Evaluation of West Nile Virus Diagnostic Capacities in Veterinary Laboratories of the Mediterranean and Black Sea Regions
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2020
Authors
Perez-Ramirez, Elisa![](/themes/Miragereponivs/images/orcid.png)
Cano-Gomez, Cristina
![](/themes/Miragereponivs/images/orcid.png)
Llorente, Francisco
![](/themes/Miragereponivs/images/orcid.png)
Vodica, Ani
Veljović, Ljubiša
![](/themes/Miragereponivs/images/orcid.png)
Toklikishvilli, Natela
Sherifi, Kurtesh
![](/themes/Miragereponivs/images/orcid.png)
Sghaier, Soufien
![](/themes/Miragereponivs/images/orcid.png)
Omani, Amel
Kustura, Aida
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Krstevski, Kiril
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Karayel-Hacioglu, Ilke
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Hagag, Naglaa Mohamed
El Hage, Jeanne
Davdyan, Hasmik
Bintarif, Mohd Saddam
Adžić, Bojan
Abouchoaib, Nabil
Jimenez-Clavero, Miguel Angel
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Fernandez-Pinero, Jovita
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Article (Published version)
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Show full item recordAbstract
The increasing incidence of West Nile virus (WNV) in the Euro-Mediterranean area warrants the implementation of effective surveillance programs in animals. A crucial step in the fight against the disease is the evaluation of the capacity of the veterinary labs to accurately detect the infection in animal populations. In this context, the animal virology network of the MediLabSecure project organized an external quality assessment (EQA) to evaluate the WNV molecular and serological diagnostic capacities of beneficiary veterinary labs. Laboratories from 17 Mediterranean and Black Sea countries participated. The results of the triplex real time RT-PCR for simultaneous detection and differentiation of WNV lineage 1 (L1), lineage 2 (L2) and Usutu virus (USUV) were highly satisfactory, especially for L1 and L2, with detection rates of 97.9% and 100%, respectively. For USUV, 75% of the labs reported correct results. More limitations were observed for the generic detection of flaviviruses usin...g conventional reverse-transcription polymerase chain reaction (RT-PCR), since only 46.1% reported correct results in the whole panel. As regards the serological panel, the results were excellent for the generic detection of WNV antibodies. More variability was observed for the specific detection of IgM antibodies with a higher percentage of incorrect results mainly in samples with low titers. This EQA provides a good overview of the WNV (and USUV) diagnostic performance of the involved veterinary labs and demonstrates that the implemented training program was successful in upgrading their diagnostic capacities.
Keywords:
West Nile virus (WNV) / Usutu virus (USUV) / PCR / MediLabSecure / flavivirus / external quality assessment (EQA) / ELISA / diagnosticsSource:
Pathogens, 2020, 9, 12Publisher:
- MDPI, Basel
Funding / projects:
- European Commission [EU DG DEVCO: IFS/21010/23/-194, 2018/402-247]
DOI: 10.3390/pathogens9121038
ISSN: 2076-0817
PubMed: 33322276
WoS: 000602425500001
Scopus: 2-s2.0-85097681600
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Institution/Community
Naučni institut za veterinarstvo SrbijeTY - JOUR AU - Perez-Ramirez, Elisa AU - Cano-Gomez, Cristina AU - Llorente, Francisco AU - Vodica, Ani AU - Veljović, Ljubiša AU - Toklikishvilli, Natela AU - Sherifi, Kurtesh AU - Sghaier, Soufien AU - Omani, Amel AU - Kustura, Aida AU - Krstevski, Kiril AU - Karayel-Hacioglu, Ilke AU - Hagag, Naglaa Mohamed AU - El Hage, Jeanne AU - Davdyan, Hasmik AU - Bintarif, Mohd Saddam AU - Adžić, Bojan AU - Abouchoaib, Nabil AU - Jimenez-Clavero, Miguel Angel AU - Fernandez-Pinero, Jovita PY - 2020 UR - https://reponivs.nivs.rs/handle/123456789/425 AB - The increasing incidence of West Nile virus (WNV) in the Euro-Mediterranean area warrants the implementation of effective surveillance programs in animals. A crucial step in the fight against the disease is the evaluation of the capacity of the veterinary labs to accurately detect the infection in animal populations. In this context, the animal virology network of the MediLabSecure project organized an external quality assessment (EQA) to evaluate the WNV molecular and serological diagnostic capacities of beneficiary veterinary labs. Laboratories from 17 Mediterranean and Black Sea countries participated. The results of the triplex real time RT-PCR for simultaneous detection and differentiation of WNV lineage 1 (L1), lineage 2 (L2) and Usutu virus (USUV) were highly satisfactory, especially for L1 and L2, with detection rates of 97.9% and 100%, respectively. For USUV, 75% of the labs reported correct results. More limitations were observed for the generic detection of flaviviruses using conventional reverse-transcription polymerase chain reaction (RT-PCR), since only 46.1% reported correct results in the whole panel. As regards the serological panel, the results were excellent for the generic detection of WNV antibodies. More variability was observed for the specific detection of IgM antibodies with a higher percentage of incorrect results mainly in samples with low titers. This EQA provides a good overview of the WNV (and USUV) diagnostic performance of the involved veterinary labs and demonstrates that the implemented training program was successful in upgrading their diagnostic capacities. PB - MDPI, Basel T2 - Pathogens T1 - Evaluation of West Nile Virus Diagnostic Capacities in Veterinary Laboratories of the Mediterranean and Black Sea Regions IS - 12 VL - 9 DO - 10.3390/pathogens9121038 UR - conv_557 ER -
@article{ author = "Perez-Ramirez, Elisa and Cano-Gomez, Cristina and Llorente, Francisco and Vodica, Ani and Veljović, Ljubiša and Toklikishvilli, Natela and Sherifi, Kurtesh and Sghaier, Soufien and Omani, Amel and Kustura, Aida and Krstevski, Kiril and Karayel-Hacioglu, Ilke and Hagag, Naglaa Mohamed and El Hage, Jeanne and Davdyan, Hasmik and Bintarif, Mohd Saddam and Adžić, Bojan and Abouchoaib, Nabil and Jimenez-Clavero, Miguel Angel and Fernandez-Pinero, Jovita", year = "2020", abstract = "The increasing incidence of West Nile virus (WNV) in the Euro-Mediterranean area warrants the implementation of effective surveillance programs in animals. A crucial step in the fight against the disease is the evaluation of the capacity of the veterinary labs to accurately detect the infection in animal populations. In this context, the animal virology network of the MediLabSecure project organized an external quality assessment (EQA) to evaluate the WNV molecular and serological diagnostic capacities of beneficiary veterinary labs. Laboratories from 17 Mediterranean and Black Sea countries participated. The results of the triplex real time RT-PCR for simultaneous detection and differentiation of WNV lineage 1 (L1), lineage 2 (L2) and Usutu virus (USUV) were highly satisfactory, especially for L1 and L2, with detection rates of 97.9% and 100%, respectively. For USUV, 75% of the labs reported correct results. More limitations were observed for the generic detection of flaviviruses using conventional reverse-transcription polymerase chain reaction (RT-PCR), since only 46.1% reported correct results in the whole panel. As regards the serological panel, the results were excellent for the generic detection of WNV antibodies. More variability was observed for the specific detection of IgM antibodies with a higher percentage of incorrect results mainly in samples with low titers. This EQA provides a good overview of the WNV (and USUV) diagnostic performance of the involved veterinary labs and demonstrates that the implemented training program was successful in upgrading their diagnostic capacities.", publisher = "MDPI, Basel", journal = "Pathogens", title = "Evaluation of West Nile Virus Diagnostic Capacities in Veterinary Laboratories of the Mediterranean and Black Sea Regions", number = "12", volume = "9", doi = "10.3390/pathogens9121038", url = "conv_557" }
Perez-Ramirez, E., Cano-Gomez, C., Llorente, F., Vodica, A., Veljović, L., Toklikishvilli, N., Sherifi, K., Sghaier, S., Omani, A., Kustura, A., Krstevski, K., Karayel-Hacioglu, I., Hagag, N. M., El Hage, J., Davdyan, H., Bintarif, M. S., Adžić, B., Abouchoaib, N., Jimenez-Clavero, M. A.,& Fernandez-Pinero, J.. (2020). Evaluation of West Nile Virus Diagnostic Capacities in Veterinary Laboratories of the Mediterranean and Black Sea Regions. in Pathogens MDPI, Basel., 9(12). https://doi.org/10.3390/pathogens9121038 conv_557
Perez-Ramirez E, Cano-Gomez C, Llorente F, Vodica A, Veljović L, Toklikishvilli N, Sherifi K, Sghaier S, Omani A, Kustura A, Krstevski K, Karayel-Hacioglu I, Hagag NM, El Hage J, Davdyan H, Bintarif MS, Adžić B, Abouchoaib N, Jimenez-Clavero MA, Fernandez-Pinero J. Evaluation of West Nile Virus Diagnostic Capacities in Veterinary Laboratories of the Mediterranean and Black Sea Regions. in Pathogens. 2020;9(12). doi:10.3390/pathogens9121038 conv_557 .
Perez-Ramirez, Elisa, Cano-Gomez, Cristina, Llorente, Francisco, Vodica, Ani, Veljović, Ljubiša, Toklikishvilli, Natela, Sherifi, Kurtesh, Sghaier, Soufien, Omani, Amel, Kustura, Aida, Krstevski, Kiril, Karayel-Hacioglu, Ilke, Hagag, Naglaa Mohamed, El Hage, Jeanne, Davdyan, Hasmik, Bintarif, Mohd Saddam, Adžić, Bojan, Abouchoaib, Nabil, Jimenez-Clavero, Miguel Angel, Fernandez-Pinero, Jovita, "Evaluation of West Nile Virus Diagnostic Capacities in Veterinary Laboratories of the Mediterranean and Black Sea Regions" in Pathogens, 9, no. 12 (2020), https://doi.org/10.3390/pathogens9121038 ., conv_557 .