Ivetić, Vojin

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Comparative pathomorphological, mycological and molecular examination of turkey poults with different immunological status experimentally infected with aspergillus fumigatus

Jezdimirović, Nemanja; Kureljušić, Branislav; Ivetić, Vojin; Krnjaić, Dejan; Radanović, Oliver; Žutić, Jadranka; Spalević, Ljiljana; Jovanović, Milijan

(Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd, 2019)

TY  - JOUR
AU  - Jezdimirović, Nemanja
AU  - Kureljušić, Branislav
AU  - Ivetić, Vojin
AU  - Krnjaić, Dejan
AU  - Radanović, Oliver
AU  - Žutić, Jadranka
AU  - Spalević, Ljiljana
AU  - Jovanović, Milijan
PY  - 2019
UR  - https://reponivs.nivs.rs/handle/123456789/396
AB  - The aim of this study was to determine the pathological, mycological and molecular findings in turkey poults with different immunological status experimentally infected with Aspergillus fumigatus. The investigation was carried out 1, 3, 7, 14 and 21 days after intratracheal inoculation of 5.056x10(7) spores of A. fumigatus to 14-day-old turkey poults in group G-1, as well as to turkey poults in group G-2 which were treated prior to infection with dexamethasone. A. fumigatus was isolated on day 1 p.i. in both groups, but the number of positive samples was bigger in group G-1. A. fumigatus was isolated from the respiratory organs of group G-1as early as on day 1 and 3 p.i. in 4 out of 12 examined specimens (33%). On day 7 p.i. A. fumigatus was possible to isolate from the respiratory organs of 50% of infected birds, on day 14 in 83.33% and on day 21 p.i. A. fumigatus was isolated in 6 out of 6 sacrificed turkey poults (100%). In dexamethasone-treated group A. fumigatus isolates from the respiratory organs on day 1 and 3 p.i. were same as in group G-1, whereas on days 7 and 14 p.i. the number of turkey poults positive to A. fumigatus increased in comparison with the untreated G-1 group. The histopathological lesions in turkey poults treated with dexamethasone developed earlier, were more intensive and extensive. The mycological and nested PCR results revealed a higher number of samples positive for the presence of A. fumigatus DNA in the group G-2, pretreated with dexamethasone.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Comparative pathomorphological, mycological and molecular examination of turkey poults with different immunological status experimentally infected with aspergillus fumigatus
EP  - 217
IS  - 2
SP  - 201
VL  - 69
DO  - 10.2478/acve-2019-0016
UR  - conv_524
ER  - 
@article{
author = "Jezdimirović, Nemanja and Kureljušić, Branislav and Ivetić, Vojin and Krnjaić, Dejan and Radanović, Oliver and Žutić, Jadranka and Spalević, Ljiljana and Jovanović, Milijan",
year = "2019",
abstract = "The aim of this study was to determine the pathological, mycological and molecular findings in turkey poults with different immunological status experimentally infected with Aspergillus fumigatus. The investigation was carried out 1, 3, 7, 14 and 21 days after intratracheal inoculation of 5.056x10(7) spores of A. fumigatus to 14-day-old turkey poults in group G-1, as well as to turkey poults in group G-2 which were treated prior to infection with dexamethasone. A. fumigatus was isolated on day 1 p.i. in both groups, but the number of positive samples was bigger in group G-1. A. fumigatus was isolated from the respiratory organs of group G-1as early as on day 1 and 3 p.i. in 4 out of 12 examined specimens (33%). On day 7 p.i. A. fumigatus was possible to isolate from the respiratory organs of 50% of infected birds, on day 14 in 83.33% and on day 21 p.i. A. fumigatus was isolated in 6 out of 6 sacrificed turkey poults (100%). In dexamethasone-treated group A. fumigatus isolates from the respiratory organs on day 1 and 3 p.i. were same as in group G-1, whereas on days 7 and 14 p.i. the number of turkey poults positive to A. fumigatus increased in comparison with the untreated G-1 group. The histopathological lesions in turkey poults treated with dexamethasone developed earlier, were more intensive and extensive. The mycological and nested PCR results revealed a higher number of samples positive for the presence of A. fumigatus DNA in the group G-2, pretreated with dexamethasone.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Comparative pathomorphological, mycological and molecular examination of turkey poults with different immunological status experimentally infected with aspergillus fumigatus",
pages = "217-201",
number = "2",
volume = "69",
doi = "10.2478/acve-2019-0016",
url = "conv_524"
}
Jezdimirović, N., Kureljušić, B., Ivetić, V., Krnjaić, D., Radanović, O., Žutić, J., Spalević, L.,& Jovanović, M.. (2019). Comparative pathomorphological, mycological and molecular examination of turkey poults with different immunological status experimentally infected with aspergillus fumigatus. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 69(2), 201-217.
https://doi.org/10.2478/acve-2019-0016
conv_524
Jezdimirović N, Kureljušić B, Ivetić V, Krnjaić D, Radanović O, Žutić J, Spalević L, Jovanović M. Comparative pathomorphological, mycological and molecular examination of turkey poults with different immunological status experimentally infected with aspergillus fumigatus. in Acta Veterinaria-Beograd. 2019;69(2):201-217.
doi:10.2478/acve-2019-0016
conv_524 .
Jezdimirović, Nemanja, Kureljušić, Branislav, Ivetić, Vojin, Krnjaić, Dejan, Radanović, Oliver, Žutić, Jadranka, Spalević, Ljiljana, Jovanović, Milijan, "Comparative pathomorphological, mycological and molecular examination of turkey poults with different immunological status experimentally infected with aspergillus fumigatus" in Acta Veterinaria-Beograd, 69, no. 2 (2019):201-217,
https://doi.org/10.2478/acve-2019-0016 .,
conv_524 .
1

The influence of Aspergillus fumigatus infection on some hematological parameters in turkey’s poults

Jezdimirović, Nemanja; Kureljušić, Branislav; Savić, Božidar; Kureljušić, Jasna; Cvetojević, Đorđe; Dimitrijević, Blagoje; Jakić-Dimić, Dobrila; Milićević, Vesna; Maslić-Strižak, Danka; Ivetić, Vojin; Jovanović, Milijan

(Novi Sad : Scientific Veterinary Institute "Novi Sad", 2015)

TY  - CONF
AU  - Jezdimirović, Nemanja
AU  - Kureljušić, Branislav
AU  - Savić, Božidar
AU  - Kureljušić, Jasna
AU  - Cvetojević, Đorđe
AU  - Dimitrijević, Blagoje
AU  - Jakić-Dimić, Dobrila
AU  - Milićević, Vesna
AU  - Maslić-Strižak, Danka
AU  - Ivetić, Vojin
AU  - Jovanović, Milijan
PY  - 2015
UR  - https://reponivs.nivs.rs/handle/123456789/528
AB  - Aspergillus fumigatus is a ubiquitous, saprophytic mould, but as a facultative pathogen,
in imunocompromise organism it can cause life-threatening conditions. Aspergillosis is
frequent fungal disease of young turkey. The hallmark of aspergillosis is granulomatous
inflammation of respiratory system with multiple yellow nodules, usually localized in
lungs and air sacs, although generalized form is possible as well. The aim of this study
was to investigate the effect of Aspergillus fumigatus infection in hematological
parameters in turkeys. Artificial infection was induced by intratracheal application of 0.3
ml of the spore suspension (1x107/ml) in 30 poults, 2 weeks old. The blood samples
were taken by puncture v. Brachialis, 0, 14 and 21 days after infection. The
determination of red blood cell, hematocrit, hemoglobin, white blood cell, heterophile,
lymphocyte and basophil was done using an Automated Hematology Analyzer
(Hemavet CDC Mascot, USA).The analysis of hematological results 14 days post
infection revealed significantly lower concentrations of hemoglobin (10.02 ± 0.56 vs
9.08 ± 0.41 g/dL; p <0.05) compared to the control group. In the same period, the
number of leukocytes was significantly higher (6.17 ± 0.96x109 vs 15.66 ± 4.14x109
cells/L; p <0.01). Within the lineage of white blood cells, number of heterophils,
basophils and lymphocytes were significantly lower compared to the control group (p
<0.01).The decreasing trend in the number of red cells was continued on day 21 after
infection in experimental group of turkeys (1.87 ± 0.07x1012; p <0.01) compared to the
control group. Hematocrit values were also significantly declined linearly as a function
of time (34.50 ± 1.87 vs. 31.70 ± 2.71 vs. 30.60 ± 1.17%). Leukocyte count was
statistically highly significant increasing during the period of infection, and the
maximum value (25.86 ± 8.67x109 cells/L) was found 21. day. Number of heterophile in
this period of infection was significantly lower (15.25 ± 5.26x109 cells/L) compared to
zero-day infection (47.83 ± 11.79x109 cells/L; p <0.001), and slightly increased
compared to the 14th day of infection (6.58 ± 2.32x109 cells / L; p <0.05). Number of
limfocita cells after 21 days of infection was significantly lower (9.87 ± 4.02x109 cells /
L) when compared to the day of infection (p <0.001), and slightly increased in
comparison with day 14 of infection (8.06±3.94x109 cells/L; p <0.05). After 21. days of
infection the number of basophils was significantly lower (0.74 ± 0.63x109 cells L)
when compared to the day of infection (6.00 ± 2.60x109 cells / L; p <0.001), and without
changes compared to the 14th day from infection.The impact of A. fumigatus on the
haematological parameters in turkeys showed anemia and a strong inflammatory
response. Further research is needed to define more closely changes within white blood
cells.
PB  - Novi Sad : Scientific Veterinary Institute "Novi Sad"
C3  - First International Symposium of Veterinary Medicine "One Health - New Challenges" (ISVM2015)
T1  - The influence of Aspergillus fumigatus infection on some hematological parameters in turkey’s poults
EP  - 36
SP  - 36
ER  - 
@conference{
author = "Jezdimirović, Nemanja and Kureljušić, Branislav and Savić, Božidar and Kureljušić, Jasna and Cvetojević, Đorđe and Dimitrijević, Blagoje and Jakić-Dimić, Dobrila and Milićević, Vesna and Maslić-Strižak, Danka and Ivetić, Vojin and Jovanović, Milijan",
year = "2015",
abstract = "Aspergillus fumigatus is a ubiquitous, saprophytic mould, but as a facultative pathogen,
in imunocompromise organism it can cause life-threatening conditions. Aspergillosis is
frequent fungal disease of young turkey. The hallmark of aspergillosis is granulomatous
inflammation of respiratory system with multiple yellow nodules, usually localized in
lungs and air sacs, although generalized form is possible as well. The aim of this study
was to investigate the effect of Aspergillus fumigatus infection in hematological
parameters in turkeys. Artificial infection was induced by intratracheal application of 0.3
ml of the spore suspension (1x107/ml) in 30 poults, 2 weeks old. The blood samples
were taken by puncture v. Brachialis, 0, 14 and 21 days after infection. The
determination of red blood cell, hematocrit, hemoglobin, white blood cell, heterophile,
lymphocyte and basophil was done using an Automated Hematology Analyzer
(Hemavet CDC Mascot, USA).The analysis of hematological results 14 days post
infection revealed significantly lower concentrations of hemoglobin (10.02 ± 0.56 vs
9.08 ± 0.41 g/dL; p <0.05) compared to the control group. In the same period, the
number of leukocytes was significantly higher (6.17 ± 0.96x109 vs 15.66 ± 4.14x109
cells/L; p <0.01). Within the lineage of white blood cells, number of heterophils,
basophils and lymphocytes were significantly lower compared to the control group (p
<0.01).The decreasing trend in the number of red cells was continued on day 21 after
infection in experimental group of turkeys (1.87 ± 0.07x1012; p <0.01) compared to the
control group. Hematocrit values were also significantly declined linearly as a function
of time (34.50 ± 1.87 vs. 31.70 ± 2.71 vs. 30.60 ± 1.17%). Leukocyte count was
statistically highly significant increasing during the period of infection, and the
maximum value (25.86 ± 8.67x109 cells/L) was found 21. day. Number of heterophile in
this period of infection was significantly lower (15.25 ± 5.26x109 cells/L) compared to
zero-day infection (47.83 ± 11.79x109 cells/L; p <0.001), and slightly increased
compared to the 14th day of infection (6.58 ± 2.32x109 cells / L; p <0.05). Number of
limfocita cells after 21 days of infection was significantly lower (9.87 ± 4.02x109 cells /
L) when compared to the day of infection (p <0.001), and slightly increased in
comparison with day 14 of infection (8.06±3.94x109 cells/L; p <0.05). After 21. days of
infection the number of basophils was significantly lower (0.74 ± 0.63x109 cells L)
when compared to the day of infection (6.00 ± 2.60x109 cells / L; p <0.001), and without
changes compared to the 14th day from infection.The impact of A. fumigatus on the
haematological parameters in turkeys showed anemia and a strong inflammatory
response. Further research is needed to define more closely changes within white blood
cells.",
publisher = "Novi Sad : Scientific Veterinary Institute "Novi Sad"",
journal = "First International Symposium of Veterinary Medicine "One Health - New Challenges" (ISVM2015)",
title = "The influence of Aspergillus fumigatus infection on some hematological parameters in turkey’s poults",
pages = "36-36"
}
Jezdimirović, N., Kureljušić, B., Savić, B., Kureljušić, J., Cvetojević, Đ., Dimitrijević, B., Jakić-Dimić, D., Milićević, V., Maslić-Strižak, D., Ivetić, V.,& Jovanović, M.. (2015). The influence of Aspergillus fumigatus infection on some hematological parameters in turkey’s poults. in First International Symposium of Veterinary Medicine "One Health - New Challenges" (ISVM2015)
Novi Sad : Scientific Veterinary Institute "Novi Sad"., 36-36.
Jezdimirović N, Kureljušić B, Savić B, Kureljušić J, Cvetojević Đ, Dimitrijević B, Jakić-Dimić D, Milićević V, Maslić-Strižak D, Ivetić V, Jovanović M. The influence of Aspergillus fumigatus infection on some hematological parameters in turkey’s poults. in First International Symposium of Veterinary Medicine "One Health - New Challenges" (ISVM2015). 2015;:36-36..
Jezdimirović, Nemanja, Kureljušić, Branislav, Savić, Božidar, Kureljušić, Jasna, Cvetojević, Đorđe, Dimitrijević, Blagoje, Jakić-Dimić, Dobrila, Milićević, Vesna, Maslić-Strižak, Danka, Ivetić, Vojin, Jovanović, Milijan, "The influence of Aspergillus fumigatus infection on some hematological parameters in turkey’s poults" in First International Symposium of Veterinary Medicine "One Health - New Challenges" (ISVM2015) (2015):36-36.

Melamine-induced nephrotoxicity in weaned piglets in Serbia

Kureljušić, Branislav; Ivetić, Vojin; Savić, Božidar; Kureljušić, Jasna; Jezdimirović, Nemanja; Cvetojević, Đorđe; Vesković-Moračanin, Slavica; Stefanović, Srđan; Juntes, Polona; Jakić-Dimić, Dobrila

(University of Ljubljana - Veterinary Faculty, 2014)

TY  - JOUR
AU  - Kureljušić, Branislav
AU  - Ivetić, Vojin
AU  - Savić, Božidar
AU  - Kureljušić, Jasna
AU  - Jezdimirović, Nemanja
AU  - Cvetojević, Đorđe
AU  - Vesković-Moračanin, Slavica
AU  - Stefanović, Srđan
AU  - Juntes, Polona
AU  - Jakić-Dimić, Dobrila
PY  - 2014
UR  - https://reponivs.nivs.rs/handle/123456789/281
AB  - Melamine is not approved for use in animal feed, although evidence of melamine poisoning in pigs has been found. Melamine in animal feed can induce nephrolithiasis, chronic kidney inflammation, bladder carcinoma and even death in animals. In September 2011, nine hundred 40-60-day-old piglets from a commercial finishing farm in Serbia developed anorexia, wasting, polydipsia and lethargy. At necropsy, the main macroscopic finding in ten necropsied piglets was observed on the kidneys. The kidneys were slightly enlarged and firm in consistency. The cortical surface was wrinkled, with a diffuse distribution of the yellow foci in the cortex and medulla. Microscopically, these yellow foci were accumulations of crystals located predominantly within the lumina of the dilated distal straight and convoluted tubules and collecting ducts. The crystals appeared variable and birefringent when viewed under polarized light. Two types of crystals were observed: the first type had several dark striations radiating from the eosinophilic round centre, while the second type consisted of pale green to brown irregular striated aggregates without an eosinophilic centre. In all cases, multifocally, there were moderate aggregates of lymphocytes, plasma cells, macrophages and multinucleated giant cells within the interstitium. From the clinical signs, necropsy findings, histochemical and toxicological results, it was concluded that melamine-contaminated feed was the primary cause of nephrotoxicity and death in the observed piglets. To our knowledge, this is the first report of melamine poisoning in pigs in Serbia.
PB  - University of Ljubljana - Veterinary Faculty
T2  - Slovenian Veterinary Research
T1  - Melamine-induced nephrotoxicity in weaned piglets in Serbia
EP  - 146
IS  - 3
SP  - 141
VL  - 51
UR  - conv_669
ER  - 
@article{
author = "Kureljušić, Branislav and Ivetić, Vojin and Savić, Božidar and Kureljušić, Jasna and Jezdimirović, Nemanja and Cvetojević, Đorđe and Vesković-Moračanin, Slavica and Stefanović, Srđan and Juntes, Polona and Jakić-Dimić, Dobrila",
year = "2014",
abstract = "Melamine is not approved for use in animal feed, although evidence of melamine poisoning in pigs has been found. Melamine in animal feed can induce nephrolithiasis, chronic kidney inflammation, bladder carcinoma and even death in animals. In September 2011, nine hundred 40-60-day-old piglets from a commercial finishing farm in Serbia developed anorexia, wasting, polydipsia and lethargy. At necropsy, the main macroscopic finding in ten necropsied piglets was observed on the kidneys. The kidneys were slightly enlarged and firm in consistency. The cortical surface was wrinkled, with a diffuse distribution of the yellow foci in the cortex and medulla. Microscopically, these yellow foci were accumulations of crystals located predominantly within the lumina of the dilated distal straight and convoluted tubules and collecting ducts. The crystals appeared variable and birefringent when viewed under polarized light. Two types of crystals were observed: the first type had several dark striations radiating from the eosinophilic round centre, while the second type consisted of pale green to brown irregular striated aggregates without an eosinophilic centre. In all cases, multifocally, there were moderate aggregates of lymphocytes, plasma cells, macrophages and multinucleated giant cells within the interstitium. From the clinical signs, necropsy findings, histochemical and toxicological results, it was concluded that melamine-contaminated feed was the primary cause of nephrotoxicity and death in the observed piglets. To our knowledge, this is the first report of melamine poisoning in pigs in Serbia.",
publisher = "University of Ljubljana - Veterinary Faculty",
journal = "Slovenian Veterinary Research",
title = "Melamine-induced nephrotoxicity in weaned piglets in Serbia",
pages = "146-141",
number = "3",
volume = "51",
url = "conv_669"
}
Kureljušić, B., Ivetić, V., Savić, B., Kureljušić, J., Jezdimirović, N., Cvetojević, Đ., Vesković-Moračanin, S., Stefanović, S., Juntes, P.,& Jakić-Dimić, D.. (2014). Melamine-induced nephrotoxicity in weaned piglets in Serbia. in Slovenian Veterinary Research
University of Ljubljana - Veterinary Faculty., 51(3), 141-146.
conv_669
Kureljušić B, Ivetić V, Savić B, Kureljušić J, Jezdimirović N, Cvetojević Đ, Vesković-Moračanin S, Stefanović S, Juntes P, Jakić-Dimić D. Melamine-induced nephrotoxicity in weaned piglets in Serbia. in Slovenian Veterinary Research. 2014;51(3):141-146.
conv_669 .
Kureljušić, Branislav, Ivetić, Vojin, Savić, Božidar, Kureljušić, Jasna, Jezdimirović, Nemanja, Cvetojević, Đorđe, Vesković-Moračanin, Slavica, Stefanović, Srđan, Juntes, Polona, Jakić-Dimić, Dobrila, "Melamine-induced nephrotoxicity in weaned piglets in Serbia" in Slovenian Veterinary Research, 51, no. 3 (2014):141-146,
conv_669 .

Hemoragično-nekrotični enteritis kod roditelja brojlera

Jezdimirović, Nemanja; Kureljušić, Branislav; Ivetić, Vojin; Kureljušić, Jasna; Cvetojević, Đ.; Maslić-Strižak, Danka; Radanović, Oliver; Jezdimirović, Milanka

(Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd, 2014)

TY  - JOUR
AU  - Jezdimirović, Nemanja
AU  - Kureljušić, Branislav
AU  - Ivetić, Vojin
AU  - Kureljušić, Jasna
AU  - Cvetojević, Đ.
AU  - Maslić-Strižak, Danka
AU  - Radanović, Oliver
AU  - Jezdimirović, Milanka
PY  - 2014
UR  - https://reponivs.nivs.rs/handle/123456789/260
AB  - Ispitivanja su imala za cilj da se utvrdi uticaj Clostridium perfringens tip A na razvoj patomorfološkog supstrata, njegov intenzitet i distribuciju kod teških roditelja uzrasta petnaest nedelja. Ispitivanje je obavljeno na leševima 8 koka i 7 petlova teških roditelja provenijencije COBB 500. Posle izvršene obdukcije, za histopatološko ispitivanje uzeti su uzorci promenjenih delova jejunuma i jetre, a za bakteriološko i molekularno ispitivanje crevni sadržaj jejunuma. Kod svih leševa u otvorenoj pleuroperitonealnoj šupljini, već in situ, uočava se promenjeni deo jejunuma. On je bio izrazito dilatiran celom dužinom, a njegov zid plavičasto-sive boje sa diseminiranim subseroznim punktiformnim krvnim ekstravazatima. Njegovim otvaranjem iz lumena je izlazio polutečni sadržaj u kojem plivaju krvni koagulumi i krpičasti delovi nekrotične mukoze. Mikroskopskim pregledom isečaka tkiva tankih creva bojenih HE metodom zapažena je difuzna nekroza crevnih resica. One su destruisane i zamenjene eozinofilnom bestrukturnom masom. Pored toga, uočava se edem submukoze, kongestija kapilara i krvni ekstravazati u mukozi, kao i infiltracija neutrofilnih granulocita u lamini propriji. Navedene mikroskopske alteracije oslikavaju hemoragično-nekrotični enteritis. Mikroskopskim pregledom isečka tankih creva bojenih Brown & Brenn metodom ustanovljene su kolonije bakterija u distalnim delovima submukoze. Bakteriološkim ispitivanjem u anaerobnim uslovima, izolovana kultura je identifikovana kao Clostridium perfringens. Primenom multiplex PCR dobijeni izolat je genotipiziran kao Clostridium perfringens tip A. Na osnovu patomorfoloških, bakterioloških i molekularnih ispitivanja može da se zaključi da infekcija teških roditelja Clostridium perfringens tipa A protiče sa slikom difuznog hemoragično-nekrotičnog jejunitisa, da uzročnik duboko penetrira u tkivo jejunuma i da su pšenica i pšenične mekinje činili favorizujući faktor za proliferaciju etiološkog agensa.
AB  - The aim of the investigation was to determine the influence of Clostridium perfringens type A on the development of pathomorphological substrate, its intensity and distribution in fifteen weeks old heavy breeds broilers. The investigation was carried out on corpses of 8 hens and 7 roosters of heavy breeds of provenance COBB 500. After the completion of the autopsy, samples of altered parts of jejunum and liver were taken for histopathological examination, and jejunum intestinal contents for bacteriological examination. In all the corpses, in open pleuroperitoneal cavity, even in situ, an altered part of jejunum can be noticed. It was extremely dilated the entire length, and its wall was bluish-gray with disseminated subserous punctiform blood extravasates. When opened, semi-liquid content with blood coagulums and patches of necrotic mucosa went out of it. By microscopic examination of small intestine tissue cuttings, colored by HE method, there was observed a diffuse necrosis of intestinal villi. They were destroyed and replaced by eosinophilic structureless mass. Furthermore, there could be noticed submucose oedema, capillary congestion and blood extravasates in mucosa, as well as infiltration of neutrophilic granulocytes in lamina propria. These microscopic alterations reflect hemorrhagic necrotic enteritis. By microscopic examining of small intestine cuttings colored according to Brown & Brenn method, colonies of bacteria in distal parts of the submucosa were found out. Using bacteriological tests in anaerobic conditions, there was isolated a culture identified as Clostridium perfringens. After applying of multiplex PCR, the obtained isolate was genotyped as Clostridium perfringens type A. On the basis of pathomorphological, bacteriological and molecular examinations, it can be concluded that the infection of heavy breeds with Clostridium perfringens type A is manifested by appearance of haemorrhagic-necrotic jejunitis, that the causer penetrates deeply into jejunum tissue and that wheat and wheat bran were a favoring factor for proliferation of the etiological agent.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski glasnik
T1  - Hemoragično-nekrotični enteritis kod roditelja brojlera
T1  - Gemorragičeski-nekrotičeskij ënterit u roditel'skogo stada tjaželyh porod
T1  - Haemorrhagic-necrotic enteritis in heavy breeds broilers
EP  - 273
IS  - 3-4
SP  - 265
VL  - 68
DO  - 10.2298/VETGL1404265J
UR  - conv_102
ER  - 
@article{
author = "Jezdimirović, Nemanja and Kureljušić, Branislav and Ivetić, Vojin and Kureljušić, Jasna and Cvetojević, Đ. and Maslić-Strižak, Danka and Radanović, Oliver and Jezdimirović, Milanka",
year = "2014",
abstract = "Ispitivanja su imala za cilj da se utvrdi uticaj Clostridium perfringens tip A na razvoj patomorfološkog supstrata, njegov intenzitet i distribuciju kod teških roditelja uzrasta petnaest nedelja. Ispitivanje je obavljeno na leševima 8 koka i 7 petlova teških roditelja provenijencije COBB 500. Posle izvršene obdukcije, za histopatološko ispitivanje uzeti su uzorci promenjenih delova jejunuma i jetre, a za bakteriološko i molekularno ispitivanje crevni sadržaj jejunuma. Kod svih leševa u otvorenoj pleuroperitonealnoj šupljini, već in situ, uočava se promenjeni deo jejunuma. On je bio izrazito dilatiran celom dužinom, a njegov zid plavičasto-sive boje sa diseminiranim subseroznim punktiformnim krvnim ekstravazatima. Njegovim otvaranjem iz lumena je izlazio polutečni sadržaj u kojem plivaju krvni koagulumi i krpičasti delovi nekrotične mukoze. Mikroskopskim pregledom isečaka tkiva tankih creva bojenih HE metodom zapažena je difuzna nekroza crevnih resica. One su destruisane i zamenjene eozinofilnom bestrukturnom masom. Pored toga, uočava se edem submukoze, kongestija kapilara i krvni ekstravazati u mukozi, kao i infiltracija neutrofilnih granulocita u lamini propriji. Navedene mikroskopske alteracije oslikavaju hemoragično-nekrotični enteritis. Mikroskopskim pregledom isečka tankih creva bojenih Brown & Brenn metodom ustanovljene su kolonije bakterija u distalnim delovima submukoze. Bakteriološkim ispitivanjem u anaerobnim uslovima, izolovana kultura je identifikovana kao Clostridium perfringens. Primenom multiplex PCR dobijeni izolat je genotipiziran kao Clostridium perfringens tip A. Na osnovu patomorfoloških, bakterioloških i molekularnih ispitivanja može da se zaključi da infekcija teških roditelja Clostridium perfringens tipa A protiče sa slikom difuznog hemoragično-nekrotičnog jejunitisa, da uzročnik duboko penetrira u tkivo jejunuma i da su pšenica i pšenične mekinje činili favorizujući faktor za proliferaciju etiološkog agensa., The aim of the investigation was to determine the influence of Clostridium perfringens type A on the development of pathomorphological substrate, its intensity and distribution in fifteen weeks old heavy breeds broilers. The investigation was carried out on corpses of 8 hens and 7 roosters of heavy breeds of provenance COBB 500. After the completion of the autopsy, samples of altered parts of jejunum and liver were taken for histopathological examination, and jejunum intestinal contents for bacteriological examination. In all the corpses, in open pleuroperitoneal cavity, even in situ, an altered part of jejunum can be noticed. It was extremely dilated the entire length, and its wall was bluish-gray with disseminated subserous punctiform blood extravasates. When opened, semi-liquid content with blood coagulums and patches of necrotic mucosa went out of it. By microscopic examination of small intestine tissue cuttings, colored by HE method, there was observed a diffuse necrosis of intestinal villi. They were destroyed and replaced by eosinophilic structureless mass. Furthermore, there could be noticed submucose oedema, capillary congestion and blood extravasates in mucosa, as well as infiltration of neutrophilic granulocytes in lamina propria. These microscopic alterations reflect hemorrhagic necrotic enteritis. By microscopic examining of small intestine cuttings colored according to Brown & Brenn method, colonies of bacteria in distal parts of the submucosa were found out. Using bacteriological tests in anaerobic conditions, there was isolated a culture identified as Clostridium perfringens. After applying of multiplex PCR, the obtained isolate was genotyped as Clostridium perfringens type A. On the basis of pathomorphological, bacteriological and molecular examinations, it can be concluded that the infection of heavy breeds with Clostridium perfringens type A is manifested by appearance of haemorrhagic-necrotic jejunitis, that the causer penetrates deeply into jejunum tissue and that wheat and wheat bran were a favoring factor for proliferation of the etiological agent.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski glasnik",
title = "Hemoragično-nekrotični enteritis kod roditelja brojlera, Gemorragičeski-nekrotičeskij ënterit u roditel'skogo stada tjaželyh porod, Haemorrhagic-necrotic enteritis in heavy breeds broilers",
pages = "273-265",
number = "3-4",
volume = "68",
doi = "10.2298/VETGL1404265J",
url = "conv_102"
}
Jezdimirović, N., Kureljušić, B., Ivetić, V., Kureljušić, J., Cvetojević, Đ., Maslić-Strižak, D., Radanović, O.,& Jezdimirović, M.. (2014). Hemoragično-nekrotični enteritis kod roditelja brojlera. in Veterinarski glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 68(3-4), 265-273.
https://doi.org/10.2298/VETGL1404265J
conv_102
Jezdimirović N, Kureljušić B, Ivetić V, Kureljušić J, Cvetojević Đ, Maslić-Strižak D, Radanović O, Jezdimirović M. Hemoragično-nekrotični enteritis kod roditelja brojlera. in Veterinarski glasnik. 2014;68(3-4):265-273.
doi:10.2298/VETGL1404265J
conv_102 .
Jezdimirović, Nemanja, Kureljušić, Branislav, Ivetić, Vojin, Kureljušić, Jasna, Cvetojević, Đ., Maslić-Strižak, Danka, Radanović, Oliver, Jezdimirović, Milanka, "Hemoragično-nekrotični enteritis kod roditelja brojlera" in Veterinarski glasnik, 68, no. 3-4 (2014):265-273,
https://doi.org/10.2298/VETGL1404265J .,
conv_102 .

Ispitivanje patomorfoloških karakteristika ulkusa abomazusa kod visoko-mlečnih krava

Kureljušić, Branislav; Ivetić, Vojin; Savić, Božidar; Jezdimirović, Nemanja; Cvetojević, Đ.; Kureljušić, Jasna; Ilić, Živka; Stanojević, Slobodan; Stevančević, M.

(Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd, 2013)

TY  - JOUR
AU  - Kureljušić, Branislav
AU  - Ivetić, Vojin
AU  - Savić, Božidar
AU  - Jezdimirović, Nemanja
AU  - Cvetojević, Đ.
AU  - Kureljušić, Jasna
AU  - Ilić, Živka
AU  - Stanojević, Slobodan
AU  - Stevančević, M.
PY  - 2013
UR  - https://reponivs.nivs.rs/handle/123456789/223
AB  - Ulkus abomazusa predstavlja lokalni proces autodigestije sluznice koja nastaje pod uticajem acidopeptičke aktivnosti ili je proizvod patofizioloških mehanizama pri kojima sluznica ne može da se suprotstavi destruktivnom dejstvu kiselog želudačnog soka, odnosno kada je narušena ravnoteža između protektivnih i agresivnih faktora. U poslednje vreme sve je više podataka u literaturi o pojavi ulkusa abomazusa progredijentnog toka kod visoko mlečnih krava. Cilj ovog rada je bio da se ispita morfologija, lokalizacija i tipizacija ulkusa abomazusa kod visokomlečnih krava, kao doprinos razjašnjenju ulcerogeneze. U ovom radu je ispitano deset visoko-mlečnih krava koje su uginule u ranom puerperijumu i dve visokogravidne junice holštajn rase iz uvoza koje su uginule deset dana posle transporta, a poticale su sa farmi mlečnih goveda iz okoline Beograda. Nakon uginuća, izvršenaje obdukcija i uzorci alterisanog tkiva abomazusa su uzorkovani za histopatološka i mikološka ispitivanja. Ustanovljene ulceracije abomazusa kod deset obdukovanih životinja međusobno su se razlikovale kako po lokalizaciji i morfologiji tako i po stepenu zidne penetracije što ih diferencira u četiri tipa. Ulkusi tipa jedan i dva ustanovljeni su kod dve junice i šest krava, bili su multipli, situirani u korpusnoj i pilorusnoj regiji, jedan do drugog i većinom okruglog, ovalnog ili potpuno nepravilnog oblika i penetrirali su do submukoze ili dublje u mišićni sloj. Ulceracije tipa 1 i 2 ustanovljene su i kod jedne krave sa limfomom. Ulceracije su bile multiple i uglavnom ovalnog oblika, a smeštene u korpusu i pilorusu abomazusa u nizu. Perforirajući ulkus tipa tri sa cirkumskriptnim peritonitisom ustanovljen je kod jedne krave. Ulkus je prečnika3 mm i nalazi se u korpusu abomazusa bliže kurvaturi major. Interesantan je nalaz simultane pojave perforirajućeg ulkusa tipa četiri i ulkusa tipa jedan koji su locirani jedan do drugog i dimenzija 7X4 cm. Histopatološkim ispitivanjem uzoraka abomazusa sa ulceracijama bojenih hematoksilin-eozin metodom ustanovljena je koagulaciona nekroza u području ulceracije i umereni zapaljenski infiltrat u lamini propriji. Kod krave sa limfomom, je pored ovog ustanovljen i obilan tumorozni limfoidni proliferat. Mikotični ulcerozni abomazitis ustanovljen je kod jedne krave sedam dana posle partusa. Ulceracije su multiple, okruglog oblika, ponekad konfluiraju, a zastupljene su na korpusnom i pilorusnom delu abomazusa. Iz ovih lezija izolovane su gljivice Aspergillus fumigatus i Mucorspp. Histopatološkom analizom, u uzorcima tkiva abomazusa obojenih Grocott metodom, dokazane su septirane i neseptirane hife gljivica u krvnim sudovima i okolnom tkivu tunike mukoze i submukoze. Ulkusi abomazusa kod ispitanih krava se najčešće javljaju na korpusnoj i pilorusnoj regiji, većinom su okrugli ili ovalni, a zastupljeni su sa sva četiri tipa. Uzrok njihovog nastanka može se vezati za stresogene faktore (peripartalni period, transport), ishranu, vazoaktivne gljivice, prisustvo geosedimenta i limfom abomazusa.
AB  - The aim of this paper was to investigate the morphology, localization and typization of abomasal ulcers in high-yielding dairy cows, as a contribution to the existing knowledge on the subject. A total of ten high-yielding dairy cows which died during the period of early puerperium and two Holstein heifers which died in late pregnancy after transport to a new location were investigated in this study. Samples of altered abomasal tissue were taken at necropsy for further histopathology and mycology investigations. The disclosed ulcerations of the abomasums in twelve investigated animals differed among them not only as far as localization and morphology are concerned, but also in the degree of intramural penetration. Such differences distinguished them into four types. Ulcerations of type one and type two, recorded in two heifers and six cows, as well as in one cow with lymphoma of the abomasum. Perforating ulcer of type three, with circumscripted peritonitis was diagnosed in one cow in the corpus of the abomasum close to the curvatura major. An interesting finding was the simultaneous occurrence of type four perforating ulcer and ulcer-type one, which were located next to each other. Histopathological examination of the structure of the ulcerated abomasum dyed with the hematoxylin-eosin method showed that coagulation necrosis in the area of the ulceration revealed a moderate inflammatory infiltrate in the lamina propria. In the cow affected with lymphoma, a copious tumorous lymphoid infiltrate was concurrent. Mycotic ulcerative abomasitis was described in a cow seven days after calving. In these lesions Aspergillus fumigatus and Mucor spp. were isolated. Histopathological analysis of tissue samples dyed with the Grocott method, described septed and nonsepted hyphae in the blood vessels and surrounding tissue of the tunica mucosa and submucosa.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Ispitivanje patomorfoloških karakteristika ulkusa abomazusa kod visoko-mlečnih krava
T1  - Pathomorphological characteristics of abomasal ulcers in high-yielding dairy cows
EP  - 246
IS  - 2-3
SP  - 237
VL  - 63
DO  - 10.2298/AVB1303237K
UR  - conv_417
ER  - 
@article{
author = "Kureljušić, Branislav and Ivetić, Vojin and Savić, Božidar and Jezdimirović, Nemanja and Cvetojević, Đ. and Kureljušić, Jasna and Ilić, Živka and Stanojević, Slobodan and Stevančević, M.",
year = "2013",
abstract = "Ulkus abomazusa predstavlja lokalni proces autodigestije sluznice koja nastaje pod uticajem acidopeptičke aktivnosti ili je proizvod patofizioloških mehanizama pri kojima sluznica ne može da se suprotstavi destruktivnom dejstvu kiselog želudačnog soka, odnosno kada je narušena ravnoteža između protektivnih i agresivnih faktora. U poslednje vreme sve je više podataka u literaturi o pojavi ulkusa abomazusa progredijentnog toka kod visoko mlečnih krava. Cilj ovog rada je bio da se ispita morfologija, lokalizacija i tipizacija ulkusa abomazusa kod visokomlečnih krava, kao doprinos razjašnjenju ulcerogeneze. U ovom radu je ispitano deset visoko-mlečnih krava koje su uginule u ranom puerperijumu i dve visokogravidne junice holštajn rase iz uvoza koje su uginule deset dana posle transporta, a poticale su sa farmi mlečnih goveda iz okoline Beograda. Nakon uginuća, izvršenaje obdukcija i uzorci alterisanog tkiva abomazusa su uzorkovani za histopatološka i mikološka ispitivanja. Ustanovljene ulceracije abomazusa kod deset obdukovanih životinja međusobno su se razlikovale kako po lokalizaciji i morfologiji tako i po stepenu zidne penetracije što ih diferencira u četiri tipa. Ulkusi tipa jedan i dva ustanovljeni su kod dve junice i šest krava, bili su multipli, situirani u korpusnoj i pilorusnoj regiji, jedan do drugog i većinom okruglog, ovalnog ili potpuno nepravilnog oblika i penetrirali su do submukoze ili dublje u mišićni sloj. Ulceracije tipa 1 i 2 ustanovljene su i kod jedne krave sa limfomom. Ulceracije su bile multiple i uglavnom ovalnog oblika, a smeštene u korpusu i pilorusu abomazusa u nizu. Perforirajući ulkus tipa tri sa cirkumskriptnim peritonitisom ustanovljen je kod jedne krave. Ulkus je prečnika3 mm i nalazi se u korpusu abomazusa bliže kurvaturi major. Interesantan je nalaz simultane pojave perforirajućeg ulkusa tipa četiri i ulkusa tipa jedan koji su locirani jedan do drugog i dimenzija 7X4 cm. Histopatološkim ispitivanjem uzoraka abomazusa sa ulceracijama bojenih hematoksilin-eozin metodom ustanovljena je koagulaciona nekroza u području ulceracije i umereni zapaljenski infiltrat u lamini propriji. Kod krave sa limfomom, je pored ovog ustanovljen i obilan tumorozni limfoidni proliferat. Mikotični ulcerozni abomazitis ustanovljen je kod jedne krave sedam dana posle partusa. Ulceracije su multiple, okruglog oblika, ponekad konfluiraju, a zastupljene su na korpusnom i pilorusnom delu abomazusa. Iz ovih lezija izolovane su gljivice Aspergillus fumigatus i Mucorspp. Histopatološkom analizom, u uzorcima tkiva abomazusa obojenih Grocott metodom, dokazane su septirane i neseptirane hife gljivica u krvnim sudovima i okolnom tkivu tunike mukoze i submukoze. Ulkusi abomazusa kod ispitanih krava se najčešće javljaju na korpusnoj i pilorusnoj regiji, većinom su okrugli ili ovalni, a zastupljeni su sa sva četiri tipa. Uzrok njihovog nastanka može se vezati za stresogene faktore (peripartalni period, transport), ishranu, vazoaktivne gljivice, prisustvo geosedimenta i limfom abomazusa., The aim of this paper was to investigate the morphology, localization and typization of abomasal ulcers in high-yielding dairy cows, as a contribution to the existing knowledge on the subject. A total of ten high-yielding dairy cows which died during the period of early puerperium and two Holstein heifers which died in late pregnancy after transport to a new location were investigated in this study. Samples of altered abomasal tissue were taken at necropsy for further histopathology and mycology investigations. The disclosed ulcerations of the abomasums in twelve investigated animals differed among them not only as far as localization and morphology are concerned, but also in the degree of intramural penetration. Such differences distinguished them into four types. Ulcerations of type one and type two, recorded in two heifers and six cows, as well as in one cow with lymphoma of the abomasum. Perforating ulcer of type three, with circumscripted peritonitis was diagnosed in one cow in the corpus of the abomasum close to the curvatura major. An interesting finding was the simultaneous occurrence of type four perforating ulcer and ulcer-type one, which were located next to each other. Histopathological examination of the structure of the ulcerated abomasum dyed with the hematoxylin-eosin method showed that coagulation necrosis in the area of the ulceration revealed a moderate inflammatory infiltrate in the lamina propria. In the cow affected with lymphoma, a copious tumorous lymphoid infiltrate was concurrent. Mycotic ulcerative abomasitis was described in a cow seven days after calving. In these lesions Aspergillus fumigatus and Mucor spp. were isolated. Histopathological analysis of tissue samples dyed with the Grocott method, described septed and nonsepted hyphae in the blood vessels and surrounding tissue of the tunica mucosa and submucosa.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Ispitivanje patomorfoloških karakteristika ulkusa abomazusa kod visoko-mlečnih krava, Pathomorphological characteristics of abomasal ulcers in high-yielding dairy cows",
pages = "246-237",
number = "2-3",
volume = "63",
doi = "10.2298/AVB1303237K",
url = "conv_417"
}
Kureljušić, B., Ivetić, V., Savić, B., Jezdimirović, N., Cvetojević, Đ., Kureljušić, J., Ilić, Ž., Stanojević, S.,& Stevančević, M.. (2013). Ispitivanje patomorfoloških karakteristika ulkusa abomazusa kod visoko-mlečnih krava. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 63(2-3), 237-246.
https://doi.org/10.2298/AVB1303237K
conv_417
Kureljušić B, Ivetić V, Savić B, Jezdimirović N, Cvetojević Đ, Kureljušić J, Ilić Ž, Stanojević S, Stevančević M. Ispitivanje patomorfoloških karakteristika ulkusa abomazusa kod visoko-mlečnih krava. in Acta Veterinaria-Beograd. 2013;63(2-3):237-246.
doi:10.2298/AVB1303237K
conv_417 .
Kureljušić, Branislav, Ivetić, Vojin, Savić, Božidar, Jezdimirović, Nemanja, Cvetojević, Đ., Kureljušić, Jasna, Ilić, Živka, Stanojević, Slobodan, Stevančević, M., "Ispitivanje patomorfoloških karakteristika ulkusa abomazusa kod visoko-mlečnih krava" in Acta Veterinaria-Beograd, 63, no. 2-3 (2013):237-246,
https://doi.org/10.2298/AVB1303237K .,
conv_417 .
5
2
5

Melamine-induced nephrotoxicity in weaned pigs

Kureljušić, Branislav; Ivetić, Vojin; Savić, Božidar; Krnjajić, S.; Ristevski, D.; Kureljušić, Jasna; Jezdimirović, Nemanja; Jakić-Dimić, Dobrila

(Elsevier Sci Ltd, Oxford, 2013)

TY  - CONF
AU  - Kureljušić, Branislav
AU  - Ivetić, Vojin
AU  - Savić, Božidar
AU  - Krnjajić, S.
AU  - Ristevski, D.
AU  - Kureljušić, Jasna
AU  - Jezdimirović, Nemanja
AU  - Jakić-Dimić, Dobrila
PY  - 2013
UR  - https://reponivs.nivs.rs/handle/123456789/221
PB  - Elsevier Sci Ltd, Oxford
C3  - Journal of Comparative Pathology
T1  - Melamine-induced nephrotoxicity in weaned pigs
EP  - 94
IS  - 1
SP  - 94
VL  - 148
DO  - 10.1016/j.jcpa.2012.11.193
UR  - conv_412
ER  - 
@conference{
author = "Kureljušić, Branislav and Ivetić, Vojin and Savić, Božidar and Krnjajić, S. and Ristevski, D. and Kureljušić, Jasna and Jezdimirović, Nemanja and Jakić-Dimić, Dobrila",
year = "2013",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Journal of Comparative Pathology",
title = "Melamine-induced nephrotoxicity in weaned pigs",
pages = "94-94",
number = "1",
volume = "148",
doi = "10.1016/j.jcpa.2012.11.193",
url = "conv_412"
}
Kureljušić, B., Ivetić, V., Savić, B., Krnjajić, S., Ristevski, D., Kureljušić, J., Jezdimirović, N.,& Jakić-Dimić, D.. (2013). Melamine-induced nephrotoxicity in weaned pigs. in Journal of Comparative Pathology
Elsevier Sci Ltd, Oxford., 148(1), 94-94.
https://doi.org/10.1016/j.jcpa.2012.11.193
conv_412
Kureljušić B, Ivetić V, Savić B, Krnjajić S, Ristevski D, Kureljušić J, Jezdimirović N, Jakić-Dimić D. Melamine-induced nephrotoxicity in weaned pigs. in Journal of Comparative Pathology. 2013;148(1):94-94.
doi:10.1016/j.jcpa.2012.11.193
conv_412 .
Kureljušić, Branislav, Ivetić, Vojin, Savić, Božidar, Krnjajić, S., Ristevski, D., Kureljušić, Jasna, Jezdimirović, Nemanja, Jakić-Dimić, Dobrila, "Melamine-induced nephrotoxicity in weaned pigs" in Journal of Comparative Pathology, 148, no. 1 (2013):94-94,
https://doi.org/10.1016/j.jcpa.2012.11.193 .,
conv_412 .

A Case Report: Isolation of Alysiella filiformis from Pig's Lungs

Žutić, Jadranka; Radosavljević, Vladimir; Radanović, Oliver; Ivetić, Vojin; Pavlović, Ivan; Žutić, Milenko

(Kafkas Univ, Veteriner Fakultesi Dergisi, Kars, 2013)

TY  - JOUR
AU  - Žutić, Jadranka
AU  - Radosavljević, Vladimir
AU  - Radanović, Oliver
AU  - Ivetić, Vojin
AU  - Pavlović, Ivan
AU  - Žutić, Milenko
PY  - 2013
UR  - https://reponivs.nivs.rs/handle/123456789/246
AB  - Alysiella filiformis is considered a common resident in the oral cavities of many animals. All reports of Alysiella indicate that it is restricted to the oral cavity of warm-blooded vertebrates, where it apparently is nonpathogenic. However, increased losses of young pigs occured in one farm in Serbia. Spumous content in bronchia and partly clotted blood in blood vesels of the lungs were present. Characteristic signs of oedema disease were present and E. coli serogroup 0139 was isolated. Furthermore, Alysiella filiformis was the single agent isolated from the lungs of diseased pigs. This is the first isolation of Alysiella filiformis from pig lungs.
PB  - Kafkas Univ, Veteriner Fakultesi Dergisi, Kars
T2  - Kafkas Universitesi Veteriner Fakultesi Dergisi
T1  - A Case Report: Isolation of Alysiella filiformis from Pig's Lungs
EP  - 723
IS  - 4
SP  - 721
VL  - 19
DO  - 10.9775/kvfd.2013.8648
UR  - conv_418
ER  - 
@article{
author = "Žutić, Jadranka and Radosavljević, Vladimir and Radanović, Oliver and Ivetić, Vojin and Pavlović, Ivan and Žutić, Milenko",
year = "2013",
abstract = "Alysiella filiformis is considered a common resident in the oral cavities of many animals. All reports of Alysiella indicate that it is restricted to the oral cavity of warm-blooded vertebrates, where it apparently is nonpathogenic. However, increased losses of young pigs occured in one farm in Serbia. Spumous content in bronchia and partly clotted blood in blood vesels of the lungs were present. Characteristic signs of oedema disease were present and E. coli serogroup 0139 was isolated. Furthermore, Alysiella filiformis was the single agent isolated from the lungs of diseased pigs. This is the first isolation of Alysiella filiformis from pig lungs.",
publisher = "Kafkas Univ, Veteriner Fakultesi Dergisi, Kars",
journal = "Kafkas Universitesi Veteriner Fakultesi Dergisi",
title = "A Case Report: Isolation of Alysiella filiformis from Pig's Lungs",
pages = "723-721",
number = "4",
volume = "19",
doi = "10.9775/kvfd.2013.8648",
url = "conv_418"
}
Žutić, J., Radosavljević, V., Radanović, O., Ivetić, V., Pavlović, I.,& Žutić, M.. (2013). A Case Report: Isolation of Alysiella filiformis from Pig's Lungs. in Kafkas Universitesi Veteriner Fakultesi Dergisi
Kafkas Univ, Veteriner Fakultesi Dergisi, Kars., 19(4), 721-723.
https://doi.org/10.9775/kvfd.2013.8648
conv_418
Žutić J, Radosavljević V, Radanović O, Ivetić V, Pavlović I, Žutić M. A Case Report: Isolation of Alysiella filiformis from Pig's Lungs. in Kafkas Universitesi Veteriner Fakultesi Dergisi. 2013;19(4):721-723.
doi:10.9775/kvfd.2013.8648
conv_418 .
Žutić, Jadranka, Radosavljević, Vladimir, Radanović, Oliver, Ivetić, Vojin, Pavlović, Ivan, Žutić, Milenko, "A Case Report: Isolation of Alysiella filiformis from Pig's Lungs" in Kafkas Universitesi Veteriner Fakultesi Dergisi, 19, no. 4 (2013):721-723,
https://doi.org/10.9775/kvfd.2013.8648 .,
conv_418 .
1

Parazitska fauna svinja u organskom držanju

Pavlović, Ivan; Savić, Božidar; Rogožarski, Dragan; Bojkovski, Jovan; Ivetić, Vojin; Radanović, Oliver; Žutić, Milenko; Stokić-Nikolić, Slavonka; Jezdimirović, Nemanja; Cvetojević, Đorđe

(Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad, 2013)

TY  - JOUR
AU  - Pavlović, Ivan
AU  - Savić, Božidar
AU  - Rogožarski, Dragan
AU  - Bojkovski, Jovan
AU  - Ivetić, Vojin
AU  - Radanović, Oliver
AU  - Žutić, Milenko
AU  - Stokić-Nikolić, Slavonka
AU  - Jezdimirović, Nemanja
AU  - Cvetojević, Đorđe
PY  - 2013
UR  - https://reponivs.nivs.rs/handle/123456789/236
AB  - Parazitske infekcije predstavljaju stalne pratioce svinjarske proizvodnje, nezavisno od načina držanja. Organska proizvodnja i držanje uslovljavaju da svinje imaju direktan dodir sa mnoštvom prelaznih domaćina parazita a samim tim da su kod njih parazitske infekcije češće. U najvećoj meri ovde se javljaju biohelminti od kojih su najvažniji predstavnici roda Metastrongylus, uzročnici verminoznog gastrita (Ascarops strongyllinae, Physocephalus sexalatus and Hyostrongylus rubidus), Oesophagostomum spp. i Macracanthorhynchus hirudinaceus. Ovde se takođe javljaju i Acaris suum, i drugi helminti u manjem obimu.
AB  - Parasites infection were permanent health problem at swine production at exensive and farm breeding. Organic breeding induced permanent contact of swine and intermeditae host of numerous parasites and those infection were more frequent at these breeding condition. In most cases there were presented biohelminths from genus Metastrongylus, nematode which cause gastritis verminosa (Ascarops strongyllinae, Physocephalus sexalatus and Hyostrongylus rubidus), Oesophagostomum spp. And Macracanthorhynchus hirudinaceus. There were presented at lover rate of infection Acaris suum,. and other helminths.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Savremena poljoprivreda
T1  - Parazitska fauna svinja u organskom držanju
T1  - Parasites fauna of swine at organic breeding
EP  - 126
IS  - 1-2
SP  - 118
VL  - 62
UR  - conv_21
ER  - 
@article{
author = "Pavlović, Ivan and Savić, Božidar and Rogožarski, Dragan and Bojkovski, Jovan and Ivetić, Vojin and Radanović, Oliver and Žutić, Milenko and Stokić-Nikolić, Slavonka and Jezdimirović, Nemanja and Cvetojević, Đorđe",
year = "2013",
abstract = "Parazitske infekcije predstavljaju stalne pratioce svinjarske proizvodnje, nezavisno od načina držanja. Organska proizvodnja i držanje uslovljavaju da svinje imaju direktan dodir sa mnoštvom prelaznih domaćina parazita a samim tim da su kod njih parazitske infekcije češće. U najvećoj meri ovde se javljaju biohelminti od kojih su najvažniji predstavnici roda Metastrongylus, uzročnici verminoznog gastrita (Ascarops strongyllinae, Physocephalus sexalatus and Hyostrongylus rubidus), Oesophagostomum spp. i Macracanthorhynchus hirudinaceus. Ovde se takođe javljaju i Acaris suum, i drugi helminti u manjem obimu., Parasites infection were permanent health problem at swine production at exensive and farm breeding. Organic breeding induced permanent contact of swine and intermeditae host of numerous parasites and those infection were more frequent at these breeding condition. In most cases there were presented biohelminths from genus Metastrongylus, nematode which cause gastritis verminosa (Ascarops strongyllinae, Physocephalus sexalatus and Hyostrongylus rubidus), Oesophagostomum spp. And Macracanthorhynchus hirudinaceus. There were presented at lover rate of infection Acaris suum,. and other helminths.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Savremena poljoprivreda",
title = "Parazitska fauna svinja u organskom držanju, Parasites fauna of swine at organic breeding",
pages = "126-118",
number = "1-2",
volume = "62",
url = "conv_21"
}
Pavlović, I., Savić, B., Rogožarski, D., Bojkovski, J., Ivetić, V., Radanović, O., Žutić, M., Stokić-Nikolić, S., Jezdimirović, N.,& Cvetojević, Đ.. (2013). Parazitska fauna svinja u organskom držanju. in Savremena poljoprivreda
Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad., 62(1-2), 118-126.
conv_21
Pavlović I, Savić B, Rogožarski D, Bojkovski J, Ivetić V, Radanović O, Žutić M, Stokić-Nikolić S, Jezdimirović N, Cvetojević Đ. Parazitska fauna svinja u organskom držanju. in Savremena poljoprivreda. 2013;62(1-2):118-126.
conv_21 .
Pavlović, Ivan, Savić, Božidar, Rogožarski, Dragan, Bojkovski, Jovan, Ivetić, Vojin, Radanović, Oliver, Žutić, Milenko, Stokić-Nikolić, Slavonka, Jezdimirović, Nemanja, Cvetojević, Đorđe, "Parazitska fauna svinja u organskom držanju" in Savremena poljoprivreda, 62, no. 1-2 (2013):118-126,
conv_21 .

Mikromorfološke promene na opnama embrioniranih ćurećih jaja inficiranim sa Aspergillus fumigatus i njihov značaj za embrionalno preživljavanje

Jezdimirović, Nemanja; Kureljušić, Branislav; Kureljušić, Jasna; Jakić-Dimić, Dobrila; Ilić, Živka Đ.; Miljković, Biljana; Radanović, Oliver; Cvetojević, Đorđe; Ivetić, Vojin; Jezdimirović, Milanka

(Matica srpska, Novi Sad, 2013)

TY  - JOUR
AU  - Jezdimirović, Nemanja
AU  - Kureljušić, Branislav
AU  - Kureljušić, Jasna
AU  - Jakić-Dimić, Dobrila
AU  - Ilić, Živka Đ.
AU  - Miljković, Biljana
AU  - Radanović, Oliver
AU  - Cvetojević, Đorđe
AU  - Ivetić, Vojin
AU  - Jezdimirović, Milanka
PY  - 2013
UR  - https://reponivs.nivs.rs/handle/123456789/224
AB  - Aspergiloza je često gljivično oboljenje mladih, ali mogu da obole i odrasle jedinke. Infekcija može da nastane već nakon izleganja, udisanjem spora aspergilusa prisutnih u kontaminiranom inkubatoru, ili kasnije, sporama iz fecesa, prostirke, prašine ili hrane. Inače spore mogu da penetriraju i ljusku jajeta i dođu u njegovu unutrašnjost. Bolest se karakteriše zapaljenjem, pre svega respiratornog sistema, mada ponekad protiče i u generalizovanoj formi. Patološke promene u vidu multipnih žućkastih čvorića lokalizovane su najčešće u plućima, vazdušnim kesama, a mogu biti zahvaćeni slezina, mozak, potkožno tkivo i oči. Aspergiloza se teško leči zbog čega je neophodno da se preduzmu sve preventivne mere kojima se smanjuju ili eliminišu uslovi neophodni za nastanak bolesti. Našim istraživanjem je obuhvaćeno 30 embrioniranih ćurećih jaja, iz kojih se u toku inkubacionog perioda nisu izlegli ćurići, a sa ciljem da se utvrdi uzrok embrionalnog mortaliteta. Jaja su poticala od roditeljskog jata ćuraka inkubiranih u inkubatorskoj stanici iz okoline Beograda. Svih 30 jaja je otvoreno, a kod 16 makroskopskim pregledom je ustanovljeno zadebljanje jajčanih opni u području vazdušne komore koja je sa spoljašnje strane difuzno ili cirkumskriptno prekrivena crno-sivim ili sivo-zelenim kolonijama odnosno rastom plesni. Kod tri slučaja veći deo vazdušne komore ispunjavali su razvojni elementi plesni. Za mikroskopsko ispitivanje uzete su promenjene jajčane opne i obrađene standardnom histološkom tehnikom, a dobijeni tkivni rezovi obojeni su HE, PAS i Grocott metodom. Mikroskopskom slikom dominiralo je zadebljanje jajčanih opni kao posledica infiltracije spletom hifa od kojih mnoge dublje penetriraju prema embrionu. Većina njih na izlazu iz opni zauzima vertikalni položaj. Na spoljašnjoj površini opni jasno su se videli slobodni elementi plesni (konidijalne glave sa fijalidama i sporama na njima). Iz sadržaja vazdušnih komora izolovan je Aspergillus fumigatus. Ovako promenjene opne jajeta sigurno su odgovorne za embrionalno uginuće koje se u ovom slučaju, sudeći po veličini embriona, dogodilo od sedmog do desetog dana starosti.
AB  - Aspergillosis is a frequent fungal disease of young and adult poultry in our commercial flocks. Infection can occur after hatching by inhalation of Aspergillus conidia which can be present in contaminated hatcheries, or later, by spores from moldy litter, dust, faeces or feed. Spores from the surface of egg shell can penetrate inside of an egg. The main characteristic of aspergillosis is granulomatous inflammation of respiratory system, although generalized form is possible as well. Multiple yellow nodules can be seen as major patomorphological changes and they are usually localized in lungs, air sacs, and can also be found in spleen, brain, subcutis and eyes. Egg embryos are quite susceptible to infection by Aspergillus fumigatus during incubation. In this study, the history of a case on one local farm with mortality rate of 7.2% in the turkey flock is presented. At the same time, 28 day old 30 incubated hatching turkey eggs were sampled, that were unable to hatch. The aim of the present work was to determine the cause and to identify the agent of embryonic mortality. Total of the 30 eggs were opened, and 16 of them had thickened egg membranes in the area of air sac. Membrane thickening was visible and circumscript or diffuse presence of black-grey or grey-green fungal growth was observed. Only 3 samples air sacs were filled with developed stages of fungi. To evaluate histopathological lesions, changed egg membranes were processed by standard histological technique. Dominant microscopic finding was thickening of egg membranes as a consequence of fungal growth and many of them penetrated deep towards embryo. Most of the hyphae were growing vertically through membranes. On the outside surface of the membranes, the elements of fungi (conidial heads with phialids and spores on them), could be clearly observed. These changes were responsible for embryonic death, which on the basis of the size of dead embryos occurred between 7th and 10th day of incubation. Aspergillus fumigatus was isolated and identified from the content of air sacs.
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Mikromorfološke promene na opnama embrioniranih ćurećih jaja inficiranim sa Aspergillus fumigatus i njihov značaj za embrionalno preživljavanje
T1  - Micromorphological changes on the embryonic membranes of turkey eggs infected with Aspergillus fumigatus and their importance for embryonic survival
EP  - 271
IS  - 124
SP  - 263
UR  - conv_127
ER  - 
@article{
author = "Jezdimirović, Nemanja and Kureljušić, Branislav and Kureljušić, Jasna and Jakić-Dimić, Dobrila and Ilić, Živka Đ. and Miljković, Biljana and Radanović, Oliver and Cvetojević, Đorđe and Ivetić, Vojin and Jezdimirović, Milanka",
year = "2013",
abstract = "Aspergiloza je često gljivično oboljenje mladih, ali mogu da obole i odrasle jedinke. Infekcija može da nastane već nakon izleganja, udisanjem spora aspergilusa prisutnih u kontaminiranom inkubatoru, ili kasnije, sporama iz fecesa, prostirke, prašine ili hrane. Inače spore mogu da penetriraju i ljusku jajeta i dođu u njegovu unutrašnjost. Bolest se karakteriše zapaljenjem, pre svega respiratornog sistema, mada ponekad protiče i u generalizovanoj formi. Patološke promene u vidu multipnih žućkastih čvorića lokalizovane su najčešće u plućima, vazdušnim kesama, a mogu biti zahvaćeni slezina, mozak, potkožno tkivo i oči. Aspergiloza se teško leči zbog čega je neophodno da se preduzmu sve preventivne mere kojima se smanjuju ili eliminišu uslovi neophodni za nastanak bolesti. Našim istraživanjem je obuhvaćeno 30 embrioniranih ćurećih jaja, iz kojih se u toku inkubacionog perioda nisu izlegli ćurići, a sa ciljem da se utvrdi uzrok embrionalnog mortaliteta. Jaja su poticala od roditeljskog jata ćuraka inkubiranih u inkubatorskoj stanici iz okoline Beograda. Svih 30 jaja je otvoreno, a kod 16 makroskopskim pregledom je ustanovljeno zadebljanje jajčanih opni u području vazdušne komore koja je sa spoljašnje strane difuzno ili cirkumskriptno prekrivena crno-sivim ili sivo-zelenim kolonijama odnosno rastom plesni. Kod tri slučaja veći deo vazdušne komore ispunjavali su razvojni elementi plesni. Za mikroskopsko ispitivanje uzete su promenjene jajčane opne i obrađene standardnom histološkom tehnikom, a dobijeni tkivni rezovi obojeni su HE, PAS i Grocott metodom. Mikroskopskom slikom dominiralo je zadebljanje jajčanih opni kao posledica infiltracije spletom hifa od kojih mnoge dublje penetriraju prema embrionu. Većina njih na izlazu iz opni zauzima vertikalni položaj. Na spoljašnjoj površini opni jasno su se videli slobodni elementi plesni (konidijalne glave sa fijalidama i sporama na njima). Iz sadržaja vazdušnih komora izolovan je Aspergillus fumigatus. Ovako promenjene opne jajeta sigurno su odgovorne za embrionalno uginuće koje se u ovom slučaju, sudeći po veličini embriona, dogodilo od sedmog do desetog dana starosti., Aspergillosis is a frequent fungal disease of young and adult poultry in our commercial flocks. Infection can occur after hatching by inhalation of Aspergillus conidia which can be present in contaminated hatcheries, or later, by spores from moldy litter, dust, faeces or feed. Spores from the surface of egg shell can penetrate inside of an egg. The main characteristic of aspergillosis is granulomatous inflammation of respiratory system, although generalized form is possible as well. Multiple yellow nodules can be seen as major patomorphological changes and they are usually localized in lungs, air sacs, and can also be found in spleen, brain, subcutis and eyes. Egg embryos are quite susceptible to infection by Aspergillus fumigatus during incubation. In this study, the history of a case on one local farm with mortality rate of 7.2% in the turkey flock is presented. At the same time, 28 day old 30 incubated hatching turkey eggs were sampled, that were unable to hatch. The aim of the present work was to determine the cause and to identify the agent of embryonic mortality. Total of the 30 eggs were opened, and 16 of them had thickened egg membranes in the area of air sac. Membrane thickening was visible and circumscript or diffuse presence of black-grey or grey-green fungal growth was observed. Only 3 samples air sacs were filled with developed stages of fungi. To evaluate histopathological lesions, changed egg membranes were processed by standard histological technique. Dominant microscopic finding was thickening of egg membranes as a consequence of fungal growth and many of them penetrated deep towards embryo. Most of the hyphae were growing vertically through membranes. On the outside surface of the membranes, the elements of fungi (conidial heads with phialids and spores on them), could be clearly observed. These changes were responsible for embryonic death, which on the basis of the size of dead embryos occurred between 7th and 10th day of incubation. Aspergillus fumigatus was isolated and identified from the content of air sacs.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Mikromorfološke promene na opnama embrioniranih ćurećih jaja inficiranim sa Aspergillus fumigatus i njihov značaj za embrionalno preživljavanje, Micromorphological changes on the embryonic membranes of turkey eggs infected with Aspergillus fumigatus and their importance for embryonic survival",
pages = "271-263",
number = "124",
url = "conv_127"
}
Jezdimirović, N., Kureljušić, B., Kureljušić, J., Jakić-Dimić, D., Ilić, Ž. Đ., Miljković, B., Radanović, O., Cvetojević, Đ., Ivetić, V.,& Jezdimirović, M.. (2013). Mikromorfološke promene na opnama embrioniranih ćurećih jaja inficiranim sa Aspergillus fumigatus i njihov značaj za embrionalno preživljavanje. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(124), 263-271.
conv_127
Jezdimirović N, Kureljušić B, Kureljušić J, Jakić-Dimić D, Ilić ŽĐ, Miljković B, Radanović O, Cvetojević Đ, Ivetić V, Jezdimirović M. Mikromorfološke promene na opnama embrioniranih ćurećih jaja inficiranim sa Aspergillus fumigatus i njihov značaj za embrionalno preživljavanje. in Zbornik Matice srpske za prirodne nauke. 2013;(124):263-271.
conv_127 .
Jezdimirović, Nemanja, Kureljušić, Branislav, Kureljušić, Jasna, Jakić-Dimić, Dobrila, Ilić, Živka Đ., Miljković, Biljana, Radanović, Oliver, Cvetojević, Đorđe, Ivetić, Vojin, Jezdimirović, Milanka, "Mikromorfološke promene na opnama embrioniranih ćurećih jaja inficiranim sa Aspergillus fumigatus i njihov značaj za embrionalno preživljavanje" in Zbornik Matice srpske za prirodne nauke, no. 124 (2013):263-271,
conv_127 .

Echinococcus multilocularis in the European beaver (Castor fibre L.) from Serbia: first report

Ćirović, Duško; Pavlović, Ivan; Kulišić, Zoran; Ivetić, Vojin; Penezić, Aleksandra; Cosić, N.

(BMJ Publishing Group, 2013)

TY  - JOUR
AU  - Ćirović, Duško
AU  - Pavlović, Ivan
AU  - Kulišić, Zoran
AU  - Ivetić, Vojin
AU  - Penezić, Aleksandra
AU  - Cosić, N.
PY  - 2013
UR  - https://reponivs.nivs.rs/handle/123456789/250
PB  - BMJ Publishing Group
T2  - Veterinary Record Case Reports
T1  - Echinococcus multilocularis in the European beaver (Castor fibre L.) from Serbia: first report
IS  - 1
VL  - 1
DO  - 10.1136/vetreccr.100879rep
UR  - conv_680
ER  - 
@article{
author = "Ćirović, Duško and Pavlović, Ivan and Kulišić, Zoran and Ivetić, Vojin and Penezić, Aleksandra and Cosić, N.",
year = "2013",
publisher = "BMJ Publishing Group",
journal = "Veterinary Record Case Reports",
title = "Echinococcus multilocularis in the European beaver (Castor fibre L.) from Serbia: first report",
number = "1",
volume = "1",
doi = "10.1136/vetreccr.100879rep",
url = "conv_680"
}
Ćirović, D., Pavlović, I., Kulišić, Z., Ivetić, V., Penezić, A.,& Cosić, N.. (2013). Echinococcus multilocularis in the European beaver (Castor fibre L.) from Serbia: first report. in Veterinary Record Case Reports
BMJ Publishing Group., 1(1).
https://doi.org/10.1136/vetreccr.100879rep
conv_680
Ćirović D, Pavlović I, Kulišić Z, Ivetić V, Penezić A, Cosić N. Echinococcus multilocularis in the European beaver (Castor fibre L.) from Serbia: first report. in Veterinary Record Case Reports. 2013;1(1).
doi:10.1136/vetreccr.100879rep
conv_680 .
Ćirović, Duško, Pavlović, Ivan, Kulišić, Zoran, Ivetić, Vojin, Penezić, Aleksandra, Cosić, N., "Echinococcus multilocularis in the European beaver (Castor fibre L.) from Serbia: first report" in Veterinary Record Case Reports, 1, no. 1 (2013),
https://doi.org/10.1136/vetreccr.100879rep .,
conv_680 .
2
15
2

Virusni hepatitis E - oboljenje ljudi i životinja

Kureljušić, Branislav; Ivetić, Vojin; Savić, Božidar; Kureljušić, Jasna; Jezdimirović, Nemanja

(Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd, 2012)

TY  - JOUR
AU  - Kureljušić, Branislav
AU  - Ivetić, Vojin
AU  - Savić, Božidar
AU  - Kureljušić, Jasna
AU  - Jezdimirović, Nemanja
PY  - 2012
UR  - https://reponivs.nivs.rs/handle/123456789/219
AB  - Virus hepatitisa E je ubikvitaran u svim područjima sveta u kojima postoji proizvodnja svinja. Infekcija se javlja kod nekoliko životinjskih vrsta i uglavnom protiče asimptomatski. Sojevi virusa izolovani od svinja i ljudi su genetski slični, što ukazuje na potencijalni zoonotski karakter bolesti i mogućnost da su svinje, a možda i druge vrste životinja obolele od virusnog hepatitisa E izvor infekcije za ljude. Virus svinjskog hepatitisa E, koji je sličan virusu hepatitisa E kod ljudi, prvi put je izolovan i opisan u SAD 1997. godine. Infekcija svinja izazvana virusom hepatitisa E nastaje fekooralnim putem, ingestijom hrane i vode kontaminirane virusom ili direktnim kontaktom između inficiranih i zdravih životinja. Patogeneza hepatitisa E kod svinja se razlikuje od patogeneze ove infekcije kod ljudi i nije dovoljno istražena. Mada je virusni hepatitis E kod svinja definisan kao supkliničko oboljenje, neki autori opisuju promene u koncentracijama pojedinih biohemijskih parametara u krvnom serumu inficiranih svinja. Histološki se u jetri inficiranih svinja uočavaju blaga do umerena limfocitno-plazmaćelijska infiltracija i fokalna područja nekroze hepatocita. Virusni hepatitis E je endemsko oboljenje ljudi u Aziji, Africi i Latinskoj Americi. U razvijenim zemljama hepatitis E se sporadično javlja kod ljudi, ali vremenom dobija na značaju naročito u Japanu, Severnoj Americi i Evropi, zbog putovanja stanovništva iz tih područja u endemske regione ili zbog konzumiranja termički netretiranog mesa divljih svinja kao i proizvoda od termički netretiranog mesa. Bezbednost hrane jeste veoma značajan aspekt u epidemiologiji virusnog hepatitisa E, s obzirom na to da meso i proizvodi od svinjskog mesa mogu biti kontaminirani virusom hepatitisa E. Dodatni pokazatelji zoonotskog potencijala virusa, a zbog čega se ovo oboljenje svrstava u grupu profesionalnih oboljenja farmera i doktora veterinarske medicine, jeste nalaz antitela na virus hepatitisa E kod farmera i doktora veterinarske medicine koji rade na farmama svinja, a pri tome ne pokazuju kliničke znake oboljenja. S obzirom na činjenicu da je virusni hepatitis E potvrđen na farmama svinja u Srbiji i zemljama u okruženju sa epizootiološkog i epidemiološkog stanovišta trebalo bi striktno poštovati biosigurnosne mere i pridržavati se principa dobre proizvođačke i higijenske prakse na farmama svinja. U budućnosti bi trebalo u zakonsku regulativu naše zemlje uvrstiti i ovo oboljenje u svrhu dobijanja higijenski bezbednih namirnica životinjskog porekla.
AB  - The hepatitis E virus is ubiquitous in all parts of the world where pig production exists. The infection occurs in several animal species and its course is mostly asymptomatic. Viral strains isolated from pigs and humans are genetically similar, which indicates a potential zoonotic nature of the disease, and the possibility that pigs, and perhaps also other species of animals diseased with viral hepatitis E are a source of infection to humans. The pig hepatitis E virus, which is similar to the hepatitis E virus in humans, was isolated and described for the first time in the USA in 1997. The infection of pigs with hepatitis E virus occurs through faeco-oral transmission, by ingestion of feed and water contaminated with the virus, or through direct contact between infected and healthy animals. The pathogenesis of this infection in pigs differs from its pathogenesis in humans and it has not been sufficiently examined in all its aspects. Even though viral hepatitis E in pigs has been described as a subclinical disease, some authors describe changes in the concentration of certain biochemical parameters in blood serum of the infected pigs. Histologically, a mild to moderate lymphotic-plasma cellular infiltration is observed in livers of infected pigs, as well as focal areas of hepatocyte necrosis. Viral hepatitis E is an endemic disease of humans in Asia, Africa, and Latin America. In developed countries, hepatitis E sporadically occurs in humans, but it is becoming of increasing importance in particular in Japan, North America, and Europe, because the populations of these areas travel extensively to the endemic regions or as a result of the consumption of thermally untreated meat of wild boar and products made from thermally untreated meat. Pork products can be contaminated with hepatitis E virus. Further proof that indicates the zoonotic potential of this virus and places this diseases among the group of professional diseases of farmers and veterinarians is the finding of antibodies to hepatitis E virus in farmers and veterinarians who work on pig farms without showing any clinical signs of the disease. Having in mind the fact that viral hepatitis E has been proven in pig farms in Serbia and neighboruign countries, there should be strict respect of biosecutiry measures from the episootiological and epidemiological aspects, and the principle of good production and hygiene practice should be adhered to on pig farms. This disease should in future also be included in the legal regulations of our country in order to ensure the production of products of animal origin that are safe from the aspect of hygiene.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski glasnik
T1  - Virusni hepatitis E - oboljenje ljudi i životinja
T1  - Viral hepatitis E: A disease of humans and animals
EP  - 462
IS  - 5-6
SP  - 449
VL  - 66
DO  - 10.2298/VETGL1206449K
UR  - conv_95
ER  - 
@article{
author = "Kureljušić, Branislav and Ivetić, Vojin and Savić, Božidar and Kureljušić, Jasna and Jezdimirović, Nemanja",
year = "2012",
abstract = "Virus hepatitisa E je ubikvitaran u svim područjima sveta u kojima postoji proizvodnja svinja. Infekcija se javlja kod nekoliko životinjskih vrsta i uglavnom protiče asimptomatski. Sojevi virusa izolovani od svinja i ljudi su genetski slični, što ukazuje na potencijalni zoonotski karakter bolesti i mogućnost da su svinje, a možda i druge vrste životinja obolele od virusnog hepatitisa E izvor infekcije za ljude. Virus svinjskog hepatitisa E, koji je sličan virusu hepatitisa E kod ljudi, prvi put je izolovan i opisan u SAD 1997. godine. Infekcija svinja izazvana virusom hepatitisa E nastaje fekooralnim putem, ingestijom hrane i vode kontaminirane virusom ili direktnim kontaktom između inficiranih i zdravih životinja. Patogeneza hepatitisa E kod svinja se razlikuje od patogeneze ove infekcije kod ljudi i nije dovoljno istražena. Mada je virusni hepatitis E kod svinja definisan kao supkliničko oboljenje, neki autori opisuju promene u koncentracijama pojedinih biohemijskih parametara u krvnom serumu inficiranih svinja. Histološki se u jetri inficiranih svinja uočavaju blaga do umerena limfocitno-plazmaćelijska infiltracija i fokalna područja nekroze hepatocita. Virusni hepatitis E je endemsko oboljenje ljudi u Aziji, Africi i Latinskoj Americi. U razvijenim zemljama hepatitis E se sporadično javlja kod ljudi, ali vremenom dobija na značaju naročito u Japanu, Severnoj Americi i Evropi, zbog putovanja stanovništva iz tih područja u endemske regione ili zbog konzumiranja termički netretiranog mesa divljih svinja kao i proizvoda od termički netretiranog mesa. Bezbednost hrane jeste veoma značajan aspekt u epidemiologiji virusnog hepatitisa E, s obzirom na to da meso i proizvodi od svinjskog mesa mogu biti kontaminirani virusom hepatitisa E. Dodatni pokazatelji zoonotskog potencijala virusa, a zbog čega se ovo oboljenje svrstava u grupu profesionalnih oboljenja farmera i doktora veterinarske medicine, jeste nalaz antitela na virus hepatitisa E kod farmera i doktora veterinarske medicine koji rade na farmama svinja, a pri tome ne pokazuju kliničke znake oboljenja. S obzirom na činjenicu da je virusni hepatitis E potvrđen na farmama svinja u Srbiji i zemljama u okruženju sa epizootiološkog i epidemiološkog stanovišta trebalo bi striktno poštovati biosigurnosne mere i pridržavati se principa dobre proizvođačke i higijenske prakse na farmama svinja. U budućnosti bi trebalo u zakonsku regulativu naše zemlje uvrstiti i ovo oboljenje u svrhu dobijanja higijenski bezbednih namirnica životinjskog porekla., The hepatitis E virus is ubiquitous in all parts of the world where pig production exists. The infection occurs in several animal species and its course is mostly asymptomatic. Viral strains isolated from pigs and humans are genetically similar, which indicates a potential zoonotic nature of the disease, and the possibility that pigs, and perhaps also other species of animals diseased with viral hepatitis E are a source of infection to humans. The pig hepatitis E virus, which is similar to the hepatitis E virus in humans, was isolated and described for the first time in the USA in 1997. The infection of pigs with hepatitis E virus occurs through faeco-oral transmission, by ingestion of feed and water contaminated with the virus, or through direct contact between infected and healthy animals. The pathogenesis of this infection in pigs differs from its pathogenesis in humans and it has not been sufficiently examined in all its aspects. Even though viral hepatitis E in pigs has been described as a subclinical disease, some authors describe changes in the concentration of certain biochemical parameters in blood serum of the infected pigs. Histologically, a mild to moderate lymphotic-plasma cellular infiltration is observed in livers of infected pigs, as well as focal areas of hepatocyte necrosis. Viral hepatitis E is an endemic disease of humans in Asia, Africa, and Latin America. In developed countries, hepatitis E sporadically occurs in humans, but it is becoming of increasing importance in particular in Japan, North America, and Europe, because the populations of these areas travel extensively to the endemic regions or as a result of the consumption of thermally untreated meat of wild boar and products made from thermally untreated meat. Pork products can be contaminated with hepatitis E virus. Further proof that indicates the zoonotic potential of this virus and places this diseases among the group of professional diseases of farmers and veterinarians is the finding of antibodies to hepatitis E virus in farmers and veterinarians who work on pig farms without showing any clinical signs of the disease. Having in mind the fact that viral hepatitis E has been proven in pig farms in Serbia and neighboruign countries, there should be strict respect of biosecutiry measures from the episootiological and epidemiological aspects, and the principle of good production and hygiene practice should be adhered to on pig farms. This disease should in future also be included in the legal regulations of our country in order to ensure the production of products of animal origin that are safe from the aspect of hygiene.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski glasnik",
title = "Virusni hepatitis E - oboljenje ljudi i životinja, Viral hepatitis E: A disease of humans and animals",
pages = "462-449",
number = "5-6",
volume = "66",
doi = "10.2298/VETGL1206449K",
url = "conv_95"
}
Kureljušić, B., Ivetić, V., Savić, B., Kureljušić, J.,& Jezdimirović, N.. (2012). Virusni hepatitis E - oboljenje ljudi i životinja. in Veterinarski glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 66(5-6), 449-462.
https://doi.org/10.2298/VETGL1206449K
conv_95
Kureljušić B, Ivetić V, Savić B, Kureljušić J, Jezdimirović N. Virusni hepatitis E - oboljenje ljudi i životinja. in Veterinarski glasnik. 2012;66(5-6):449-462.
doi:10.2298/VETGL1206449K
conv_95 .
Kureljušić, Branislav, Ivetić, Vojin, Savić, Božidar, Kureljušić, Jasna, Jezdimirović, Nemanja, "Virusni hepatitis E - oboljenje ljudi i životinja" in Veterinarski glasnik, 66, no. 5-6 (2012):449-462,
https://doi.org/10.2298/VETGL1206449K .,
conv_95 .

Procena energetskog statusa krava na osnovu telesne kondicije i parametara metaboličkog profila

Prodanović, Radiša; Sladojević, Ž.; Kirovski, Danijela; Vujanac, Ivan; Ivetić, Vojin; Savić, Božidar; Kureljušić, Branislav; Stevančević, M.

(Institut za stočarstvo, Beograd, 2012)

TY  - JOUR
AU  - Prodanović, Radiša
AU  - Sladojević, Ž.
AU  - Kirovski, Danijela
AU  - Vujanac, Ivan
AU  - Ivetić, Vojin
AU  - Savić, Božidar
AU  - Kureljušić, Branislav
AU  - Stevančević, M.
PY  - 2012
UR  - https://reponivs.nivs.rs/handle/123456789/213
AB  - Cilj istraživanja bio je da se ukaže na značaj metaboličkog profila i telesne kondicije u oceni energetskog statusa visoko-mlečnih krava holštajn rase. Za ispitivanje su odabrane tri grupe po sedam krava. Uzorci krvi uzimani su u zasušenju (15 dana pre očekivanog teljenja), ranom puerperijumu (7 dana nakon teljenja) i 60. dana laktacije. U uzorcima sveže krvi određivana je koncentracija glukoze i beta hidroksi-buterne kiseline (BHBA), a u uzorcima krvnog seruma koncentracija ukupnih proteina, ureje i ukupnog bilirubina. Rezultati ocene telesne kondicije pokazuju da su krave u periodu zasušenja preuhranjene (X=4,03±0,29 poena), dok su u puerperijumu (X=2,85±0,46 poena) i 60. dana laktacije (X=3,12±0,33 poena) u značajno slabijoj telesnoj kondiciji (p LT 0,001 u odnosu na zasušenje). Rezultati ispitivanja parametara metaboličkog profila ukazuju da je koncentracija proteina jedino u periodu ranog puerperijuma bila niža od fiziološke vrednosti (X=56,87±3,29 g/l). Koncentracija glukoze nije se statistički značajno menjala tokom ispitivanih perioda, dok je neposredno posle teljenja došlo do značajnog povećanja koncentracije BHBA (p LT 0,001 u odnosu na zasušenje). Uremija u periodu zasušenja (X=7,73±2,34 mmol/l), ranom puerperijumu (X=8,07±3,09 mmol/l) i 60. dana laktacije (X=7,41±2,03 mmol/l) bila je veća od gornje fiziološke granice. Koncentracija ukupnog bilirubina u periodu zasušenja je bila značajno niža (p LT 0,001) u odnosu na oba ispitivana perioda nakon teljenja. Na osnovu dobijenih rezultata može se zaključiti da krave nisu adekvatno pripremljene za nastupajuću laktaciju, zbog čega je kod njih došlo do značajnog odstupanja u telesnoj kondiciji i vrednostima parametara metaboličkog profila. Ova odstupanja ukazuju na nedovoljnu sposobnost životinja da se prilagode u uslovima negativnog bilansa energije na početku laktacije.
AB  - The aim of this study was to assess the significance of body condition scoring and metabolic profile test for estimation of energy status of healthy high-yielding dairy cows. Twenty one healthy cows (primiparous and secundiparous) were divided into three groups: dry cows, early puerperal cows and early lactating cows. Cow's energy status was estimated by the analysis of blood samples for beta-hydroxybutirate (BHBA) and glucose. Additionally, urea, total bilirubine and total protein were measured in blood serum samples. According to body condition scores (BCS) results dry cows were overweight (4.03±0.29 points). Immediately after calving, cows lost their weight significantly, since BCS was 2.85±0.46 points at puerperal period and 3.12±0.33 points at day 60 of lactation (p LT 0.001 compared to dry period, respectively). Glucose concentration did not change significantly between dry and puerperal period, while BHBA increased significantly (0.46±0.14 mmol/l at dry period to 1.08±0.21 mmol/l at puerperal period; p LT 0.001). Urea concentration did not change significantly during examined period. Total bilirubin concentration significantly increased from dry (6.31±0.37 mmol/l) to puerperal period (7.63±2.52 mmol/l; p LT 0.001 compared to dry period) and remained high until day 60 of lactation (7.62±0.13 mmol/l; p LT 0.001 compared to dry period). Total protein concentration decreased from dry to puerperal period (69.59±6.14 g/l to 58.87±3.29 g/l; p LT 0.001). According to obtained results it can be concluded that cows were not in adequate energy status during transition period. Our results also indicate that, body condition scoring, BHBA and total bilirubin concentrations can be used as reliable indicators of cow's energy status even when there are not clinically visible health disorders.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Procena energetskog statusa krava na osnovu telesne kondicije i parametara metaboličkog profila
T1  - Use of metabolic profiles and body condition scoring for the assessment of energy status of dairy cows
EP  - 32
IS  - 1
SP  - 25
VL  - 28
DO  - 10.2298/BAH1201025P
UR  - conv_298
ER  - 
@article{
author = "Prodanović, Radiša and Sladojević, Ž. and Kirovski, Danijela and Vujanac, Ivan and Ivetić, Vojin and Savić, Božidar and Kureljušić, Branislav and Stevančević, M.",
year = "2012",
abstract = "Cilj istraživanja bio je da se ukaže na značaj metaboličkog profila i telesne kondicije u oceni energetskog statusa visoko-mlečnih krava holštajn rase. Za ispitivanje su odabrane tri grupe po sedam krava. Uzorci krvi uzimani su u zasušenju (15 dana pre očekivanog teljenja), ranom puerperijumu (7 dana nakon teljenja) i 60. dana laktacije. U uzorcima sveže krvi određivana je koncentracija glukoze i beta hidroksi-buterne kiseline (BHBA), a u uzorcima krvnog seruma koncentracija ukupnih proteina, ureje i ukupnog bilirubina. Rezultati ocene telesne kondicije pokazuju da su krave u periodu zasušenja preuhranjene (X=4,03±0,29 poena), dok su u puerperijumu (X=2,85±0,46 poena) i 60. dana laktacije (X=3,12±0,33 poena) u značajno slabijoj telesnoj kondiciji (p LT 0,001 u odnosu na zasušenje). Rezultati ispitivanja parametara metaboličkog profila ukazuju da je koncentracija proteina jedino u periodu ranog puerperijuma bila niža od fiziološke vrednosti (X=56,87±3,29 g/l). Koncentracija glukoze nije se statistički značajno menjala tokom ispitivanih perioda, dok je neposredno posle teljenja došlo do značajnog povećanja koncentracije BHBA (p LT 0,001 u odnosu na zasušenje). Uremija u periodu zasušenja (X=7,73±2,34 mmol/l), ranom puerperijumu (X=8,07±3,09 mmol/l) i 60. dana laktacije (X=7,41±2,03 mmol/l) bila je veća od gornje fiziološke granice. Koncentracija ukupnog bilirubina u periodu zasušenja je bila značajno niža (p LT 0,001) u odnosu na oba ispitivana perioda nakon teljenja. Na osnovu dobijenih rezultata može se zaključiti da krave nisu adekvatno pripremljene za nastupajuću laktaciju, zbog čega je kod njih došlo do značajnog odstupanja u telesnoj kondiciji i vrednostima parametara metaboličkog profila. Ova odstupanja ukazuju na nedovoljnu sposobnost životinja da se prilagode u uslovima negativnog bilansa energije na početku laktacije., The aim of this study was to assess the significance of body condition scoring and metabolic profile test for estimation of energy status of healthy high-yielding dairy cows. Twenty one healthy cows (primiparous and secundiparous) were divided into three groups: dry cows, early puerperal cows and early lactating cows. Cow's energy status was estimated by the analysis of blood samples for beta-hydroxybutirate (BHBA) and glucose. Additionally, urea, total bilirubine and total protein were measured in blood serum samples. According to body condition scores (BCS) results dry cows were overweight (4.03±0.29 points). Immediately after calving, cows lost their weight significantly, since BCS was 2.85±0.46 points at puerperal period and 3.12±0.33 points at day 60 of lactation (p LT 0.001 compared to dry period, respectively). Glucose concentration did not change significantly between dry and puerperal period, while BHBA increased significantly (0.46±0.14 mmol/l at dry period to 1.08±0.21 mmol/l at puerperal period; p LT 0.001). Urea concentration did not change significantly during examined period. Total bilirubin concentration significantly increased from dry (6.31±0.37 mmol/l) to puerperal period (7.63±2.52 mmol/l; p LT 0.001 compared to dry period) and remained high until day 60 of lactation (7.62±0.13 mmol/l; p LT 0.001 compared to dry period). Total protein concentration decreased from dry to puerperal period (69.59±6.14 g/l to 58.87±3.29 g/l; p LT 0.001). According to obtained results it can be concluded that cows were not in adequate energy status during transition period. Our results also indicate that, body condition scoring, BHBA and total bilirubin concentrations can be used as reliable indicators of cow's energy status even when there are not clinically visible health disorders.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Procena energetskog statusa krava na osnovu telesne kondicije i parametara metaboličkog profila, Use of metabolic profiles and body condition scoring for the assessment of energy status of dairy cows",
pages = "32-25",
number = "1",
volume = "28",
doi = "10.2298/BAH1201025P",
url = "conv_298"
}
Prodanović, R., Sladojević, Ž., Kirovski, D., Vujanac, I., Ivetić, V., Savić, B., Kureljušić, B.,& Stevančević, M.. (2012). Procena energetskog statusa krava na osnovu telesne kondicije i parametara metaboličkog profila. in Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 28(1), 25-32.
https://doi.org/10.2298/BAH1201025P
conv_298
Prodanović R, Sladojević Ž, Kirovski D, Vujanac I, Ivetić V, Savić B, Kureljušić B, Stevančević M. Procena energetskog statusa krava na osnovu telesne kondicije i parametara metaboličkog profila. in Biotechnology in Animal Husbandry. 2012;28(1):25-32.
doi:10.2298/BAH1201025P
conv_298 .
Prodanović, Radiša, Sladojević, Ž., Kirovski, Danijela, Vujanac, Ivan, Ivetić, Vojin, Savić, Božidar, Kureljušić, Branislav, Stevančević, M., "Procena energetskog statusa krava na osnovu telesne kondicije i parametara metaboličkog profila" in Biotechnology in Animal Husbandry, 28, no. 1 (2012):25-32,
https://doi.org/10.2298/BAH1201025P .,
conv_298 .
4

Patomorfološka dijagnostika hemoragične proliferativne enteropatije svinja

Kureljušić, Branislav; Savić, Božidar; Jezdimirović, Nemanja; Radanović, Oliver; Kureljušić, Jasna; Žutić, Milenko; Ivetić, Vojin

(Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad, 2012)

TY  - JOUR
AU  - Kureljušić, Branislav
AU  - Savić, Božidar
AU  - Jezdimirović, Nemanja
AU  - Radanović, Oliver
AU  - Kureljušić, Jasna
AU  - Žutić, Milenko
AU  - Ivetić, Vojin
PY  - 2012
UR  - https://reponivs.nivs.rs/handle/123456789/197
AB  - U ovom radu je makroskopski, histohemijski i imunohistohemijski ispitano 7 uzoraka creva prirodno inficiranih svinja. Patomorfološke promene u vidu zadebljanja crevnog zida i proširenog lumena su ustanovljene na kaudalnom delu jejunuma i ileumu. U lumenu ileuma i kolona su uočeni ugrušci krvi bez prisustva hrane. U rektumu je ustanovljen katranasti feces koji potiče od svarene krvi i crevnog sadržaja. Površina sluznice zahvaćenog dela creva je pokazivala značajno hiperplastično zadebljanje. Erozije sluznice nisu ustanovljene. Histološkim ispitivanjem u proliferisanom epitelu uočena je degeneracija epitelnih ćelija, kao i kongestija i obimna krvavljenja. Pored toga, zapaženo je i nakupljanje hemoragičnog ćelijskog debrisa iznad površine sluznice. Warthin-Starry bojenjem ustanovljene su grupe argirofilnih, blago savijenih štapićastih mikroorganizama u apikalnoj citoplazmi enterocita. Imunohistohemijskim ispitivanjem upotrebom monoklonskog antitela protiv L. intracellularis potvrđeno je prisustvo mikroorganizama u apikalnoj citoplazmi hiperplastičnih enterocita i u lamini propriji. Dijagnoza hemoragične proliferativne enteropatije je postavljena na osnovu karakterističnih histoloških lezija i korišćenjem Warthin-Starry bojenja, kao i upotrebom imunohistohemijske metode, korišćenjem monoklonskog antitela protiv L. intracellularis. Imunohistohemijska metoda i Warthin-Starry metod bojenja mogu biti komplementarne metode za potvrdu infekcije izazvane sa L. intracellularis kod svinja. .
AB  - In this paper seven samples of the intestines originated from the naturally infected pigs, macroscopic, histochemically and immunohistochemically were examined. The caudal part of jejunum and ileum were affected. The affected intestine was thickened and dilated. The lumen of the ileum and colon contained one or more blood clots without feed contents. The rectum contained black tarry feces of mixed blood and digesta. The mucosal surface of the affected portion of intestine was markedly thickened without macroscopic erosions. Histological examination revealed: extensive degeneration, congestion, and hemorrhage within the proliferative epithelium, as well as a marked accumulation of the bloody cellular debris above affected mucosa. Clusters of argyrophilic, slightly curved rod-shaped microorganisms in the apical cytoplasm of enterocytes by Warthin-Starry silver stain were demonstrated. Immunohistochemical staining confirmed the presence of L. intracellularis in the apical cytoplasm of hyperplastic enterocytes and in lamina propria. In conclusion, diagnosis of hemorrhagic proliferative enteropathy is based on detection of the histologic lesions and detection of L. intracellularis by Warthin Starry silver stain, as well as by immunohistochemistry, where immunohistochemistry and Warthin-Starry silver method can be a complementary methods to confirm the diagnosis of L. intracellularis infection in pigs. .
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Savremena poljoprivreda
T1  - Patomorfološka dijagnostika hemoragične proliferativne enteropatije svinja
T1  - Patomorphological diagnosis of hemorrhagic proliferative enteropathy in swine
EP  - 74
IS  - 1-2
SP  - 68
VL  - 61
UR  - conv_20
ER  - 
@article{
author = "Kureljušić, Branislav and Savić, Božidar and Jezdimirović, Nemanja and Radanović, Oliver and Kureljušić, Jasna and Žutić, Milenko and Ivetić, Vojin",
year = "2012",
abstract = "U ovom radu je makroskopski, histohemijski i imunohistohemijski ispitano 7 uzoraka creva prirodno inficiranih svinja. Patomorfološke promene u vidu zadebljanja crevnog zida i proširenog lumena su ustanovljene na kaudalnom delu jejunuma i ileumu. U lumenu ileuma i kolona su uočeni ugrušci krvi bez prisustva hrane. U rektumu je ustanovljen katranasti feces koji potiče od svarene krvi i crevnog sadržaja. Površina sluznice zahvaćenog dela creva je pokazivala značajno hiperplastično zadebljanje. Erozije sluznice nisu ustanovljene. Histološkim ispitivanjem u proliferisanom epitelu uočena je degeneracija epitelnih ćelija, kao i kongestija i obimna krvavljenja. Pored toga, zapaženo je i nakupljanje hemoragičnog ćelijskog debrisa iznad površine sluznice. Warthin-Starry bojenjem ustanovljene su grupe argirofilnih, blago savijenih štapićastih mikroorganizama u apikalnoj citoplazmi enterocita. Imunohistohemijskim ispitivanjem upotrebom monoklonskog antitela protiv L. intracellularis potvrđeno je prisustvo mikroorganizama u apikalnoj citoplazmi hiperplastičnih enterocita i u lamini propriji. Dijagnoza hemoragične proliferativne enteropatije je postavljena na osnovu karakterističnih histoloških lezija i korišćenjem Warthin-Starry bojenja, kao i upotrebom imunohistohemijske metode, korišćenjem monoklonskog antitela protiv L. intracellularis. Imunohistohemijska metoda i Warthin-Starry metod bojenja mogu biti komplementarne metode za potvrdu infekcije izazvane sa L. intracellularis kod svinja. ., In this paper seven samples of the intestines originated from the naturally infected pigs, macroscopic, histochemically and immunohistochemically were examined. The caudal part of jejunum and ileum were affected. The affected intestine was thickened and dilated. The lumen of the ileum and colon contained one or more blood clots without feed contents. The rectum contained black tarry feces of mixed blood and digesta. The mucosal surface of the affected portion of intestine was markedly thickened without macroscopic erosions. Histological examination revealed: extensive degeneration, congestion, and hemorrhage within the proliferative epithelium, as well as a marked accumulation of the bloody cellular debris above affected mucosa. Clusters of argyrophilic, slightly curved rod-shaped microorganisms in the apical cytoplasm of enterocytes by Warthin-Starry silver stain were demonstrated. Immunohistochemical staining confirmed the presence of L. intracellularis in the apical cytoplasm of hyperplastic enterocytes and in lamina propria. In conclusion, diagnosis of hemorrhagic proliferative enteropathy is based on detection of the histologic lesions and detection of L. intracellularis by Warthin Starry silver stain, as well as by immunohistochemistry, where immunohistochemistry and Warthin-Starry silver method can be a complementary methods to confirm the diagnosis of L. intracellularis infection in pigs. .",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Savremena poljoprivreda",
title = "Patomorfološka dijagnostika hemoragične proliferativne enteropatije svinja, Patomorphological diagnosis of hemorrhagic proliferative enteropathy in swine",
pages = "74-68",
number = "1-2",
volume = "61",
url = "conv_20"
}
Kureljušić, B., Savić, B., Jezdimirović, N., Radanović, O., Kureljušić, J., Žutić, M.,& Ivetić, V.. (2012). Patomorfološka dijagnostika hemoragične proliferativne enteropatije svinja. in Savremena poljoprivreda
Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad., 61(1-2), 68-74.
conv_20
Kureljušić B, Savić B, Jezdimirović N, Radanović O, Kureljušić J, Žutić M, Ivetić V. Patomorfološka dijagnostika hemoragične proliferativne enteropatije svinja. in Savremena poljoprivreda. 2012;61(1-2):68-74.
conv_20 .
Kureljušić, Branislav, Savić, Božidar, Jezdimirović, Nemanja, Radanović, Oliver, Kureljušić, Jasna, Žutić, Milenko, Ivetić, Vojin, "Patomorfološka dijagnostika hemoragične proliferativne enteropatije svinja" in Savremena poljoprivreda, 61, no. 1-2 (2012):68-74,
conv_20 .

Enteritis associated with Clostridium perfringens type A in 9-month-old calves

Savić, Božidar; Prodanović, Radiša; Ivetić, Vojin; Radanović, Oliver; Bojkovski, Jovan

(2012)

TY  - JOUR
AU  - Savić, Božidar
AU  - Prodanović, Radiša
AU  - Ivetić, Vojin
AU  - Radanović, Oliver
AU  - Bojkovski, Jovan
PY  - 2012
UR  - https://reponivs.nivs.rs/handle/123456789/196
AB  - Four 9-month-old Simmental male calves were presented with a history of sudden death. The necropsy and microscopic findings allowed a diagnosis of enteritis and severe intraluminal hemorrhage with blood clots in the jejunum, suggestive of jejunal hemorrhage syndrome.
T2  - Canadian Veterinary Journal
T1  - Enteritis associated with Clostridium perfringens type A in 9-month-old calves
EP  - 176
IS  - 2
SP  - 174
VL  - 53
UR  - conv_686
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_887
ER  - 
@article{
author = "Savić, Božidar and Prodanović, Radiša and Ivetić, Vojin and Radanović, Oliver and Bojkovski, Jovan",
year = "2012",
abstract = "Four 9-month-old Simmental male calves were presented with a history of sudden death. The necropsy and microscopic findings allowed a diagnosis of enteritis and severe intraluminal hemorrhage with blood clots in the jejunum, suggestive of jejunal hemorrhage syndrome.",
journal = "Canadian Veterinary Journal",
title = "Enteritis associated with Clostridium perfringens type A in 9-month-old calves",
pages = "176-174",
number = "2",
volume = "53",
url = "conv_686, https://hdl.handle.net/21.15107/rcub_veterinar_887"
}
Savić, B., Prodanović, R., Ivetić, V., Radanović, O.,& Bojkovski, J.. (2012). Enteritis associated with Clostridium perfringens type A in 9-month-old calves. in Canadian Veterinary Journal, 53(2), 174-176.
conv_686
Savić B, Prodanović R, Ivetić V, Radanović O, Bojkovski J. Enteritis associated with Clostridium perfringens type A in 9-month-old calves. in Canadian Veterinary Journal. 2012;53(2):174-176.
conv_686 .
Savić, Božidar, Prodanović, Radiša, Ivetić, Vojin, Radanović, Oliver, Bojkovski, Jovan, "Enteritis associated with Clostridium perfringens type A in 9-month-old calves" in Canadian Veterinary Journal, 53, no. 2 (2012):174-176,
conv_686 .
7
11

Pojava aspergiloze u jatu ćurića

Kureljušić, Branislav; Radanović, Oliver; Kureljušić, Jasna; Jezdimirović, Nemanja; Maslić-Strižak, Danka; Prodanović, Radiša; Ivetić, Vojin

(Institut za stočarstvo, Beograd, 2012)

TY  - JOUR
AU  - Kureljušić, Branislav
AU  - Radanović, Oliver
AU  - Kureljušić, Jasna
AU  - Jezdimirović, Nemanja
AU  - Maslić-Strižak, Danka
AU  - Prodanović, Radiša
AU  - Ivetić, Vojin
PY  - 2012
UR  - https://reponivs.nivs.rs/handle/123456789/214
AB  - Aspergiloza je često gljivično oboljenje različitih vrsta ptica i sisara, izazvana gljivicama roda Aspergillus. Oboljenje se karakteriše inflamatornim promenama u respiratornom sistemu, a ponekad može da se javi u generalizovanoj formi kada je zahvaćeno više organskih sistema. U ovom radu, ispitano je jato ćurića, starosti 21 dan, na jednoj farmi u Srbiji. Kliničkim pregledom jata ustanovljeni su simptomi oboljenja centralnog nervnog sistema u formi ataksije, tortikolisa, pareze i paralize nogu i krila. Stopa mortaliteta u jatu je bila 7,2 %. Kod 10 od 12 obdukovanih ćurića na plućima su ustanovljeni multipli žuto-beli granulomi, od jedan do tri milimetara u prečniku. Kod 9 od 12 obdukovanih ćurića na sagitalnom preseku malog ili velikog mozga nađeni su pojedinačni žuto-beli granulomi, veličine od tri do pet milimetara u prečniku. Mikološkim ispitivanjem izolovana je gljivica Aspergillus fumigatus. Za procenu histopatoloških promena u plućima i mozgu kao i dokaz hifa gljivica, korišćene su tri histohemijske metode: hematoksilin-eozin (HE), Grocott methenamine silver (GMS) i PAS metoda. Mikroskopskim pregledom uzoraka pluća i mozga bojenih HE metodom, ustanovljeno je prisustvo granuloma sa centralnim područjem kazeozne nekroze koje je bilo okruženo sa gigantskim ćelijama, makrofagima, heterofilima, limfocitima i spoljašnjom kapsulom sačinjenom od vezivnog tkiva. Hife gljivica, kao sastavni delovi granuloma, bile su teško uočljive, a u nekim uzorcima bojenim HE metodom nisu se ni videle. Nasuprot ovome, u preparatima bojenim Grocott i PAS metodom jasno su se isticale septirane i razgranate hife u centralnim delovima granuloma. U dijagnostici ovog gljivičnog oboljenja neophodno je primeniti više histohemijskih metoda kako za procenu histopatoloških promena tako i za dokaz uzročnika, uključujući i izolaciju u cilju postavljanja etiološke dijagnoze.
AB  - Aspergillosis is frequent fungal disease of different avian and mammal species, caused by infection by the fungi of genus Aspergillus. The disease is characterized by inflammatory changes in the respiratory system and sometimes has generalized onset when more organ systems are affected. In this paper, we examined a flock of turkey poults, 21 days old, at one farm in Serbia. Clinical signs of central nervous system in the form of ataxia, torticollis, paresis and paralysis of legs and wings were observed. The mortality rate in the flock was 7,2 %. In ten out of twelve necropsied turkey poults multiple yellowish-white granulomas, one to three millimeters in diameter on lungs were found. In nine out of twelve necropsied turkey poults solitary yellowish-white granuloma, three to five millimeters in diameter on sagital section of the cerebrum or cerebellum were found. Mycological finding revealed fungi Aspergillus fumigatus. For the evaluation of histopathological changes in lung and brain and demonstration of fungal hyphae, three stain methods were used: haematoxylin-eosin (HE), Grocott methenamine silver and periodic acid Schiff (PAS) method. Microscopic examination of lung and brain has revealed the presence of granulomatous foci and caseous necrosis with surrounding region of proliferation including giant cells, macrophages, heterophils and lymphocytes and outer capsule of connective tissue. The fungal hyphae were hardly or not visible in HE stained sections, while septed and arborized hyphae were easily demonstrated by Grocott and PAS stain predominantly in central parts of granuloma. For diagnostic of mycotic infection is necessary to use different histochemical methods for evaluation of histopathological changes and detection of etiological agent, including isolation to obtain etiological diagnosis.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Pojava aspergiloze u jatu ćurića
T1  - The occurrence of aspergillosis in flock of turkey poults
EP  - 136
IS  - 1
SP  - 129
VL  - 28
DO  - 10.2298/BAH1201129K
UR  - conv_299
ER  - 
@article{
author = "Kureljušić, Branislav and Radanović, Oliver and Kureljušić, Jasna and Jezdimirović, Nemanja and Maslić-Strižak, Danka and Prodanović, Radiša and Ivetić, Vojin",
year = "2012",
abstract = "Aspergiloza je često gljivično oboljenje različitih vrsta ptica i sisara, izazvana gljivicama roda Aspergillus. Oboljenje se karakteriše inflamatornim promenama u respiratornom sistemu, a ponekad može da se javi u generalizovanoj formi kada je zahvaćeno više organskih sistema. U ovom radu, ispitano je jato ćurića, starosti 21 dan, na jednoj farmi u Srbiji. Kliničkim pregledom jata ustanovljeni su simptomi oboljenja centralnog nervnog sistema u formi ataksije, tortikolisa, pareze i paralize nogu i krila. Stopa mortaliteta u jatu je bila 7,2 %. Kod 10 od 12 obdukovanih ćurića na plućima su ustanovljeni multipli žuto-beli granulomi, od jedan do tri milimetara u prečniku. Kod 9 od 12 obdukovanih ćurića na sagitalnom preseku malog ili velikog mozga nađeni su pojedinačni žuto-beli granulomi, veličine od tri do pet milimetara u prečniku. Mikološkim ispitivanjem izolovana je gljivica Aspergillus fumigatus. Za procenu histopatoloških promena u plućima i mozgu kao i dokaz hifa gljivica, korišćene su tri histohemijske metode: hematoksilin-eozin (HE), Grocott methenamine silver (GMS) i PAS metoda. Mikroskopskim pregledom uzoraka pluća i mozga bojenih HE metodom, ustanovljeno je prisustvo granuloma sa centralnim područjem kazeozne nekroze koje je bilo okruženo sa gigantskim ćelijama, makrofagima, heterofilima, limfocitima i spoljašnjom kapsulom sačinjenom od vezivnog tkiva. Hife gljivica, kao sastavni delovi granuloma, bile su teško uočljive, a u nekim uzorcima bojenim HE metodom nisu se ni videle. Nasuprot ovome, u preparatima bojenim Grocott i PAS metodom jasno su se isticale septirane i razgranate hife u centralnim delovima granuloma. U dijagnostici ovog gljivičnog oboljenja neophodno je primeniti više histohemijskih metoda kako za procenu histopatoloških promena tako i za dokaz uzročnika, uključujući i izolaciju u cilju postavljanja etiološke dijagnoze., Aspergillosis is frequent fungal disease of different avian and mammal species, caused by infection by the fungi of genus Aspergillus. The disease is characterized by inflammatory changes in the respiratory system and sometimes has generalized onset when more organ systems are affected. In this paper, we examined a flock of turkey poults, 21 days old, at one farm in Serbia. Clinical signs of central nervous system in the form of ataxia, torticollis, paresis and paralysis of legs and wings were observed. The mortality rate in the flock was 7,2 %. In ten out of twelve necropsied turkey poults multiple yellowish-white granulomas, one to three millimeters in diameter on lungs were found. In nine out of twelve necropsied turkey poults solitary yellowish-white granuloma, three to five millimeters in diameter on sagital section of the cerebrum or cerebellum were found. Mycological finding revealed fungi Aspergillus fumigatus. For the evaluation of histopathological changes in lung and brain and demonstration of fungal hyphae, three stain methods were used: haematoxylin-eosin (HE), Grocott methenamine silver and periodic acid Schiff (PAS) method. Microscopic examination of lung and brain has revealed the presence of granulomatous foci and caseous necrosis with surrounding region of proliferation including giant cells, macrophages, heterophils and lymphocytes and outer capsule of connective tissue. The fungal hyphae were hardly or not visible in HE stained sections, while septed and arborized hyphae were easily demonstrated by Grocott and PAS stain predominantly in central parts of granuloma. For diagnostic of mycotic infection is necessary to use different histochemical methods for evaluation of histopathological changes and detection of etiological agent, including isolation to obtain etiological diagnosis.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Pojava aspergiloze u jatu ćurića, The occurrence of aspergillosis in flock of turkey poults",
pages = "136-129",
number = "1",
volume = "28",
doi = "10.2298/BAH1201129K",
url = "conv_299"
}
Kureljušić, B., Radanović, O., Kureljušić, J., Jezdimirović, N., Maslić-Strižak, D., Prodanović, R.,& Ivetić, V.. (2012). Pojava aspergiloze u jatu ćurića. in Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 28(1), 129-136.
https://doi.org/10.2298/BAH1201129K
conv_299
Kureljušić B, Radanović O, Kureljušić J, Jezdimirović N, Maslić-Strižak D, Prodanović R, Ivetić V. Pojava aspergiloze u jatu ćurića. in Biotechnology in Animal Husbandry. 2012;28(1):129-136.
doi:10.2298/BAH1201129K
conv_299 .
Kureljušić, Branislav, Radanović, Oliver, Kureljušić, Jasna, Jezdimirović, Nemanja, Maslić-Strižak, Danka, Prodanović, Radiša, Ivetić, Vojin, "Pojava aspergiloze u jatu ćurića" in Biotechnology in Animal Husbandry, 28, no. 1 (2012):129-136,
https://doi.org/10.2298/BAH1201129K .,
conv_299 .
1

Dermatitis tabanskih jastučića kod komercijalnih brojlera

Miljković, B.; Škrbić, Z.; Pavlovski, Zlatica; Lukić, Miloš; Ivetić, Vojin; Kureljušić, Branislav; Petričević, V.

(Institut za stočarstvo, Beograd, 2012)

TY  - JOUR
AU  - Miljković, B.
AU  - Škrbić, Z.
AU  - Pavlovski, Zlatica
AU  - Lukić, Miloš
AU  - Ivetić, Vojin
AU  - Kureljušić, Branislav
AU  - Petričević, V.
PY  - 2012
UR  - https://reponivs.nivs.rs/handle/123456789/216
AB  - U ogledu je ispitivano prisustvo mikoloških agenasa kod pilića sa lezijama na koži tabanskih jastučića, u cilju poboljšanja zdravlja brojlerskih pilica. Dermatitis na tabanskim jastučićima (DTJ) je u literaturi multifaktornog porekla. Postojanje iritantnih hemijskih supstanci u prostirci, genetske predispozicije hibrida, imunosupresivne bolesti pilića, kao i sastav komercijalnih smeša za ishranu brojlera koje utiču na pojavu vlažne prostirke, su dokazani faktori koji doprinose intenzivnijoj pojavi DTJ. Imajući u vidu ove podatke iz literature, pilići koji se gaje u zatvorenom prostoru u kome su prisutni bakterijski i mikološki agensi delom u prostirci ako nije na adekvatan način dezinfikovana, u hrani ili na perju pilića, želeli smo da ispitamo njihovo prisustvo u koži tabanskih jastučića. Ispitivanje je bazirano na pojavi DTJ u ogledu 500 jednodnevnih pilića Hubbard genotipa, koji su hranjeni na 2 načina (standardnim/ekonomičnim) krmnim smešama koje su prisutne na našem tržištu. Seckana slama je korišćena kao prostirka. DTJ se karakteriše lezijama na metatarzalnim i digitalnim ventralnim delovima kože tabana živine. To je kontaktni dermatitis koji kod težih slučajeva utiče na prirast, zdravlje i dobrobit. Na kraju tova pilića, svi pilići su pregledani na prisustvo lezija na koži tabana. U ovom radu, ispitano je 39 pilića sa intenzitetom lezija na tabanskim jastučićima. Intenzitet lezija je ocenjen sa 2 (blage) i sa 3 (izražene) lezije. Mikološkim ispitivanjem utvrđeno je prisustvo gljivice/plesni Aspergillus fumigatus, kod 9 autoklaviranih promenjenih pilećih nogica. Histološkim ispitivanjem utvrdili smo postojanje hiperkeratoze, panikulitisa, zapaljenje dermisa i balonirajuću degeneraciju. Nismo utvrdili prisustvo fungalnih elemenata u koži tabana, nakon bojenja Gomory methenamin silver bojenjem. Ptice su posebno osetljive na infekciju A. fumigatus koji je klasifikovan kao alergijski uzročnik.
AB  - In the present experiment, the presence of mycological agents in chickens with skin lesions on foot pads was investigated, in order to improve the health of broiler chickens. Foot-pad dermatitis (FPD) is described in the literature as dermatitis caused by multifactor causes. The presence of irritating chemical substances in the litter, genetic predispositions of hybrids, immune suppressive diseases of chickens, as well as the composition of commercial broiler diets that influence the occurrence of wet litter, are proven factors that contribute to a more intensive incidence of foot-pad dermatitis. Foot-pad dermatitis is a condition characterized by lesions on metatarsal and digital ventral skin of poultry feet. It is type of contact dermatitis which in severe cases shows slower weight gain, poor health condition and welfare. Taking into consideration the literature data, chickens reared in closed facilities where the bacterial and mycological agents are present partly in the litter if not properly disinfected, but also in the food or the chicken plumage, we wanted to investigate their presence in the skin of foot pads. The study was focused on incidence of foot-pad dermatitis in 500 one day old broiler chickens of Hubbard genotype, in two feeding (standard/economical) programs for broilers available on our market. Chopped straw was used as litter. At the end of fattening, all chickens were examined for the presence of lesions on the skin of the feet. Total of 39 chickens were allocated to the intensity of the lesion on foot pads rated as 2 (mild) and 3 (severe). In the mycological examination of the 9 samples of autoclaved chicken legs with lesions on the feet, we have isolated and identified the presence of fungi /mould Aspergillus fumigatus. The histopathology investigation, during which the tissue section from mild and severe skin foot pads was collected and stained using Hematoxylin and eosin, revealed the hyperkeratosis, panniculitis, ballooning degeneration and dermis inflammation. No fungal elements were observed in the sections of pads stained with Gomori methenamine silver. Birds are especially susceptible to infection with Aspergillus fumigatus. That species of fungus is also classified as allergenic factor.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Dermatitis tabanskih jastučića kod komercijalnih brojlera
T1  - Foot-pad dermatitis in commercial broilers
EP  - 843
IS  - 4
SP  - 835
VL  - 28
DO  - 10.2298/BAH1204835M
UR  - conv_302
ER  - 
@article{
author = "Miljković, B. and Škrbić, Z. and Pavlovski, Zlatica and Lukić, Miloš and Ivetić, Vojin and Kureljušić, Branislav and Petričević, V.",
year = "2012",
abstract = "U ogledu je ispitivano prisustvo mikoloških agenasa kod pilića sa lezijama na koži tabanskih jastučića, u cilju poboljšanja zdravlja brojlerskih pilica. Dermatitis na tabanskim jastučićima (DTJ) je u literaturi multifaktornog porekla. Postojanje iritantnih hemijskih supstanci u prostirci, genetske predispozicije hibrida, imunosupresivne bolesti pilića, kao i sastav komercijalnih smeša za ishranu brojlera koje utiču na pojavu vlažne prostirke, su dokazani faktori koji doprinose intenzivnijoj pojavi DTJ. Imajući u vidu ove podatke iz literature, pilići koji se gaje u zatvorenom prostoru u kome su prisutni bakterijski i mikološki agensi delom u prostirci ako nije na adekvatan način dezinfikovana, u hrani ili na perju pilića, želeli smo da ispitamo njihovo prisustvo u koži tabanskih jastučića. Ispitivanje je bazirano na pojavi DTJ u ogledu 500 jednodnevnih pilića Hubbard genotipa, koji su hranjeni na 2 načina (standardnim/ekonomičnim) krmnim smešama koje su prisutne na našem tržištu. Seckana slama je korišćena kao prostirka. DTJ se karakteriše lezijama na metatarzalnim i digitalnim ventralnim delovima kože tabana živine. To je kontaktni dermatitis koji kod težih slučajeva utiče na prirast, zdravlje i dobrobit. Na kraju tova pilića, svi pilići su pregledani na prisustvo lezija na koži tabana. U ovom radu, ispitano je 39 pilića sa intenzitetom lezija na tabanskim jastučićima. Intenzitet lezija je ocenjen sa 2 (blage) i sa 3 (izražene) lezije. Mikološkim ispitivanjem utvrđeno je prisustvo gljivice/plesni Aspergillus fumigatus, kod 9 autoklaviranih promenjenih pilećih nogica. Histološkim ispitivanjem utvrdili smo postojanje hiperkeratoze, panikulitisa, zapaljenje dermisa i balonirajuću degeneraciju. Nismo utvrdili prisustvo fungalnih elemenata u koži tabana, nakon bojenja Gomory methenamin silver bojenjem. Ptice su posebno osetljive na infekciju A. fumigatus koji je klasifikovan kao alergijski uzročnik., In the present experiment, the presence of mycological agents in chickens with skin lesions on foot pads was investigated, in order to improve the health of broiler chickens. Foot-pad dermatitis (FPD) is described in the literature as dermatitis caused by multifactor causes. The presence of irritating chemical substances in the litter, genetic predispositions of hybrids, immune suppressive diseases of chickens, as well as the composition of commercial broiler diets that influence the occurrence of wet litter, are proven factors that contribute to a more intensive incidence of foot-pad dermatitis. Foot-pad dermatitis is a condition characterized by lesions on metatarsal and digital ventral skin of poultry feet. It is type of contact dermatitis which in severe cases shows slower weight gain, poor health condition and welfare. Taking into consideration the literature data, chickens reared in closed facilities where the bacterial and mycological agents are present partly in the litter if not properly disinfected, but also in the food or the chicken plumage, we wanted to investigate their presence in the skin of foot pads. The study was focused on incidence of foot-pad dermatitis in 500 one day old broiler chickens of Hubbard genotype, in two feeding (standard/economical) programs for broilers available on our market. Chopped straw was used as litter. At the end of fattening, all chickens were examined for the presence of lesions on the skin of the feet. Total of 39 chickens were allocated to the intensity of the lesion on foot pads rated as 2 (mild) and 3 (severe). In the mycological examination of the 9 samples of autoclaved chicken legs with lesions on the feet, we have isolated and identified the presence of fungi /mould Aspergillus fumigatus. The histopathology investigation, during which the tissue section from mild and severe skin foot pads was collected and stained using Hematoxylin and eosin, revealed the hyperkeratosis, panniculitis, ballooning degeneration and dermis inflammation. No fungal elements were observed in the sections of pads stained with Gomori methenamine silver. Birds are especially susceptible to infection with Aspergillus fumigatus. That species of fungus is also classified as allergenic factor.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Dermatitis tabanskih jastučića kod komercijalnih brojlera, Foot-pad dermatitis in commercial broilers",
pages = "843-835",
number = "4",
volume = "28",
doi = "10.2298/BAH1204835M",
url = "conv_302"
}
Miljković, B., Škrbić, Z., Pavlovski, Z., Lukić, M., Ivetić, V., Kureljušić, B.,& Petričević, V.. (2012). Dermatitis tabanskih jastučića kod komercijalnih brojlera. in Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 28(4), 835-843.
https://doi.org/10.2298/BAH1204835M
conv_302
Miljković B, Škrbić Z, Pavlovski Z, Lukić M, Ivetić V, Kureljušić B, Petričević V. Dermatitis tabanskih jastučića kod komercijalnih brojlera. in Biotechnology in Animal Husbandry. 2012;28(4):835-843.
doi:10.2298/BAH1204835M
conv_302 .
Miljković, B., Škrbić, Z., Pavlovski, Zlatica, Lukić, Miloš, Ivetić, Vojin, Kureljušić, Branislav, Petričević, V., "Dermatitis tabanskih jastučića kod komercijalnih brojlera" in Biotechnology in Animal Husbandry, 28, no. 4 (2012):835-843,
https://doi.org/10.2298/BAH1204835M .,
conv_302 .
3

Uloga Harderove žlezde u imunom odgovoru pilića sa maternalnim imunitetom na vakcinu protiv virusnog infektivnog bronhitisa

Spalević, Ljiljana; Ivetić, Vojin; Jakić-Dimić, Dobrila; Maslić-Strižak, Danka; Potkonjak, Aleksandar; Pavlović, Nikola

(Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd, 2012)

TY  - JOUR
AU  - Spalević, Ljiljana
AU  - Ivetić, Vojin
AU  - Jakić-Dimić, Dobrila
AU  - Maslić-Strižak, Danka
AU  - Potkonjak, Aleksandar
AU  - Pavlović, Nikola
PY  - 2012
UR  - https://reponivs.nivs.rs/handle/123456789/193
AB  - U ovom radu je ispitivana uloga Harderove žlezde u imunološkom odgovoru nakon vakcinacije protiv infektivnog bronhitisa (IB). Ogledom je obuhvaćeno 100 brojlerskih pilića koji su podeljeni u dve grupe. Ogledna grupa vakcinisana je prvog dana starosti protiv infektivnog bronhitisa vakcinom Bronhivet I, koja sadrži živi atenuirani soj H120. Kontrolna grupa nije imunizovana. Ogled je trajao 21 dan. Ispitivani su krvni serumi 1, 7, 14. i 21. dana na prisustvo specifičnih antitela prema infektivnom bronhitisu (ELISA test). Praćene su histološke promene u građi Harderove žlezde u odnosu na primenjenu vakcinu. Ovim istraživanjem je dokazana uloga Harderove žlezde kao sekundarnog limfoidnog organa kod vakcinacije brojlerskih pilića protiv infektivnog bronhitisa. Prisutna maternalna antitela na IB nemaju negativan uticaj na vakcinaciju pilića prvog dana starosti. Vakcinalni virus delimično oštećuje Harderovu žlezdu.
AB  - In this study we investigated the role of the Harderian gland in the immune response following vaccination against infectious bronchitis (IB). The experiment was carried out on 100 broiler chicks which were divided into two experimental groups. Experimental group O was vaccinated on the first day of age against infectious bronchitis with vaccine Bronhivet I batches that contain a live attenuated strain H120 and the control group did not get immunized. The experiment lasted 21 days. Blood sera were examined on days 1, 7, 14 and 21 for the presence of specific antibodies against infectious bronchitis (ELISA test). Histological changes were observed in the structure of the Harderian gland in relation to the applied vaccine. This research demonstrated the role of the Harderian gland as a secondary lymphoid organ in broiler vaccination against infectious bronchitis. Maternal antibodies present in the IB had no negative impact on the vaccination of chickens on the first day of age. The vaccine virus partially damaged the Harderian gland.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski glasnik
T1  - Uloga Harderove žlezde u imunom odgovoru pilića sa maternalnim imunitetom na vakcinu protiv virusnog infektivnog bronhitisa
T1  - Role of Harderian gland in immune response of chickens with maternal immunity to vaccine against infectious bronchitis virus
EP  - 218
IS  - 3-4
SP  - 211
VL  - 66
DO  - 10.2298/VETGL1204211S
UR  - conv_94
ER  - 
@article{
author = "Spalević, Ljiljana and Ivetić, Vojin and Jakić-Dimić, Dobrila and Maslić-Strižak, Danka and Potkonjak, Aleksandar and Pavlović, Nikola",
year = "2012",
abstract = "U ovom radu je ispitivana uloga Harderove žlezde u imunološkom odgovoru nakon vakcinacije protiv infektivnog bronhitisa (IB). Ogledom je obuhvaćeno 100 brojlerskih pilića koji su podeljeni u dve grupe. Ogledna grupa vakcinisana je prvog dana starosti protiv infektivnog bronhitisa vakcinom Bronhivet I, koja sadrži živi atenuirani soj H120. Kontrolna grupa nije imunizovana. Ogled je trajao 21 dan. Ispitivani su krvni serumi 1, 7, 14. i 21. dana na prisustvo specifičnih antitela prema infektivnom bronhitisu (ELISA test). Praćene su histološke promene u građi Harderove žlezde u odnosu na primenjenu vakcinu. Ovim istraživanjem je dokazana uloga Harderove žlezde kao sekundarnog limfoidnog organa kod vakcinacije brojlerskih pilića protiv infektivnog bronhitisa. Prisutna maternalna antitela na IB nemaju negativan uticaj na vakcinaciju pilića prvog dana starosti. Vakcinalni virus delimično oštećuje Harderovu žlezdu., In this study we investigated the role of the Harderian gland in the immune response following vaccination against infectious bronchitis (IB). The experiment was carried out on 100 broiler chicks which were divided into two experimental groups. Experimental group O was vaccinated on the first day of age against infectious bronchitis with vaccine Bronhivet I batches that contain a live attenuated strain H120 and the control group did not get immunized. The experiment lasted 21 days. Blood sera were examined on days 1, 7, 14 and 21 for the presence of specific antibodies against infectious bronchitis (ELISA test). Histological changes were observed in the structure of the Harderian gland in relation to the applied vaccine. This research demonstrated the role of the Harderian gland as a secondary lymphoid organ in broiler vaccination against infectious bronchitis. Maternal antibodies present in the IB had no negative impact on the vaccination of chickens on the first day of age. The vaccine virus partially damaged the Harderian gland.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski glasnik",
title = "Uloga Harderove žlezde u imunom odgovoru pilića sa maternalnim imunitetom na vakcinu protiv virusnog infektivnog bronhitisa, Role of Harderian gland in immune response of chickens with maternal immunity to vaccine against infectious bronchitis virus",
pages = "218-211",
number = "3-4",
volume = "66",
doi = "10.2298/VETGL1204211S",
url = "conv_94"
}
Spalević, L., Ivetić, V., Jakić-Dimić, D., Maslić-Strižak, D., Potkonjak, A.,& Pavlović, N.. (2012). Uloga Harderove žlezde u imunom odgovoru pilića sa maternalnim imunitetom na vakcinu protiv virusnog infektivnog bronhitisa. in Veterinarski glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 66(3-4), 211-218.
https://doi.org/10.2298/VETGL1204211S
conv_94
Spalević L, Ivetić V, Jakić-Dimić D, Maslić-Strižak D, Potkonjak A, Pavlović N. Uloga Harderove žlezde u imunom odgovoru pilića sa maternalnim imunitetom na vakcinu protiv virusnog infektivnog bronhitisa. in Veterinarski glasnik. 2012;66(3-4):211-218.
doi:10.2298/VETGL1204211S
conv_94 .
Spalević, Ljiljana, Ivetić, Vojin, Jakić-Dimić, Dobrila, Maslić-Strižak, Danka, Potkonjak, Aleksandar, Pavlović, Nikola, "Uloga Harderove žlezde u imunom odgovoru pilića sa maternalnim imunitetom na vakcinu protiv virusnog infektivnog bronhitisa" in Veterinarski glasnik, 66, no. 3-4 (2012):211-218,
https://doi.org/10.2298/VETGL1204211S .,
conv_94 .

Primena različitih histohemijskih metoda u dijagnostici Aspergiloze mozga kod ćurića

Kureljušić, Branislav; Savić, Božidar; Prodanović, Radiša; Đekić, Jovo; Adamov, Vladica; Jakić-Dimić, Dobrila; Miljković, Biljana; Radanović, Oliver; Ivetić, Vojin

(Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd, 2011)

TY  - JOUR
AU  - Kureljušić, Branislav
AU  - Savić, Božidar
AU  - Prodanović, Radiša
AU  - Đekić, Jovo
AU  - Adamov, Vladica
AU  - Jakić-Dimić, Dobrila
AU  - Miljković, Biljana
AU  - Radanović, Oliver
AU  - Ivetić, Vojin
PY  - 2011
UR  - https://reponivs.nivs.rs/handle/123456789/182
AB  - Aspergiloza je često gljivično oboljenje različitih vrsta ptica i sisara, izazvano gljivicama roda Aspergillus. Karakteriše se inflamatornim promenama, pre svega, u respiratornom sistemu, mada ponekad protiče i u generalizovanoj formi kada je afektirano više organskih sistema. Kod ćuraka, pataka i gusaka opisan je mikotični granulomatozni meningoencefalitis sa pretežnom lokalizacijom u malom mozgu. U radu je ispitano jato tovnih ćurića starosti 12 dana, kod kojih su se pojavile neurološke smetnje u vidu ataksije, tortikolisa, pareze i paralize zadnjih ekstermiteta i krila. Kod tri od deset obdukovanih ćurića makroskopski nalaz je ukazao na granulomatozni encefalitis malog mozga. Granulom bele boje, prečnika oko 3 mm, bio je situiran kranioventralno i jasno se uočavao na sagitalnom preseku malog mozga. Mikološkim ispitivanjem lezije iz malog mozga izolovana je gljivica Aspergillus fumigatus. Za procenu histopatoloških promena i dokaz hifa Aspergillus fumigatusa korišćene su hematoksilin-eozin (HE), Grocott i PAS metode. Mikroskopskim pregledom isečaka tkiva mozga bojenih HE metodom zapaženo je postojanje granuloma sa centralno postavljenim nekrotičnim poljem. Polja nekroze su infiltrovana heterofilnim granulocitima i okolo okružena makrofagima, džinovskim ćelijama i limfocitima. Na periferiji granuloma se nalazi vezivno-tkivna kapsula. Hife gljivica, kao sastavni delovi granuloma, bile su teško uočljive, a u nekim uzorcima bojenim HE metodom nisu se ni videle. Nasuprot ovome, u preparatima bojenim Grocott i PAS metodom jasno su se isticale septirane i razgranate hife u različitim delovima granuloma. U cilju postavljanja etiološke dijagnoze mikotičnih oboljenja neophodno je pored izolacije primeniti i više histohemijskih metoda.
AB  - Aspergillosis is a frequent fungal disease in different species of birds and mammals caused by fungi of the genus Aspergillus. It is characterized by inflammatory changes primarily in the respiratory system, even though it sometimes takes on a generalized form when several organ systems are affected. Mucotic-granulomatous meningoencephalitis with a predominant localization in the cerebellum has been described in turkeys, ducks and geese. Within this paper, examinations have been performed on a flock of broiler turkeys aged 12 days who had sustained evident neurological disorders in the form of ataxy, torticollis, paresis, and paralysis of the hind extremities and wings. In three of the ten autopsied chicks the macroscopic findings indicated granulomatous encephalitis of the cerebellum. A white-coloured granuloma, around 3mm in diameter, was situated cranioventrally and was clearly visible on the sagital section of the cerebellum. Mucological examinations of the cerebellum lesion resulted in the isolation of the fungi Aspergillus fumigatus. Haematoxylin-eosin (HE), Grocott and PAS methods were used for the evaluation of histopathological changes and proving Aspergillus fumigatusa hyphae. The microscopic examination of brain tissue sections stained with the HE method revealed the existence of a granuloma with a centrally placed necrotic area. The necrotic area was infiltrated with heterophilic granulocytes and surrounded by macrophage, giant cells and lymphocytes. A connective tissue capsule was located on the periphery of the granuloma. The fungi hyphae, as integral parts of the granuloma, were difficult to observe, and in some samples stained using the HE method they could not be seen at all. On the other hand, sections stanied using the Grocott and PAS methods showed prominent septed and branched hyphae in different parts of the granuloma. With the objective of making an etiological diagnosis of mucotic diseases, it is necessary to apply several histochemical methods, in addition to isolation.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski glasnik
T1  - Primena različitih histohemijskih metoda u dijagnostici Aspergiloze mozga kod ćurića
T1  - Implementation of different histochemical methods in diagnostics of brain Aspergillosis in turkey chicks
EP  - 49
IS  - 1-2
SP  - 43
VL  - 65
DO  - 10.2298/VETGL1102043K
UR  - conv_86
ER  - 
@article{
author = "Kureljušić, Branislav and Savić, Božidar and Prodanović, Radiša and Đekić, Jovo and Adamov, Vladica and Jakić-Dimić, Dobrila and Miljković, Biljana and Radanović, Oliver and Ivetić, Vojin",
year = "2011",
abstract = "Aspergiloza je često gljivično oboljenje različitih vrsta ptica i sisara, izazvano gljivicama roda Aspergillus. Karakteriše se inflamatornim promenama, pre svega, u respiratornom sistemu, mada ponekad protiče i u generalizovanoj formi kada je afektirano više organskih sistema. Kod ćuraka, pataka i gusaka opisan je mikotični granulomatozni meningoencefalitis sa pretežnom lokalizacijom u malom mozgu. U radu je ispitano jato tovnih ćurića starosti 12 dana, kod kojih su se pojavile neurološke smetnje u vidu ataksije, tortikolisa, pareze i paralize zadnjih ekstermiteta i krila. Kod tri od deset obdukovanih ćurića makroskopski nalaz je ukazao na granulomatozni encefalitis malog mozga. Granulom bele boje, prečnika oko 3 mm, bio je situiran kranioventralno i jasno se uočavao na sagitalnom preseku malog mozga. Mikološkim ispitivanjem lezije iz malog mozga izolovana je gljivica Aspergillus fumigatus. Za procenu histopatoloških promena i dokaz hifa Aspergillus fumigatusa korišćene su hematoksilin-eozin (HE), Grocott i PAS metode. Mikroskopskim pregledom isečaka tkiva mozga bojenih HE metodom zapaženo je postojanje granuloma sa centralno postavljenim nekrotičnim poljem. Polja nekroze su infiltrovana heterofilnim granulocitima i okolo okružena makrofagima, džinovskim ćelijama i limfocitima. Na periferiji granuloma se nalazi vezivno-tkivna kapsula. Hife gljivica, kao sastavni delovi granuloma, bile su teško uočljive, a u nekim uzorcima bojenim HE metodom nisu se ni videle. Nasuprot ovome, u preparatima bojenim Grocott i PAS metodom jasno su se isticale septirane i razgranate hife u različitim delovima granuloma. U cilju postavljanja etiološke dijagnoze mikotičnih oboljenja neophodno je pored izolacije primeniti i više histohemijskih metoda., Aspergillosis is a frequent fungal disease in different species of birds and mammals caused by fungi of the genus Aspergillus. It is characterized by inflammatory changes primarily in the respiratory system, even though it sometimes takes on a generalized form when several organ systems are affected. Mucotic-granulomatous meningoencephalitis with a predominant localization in the cerebellum has been described in turkeys, ducks and geese. Within this paper, examinations have been performed on a flock of broiler turkeys aged 12 days who had sustained evident neurological disorders in the form of ataxy, torticollis, paresis, and paralysis of the hind extremities and wings. In three of the ten autopsied chicks the macroscopic findings indicated granulomatous encephalitis of the cerebellum. A white-coloured granuloma, around 3mm in diameter, was situated cranioventrally and was clearly visible on the sagital section of the cerebellum. Mucological examinations of the cerebellum lesion resulted in the isolation of the fungi Aspergillus fumigatus. Haematoxylin-eosin (HE), Grocott and PAS methods were used for the evaluation of histopathological changes and proving Aspergillus fumigatusa hyphae. The microscopic examination of brain tissue sections stained with the HE method revealed the existence of a granuloma with a centrally placed necrotic area. The necrotic area was infiltrated with heterophilic granulocytes and surrounded by macrophage, giant cells and lymphocytes. A connective tissue capsule was located on the periphery of the granuloma. The fungi hyphae, as integral parts of the granuloma, were difficult to observe, and in some samples stained using the HE method they could not be seen at all. On the other hand, sections stanied using the Grocott and PAS methods showed prominent septed and branched hyphae in different parts of the granuloma. With the objective of making an etiological diagnosis of mucotic diseases, it is necessary to apply several histochemical methods, in addition to isolation.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski glasnik",
title = "Primena različitih histohemijskih metoda u dijagnostici Aspergiloze mozga kod ćurića, Implementation of different histochemical methods in diagnostics of brain Aspergillosis in turkey chicks",
pages = "49-43",
number = "1-2",
volume = "65",
doi = "10.2298/VETGL1102043K",
url = "conv_86"
}
Kureljušić, B., Savić, B., Prodanović, R., Đekić, J., Adamov, V., Jakić-Dimić, D., Miljković, B., Radanović, O.,& Ivetić, V.. (2011). Primena različitih histohemijskih metoda u dijagnostici Aspergiloze mozga kod ćurića. in Veterinarski glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 65(1-2), 43-49.
https://doi.org/10.2298/VETGL1102043K
conv_86
Kureljušić B, Savić B, Prodanović R, Đekić J, Adamov V, Jakić-Dimić D, Miljković B, Radanović O, Ivetić V. Primena različitih histohemijskih metoda u dijagnostici Aspergiloze mozga kod ćurića. in Veterinarski glasnik. 2011;65(1-2):43-49.
doi:10.2298/VETGL1102043K
conv_86 .
Kureljušić, Branislav, Savić, Božidar, Prodanović, Radiša, Đekić, Jovo, Adamov, Vladica, Jakić-Dimić, Dobrila, Miljković, Biljana, Radanović, Oliver, Ivetić, Vojin, "Primena različitih histohemijskih metoda u dijagnostici Aspergiloze mozga kod ćurića" in Veterinarski glasnik, 65, no. 1-2 (2011):43-49,
https://doi.org/10.2298/VETGL1102043K .,
conv_86 .

Paratuberkuloza u zapatu krava rase crveni holštajn

Prodanović, Radiša; Vujanac, Ivan; Kirovski, Danijela; Ivetić, Vojin; Savić, Božidar; Žutić, Milenko; Kureljušić, Branislav; Radanović, Oliver

(Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd, 2011)

TY  - JOUR
AU  - Prodanović, Radiša
AU  - Vujanac, Ivan
AU  - Kirovski, Danijela
AU  - Ivetić, Vojin
AU  - Savić, Božidar
AU  - Žutić, Milenko
AU  - Kureljušić, Branislav
AU  - Radanović, Oliver
PY  - 2011
UR  - https://reponivs.nivs.rs/handle/123456789/174
AB  - U ovom radu opisana je paratuberkuloza u izolovanom zapatu od 25 visokomlečnih krava rase crveni holštajn. Životinje u zapatu su klinič ki pregledane, a zatim podvrgnute testu kasne preosetljivosti i ispitivanju krvnog seruma na prisustvo specifičnih antitela protiv Mycobacterium avium subsp. paratuberculosis (Map). Kliničkim pregledom kod dve životinje uočeni su simptomi bolesti koji su ukazivali na uznapredovali stadijum paratuberkuloze. U krvi krava koje su pokazivale kliničke znake bolesti određivani su parametri crvene i bele krvne slike, koncentracija ukupnih proteina, albumina, gvožđa, natrijuma, kalijuma i aktivnost kreatin kinaze. Analizom parametara crvene krvne slike utvrđena su izvesna odstupanja koja su ukazivala na postojanje hipohromne mikrocitne anemije. Broj leukocita je bio u okviru fizioloških vrednosti, ali je neutrofilno-limfocitni količnik bio narušen i iznosio skoro 1:1. Rezultati biohemijskih analiza krvnog seruma obolelih krava ukazali su na hipoproteinemiju, hipoalbuminemiju, hipoferemiju, hiponatremiju, hipokalemiju i povećanu aktivnost enzima kreatin kinaze. Sumnjiva reakcija na mestu aplikacije avijarnog tuberkulina je ustanovljena kod dve životinje. Životinje sa kliničkim znakovima bolesti su bile negativne na test kasne preosetljivosti. Prisustvo specifičnih antitela protiv uzročnika paratuberkuloze dokazano je kod četiri životinje (16%), uključujući dve životinje sa kliničkim znakovima bolesti i jednu koja je imala sumnjivu reakciju na mestu aplikacija avijarnog tuberkulina. Pored toga, kod jedne uginule životinje opisane su makroskopske i mikroskopske promene u odnosu na intenzitet i distribuciju lezija, tip ćelijskog infiltrata i broja prisutnih acidorezistentnih bakterija, a promene su okarakterisane kao difuzne promene multibacilarnog tipa. Iz promenjenih organa izvršena je izolacija uzročnika bovine paratuberkuloze.
AB  - This paper describes paratuberculosis in an isolated breeding herd of 25 high-yield dairy cows of the Red Holstein breed. The animals were examined clinically and then given the test for ldelayed type hypersensitivity and their blood serum was examined for the presence of specific antibodies against Mycobacterium avium subsp. paratuberculosis (Map). The clinical examination revealed that two cows exhibited symptoms of the disease that indicated an advanced stage of paratuberculosis. The following parameters were examined in the blood of the cows that showed clinical signs of the disease: leukocytes and erythrocytes count, concentrations of total proteins, albumin, iron, sodium, potassium, and activity of creatine kinase. The analysis of the red blood cell count revealed certain digressions that indicated the existence of hypochromic microcytic anaemia. The number of leukocytes was within the physiological values, but the neutrophil-lymphocyte ratio was disrupted and stood at almost 1:1. The results of the biochemical analyses of the blood serum of diseased cows indicated hypoproteinaemia, hypoalbuminaemia, hypoferremia, hyposodiumaemia, hypokalemia, and increased activities of creatine kinase enzymes. A suspect reaction on the site of application of avian tuberculin was determined in two animals. Animals with clinical signs of the disease reacted negative to the test of delayed type hypersensitivity. The presence of specific antibodies against the cause of paratuberculosis was proven in four animals (16%), including two animals with clinical signs of the disease and one that had a suspect reaction on the site of application of avian tuberculin. Furthermore, one animal that died exhibited macroscopic and microscopic changes regarding the intensity and distribution of lesions, the type of cellular infiltrate, and the number of present acidresistent bacteria, and the changes were characterized as diffuse changes of multibacillary type. The cause of bovine paratuberculosis was isolated from the altered organs.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski glasnik
T1  - Paratuberkuloza u zapatu krava rase crveni holštajn
T1  - Paratuberculosis in breeding stock of red Holstein cows
EP  - 190
IS  - 3-4
SP  - 179
VL  - 65
DO  - 10.2298/VETGL1104179P
UR  - conv_88
ER  - 
@article{
author = "Prodanović, Radiša and Vujanac, Ivan and Kirovski, Danijela and Ivetić, Vojin and Savić, Božidar and Žutić, Milenko and Kureljušić, Branislav and Radanović, Oliver",
year = "2011",
abstract = "U ovom radu opisana je paratuberkuloza u izolovanom zapatu od 25 visokomlečnih krava rase crveni holštajn. Životinje u zapatu su klinič ki pregledane, a zatim podvrgnute testu kasne preosetljivosti i ispitivanju krvnog seruma na prisustvo specifičnih antitela protiv Mycobacterium avium subsp. paratuberculosis (Map). Kliničkim pregledom kod dve životinje uočeni su simptomi bolesti koji su ukazivali na uznapredovali stadijum paratuberkuloze. U krvi krava koje su pokazivale kliničke znake bolesti određivani su parametri crvene i bele krvne slike, koncentracija ukupnih proteina, albumina, gvožđa, natrijuma, kalijuma i aktivnost kreatin kinaze. Analizom parametara crvene krvne slike utvrđena su izvesna odstupanja koja su ukazivala na postojanje hipohromne mikrocitne anemije. Broj leukocita je bio u okviru fizioloških vrednosti, ali je neutrofilno-limfocitni količnik bio narušen i iznosio skoro 1:1. Rezultati biohemijskih analiza krvnog seruma obolelih krava ukazali su na hipoproteinemiju, hipoalbuminemiju, hipoferemiju, hiponatremiju, hipokalemiju i povećanu aktivnost enzima kreatin kinaze. Sumnjiva reakcija na mestu aplikacije avijarnog tuberkulina je ustanovljena kod dve životinje. Životinje sa kliničkim znakovima bolesti su bile negativne na test kasne preosetljivosti. Prisustvo specifičnih antitela protiv uzročnika paratuberkuloze dokazano je kod četiri životinje (16%), uključujući dve životinje sa kliničkim znakovima bolesti i jednu koja je imala sumnjivu reakciju na mestu aplikacija avijarnog tuberkulina. Pored toga, kod jedne uginule životinje opisane su makroskopske i mikroskopske promene u odnosu na intenzitet i distribuciju lezija, tip ćelijskog infiltrata i broja prisutnih acidorezistentnih bakterija, a promene su okarakterisane kao difuzne promene multibacilarnog tipa. Iz promenjenih organa izvršena je izolacija uzročnika bovine paratuberkuloze., This paper describes paratuberculosis in an isolated breeding herd of 25 high-yield dairy cows of the Red Holstein breed. The animals were examined clinically and then given the test for ldelayed type hypersensitivity and their blood serum was examined for the presence of specific antibodies against Mycobacterium avium subsp. paratuberculosis (Map). The clinical examination revealed that two cows exhibited symptoms of the disease that indicated an advanced stage of paratuberculosis. The following parameters were examined in the blood of the cows that showed clinical signs of the disease: leukocytes and erythrocytes count, concentrations of total proteins, albumin, iron, sodium, potassium, and activity of creatine kinase. The analysis of the red blood cell count revealed certain digressions that indicated the existence of hypochromic microcytic anaemia. The number of leukocytes was within the physiological values, but the neutrophil-lymphocyte ratio was disrupted and stood at almost 1:1. The results of the biochemical analyses of the blood serum of diseased cows indicated hypoproteinaemia, hypoalbuminaemia, hypoferremia, hyposodiumaemia, hypokalemia, and increased activities of creatine kinase enzymes. A suspect reaction on the site of application of avian tuberculin was determined in two animals. Animals with clinical signs of the disease reacted negative to the test of delayed type hypersensitivity. The presence of specific antibodies against the cause of paratuberculosis was proven in four animals (16%), including two animals with clinical signs of the disease and one that had a suspect reaction on the site of application of avian tuberculin. Furthermore, one animal that died exhibited macroscopic and microscopic changes regarding the intensity and distribution of lesions, the type of cellular infiltrate, and the number of present acidresistent bacteria, and the changes were characterized as diffuse changes of multibacillary type. The cause of bovine paratuberculosis was isolated from the altered organs.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski glasnik",
title = "Paratuberkuloza u zapatu krava rase crveni holštajn, Paratuberculosis in breeding stock of red Holstein cows",
pages = "190-179",
number = "3-4",
volume = "65",
doi = "10.2298/VETGL1104179P",
url = "conv_88"
}
Prodanović, R., Vujanac, I., Kirovski, D., Ivetić, V., Savić, B., Žutić, M., Kureljušić, B.,& Radanović, O.. (2011). Paratuberkuloza u zapatu krava rase crveni holštajn. in Veterinarski glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 65(3-4), 179-190.
https://doi.org/10.2298/VETGL1104179P
conv_88
Prodanović R, Vujanac I, Kirovski D, Ivetić V, Savić B, Žutić M, Kureljušić B, Radanović O. Paratuberkuloza u zapatu krava rase crveni holštajn. in Veterinarski glasnik. 2011;65(3-4):179-190.
doi:10.2298/VETGL1104179P
conv_88 .
Prodanović, Radiša, Vujanac, Ivan, Kirovski, Danijela, Ivetić, Vojin, Savić, Božidar, Žutić, Milenko, Kureljušić, Branislav, Radanović, Oliver, "Paratuberkuloza u zapatu krava rase crveni holštajn" in Veterinarski glasnik, 65, no. 3-4 (2011):179-190,
https://doi.org/10.2298/VETGL1104179P .,
conv_88 .

Uporedno ispitivanje efikasnosti tulatromicina i florfenikola u lečenju bronhopneumonije junadi

Jezdimirović, Milanka; Aleksić, Nevenka; Barać-Bašević, Ana; Žutić, Milenko; Ivetić, Vojin; Radanović, Oliver; Jezdimirović, Nemanja

(Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd, 2011)

TY  - JOUR
AU  - Jezdimirović, Milanka
AU  - Aleksić, Nevenka
AU  - Barać-Bašević, Ana
AU  - Žutić, Milenko
AU  - Ivetić, Vojin
AU  - Radanović, Oliver
AU  - Jezdimirović, Nemanja
PY  - 2011
UR  - https://reponivs.nivs.rs/handle/123456789/181
AB  - Ispitivana je klinička efikasnost tulatromicina (TU) i florfenikola (FL) u lečenju bronhopneumonije (BP) prouzrokovane vrstom Pasteurella multocida, izolovanom iz briseva nosa junadi. Simptomi BP su kvantifikovani da bi se dobio klinički skor (KS) koji definiše težinu kliničke slike kod životinja. Prema korišćenoj skali maksimalna vrednost KS parametara BP iznosi 47 bodova. U našem ogledu, vrednost prosečnog KS kod obolele junadi iznosila je 23,5±0,15. Klinička efikasnost TU i FL procenjivana je svakodnevno, tokom sedam dana, na osnovu povlačenja kliničkih simptoma (smanjivanje vrednosti KS) i prisustva prouzrokovača BP u brisevima nosa 7, 28. i 35. dana od ukidanja terapije. Tulatromicin je aplikovan s.c., jednokratno u terapijskoj dozi od 2,5 mg/kg, a florfenikol s.c., dvokratno u razmaku od 48 h u terapijskoj dozi od 40 mg/kg tm. U ovom ispitivanju TU je u prva četri dana od aplikacije značajno brže od FL (P LT 0,05) dovodio do povlačenja bitnih kliničkih simptoma, iako je aplikacija FL u tom periodu ponovljena. Klinička efikasnost TU u lečenju BP junadi četvrtog dana od primene iznosila je 43,4±1,5 %, a florfenikola 27,2±1,6 %. Međutim, petog dana od aplikacije TU i FL i drugog dana od druge aplikacije FL ustanovljena je približno ista klinička efikasnost ovih antimikrobnih lekova u lečenju BP. Prosečna efikasnost TU bila je 57,1±0,0%, a FL 58,5± 0,0%. Vrednost individualnog i prosečnog KS kod junadi lečene TU i FL iznosila je 10 bodova. Od kliničkih simptoma jedino se hipertermija nije povukla. Šestog dana od aplikacije TU i FL i trećeg dana od druge aplikacije FL oba antibiotika imala su podjednaku, maksimalnu (100%) kliničku efikasnost u lečenju BP. Ista klinička efikasnost zabeležena je i sedmog dana ogleda. Izlečenje je potvrđeno odsustvom P. multocida u brisevima uzetim sedmog dana od početka lečenja. Međutim, kod 4 juneta (21,05 %) od ukupno 19 izolovan je Streptococcus alpha haemolyticus. Četiri nedelje od obustavljanja primene TU i FL uočen je recidiv BP prouzrokovane P. multocida kod 30% junadi lečene TU i 22,22% lečene FL. Posle jednokratne primene TU i FL, sedmog dana su se povukli svi klinički simptomi recidivirajuće BP, a mikrobiološki nalaz bio je negativan. Sumirajući rezultate dobijene uporednim ispitivanjem efikasnosti TU i FL u lečenju BP junadi prouzrokovane P. multocida predlažemo da se TU koristi kao lek prvog izbora u lečenju ove respiratorne infekcije. Njegova efikasnost, nedovoljna u prva tri dana od aplikacije, može da se poveća istovremenom primenom lekova iz grupe NSAIL i bronhodilatatora.
AB  - The clinical efficacy of tulathromycin (TU) and florfenicol (FL) in the treatment of bronchopneumonia (BP) caused by Pasteurella multocida which was isolated from nose swabs of diseased calves has been examined. The symptoms of bronchopneumonia (BP) were quantified by means of the clinical score (CS) with a maximum of 47 points. In the current investigation the average CS in diseased calves was 23.5± 0.15. The clinical efficacy of TU and FL was assessed every day in the first week after the administration of the drugs and was based on the decrease in CS and on microbiological findings on days 7, 28 and 35 after the completion of therapy. Tulathromycin was administered s.c., in the prescribed therapeutic dose (2.5 mg/kg BW), and florfenicol s.c., twice at a 48 h interval, in its respective therapeutic dose (40 mg/kg BW). In spite of the repeated administration of FL, TU was significantly more rapid to decrease the major clinical symptoms in the first four days following the application, in comparison with FL (P LT 0.05). On the fourth day after the administration, the clinical efficacy of TU in the therapy of BP in calves was 43.4±1.5 %, and of florfenicol 27.2±1.6 %. However, five days after the application of TU and two days after the repeated application of FL the assessed clinical efficacy of the two antibiotics was roughly the same. The average efficacy of TU was 57.1±0.0%, and of florfenicol 58.5±0.0%, both the individual and mean CS in the treated calves was 10 points, due to hyperthermia, which remained the only symptom. Six days after the administration of TU and three days after the repeated application of FL both antibiotics had equal maximum efficacy (100%) in the treatment of BP. The clinical efficacy remained unchanged on day seven. The recovery was confirmed by the absence of P. multocida in nose swabs sampled on the seventh day after the initial treatment. However, in 4 calves (21.05 %) of the 19 treated Streptococcus alpha haemolyticus was isolated. Four weeks after the completion of the treatment with TU and FL the recurrence of BP caused by P. multocida was noted in 30% of calves treated with TU and 22.22% with FL. Seven days after a single administration of TU and FL, all clinical symptoms of recurrent BP and the microbiological findings were negative. Given the obtained results of the investigation on the efficacy of TU and FL in calves, TU can be recommended as the drug of first choice in the treatment of BP caused by P. multocida. Its insufficient clinical efficacy in the first three days following the application may be enhanced by simultaneous administration of NSAID and bronchodilators.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski glasnik
T1  - Uporedno ispitivanje efikasnosti tulatromicina i florfenikola u lečenju bronhopneumonije junadi
T1  - Comparative investigation on the efficacy of tulathromycin and florfenicol in the treatment of bronchopneumonia in feedlot calves
EP  - 178
IS  - 3-4
SP  - 153
VL  - 65
DO  - 10.2298/VETGL1104153J
UR  - conv_87
ER  - 
@article{
author = "Jezdimirović, Milanka and Aleksić, Nevenka and Barać-Bašević, Ana and Žutić, Milenko and Ivetić, Vojin and Radanović, Oliver and Jezdimirović, Nemanja",
year = "2011",
abstract = "Ispitivana je klinička efikasnost tulatromicina (TU) i florfenikola (FL) u lečenju bronhopneumonije (BP) prouzrokovane vrstom Pasteurella multocida, izolovanom iz briseva nosa junadi. Simptomi BP su kvantifikovani da bi se dobio klinički skor (KS) koji definiše težinu kliničke slike kod životinja. Prema korišćenoj skali maksimalna vrednost KS parametara BP iznosi 47 bodova. U našem ogledu, vrednost prosečnog KS kod obolele junadi iznosila je 23,5±0,15. Klinička efikasnost TU i FL procenjivana je svakodnevno, tokom sedam dana, na osnovu povlačenja kliničkih simptoma (smanjivanje vrednosti KS) i prisustva prouzrokovača BP u brisevima nosa 7, 28. i 35. dana od ukidanja terapije. Tulatromicin je aplikovan s.c., jednokratno u terapijskoj dozi od 2,5 mg/kg, a florfenikol s.c., dvokratno u razmaku od 48 h u terapijskoj dozi od 40 mg/kg tm. U ovom ispitivanju TU je u prva četri dana od aplikacije značajno brže od FL (P LT 0,05) dovodio do povlačenja bitnih kliničkih simptoma, iako je aplikacija FL u tom periodu ponovljena. Klinička efikasnost TU u lečenju BP junadi četvrtog dana od primene iznosila je 43,4±1,5 %, a florfenikola 27,2±1,6 %. Međutim, petog dana od aplikacije TU i FL i drugog dana od druge aplikacije FL ustanovljena je približno ista klinička efikasnost ovih antimikrobnih lekova u lečenju BP. Prosečna efikasnost TU bila je 57,1±0,0%, a FL 58,5± 0,0%. Vrednost individualnog i prosečnog KS kod junadi lečene TU i FL iznosila je 10 bodova. Od kliničkih simptoma jedino se hipertermija nije povukla. Šestog dana od aplikacije TU i FL i trećeg dana od druge aplikacije FL oba antibiotika imala su podjednaku, maksimalnu (100%) kliničku efikasnost u lečenju BP. Ista klinička efikasnost zabeležena je i sedmog dana ogleda. Izlečenje je potvrđeno odsustvom P. multocida u brisevima uzetim sedmog dana od početka lečenja. Međutim, kod 4 juneta (21,05 %) od ukupno 19 izolovan je Streptococcus alpha haemolyticus. Četiri nedelje od obustavljanja primene TU i FL uočen je recidiv BP prouzrokovane P. multocida kod 30% junadi lečene TU i 22,22% lečene FL. Posle jednokratne primene TU i FL, sedmog dana su se povukli svi klinički simptomi recidivirajuće BP, a mikrobiološki nalaz bio je negativan. Sumirajući rezultate dobijene uporednim ispitivanjem efikasnosti TU i FL u lečenju BP junadi prouzrokovane P. multocida predlažemo da se TU koristi kao lek prvog izbora u lečenju ove respiratorne infekcije. Njegova efikasnost, nedovoljna u prva tri dana od aplikacije, može da se poveća istovremenom primenom lekova iz grupe NSAIL i bronhodilatatora., The clinical efficacy of tulathromycin (TU) and florfenicol (FL) in the treatment of bronchopneumonia (BP) caused by Pasteurella multocida which was isolated from nose swabs of diseased calves has been examined. The symptoms of bronchopneumonia (BP) were quantified by means of the clinical score (CS) with a maximum of 47 points. In the current investigation the average CS in diseased calves was 23.5± 0.15. The clinical efficacy of TU and FL was assessed every day in the first week after the administration of the drugs and was based on the decrease in CS and on microbiological findings on days 7, 28 and 35 after the completion of therapy. Tulathromycin was administered s.c., in the prescribed therapeutic dose (2.5 mg/kg BW), and florfenicol s.c., twice at a 48 h interval, in its respective therapeutic dose (40 mg/kg BW). In spite of the repeated administration of FL, TU was significantly more rapid to decrease the major clinical symptoms in the first four days following the application, in comparison with FL (P LT 0.05). On the fourth day after the administration, the clinical efficacy of TU in the therapy of BP in calves was 43.4±1.5 %, and of florfenicol 27.2±1.6 %. However, five days after the application of TU and two days after the repeated application of FL the assessed clinical efficacy of the two antibiotics was roughly the same. The average efficacy of TU was 57.1±0.0%, and of florfenicol 58.5±0.0%, both the individual and mean CS in the treated calves was 10 points, due to hyperthermia, which remained the only symptom. Six days after the administration of TU and three days after the repeated application of FL both antibiotics had equal maximum efficacy (100%) in the treatment of BP. The clinical efficacy remained unchanged on day seven. The recovery was confirmed by the absence of P. multocida in nose swabs sampled on the seventh day after the initial treatment. However, in 4 calves (21.05 %) of the 19 treated Streptococcus alpha haemolyticus was isolated. Four weeks after the completion of the treatment with TU and FL the recurrence of BP caused by P. multocida was noted in 30% of calves treated with TU and 22.22% with FL. Seven days after a single administration of TU and FL, all clinical symptoms of recurrent BP and the microbiological findings were negative. Given the obtained results of the investigation on the efficacy of TU and FL in calves, TU can be recommended as the drug of first choice in the treatment of BP caused by P. multocida. Its insufficient clinical efficacy in the first three days following the application may be enhanced by simultaneous administration of NSAID and bronchodilators.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski glasnik",
title = "Uporedno ispitivanje efikasnosti tulatromicina i florfenikola u lečenju bronhopneumonije junadi, Comparative investigation on the efficacy of tulathromycin and florfenicol in the treatment of bronchopneumonia in feedlot calves",
pages = "178-153",
number = "3-4",
volume = "65",
doi = "10.2298/VETGL1104153J",
url = "conv_87"
}
Jezdimirović, M., Aleksić, N., Barać-Bašević, A., Žutić, M., Ivetić, V., Radanović, O.,& Jezdimirović, N.. (2011). Uporedno ispitivanje efikasnosti tulatromicina i florfenikola u lečenju bronhopneumonije junadi. in Veterinarski glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 65(3-4), 153-178.
https://doi.org/10.2298/VETGL1104153J
conv_87
Jezdimirović M, Aleksić N, Barać-Bašević A, Žutić M, Ivetić V, Radanović O, Jezdimirović N. Uporedno ispitivanje efikasnosti tulatromicina i florfenikola u lečenju bronhopneumonije junadi. in Veterinarski glasnik. 2011;65(3-4):153-178.
doi:10.2298/VETGL1104153J
conv_87 .
Jezdimirović, Milanka, Aleksić, Nevenka, Barać-Bašević, Ana, Žutić, Milenko, Ivetić, Vojin, Radanović, Oliver, Jezdimirović, Nemanja, "Uporedno ispitivanje efikasnosti tulatromicina i florfenikola u lečenju bronhopneumonije junadi" in Veterinarski glasnik, 65, no. 3-4 (2011):153-178,
https://doi.org/10.2298/VETGL1104153J .,
conv_87 .

Detection rates of the swine torque teno viruses (TTVs), porcine circovirus type 2 (PCV2) and hepatitis E virus (HEV) in the livers of pigs with hepatitis

Savić, Božidar; Milićević, Vesna; Bojkovski, Jovan; Kureljušić, Branislav; Ivetić, Vojin; Pavlović, Ivan

(Springer, Dordrecht, 2010)

TY  - JOUR
AU  - Savić, Božidar
AU  - Milićević, Vesna
AU  - Bojkovski, Jovan
AU  - Kureljušić, Branislav
AU  - Ivetić, Vojin
AU  - Pavlović, Ivan
PY  - 2010
UR  - https://reponivs.nivs.rs/handle/123456789/158
AB  - Porcine circovirus type 2 (PCV2) and hepatitis E virus (HEV) are the most recently recognized causes of infectious hepatitis of pigs and may or may not act independently in the development of the disease. Recently it has been suggested that swine torque teno viruses (TTVs), in co-infections with some swine viral pathogens, may potentiate the severity of disease. In order to search for virological cofactors associated with infectious hepatitis in pigs, we investigated the liver tissues, to determine the presence of TTVs, PCV2 and HEV of naturally infected pigs and analysed the prevalence of both genogroups of the TTVs in the hepatitis lesions. Histopathological techniques, nested-polymerase chain reactions (nPCRs), polymerase chain reaction (PCR) and one-step reverse transcriptase polymerase chain reaction (RT-PCR) were applied to detect hepatitis lesions, TTVs genogroups 1 and 2, PCV2 and HEV infection. Of the livers examined 58% (29/50) had mild to moderate hepatitis and 74% (37/50), 56% (28/50) and 26% (13/50) samples were nPCR, PCR and RT-PCR positive for TTVs PCV2 and HEV respectively. TTVs were detected in 84% (16/19) of the samples which were determined to be of mild severity while present in almost all (90% or 9/10) samples identified as having moderate hepatitis lesions. Additionally, the livers of 12 out of 21 (57%) pigs without the hepatitis lesions were positive for TTVs. These results demonstrate an association between TTVs and infectious hepatitis of pigs in concomitant infections with PCV2 and/or HEV and indicated that TTVs may play a role as a cofactor in the pathogenesis of disease.
PB  - Springer, Dordrecht
T2  - Veterinary Research Communications
T1  - Detection rates of the swine torque teno viruses (TTVs), porcine circovirus type 2 (PCV2) and hepatitis E virus (HEV) in the livers of pigs with hepatitis
EP  - 648
IS  - 7
SP  - 641
VL  - 34
DO  - 10.1007/s11259-010-9432-z
UR  - conv_375
ER  - 
@article{
author = "Savić, Božidar and Milićević, Vesna and Bojkovski, Jovan and Kureljušić, Branislav and Ivetić, Vojin and Pavlović, Ivan",
year = "2010",
abstract = "Porcine circovirus type 2 (PCV2) and hepatitis E virus (HEV) are the most recently recognized causes of infectious hepatitis of pigs and may or may not act independently in the development of the disease. Recently it has been suggested that swine torque teno viruses (TTVs), in co-infections with some swine viral pathogens, may potentiate the severity of disease. In order to search for virological cofactors associated with infectious hepatitis in pigs, we investigated the liver tissues, to determine the presence of TTVs, PCV2 and HEV of naturally infected pigs and analysed the prevalence of both genogroups of the TTVs in the hepatitis lesions. Histopathological techniques, nested-polymerase chain reactions (nPCRs), polymerase chain reaction (PCR) and one-step reverse transcriptase polymerase chain reaction (RT-PCR) were applied to detect hepatitis lesions, TTVs genogroups 1 and 2, PCV2 and HEV infection. Of the livers examined 58% (29/50) had mild to moderate hepatitis and 74% (37/50), 56% (28/50) and 26% (13/50) samples were nPCR, PCR and RT-PCR positive for TTVs PCV2 and HEV respectively. TTVs were detected in 84% (16/19) of the samples which were determined to be of mild severity while present in almost all (90% or 9/10) samples identified as having moderate hepatitis lesions. Additionally, the livers of 12 out of 21 (57%) pigs without the hepatitis lesions were positive for TTVs. These results demonstrate an association between TTVs and infectious hepatitis of pigs in concomitant infections with PCV2 and/or HEV and indicated that TTVs may play a role as a cofactor in the pathogenesis of disease.",
publisher = "Springer, Dordrecht",
journal = "Veterinary Research Communications",
title = "Detection rates of the swine torque teno viruses (TTVs), porcine circovirus type 2 (PCV2) and hepatitis E virus (HEV) in the livers of pigs with hepatitis",
pages = "648-641",
number = "7",
volume = "34",
doi = "10.1007/s11259-010-9432-z",
url = "conv_375"
}
Savić, B., Milićević, V., Bojkovski, J., Kureljušić, B., Ivetić, V.,& Pavlović, I.. (2010). Detection rates of the swine torque teno viruses (TTVs), porcine circovirus type 2 (PCV2) and hepatitis E virus (HEV) in the livers of pigs with hepatitis. in Veterinary Research Communications
Springer, Dordrecht., 34(7), 641-648.
https://doi.org/10.1007/s11259-010-9432-z
conv_375
Savić B, Milićević V, Bojkovski J, Kureljušić B, Ivetić V, Pavlović I. Detection rates of the swine torque teno viruses (TTVs), porcine circovirus type 2 (PCV2) and hepatitis E virus (HEV) in the livers of pigs with hepatitis. in Veterinary Research Communications. 2010;34(7):641-648.
doi:10.1007/s11259-010-9432-z
conv_375 .
Savić, Božidar, Milićević, Vesna, Bojkovski, Jovan, Kureljušić, Branislav, Ivetić, Vojin, Pavlović, Ivan, "Detection rates of the swine torque teno viruses (TTVs), porcine circovirus type 2 (PCV2) and hepatitis E virus (HEV) in the livers of pigs with hepatitis" in Veterinary Research Communications, 34, no. 7 (2010):641-648,
https://doi.org/10.1007/s11259-010-9432-z .,
conv_375 .
26
25
30

Genetic diversity of mycoplasma hyopneumoniae isolates from conventional farrow-to-finish pig farms in Serbia

Savić, Božidar; Ivetić, Vojin; Milićević, Vesna; Pavlović, Ivan; Žutić, Milenko; Gagrcin, Mladen

(Akademiai Kiado Zrt, Budapest, 2010)

TY  - JOUR
AU  - Savić, Božidar
AU  - Ivetić, Vojin
AU  - Milićević, Vesna
AU  - Pavlović, Ivan
AU  - Žutić, Milenko
AU  - Gagrcin, Mladen
PY  - 2010
UR  - https://reponivs.nivs.rs/handle/123456789/150
AB  - Mycoplasma hyopneumoniae is a primary agent associated with mycoplasma pneumonia and the porcine respiratory disease complex (PRDC). Various reports have indicated that different strains of M. hyopneumoniae are circulating in the swine population. Lysates from lung swabs from naturally infected pigs of different ages were tested according to a new variable number of tandem repeats (VNTR) genetic typing method based on the polyserine repeat motif of the P146 lipoproteoadhesin, which can be applied directly on clinical material without isolation of M. hyopneumoniae. The aim was to determine the diversity of M. hyopneumoniae isolates from conventional farrow-to-finish pig farms located in different geographical areas of Serbia. PCR amplification was carried out using M. hyopneumoniae-specific designed, conserved primers (p146MH - L and p146MH - R) flanking the region encoding the repeat motif, followed by sequencing and cluster analysis. Five groups of M. hyopneumoniae with thirteen to twenty-four serine repeats were observed. Analysis of three samples from each farm indicated that the specific isolate is ubiquitous in pigs of different ages. Furthermore, seven clusters were observed within 27 tested samples. The results indicated a considerable diversity among M. hyopneumoniae field isolates in the swine population from conventional farrow-to-finish farms in Serbia and suggest close genetic relatedness of the corresponding isolates.
PB  - Akademiai Kiado Zrt, Budapest
T2  - Acta Veterinaria Hungarica
T1  - Genetic diversity of mycoplasma hyopneumoniae isolates from conventional farrow-to-finish pig farms in Serbia
EP  - 308
IS  - 3
SP  - 297
VL  - 58
DO  - 10.1556/AVet.58.2010.3.3
UR  - conv_374
ER  - 
@article{
author = "Savić, Božidar and Ivetić, Vojin and Milićević, Vesna and Pavlović, Ivan and Žutić, Milenko and Gagrcin, Mladen",
year = "2010",
abstract = "Mycoplasma hyopneumoniae is a primary agent associated with mycoplasma pneumonia and the porcine respiratory disease complex (PRDC). Various reports have indicated that different strains of M. hyopneumoniae are circulating in the swine population. Lysates from lung swabs from naturally infected pigs of different ages were tested according to a new variable number of tandem repeats (VNTR) genetic typing method based on the polyserine repeat motif of the P146 lipoproteoadhesin, which can be applied directly on clinical material without isolation of M. hyopneumoniae. The aim was to determine the diversity of M. hyopneumoniae isolates from conventional farrow-to-finish pig farms located in different geographical areas of Serbia. PCR amplification was carried out using M. hyopneumoniae-specific designed, conserved primers (p146MH - L and p146MH - R) flanking the region encoding the repeat motif, followed by sequencing and cluster analysis. Five groups of M. hyopneumoniae with thirteen to twenty-four serine repeats were observed. Analysis of three samples from each farm indicated that the specific isolate is ubiquitous in pigs of different ages. Furthermore, seven clusters were observed within 27 tested samples. The results indicated a considerable diversity among M. hyopneumoniae field isolates in the swine population from conventional farrow-to-finish farms in Serbia and suggest close genetic relatedness of the corresponding isolates.",
publisher = "Akademiai Kiado Zrt, Budapest",
journal = "Acta Veterinaria Hungarica",
title = "Genetic diversity of mycoplasma hyopneumoniae isolates from conventional farrow-to-finish pig farms in Serbia",
pages = "308-297",
number = "3",
volume = "58",
doi = "10.1556/AVet.58.2010.3.3",
url = "conv_374"
}
Savić, B., Ivetić, V., Milićević, V., Pavlović, I., Žutić, M.,& Gagrcin, M.. (2010). Genetic diversity of mycoplasma hyopneumoniae isolates from conventional farrow-to-finish pig farms in Serbia. in Acta Veterinaria Hungarica
Akademiai Kiado Zrt, Budapest., 58(3), 297-308.
https://doi.org/10.1556/AVet.58.2010.3.3
conv_374
Savić B, Ivetić V, Milićević V, Pavlović I, Žutić M, Gagrcin M. Genetic diversity of mycoplasma hyopneumoniae isolates from conventional farrow-to-finish pig farms in Serbia. in Acta Veterinaria Hungarica. 2010;58(3):297-308.
doi:10.1556/AVet.58.2010.3.3
conv_374 .
Savić, Božidar, Ivetić, Vojin, Milićević, Vesna, Pavlović, Ivan, Žutić, Milenko, Gagrcin, Mladen, "Genetic diversity of mycoplasma hyopneumoniae isolates from conventional farrow-to-finish pig farms in Serbia" in Acta Veterinaria Hungarica, 58, no. 3 (2010):297-308,
https://doi.org/10.1556/AVet.58.2010.3.3 .,
conv_374 .
8
7
11

Immunohistochemical study of the inflammatory infiltrate associated with swine hepatitis e virus infection

Kureljušić, Branislav; Ivetić, Vojin; Savić, Božidar; Kukolj, Vladimir; Milićević, Vesna; Aleksić-Kovačević, Sanja

(Elsevier Sci Ltd, Oxford, 2010)

TY  - CONF
AU  - Kureljušić, Branislav
AU  - Ivetić, Vojin
AU  - Savić, Božidar
AU  - Kukolj, Vladimir
AU  - Milićević, Vesna
AU  - Aleksić-Kovačević, Sanja
PY  - 2010
UR  - https://reponivs.nivs.rs/handle/123456789/146
PB  - Elsevier Sci Ltd, Oxford
C3  - Journal of Comparative Pathology
T1  - Immunohistochemical study of the inflammatory infiltrate associated with swine hepatitis e virus infection
EP  - 322
IS  - 4
SP  - 322
VL  - 143
DO  - 10.1016/j.jcpa.2010.09.037
UR  - conv_383
ER  - 
@conference{
author = "Kureljušić, Branislav and Ivetić, Vojin and Savić, Božidar and Kukolj, Vladimir and Milićević, Vesna and Aleksić-Kovačević, Sanja",
year = "2010",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Journal of Comparative Pathology",
title = "Immunohistochemical study of the inflammatory infiltrate associated with swine hepatitis e virus infection",
pages = "322-322",
number = "4",
volume = "143",
doi = "10.1016/j.jcpa.2010.09.037",
url = "conv_383"
}
Kureljušić, B., Ivetić, V., Savić, B., Kukolj, V., Milićević, V.,& Aleksić-Kovačević, S.. (2010). Immunohistochemical study of the inflammatory infiltrate associated with swine hepatitis e virus infection. in Journal of Comparative Pathology
Elsevier Sci Ltd, Oxford., 143(4), 322-322.
https://doi.org/10.1016/j.jcpa.2010.09.037
conv_383
Kureljušić B, Ivetić V, Savić B, Kukolj V, Milićević V, Aleksić-Kovačević S. Immunohistochemical study of the inflammatory infiltrate associated with swine hepatitis e virus infection. in Journal of Comparative Pathology. 2010;143(4):322-322.
doi:10.1016/j.jcpa.2010.09.037
conv_383 .
Kureljušić, Branislav, Ivetić, Vojin, Savić, Božidar, Kukolj, Vladimir, Milićević, Vesna, Aleksić-Kovačević, Sanja, "Immunohistochemical study of the inflammatory infiltrate associated with swine hepatitis e virus infection" in Journal of Comparative Pathology, 143, no. 4 (2010):322-322,
https://doi.org/10.1016/j.jcpa.2010.09.037 .,
conv_383 .

PCR - dijagnostika infekcije kod svinja koju izaziva Mycoplasma suis

Potkonjak, Aleksandar; Lako, Branislav; Milićević, Vesna; Savić, Božidar; Ivetić, Vojin; Jakić-Dimić, Dobrila; Stevančević, Ognjen; Toholj, Bojan

(Beograd : Fakultet veterinarske medicine, 2009)

TY  - JOUR
AU  - Potkonjak, Aleksandar
AU  - Lako, Branislav
AU  - Milićević, Vesna
AU  - Savić, Božidar
AU  - Ivetić, Vojin
AU  - Jakić-Dimić, Dobrila
AU  - Stevančević, Ognjen
AU  - Toholj, Bojan
PY  - 2009
UR  - https://reponivs.nivs.rs/handle/123456789/141
AB  - Patogena hemoplazma, nazvana Mycoplasma suis (prethodni naziv Eperythrozoon suis) je uzročnik eperitrozoonoze svinja ili ikteroanemije svinja. Uzročnik može inficirati i ljude. Oboljenje je veoma rasprostranjeno u svetu. Najčešća je latentna infekcija svinja izazvana M. suis. Nakon delovanja faktora stresa, oboljenje se klinički manifestuje. Akutni tok se odlikuje pojavom febrilnog stanja i ikteroanemije. Dijagnoza oboljenja se, uobičajeno, postavlja na osnovu epizootiološke ankete, kliničkog pregleda i mikroskopskog pregleda razmaza krvi svinja obojenog, najčešće, po Gimzi. Razvijene su savremene metode molekularne biologije poput PCR, koje su osetljivije i specifičnije u postavljanju dijagnoze infekcije svinja izazvanih M. suis. Primenom PCR testa, ovim ispitivanjem je utvrđeno prisustvo M. suis na farmama svinja u Republici Srbiji.
AB  - The presence of two types of haemoplasm can be established in the swine population. Pathogenic haemoplasm, named Mycoplasma suis (previously called Eperythrozoon suis) is the cause of swine eperythrozoonosis or swine ichtheroanaemia. The cause of this disease can also infect humans. The disease has spread all over the world. The most frequent form is latent infection of swine caused by M. suis. The disease is clinically manifest following action by the stress factor. The acute course of the disease is characterized by the occurrence of a febrile condition and ichtheroanaemia. The disease is usually diagnosed based on an epizootiological poll, a clinical examination, and a microscopic examination of a blood smear stained most often according to Giemsa. Contemporary methods of molecular biology have been developed, such as PCR, which are more sensitive and specific in making a diagnosis of swine infection caused by M. suis. In these investigations, the presence of M. suis on pig farms in the Republic of Serbia has been determined using the PCR test. .
PB  - Beograd : Fakultet veterinarske medicine
T2  - Veterinarski glasnik
T1  - PCR - dijagnostika infekcije kod svinja koju izaziva Mycoplasma suis
T1  - Molecular diagnostics of swine infection caused by Mycoplasma suis
EP  - 358
IS  - 5-6
SP  - 353
VL  - 63
DO  - 10.2298/VETGL0906353P
UR  - conv_79
ER  - 
@article{
author = "Potkonjak, Aleksandar and Lako, Branislav and Milićević, Vesna and Savić, Božidar and Ivetić, Vojin and Jakić-Dimić, Dobrila and Stevančević, Ognjen and Toholj, Bojan",
year = "2009",
abstract = "Patogena hemoplazma, nazvana Mycoplasma suis (prethodni naziv Eperythrozoon suis) je uzročnik eperitrozoonoze svinja ili ikteroanemije svinja. Uzročnik može inficirati i ljude. Oboljenje je veoma rasprostranjeno u svetu. Najčešća je latentna infekcija svinja izazvana M. suis. Nakon delovanja faktora stresa, oboljenje se klinički manifestuje. Akutni tok se odlikuje pojavom febrilnog stanja i ikteroanemije. Dijagnoza oboljenja se, uobičajeno, postavlja na osnovu epizootiološke ankete, kliničkog pregleda i mikroskopskog pregleda razmaza krvi svinja obojenog, najčešće, po Gimzi. Razvijene su savremene metode molekularne biologije poput PCR, koje su osetljivije i specifičnije u postavljanju dijagnoze infekcije svinja izazvanih M. suis. Primenom PCR testa, ovim ispitivanjem je utvrđeno prisustvo M. suis na farmama svinja u Republici Srbiji., The presence of two types of haemoplasm can be established in the swine population. Pathogenic haemoplasm, named Mycoplasma suis (previously called Eperythrozoon suis) is the cause of swine eperythrozoonosis or swine ichtheroanaemia. The cause of this disease can also infect humans. The disease has spread all over the world. The most frequent form is latent infection of swine caused by M. suis. The disease is clinically manifest following action by the stress factor. The acute course of the disease is characterized by the occurrence of a febrile condition and ichtheroanaemia. The disease is usually diagnosed based on an epizootiological poll, a clinical examination, and a microscopic examination of a blood smear stained most often according to Giemsa. Contemporary methods of molecular biology have been developed, such as PCR, which are more sensitive and specific in making a diagnosis of swine infection caused by M. suis. In these investigations, the presence of M. suis on pig farms in the Republic of Serbia has been determined using the PCR test. .",
publisher = "Beograd : Fakultet veterinarske medicine",
journal = "Veterinarski glasnik",
title = "PCR - dijagnostika infekcije kod svinja koju izaziva Mycoplasma suis, Molecular diagnostics of swine infection caused by Mycoplasma suis",
pages = "358-353",
number = "5-6",
volume = "63",
doi = "10.2298/VETGL0906353P",
url = "conv_79"
}
Potkonjak, A., Lako, B., Milićević, V., Savić, B., Ivetić, V., Jakić-Dimić, D., Stevančević, O.,& Toholj, B.. (2009). PCR - dijagnostika infekcije kod svinja koju izaziva Mycoplasma suis. in Veterinarski glasnik
Beograd : Fakultet veterinarske medicine., 63(5-6), 353-358.
https://doi.org/10.2298/VETGL0906353P
conv_79
Potkonjak A, Lako B, Milićević V, Savić B, Ivetić V, Jakić-Dimić D, Stevančević O, Toholj B. PCR - dijagnostika infekcije kod svinja koju izaziva Mycoplasma suis. in Veterinarski glasnik. 2009;63(5-6):353-358.
doi:10.2298/VETGL0906353P
conv_79 .
Potkonjak, Aleksandar, Lako, Branislav, Milićević, Vesna, Savić, Božidar, Ivetić, Vojin, Jakić-Dimić, Dobrila, Stevančević, Ognjen, Toholj, Bojan, "PCR - dijagnostika infekcije kod svinja koju izaziva Mycoplasma suis" in Veterinarski glasnik, 63, no. 5-6 (2009):353-358,
https://doi.org/10.2298/VETGL0906353P .,
conv_79 .
1

Želudačno-crevna strongilidoza koza i ovaca na području Beograda

Pavlović, Ivan; Ivetić, Vojin; Savić, Božidar; Radanović, Oliver; Žutić, Milenko; Ivanović, Snežana

(Beograd : Institut PKB Agroekonomik, 2009)

TY  - JOUR
AU  - Pavlović, Ivan
AU  - Ivetić, Vojin
AU  - Savić, Božidar
AU  - Radanović, Oliver
AU  - Žutić, Milenko
AU  - Ivanović, Snežana
PY  - 2009
UR  - https://reponivs.nivs.rs/handle/123456789/140
AB  - U cilju kontrole parazitskih infekcija koza i ovaca u pojedinim delovima Srbije otpočeta su istraživanja njihove parazitofaune. Na području Beograda koprološki je pregledano 531 stado koza i ovaca iz 23 sela sa područja opština Mladenovac, Lazarevac, Obrenovac, Grocka, Zemun, Surčin, Palilula, Vozdovac i Zvezdara. Utvrđeno je prisustvo sledećih želudačno crevnih stronglida: Ostertagia circumcincta (95,23%), O. trifurcata (91,53%), O.ostertagi (23,33%), Trchostrongylus axei (100%), T. colubriformis (89,57%), T. vitrinus (81,21%), T. capricola (62,85%), Nematodirus spathiger (100%), N. filicolis (43,31%), N. abnormalis (11,64%), Hameonchus contortus (88,95%), Marshallagia marshalli (23,77%), Skrjabinema ovis (23,33%), S. caprae (13,28%), Chabertia ovina (64,14%), Oesophagostomum venulosum (28,39%), i Bunostomim trigonocephalum (12,28%).
AB  - In aim of control parasitic infection of goat and sheep we started to systematic parasitological examination of it. At Belgrade area we examined 531 flocks of goats and sheep originated from 23 vilages from city districts Mladenovac, Lazarevac, Obrenovac, Grocka, Zemun, Surčin, Palilula, Vozdovac and Zvezdara. We found nex gastrointestinal strongilidae: Ostertagia circumcincta (95,23%), O. trifurcata (91,53%), O. ostertagi (23,33%), Trchostrongylus axei (100%), T. colubriformis (89,57%), T. vitrinus (81,21%), T. capricola (62,85%),Nematodirus spathiger (100%), N. filicolis (43,31%), N. abnormalis (11,64%), Hameonchus contortus (88,95%), Marshallagia marshalli (23,77%), Skrjabinema ovis (23,33%), S. caprae (13,28%), Chabertia ovina (64,14%), Oesophagostomum venulosum (28,39%), and Bunostomim trigonocephalum (12,28%).
PB  - Beograd : Institut PKB Agroekonomik
T2  - Zbornik naučnih radova Instituta PKB Agroekonomik
T1  - Želudačno-crevna strongilidoza koza i ovaca na području Beograda
T1  - Gastrointestinal strongilides of goats and sheep in Belgrade area
EP  - 127
IS  - 3-4
SP  - 123
VL  - 15
UR  - conv_155
ER  - 
@article{
author = "Pavlović, Ivan and Ivetić, Vojin and Savić, Božidar and Radanović, Oliver and Žutić, Milenko and Ivanović, Snežana",
year = "2009",
abstract = "U cilju kontrole parazitskih infekcija koza i ovaca u pojedinim delovima Srbije otpočeta su istraživanja njihove parazitofaune. Na području Beograda koprološki je pregledano 531 stado koza i ovaca iz 23 sela sa područja opština Mladenovac, Lazarevac, Obrenovac, Grocka, Zemun, Surčin, Palilula, Vozdovac i Zvezdara. Utvrđeno je prisustvo sledećih želudačno crevnih stronglida: Ostertagia circumcincta (95,23%), O. trifurcata (91,53%), O.ostertagi (23,33%), Trchostrongylus axei (100%), T. colubriformis (89,57%), T. vitrinus (81,21%), T. capricola (62,85%), Nematodirus spathiger (100%), N. filicolis (43,31%), N. abnormalis (11,64%), Hameonchus contortus (88,95%), Marshallagia marshalli (23,77%), Skrjabinema ovis (23,33%), S. caprae (13,28%), Chabertia ovina (64,14%), Oesophagostomum venulosum (28,39%), i Bunostomim trigonocephalum (12,28%)., In aim of control parasitic infection of goat and sheep we started to systematic parasitological examination of it. At Belgrade area we examined 531 flocks of goats and sheep originated from 23 vilages from city districts Mladenovac, Lazarevac, Obrenovac, Grocka, Zemun, Surčin, Palilula, Vozdovac and Zvezdara. We found nex gastrointestinal strongilidae: Ostertagia circumcincta (95,23%), O. trifurcata (91,53%), O. ostertagi (23,33%), Trchostrongylus axei (100%), T. colubriformis (89,57%), T. vitrinus (81,21%), T. capricola (62,85%),Nematodirus spathiger (100%), N. filicolis (43,31%), N. abnormalis (11,64%), Hameonchus contortus (88,95%), Marshallagia marshalli (23,77%), Skrjabinema ovis (23,33%), S. caprae (13,28%), Chabertia ovina (64,14%), Oesophagostomum venulosum (28,39%), and Bunostomim trigonocephalum (12,28%).",
publisher = "Beograd : Institut PKB Agroekonomik",
journal = "Zbornik naučnih radova Instituta PKB Agroekonomik",
title = "Želudačno-crevna strongilidoza koza i ovaca na području Beograda, Gastrointestinal strongilides of goats and sheep in Belgrade area",
pages = "127-123",
number = "3-4",
volume = "15",
url = "conv_155"
}
Pavlović, I., Ivetić, V., Savić, B., Radanović, O., Žutić, M.,& Ivanović, S.. (2009). Želudačno-crevna strongilidoza koza i ovaca na području Beograda. in Zbornik naučnih radova Instituta PKB Agroekonomik
Beograd : Institut PKB Agroekonomik., 15(3-4), 123-127.
conv_155
Pavlović I, Ivetić V, Savić B, Radanović O, Žutić M, Ivanović S. Želudačno-crevna strongilidoza koza i ovaca na području Beograda. in Zbornik naučnih radova Instituta PKB Agroekonomik. 2009;15(3-4):123-127.
conv_155 .
Pavlović, Ivan, Ivetić, Vojin, Savić, Božidar, Radanović, Oliver, Žutić, Milenko, Ivanović, Snežana, "Želudačno-crevna strongilidoza koza i ovaca na području Beograda" in Zbornik naučnih radova Instituta PKB Agroekonomik, 15, no. 3-4 (2009):123-127,
conv_155 .