatous inflammation was observed in the lung and liver. Gomori methenamine silver stain method revealed septate and arborized hyphae resembling the Aspergillus spp. fungi in the liver and lung granulomas. Bacteriological examination of faeces revealed the presence of Escherichia coli, and parasitological examination of the small intestine determined the presence of oocyst of Eimeria acervulina and Eimeria tenella in the caecum. The genomes of Marek's disease virus and avian leucosis virus were determined by polymerase chain reaction in tissue samples. In this case, specific pathological lesions, as well as molecular finding confirmed co-infection with Marek’s disease virus and avian leucosis virus.",
publisher = "Zagreb : Hrvatski veterinarski institut, Centar za peradarstvo",
journal = "XIV Simpozij pereradarski dani 2022. s međunarodnim sudelovanjem/XIV Symposium poultry days 2022 With International Participation, Hrvatska, Poreć, 11.-14. svibnja 2022.",
title = "Co-infection with Marek’s disease virus and avian leukosis virus in the Banat Naked Neck hens, Koinfekcija virusom Marekove bolesti i virusom ptičje leukoze kod kokoši pasmine banatski gološijan",
pages = "217-210"
}

Maletić, J., Kureljušić, B., Spalević, L., Vučićević, I., Veljović, L., Milovanović, B.,& Milićević, V.. (2022). Co-infection with Marek’s disease virus and avian leukosis virus in the Banat Naked Neck hens. in XIV Simpozij pereradarski dani 2022. s međunarodnim sudelovanjem/XIV Symposium poultry days 2022 With International Participation, Hrvatska, Poreć, 11.-14. svibnja 2022.
Zagreb : Hrvatski veterinarski institut, Centar za peradarstvo., 210-217.

Maletić J, Kureljušić B, Spalević L, Vučićević I, Veljović L, Milovanović B, Milićević V. Co-infection with Marek’s disease virus and avian leukosis virus in the Banat Naked Neck hens. in XIV Simpozij pereradarski dani 2022. s međunarodnim sudelovanjem/XIV Symposium poultry days 2022 With International Participation, Hrvatska, Poreć, 11.-14. svibnja 2022.. 2022;:210-217..

Maletić, Jelena, Kureljušić, Branislav, Spalević, Ljiljana, Vučićević, Ivana, Veljović, Ljubiša, Milovanović, Bojan, Milićević, Vesna, "Co-infection with Marek’s disease virus and avian leukosis virus in the Banat Naked Neck hens" in XIV Simpozij pereradarski dani 2022. s međunarodnim sudelovanjem/XIV Symposium poultry days 2022 With International Participation, Hrvatska, Poreć, 11.-14. svibnja 2022. (2022):210-217.

TY  - CONF
AU  - Radosavljevic, Vladimir
AU  - Glišić, Dimitrije
AU  - Radanović, Oliver
AU  - Zdravković, Nemanja
AU  - Maksimović Zorić, Jelena
AU  - Veljović, Ljubiša
AU  - Milićević, Vesna
PY  - 2022
UR  - https://reponivs.nivs.rs/handle/123456789/586
AB  - У Србији је сектор слатководне аквакултуре заступљен кроз широк спектар
производних метода у распону од екстензивних до технолошки високоинтензивних метода. Током последњих деценија дијагностиковано је више
болести узгајаних и дивљих риба, које су створиле велике проблеме у
аквакултури, и стога су предмет надзора и мониторинга у многим земљама.
Данас, болести риба могу у врло кратком временском периоду ући на нову
територију, а увоз украсних риба посебно олакшава ширење болести. Због све
веће важности проблема са болестима у аквакултури и утицаја које болести могу
имати на запате риба у природи и аквакултури, праћење и надзор болести риба
је од великог значаја за даљи раст и одрживост аквакултуре. Контрола здравља
риба у аквакултури Србије се врши на основу националног законодавства.
Поред тога, спроводе се принципи постављени у правилима ОИЕ и уредбама и
директивама Европске уније, у погледу захтева за здравље риба у аквакултури.
Међутим, повећање производње, диверзификација и интензивна трговина
рибом и производима риба стварају додатни притисак, повезан са избијањем
болести, који кочи раст аквакултуре. Поред болести наведених у законодавству
ЕУ, Национална референтна лабораторија за болести риба рутински тестира
пријемчиве врсте риба и на друге болести које су од економског значаја,
обзиром да велики број различитих поремећаја здравственог стања, како
инфективне тако и неинфективне природе, може проузроковати значајне
губитке и ограничити развој аквакултуре. У раду је дат преглед аквакултуре,
статуса законодавства и најважнијих болести риба у Србији у 2021. години.
AB  - In Serbia, the freshwater aquaculture sector is represented through a wide range of
production methods ranging from the most extensive ones to the high technological
intensive methods. Over the last decades, several diseases in fish have been diagnosed
in farmed and feral populations, creating large problems in the fish farming industry
and thus being the subject of surveillance and monitoring programmes in many
countries. Nowadays, fish diseases can enter a new territory within a very short period
of time, and imports of ornamental fish make the spread of fish diseases especially
easy. Due to the increasing importance of disease problems in aquaculture and the
impact disease may have on both feral and farmed fish, monitoring and surveillance
on fish diseases is considered to be of high importance for further growth and
sustainability of aquaculture. Fish health control in Serbian aquaculture is based on
national legislation for surveillance and disease control in aquatic animals and
regulations listing notifiable diseases of concern. In addition, the principles laid down
in the WHO rules and European Union regulations and directives, as regards animal
health requirements for aquaculture animals and products thereof are implemented.
However, increase of production, diversification, and the intensive trade of fish and
fish products create an extra pressure, related to disease outbreaks, that hampers
aquaculture growth. Apart from the diseases listed under EU legislation, the National
reference laboratory for fish diseases also routinely tests for other diseases which are
of economic importance in Serbia, since a large number of various health problems,
both infectious and non-infectious, may cause significant losses and limit aquaculture.
The paper provides an overview of aquaculture, legislation status and the most
important fish diseases in Serbia in 2021.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXIV Simpozijum epizootiologa i epidemiologa (XXIV Epizootiološki dani)
T1  - Аквакултура и болести риба у 2021. години
T1  - Akvakultura i bolesti riba u 2021. godini
EP  - 43
SP  - 42
ER  - 

@conference{
author = "Radosavljevic, Vladimir and Glišić, Dimitrije and Radanović, Oliver and Zdravković, Nemanja and Maksimović Zorić, Jelena and Veljović, Ljubiša and Milićević, Vesna",
year = "2022",
abstract = "У Србији је сектор слатководне аквакултуре заступљен кроз широк спектар
производних метода у распону од екстензивних до технолошки високоинтензивних метода. Током последњих деценија дијагностиковано је више
болести узгајаних и дивљих риба, које су створиле велике проблеме у
аквакултури, и стога су предмет надзора и мониторинга у многим земљама.
Данас, болести риба могу у врло кратком временском периоду ући на нову
територију, а увоз украсних риба посебно олакшава ширење болести. Због све
веће важности проблема са болестима у аквакултури и утицаја које болести могу
имати на запате риба у природи и аквакултури, праћење и надзор болести риба
је од великог значаја за даљи раст и одрживост аквакултуре. Контрола здравља
риба у аквакултури Србије се врши на основу националног законодавства.
Поред тога, спроводе се принципи постављени у правилима ОИЕ и уредбама и
директивама Европске уније, у погледу захтева за здравље риба у аквакултури.
Међутим, повећање производње, диверзификација и интензивна трговина
рибом и производима риба стварају додатни притисак, повезан са избијањем
болести, који кочи раст аквакултуре. Поред болести наведених у законодавству
ЕУ, Национална референтна лабораторија за болести риба рутински тестира
пријемчиве врсте риба и на друге болести које су од економског значаја,
обзиром да велики број различитих поремећаја здравственог стања, како
инфективне тако и неинфективне природе, може проузроковати значајне
губитке и ограничити развој аквакултуре. У раду је дат преглед аквакултуре,
статуса законодавства и најважнијих болести риба у Србији у 2021. години., In Serbia, the freshwater aquaculture sector is represented through a wide range of
production methods ranging from the most extensive ones to the high technological
intensive methods. Over the last decades, several diseases in fish have been diagnosed
in farmed and feral populations, creating large problems in the fish farming industry
and thus being the subject of surveillance and monitoring programmes in many
countries. Nowadays, fish diseases can enter a new territory within a very short period
of time, and imports of ornamental fish make the spread of fish diseases especially
easy. Due to the increasing importance of disease problems in aquaculture and the
impact disease may have on both feral and farmed fish, monitoring and surveillance
on fish diseases is considered to be of high importance for further growth and
sustainability of aquaculture. Fish health control in Serbian aquaculture is based on
national legislation for surveillance and disease control in aquatic animals and
regulations listing notifiable diseases of concern. In addition, the principles laid down
in the WHO rules and European Union regulations and directives, as regards animal
health requirements for aquaculture animals and products thereof are implemented.
However, increase of production, diversification, and the intensive trade of fish and
fish products create an extra pressure, related to disease outbreaks, that hampers
aquaculture growth. Apart from the diseases listed under EU legislation, the National
reference laboratory for fish diseases also routinely tests for other diseases which are
of economic importance in Serbia, since a large number of various health problems,
both infectious and non-infectious, may cause significant losses and limit aquaculture.
The paper provides an overview of aquaculture, legislation status and the most
important fish diseases in Serbia in 2021.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXIV Simpozijum epizootiologa i epidemiologa (XXIV Epizootiološki dani)",
title = "Аквакултура и болести риба у 2021. години, Akvakultura i bolesti riba u 2021. godini",
pages = "43-42"
}

Radosavljevic, V., Glišić, D., Radanović, O., Zdravković, N., Maksimović Zorić, J., Veljović, L.,& Milićević, V.. (2022). Аквакултура и болести риба у 2021. години. in XXIV Simpozijum epizootiologa i epidemiologa (XXIV Epizootiološki dani)
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 42-43.

Radosavljevic V, Glišić D, Radanović O, Zdravković N, Maksimović Zorić J, Veljović L, Milićević V. Аквакултура и болести риба у 2021. години. in XXIV Simpozijum epizootiologa i epidemiologa (XXIV Epizootiološki dani). 2022;:42-43..

Radosavljevic, Vladimir, Glišić, Dimitrije, Radanović, Oliver, Zdravković, Nemanja, Maksimović Zorić, Jelena, Veljović, Ljubiša, Milićević, Vesna, "Аквакултура и болести риба у 2021. години" in XXIV Simpozijum epizootiologa i epidemiologa (XXIV Epizootiološki dani) (2022):42-43.

TY  - CONF
AU  - Radosavljevic, Vladimir
AU  - Radanović, Oliver
AU  - Jezdimirović, Nemanja
AU  - Glišić, Dimitrije
AU  - Maksimović Zorić, Jelena
AU  - Veljović, Ljubiša
AU  - Milićević, Vesna
PY  - 2022
UR  - https://reponivs.nivs.rs/handle/123456789/587
AB  - У раду је описана појава фурункулозе калифорнијске пастрмке, и поступак
изолације и идентификације узрочника болести, Aeromonas salmonicida subsp.
salmonicida. Током лета 2021. болест се јавила у хроничној форми, са типичним
„фурункулима“ на кожи оболеле рибе и морталитетом од око 15%. Aeromonas
salmonicida subsp. salmonicida је изолован из узорака унутрашњих органа (јетра,
бубрег и слезина) оболелих јединки на триптоза соја подлози и идентификован
конвенционалним микробиолошким методама и секвенцирањем гена 16С
рибозомалне РНК (рРНК).
AB  - This article reports on the occurrence and diagnosis of furunculosis in a rainbow trout.
During summer 2021, the disease occurred in a chronic form, with typical
“furuncules” on the skin of diseased fish and mortality around 15%. The procedure of
isolation and identification of Aeromonas salmonicida subsp. salmonicida, the
causative agent of furunculosis was described. Aeromonas salmonicida subsp.
salmonicida was isolated from internal organ (liver, kidney and spleen) samples on
Trypticase soy agar and identified by conventional microbiology methods and16S
ribosomal RNA (rRNA) gene sequencing.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXIV Simpozijum epizootiologa i epidemiologa (XXIV Epizootiološki dani)
T1  - Furnkuloza kalifornijske pastrmke - prikaz slučaja
T1  - Furunculosis in rainbow trout - case report
EP  - 173
SP  - 172
ER  - 

@conference{
author = "Radosavljevic, Vladimir and Radanović, Oliver and Jezdimirović, Nemanja and Glišić, Dimitrije and Maksimović Zorić, Jelena and Veljović, Ljubiša and Milićević, Vesna",
year = "2022",
abstract = "У раду је описана појава фурункулозе калифорнијске пастрмке, и поступак
изолације и идентификације узрочника болести, Aeromonas salmonicida subsp.
salmonicida. Током лета 2021. болест се јавила у хроничној форми, са типичним
„фурункулима“ на кожи оболеле рибе и морталитетом од око 15%. Aeromonas
salmonicida subsp. salmonicida је изолован из узорака унутрашњих органа (јетра,
бубрег и слезина) оболелих јединки на триптоза соја подлози и идентификован
конвенционалним микробиолошким методама и секвенцирањем гена 16С
рибозомалне РНК (рРНК)., This article reports on the occurrence and diagnosis of furunculosis in a rainbow trout.
During summer 2021, the disease occurred in a chronic form, with typical
“furuncules” on the skin of diseased fish and mortality around 15%. The procedure of
isolation and identification of Aeromonas salmonicida subsp. salmonicida, the
causative agent of furunculosis was described. Aeromonas salmonicida subsp.
salmonicida was isolated from internal organ (liver, kidney and spleen) samples on
Trypticase soy agar and identified by conventional microbiology methods and16S
ribosomal RNA (rRNA) gene sequencing.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXIV Simpozijum epizootiologa i epidemiologa (XXIV Epizootiološki dani)",
title = "Furnkuloza kalifornijske pastrmke - prikaz slučaja, Furunculosis in rainbow trout - case report",
pages = "173-172"
}

Radosavljevic, V., Radanović, O., Jezdimirović, N., Glišić, D., Maksimović Zorić, J., Veljović, L.,& Milićević, V.. (2022). Furnkuloza kalifornijske pastrmke - prikaz slučaja. in XXIV Simpozijum epizootiologa i epidemiologa (XXIV Epizootiološki dani)
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 172-173.

Radosavljevic V, Radanović O, Jezdimirović N, Glišić D, Maksimović Zorić J, Veljović L, Milićević V. Furnkuloza kalifornijske pastrmke - prikaz slučaja. in XXIV Simpozijum epizootiologa i epidemiologa (XXIV Epizootiološki dani). 2022;:172-173..

Radosavljevic, Vladimir, Radanović, Oliver, Jezdimirović, Nemanja, Glišić, Dimitrije, Maksimović Zorić, Jelena, Veljović, Ljubiša, Milićević, Vesna, "Furnkuloza kalifornijske pastrmke - prikaz slučaja" in XXIV Simpozijum epizootiologa i epidemiologa (XXIV Epizootiološki dani) (2022):172-173.