West Nile virus in the Republic of Serbia—Diagnostic performance of five serological tests in dog and horse sera
Authors
Vasić, Ana![](/themes/Miragereponivs/images/orcid.png)
Raileanu, Cristian
![](/themes/Miragereponivs/images/orcid.png)
Körsten, Christin
![](/themes/Miragereponivs/images/orcid.png)
Vojinović, Dragica
![](/themes/Miragereponivs/images/orcid.png)
Manić, Marija
![](/themes/Miragereponivs/images/orcid.png)
Urošević, Aleksandar
Nikolić, Nataša
Dulović, Olga
Tews, Birke Andrea
![](/themes/Miragereponivs/images/orcid.png)
Petrović, Tamaš
![](/themes/Miragereponivs/images/orcid.png)
Silaghi, Cornelia
Valcić, Miroslav
Gligić, Ana
Article (Published version)
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West Nile virus (WNV) is a zoonotic mosquito-borne virus classified as family Flaviviridae and genus Flavivirus. The first WNV outbreak in humans in the Republic of Serbia was recorded in 2012. Equids and dogs can show clinical symptoms after WNV infection and are often used as sentinels. This study aimed to (i) give insight into seropositivity for WNV in clinically healthy dog and horse sera in different regions of Serbia and (ii) compare diagnostic value of ‘in-house’ and commercially available indirect immunofluorescence (IFA) and enzyme-linked immunoassay (ELISA) tests to ‘gold standard’ virus neutralization test (VNT). Due to cross-reactivity, sera were tested for Usutu virus and tick-borne encephalitis virus in VNT based on the epidemiological data of field presence. Blood sera of dogs (n = 184) and horses (n = 232) were collected from 2011 to 2013. The seropositivity was confirmed by VNT in 36.9 % tested dog sera and 34.9% tested horse sera with highest positivity in regions nea...r two big rivers, while in four dog and seven horse sera, positivity resulted from Usutu virus infection. Comparative results of diagnostic tests in dogs ranged from 18.7 % seropositivity by ‘in-house’ ELISA to 31.9% by commercially available ELISA. In horses, seropositivity ranged from 36.2% by ‘in-house’ IFA to 32.5% by commercially available IFA and from 26.3% by ‘in-house’ IgG ELISA to 20.9% by commercially available ELISA. There were no statistically significant differences according to the McNemar test between ‘in-house’ and commercially available IFA and ELISA test in horse sera, while the same was not true for two ELISAs used in dog sera (χ2 = 8.647, p = .003). Established seropositivity in dogs and horses was in accordance with the epidemiological situation and WNV spread in the Republic of Serbia and proven Usutu virus co-circulation. ‘In-house’ tests remain a valuable tool in early diagnostics of WNV.
Keywords:
dog / horses / Serbia / serology / West Nile virusSource:
Transboundary and Emerging Diseases, 2022, 69, 5, 2506-2515Publisher:
- Hoboken : Wiley
Funding / projects:
- Ecological and viral investigation on the presents of emerging zoonoses in national parks in republic of Serbia (RS-MESTD-Technological Development (TD or TR)-37015)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200015 (University of Belgrade, Institute for Medical Research) (RS-MESTD-inst-2020-200015)
DOI: 10.1111/tbed.14593
ISSN: 1865-1682; 1865-1674
PubMed: 35538046
WoS: 000799501800001
Scopus: 2-s2.0-85130430447
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Institution/Community
Naučni institut za veterinarstvo SrbijeTY - JOUR AU - Vasić, Ana AU - Raileanu, Cristian AU - Körsten, Christin AU - Vojinović, Dragica AU - Manić, Marija AU - Urošević, Aleksandar AU - Nikolić, Nataša AU - Dulović, Olga AU - Tews, Birke Andrea AU - Petrović, Tamaš AU - Silaghi, Cornelia AU - Valcić, Miroslav AU - Gligić, Ana PY - 2022 UR - https://reponivs.nivs.rs/handle/123456789/519 AB - West Nile virus (WNV) is a zoonotic mosquito-borne virus classified as family Flaviviridae and genus Flavivirus. The first WNV outbreak in humans in the Republic of Serbia was recorded in 2012. Equids and dogs can show clinical symptoms after WNV infection and are often used as sentinels. This study aimed to (i) give insight into seropositivity for WNV in clinically healthy dog and horse sera in different regions of Serbia and (ii) compare diagnostic value of ‘in-house’ and commercially available indirect immunofluorescence (IFA) and enzyme-linked immunoassay (ELISA) tests to ‘gold standard’ virus neutralization test (VNT). Due to cross-reactivity, sera were tested for Usutu virus and tick-borne encephalitis virus in VNT based on the epidemiological data of field presence. Blood sera of dogs (n = 184) and horses (n = 232) were collected from 2011 to 2013. The seropositivity was confirmed by VNT in 36.9 % tested dog sera and 34.9% tested horse sera with highest positivity in regions near two big rivers, while in four dog and seven horse sera, positivity resulted from Usutu virus infection. Comparative results of diagnostic tests in dogs ranged from 18.7 % seropositivity by ‘in-house’ ELISA to 31.9% by commercially available ELISA. In horses, seropositivity ranged from 36.2% by ‘in-house’ IFA to 32.5% by commercially available IFA and from 26.3% by ‘in-house’ IgG ELISA to 20.9% by commercially available ELISA. There were no statistically significant differences according to the McNemar test between ‘in-house’ and commercially available IFA and ELISA test in horse sera, while the same was not true for two ELISAs used in dog sera (χ2 = 8.647, p = .003). Established seropositivity in dogs and horses was in accordance with the epidemiological situation and WNV spread in the Republic of Serbia and proven Usutu virus co-circulation. ‘In-house’ tests remain a valuable tool in early diagnostics of WNV. PB - Hoboken : Wiley T2 - Transboundary and Emerging Diseases T1 - West Nile virus in the Republic of Serbia—Diagnostic performance of five serological tests in dog and horse sera EP - 2515 IS - 5 SP - 2506 VL - 69 DO - 10.1111/tbed.14593 ER -
@article{ author = "Vasić, Ana and Raileanu, Cristian and Körsten, Christin and Vojinović, Dragica and Manić, Marija and Urošević, Aleksandar and Nikolić, Nataša and Dulović, Olga and Tews, Birke Andrea and Petrović, Tamaš and Silaghi, Cornelia and Valcić, Miroslav and Gligić, Ana", year = "2022", abstract = "West Nile virus (WNV) is a zoonotic mosquito-borne virus classified as family Flaviviridae and genus Flavivirus. The first WNV outbreak in humans in the Republic of Serbia was recorded in 2012. Equids and dogs can show clinical symptoms after WNV infection and are often used as sentinels. This study aimed to (i) give insight into seropositivity for WNV in clinically healthy dog and horse sera in different regions of Serbia and (ii) compare diagnostic value of ‘in-house’ and commercially available indirect immunofluorescence (IFA) and enzyme-linked immunoassay (ELISA) tests to ‘gold standard’ virus neutralization test (VNT). Due to cross-reactivity, sera were tested for Usutu virus and tick-borne encephalitis virus in VNT based on the epidemiological data of field presence. Blood sera of dogs (n = 184) and horses (n = 232) were collected from 2011 to 2013. The seropositivity was confirmed by VNT in 36.9 % tested dog sera and 34.9% tested horse sera with highest positivity in regions near two big rivers, while in four dog and seven horse sera, positivity resulted from Usutu virus infection. Comparative results of diagnostic tests in dogs ranged from 18.7 % seropositivity by ‘in-house’ ELISA to 31.9% by commercially available ELISA. In horses, seropositivity ranged from 36.2% by ‘in-house’ IFA to 32.5% by commercially available IFA and from 26.3% by ‘in-house’ IgG ELISA to 20.9% by commercially available ELISA. There were no statistically significant differences according to the McNemar test between ‘in-house’ and commercially available IFA and ELISA test in horse sera, while the same was not true for two ELISAs used in dog sera (χ2 = 8.647, p = .003). Established seropositivity in dogs and horses was in accordance with the epidemiological situation and WNV spread in the Republic of Serbia and proven Usutu virus co-circulation. ‘In-house’ tests remain a valuable tool in early diagnostics of WNV.", publisher = "Hoboken : Wiley", journal = "Transboundary and Emerging Diseases", title = "West Nile virus in the Republic of Serbia—Diagnostic performance of five serological tests in dog and horse sera", pages = "2515-2506", number = "5", volume = "69", doi = "10.1111/tbed.14593" }
Vasić, A., Raileanu, C., Körsten, C., Vojinović, D., Manić, M., Urošević, A., Nikolić, N., Dulović, O., Tews, B. A., Petrović, T., Silaghi, C., Valcić, M.,& Gligić, A.. (2022). West Nile virus in the Republic of Serbia—Diagnostic performance of five serological tests in dog and horse sera. in Transboundary and Emerging Diseases Hoboken : Wiley., 69(5), 2506-2515. https://doi.org/10.1111/tbed.14593
Vasić A, Raileanu C, Körsten C, Vojinović D, Manić M, Urošević A, Nikolić N, Dulović O, Tews BA, Petrović T, Silaghi C, Valcić M, Gligić A. West Nile virus in the Republic of Serbia—Diagnostic performance of five serological tests in dog and horse sera. in Transboundary and Emerging Diseases. 2022;69(5):2506-2515. doi:10.1111/tbed.14593 .
Vasić, Ana, Raileanu, Cristian, Körsten, Christin, Vojinović, Dragica, Manić, Marija, Urošević, Aleksandar, Nikolić, Nataša, Dulović, Olga, Tews, Birke Andrea, Petrović, Tamaš, Silaghi, Cornelia, Valcić, Miroslav, Gligić, Ana, "West Nile virus in the Republic of Serbia—Diagnostic performance of five serological tests in dog and horse sera" in Transboundary and Emerging Diseases, 69, no. 5 (2022):2506-2515, https://doi.org/10.1111/tbed.14593 . .