Phylogenetic analysis of ha and na genes of swine influenza viruses in Serbia in 2016-2018
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2020
Authors
Maksimović Zorić, Jelena![](/themes/Miragereponivs/images/orcid.png)
Milićević, Vesna
![](/themes/Miragereponivs/images/orcid.png)
Stevančević, Ognjen
![](/themes/Miragereponivs/images/orcid.png)
Chiapponi, Chiara
Potkonjak, Aleksandar
![](/themes/Miragereponivs/images/orcid.png)
Stojanac, Nenad
![](/themes/Miragereponivs/images/orcid.png)
Kureljušić, Branislav
![](/themes/Miragereponivs/images/orcid.png)
Veljović, Ljubiša
![](/themes/Miragereponivs/images/orcid.png)
Radosavljević, Vladimir
![](/themes/Miragereponivs/images/orcid.png)
Savić, Božidar
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Article (Published version)
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Pigs are very important for the epidemiology of influenza A viruses, being commonly infected with the lineages of most adapted H1N1, H3N2, H1N2 swine subtypes. Epidemiological complexity of swine influenza is increasing by a periodic spillover of human or avian viruses in the pig population when genetic shifts can occur. The objectives of this research were to determine the presence of the influenza A virus in nasal and tracheobronchial swabs and lung tissue samples of ill and dead pigs on commercial farms, to determine circulating subtypes and characterize them through the phylogenetic analysis of hemagglutinin (HA) and neuraminidase (NA) genes. A total of 255 samples collected from 13 farms were analyzed by means of real-time RTPCR. The genome of influenza A virus was detected in 24 samples, which represented a 61.5% prevalence at the farms level (influenza A virus was confirmed in 8 out of 13 farms included in this study). Based on HA and NA gene sequences of 8 viruses, the circulat...ion of H1N1 and H3N2 subtypes of influenza A viruses were determined. In addition, one farm exhibited a time separated circulation of H1N1 and H3N2 virus subtypes. Using Influenza Research Database, our viruses of the H1 subtype were classified into 1C.2.1 and 1A.3.3.2. Glade. Based on the nucleotide sequences of HA genes, three viruses of the H1N1 subtype belong to the H1N1pdm09 lineage, and the other four to Eurasian "avian-like" H1avN1 lineage; while based on NA genes sequences, these seven viruses belong to Eurasian "avian-like" H1avN1 lineage. Both HA and NA genes of the virus of the H3N2 subtype belonged to the A/swine/Gent/1/1984-like H3N2 lineage.
Keywords:
swine / subtypes / lineages / influenza A virusesSource:
Acta Veterinaria-Beograd, 2020, 70, 1, 110-125Publisher:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Funding / projects:
- Institute of Veterinary Medicine of Serbia, Republic of Serbia
- Wild animal health monitoring and introduction of new biotechnology procedures in detection of infectious and zoonotic agents - risk analysis for human health, domestic and wild animal health and for environmental contamination (RS-MESTD-Technological Development (TD or TR)-31084)
- Ecological and viral investigation on the presents of emerging zoonoses in national parks in republic of Serbia (RS-MESTD-Technological Development (TD or TR)-37015)
- Improvement and development of hygienic and technological procedures in production of animal originating foodstuffs with the aim of producing high-quality and safe products competetive on the global market (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46009)
DOI: 10.2478/acve-2020-0008
ISSN: 0567-8315
WoS: 000525346500008
Scopus: 2-s2.0-85083800473
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Institution/Community
Naučni institut za veterinarstvo SrbijeTY - JOUR AU - Maksimović Zorić, Jelena AU - Milićević, Vesna AU - Stevančević, Ognjen AU - Chiapponi, Chiara AU - Potkonjak, Aleksandar AU - Stojanac, Nenad AU - Kureljušić, Branislav AU - Veljović, Ljubiša AU - Radosavljević, Vladimir AU - Savić, Božidar PY - 2020 UR - https://reponivs.nivs.rs/handle/123456789/406 AB - Pigs are very important for the epidemiology of influenza A viruses, being commonly infected with the lineages of most adapted H1N1, H3N2, H1N2 swine subtypes. Epidemiological complexity of swine influenza is increasing by a periodic spillover of human or avian viruses in the pig population when genetic shifts can occur. The objectives of this research were to determine the presence of the influenza A virus in nasal and tracheobronchial swabs and lung tissue samples of ill and dead pigs on commercial farms, to determine circulating subtypes and characterize them through the phylogenetic analysis of hemagglutinin (HA) and neuraminidase (NA) genes. A total of 255 samples collected from 13 farms were analyzed by means of real-time RTPCR. The genome of influenza A virus was detected in 24 samples, which represented a 61.5% prevalence at the farms level (influenza A virus was confirmed in 8 out of 13 farms included in this study). Based on HA and NA gene sequences of 8 viruses, the circulation of H1N1 and H3N2 subtypes of influenza A viruses were determined. In addition, one farm exhibited a time separated circulation of H1N1 and H3N2 virus subtypes. Using Influenza Research Database, our viruses of the H1 subtype were classified into 1C.2.1 and 1A.3.3.2. Glade. Based on the nucleotide sequences of HA genes, three viruses of the H1N1 subtype belong to the H1N1pdm09 lineage, and the other four to Eurasian "avian-like" H1avN1 lineage; while based on NA genes sequences, these seven viruses belong to Eurasian "avian-like" H1avN1 lineage. Both HA and NA genes of the virus of the H3N2 subtype belonged to the A/swine/Gent/1/1984-like H3N2 lineage. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - Phylogenetic analysis of ha and na genes of swine influenza viruses in Serbia in 2016-2018 EP - 125 IS - 1 SP - 110 VL - 70 DO - 10.2478/acve-2020-0008 UR - conv_364 ER -
@article{ author = "Maksimović Zorić, Jelena and Milićević, Vesna and Stevančević, Ognjen and Chiapponi, Chiara and Potkonjak, Aleksandar and Stojanac, Nenad and Kureljušić, Branislav and Veljović, Ljubiša and Radosavljević, Vladimir and Savić, Božidar", year = "2020", abstract = "Pigs are very important for the epidemiology of influenza A viruses, being commonly infected with the lineages of most adapted H1N1, H3N2, H1N2 swine subtypes. Epidemiological complexity of swine influenza is increasing by a periodic spillover of human or avian viruses in the pig population when genetic shifts can occur. The objectives of this research were to determine the presence of the influenza A virus in nasal and tracheobronchial swabs and lung tissue samples of ill and dead pigs on commercial farms, to determine circulating subtypes and characterize them through the phylogenetic analysis of hemagglutinin (HA) and neuraminidase (NA) genes. A total of 255 samples collected from 13 farms were analyzed by means of real-time RTPCR. The genome of influenza A virus was detected in 24 samples, which represented a 61.5% prevalence at the farms level (influenza A virus was confirmed in 8 out of 13 farms included in this study). Based on HA and NA gene sequences of 8 viruses, the circulation of H1N1 and H3N2 subtypes of influenza A viruses were determined. In addition, one farm exhibited a time separated circulation of H1N1 and H3N2 virus subtypes. Using Influenza Research Database, our viruses of the H1 subtype were classified into 1C.2.1 and 1A.3.3.2. Glade. Based on the nucleotide sequences of HA genes, three viruses of the H1N1 subtype belong to the H1N1pdm09 lineage, and the other four to Eurasian "avian-like" H1avN1 lineage; while based on NA genes sequences, these seven viruses belong to Eurasian "avian-like" H1avN1 lineage. Both HA and NA genes of the virus of the H3N2 subtype belonged to the A/swine/Gent/1/1984-like H3N2 lineage.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "Phylogenetic analysis of ha and na genes of swine influenza viruses in Serbia in 2016-2018", pages = "125-110", number = "1", volume = "70", doi = "10.2478/acve-2020-0008", url = "conv_364" }
Maksimović Zorić, J., Milićević, V., Stevančević, O., Chiapponi, C., Potkonjak, A., Stojanac, N., Kureljušić, B., Veljović, L., Radosavljević, V.,& Savić, B.. (2020). Phylogenetic analysis of ha and na genes of swine influenza viruses in Serbia in 2016-2018. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 70(1), 110-125. https://doi.org/10.2478/acve-2020-0008 conv_364
Maksimović Zorić J, Milićević V, Stevančević O, Chiapponi C, Potkonjak A, Stojanac N, Kureljušić B, Veljović L, Radosavljević V, Savić B. Phylogenetic analysis of ha and na genes of swine influenza viruses in Serbia in 2016-2018. in Acta Veterinaria-Beograd. 2020;70(1):110-125. doi:10.2478/acve-2020-0008 conv_364 .
Maksimović Zorić, Jelena, Milićević, Vesna, Stevančević, Ognjen, Chiapponi, Chiara, Potkonjak, Aleksandar, Stojanac, Nenad, Kureljušić, Branislav, Veljović, Ljubiša, Radosavljević, Vladimir, Savić, Božidar, "Phylogenetic analysis of ha and na genes of swine influenza viruses in Serbia in 2016-2018" in Acta Veterinaria-Beograd, 70, no. 1 (2020):110-125, https://doi.org/10.2478/acve-2020-0008 ., conv_364 .