TY  - CONF
AU  - Jezdimirović, Nemanja
AU  - Kureljušić, Branislav
AU  - Savić, Božidar
AU  - Milovanović, Bojan
AU  - Glišić, Dimitrije
AU  - Maletić, Jelena
AU  - Šolaja, Sofija
AU  - Milićević, Vesna
PY  - 2024
UR  - https://reponivs.nivs.rs/handle/123456789/952
AB  - Дивље свиње могу бити резервоари различитих бактерија, вируса и паразита и
могу се пренети на домаће свиње па и на људе индиректним или директним
контактом. Порцине цитомегаловирус (ПЦМВ) препознат је као главни
узрочник атрофичног ринитиса код свиња свих старосних категорија.
Међународни комитет за таксономију вируса (ИТЦВ) је порцине
цитомегаловирус сврстао у suid betaherpesvirus 2 (SuBHV2), и припада
подфамилији Betaherpesvirinae и фамилији Herpesviridae, али није сврстан ни у
један род. Генетска истраживања су показала да је ПЦМВ генетски сроднији
хуманим херпес вирусима (ХХВ) 6А, 6Б и 7. Поред тога, доказано је да ПЦМВ
доприноси репродуктивним проблемима код крмача и резултира раним
неонаталним губицима. Инфекција ПЦМВ-ом је раширена широм света и има
високу преваленцију, посебно у земљама са интензивном производњом свиња.
Клинички пролази непримећено због стеченог имунитета запата, и обично се
јавља код млађих категорија животиња. Нажалост, сама болест и инфекција су
углавном занемарени.
Како бисмо открили улогу дивљих свиња у епидемиологији ПЦМВ-а,
користили смо реал-тиме ПЦР тестирање 50 узорака (лимфни чвор, слезина и
бубрег) пореклом од одстрељених дивљих свиња.
Испитивање је потврдило присуство генома ПЦМВ у 4/50 (8%) узорака. Ови
резултати указују на релативно ниску али не и занемарљиву преваленцију
вируса у популацији дивљих свиња, што може да представља значајан ризик и
за популацију домаћих свиња.
AB  - Wild boars can be reservoirs of various bacteria, viruses, and parasites and can be
transmitted to domestic pigs and even humans through indirect or direct contact.
Porcine cytomegalovirus (PCMV) is recognized as the main cause of atrophic rhinitis
in pigs of all age categories. The International Committee on Taxonomy of Viruses
(ICTV) has classified porcine cytomegalovirus into suid betaherpesvirus 2
(SuBHV2), belonging to the subfamily Betaherpesvirinae and family Herpesviridae,
but it is not classified into any genus. Genetic research has shown that PCMV is
genetically closer to human herpes viruses (HHV) 6A, 6B, and 7. Additionally, it has
been proven that PCMV contributes to reproductive problems in sows and results in
early neonatal losses. PCMV infection is widespread worldwide and has a high
prevalence, especially in countries with intensive pig production. Clinically, it often
goes unnoticed due to acquired maternal immunity and usually occurs in younger
animal categories. Unfortunately, the disease itself and the infection are largely
neglected.
To discover the role of wild boars in PCMV epidemiology, we used real-time PCR
testing of 50 samples (lymph nodes, spleen, and kidney) originating from shot wild
boars.
Testing confirmed the presence of PCMV genome in 4 out of 50 (8%) samples. These
results indicate a relatively low but not negligible prevalence of the virus in the wild
boar population, which may pose a significant risk to the domestic pig population.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
T1  - Prva molekularna detekcija porcine citomegalovirusa kod divljih svinja u Srbiji
T1  - First molecular detection of porcine cytomegalovirus in wild boars in Serbia
EP  - 79
SP  - 78
ER  - 

@conference{
author = "Jezdimirović, Nemanja and Kureljušić, Branislav and Savić, Božidar and Milovanović, Bojan and Glišić, Dimitrije and Maletić, Jelena and Šolaja, Sofija and Milićević, Vesna",
year = "2024",
abstract = "Дивље свиње могу бити резервоари различитих бактерија, вируса и паразита и
могу се пренети на домаће свиње па и на људе индиректним или директним
контактом. Порцине цитомегаловирус (ПЦМВ) препознат је као главни
узрочник атрофичног ринитиса код свиња свих старосних категорија.
Међународни комитет за таксономију вируса (ИТЦВ) је порцине
цитомегаловирус сврстао у suid betaherpesvirus 2 (SuBHV2), и припада
подфамилији Betaherpesvirinae и фамилији Herpesviridae, али није сврстан ни у
један род. Генетска истраживања су показала да је ПЦМВ генетски сроднији
хуманим херпес вирусима (ХХВ) 6А, 6Б и 7. Поред тога, доказано је да ПЦМВ
доприноси репродуктивним проблемима код крмача и резултира раним
неонаталним губицима. Инфекција ПЦМВ-ом је раширена широм света и има
високу преваленцију, посебно у земљама са интензивном производњом свиња.
Клинички пролази непримећено због стеченог имунитета запата, и обично се
јавља код млађих категорија животиња. Нажалост, сама болест и инфекција су
углавном занемарени.
Како бисмо открили улогу дивљих свиња у епидемиологији ПЦМВ-а,
користили смо реал-тиме ПЦР тестирање 50 узорака (лимфни чвор, слезина и
бубрег) пореклом од одстрељених дивљих свиња.
Испитивање је потврдило присуство генома ПЦМВ у 4/50 (8%) узорака. Ови
резултати указују на релативно ниску али не и занемарљиву преваленцију
вируса у популацији дивљих свиња, што може да представља значајан ризик и
за популацију домаћих свиња., Wild boars can be reservoirs of various bacteria, viruses, and parasites and can be
transmitted to domestic pigs and even humans through indirect or direct contact.
Porcine cytomegalovirus (PCMV) is recognized as the main cause of atrophic rhinitis
in pigs of all age categories. The International Committee on Taxonomy of Viruses
(ICTV) has classified porcine cytomegalovirus into suid betaherpesvirus 2
(SuBHV2), belonging to the subfamily Betaherpesvirinae and family Herpesviridae,
but it is not classified into any genus. Genetic research has shown that PCMV is
genetically closer to human herpes viruses (HHV) 6A, 6B, and 7. Additionally, it has
been proven that PCMV contributes to reproductive problems in sows and results in
early neonatal losses. PCMV infection is widespread worldwide and has a high
prevalence, especially in countries with intensive pig production. Clinically, it often
goes unnoticed due to acquired maternal immunity and usually occurs in younger
animal categories. Unfortunately, the disease itself and the infection are largely
neglected.
To discover the role of wild boars in PCMV epidemiology, we used real-time PCR
testing of 50 samples (lymph nodes, spleen, and kidney) originating from shot wild
boars.
Testing confirmed the presence of PCMV genome in 4 out of 50 (8%) samples. These
results indicate a relatively low but not negligible prevalence of the virus in the wild
boar population, which may pose a significant risk to the domestic pig population.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.",
title = "Prva molekularna detekcija porcine citomegalovirusa kod divljih svinja u Srbiji, First molecular detection of porcine cytomegalovirus in wild boars in Serbia",
pages = "79-78"
}

Jezdimirović, N., Kureljušić, B., Savić, B., Milovanović, B., Glišić, D., Maletić, J., Šolaja, S.,& Milićević, V.. (2024). Prva molekularna detekcija porcine citomegalovirusa kod divljih svinja u Srbiji. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 78-79.

Jezdimirović N, Kureljušić B, Savić B, Milovanović B, Glišić D, Maletić J, Šolaja S, Milićević V. Prva molekularna detekcija porcine citomegalovirusa kod divljih svinja u Srbiji. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.. 2024;:78-79..

Jezdimirović, Nemanja, Kureljušić, Branislav, Savić, Božidar, Milovanović, Bojan, Glišić, Dimitrije, Maletić, Jelena, Šolaja, Sofija, Milićević, Vesna, "Prva molekularna detekcija porcine citomegalovirusa kod divljih svinja u Srbiji" in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god. (2024):78-79.

TY  - CONF
AU  - Maksimović Zorić, Jelena
AU  - Veljović, Ljubiša
AU  - Glišić, Dimitrije
AU  - Kureljušić, Branislav
AU  - Jezdimirović, Nemanja
AU  - Stanojević, Slobodan
AU  - Maletić, Jelena
AU  - Ninković, Milan
AU  - Aksić, Đorđe
AU  - Savić, Božidar
PY  - 2024
UR  - https://reponivs.nivs.rs/handle/123456789/951
AB  - Афричка куга свиња (АКС) је први пут потврђена на територији Републике
Србије у јулу 2019. године, код домаћих свиња на епизоотиолошком подручју
Града Београда. Епизоотиолошким увиђајем је установљено да је неодговорно
понашање људи довело до уноса вируса из околних земаља и ширења заразе. У
првом таласу регистрована су жаришта у три насељена места, а применом
законом прописаних мера, болест је стављена под контролу и одјављена
децембра 2019. године. Упркос поновном уносу вируса из околних земаља и
ширењу заразе у популацијама дивљих и домаћих свиња у североисточним,
централним, источним и јужним деловима земље, ситуација на територији
епизоотиолошког подручја Града Београда у периоду од 2020. до 2022. године
је била стабилна. Поновно избијање заразе десило се 9. јуна 2023. године када
је болест потврђена код угинуле дивље свиње пронађене у ловишту
"Панчевачки рит", на територији општине Палилула. Већ 12. јуна потврђен је и
први случај код екстензивно узгајаних домаћих свиња у насљеном месту
Крњача. Зараза је убрзо потврђена и код дивљих свиња суседног ловишта "Рит"
и домаћих свиња других насељених места општине Палилула (Бесни Фок,
Дунавац, Јабучки Рит, Ковилово, Падинска Скела, Борча, Овча, Сланци, Велико
Село, Вишњица). У периоду од 9. јуна до 5. августа болест је потврђена на 157
газдинстава, односно код 223 домаће и код 110 дивљих свиња, те је посумњано
да су активности људи уз неадекватне биосигурносне мере на газдинствима
допринеле настанку епизоотије. Током јула и августа 2023. године зараза је
потврђена и код дивљих и домаћих свиња на територијама општина Земун,
Гроцка, Чукарица и Обреновац, где је регистровано 34 позитивна газдинстава и
једно ловиште. Од 26. септембра до 28. децембра није било нових случајева
заразе на епизоотиолошком подручју Града Београда, те је зараза одјављена 26.
децембра, односно 3 месеца после последњег потврђеног случаја АКС.
AB  - African swine fever (ASF) was first confirmed in the Republic of Serbia in July 2019,
in domestic pigs settled within the Belgrade epizootiology area. Through an
epizootiological investigation, it was concluded that the outbreak was a result of
irresponsible human behaviour which led to the introduction of the virus from
surrounding countries and its further spread. In the first wave, three outbreaks were
detected in three villages and by implementing regulatory measures for disease
control, the infection was successfully resolved in December 2019. Although the virus
re-entered from neighboring countries and spread across the northeast, central,
eastern, and southern regions, there were no new disease outbreaks in the Belgrade
epizootiological unit during the 2020-2022 period. Despite efforts to control the
infection, the disease reappeared in June 2023, spreading rapidly among wild boars
and domestic pigs in various settlements and hunting grounds of Palilula municipality.
During the two-month period, the disease was confirmed on 157 holdings,
respectively in 223 domestic pigs and 110 wild boars, implying the significance of
human behaviour and poor biosecurity measures on the epizootic spread of the
disease. During July and August 2023, the infection was confirmed in wild boars and
domestic pigs in the municipalities Zemun, Grocka, Čukarica, and Obrenovac, where
34 holdings and one hunting ground were declared positive. From September 26th to
December 28th, there were no new cases of infection in the Belgrade epizootiology
area, and the infection was resolved on December 26th, i.e. 3 months after the last
confirmed case of ASF.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
T1  - Afrička kuga svinja - epizootiološka situacija na teritoriji okruga grada Beograda u periodu od 2019. do 2023. godine
T1  - African swine fever - epizootiological situation in the Belgrade city area in the period from 2019 to 2023
EP  - 91
SP  - 90
ER  - 

@conference{
author = "Maksimović Zorić, Jelena and Veljović, Ljubiša and Glišić, Dimitrije and Kureljušić, Branislav and Jezdimirović, Nemanja and Stanojević, Slobodan and Maletić, Jelena and Ninković, Milan and Aksić, Đorđe and Savić, Božidar",
year = "2024",
abstract = "Афричка куга свиња (АКС) је први пут потврђена на територији Републике
Србије у јулу 2019. године, код домаћих свиња на епизоотиолошком подручју
Града Београда. Епизоотиолошким увиђајем је установљено да је неодговорно
понашање људи довело до уноса вируса из околних земаља и ширења заразе. У
првом таласу регистрована су жаришта у три насељена места, а применом
законом прописаних мера, болест је стављена под контролу и одјављена
децембра 2019. године. Упркос поновном уносу вируса из околних земаља и
ширењу заразе у популацијама дивљих и домаћих свиња у североисточним,
централним, источним и јужним деловима земље, ситуација на територији
епизоотиолошког подручја Града Београда у периоду од 2020. до 2022. године
је била стабилна. Поновно избијање заразе десило се 9. јуна 2023. године када
је болест потврђена код угинуле дивље свиње пронађене у ловишту
"Панчевачки рит", на територији општине Палилула. Већ 12. јуна потврђен је и
први случај код екстензивно узгајаних домаћих свиња у насљеном месту
Крњача. Зараза је убрзо потврђена и код дивљих свиња суседног ловишта "Рит"
и домаћих свиња других насељених места општине Палилула (Бесни Фок,
Дунавац, Јабучки Рит, Ковилово, Падинска Скела, Борча, Овча, Сланци, Велико
Село, Вишњица). У периоду од 9. јуна до 5. августа болест је потврђена на 157
газдинстава, односно код 223 домаће и код 110 дивљих свиња, те је посумњано
да су активности људи уз неадекватне биосигурносне мере на газдинствима
допринеле настанку епизоотије. Током јула и августа 2023. године зараза је
потврђена и код дивљих и домаћих свиња на територијама општина Земун,
Гроцка, Чукарица и Обреновац, где је регистровано 34 позитивна газдинстава и
једно ловиште. Од 26. септембра до 28. децембра није било нових случајева
заразе на епизоотиолошком подручју Града Београда, те је зараза одјављена 26.
децембра, односно 3 месеца после последњег потврђеног случаја АКС., African swine fever (ASF) was first confirmed in the Republic of Serbia in July 2019,
in domestic pigs settled within the Belgrade epizootiology area. Through an
epizootiological investigation, it was concluded that the outbreak was a result of
irresponsible human behaviour which led to the introduction of the virus from
surrounding countries and its further spread. In the first wave, three outbreaks were
detected in three villages and by implementing regulatory measures for disease
control, the infection was successfully resolved in December 2019. Although the virus
re-entered from neighboring countries and spread across the northeast, central,
eastern, and southern regions, there were no new disease outbreaks in the Belgrade
epizootiological unit during the 2020-2022 period. Despite efforts to control the
infection, the disease reappeared in June 2023, spreading rapidly among wild boars
and domestic pigs in various settlements and hunting grounds of Palilula municipality.
During the two-month period, the disease was confirmed on 157 holdings,
respectively in 223 domestic pigs and 110 wild boars, implying the significance of
human behaviour and poor biosecurity measures on the epizootic spread of the
disease. During July and August 2023, the infection was confirmed in wild boars and
domestic pigs in the municipalities Zemun, Grocka, Čukarica, and Obrenovac, where
34 holdings and one hunting ground were declared positive. From September 26th to
December 28th, there were no new cases of infection in the Belgrade epizootiology
area, and the infection was resolved on December 26th, i.e. 3 months after the last
confirmed case of ASF.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.",
title = "Afrička kuga svinja - epizootiološka situacija na teritoriji okruga grada Beograda u periodu od 2019. do 2023. godine, African swine fever - epizootiological situation in the Belgrade city area in the period from 2019 to 2023",
pages = "91-90"
}

Maksimović Zorić, J., Veljović, L., Glišić, D., Kureljušić, B., Jezdimirović, N., Stanojević, S., Maletić, J., Ninković, M., Aksić, Đ.,& Savić, B.. (2024). Afrička kuga svinja - epizootiološka situacija na teritoriji okruga grada Beograda u periodu od 2019. do 2023. godine. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 90-91.

Maksimović Zorić J, Veljović L, Glišić D, Kureljušić B, Jezdimirović N, Stanojević S, Maletić J, Ninković M, Aksić Đ, Savić B. Afrička kuga svinja - epizootiološka situacija na teritoriji okruga grada Beograda u periodu od 2019. do 2023. godine. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.. 2024;:90-91..

Maksimović Zorić, Jelena, Veljović, Ljubiša, Glišić, Dimitrije, Kureljušić, Branislav, Jezdimirović, Nemanja, Stanojević, Slobodan, Maletić, Jelena, Ninković, Milan, Aksić, Đorđe, Savić, Božidar, "Afrička kuga svinja - epizootiološka situacija na teritoriji okruga grada Beograda u periodu od 2019. do 2023. godine" in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god. (2024):90-91.

TY  - CONF
AU  - Kureljušić, Branislav
AU  - Vićić, Ivan
AU  - Bradić, Milijana
AU  - Maletić, Jelena
AU  - Jezdimirović, Nemanja
AU  - Savić, Božidar
AU  - Milovanović, Bojan
AU  - Karabasil, Neđeljko
PY  - 2024
UR  - https://reponivs.nivs.rs/handle/123456789/955
AB  - Интензивирањем бројлерске производње дошло је до значајног повећања
преваленције различитих патолошких стања чија основна етиологија почива у
самом технолошком процесу производње. Појава контактног дерматитиса на
плантарној кожи екстремитета („foot pad dermatitis“), затим на скочном зглобу
(„hock burns“) и на грудима („breast burns“) данас представља врло раширену
појаву у интензивној производњи, наносећи значајне економске губитке услед
смањеног прираста и одбацивања трупова на линији клања. Поред интензивирања
бројлерске производње значајан утицај на њихову појаву имају исхрана, услови и
начин држања (врста простирке) и генетска предиспозиција. Најзначајнији
фактор ризика за настанак лезија је влажна простирка посебно у комбинацији са
штетним дејством амонијака. Појава лезија има веома велики значај као
индикатор добробити имајући у виду да постоје претпоставке да су установљене
лезије врло болне и да често онемогућавају нормално кретање пилића.
Макроскопским прегледом лезија на плантарној површини уочавају се
инфламаторне лезије праћене некрозом на јастучићима табана и/или на прстима.
Лезије се ређе могу установити на тибиометатарзалним зглобовима. Код благог
облика постоји дисколорација на кожи, хиперкератоза, а у тежим случајевима
јавња се едем, ерозије и улцерације.
Хистопатолошки, благе лезије се карактеришу умереном хиперплазијом и/или
хиперкератозом епидермиса, конгестијом и едемом, уз присуство благог
инфламаторног инфилтрата у форми периваскулитиса и субепителијалне
инфилтрације мононуклеарним ћелијама и мањим бројем гранулоцита. Умерене
суперфицијелне лезије се карактеришу врло израженом хиперплазијом и
хиперкератозом епидермиса, егзоцитозом, појавом пустула и красти, конгестијом
и инфилтрацијом дермиса мононуклеарним и гранулоцитиним инфилтратом.
Тешке, дубоке лезије се карактеришу некрозом епидермиса који је замењен
некротичним гнојним садржајем, појавом гранулационог ткива, израженом
инфламаторном инфилтрацијом (периваскулитис и субепителна инфилтрација)
доминантно хетерофилним гранулоцитима и мононуклеарним ћелијама.
Имајући у виду да је појава контактног дерматитиса све учесталија појава како на
фармама бројлерских пилића тако и на кланици приликом прегледа трупова на
линија клања неопходно је имплементирати програм контроле овог патолошког
стања и на тај начин побољшати добробит и обезбедити производњу квалитетног
и безбедног пилећег меса.
AB  - The intensification of broiler production has led to a significant increase in the
prevalence of various pathological conditions, whose basic etiology lies in the
production process itself. The occurrence of contact dermatitis on the plantar skin of
extremities ("foot pad dermatitis"), then on the hock joint ("hock burns"), and on the
breasts ("breast burns") is today a very widespread phenomenon in intensive production,
causing significant economic losses due to reduced growth and carcass rejection on the
slaughter line. In addition to the intensification of broiler production, significant factors
influencing their occurrence are nutrition, housing conditions (type of bedding), and
genetic predisposition. The most significant risk factor for lesion formation is wet
bedding, especially in combination with the harmful effects of ammonia. The
occurrence of lesions is very important as an indicator of welfare, considering that there
are assumptions that established lesions are very painful and often prevent normal
chicken movement. Macroscopic examination of lesions on the plantar surface reveals
inflammatory lesions accompanied by necrosis on the foot pads and/or toes. Lesions are
less commonly found on the tibiotarsal joints. In mild forms, discoloration of the skin,
hyperkeratosis, and in severe cases, edema, erosions, and ulcerations occur.
Histopathologically, mild lesions are characterized by moderate epidermal hyperplasia
and/or hyperkeratosis, congestion and edema, with the presence of mild inflammatory
infiltrate in the form of perivasculitis and subepithelial infiltration of mononuclear cells
and a small number of granulocytes. Moderate superficial lesions are characterized by
very pronounced epidermal hyperplasia and hyperkeratosis, exocytosis, appearance of
pustules and crusts, congestion, and infiltration of the dermis with mononuclear and
granulocytic infiltrate. Severe, deep lesions are characterized by epidermal necrosis
replaced by necrotic purulent content, appearance of granulation tissue, pronounced
inflammatory infiltration (perivasculitis and subepithelial infiltration) predominantly
with heterophilic granulocytes and mononuclear cells. Considering that the occurrence
of contact dermatitis is becoming more frequent both on broiler farms and in
slaughterhouses during carcass examination on the slaughter line, it is necessary to
implement a control program for this pathological condition
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
T1  - Kontaktni pododermatitis kod brojlerskih pilića – patološki aspekt -
T1  - Contact pododermatitis in broiler chickens – pathological aspect –
EP  - 147
SP  - 146
ER  - 

@conference{
author = "Kureljušić, Branislav and Vićić, Ivan and Bradić, Milijana and Maletić, Jelena and Jezdimirović, Nemanja and Savić, Božidar and Milovanović, Bojan and Karabasil, Neđeljko",
year = "2024",
abstract = "Интензивирањем бројлерске производње дошло је до значајног повећања
преваленције различитих патолошких стања чија основна етиологија почива у
самом технолошком процесу производње. Појава контактног дерматитиса на
плантарној кожи екстремитета („foot pad dermatitis“), затим на скочном зглобу
(„hock burns“) и на грудима („breast burns“) данас представља врло раширену
појаву у интензивној производњи, наносећи значајне економске губитке услед
смањеног прираста и одбацивања трупова на линији клања. Поред интензивирања
бројлерске производње значајан утицај на њихову појаву имају исхрана, услови и
начин држања (врста простирке) и генетска предиспозиција. Најзначајнији
фактор ризика за настанак лезија је влажна простирка посебно у комбинацији са
штетним дејством амонијака. Појава лезија има веома велики значај као
индикатор добробити имајући у виду да постоје претпоставке да су установљене
лезије врло болне и да често онемогућавају нормално кретање пилића.
Макроскопским прегледом лезија на плантарној површини уочавају се
инфламаторне лезије праћене некрозом на јастучићима табана и/или на прстима.
Лезије се ређе могу установити на тибиометатарзалним зглобовима. Код благог
облика постоји дисколорација на кожи, хиперкератоза, а у тежим случајевима
јавња се едем, ерозије и улцерације.
Хистопатолошки, благе лезије се карактеришу умереном хиперплазијом и/или
хиперкератозом епидермиса, конгестијом и едемом, уз присуство благог
инфламаторног инфилтрата у форми периваскулитиса и субепителијалне
инфилтрације мононуклеарним ћелијама и мањим бројем гранулоцита. Умерене
суперфицијелне лезије се карактеришу врло израженом хиперплазијом и
хиперкератозом епидермиса, егзоцитозом, појавом пустула и красти, конгестијом
и инфилтрацијом дермиса мононуклеарним и гранулоцитиним инфилтратом.
Тешке, дубоке лезије се карактеришу некрозом епидермиса који је замењен
некротичним гнојним садржајем, појавом гранулационог ткива, израженом
инфламаторном инфилтрацијом (периваскулитис и субепителна инфилтрација)
доминантно хетерофилним гранулоцитима и мононуклеарним ћелијама.
Имајући у виду да је појава контактног дерматитиса све учесталија појава како на
фармама бројлерских пилића тако и на кланици приликом прегледа трупова на
линија клања неопходно је имплементирати програм контроле овог патолошког
стања и на тај начин побољшати добробит и обезбедити производњу квалитетног
и безбедног пилећег меса., The intensification of broiler production has led to a significant increase in the
prevalence of various pathological conditions, whose basic etiology lies in the
production process itself. The occurrence of contact dermatitis on the plantar skin of
extremities ("foot pad dermatitis"), then on the hock joint ("hock burns"), and on the
breasts ("breast burns") is today a very widespread phenomenon in intensive production,
causing significant economic losses due to reduced growth and carcass rejection on the
slaughter line. In addition to the intensification of broiler production, significant factors
influencing their occurrence are nutrition, housing conditions (type of bedding), and
genetic predisposition. The most significant risk factor for lesion formation is wet
bedding, especially in combination with the harmful effects of ammonia. The
occurrence of lesions is very important as an indicator of welfare, considering that there
are assumptions that established lesions are very painful and often prevent normal
chicken movement. Macroscopic examination of lesions on the plantar surface reveals
inflammatory lesions accompanied by necrosis on the foot pads and/or toes. Lesions are
less commonly found on the tibiotarsal joints. In mild forms, discoloration of the skin,
hyperkeratosis, and in severe cases, edema, erosions, and ulcerations occur.
Histopathologically, mild lesions are characterized by moderate epidermal hyperplasia
and/or hyperkeratosis, congestion and edema, with the presence of mild inflammatory
infiltrate in the form of perivasculitis and subepithelial infiltration of mononuclear cells
and a small number of granulocytes. Moderate superficial lesions are characterized by
very pronounced epidermal hyperplasia and hyperkeratosis, exocytosis, appearance of
pustules and crusts, congestion, and infiltration of the dermis with mononuclear and
granulocytic infiltrate. Severe, deep lesions are characterized by epidermal necrosis
replaced by necrotic purulent content, appearance of granulation tissue, pronounced
inflammatory infiltration (perivasculitis and subepithelial infiltration) predominantly
with heterophilic granulocytes and mononuclear cells. Considering that the occurrence
of contact dermatitis is becoming more frequent both on broiler farms and in
slaughterhouses during carcass examination on the slaughter line, it is necessary to
implement a control program for this pathological condition",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.",
title = "Kontaktni pododermatitis kod brojlerskih pilića – patološki aspekt -, Contact pododermatitis in broiler chickens – pathological aspect –",
pages = "147-146"
}

Kureljušić, B., Vićić, I., Bradić, M., Maletić, J., Jezdimirović, N., Savić, B., Milovanović, B.,& Karabasil, N.. (2024). Kontaktni pododermatitis kod brojlerskih pilića – patološki aspekt -. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 146-147.

Kureljušić B, Vićić I, Bradić M, Maletić J, Jezdimirović N, Savić B, Milovanović B, Karabasil N. Kontaktni pododermatitis kod brojlerskih pilića – patološki aspekt -. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.. 2024;:146-147..

Kureljušić, Branislav, Vićić, Ivan, Bradić, Milijana, Maletić, Jelena, Jezdimirović, Nemanja, Savić, Božidar, Milovanović, Bojan, Karabasil, Neđeljko, "Kontaktni pododermatitis kod brojlerskih pilića – patološki aspekt -" in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god. (2024):146-147.

TY  - CONF
AU  - Šolaja, Sofija
AU  - Glišić, Dimitrije
AU  - Zurovac Sapundžić, Zorana
AU  - Milovanović, Bojan
AU  - Maletić, Jelena
AU  - Jezdimirović, Nemanja
AU  - Kureljušić, Branislav
AU  - Milićević, Vesna
PY  - 2024
UR  - https://reponivs.nivs.rs/handle/123456789/954
AB  - Инфективне болести представљају један од главних проблема у интензивном узгоју свиња, упркос технолошком напретку и развоју биосигурносних мера. Вирусна респираторна обољења која имају највећи економски и здравствени значај у свињарству су репродуктивни и респираторни синдром свиња, инфлуенца свиња и Аујецкијева болест. Сеоска газдинства екстензивног типа, и даље предcтављају најчешћи начин узгоја свиња у Србији. Циљ истраживања је био да се утврди серопреваленција респираторних вируса на сеоским газдинствима која представљају извор патогена за комерцијалне фарме. Крвни серуми су узорковани од 222 јединке са 69 газдинстава на којима се налазило до 10 свиња. За детекцију антитела су коришћени комерцијални ЕЛИСА тестови према упутству произвођача. Установљено је 38 (17,1%) серопозитивних јединки на вирус Аујецкијеве болести и 6 (2,7%) на вирус репродуктивног и респираторног синдрома. Антитела на вирус инфлуенце свиња нису установљена ни код једне јединкe. Два газдинства (2,9%) су била серопозитивна на репродуктивни и респираторни синдром са серопреваленцијом у запату од 100%, без присуства антитела на друге тестиране вирусе. Проценат серопозитивних запата на Аујецкијеву болест је био 10,1%, а серопреваленција у запатима је варирала од 33% до 100%. Серонегативних запата на све три болести је било 48 (69,5%). Ови резултати указују да међу свињама на сеоским газдинствима највише циркулише вирус Аујецкијеве болести. Главне катактеристике сеоских газдинстава су неадекватне биосигурносне мере, исхрана помијама и неконтролисан транспорт и контакт са другим врстама животиња. Стога, будући да је здравствени статус оваквих запата непознат, они се сматрају извором високог ризика за комерцијалне фарме. Власници сеоских газдинстава су често запослени на комерцијалним фармама, без обзира на забрану држања свиња, и представљају везу између сеоских газдинстава и комерцијалних фарми. Неопходно је спровести мониторниг здравственог стања, побољшање биосигурносних мера и едукацију власника како би се спречило ширење инфективних обољења са сеоских газдинстава на комерцијалне фарме.
AB  - Infectious diseases represent one of the main challenges in intensive pig farming, despite technological advancements and biosecurity improvement. Viral respiratory diseases with the greatest economic and health significance in pig farming include porcine reproductive and respiratory syndrome (PRRS), swine influenza (SI), and Aujeszky's disease (AD). Small backyard farms continue to be the most common method of pig farming in Serbia. This research aimed to assess the prevalence of respiratory viruses on backyard farms, which serve as a source of pathogens for commercial farms. Serum samples were collected from 222 individuals on 69 farms, each housing up to 10 pigs. Commercial ELISA tests were used for antibody detection according to the manufacturer's instructions. Out of the samples, 38 (17.1%) were seropositive for AD, and 6 (2.7%) for PRRS. No antibodies to the swine influenza virus were detected. Two farms (2.9%) were seropositive for PRRS with a herd-level seroprevalence of 100%, with no antibodies detected for other tested viruses. The percentage of seropositive farms for Aujeszky's disease was 10.1%, with herd-level seroprevalence ranging from 33% to 100%. There were 48 (69.5%) seronegative herds for all three diseases. These results indicate that Aujeszky's disease virus circulates the most among pigs on backyard farms. Main characteristics of backyard farms are low biosecurity, swill feeding, uncontrolled trade and contact with other animal species. Thus, since the health status of backyard farms is unknown, they are considered a high-risk infection source for commercial farms. Owners of backyard farms are often employed on commercial farms, despite regulations, serving as a link between backyard and commercial farms. Implementation of monitoring, improvement of biosecurity measures and education of owners are crucial steps for preventing the transmission of infectious diseases between backyard and commercial farms.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
T1  - Seroprevalencija virusnih respiratornih oboljenja svinja na seoskim gazdinstvima u Srbiji
T1  - Seroprevalence of viral respiratory diseases in swine backyard farms in Serbia
EP  - 107
SP  - 106
ER  - 

@conference{
author = "Šolaja, Sofija and Glišić, Dimitrije and Zurovac Sapundžić, Zorana and Milovanović, Bojan and Maletić, Jelena and Jezdimirović, Nemanja and Kureljušić, Branislav and Milićević, Vesna",
year = "2024",
abstract = "Инфективне болести представљају један од главних проблема у интензивном узгоју свиња, упркос технолошком напретку и развоју биосигурносних мера. Вирусна респираторна обољења која имају највећи економски и здравствени значај у свињарству су репродуктивни и респираторни синдром свиња, инфлуенца свиња и Аујецкијева болест. Сеоска газдинства екстензивног типа, и даље предcтављају најчешћи начин узгоја свиња у Србији. Циљ истраживања је био да се утврди серопреваленција респираторних вируса на сеоским газдинствима која представљају извор патогена за комерцијалне фарме. Крвни серуми су узорковани од 222 јединке са 69 газдинстава на којима се налазило до 10 свиња. За детекцију антитела су коришћени комерцијални ЕЛИСА тестови према упутству произвођача. Установљено је 38 (17,1%) серопозитивних јединки на вирус Аујецкијеве болести и 6 (2,7%) на вирус репродуктивног и респираторног синдрома. Антитела на вирус инфлуенце свиња нису установљена ни код једне јединкe. Два газдинства (2,9%) су била серопозитивна на репродуктивни и респираторни синдром са серопреваленцијом у запату од 100%, без присуства антитела на друге тестиране вирусе. Проценат серопозитивних запата на Аујецкијеву болест је био 10,1%, а серопреваленција у запатима је варирала од 33% до 100%. Серонегативних запата на све три болести је било 48 (69,5%). Ови резултати указују да међу свињама на сеоским газдинствима највише циркулише вирус Аујецкијеве болести. Главне катактеристике сеоских газдинстава су неадекватне биосигурносне мере, исхрана помијама и неконтролисан транспорт и контакт са другим врстама животиња. Стога, будући да је здравствени статус оваквих запата непознат, они се сматрају извором високог ризика за комерцијалне фарме. Власници сеоских газдинстава су често запослени на комерцијалним фармама, без обзира на забрану држања свиња, и представљају везу између сеоских газдинстава и комерцијалних фарми. Неопходно је спровести мониторниг здравственог стања, побољшање биосигурносних мера и едукацију власника како би се спречило ширење инфективних обољења са сеоских газдинстава на комерцијалне фарме., Infectious diseases represent one of the main challenges in intensive pig farming, despite technological advancements and biosecurity improvement. Viral respiratory diseases with the greatest economic and health significance in pig farming include porcine reproductive and respiratory syndrome (PRRS), swine influenza (SI), and Aujeszky's disease (AD). Small backyard farms continue to be the most common method of pig farming in Serbia. This research aimed to assess the prevalence of respiratory viruses on backyard farms, which serve as a source of pathogens for commercial farms. Serum samples were collected from 222 individuals on 69 farms, each housing up to 10 pigs. Commercial ELISA tests were used for antibody detection according to the manufacturer's instructions. Out of the samples, 38 (17.1%) were seropositive for AD, and 6 (2.7%) for PRRS. No antibodies to the swine influenza virus were detected. Two farms (2.9%) were seropositive for PRRS with a herd-level seroprevalence of 100%, with no antibodies detected for other tested viruses. The percentage of seropositive farms for Aujeszky's disease was 10.1%, with herd-level seroprevalence ranging from 33% to 100%. There were 48 (69.5%) seronegative herds for all three diseases. These results indicate that Aujeszky's disease virus circulates the most among pigs on backyard farms. Main characteristics of backyard farms are low biosecurity, swill feeding, uncontrolled trade and contact with other animal species. Thus, since the health status of backyard farms is unknown, they are considered a high-risk infection source for commercial farms. Owners of backyard farms are often employed on commercial farms, despite regulations, serving as a link between backyard and commercial farms. Implementation of monitoring, improvement of biosecurity measures and education of owners are crucial steps for preventing the transmission of infectious diseases between backyard and commercial farms.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.",
title = "Seroprevalencija virusnih respiratornih oboljenja svinja na seoskim gazdinstvima u Srbiji, Seroprevalence of viral respiratory diseases in swine backyard farms in Serbia",
pages = "107-106"
}

Šolaja, S., Glišić, D., Zurovac Sapundžić, Z., Milovanović, B., Maletić, J., Jezdimirović, N., Kureljušić, B.,& Milićević, V.. (2024). Seroprevalencija virusnih respiratornih oboljenja svinja na seoskim gazdinstvima u Srbiji. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 106-107.

Šolaja S, Glišić D, Zurovac Sapundžić Z, Milovanović B, Maletić J, Jezdimirović N, Kureljušić B, Milićević V. Seroprevalencija virusnih respiratornih oboljenja svinja na seoskim gazdinstvima u Srbiji. in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god.. 2024;:106-107..

Šolaja, Sofija, Glišić, Dimitrije, Zurovac Sapundžić, Zorana, Milovanović, Bojan, Maletić, Jelena, Jezdimirović, Nemanja, Kureljušić, Branislav, Milićević, Vesna, "Seroprevalencija virusnih respiratornih oboljenja svinja na seoskim gazdinstvima u Srbiji" in XXVI Simpozijum epizootiologa i epidemiolooga (XXVI Epizootilološki dani), Banja Koviljača  10 - 12. april 2024. god. (2024):106-107.

TY  - CONF
AU  - Pajić, Marko
AU  - Knežević, Slobodan
AU  - Maletić, Jelena
AU  - Spiridonović, Sava
AU  - Đurđević, Biljana
AU  - Todorović, Dalibor
AU  - Ostojić Andrić, Dušica
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/755
AB  - The broiler production faces many challenges, which can cause negative 
effects on their health and welfare. The great importance for farmers is to prevent 
disease outbreaks, and biosecurity measures are very significant. This study aimed 
to quantify the level of biosecurity measures in broiler farms of different capacities, 
using a standardized procedure, and to identify key aspects that would require 
improvements. The research was conducted from May to September 2022, and 15 
randomly selected broiler farms participated. Five large-size (>30,000 chickens), 
five middle-size (10,000-30,000 chickens) and five small-size farms (<10,000 
chickens) were analyzed. All farms are located in Vojvodina and farmers agreed to 
participate in the survey. The Biocheck.UGent scoring system 
(https://biocheckgent.com/en) was used to quantify biosecurity measures. The 
overall farm biosecurity is a weighted average of the external and internal 
biosecurity. Our results showed a low level of implementation of internal and 
external biosecurity measures on all farms (40-63%). The overall rating of 
biosecurity on farms was lower than the world and country's average. The results of 
this study suggest that the control of implemented biosecurity measures in broiler 
farms is very important. Most of the biosecurity risks for broiler farms originate 
from inappropriate site selection, purchase of day-old chicks of unknown quality, 
lack of procedures, and training of farm employees. This study should provide a 
good encouragement for the development of a biosecurity plan, identifying risks 
and the appropriate way to educate farm owners, as well as farm employees, on the 
implementation of biosecurity measures.
PB  - Belgrade : Institute for Animal Husbandry
C3  - Proceedings of the 14th International Symposium  Modern Trends in Livestock Production, 4-6 October, Belgrade
T1  - Assessment of the current state of biosecurity measures on broiler chicken farms with different capacities In Vojvodina
EP  - 205
SP  - 193
ER  - 

@conference{
author = "Pajić, Marko and Knežević, Slobodan and Maletić, Jelena and Spiridonović, Sava and Đurđević, Biljana and Todorović, Dalibor and Ostojić Andrić, Dušica",
year = "2023",
abstract = "The broiler production faces many challenges, which can cause negative 
effects on their health and welfare. The great importance for farmers is to prevent 
disease outbreaks, and biosecurity measures are very significant. This study aimed 
to quantify the level of biosecurity measures in broiler farms of different capacities, 
using a standardized procedure, and to identify key aspects that would require 
improvements. The research was conducted from May to September 2022, and 15 
randomly selected broiler farms participated. Five large-size (>30,000 chickens), 
five middle-size (10,000-30,000 chickens) and five small-size farms (<10,000 
chickens) were analyzed. All farms are located in Vojvodina and farmers agreed to 
participate in the survey. The Biocheck.UGent scoring system 
(https://biocheckgent.com/en) was used to quantify biosecurity measures. The 
overall farm biosecurity is a weighted average of the external and internal 
biosecurity. Our results showed a low level of implementation of internal and 
external biosecurity measures on all farms (40-63%). The overall rating of 
biosecurity on farms was lower than the world and country's average. The results of 
this study suggest that the control of implemented biosecurity measures in broiler 
farms is very important. Most of the biosecurity risks for broiler farms originate 
from inappropriate site selection, purchase of day-old chicks of unknown quality, 
lack of procedures, and training of farm employees. This study should provide a 
good encouragement for the development of a biosecurity plan, identifying risks 
and the appropriate way to educate farm owners, as well as farm employees, on the 
implementation of biosecurity measures.",
publisher = "Belgrade : Institute for Animal Husbandry",
journal = "Proceedings of the 14th International Symposium  Modern Trends in Livestock Production, 4-6 October, Belgrade",
title = "Assessment of the current state of biosecurity measures on broiler chicken farms with different capacities In Vojvodina",
pages = "205-193"
}

Pajić, M., Knežević, S., Maletić, J., Spiridonović, S., Đurđević, B., Todorović, D.,& Ostojić Andrić, D.. (2023). Assessment of the current state of biosecurity measures on broiler chicken farms with different capacities In Vojvodina. in Proceedings of the 14th International Symposium  Modern Trends in Livestock Production, 4-6 October, Belgrade
Belgrade : Institute for Animal Husbandry., 193-205.

Pajić M, Knežević S, Maletić J, Spiridonović S, Đurđević B, Todorović D, Ostojić Andrić D. Assessment of the current state of biosecurity measures on broiler chicken farms with different capacities In Vojvodina. in Proceedings of the 14th International Symposium  Modern Trends in Livestock Production, 4-6 October, Belgrade. 2023;:193-205..

Pajić, Marko, Knežević, Slobodan, Maletić, Jelena, Spiridonović, Sava, Đurđević, Biljana, Todorović, Dalibor, Ostojić Andrić, Dušica, "Assessment of the current state of biosecurity measures on broiler chicken farms with different capacities In Vojvodina" in Proceedings of the 14th International Symposium  Modern Trends in Livestock Production, 4-6 October, Belgrade (2023):193-205.

TY  - CONF
AU  - Maletić, Jelena
AU  - Pajić, Marko
AU  - Đurđević, Biljana
AU  - Kureljušić, Branislav
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/685
AB  - The World Organisation for Animal Health (WOAH, founded as OIE) is an international, intergovernmental organization responsible for standard-setting processes on animal health and welfare since its establishment in 1924. Terrestrial and Aquatic Animal Health Codes of WOAH provide internationally recognized science-based standards for improving animal health and welfare and veterinary public health worldwide, including through means for safe international trade in terrestrial and aquatic animals and their products. The Manuals of Diagnostic Tests and Vaccines for Terrestrial and Aquatic Animals provide a standardized approach to diagnosing the diseases listed in the Terrestrial and Aquatic Codes.
For the WOAH and its 182 Members, biosecurity is defined as “a set of management and physical measures designed to reduce the risk of introduction, establishment and spread of animal diseases, infections or infestations to, from and within an animal population”. Although there is no specific chapter on biosecurity in WOAH international standard yet, the term biosecurity is mentioned 264 times across 12 sections and 45 chapters of Terrestrial Code, which recognizes the importance of biosecurity in the whole veterinary domain: for prevention and control of animal diseases, improving animal health and protecting public health, for animal welfare purposes, safe trade of live animals and veterinary commodities, etc.
However, WOAH recognizes biosecurity as essential for disease prevention and control, thereby improving animal health and welfare. Therefore, an ad hoc group of recognized international experts has been designated by the Director General of WOAH to start drafting a specific chapter on biosecurity. This work will follow WOAH’s standards-setting process which includes consultation with WOAH Members before being proposed for adoption by the World Assembly of Delegates at the WOAH General Assembly. The role of the scientific community and national research entities is key for developing further these standards and their implementation by Members.
PB  - Ghent : Faculty of veterinary medicine
C3  - COST action CA20103 "Biosecurity Enhanced Through Training Evaluation and Raising Awareness"
T1  - Biosecurity measures on the broilers and breeders' farms in Serbia
EP  - 31
SP  - 31
ER  - 

@conference{
author = "Maletić, Jelena and Pajić, Marko and Đurđević, Biljana and Kureljušić, Branislav",
year = "2023",
abstract = "The World Organisation for Animal Health (WOAH, founded as OIE) is an international, intergovernmental organization responsible for standard-setting processes on animal health and welfare since its establishment in 1924. Terrestrial and Aquatic Animal Health Codes of WOAH provide internationally recognized science-based standards for improving animal health and welfare and veterinary public health worldwide, including through means for safe international trade in terrestrial and aquatic animals and their products. The Manuals of Diagnostic Tests and Vaccines for Terrestrial and Aquatic Animals provide a standardized approach to diagnosing the diseases listed in the Terrestrial and Aquatic Codes.
For the WOAH and its 182 Members, biosecurity is defined as “a set of management and physical measures designed to reduce the risk of introduction, establishment and spread of animal diseases, infections or infestations to, from and within an animal population”. Although there is no specific chapter on biosecurity in WOAH international standard yet, the term biosecurity is mentioned 264 times across 12 sections and 45 chapters of Terrestrial Code, which recognizes the importance of biosecurity in the whole veterinary domain: for prevention and control of animal diseases, improving animal health and protecting public health, for animal welfare purposes, safe trade of live animals and veterinary commodities, etc.
However, WOAH recognizes biosecurity as essential for disease prevention and control, thereby improving animal health and welfare. Therefore, an ad hoc group of recognized international experts has been designated by the Director General of WOAH to start drafting a specific chapter on biosecurity. This work will follow WOAH’s standards-setting process which includes consultation with WOAH Members before being proposed for adoption by the World Assembly of Delegates at the WOAH General Assembly. The role of the scientific community and national research entities is key for developing further these standards and their implementation by Members.",
publisher = "Ghent : Faculty of veterinary medicine",
journal = "COST action CA20103 "Biosecurity Enhanced Through Training Evaluation and Raising Awareness"",
title = "Biosecurity measures on the broilers and breeders' farms in Serbia",
pages = "31-31"
}

Maletić, J., Pajić, M., Đurđević, B.,& Kureljušić, B.. (2023). Biosecurity measures on the broilers and breeders' farms in Serbia. in COST action CA20103 "Biosecurity Enhanced Through Training Evaluation and Raising Awareness"
Ghent : Faculty of veterinary medicine., 31-31.

Maletić J, Pajić M, Đurđević B, Kureljušić B. Biosecurity measures on the broilers and breeders' farms in Serbia. in COST action CA20103 "Biosecurity Enhanced Through Training Evaluation and Raising Awareness". 2023;:31-31..

Maletić, Jelena, Pajić, Marko, Đurđević, Biljana, Kureljušić, Branislav, "Biosecurity measures on the broilers and breeders' farms in Serbia" in COST action CA20103 "Biosecurity Enhanced Through Training Evaluation and Raising Awareness" (2023):31-31.

TY  - JOUR
AU  - Maksimović Zorić, Jelena
AU  - Veljović, Ljubiša
AU  - Radosavljević, Vladimir
AU  - Glišić, Dimitrije
AU  - Kureljušić, Jasna
AU  - Maletić, Jelena
AU  - Savić, Božidar
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/535
AB  - Swine influenza A viruses (swIAVs) are characterised by high mutation rates and zoonotic and pandemicpotential. In order to draw conclusions about virulence in swine and pathogenicity to humans, we examined the existenceof molecular markers and accessory proteins, cross-reactivity with vaccine strains, and resistance to antiviral drugs in five strainsof H1N1 swIAVs. Material and Methods: Amino acid (AA) sequences of five previously genetically characterised swIAVs wereanalysed in MEGA 7.0 software and the Influenza Research Database. Results: Amino acid analysis revealed three virus strainswith 590S/591R polymorphism and T271A substitution within basic polymerase 2 (PB2) AA chains, which cause enhanced virusreplication in mammalian cells. The other two strains possessed D701N and R251K substitutions within PB2 and synthesised PB1-F2protein, which are the factors of increased polymerase activity and virulence in swine. All strains synthesised PB1-N40, PA-N155,PA-N182, and PA-X proteins responsible for enhanced replication in mammalian cells and downregulation of the immune responseof the host. Mutations detected within haemagglutinin antigenic sites imply the antigenic drift of the five analysed viruses in relationto the vaccine strains. All viruses show susceptibility to neuraminidase inhibitors and baloxavir marboxil, which is important insituations of incidental human infections. Conclusion: The detection of virulence markers and accessory proteins in the analysedviruses suggests their higher propensity for replication in mammalian cells, increased virulence, and potential for transmission tohumans, and implies compromised efficacy of influenza vaccines.
PB  - Warsaw : Walter de Gruyter
T2  - Journal of Veterinary Research
T1  - Protein sequence featuresof H1N1 swine influenza A virusesdetected on commercial swine farms in Serbia
EP  - 154
IS  - 2
SP  - 147
VL  - 67
DO  - 10.2478/jvetres-2023-0034
ER  - 

@article{
author = "Maksimović Zorić, Jelena and Veljović, Ljubiša and Radosavljević, Vladimir and Glišić, Dimitrije and Kureljušić, Jasna and Maletić, Jelena and Savić, Božidar",
year = "2023",
abstract = "Swine influenza A viruses (swIAVs) are characterised by high mutation rates and zoonotic and pandemicpotential. In order to draw conclusions about virulence in swine and pathogenicity to humans, we examined the existenceof molecular markers and accessory proteins, cross-reactivity with vaccine strains, and resistance to antiviral drugs in five strainsof H1N1 swIAVs. Material and Methods: Amino acid (AA) sequences of five previously genetically characterised swIAVs wereanalysed in MEGA 7.0 software and the Influenza Research Database. Results: Amino acid analysis revealed three virus strainswith 590S/591R polymorphism and T271A substitution within basic polymerase 2 (PB2) AA chains, which cause enhanced virusreplication in mammalian cells. The other two strains possessed D701N and R251K substitutions within PB2 and synthesised PB1-F2protein, which are the factors of increased polymerase activity and virulence in swine. All strains synthesised PB1-N40, PA-N155,PA-N182, and PA-X proteins responsible for enhanced replication in mammalian cells and downregulation of the immune responseof the host. Mutations detected within haemagglutinin antigenic sites imply the antigenic drift of the five analysed viruses in relationto the vaccine strains. All viruses show susceptibility to neuraminidase inhibitors and baloxavir marboxil, which is important insituations of incidental human infections. Conclusion: The detection of virulence markers and accessory proteins in the analysedviruses suggests their higher propensity for replication in mammalian cells, increased virulence, and potential for transmission tohumans, and implies compromised efficacy of influenza vaccines.",
publisher = "Warsaw : Walter de Gruyter",
journal = "Journal of Veterinary Research",
title = "Protein sequence featuresof H1N1 swine influenza A virusesdetected on commercial swine farms in Serbia",
pages = "154-147",
number = "2",
volume = "67",
doi = "10.2478/jvetres-2023-0034"
}

Maksimović Zorić, J., Veljović, L., Radosavljević, V., Glišić, D., Kureljušić, J., Maletić, J.,& Savić, B.. (2023). Protein sequence featuresof H1N1 swine influenza A virusesdetected on commercial swine farms in Serbia. in Journal of Veterinary Research
Warsaw : Walter de Gruyter., 67(2), 147-154.
https://doi.org/10.2478/jvetres-2023-0034

Maksimović Zorić J, Veljović L, Radosavljević V, Glišić D, Kureljušić J, Maletić J, Savić B. Protein sequence featuresof H1N1 swine influenza A virusesdetected on commercial swine farms in Serbia. in Journal of Veterinary Research. 2023;67(2):147-154.
doi:10.2478/jvetres-2023-0034 .

Maksimović Zorić, Jelena, Veljović, Ljubiša, Radosavljević, Vladimir, Glišić, Dimitrije, Kureljušić, Jasna, Maletić, Jelena, Savić, Božidar, "Protein sequence featuresof H1N1 swine influenza A virusesdetected on commercial swine farms in Serbia" in Journal of Veterinary Research, 67, no. 2 (2023):147-154,
https://doi.org/10.2478/jvetres-2023-0034 . .

TY  - CONF
AU  - Pajić, Marko
AU  - Knežević, Slobodan
AU  - Spiridonović, Sava
AU  - Đurđević, Biljana
AU  - Maletić, Jelena
AU  - Đukić Stojčić, Mirjana
AU  - Perić, Lidija
PY  - 2023
UR  - isasevent.com
UR  - https://reponivs.nivs.rs/handle/123456789/834
AB  - Coccidiosis is one of the most common parasitic infections, which causes large economic losses in broiler farms worldwide. In most cases, it occurs in the form of a subclinical infection and reduction of production performance (body weight, feed conversion ratio) is present. The appearance of this form of the disease most often occurs due to the development of coccidia resistance to coccidiostats or if coccidiostats are not applied in a planned manner. The aim of this study was to examine the presence of subclinical coccidiosis in broiler chicken farms using the McMaster flotation technique. The study was conducted on 15 broiler farms of different capacities (5,000-30,000). A pool sample of faeces was collected from each farm when the chickens were 3-6 weeks of age. Pooled faecal droppings were collected near the feed and watering system, starting from one corner of a unit and following a „W“ pathway across the unit. Using the McMaster technique, the presence of coccidia oocysts was detected on 13 farms (86.7%). The number of oocysts ranged from 3.5 x 104 - 8.3 x 105. The size of the oocysts ranged from 17.1 to 28.6 µm. Data on the size of oocysts indicate the suspicion that a mixed infection with different species of coccidia was present in the majority of positive flocks. In order to prevent the occurrence of subclinical coccidiosis on these farms, it is necessary to improve biosecurity measures, implement high-quality disinfection and rotate coccidiostats.
PB  - Novi Sad : Faculty of agriculture
C3  - Book of Abstracts, International symposium on animal sciences, Novi Sad, Serbia
T1  - Diagnosis of subclinical coccidiosis on broiler farms using the mcmaster technique
EP  - 26
SP  - 26
ER  - 

@conference{
author = "Pajić, Marko and Knežević, Slobodan and Spiridonović, Sava and Đurđević, Biljana and Maletić, Jelena and Đukić Stojčić, Mirjana and Perić, Lidija",
year = "2023",
abstract = "Coccidiosis is one of the most common parasitic infections, which causes large economic losses in broiler farms worldwide. In most cases, it occurs in the form of a subclinical infection and reduction of production performance (body weight, feed conversion ratio) is present. The appearance of this form of the disease most often occurs due to the development of coccidia resistance to coccidiostats or if coccidiostats are not applied in a planned manner. The aim of this study was to examine the presence of subclinical coccidiosis in broiler chicken farms using the McMaster flotation technique. The study was conducted on 15 broiler farms of different capacities (5,000-30,000). A pool sample of faeces was collected from each farm when the chickens were 3-6 weeks of age. Pooled faecal droppings were collected near the feed and watering system, starting from one corner of a unit and following a „W“ pathway across the unit. Using the McMaster technique, the presence of coccidia oocysts was detected on 13 farms (86.7%). The number of oocysts ranged from 3.5 x 104 - 8.3 x 105. The size of the oocysts ranged from 17.1 to 28.6 µm. Data on the size of oocysts indicate the suspicion that a mixed infection with different species of coccidia was present in the majority of positive flocks. In order to prevent the occurrence of subclinical coccidiosis on these farms, it is necessary to improve biosecurity measures, implement high-quality disinfection and rotate coccidiostats.",
publisher = "Novi Sad : Faculty of agriculture",
journal = "Book of Abstracts, International symposium on animal sciences, Novi Sad, Serbia",
title = "Diagnosis of subclinical coccidiosis on broiler farms using the mcmaster technique",
pages = "26-26"
}

Pajić, M., Knežević, S., Spiridonović, S., Đurđević, B., Maletić, J., Đukić Stojčić, M.,& Perić, L.. (2023). Diagnosis of subclinical coccidiosis on broiler farms using the mcmaster technique. in Book of Abstracts, International symposium on animal sciences, Novi Sad, Serbia
Novi Sad : Faculty of agriculture., 26-26.

Pajić M, Knežević S, Spiridonović S, Đurđević B, Maletić J, Đukić Stojčić M, Perić L. Diagnosis of subclinical coccidiosis on broiler farms using the mcmaster technique. in Book of Abstracts, International symposium on animal sciences, Novi Sad, Serbia. 2023;:26-26..

Pajić, Marko, Knežević, Slobodan, Spiridonović, Sava, Đurđević, Biljana, Maletić, Jelena, Đukić Stojčić, Mirjana, Perić, Lidija, "Diagnosis of subclinical coccidiosis on broiler farms using the mcmaster technique" in Book of Abstracts, International symposium on animal sciences, Novi Sad, Serbia (2023):26-26.

TY  - JOUR
AU  - Maletić, Jelena
AU  - Kureljušić, Jasna
AU  - Katanić, Nenad
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/808
AB  - As a preventive measure, biosecurity is the first line of defense against many pathogens.
Applied biosecurity measures can reduce the prevalence of Campylobacter infection in commercial
broiler populations. A systematic evaluation, encompassing at least annual monitoring
of applied biosecurity measures and on‑farm prevalence of Campylobacter infection is
highly recommended.
This study was performed on three broiler farms with the aim to assess the effectiveness
of the biosecurity measures applied. Broiler farms included in the study previously had
problems with Campylobacter infections, and therefore, after the intervention through a
risk‑based scoring system and bacteriological testing of samples from the farm and the corresponding
carcasses in the slaughterhouse, several biosecurity measures were implemented.
Obtained results showed that after the intervention, farms increased their external biosecurity
by 16.34%, internal biosecurity by 22%, and overall biosecurity by 18.34%. The major
interventions concerned the removal of manure and carcasses, all improved measures taken
for feed and drinking water, and measures in the subcategory of cleaning and disinfection
protocols carried out between two production cycles. After the improvements, during the
screening process on the farms, Campylobacter was not isolated from pooled fecal samples
in any of the broiler houses. This indicates that at least six houses (two houses per farm)
were Campylobacter‑negative at broiler slaughter age. In pooled neck skin samples originating
from studied farms, Campylobacter was not isolated after the improved measures were
implemented.
The results showed that the assessment of biosecurity protocols on broiler farms is a useful
tool, and Campylobacter can serve as a biomarker for the efficiency of the implemented
biosecurity protocols.
PB  - Belgrade : Institute of Meat Hygiene and Technology
T2  - Meat Technology — Special Issue
T1  - Enhanced biosecurity measures may contribute to the reduction of Campylobacter incidence in slaughterhouses
EP  - 100
IS  - 2
SP  - 93
VL  - 64
DO  - 10.18485/meattech.2023.64.2.16
ER  - 

@article{
author = "Maletić, Jelena and Kureljušić, Jasna and Katanić, Nenad",
year = "2023",
abstract = "As a preventive measure, biosecurity is the first line of defense against many pathogens.
Applied biosecurity measures can reduce the prevalence of Campylobacter infection in commercial
broiler populations. A systematic evaluation, encompassing at least annual monitoring
of applied biosecurity measures and on‑farm prevalence of Campylobacter infection is
highly recommended.
This study was performed on three broiler farms with the aim to assess the effectiveness
of the biosecurity measures applied. Broiler farms included in the study previously had
problems with Campylobacter infections, and therefore, after the intervention through a
risk‑based scoring system and bacteriological testing of samples from the farm and the corresponding
carcasses in the slaughterhouse, several biosecurity measures were implemented.
Obtained results showed that after the intervention, farms increased their external biosecurity
by 16.34%, internal biosecurity by 22%, and overall biosecurity by 18.34%. The major
interventions concerned the removal of manure and carcasses, all improved measures taken
for feed and drinking water, and measures in the subcategory of cleaning and disinfection
protocols carried out between two production cycles. After the improvements, during the
screening process on the farms, Campylobacter was not isolated from pooled fecal samples
in any of the broiler houses. This indicates that at least six houses (two houses per farm)
were Campylobacter‑negative at broiler slaughter age. In pooled neck skin samples originating
from studied farms, Campylobacter was not isolated after the improved measures were
implemented.
The results showed that the assessment of biosecurity protocols on broiler farms is a useful
tool, and Campylobacter can serve as a biomarker for the efficiency of the implemented
biosecurity protocols.",
publisher = "Belgrade : Institute of Meat Hygiene and Technology",
journal = "Meat Technology — Special Issue",
title = "Enhanced biosecurity measures may contribute to the reduction of Campylobacter incidence in slaughterhouses",
pages = "100-93",
number = "2",
volume = "64",
doi = "10.18485/meattech.2023.64.2.16"
}

Maletić, J., Kureljušić, J.,& Katanić, N.. (2023). Enhanced biosecurity measures may contribute to the reduction of Campylobacter incidence in slaughterhouses. in Meat Technology — Special Issue
Belgrade : Institute of Meat Hygiene and Technology., 64(2), 93-100.
https://doi.org/10.18485/meattech.2023.64.2.16

Maletić J, Kureljušić J, Katanić N. Enhanced biosecurity measures may contribute to the reduction of Campylobacter incidence in slaughterhouses. in Meat Technology — Special Issue. 2023;64(2):93-100.
doi:10.18485/meattech.2023.64.2.16 .

Maletić, Jelena, Kureljušić, Jasna, Katanić, Nenad, "Enhanced biosecurity measures may contribute to the reduction of Campylobacter incidence in slaughterhouses" in Meat Technology — Special Issue, 64, no. 2 (2023):93-100,
https://doi.org/10.18485/meattech.2023.64.2.16 . .

TY  - JOUR
AU  - Veljović, Ljubiša
AU  - Maksimović Zorić, Jelena
AU  - Glišić, Dimitrije
AU  - Nišavić, Jakov
AU  - Maletić, Jelena
AU  - Milićević, Vesna
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/906
AB  - Schmallenberg disease is an infectious disease of cattle, sheep, and goats of viral
aetiology caused by the Schmallenberg virus that belongs to the family Bunyaviridae and
the genus Ortobunyavirus. Schmallenberg disease is a vector-borne disease transmitted by
midges from the genus Culicoides but also by other hematophagous insects. The disease
has a seasonal character and most often occurs in the warm months, from late spring
to autumn. In this study, we investigated the seroprevalence of Schmallenberg disease
in sheep in the epizootic area of Belgrade in the period from 2017 to 2022. A total of
600 sheep serums from the serum bank were tested, 100 serum samples from each year.
Sera were tested by commercial ELISA test for the detection of specific antibodies
against the Schmallenberg virus. The results showed an average annual seroprevalence
of 24.5% in sheep in the Belgrade area in these six years with an increasing trend
predicted for the next years.
PB  - Belgrade : Faculty of Veterinary Medicine, University of Belgrade
T2  - Acta Veterinaria-Beograd
T1  - Seroprevalence of Schmallenberg virus in sheep in Belgrade epizootic area
EP  - 510
IS  - 4
SP  - 502
VL  - 73
DO  - 10.2478/acve-2023-0038
ER  - 

@article{
author = "Veljović, Ljubiša and Maksimović Zorić, Jelena and Glišić, Dimitrije and Nišavić, Jakov and Maletić, Jelena and Milićević, Vesna",
year = "2023",
abstract = "Schmallenberg disease is an infectious disease of cattle, sheep, and goats of viral
aetiology caused by the Schmallenberg virus that belongs to the family Bunyaviridae and
the genus Ortobunyavirus. Schmallenberg disease is a vector-borne disease transmitted by
midges from the genus Culicoides but also by other hematophagous insects. The disease
has a seasonal character and most often occurs in the warm months, from late spring
to autumn. In this study, we investigated the seroprevalence of Schmallenberg disease
in sheep in the epizootic area of Belgrade in the period from 2017 to 2022. A total of
600 sheep serums from the serum bank were tested, 100 serum samples from each year.
Sera were tested by commercial ELISA test for the detection of specific antibodies
against the Schmallenberg virus. The results showed an average annual seroprevalence
of 24.5% in sheep in the Belgrade area in these six years with an increasing trend
predicted for the next years.",
publisher = "Belgrade : Faculty of Veterinary Medicine, University of Belgrade",
journal = "Acta Veterinaria-Beograd",
title = "Seroprevalence of Schmallenberg virus in sheep in Belgrade epizootic area",
pages = "510-502",
number = "4",
volume = "73",
doi = "10.2478/acve-2023-0038"
}

Veljović, L., Maksimović Zorić, J., Glišić, D., Nišavić, J., Maletić, J.,& Milićević, V.. (2023). Seroprevalence of Schmallenberg virus in sheep in Belgrade epizootic area. in Acta Veterinaria-Beograd
Belgrade : Faculty of Veterinary Medicine, University of Belgrade., 73(4), 502-510.
https://doi.org/10.2478/acve-2023-0038

Veljović L, Maksimović Zorić J, Glišić D, Nišavić J, Maletić J, Milićević V. Seroprevalence of Schmallenberg virus in sheep in Belgrade epizootic area. in Acta Veterinaria-Beograd. 2023;73(4):502-510.
doi:10.2478/acve-2023-0038 .

Veljović, Ljubiša, Maksimović Zorić, Jelena, Glišić, Dimitrije, Nišavić, Jakov, Maletić, Jelena, Milićević, Vesna, "Seroprevalence of Schmallenberg virus in sheep in Belgrade epizootic area" in Acta Veterinaria-Beograd, 73, no. 4 (2023):502-510,
https://doi.org/10.2478/acve-2023-0038 . .

TY  - JOUR
AU  - Glišić, Dimitrije
AU  - Veljović, Ljubiša
AU  - Milovanović, Bojan
AU  - Ninković, Milan
AU  - Maletić, Jelena
AU  - Kureljušić, Branislav
AU  - Milićević, Vesna
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/849
AB  - Monitoring the health of swine herds is essential to ensure good manufacturing
practices. Traditionally, active and passive surveillance on farms involved invasive
sampling methods, where specific animals were selected, restrained, and sampled.
However, with the increasing intensity of swine production, alternative methods for
effective herd surveillance became necessary.
Non-invasive sampling provides a convenient and cost-effective approach to monitor
the entire herd without compromising animal welfare, while still obtaining suitable
samples for testing. Oral fluids have been widely used in both human and livestock
health surveillance for various viral pathogens, including significant diseases. Nasal
wipes (NW) utilize different cloth materials soaked in phosphate-buffered saline
(PBS) or tissue culture medium with antibiotics and antimycotics to sample for swine
influenza virus (SIV). Udder skin wipes (USW) offer an alternative method to assess
the health status of piglets in a litter. During routine procedures such as tail docking
and castration, a mixture of blood and serum can be collected, known as process fluids
(PF), which has proven successful in monitoring herds for the presence of porcine
reproductive and respiratory syndrome virus (PRRSV). Furthermore, air sampling has emerged as a novel technique to detect pathogens in various farming systems and
animal species. This method offers the advantage of obtaining diagnostic samples
without direct animal contact.
By employing these non-invasive sampling methods, swine producers can implement
effective surveillance strategies while maintaining animal welfare standards and
obtaining reliable diagnostic information.
PB  - Beograd : Fakultet veterinarske medicine, Univerzitet u Beogradu
PB  - Beograd : Veterinarska komora Srbije
T2  - Veterinarski glasnik
T1  - Overview of non-invasive sampling methods used in intensive swine farming
EP  - 108
IS  - 2
SP  - 97
VL  - 77
DO  - 10.2298/VETGL230614004G
ER  - 

@article{
author = "Glišić, Dimitrije and Veljović, Ljubiša and Milovanović, Bojan and Ninković, Milan and Maletić, Jelena and Kureljušić, Branislav and Milićević, Vesna",
year = "2023",
abstract = "Monitoring the health of swine herds is essential to ensure good manufacturing
practices. Traditionally, active and passive surveillance on farms involved invasive
sampling methods, where specific animals were selected, restrained, and sampled.
However, with the increasing intensity of swine production, alternative methods for
effective herd surveillance became necessary.
Non-invasive sampling provides a convenient and cost-effective approach to monitor
the entire herd without compromising animal welfare, while still obtaining suitable
samples for testing. Oral fluids have been widely used in both human and livestock
health surveillance for various viral pathogens, including significant diseases. Nasal
wipes (NW) utilize different cloth materials soaked in phosphate-buffered saline
(PBS) or tissue culture medium with antibiotics and antimycotics to sample for swine
influenza virus (SIV). Udder skin wipes (USW) offer an alternative method to assess
the health status of piglets in a litter. During routine procedures such as tail docking
and castration, a mixture of blood and serum can be collected, known as process fluids
(PF), which has proven successful in monitoring herds for the presence of porcine
reproductive and respiratory syndrome virus (PRRSV). Furthermore, air sampling has emerged as a novel technique to detect pathogens in various farming systems and
animal species. This method offers the advantage of obtaining diagnostic samples
without direct animal contact.
By employing these non-invasive sampling methods, swine producers can implement
effective surveillance strategies while maintaining animal welfare standards and
obtaining reliable diagnostic information.",
publisher = "Beograd : Fakultet veterinarske medicine, Univerzitet u Beogradu, Beograd : Veterinarska komora Srbije",
journal = "Veterinarski glasnik",
title = "Overview of non-invasive sampling methods used in intensive swine farming",
pages = "108-97",
number = "2",
volume = "77",
doi = "10.2298/VETGL230614004G"
}

Glišić, D., Veljović, L., Milovanović, B., Ninković, M., Maletić, J., Kureljušić, B.,& Milićević, V.. (2023). Overview of non-invasive sampling methods used in intensive swine farming. in Veterinarski glasnik
Beograd : Fakultet veterinarske medicine, Univerzitet u Beogradu., 77(2), 97-108.
https://doi.org/10.2298/VETGL230614004G

Glišić D, Veljović L, Milovanović B, Ninković M, Maletić J, Kureljušić B, Milićević V. Overview of non-invasive sampling methods used in intensive swine farming. in Veterinarski glasnik. 2023;77(2):97-108.
doi:10.2298/VETGL230614004G .

Glišić, Dimitrije, Veljović, Ljubiša, Milovanović, Bojan, Ninković, Milan, Maletić, Jelena, Kureljušić, Branislav, Milićević, Vesna, "Overview of non-invasive sampling methods used in intensive swine farming" in Veterinarski glasnik, 77, no. 2 (2023):97-108,
https://doi.org/10.2298/VETGL230614004G . .

TY  - JOUR
AU  - Milićević, Vesna
AU  - Glišić, Dimitrije
AU  - Veljović, Ljubiša
AU  - Maletić, Jelena
AU  - Kureljušić, Branislav
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/835
AB  - Rabies is a viral disease of the central nervous system of all warm-blooded animals
and one of the oldest and most important zoonoses. In the Republic of Serbia, rabies is controlled by compulsory vaccination of dogs and cats and oral vaccination of
wild carnivores, which has been implemented since 2010. In the period 2009–2018,
367 rabies cases were recorded. The last rabies case in Serbia was recorded in 2018
in a red fox (Vulpes vulpes), while the last case in dogs was diagnosed in 2011. This
study aimed to perform a retrospective phylogenetic analysis of G and N genes of
the rabies virus from Serbia from 2009 to 2018 to understand sources and pathways
of infection better and to enable molecular virus tracing in the future based on the
association of rabies virus genetic lineages with the geographic distribution. For the
phylogenetic analysis of the rabies virus, 14 historically positive brain samples of red
foxes from 2009 to 2018, collected in central Serbia, were used. All field strains from
Serbia were identified as classic rabies virus and grouped within the Cosmopolitan lineage. Phylogenetic analysis of N gene sequences revealed that 13 rabies virus strains
(92.9%) from Serbia belonged to the EE sub-lineage of isolates, while one virus (7.1%)
ON988027 from 2011 clustered together with isolates from the WE sub-lineage.
PB  - Wiley
T2  - Zoonoses and Public Health
T1  - Retrospective phylogenetic analysis of rabies virus G and N genes from Serbia
EP  - 704
IS  - 8
SP  - 699
VL  - 70
DO  - 10.1111/zph.13061
ER  - 

@article{
author = "Milićević, Vesna and Glišić, Dimitrije and Veljović, Ljubiša and Maletić, Jelena and Kureljušić, Branislav",
year = "2023",
abstract = "Rabies is a viral disease of the central nervous system of all warm-blooded animals
and one of the oldest and most important zoonoses. In the Republic of Serbia, rabies is controlled by compulsory vaccination of dogs and cats and oral vaccination of
wild carnivores, which has been implemented since 2010. In the period 2009–2018,
367 rabies cases were recorded. The last rabies case in Serbia was recorded in 2018
in a red fox (Vulpes vulpes), while the last case in dogs was diagnosed in 2011. This
study aimed to perform a retrospective phylogenetic analysis of G and N genes of
the rabies virus from Serbia from 2009 to 2018 to understand sources and pathways
of infection better and to enable molecular virus tracing in the future based on the
association of rabies virus genetic lineages with the geographic distribution. For the
phylogenetic analysis of the rabies virus, 14 historically positive brain samples of red
foxes from 2009 to 2018, collected in central Serbia, were used. All field strains from
Serbia were identified as classic rabies virus and grouped within the Cosmopolitan lineage. Phylogenetic analysis of N gene sequences revealed that 13 rabies virus strains
(92.9%) from Serbia belonged to the EE sub-lineage of isolates, while one virus (7.1%)
ON988027 from 2011 clustered together with isolates from the WE sub-lineage.",
publisher = "Wiley",
journal = "Zoonoses and Public Health",
title = "Retrospective phylogenetic analysis of rabies virus G and N genes from Serbia",
pages = "704-699",
number = "8",
volume = "70",
doi = "10.1111/zph.13061"
}

Milićević, V., Glišić, D., Veljović, L., Maletić, J.,& Kureljušić, B.. (2023). Retrospective phylogenetic analysis of rabies virus G and N genes from Serbia. in Zoonoses and Public Health
Wiley., 70(8), 699-704.
https://doi.org/10.1111/zph.13061

Milićević V, Glišić D, Veljović L, Maletić J, Kureljušić B. Retrospective phylogenetic analysis of rabies virus G and N genes from Serbia. in Zoonoses and Public Health. 2023;70(8):699-704.
doi:10.1111/zph.13061 .

Milićević, Vesna, Glišić, Dimitrije, Veljović, Ljubiša, Maletić, Jelena, Kureljušić, Branislav, "Retrospective phylogenetic analysis of rabies virus G and N genes from Serbia" in Zoonoses and Public Health, 70, no. 8 (2023):699-704,
https://doi.org/10.1111/zph.13061 . .

TY  - JOUR
AU  - Maletić, Jelena
AU  - Spalević, Ljiljana
AU  - Milićević, Vesna
AU  - Glišić, Dimitrije
AU  - Kureljušić, Branislav
AU  - Kureljušić, Jasna
AU  - Maletić, Milan
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/832
AB  - The implementation of biosecurity measures as the first line of defense is an essential prerequisite for preventing the occurrence and spread of infectious agents in flocks. Assessment of biosecurity measures on the farms was carried out during farm visits based on the application of an appropriate questionnaire whereby the farmer answered a number of questions regarding the implemented biosecurity measures. The study was performed via Biocheck.UGent online survey on 16 broiler farms in the region of the city of Belgrade. The capacity of farms was 25,000-100,000 broilers, placed in 2-4 houses, depending on the farm. Farm visits were made by the authors to confirm the correct answers to the online survey. Results showed that external biosecurity scores ranged from 57% to 93%, averaging 83.6%. Internal biosecurity score ranged from 48% to 98%, with an average score of 85.7%. The total score for the biosecurity assessment ranged from 56% to 93%, with an average score of 84.3%. The scores for subcategories varied between the farms. Removing manure and carcasses, a subcategory within the category of external biosecurity, had the lowest mean score (farms 9 and 10 had a score of 12%). Relatively low scores were also obtained for the number of steps of broilers depopulation (farm 9 had a score of 44%) and for the location of the farm (farms 2 and 3 had a score of 44%). Concerning internal biosecurity, the lowest score was obtained for the subcategory material and measures that they apply between compartments (farm 10 had a score of 29%). These findings should be useful for decision-makers and flock veterinarians and farmers to set feasible targets and sustainable biosecurity programs to improve biosecurity, the health status of the flock, and farm profitability.
AB  - Sprovođenje biosigurnosnih mera, kao prve linije odbrane, suštinski je preduslov za sprečavanje pojave i širenja infektivnih oboljenja kod živine. Procena biosigurnosnih mera na farmama izvršena je primenom odgovarajućeg upitnika, gde je farmer odgovorio na brojna pitanja u vezi sa sprovedenim biosigurnosnim merama. Istraživanje je sprovedeno putem onlajn ankete Biocheck.Ugent na 16 farmi brojlera u regionu grada Beograda. Kapacitet farmi je bio 25000-100000 brojlera, smeštenih u 2 - 4 objekta, u zavisnosti od farme. Rezultati su pokazali da se eksterna biosigurnost kretala od 57 % do 93 %, sa prosečnom ocenom od 83.6 %. Rezultat interne biosigurnosti se kretao od 48 % do 98 %, sa prosečnom ocenom od 85.7 %. Krajnji rezultat procene biosigurnosti kretao se od 56 % do 93 %, sa prosečnom ocenom od 84.3%. Rezultati za potkategorije su varirali između farmi. Uklanjanje stajnjaka i leševa, potkategorija u okviru kategorije eksterne biobezbednosti, imala je najnižu srednju ocenu (farme 9 i 10 su imale ocenu od 12 %). Niže ocene takođe su dobijene za potkategorije broj koraka depopulacije brojlera (farma 9 je imala ocenu 44 %) i za lokaciju farme (farme 2 i 3 su imale ocenu od 44 %). Što se tiče interne biosigurnosti, najniža ocena je dobijena za potkategoriju materijal i mere koje se primenjuju između odeljaka (farma 10 imala je ocenu od 29 %). Ovi nalazi trebalo bi da budu korisni donosiocima odluka i veterinarima i farmerima u postavljanju izvodljivih ciljeva i održivih biosigurnosnih programa, za poboljšanje biobezbednosti, zdravstvenog statusa jata i profitabilnosti farme.
PB  - Beograd : Fakultet veterinarske medicine, Univerzitet u Beogradu
T2  - Veterinarski glasnik
T1  - Assessment of biosecurity measures implemented on the broiler farms in the region of Belgrade city
T1  - Ocena biosigurnosnih mera implementiranih na farmama brojlera na beogradskom području
EP  - 136
IS  - 2
SP  - 125
VL  - 77
DO  - 10.2298/VETGL230403003M
ER  - 

@article{
author = "Maletić, Jelena and Spalević, Ljiljana and Milićević, Vesna and Glišić, Dimitrije and Kureljušić, Branislav and Kureljušić, Jasna and Maletić, Milan",
year = "2023",
abstract = "The implementation of biosecurity measures as the first line of defense is an essential prerequisite for preventing the occurrence and spread of infectious agents in flocks. Assessment of biosecurity measures on the farms was carried out during farm visits based on the application of an appropriate questionnaire whereby the farmer answered a number of questions regarding the implemented biosecurity measures. The study was performed via Biocheck.UGent online survey on 16 broiler farms in the region of the city of Belgrade. The capacity of farms was 25,000-100,000 broilers, placed in 2-4 houses, depending on the farm. Farm visits were made by the authors to confirm the correct answers to the online survey. Results showed that external biosecurity scores ranged from 57% to 93%, averaging 83.6%. Internal biosecurity score ranged from 48% to 98%, with an average score of 85.7%. The total score for the biosecurity assessment ranged from 56% to 93%, with an average score of 84.3%. The scores for subcategories varied between the farms. Removing manure and carcasses, a subcategory within the category of external biosecurity, had the lowest mean score (farms 9 and 10 had a score of 12%). Relatively low scores were also obtained for the number of steps of broilers depopulation (farm 9 had a score of 44%) and for the location of the farm (farms 2 and 3 had a score of 44%). Concerning internal biosecurity, the lowest score was obtained for the subcategory material and measures that they apply between compartments (farm 10 had a score of 29%). These findings should be useful for decision-makers and flock veterinarians and farmers to set feasible targets and sustainable biosecurity programs to improve biosecurity, the health status of the flock, and farm profitability., Sprovođenje biosigurnosnih mera, kao prve linije odbrane, suštinski je preduslov za sprečavanje pojave i širenja infektivnih oboljenja kod živine. Procena biosigurnosnih mera na farmama izvršena je primenom odgovarajućeg upitnika, gde je farmer odgovorio na brojna pitanja u vezi sa sprovedenim biosigurnosnim merama. Istraživanje je sprovedeno putem onlajn ankete Biocheck.Ugent na 16 farmi brojlera u regionu grada Beograda. Kapacitet farmi je bio 25000-100000 brojlera, smeštenih u 2 - 4 objekta, u zavisnosti od farme. Rezultati su pokazali da se eksterna biosigurnost kretala od 57 % do 93 %, sa prosečnom ocenom od 83.6 %. Rezultat interne biosigurnosti se kretao od 48 % do 98 %, sa prosečnom ocenom od 85.7 %. Krajnji rezultat procene biosigurnosti kretao se od 56 % do 93 %, sa prosečnom ocenom od 84.3%. Rezultati za potkategorije su varirali između farmi. Uklanjanje stajnjaka i leševa, potkategorija u okviru kategorije eksterne biobezbednosti, imala je najnižu srednju ocenu (farme 9 i 10 su imale ocenu od 12 %). Niže ocene takođe su dobijene za potkategorije broj koraka depopulacije brojlera (farma 9 je imala ocenu 44 %) i za lokaciju farme (farme 2 i 3 su imale ocenu od 44 %). Što se tiče interne biosigurnosti, najniža ocena je dobijena za potkategoriju materijal i mere koje se primenjuju između odeljaka (farma 10 imala je ocenu od 29 %). Ovi nalazi trebalo bi da budu korisni donosiocima odluka i veterinarima i farmerima u postavljanju izvodljivih ciljeva i održivih biosigurnosnih programa, za poboljšanje biobezbednosti, zdravstvenog statusa jata i profitabilnosti farme.",
publisher = "Beograd : Fakultet veterinarske medicine, Univerzitet u Beogradu",
journal = "Veterinarski glasnik",
title = "Assessment of biosecurity measures implemented on the broiler farms in the region of Belgrade city, Ocena biosigurnosnih mera implementiranih na farmama brojlera na beogradskom području",
pages = "136-125",
number = "2",
volume = "77",
doi = "10.2298/VETGL230403003M"
}

Maletić, J., Spalević, L., Milićević, V., Glišić, D., Kureljušić, B., Kureljušić, J.,& Maletić, M.. (2023). Assessment of biosecurity measures implemented on the broiler farms in the region of Belgrade city. in Veterinarski glasnik
Beograd : Fakultet veterinarske medicine, Univerzitet u Beogradu., 77(2), 125-136.
https://doi.org/10.2298/VETGL230403003M

Maletić J, Spalević L, Milićević V, Glišić D, Kureljušić B, Kureljušić J, Maletić M. Assessment of biosecurity measures implemented on the broiler farms in the region of Belgrade city. in Veterinarski glasnik. 2023;77(2):125-136.
doi:10.2298/VETGL230403003M .

Maletić, Jelena, Spalević, Ljiljana, Milićević, Vesna, Glišić, Dimitrije, Kureljušić, Branislav, Kureljušić, Jasna, Maletić, Milan, "Assessment of biosecurity measures implemented on the broiler farms in the region of Belgrade city" in Veterinarski glasnik, 77, no. 2 (2023):125-136,
https://doi.org/10.2298/VETGL230403003M . .

TY  - CONF
AU  - Milićević, Vesna
AU  - Glišić, Dimitrije
AU  - Milovanović, Bojan
AU  - Veljović, Ljubiša
AU  - Maletić, Jelena
AU  - Kureljušić, Branislav
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/803
AB  - Non-invasive sampling is increasingly used in domestic and wild animals. Traditional sampling methods are labour, time-consuming, and require trained personnel. Oral fluid is an alternative sample that can replace nasal swabs. Air sampling with special device and testing represents the newest method of non-invasive sampling, which is particularly useful and validated when testing pigs. Udder skin sampling is a new sampling approach that provides insight into litters of pigs before weaning, containing their nasal and oral secretions.
PB  - ECPHM
C3  - Proceedings : ESPHM : 14th European symposium of porcine health management
T1  - Porcine viral diseases can be successfully  monitored using non-invasive sampling strategy
EP  - 317
SP  - 317
ER  - 

@conference{
author = "Milićević, Vesna and Glišić, Dimitrije and Milovanović, Bojan and Veljović, Ljubiša and Maletić, Jelena and Kureljušić, Branislav",
year = "2023",
abstract = "Non-invasive sampling is increasingly used in domestic and wild animals. Traditional sampling methods are labour, time-consuming, and require trained personnel. Oral fluid is an alternative sample that can replace nasal swabs. Air sampling with special device and testing represents the newest method of non-invasive sampling, which is particularly useful and validated when testing pigs. Udder skin sampling is a new sampling approach that provides insight into litters of pigs before weaning, containing their nasal and oral secretions.",
publisher = "ECPHM",
journal = "Proceedings : ESPHM : 14th European symposium of porcine health management",
title = "Porcine viral diseases can be successfully  monitored using non-invasive sampling strategy",
pages = "317-317"
}

Milićević, V., Glišić, D., Milovanović, B., Veljović, L., Maletić, J.,& Kureljušić, B.. (2023). Porcine viral diseases can be successfully  monitored using non-invasive sampling strategy. in Proceedings : ESPHM : 14th European symposium of porcine health management
ECPHM., 317-317.

Milićević V, Glišić D, Milovanović B, Veljović L, Maletić J, Kureljušić B. Porcine viral diseases can be successfully  monitored using non-invasive sampling strategy. in Proceedings : ESPHM : 14th European symposium of porcine health management. 2023;:317-317..

Milićević, Vesna, Glišić, Dimitrije, Milovanović, Bojan, Veljović, Ljubiša, Maletić, Jelena, Kureljušić, Branislav, "Porcine viral diseases can be successfully  monitored using non-invasive sampling strategy" in Proceedings : ESPHM : 14th European symposium of porcine health management (2023):317-317.

TY  - CONF
AU  - Kureljušić, Branislav
AU  - Milićević, Vesna
AU  - Milovanović, Bojan
AU  - Glišić, Dimitrije
AU  - Maletić, Jelena
AU  - Jezdimirović, Nemanja
AU  - Kureljušić, Jasna
AU  - Prodanov Radulović, Jasna
AU  - Savić, Božidar
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/804
AB  - Biosecurity ls of paramount Importance to prevent the introduction and spread of pathogens in pig farms. Applied biosecurity measures and efficient on-farm management protect pigs from different infectious diseases. African swine fever CASF) is a severe, viral disease of domestic pigs and wild boar. After the occurrence in 2007 in Georgia, the disease gradually spread throughout Europe. The first case of ASF in Serbia was confirmed on July 30, 2019.
PB  - ECPHM
C3  - Proceedings : ESPHM : 14th European symposium of porcine health management
T1  - Assessment of biosecurity measures in the Serbian backyards where the first African swine fever cases were confirmed
EP  - 253
SP  - 253
ER  - 

@conference{
author = "Kureljušić, Branislav and Milićević, Vesna and Milovanović, Bojan and Glišić, Dimitrije and Maletić, Jelena and Jezdimirović, Nemanja and Kureljušić, Jasna and Prodanov Radulović, Jasna and Savić, Božidar",
year = "2023",
abstract = "Biosecurity ls of paramount Importance to prevent the introduction and spread of pathogens in pig farms. Applied biosecurity measures and efficient on-farm management protect pigs from different infectious diseases. African swine fever CASF) is a severe, viral disease of domestic pigs and wild boar. After the occurrence in 2007 in Georgia, the disease gradually spread throughout Europe. The first case of ASF in Serbia was confirmed on July 30, 2019.",
publisher = "ECPHM",
journal = "Proceedings : ESPHM : 14th European symposium of porcine health management",
title = "Assessment of biosecurity measures in the Serbian backyards where the first African swine fever cases were confirmed",
pages = "253-253"
}

Kureljušić, B., Milićević, V., Milovanović, B., Glišić, D., Maletić, J., Jezdimirović, N., Kureljušić, J., Prodanov Radulović, J.,& Savić, B.. (2023). Assessment of biosecurity measures in the Serbian backyards where the first African swine fever cases were confirmed. in Proceedings : ESPHM : 14th European symposium of porcine health management
ECPHM., 253-253.

Kureljušić B, Milićević V, Milovanović B, Glišić D, Maletić J, Jezdimirović N, Kureljušić J, Prodanov Radulović J, Savić B. Assessment of biosecurity measures in the Serbian backyards where the first African swine fever cases were confirmed. in Proceedings : ESPHM : 14th European symposium of porcine health management. 2023;:253-253..

Kureljušić, Branislav, Milićević, Vesna, Milovanović, Bojan, Glišić, Dimitrije, Maletić, Jelena, Jezdimirović, Nemanja, Kureljušić, Jasna, Prodanov Radulović, Jasna, Savić, Božidar, "Assessment of biosecurity measures in the Serbian backyards where the first African swine fever cases were confirmed" in Proceedings : ESPHM : 14th European symposium of porcine health management (2023):253-253.

TY  - CONF
AU  - Maletić, Jelena
AU  - Pajić, M.
AU  - Đurđević, Biljana
AU  - Spalević, Ljiljana
AU  - Milićević, Vesna
AU  - Kureljušić, B.
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/783
AB  - Ekstenzivan način uzgoja živine čini jedan od najvažnijih izvora hrane za domaćinstva u ruralnim sredinama. U urbanim sredinama savremeni način života doveo je do sve većeg interesovanja i potrebe ljudi da kupuju proizvode poreklom od živine koja se uzgaja na ovaj način. 
Ekstenzivan način uzgoja ima svoje prednosti, ali i mane. Mane ovog načina gajenja su povezane sa povećanim rizikom od unosa i širenja patogenih mikroorganizama koji mogu ugroziti zdravlje jedinki koje se tu uzgajaju, i zdravlje ljudi koji brinu o njima i ljudi koji konzumiraju njihove proizvode. U ekstenzivnom uzgoju mnoge zarazne bolesti su uobičajene i mogu se nekontrolisano širiti. Bolesti živine gajene u ekstenzivnom uzgoju mogu biti bakterijske, virusne, gljivične ili parazitske etiologije. „Dvorišna živina" može biti izvor antimikrobne rezistencije i nekoliko važnih zoonotskih patogena, uključujući Salmonella spp., Listeria monocytogenes, Campylobacter spp., i Escherichia coli. 
Prva linija odbrane od patogenih mikroorganizama u svakoj proizvodnji životinja su biosigurnosne mere. 
U slučaju ekstenzivnog uzgoja, neophodno je pre svega razumeti postojeća znanja i navike farmera, a zatim ukazati na mere koje oni u svom domaćinstvu mogu preduzeti kako bi prevenirali pojavu različitih oboljenja. Da bi bile održive, te mere moraju biti fleksibilne i prilagodljive.
PB  - Beograd : Živinarstvo, Centar za informisanje izdavaštvo, inovacije i propagandu
C3  - Živinarstvo : 32. savetovanje živinara
T1  - Ekstezivan uzgoj živine - najčešća oboljenja i izazovi
EP  - 42
IS  - 6/7
SP  - 30
VL  - 57
ER  - 

@conference{
author = "Maletić, Jelena and Pajić, M. and Đurđević, Biljana and Spalević, Ljiljana and Milićević, Vesna and Kureljušić, B.",
year = "2023",
abstract = "Ekstenzivan način uzgoja živine čini jedan od najvažnijih izvora hrane za domaćinstva u ruralnim sredinama. U urbanim sredinama savremeni način života doveo je do sve većeg interesovanja i potrebe ljudi da kupuju proizvode poreklom od živine koja se uzgaja na ovaj način. 
Ekstenzivan način uzgoja ima svoje prednosti, ali i mane. Mane ovog načina gajenja su povezane sa povećanim rizikom od unosa i širenja patogenih mikroorganizama koji mogu ugroziti zdravlje jedinki koje se tu uzgajaju, i zdravlje ljudi koji brinu o njima i ljudi koji konzumiraju njihove proizvode. U ekstenzivnom uzgoju mnoge zarazne bolesti su uobičajene i mogu se nekontrolisano širiti. Bolesti živine gajene u ekstenzivnom uzgoju mogu biti bakterijske, virusne, gljivične ili parazitske etiologije. „Dvorišna živina" može biti izvor antimikrobne rezistencije i nekoliko važnih zoonotskih patogena, uključujući Salmonella spp., Listeria monocytogenes, Campylobacter spp., i Escherichia coli. 
Prva linija odbrane od patogenih mikroorganizama u svakoj proizvodnji životinja su biosigurnosne mere. 
U slučaju ekstenzivnog uzgoja, neophodno je pre svega razumeti postojeća znanja i navike farmera, a zatim ukazati na mere koje oni u svom domaćinstvu mogu preduzeti kako bi prevenirali pojavu različitih oboljenja. Da bi bile održive, te mere moraju biti fleksibilne i prilagodljive.",
publisher = "Beograd : Živinarstvo, Centar za informisanje izdavaštvo, inovacije i propagandu",
journal = "Živinarstvo : 32. savetovanje živinara",
title = "Ekstezivan uzgoj živine - najčešća oboljenja i izazovi",
pages = "42-30",
number = "6/7",
volume = "57"
}

Maletić, J., Pajić, M., Đurđević, B., Spalević, L., Milićević, V.,& Kureljušić, B.. (2023). Ekstezivan uzgoj živine - najčešća oboljenja i izazovi. in Živinarstvo : 32. savetovanje živinara
Beograd : Živinarstvo, Centar za informisanje izdavaštvo, inovacije i propagandu., 57(6/7), 30-42.

Maletić J, Pajić M, Đurđević B, Spalević L, Milićević V, Kureljušić B. Ekstezivan uzgoj živine - najčešća oboljenja i izazovi. in Živinarstvo : 32. savetovanje živinara. 2023;57(6/7):30-42..

Maletić, Jelena, Pajić, M., Đurđević, Biljana, Spalević, Ljiljana, Milićević, Vesna, Kureljušić, B., "Ekstezivan uzgoj živine - najčešća oboljenja i izazovi" in Živinarstvo : 32. savetovanje živinara, 57, no. 6/7 (2023):30-42.

TY  - JOUR
AU  - Milićević, Vesna
AU  - Glišić, Dimitrije
AU  - Zurovac Sapundžić, Zorana
AU  - Milovanović, Bojan
AU  - Maletić, Jelena
AU  - Jezdimirović, Nemanja
AU  - Kureljušić, Branislav
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/773
AB  - Contrary to pig farming in developedWestern countries, in a large part of the world, pigs
are still traditionally kept in small backyard farms, usually for family needs. Their main characteristics
are low biosecurity, swill feeding, natural breeding and uncontrolled trade. Given the high number
of backyard farms in Serbia and the risk they are thought to pose to intensive pig farming, the main
aim of this study was to evaluate the prevalence of major viral diseases of swine among traditionally
kept pigs in small holdings with low biosecurity. For this investigation, 222 serum samples from
69 backyard holdings were randomly selected and tested for antibodies to Porcine Reproductive and
Respiratory Syndrome virus (PRRSV), Aujeszky’s disease virus (ADV), Porcine Parvovirus (PPV)
and Swine influenza Virus (SIV) by enzyme-linked immunosorbent assay (ELISA). The herd-level
seroprevalence of PRRS, Aujeszky’s disease and PPV was 2.9%, 27.5% and 37.7%, respectively. Swine
influenza seroconversion was not confirmed in any of the tested holdings. Despite widely distributed
PPV and AD in backyard farms in Serbia, almost 50% of them are still negative for all the tested
diseases. The backyard farms must be monitored, and owners must be educated as their role in
eradication programs and obtaining country-free status may be crucial.
PB  - MDPI
T2  - Animals
T1  - Seroprevalence of viral enzootic diseases in swine backyard farms in Serbia
SP  - 3409
VL  - 13
DO  - 10.3390/ ani13213409
ER  - 

@article{
author = "Milićević, Vesna and Glišić, Dimitrije and Zurovac Sapundžić, Zorana and Milovanović, Bojan and Maletić, Jelena and Jezdimirović, Nemanja and Kureljušić, Branislav",
year = "2023",
abstract = "Contrary to pig farming in developedWestern countries, in a large part of the world, pigs
are still traditionally kept in small backyard farms, usually for family needs. Their main characteristics
are low biosecurity, swill feeding, natural breeding and uncontrolled trade. Given the high number
of backyard farms in Serbia and the risk they are thought to pose to intensive pig farming, the main
aim of this study was to evaluate the prevalence of major viral diseases of swine among traditionally
kept pigs in small holdings with low biosecurity. For this investigation, 222 serum samples from
69 backyard holdings were randomly selected and tested for antibodies to Porcine Reproductive and
Respiratory Syndrome virus (PRRSV), Aujeszky’s disease virus (ADV), Porcine Parvovirus (PPV)
and Swine influenza Virus (SIV) by enzyme-linked immunosorbent assay (ELISA). The herd-level
seroprevalence of PRRS, Aujeszky’s disease and PPV was 2.9%, 27.5% and 37.7%, respectively. Swine
influenza seroconversion was not confirmed in any of the tested holdings. Despite widely distributed
PPV and AD in backyard farms in Serbia, almost 50% of them are still negative for all the tested
diseases. The backyard farms must be monitored, and owners must be educated as their role in
eradication programs and obtaining country-free status may be crucial.",
publisher = "MDPI",
journal = "Animals",
title = "Seroprevalence of viral enzootic diseases in swine backyard farms in Serbia",
pages = "3409",
volume = "13",
doi = "10.3390/ ani13213409"
}

Milićević, V., Glišić, D., Zurovac Sapundžić, Z., Milovanović, B., Maletić, J., Jezdimirović, N.,& Kureljušić, B.. (2023). Seroprevalence of viral enzootic diseases in swine backyard farms in Serbia. in Animals
MDPI., 13, 3409.
https://doi.org/10.3390/ ani13213409

Milićević V, Glišić D, Zurovac Sapundžić Z, Milovanović B, Maletić J, Jezdimirović N, Kureljušić B. Seroprevalence of viral enzootic diseases in swine backyard farms in Serbia. in Animals. 2023;13:3409.
doi:10.3390/ ani13213409 .

Milićević, Vesna, Glišić, Dimitrije, Zurovac Sapundžić, Zorana, Milovanović, Bojan, Maletić, Jelena, Jezdimirović, Nemanja, Kureljušić, Branislav, "Seroprevalence of viral enzootic diseases in swine backyard farms in Serbia" in Animals, 13 (2023):3409,
https://doi.org/10.3390/ ani13213409 . .

TY  - CONF
AU  - Radosavljevic, Vladimir
AU  - Rokvić, Nikola
AU  - Kureljušić, Jasna
AU  - Pavlović, Marija
AU  - Glišić, Dimitrije
AU  - Maletić, Jelena
AU  - Vasić, Ana
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/794
AB  - Poznato je da je anisakijaza jedna od važih zoonoza koje prenose ribe. Ličinke roda Anisakis prema svojim morfološkim značajkama mogu se identificirati kao Anisakis tip I ili tip II. Ličinke Anisakis L3 ranije su prijavljene na srpskim tržištima iz različitih vrsta riba. Uzorak uvezenog duboko smrznutog argentinskog oslića (Merluccius hubbsi, Linnaeus 1758), za kojeg se sumnjalo da je zaražen parazitima, nadležna je sluzba poslala u laboratorij. Parazitoloski pregled uzorka od 10 riba na prisutnost ličinki Anisakis u unutarnjim organima obavljen je vizualno pod stereomikroskopom. Zatim su iznutrice i epaksijalni i hipoksijalni mišići uklonjeni i odvojeno digestirani u otopini pepsin/HCl. Uzorci su potom mikroskopski pregledani u Petrijevim zdjelicama kako bi se otkrile i prebrojale ličinke. U trbušnoj supljini otkriveno je izmedu 10 i 14 ličinki po ribi. Izolirane Anisakis spp. ličinke su isprane u 0,9% otopini NaCl i isprane u 70% alkoholu i ledenoj octenoj kiselini radi mikroskopskog promatranja i morfoloske studije, koja je uključivala bilježenje prisutnosti zuba na prednjem kraju iventrikula jednjaka u prvoj trećini i stražnjem kraju larva, koja varira između morfotipa I i II.Otkrivene ličinke su svjetlosnim mikroskopom identificirane na temelju morfoloskih kriterija kao Anisakis ličinke trećeg stadija (L3) tip I.
AB  - It is known that anisakiasis is one of the important fish-borne zoonotic diseases. Based on their morphological features, larvae of the genus Anisakis can be identified as Anisakis type Ior typeII. The Anisakis L3 larvae had been previously reported in Serbia from different fish species. The sample of imported deep-frozen Argentine hake (Merluccius hubbsi, Linnaeus 1758), which was suspected with parasite infection, was sent to the laboratory by the governing authority. A parasitological examination of sample consisting of 10 fish, for the presence of Anisakis larvae in the viscera was visually performed under a stereomicroscope. Then, the viscera and epaxial and hypaxial muscles were removed and separately digested in pepsin/HCl solution. Samples were then examined microscopically in Petri dishes to detect and count the larvae. Between 10 and 14 larvae per fish were detected in the abdominal cavity. solated Anisakis spp. larvae were washed in 0.9% NaCl solution and rinsed in 70% alcohol and glacial acetic acid for microscopic observation and morphological study, which included notingthe presence of a boringtooth at the anterior end and oesophageal ventricle in the first third and posterior end of the larva, which varies between morphotypes IandII.The detected larvae were identified as based on distinct morphological criteria as Anisakis third-stage larvae (L3) type Ivia light microscopy.
PB  - Zagreb : Hrvatska gospodarska komora
C3  - 14. Međunarodna konferencija o akvakulturi
T1  - Ličinke Anisakis tipa I u argentinskom osliću (Merluccius hubbsi, Linnaeus 1758)
T1  - Anisakis type Ilarvae in Argentine hake (Merluccius hubbsi, Linnaeus 1758)
EP  - 124
SP  - 123
ER  - 

@conference{
author = "Radosavljevic, Vladimir and Rokvić, Nikola and Kureljušić, Jasna and Pavlović, Marija and Glišić, Dimitrije and Maletić, Jelena and Vasić, Ana",
year = "2023",
abstract = "Poznato je da je anisakijaza jedna od važih zoonoza koje prenose ribe. Ličinke roda Anisakis prema svojim morfološkim značajkama mogu se identificirati kao Anisakis tip I ili tip II. Ličinke Anisakis L3 ranije su prijavljene na srpskim tržištima iz različitih vrsta riba. Uzorak uvezenog duboko smrznutog argentinskog oslića (Merluccius hubbsi, Linnaeus 1758), za kojeg se sumnjalo da je zaražen parazitima, nadležna je sluzba poslala u laboratorij. Parazitoloski pregled uzorka od 10 riba na prisutnost ličinki Anisakis u unutarnjim organima obavljen je vizualno pod stereomikroskopom. Zatim su iznutrice i epaksijalni i hipoksijalni mišići uklonjeni i odvojeno digestirani u otopini pepsin/HCl. Uzorci su potom mikroskopski pregledani u Petrijevim zdjelicama kako bi se otkrile i prebrojale ličinke. U trbušnoj supljini otkriveno je izmedu 10 i 14 ličinki po ribi. Izolirane Anisakis spp. ličinke su isprane u 0,9% otopini NaCl i isprane u 70% alkoholu i ledenoj octenoj kiselini radi mikroskopskog promatranja i morfoloske studije, koja je uključivala bilježenje prisutnosti zuba na prednjem kraju iventrikula jednjaka u prvoj trećini i stražnjem kraju larva, koja varira između morfotipa I i II.Otkrivene ličinke su svjetlosnim mikroskopom identificirane na temelju morfoloskih kriterija kao Anisakis ličinke trećeg stadija (L3) tip I., It is known that anisakiasis is one of the important fish-borne zoonotic diseases. Based on their morphological features, larvae of the genus Anisakis can be identified as Anisakis type Ior typeII. The Anisakis L3 larvae had been previously reported in Serbia from different fish species. The sample of imported deep-frozen Argentine hake (Merluccius hubbsi, Linnaeus 1758), which was suspected with parasite infection, was sent to the laboratory by the governing authority. A parasitological examination of sample consisting of 10 fish, for the presence of Anisakis larvae in the viscera was visually performed under a stereomicroscope. Then, the viscera and epaxial and hypaxial muscles were removed and separately digested in pepsin/HCl solution. Samples were then examined microscopically in Petri dishes to detect and count the larvae. Between 10 and 14 larvae per fish were detected in the abdominal cavity. solated Anisakis spp. larvae were washed in 0.9% NaCl solution and rinsed in 70% alcohol and glacial acetic acid for microscopic observation and morphological study, which included notingthe presence of a boringtooth at the anterior end and oesophageal ventricle in the first third and posterior end of the larva, which varies between morphotypes IandII.The detected larvae were identified as based on distinct morphological criteria as Anisakis third-stage larvae (L3) type Ivia light microscopy.",
publisher = "Zagreb : Hrvatska gospodarska komora",
journal = "14. Međunarodna konferencija o akvakulturi",
title = "Ličinke Anisakis tipa I u argentinskom osliću (Merluccius hubbsi, Linnaeus 1758), Anisakis type Ilarvae in Argentine hake (Merluccius hubbsi, Linnaeus 1758)",
pages = "124-123"
}

Radosavljevic, V., Rokvić, N., Kureljušić, J., Pavlović, M., Glišić, D., Maletić, J.,& Vasić, A.. (2023). Ličinke Anisakis tipa I u argentinskom osliću (Merluccius hubbsi, Linnaeus 1758). in 14. Međunarodna konferencija o akvakulturi
Zagreb : Hrvatska gospodarska komora., 123-124.

Radosavljevic V, Rokvić N, Kureljušić J, Pavlović M, Glišić D, Maletić J, Vasić A. Ličinke Anisakis tipa I u argentinskom osliću (Merluccius hubbsi, Linnaeus 1758). in 14. Međunarodna konferencija o akvakulturi. 2023;:123-124..

Radosavljevic, Vladimir, Rokvić, Nikola, Kureljušić, Jasna, Pavlović, Marija, Glišić, Dimitrije, Maletić, Jelena, Vasić, Ana, "Ličinke Anisakis tipa I u argentinskom osliću (Merluccius hubbsi, Linnaeus 1758)" in 14. Međunarodna konferencija o akvakulturi (2023):123-124.

TY  - CONF
AU  - Radosavljevic, Vladimir
AU  - Glišić, Dimitrije
AU  - Radanović, Oliver
AU  - Zdravković, Nemanja
AU  - Maksimović Zorić, Jelena
AU  - Maletić, Jelena
AU  - Veljović, Ljubiša
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/562
AB  - Česta pojava bolesti predstavlja jednu od najznačajnijih prepreka u obezbeđivanju daljeg rasta i održivosti akvakulture, pa su svi učsnici u akvakulturi postali
svesni koliko je proizvodnja ranjiva na namerno ili nenamerno unošenje bolesti i
značaj posledica širenja bolesti na proizvodnju i promet proizvoda akvakulture.
Shodno tome, više zaraznih bolesti je identifikovano kao prioritet za delovanje na
nacionalnom, regionalnom ili međunarodnom nivou. Iz tog razloga, brojne države su razvile i nacionalne biosigurnosne strategije u akvakulturi. Podsticaj za
sprovođenje nacionalnih programa biosigurnosti u akvakulturi je i međunarodna
obaveza države da dokaže da je implementirala biosigurnosne strategije koje su
efikasne u sprečavanju pojave, kontroli i iskorenjivanju bolesti u akvakulturi i da
su ribe i njihovi proizvodi koji potiču iz akvakulture slobodne od određenih bolesti
ili patogena. Kontrola zdravlja riba u akvakulturi Srbije se vrši na osnovu nacionalnog zakonodavstva u koje su implementirani zahtevi Svetske organizacije za
zaštitu zdravlja životinja. U ovom radu je dat pregled implementacije koncepta
biosigurnosti u slatkovodnoj akvakulturi u Republici Srbiji i susednim zemljama.
AB  - Frequent occurrence of diseases represents one of the most signiϔicant obstacles
in ensuring the further growth and sustainability of aquaculture, and all participants in aquaculture production have become aware of production vulnerability
to the intentional or unintentional introduction of diseases and the importance of
the consequences of the spread of diseases on the production and trade of aquaculture products. Consequently, several infectious diseases have been identiϔied as
priorities for action at the national, regional or international level. For this reason,
numerous countries have developed national biosecurity strategies in aquaculture.
The incentive for the implementation of national biosecurity programs in aquaculture is the international obligation of the state to prove that it has implemented  biosecurity strategies that are effective in preventing the occurrence, control and
eradication of diseases in aquaculture and that ϔish and their products originating
from aquaculture are free from certain diseases or pathogens. Fish health control
in Serbian aquaculture is carried out on the basis of national legislation, which has
implemented the requirements of the World Organization for the Protection of Animal Health. This paper provides an overview of the implementation of the concept
of biosecurity in freshwater aquaculture in the Republic of Serbia and neighboring
countries.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za DDD
PB  - Beograd : Fakultet veterinarske medicine, Katedra za zoohigijenu
C3  - 34. Savetovanje Dezinfekcija, dezinsekcija i deratizacija : Jedan svet  - jedno zdravlje
T1  - Biosigurnost u akvakulturi
T1  - Biosecurity in aquaculture
EP  - 123
SP  - 118
ER  - 

@conference{
author = "Radosavljevic, Vladimir and Glišić, Dimitrije and Radanović, Oliver and Zdravković, Nemanja and Maksimović Zorić, Jelena and Maletić, Jelena and Veljović, Ljubiša",
year = "2023",
abstract = "Česta pojava bolesti predstavlja jednu od najznačajnijih prepreka u obezbeđivanju daljeg rasta i održivosti akvakulture, pa su svi učsnici u akvakulturi postali
svesni koliko je proizvodnja ranjiva na namerno ili nenamerno unošenje bolesti i
značaj posledica širenja bolesti na proizvodnju i promet proizvoda akvakulture.
Shodno tome, više zaraznih bolesti je identifikovano kao prioritet za delovanje na
nacionalnom, regionalnom ili međunarodnom nivou. Iz tog razloga, brojne države su razvile i nacionalne biosigurnosne strategije u akvakulturi. Podsticaj za
sprovođenje nacionalnih programa biosigurnosti u akvakulturi je i međunarodna
obaveza države da dokaže da je implementirala biosigurnosne strategije koje su
efikasne u sprečavanju pojave, kontroli i iskorenjivanju bolesti u akvakulturi i da
su ribe i njihovi proizvodi koji potiču iz akvakulture slobodne od određenih bolesti
ili patogena. Kontrola zdravlja riba u akvakulturi Srbije se vrši na osnovu nacionalnog zakonodavstva u koje su implementirani zahtevi Svetske organizacije za
zaštitu zdravlja životinja. U ovom radu je dat pregled implementacije koncepta
biosigurnosti u slatkovodnoj akvakulturi u Republici Srbiji i susednim zemljama., Frequent occurrence of diseases represents one of the most signiϔicant obstacles
in ensuring the further growth and sustainability of aquaculture, and all participants in aquaculture production have become aware of production vulnerability
to the intentional or unintentional introduction of diseases and the importance of
the consequences of the spread of diseases on the production and trade of aquaculture products. Consequently, several infectious diseases have been identiϔied as
priorities for action at the national, regional or international level. For this reason,
numerous countries have developed national biosecurity strategies in aquaculture.
The incentive for the implementation of national biosecurity programs in aquaculture is the international obligation of the state to prove that it has implemented  biosecurity strategies that are effective in preventing the occurrence, control and
eradication of diseases in aquaculture and that ϔish and their products originating
from aquaculture are free from certain diseases or pathogens. Fish health control
in Serbian aquaculture is carried out on the basis of national legislation, which has
implemented the requirements of the World Organization for the Protection of Animal Health. This paper provides an overview of the implementation of the concept
of biosecurity in freshwater aquaculture in the Republic of Serbia and neighboring
countries.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za DDD, Beograd : Fakultet veterinarske medicine, Katedra za zoohigijenu",
journal = "34. Savetovanje Dezinfekcija, dezinsekcija i deratizacija : Jedan svet  - jedno zdravlje",
title = "Biosigurnost u akvakulturi, Biosecurity in aquaculture",
pages = "123-118"
}

Radosavljevic, V., Glišić, D., Radanović, O., Zdravković, N., Maksimović Zorić, J., Maletić, J.,& Veljović, L.. (2023). Biosigurnost u akvakulturi. in 34. Savetovanje Dezinfekcija, dezinsekcija i deratizacija : Jedan svet  - jedno zdravlje
Beograd : Srpsko veterinarsko društvo, Sekcija za DDD., 118-123.

Radosavljevic V, Glišić D, Radanović O, Zdravković N, Maksimović Zorić J, Maletić J, Veljović L. Biosigurnost u akvakulturi. in 34. Savetovanje Dezinfekcija, dezinsekcija i deratizacija : Jedan svet  - jedno zdravlje. 2023;:118-123..

Radosavljevic, Vladimir, Glišić, Dimitrije, Radanović, Oliver, Zdravković, Nemanja, Maksimović Zorić, Jelena, Maletić, Jelena, Veljović, Ljubiša, "Biosigurnost u akvakulturi" in 34. Savetovanje Dezinfekcija, dezinsekcija i deratizacija : Jedan svet  - jedno zdravlje (2023):118-123.

TY  - CONF
AU  - Maletić, Jelena
AU  - Kureljušić, Jasna
AU  - Milovanović, Bojan
AU  - Milićević, Vesna
AU  - Radosavljevic, Vladimir
AU  - Spalević, Ljiljana
AU  - Kureljušić, Branislav
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/551
AB  - Biosigurnost podrazumeva primenu određenih preventivnih mera na farmi u
cilju smanjenja mogućnosti unošenja i širenja patogenih mikroorganizama. Biosigurnost može biti spoljašnja (eksterna) i unutrašnja (interna). Procena biosigurnosti predstavlja kvalitativno ili kvantitativno određivanje nivoa biosigurnosti na
farmama. Istraživanje je sprovedeno na dve brojlerske farme na kojima je procena nivoa biosigurnosti sprovedena upotrebom on-lajn upitnika – Biocheck.Ugent.
Ukupna ocena biosigurnosti na farmi 1 bila je viša za 22% u odnosu na ocenu za
farmu 2. Ocena eksterne biosigurnosti na farmi 1 je za 27% viša u odnosu na ocenu za farmu 2, dok su se ocene za internu biosigurnost razlikovale za 8%. Takođe,
utvrđno je da su ocena eksterne biosigurnosti, kao i ukupna ocena za biosigurnosne mere koje se sprovode na farmi 2, niže u odnosu na svetski prosek dobijen iz
baze podataka Biochek.UGent-a. Dobijene ocene za potkategorije, razlikovale su
se između farmi. Najniže ocene za eksternu biosigurnost dobile su potkategorije
„uklanjanje stajnjaka i leševa” (5/100), „snabdevanje hranom i vodom” (36/100) i
„depopulacija pilića” (51/100), a za internu potkategoriju „čišćenje i dezinfekcija”
(37/100). Sistem za procenu implentiranih biosigurnosnih mera može se posmatrati kao vredan instrument za praćenje nivoa biosigurnosti. Periodična procena
biosigurnosti na brojlerskim farmama na nivou cele zemlje, omogućila bi mapiranje farmi prema nivou biosigurnosti i prema riziku od izbijanja određene bolesti.
AB  - Biosecurity implies the application of certain preventive measures on the farm
in order to reduce the possibility of the introduction and spread of pathogenic
microorganisms. Biosecurity can be external and internal. Biosecurity assessment
is a qualitative or quantitative determination of the level of biosecurity on farms.
The study was conducted on two broiler farms where the biosecurity assessment
was carried out using an online questionnaire – Biocheck.Ugent. The overall biosecurity score on farm 1 was 22% higher than that for farm 2. The external biosecurity score on farm 1 was 27% higher than that for farm 2, while the internal
biosecurity score differed by 8%. The external biosecurity score on farm 2 was
lower than the internal biosecurity score. Also, it was determined that the external
biosecurity, as well as the overall biosecurity implemented on farm 2, had a lower
score compared to the world average score obtained from Biochek.UGent database. Scores obtained for subcategories differed between farms. The lowest scores
for external biosecurity were for the subcategories ”manure and carcass removal” (5/100), ”food and water supply” (36/100), and ”depopulation” (51/100), and
for the internal subcategory ”cleaning and disinfection” (37/100). The system for
evaluating of implemented biosecurity measures can be seen as a valuable instrument for monitoring the level of biosecurity. A periodic assessment of biosecurity
on broiler farms at the country level would enable the mapping of farms according to the level of biosecurity and according to the risk of the outbreak of a certain
disease.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za DDD
PB  - Beograd : Fakultet veterinarske medicine, Katedra za zoohigijenu
C3  - 34. Savetovanje Dezinfekcija, dezinsekcija i deratizacija : Jedan svet  - jedno zdravlje
T1  - Značaj procene nivoa biosigurnosti na brojlerskim farmama
T1  - The importance of biosecurity assessment on the broiler farms
EP  - 109
SP  - 102
ER  - 

@conference{
author = "Maletić, Jelena and Kureljušić, Jasna and Milovanović, Bojan and Milićević, Vesna and Radosavljevic, Vladimir and Spalević, Ljiljana and Kureljušić, Branislav",
year = "2023",
abstract = "Biosigurnost podrazumeva primenu određenih preventivnih mera na farmi u
cilju smanjenja mogućnosti unošenja i širenja patogenih mikroorganizama. Biosigurnost može biti spoljašnja (eksterna) i unutrašnja (interna). Procena biosigurnosti predstavlja kvalitativno ili kvantitativno određivanje nivoa biosigurnosti na
farmama. Istraživanje je sprovedeno na dve brojlerske farme na kojima je procena nivoa biosigurnosti sprovedena upotrebom on-lajn upitnika – Biocheck.Ugent.
Ukupna ocena biosigurnosti na farmi 1 bila je viša za 22% u odnosu na ocenu za
farmu 2. Ocena eksterne biosigurnosti na farmi 1 je za 27% viša u odnosu na ocenu za farmu 2, dok su se ocene za internu biosigurnost razlikovale za 8%. Takođe,
utvrđno je da su ocena eksterne biosigurnosti, kao i ukupna ocena za biosigurnosne mere koje se sprovode na farmi 2, niže u odnosu na svetski prosek dobijen iz
baze podataka Biochek.UGent-a. Dobijene ocene za potkategorije, razlikovale su
se između farmi. Najniže ocene za eksternu biosigurnost dobile su potkategorije
„uklanjanje stajnjaka i leševa” (5/100), „snabdevanje hranom i vodom” (36/100) i
„depopulacija pilića” (51/100), a za internu potkategoriju „čišćenje i dezinfekcija”
(37/100). Sistem za procenu implentiranih biosigurnosnih mera može se posmatrati kao vredan instrument za praćenje nivoa biosigurnosti. Periodična procena
biosigurnosti na brojlerskim farmama na nivou cele zemlje, omogućila bi mapiranje farmi prema nivou biosigurnosti i prema riziku od izbijanja određene bolesti., Biosecurity implies the application of certain preventive measures on the farm
in order to reduce the possibility of the introduction and spread of pathogenic
microorganisms. Biosecurity can be external and internal. Biosecurity assessment
is a qualitative or quantitative determination of the level of biosecurity on farms.
The study was conducted on two broiler farms where the biosecurity assessment
was carried out using an online questionnaire – Biocheck.Ugent. The overall biosecurity score on farm 1 was 22% higher than that for farm 2. The external biosecurity score on farm 1 was 27% higher than that for farm 2, while the internal
biosecurity score differed by 8%. The external biosecurity score on farm 2 was
lower than the internal biosecurity score. Also, it was determined that the external
biosecurity, as well as the overall biosecurity implemented on farm 2, had a lower
score compared to the world average score obtained from Biochek.UGent database. Scores obtained for subcategories differed between farms. The lowest scores
for external biosecurity were for the subcategories ”manure and carcass removal” (5/100), ”food and water supply” (36/100), and ”depopulation” (51/100), and
for the internal subcategory ”cleaning and disinfection” (37/100). The system for
evaluating of implemented biosecurity measures can be seen as a valuable instrument for monitoring the level of biosecurity. A periodic assessment of biosecurity
on broiler farms at the country level would enable the mapping of farms according to the level of biosecurity and according to the risk of the outbreak of a certain
disease.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za DDD, Beograd : Fakultet veterinarske medicine, Katedra za zoohigijenu",
journal = "34. Savetovanje Dezinfekcija, dezinsekcija i deratizacija : Jedan svet  - jedno zdravlje",
title = "Značaj procene nivoa biosigurnosti na brojlerskim farmama, The importance of biosecurity assessment on the broiler farms",
pages = "109-102"
}

Maletić, J., Kureljušić, J., Milovanović, B., Milićević, V., Radosavljevic, V., Spalević, L.,& Kureljušić, B.. (2023). Značaj procene nivoa biosigurnosti na brojlerskim farmama. in 34. Savetovanje Dezinfekcija, dezinsekcija i deratizacija : Jedan svet  - jedno zdravlje
Beograd : Srpsko veterinarsko društvo, Sekcija za DDD., 102-109.

Maletić J, Kureljušić J, Milovanović B, Milićević V, Radosavljevic V, Spalević L, Kureljušić B. Značaj procene nivoa biosigurnosti na brojlerskim farmama. in 34. Savetovanje Dezinfekcija, dezinsekcija i deratizacija : Jedan svet  - jedno zdravlje. 2023;:102-109..

Maletić, Jelena, Kureljušić, Jasna, Milovanović, Bojan, Milićević, Vesna, Radosavljevic, Vladimir, Spalević, Ljiljana, Kureljušić, Branislav, "Značaj procene nivoa biosigurnosti na brojlerskim farmama" in 34. Savetovanje Dezinfekcija, dezinsekcija i deratizacija : Jedan svet  - jedno zdravlje (2023):102-109.

TY  - JOUR
AU  - Kureljušić, Jasna
AU  - Rokvić, Nikola
AU  - Pavlović, Marija
AU  - Tasić, Aleksandra
AU  - Maletić, Jelena
AU  - Ljubojević Pelić, Dragana
AU  - Bijelić, Tanja
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/809
AB  - Food additives are substances of known chemical composition, which are not consumed
as food, nor are they a typical ingredient of food, regardless of nutritional value, but are
added to food with the purpose of improving technological performance and retaining certain
sensory properties. Additives widely used in the food industry include sulfur dioxide
(SO2) and sulfites (E 220 – E 228). Sulfur dioxide and its derivatives are added to food
with the purpose of inhibiting and controlling the growth of microorganisms, preventing
non‑enzymatic browning, inhibiting reactions catalyzed by enzymes, and as antioxidants
and reducing agents. The harmful effects of sulfur dioxide and sulfites are most often associated
with allergic reactions from food, so it is necessary to provide consumers with
information about their presence in food, even when they are found in very small amounts,
because even then the possibility of an allergic reaction is not excluded. This research was
conducted with the aim of determining the amount of sulfites in meat products in the period
from 2019 to 2022. Altogether, 128 meat product samples were analyzed of which 53 were
kebabs and 75 were meat for grilling. After testing, the mean levels of sulfur dioxide and
sulfites in positive samples expressed in mg/kg were 210.0 mg/kg in kebabs and 110.6 mg/
kg in meat for grilling. In conclusion, in most of the tested meat products, the sulfite concentration
was below the established maximum permissible values according to national
and European regulations.
PB  - Belgrade : Institute of Meat Hygiene and Technology
T2  - Meat Technology — Special Issue
T1  - Monitoring of sulfites in kebabs and grilled meat
EP  - 266
IS  - 2
SP  - 263
VL  - 64
DO  - 10.18485/meattech.2023.64.2.48
ER  - 

@article{
author = "Kureljušić, Jasna and Rokvić, Nikola and Pavlović, Marija and Tasić, Aleksandra and Maletić, Jelena and Ljubojević Pelić, Dragana and Bijelić, Tanja",
year = "2023",
abstract = "Food additives are substances of known chemical composition, which are not consumed
as food, nor are they a typical ingredient of food, regardless of nutritional value, but are
added to food with the purpose of improving technological performance and retaining certain
sensory properties. Additives widely used in the food industry include sulfur dioxide
(SO2) and sulfites (E 220 – E 228). Sulfur dioxide and its derivatives are added to food
with the purpose of inhibiting and controlling the growth of microorganisms, preventing
non‑enzymatic browning, inhibiting reactions catalyzed by enzymes, and as antioxidants
and reducing agents. The harmful effects of sulfur dioxide and sulfites are most often associated
with allergic reactions from food, so it is necessary to provide consumers with
information about their presence in food, even when they are found in very small amounts,
because even then the possibility of an allergic reaction is not excluded. This research was
conducted with the aim of determining the amount of sulfites in meat products in the period
from 2019 to 2022. Altogether, 128 meat product samples were analyzed of which 53 were
kebabs and 75 were meat for grilling. After testing, the mean levels of sulfur dioxide and
sulfites in positive samples expressed in mg/kg were 210.0 mg/kg in kebabs and 110.6 mg/
kg in meat for grilling. In conclusion, in most of the tested meat products, the sulfite concentration
was below the established maximum permissible values according to national
and European regulations.",
publisher = "Belgrade : Institute of Meat Hygiene and Technology",
journal = "Meat Technology — Special Issue",
title = "Monitoring of sulfites in kebabs and grilled meat",
pages = "266-263",
number = "2",
volume = "64",
doi = "10.18485/meattech.2023.64.2.48"
}

Kureljušić, J., Rokvić, N., Pavlović, M., Tasić, A., Maletić, J., Ljubojević Pelić, D.,& Bijelić, T.. (2023). Monitoring of sulfites in kebabs and grilled meat. in Meat Technology — Special Issue
Belgrade : Institute of Meat Hygiene and Technology., 64(2), 263-266.
https://doi.org/10.18485/meattech.2023.64.2.48

Kureljušić J, Rokvić N, Pavlović M, Tasić A, Maletić J, Ljubojević Pelić D, Bijelić T. Monitoring of sulfites in kebabs and grilled meat. in Meat Technology — Special Issue. 2023;64(2):263-266.
doi:10.18485/meattech.2023.64.2.48 .

Kureljušić, Jasna, Rokvić, Nikola, Pavlović, Marija, Tasić, Aleksandra, Maletić, Jelena, Ljubojević Pelić, Dragana, Bijelić, Tanja, "Monitoring of sulfites in kebabs and grilled meat" in Meat Technology — Special Issue, 64, no. 2 (2023):263-266,
https://doi.org/10.18485/meattech.2023.64.2.48 . .

TY  - CONF
AU  - Kureljušić, Branislav
AU  - Savić, Božidar
AU  - Milovanović, Bojan
AU  - Maletić, Jelena
AU  - Ninković, Milan
AU  - Jezdimirović, Nemanja
AU  - Prodanov Radulović, Jasna
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/686
AB  - Biosecurity measures together with the efficient on-farm management embraces all aspects of
the prevention of pathogens entering pig farms, regardless of structure and animal number. In
the present study assessment of biosecurity measures in the commercial (industrial) pig farms in
Serbia was carried out through application of the questionnaire to farm veterinarians.
To assess biosecurity in commercial (industrial) farms, a Biocheck online survey
(https://biocheckgent.com) was conducted in the ten commercial pigs farms located in the
Northern part of Serbia where industrial pig production is localized and the highest density of pig
production can be found.
The results of the external biosecurity assessment showed a mean score of 75.55%, compared to
the average in Serbia, which was 68.55%, and the global average of 73%. External biosecurity
assessment showed the lowest scores for feed, water and equipment supply (47%). The
assessment of internal biosecurity revealed a score of 54.89% compared to the national average
of 44.22% and the world average of 64.11%. Regarding internal biosecurity, the lowest
percentage score (37.22%) was confirmed for farrowing and suckling period and for finishing unit
(37.67%). The overall biosecurity score for commercial pig farms included in this study was
65.55%. The national average was 56.33%, while the global average was 68.67%.
This study showed a bit less lower level of biosecurity measures in commercial pig farms in Serbia
comparing with global average. Certainly, having in mind the different pig production systems in
Serbia, there is urgent need to improve biosecurity in industrial farms. Serbia as a Western Balkan
country, has variable pig farming system characterized by a domination of traditional family
farms, smallholdings, backyard production and semi-free and free-range. Currently, traditional
farming methods in Serbia represent a cultural identity. The biosecurity measures that could be
implemented in extisted animal production systems need to be urgently identified.
PB  - Ghent : Faculty of veterinary medicine
C3  - COST action CA20103 "Biosecurity Enhanced Through Training Evaluation and Raising Awareness"
T1  - Assessment of biosecurity measures in the commercial (industrial) pig farms in Serbia
EP  - 39
SP  - 39
ER  - 

@conference{
author = "Kureljušić, Branislav and Savić, Božidar and Milovanović, Bojan and Maletić, Jelena and Ninković, Milan and Jezdimirović, Nemanja and Prodanov Radulović, Jasna",
year = "2023",
abstract = "Biosecurity measures together with the efficient on-farm management embraces all aspects of
the prevention of pathogens entering pig farms, regardless of structure and animal number. In
the present study assessment of biosecurity measures in the commercial (industrial) pig farms in
Serbia was carried out through application of the questionnaire to farm veterinarians.
To assess biosecurity in commercial (industrial) farms, a Biocheck online survey
(https://biocheckgent.com) was conducted in the ten commercial pigs farms located in the
Northern part of Serbia where industrial pig production is localized and the highest density of pig
production can be found.
The results of the external biosecurity assessment showed a mean score of 75.55%, compared to
the average in Serbia, which was 68.55%, and the global average of 73%. External biosecurity
assessment showed the lowest scores for feed, water and equipment supply (47%). The
assessment of internal biosecurity revealed a score of 54.89% compared to the national average
of 44.22% and the world average of 64.11%. Regarding internal biosecurity, the lowest
percentage score (37.22%) was confirmed for farrowing and suckling period and for finishing unit
(37.67%). The overall biosecurity score for commercial pig farms included in this study was
65.55%. The national average was 56.33%, while the global average was 68.67%.
This study showed a bit less lower level of biosecurity measures in commercial pig farms in Serbia
comparing with global average. Certainly, having in mind the different pig production systems in
Serbia, there is urgent need to improve biosecurity in industrial farms. Serbia as a Western Balkan
country, has variable pig farming system characterized by a domination of traditional family
farms, smallholdings, backyard production and semi-free and free-range. Currently, traditional
farming methods in Serbia represent a cultural identity. The biosecurity measures that could be
implemented in extisted animal production systems need to be urgently identified.",
publisher = "Ghent : Faculty of veterinary medicine",
journal = "COST action CA20103 "Biosecurity Enhanced Through Training Evaluation and Raising Awareness"",
title = "Assessment of biosecurity measures in the commercial (industrial) pig farms in Serbia",
pages = "39-39"
}

Kureljušić, B., Savić, B., Milovanović, B., Maletić, J., Ninković, M., Jezdimirović, N.,& Prodanov Radulović, J.. (2023). Assessment of biosecurity measures in the commercial (industrial) pig farms in Serbia. in COST action CA20103 "Biosecurity Enhanced Through Training Evaluation and Raising Awareness"
Ghent : Faculty of veterinary medicine., 39-39.

Kureljušić B, Savić B, Milovanović B, Maletić J, Ninković M, Jezdimirović N, Prodanov Radulović J. Assessment of biosecurity measures in the commercial (industrial) pig farms in Serbia. in COST action CA20103 "Biosecurity Enhanced Through Training Evaluation and Raising Awareness". 2023;:39-39..

Kureljušić, Branislav, Savić, Božidar, Milovanović, Bojan, Maletić, Jelena, Ninković, Milan, Jezdimirović, Nemanja, Prodanov Radulović, Jasna, "Assessment of biosecurity measures in the commercial (industrial) pig farms in Serbia" in COST action CA20103 "Biosecurity Enhanced Through Training Evaluation and Raising Awareness" (2023):39-39.

TY  - CONF
AU  - Maksimović Zorić, Jelena
AU  - Veljović, Ljubiša
AU  - Glišić, Dimitrije
AU  - Jezdimirović, Nemanja
AU  - Savić, Božidar
AU  - Milovanović, Bojan
AU  - Maletić, Jelena
AU  - Radosavljević, Vladimir
AU  - Kureljušić, Branislav
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/652
AB  - Swine influenza is a worldwide present respiratory disease of swine, in majority of cases caused by H1N1,
H1N2, and H3N2 subtypes of swine influenza A virus (swIAV). Exploration of the swIAV in Serbia in recent
ten years mainly was oriented to the passive surveillance and examination within PRDC on commercial pig
farms. The latest results suggest circulation of the viruses in the majority of the analysed commercial farms
and the presence of two subtypes - H1N1 and H3N2. Even though the problem exists none of the farms
apply vaccination as a measure of disease control. The aim of this research is to analyse the results of
passive surveillance on the two commercial farms conducted from 2020th to 2022nd.
A total of 94 samples for molecular analyses and 528 samples for serological analyses were submitted to
the Institute of Veterinary Medicine of Serbia during this period. These samples were obtained from swine
raised in two commercial farms (farm A and farm B) that practice farrow-to-finish production and have
implemented satisfactory biosecurity measures. The samples for molecular examination were mummified
fetuses, nasal swabs, oral fluids, parenchymatous organs, lungs, and sera. All samples were tested by M
gene real-time RT-PCR, according to the accredited WHO protocol (2009) for detection of influenza A
viruses. One virus was typed using multiplex real-time RT-PCR protocols described by Henritzi et al. (2016).
Serological examination was conducted using ELISA test (Ingezim Influenza A Kit, Ingenasa), and tested sera
originated from sows, suckling piglets, and nursing pigs.
During three year period influenza A viruses were detected on both farms, having on average 55,3% of the
analyzed samples positive. The highest detection rate was amongst the nasal swab (78.7%) and oral fluid
samples (83.3%), while virus was not confirmed in mummified fetuses and sera. Most of the samples
analyzed by PCR originated from farm A (80 samples), and the majority of tested were positive (60%).
Molecular typing revealed presence of H1N1 subtype in farm A that is in accordance with previous results
that confirmed H1N1 as dominating subtype that circulates in commercial farms in Serbia. Serology
examination confirmed a high overall seroprevalence of 64,6%. The serum samples from nursing pigs
accounted for the highest proportion (95.4%) among those examined, as respiratory issues linked to PRDC
typically manifest in this group. Given the endemic presence of swIAVs in the farms under analysis, there is
a pressing need to conduct further research on the circulating viruses and enhance the control measures
employed on these farms.
PB  - ESFLU
C3  - ESFLU Scientific meeting, Barcelona, 25-27th April 2023
T1  - Serological and molecular investigation of influenza in commercial pig farms in Serbia
ER  - 

@conference{
author = "Maksimović Zorić, Jelena and Veljović, Ljubiša and Glišić, Dimitrije and Jezdimirović, Nemanja and Savić, Božidar and Milovanović, Bojan and Maletić, Jelena and Radosavljević, Vladimir and Kureljušić, Branislav",
year = "2023",
abstract = "Swine influenza is a worldwide present respiratory disease of swine, in majority of cases caused by H1N1,
H1N2, and H3N2 subtypes of swine influenza A virus (swIAV). Exploration of the swIAV in Serbia in recent
ten years mainly was oriented to the passive surveillance and examination within PRDC on commercial pig
farms. The latest results suggest circulation of the viruses in the majority of the analysed commercial farms
and the presence of two subtypes - H1N1 and H3N2. Even though the problem exists none of the farms
apply vaccination as a measure of disease control. The aim of this research is to analyse the results of
passive surveillance on the two commercial farms conducted from 2020th to 2022nd.
A total of 94 samples for molecular analyses and 528 samples for serological analyses were submitted to
the Institute of Veterinary Medicine of Serbia during this period. These samples were obtained from swine
raised in two commercial farms (farm A and farm B) that practice farrow-to-finish production and have
implemented satisfactory biosecurity measures. The samples for molecular examination were mummified
fetuses, nasal swabs, oral fluids, parenchymatous organs, lungs, and sera. All samples were tested by M
gene real-time RT-PCR, according to the accredited WHO protocol (2009) for detection of influenza A
viruses. One virus was typed using multiplex real-time RT-PCR protocols described by Henritzi et al. (2016).
Serological examination was conducted using ELISA test (Ingezim Influenza A Kit, Ingenasa), and tested sera
originated from sows, suckling piglets, and nursing pigs.
During three year period influenza A viruses were detected on both farms, having on average 55,3% of the
analyzed samples positive. The highest detection rate was amongst the nasal swab (78.7%) and oral fluid
samples (83.3%), while virus was not confirmed in mummified fetuses and sera. Most of the samples
analyzed by PCR originated from farm A (80 samples), and the majority of tested were positive (60%).
Molecular typing revealed presence of H1N1 subtype in farm A that is in accordance with previous results
that confirmed H1N1 as dominating subtype that circulates in commercial farms in Serbia. Serology
examination confirmed a high overall seroprevalence of 64,6%. The serum samples from nursing pigs
accounted for the highest proportion (95.4%) among those examined, as respiratory issues linked to PRDC
typically manifest in this group. Given the endemic presence of swIAVs in the farms under analysis, there is
a pressing need to conduct further research on the circulating viruses and enhance the control measures
employed on these farms.",
publisher = "ESFLU",
journal = "ESFLU Scientific meeting, Barcelona, 25-27th April 2023",
title = "Serological and molecular investigation of influenza in commercial pig farms in Serbia"
}

Maksimović Zorić, J., Veljović, L., Glišić, D., Jezdimirović, N., Savić, B., Milovanović, B., Maletić, J., Radosavljević, V.,& Kureljušić, B.. (2023). Serological and molecular investigation of influenza in commercial pig farms in Serbia. in ESFLU Scientific meeting, Barcelona, 25-27th April 2023
ESFLU..

Maksimović Zorić J, Veljović L, Glišić D, Jezdimirović N, Savić B, Milovanović B, Maletić J, Radosavljević V, Kureljušić B. Serological and molecular investigation of influenza in commercial pig farms in Serbia. in ESFLU Scientific meeting, Barcelona, 25-27th April 2023. 2023;..

Maksimović Zorić, Jelena, Veljović, Ljubiša, Glišić, Dimitrije, Jezdimirović, Nemanja, Savić, Božidar, Milovanović, Bojan, Maletić, Jelena, Radosavljević, Vladimir, Kureljušić, Branislav, "Serological and molecular investigation of influenza in commercial pig farms in Serbia" in ESFLU Scientific meeting, Barcelona, 25-27th April 2023 (2023).

TY  - CONF
AU  - Glišić, Dimitrije
AU  - Veljović, Ljubiša
AU  - Maletić, Jelena
AU  - Kureljušić, Branislav
AU  - Ninković, Milan
AU  - Milovanović, Bojan
AU  - Milićević, Vesna
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/540
AB  - Mnoge zarazne bolesti se prenose vazduhom. Jedinke koje pokazuju kliničke
simptome respiratornih bolesti izlučuju uzročnika u sekretu, zatim
iskašljavanjem u vidu kapljica uzročnici dospevaju u spoljašnju sredinu u
kojoj mogu opstati zaštićeni unutar kapljica. Crevni patogeni se takođe mogu
širiti putem vazduha uz pomoć čestica prašine koje se podižu aktivnim
kretanjem životinja i osoblja na farmi. Neinvazivne metode uzorkovanja su
vrlo značajne za brz i jednostavan uvid u zdravstveno stanje zapata,
isključujući individualne varijacije između jedinki, i ujedno neometajući
redovne aktivnosti na farmi. Ranije je pokazano da je oralna tečnost kao uzorak
dobijen neinvazivnim putem veoma pouzdan za utvrđivanje statusa zapata.
Međutim, u našoj studiji, ispitali smo prisustvo virusnih patogena svinja u
vazduhu, uključujući virus influence svinja, cirkovirus tip dva svinja kao i
virus respiratornog i reproduktivnog sindroma svinja. Vazduh je uzorkovan
upotrebom „Aerokolekt“ uređaja. Princip rada „Aerokolekt“ uređaja je
zasnovan na strujanju vazduha kroz jednokratan čip iz kog se u laboratoriji
direktnom elucijom dobija uzorak nukleinske kiseline pogodan za PCR
analize. Uzorci su sakupljeni sa 5 farmi svinja koje imaju potvrđeno prisustvo
navedenih virusnih patogena. Rezultati ispitivanja vazduha su poređeni sa
rezultatima dobijenim iz uobičajeno sakupljenih uzoraka. Preliminarnim
rezultatima je utvrđeno da je ispitivanje vazduha pogodno za utvrđivanje
prisustva navedenih uzročnika na farmi. Primenom metodologije uzorkovanja
vazduha, može se vršiti aktivan nadzor na prisustvo patogena, održavanje
slobodnog statusa od bolesti, kao i rana detekcija patogena. Značajne
prednosti ispitivanja vazduha su i očuvanje dobrobiti, usled izostanka
direktnog kontakta sa životinjama.
AB  - Many infectious diseases are transmitted by air. Individuals which show clinical signs
of respiratory diseases excrete the culprit, and through expectoration in the form of
droplets, the causative agents end up in the external environment where they can
remain be protected within the droplets. Enteral pathogens can also be transmitted by
air, through dust particles lifted by active animal and farm staff movement. Noninvasive sampling methods are significant for swift and simple insight into the health
status of a herd, excluding individual variations, and withal not disrupting regular
farm activities. Earlier it was shown that oral fluid samples, acquired by non-invasive
methods, are a reliable way of determining the status of a herd. Meanwhile, our study
tested the presence of viral swine pathogens in the air, including the swine influenza
virus, circovirus type two, and the respiratory and reproductive syndrome virus. The
air was sampled using the “Aerocollect” device. The working principle of the
“Aerocollect” apparatus is based on the flow of air through a single-use chip from
which nucleic acid can be eluted and used for PCR analysis. The samples were
collected from five pig farms, which had a confirmed presence of the beforementioned
viral swine pathogens. The results of the analysis were compared with results from
traditionally collected samples. The preliminary results confirm that air sampling can
be used for determining the presence of the mentioned pathogens on a farm. By
applying air sampling methodology, active surveillance for the presence of pathogens
can be conducted, as well as the maintenance of free disease status, and early pathogen
detection. Considerable advantages of air sampling are the preservation of animal
welfare and the absence of direct animal contact.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani)
T1  - Virusni patogeni svinja u vazduhu
T1  - Viral swine pathogens in the air
EP  - 85
SP  - 84
ER  - 

@conference{
author = "Glišić, Dimitrije and Veljović, Ljubiša and Maletić, Jelena and Kureljušić, Branislav and Ninković, Milan and Milovanović, Bojan and Milićević, Vesna",
year = "2023",
abstract = "Mnoge zarazne bolesti se prenose vazduhom. Jedinke koje pokazuju kliničke
simptome respiratornih bolesti izlučuju uzročnika u sekretu, zatim
iskašljavanjem u vidu kapljica uzročnici dospevaju u spoljašnju sredinu u
kojoj mogu opstati zaštićeni unutar kapljica. Crevni patogeni se takođe mogu
širiti putem vazduha uz pomoć čestica prašine koje se podižu aktivnim
kretanjem životinja i osoblja na farmi. Neinvazivne metode uzorkovanja su
vrlo značajne za brz i jednostavan uvid u zdravstveno stanje zapata,
isključujući individualne varijacije između jedinki, i ujedno neometajući
redovne aktivnosti na farmi. Ranije je pokazano da je oralna tečnost kao uzorak
dobijen neinvazivnim putem veoma pouzdan za utvrđivanje statusa zapata.
Međutim, u našoj studiji, ispitali smo prisustvo virusnih patogena svinja u
vazduhu, uključujući virus influence svinja, cirkovirus tip dva svinja kao i
virus respiratornog i reproduktivnog sindroma svinja. Vazduh je uzorkovan
upotrebom „Aerokolekt“ uređaja. Princip rada „Aerokolekt“ uređaja je
zasnovan na strujanju vazduha kroz jednokratan čip iz kog se u laboratoriji
direktnom elucijom dobija uzorak nukleinske kiseline pogodan za PCR
analize. Uzorci su sakupljeni sa 5 farmi svinja koje imaju potvrđeno prisustvo
navedenih virusnih patogena. Rezultati ispitivanja vazduha su poređeni sa
rezultatima dobijenim iz uobičajeno sakupljenih uzoraka. Preliminarnim
rezultatima je utvrđeno da je ispitivanje vazduha pogodno za utvrđivanje
prisustva navedenih uzročnika na farmi. Primenom metodologije uzorkovanja
vazduha, može se vršiti aktivan nadzor na prisustvo patogena, održavanje
slobodnog statusa od bolesti, kao i rana detekcija patogena. Značajne
prednosti ispitivanja vazduha su i očuvanje dobrobiti, usled izostanka
direktnog kontakta sa životinjama., Many infectious diseases are transmitted by air. Individuals which show clinical signs
of respiratory diseases excrete the culprit, and through expectoration in the form of
droplets, the causative agents end up in the external environment where they can
remain be protected within the droplets. Enteral pathogens can also be transmitted by
air, through dust particles lifted by active animal and farm staff movement. Noninvasive sampling methods are significant for swift and simple insight into the health
status of a herd, excluding individual variations, and withal not disrupting regular
farm activities. Earlier it was shown that oral fluid samples, acquired by non-invasive
methods, are a reliable way of determining the status of a herd. Meanwhile, our study
tested the presence of viral swine pathogens in the air, including the swine influenza
virus, circovirus type two, and the respiratory and reproductive syndrome virus. The
air was sampled using the “Aerocollect” device. The working principle of the
“Aerocollect” apparatus is based on the flow of air through a single-use chip from
which nucleic acid can be eluted and used for PCR analysis. The samples were
collected from five pig farms, which had a confirmed presence of the beforementioned
viral swine pathogens. The results of the analysis were compared with results from
traditionally collected samples. The preliminary results confirm that air sampling can
be used for determining the presence of the mentioned pathogens on a farm. By
applying air sampling methodology, active surveillance for the presence of pathogens
can be conducted, as well as the maintenance of free disease status, and early pathogen
detection. Considerable advantages of air sampling are the preservation of animal
welfare and the absence of direct animal contact.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani)",
title = "Virusni patogeni svinja u vazduhu, Viral swine pathogens in the air",
pages = "85-84"
}

Glišić, D., Veljović, L., Maletić, J., Kureljušić, B., Ninković, M., Milovanović, B.,& Milićević, V.. (2023). Virusni patogeni svinja u vazduhu. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani)
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 84-85.

Glišić D, Veljović L, Maletić J, Kureljušić B, Ninković M, Milovanović B, Milićević V. Virusni patogeni svinja u vazduhu. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani). 2023;:84-85..

Glišić, Dimitrije, Veljović, Ljubiša, Maletić, Jelena, Kureljušić, Branislav, Ninković, Milan, Milovanović, Bojan, Milićević, Vesna, "Virusni patogeni svinja u vazduhu" in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani) (2023):84-85.

TY  - CONF
AU  - Maksimović Zorić, Jelena
AU  - Veljović, Ljubiša
AU  - Glišić, Dimitrije
AU  - Savić, Božidar
AU  - Jezdimirović, Nemanja
AU  - Milovanović, Bojan
AU  - Maletić, Jelena
AU  - Spalević, Ljiljana
AU  - Kureljušić, Branislav
PY  - 2023
UR  - https://reponivs.nivs.rs/handle/123456789/505
AB  - Influenca svinja predstavlja veoma važan zdravstvni i ekonomski problem u
komercijalnom uzgoju svinja. U etiologiji respiratornih oboljenja do kojih dovode dominiraju tri podtipa virusa influence tipa A - H1N1, H1N2 i H3N2, različitih genetskih i antigenskih karakteristika (Detmer, 2017). Iako u neimunom zapatu influenca može ostvariti visok morbiditet (i do 100%), mortalitet je uglavnom nizak (manji od 1%) i zavisi od soja virusa i prisustva drugih infekcija u zapatu (Van Reeth i sar., 2012, Zell i sar., 2013). Kao posledica cirkulacije virusa na
farmi dolazi do direktnih, i mnogo značajnijih, indirektnih gubitaka koji se ogledaju u smanjenju dnevnog prirasta, povećanju utroška hrane za kilogram prirasta, produžetku tova i dr. (Došen i sar., 2008). Na ekonomičnost proizvodnje najznačajnije utiče smanjenje dnevnog prirasta (Calderón Díaz i sar., 2020), a Haden i sar., 2012, su ustanovili da gubici po grlu u slučaju monoinfekcije virusom influence iznose 3,23$. Pored direktnih i indirektnih gubitaka u
proizvodnji, cirkulacija virusa influence u populacijama svinja predstavlja i problem od javnog značaja zbog zoonotskog potencijala koji ovi virusi poseduju i mogućih uzrokovanja oboljenja ljudi. Posle pandemije 2009. godine za koju je utvrđeno da je nastala prilagođavanjem i širenjem reasortiranog virusa svinja u humanoj populaciji (Smith i sar., 2009) opisani su mnogobrojni slučajevi oboljenja ljudi nastali prenošenjem virusa sa svinja na ljude (Hennig i sar., 2022). Iako
su to najčešće incidentni slučajevi oboljenja, a tek u nekoliko navrata oboljenje manje grupe ljudi (Hennig i sar., 2022), potencijal za prenos svinjskih virusa u humanu populaciju je evidentan, što upućuje na neophodnost praćenja raširenosti ove infekcije u zapatima svinja primenom kliničke opservacije i laboratorijskih ispitivanja obolelih.
Cirkulaciju virusa i stepen raširenosti infekcije u jednom zapatu svinja u kome se ne primenjuje vakcinacija kao mera kontrole, najlakše i najekonomičnije je utvrditi primenom laboratorijskog ispitivanja prisustva specifičnih antitela protiv virusa influence tipa A. Imunološki odgovor u vidu sinteze antitela je detektabilan već trećeg dana od nastanka infekcije (Lee i sar., 1995), pri čemu pik dostiže obično između 14. i 21. dana (Radojičić i sar., 2011). Nakon primarne infekcije nastali imunitet je dugotrajan i pruža zaštitu od infekcije istim ili drugim antigeno
sličnim sojem (Van Reeth i sar., 2012). Humoralni imunološki odgovor se ogleda u sintezi specifičnih antitela protiv hemaglutinina, neuraminidaze, nukleoproteina i matriks proteina virusa (Wright i sar., 2007), a laboratorijske metode koje se koriste za njihovu detekciju su imunoenzimski testovi (ELISA), virus neutralizacioni test (VNT) i inhibibicija hemaglutinacije (IHA) (Detmer i sar., 2013). Za testiranje velikog broja uzoraka seruma koje za cilj ima utvrđivanje seroprevalencije u jednom zapatu najpogodnije je koristiti ELISA test, dok se titar
antitela kod pojedinačnih životinja kao i podtip cirkulišućih virusa utvrđuju primenom VNT ili IHA. S' obzirom na činjenicu da je cirkulacija virusa influence tipa A potvrđena na većini velikih komercijalnih farmi u Srbiji (Maksimović Zorić i sar., 2020), izvršeno je ispitivanje seroprevalencije u različitim uzrasnim kategorijama i serotipizacija cirkulišućih virusa.
PB  - Požarevac : Sitograf RM
C3  - 20. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja
T1  - Seroloska ispitivanja influence kod razlicitih uzrasnih kategorija svinja uzgajanih na komercijalnim farmama
EP  - 55
SP  - 48
ER  - 

@conference{
author = "Maksimović Zorić, Jelena and Veljović, Ljubiša and Glišić, Dimitrije and Savić, Božidar and Jezdimirović, Nemanja and Milovanović, Bojan and Maletić, Jelena and Spalević, Ljiljana and Kureljušić, Branislav",
year = "2023",
abstract = "Influenca svinja predstavlja veoma važan zdravstvni i ekonomski problem u
komercijalnom uzgoju svinja. U etiologiji respiratornih oboljenja do kojih dovode dominiraju tri podtipa virusa influence tipa A - H1N1, H1N2 i H3N2, različitih genetskih i antigenskih karakteristika (Detmer, 2017). Iako u neimunom zapatu influenca može ostvariti visok morbiditet (i do 100%), mortalitet je uglavnom nizak (manji od 1%) i zavisi od soja virusa i prisustva drugih infekcija u zapatu (Van Reeth i sar., 2012, Zell i sar., 2013). Kao posledica cirkulacije virusa na
farmi dolazi do direktnih, i mnogo značajnijih, indirektnih gubitaka koji se ogledaju u smanjenju dnevnog prirasta, povećanju utroška hrane za kilogram prirasta, produžetku tova i dr. (Došen i sar., 2008). Na ekonomičnost proizvodnje najznačajnije utiče smanjenje dnevnog prirasta (Calderón Díaz i sar., 2020), a Haden i sar., 2012, su ustanovili da gubici po grlu u slučaju monoinfekcije virusom influence iznose 3,23$. Pored direktnih i indirektnih gubitaka u
proizvodnji, cirkulacija virusa influence u populacijama svinja predstavlja i problem od javnog značaja zbog zoonotskog potencijala koji ovi virusi poseduju i mogućih uzrokovanja oboljenja ljudi. Posle pandemije 2009. godine za koju je utvrđeno da je nastala prilagođavanjem i širenjem reasortiranog virusa svinja u humanoj populaciji (Smith i sar., 2009) opisani su mnogobrojni slučajevi oboljenja ljudi nastali prenošenjem virusa sa svinja na ljude (Hennig i sar., 2022). Iako
su to najčešće incidentni slučajevi oboljenja, a tek u nekoliko navrata oboljenje manje grupe ljudi (Hennig i sar., 2022), potencijal za prenos svinjskih virusa u humanu populaciju je evidentan, što upućuje na neophodnost praćenja raširenosti ove infekcije u zapatima svinja primenom kliničke opservacije i laboratorijskih ispitivanja obolelih.
Cirkulaciju virusa i stepen raširenosti infekcije u jednom zapatu svinja u kome se ne primenjuje vakcinacija kao mera kontrole, najlakše i najekonomičnije je utvrditi primenom laboratorijskog ispitivanja prisustva specifičnih antitela protiv virusa influence tipa A. Imunološki odgovor u vidu sinteze antitela je detektabilan već trećeg dana od nastanka infekcije (Lee i sar., 1995), pri čemu pik dostiže obično između 14. i 21. dana (Radojičić i sar., 2011). Nakon primarne infekcije nastali imunitet je dugotrajan i pruža zaštitu od infekcije istim ili drugim antigeno
sličnim sojem (Van Reeth i sar., 2012). Humoralni imunološki odgovor se ogleda u sintezi specifičnih antitela protiv hemaglutinina, neuraminidaze, nukleoproteina i matriks proteina virusa (Wright i sar., 2007), a laboratorijske metode koje se koriste za njihovu detekciju su imunoenzimski testovi (ELISA), virus neutralizacioni test (VNT) i inhibibicija hemaglutinacije (IHA) (Detmer i sar., 2013). Za testiranje velikog broja uzoraka seruma koje za cilj ima utvrđivanje seroprevalencije u jednom zapatu najpogodnije je koristiti ELISA test, dok se titar
antitela kod pojedinačnih životinja kao i podtip cirkulišućih virusa utvrđuju primenom VNT ili IHA. S' obzirom na činjenicu da je cirkulacija virusa influence tipa A potvrđena na većini velikih komercijalnih farmi u Srbiji (Maksimović Zorić i sar., 2020), izvršeno je ispitivanje seroprevalencije u različitim uzrasnim kategorijama i serotipizacija cirkulišućih virusa.",
publisher = "Požarevac : Sitograf RM",
journal = "20. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja",
title = "Seroloska ispitivanja influence kod razlicitih uzrasnih kategorija svinja uzgajanih na komercijalnim farmama",
pages = "55-48"
}

Maksimović Zorić, J., Veljović, L., Glišić, D., Savić, B., Jezdimirović, N., Milovanović, B., Maletić, J., Spalević, L.,& Kureljušić, B.. (2023). Seroloska ispitivanja influence kod razlicitih uzrasnih kategorija svinja uzgajanih na komercijalnim farmama. in 20. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja
Požarevac : Sitograf RM., 48-55.

Maksimović Zorić J, Veljović L, Glišić D, Savić B, Jezdimirović N, Milovanović B, Maletić J, Spalević L, Kureljušić B. Seroloska ispitivanja influence kod razlicitih uzrasnih kategorija svinja uzgajanih na komercijalnim farmama. in 20. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja. 2023;:48-55..

Maksimović Zorić, Jelena, Veljović, Ljubiša, Glišić, Dimitrije, Savić, Božidar, Jezdimirović, Nemanja, Milovanović, Bojan, Maletić, Jelena, Spalević, Ljiljana, Kureljušić, Branislav, "Seroloska ispitivanja influence kod razlicitih uzrasnih kategorija svinja uzgajanih na komercijalnim farmama" in 20. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja (2023):48-55.