The prevalence of pasteurella multocida from farm pigs in Serbia
Apstrakt
The investigations covered a total of 234 lungs from necropsied pigs with different pneumonic lesions, from 6 farrow-to-finish pig farms during 2013 and 2014. The samples were inoculated on selective culture media and aerobically incubated at 37 degrees C and in carbon dioxide condition. The isolated bacterial colonies were further characterised morphologically and biochemically. The identification was confirmed using the BBL Crystal, E/N, G/P ID Kit (Becton Dickinson). For determination of the type of Pasteurella multocida, the PCR method was used. The findings showed that bacteria were isolated from 202 (86%) out of 234 examined lung samples. The pure isolates of Pasteurella multocida were obtained from 71 (35 %) samples. Out of the remaining 29 (14%) examined lung samples, 9, 8, 7 and 5 examined lung samples were shown as mixed cultures of Pasteurella multocida and Streptococcus spp., Arcanobacterium pyogenes, Actinobacillus pleuropneumoniae and Haemophilus parasuis, respectively. T...he PCR method confirmed that all 15 investigated strains of P. multocida belong to type A.
Ključne reči:
pig / Pasteurella multocida / lungIzvor:
Macedonian Veterinary Review, 2016, 39, 1, 39-42Izdavač:
- Univ Sv Kiril & Metodij Skopje, Fak Veterinarna Medicina, Skopje
DOI: 10.1515/macvetrev-2015-0063
ISSN: 1409-7621
WoS: 000375090800005
Scopus: 2-s2.0-84961168893
Institucija/grupa
Naučni institut za veterinarstvo SrbijeTY - JOUR AU - Radanović, Oliver AU - Žutić, Jadranka AU - Jakić-Dimić, Dobrila AU - Kureljušić, Branislav AU - Savić, Božidar PY - 2016 UR - https://reponivs.nivs.rs/handle/123456789/310 AB - The investigations covered a total of 234 lungs from necropsied pigs with different pneumonic lesions, from 6 farrow-to-finish pig farms during 2013 and 2014. The samples were inoculated on selective culture media and aerobically incubated at 37 degrees C and in carbon dioxide condition. The isolated bacterial colonies were further characterised morphologically and biochemically. The identification was confirmed using the BBL Crystal, E/N, G/P ID Kit (Becton Dickinson). For determination of the type of Pasteurella multocida, the PCR method was used. The findings showed that bacteria were isolated from 202 (86%) out of 234 examined lung samples. The pure isolates of Pasteurella multocida were obtained from 71 (35 %) samples. Out of the remaining 29 (14%) examined lung samples, 9, 8, 7 and 5 examined lung samples were shown as mixed cultures of Pasteurella multocida and Streptococcus spp., Arcanobacterium pyogenes, Actinobacillus pleuropneumoniae and Haemophilus parasuis, respectively. The PCR method confirmed that all 15 investigated strains of P. multocida belong to type A. PB - Univ Sv Kiril & Metodij Skopje, Fak Veterinarna Medicina, Skopje T2 - Macedonian Veterinary Review T1 - The prevalence of pasteurella multocida from farm pigs in Serbia EP - 42 IS - 1 SP - 39 VL - 39 DO - 10.1515/macvetrev-2015-0063 UR - conv_459 ER -
@article{ author = "Radanović, Oliver and Žutić, Jadranka and Jakić-Dimić, Dobrila and Kureljušić, Branislav and Savić, Božidar", year = "2016", abstract = "The investigations covered a total of 234 lungs from necropsied pigs with different pneumonic lesions, from 6 farrow-to-finish pig farms during 2013 and 2014. The samples were inoculated on selective culture media and aerobically incubated at 37 degrees C and in carbon dioxide condition. The isolated bacterial colonies were further characterised morphologically and biochemically. The identification was confirmed using the BBL Crystal, E/N, G/P ID Kit (Becton Dickinson). For determination of the type of Pasteurella multocida, the PCR method was used. The findings showed that bacteria were isolated from 202 (86%) out of 234 examined lung samples. The pure isolates of Pasteurella multocida were obtained from 71 (35 %) samples. Out of the remaining 29 (14%) examined lung samples, 9, 8, 7 and 5 examined lung samples were shown as mixed cultures of Pasteurella multocida and Streptococcus spp., Arcanobacterium pyogenes, Actinobacillus pleuropneumoniae and Haemophilus parasuis, respectively. The PCR method confirmed that all 15 investigated strains of P. multocida belong to type A.", publisher = "Univ Sv Kiril & Metodij Skopje, Fak Veterinarna Medicina, Skopje", journal = "Macedonian Veterinary Review", title = "The prevalence of pasteurella multocida from farm pigs in Serbia", pages = "42-39", number = "1", volume = "39", doi = "10.1515/macvetrev-2015-0063", url = "conv_459" }
Radanović, O., Žutić, J., Jakić-Dimić, D., Kureljušić, B.,& Savić, B.. (2016). The prevalence of pasteurella multocida from farm pigs in Serbia. in Macedonian Veterinary Review Univ Sv Kiril & Metodij Skopje, Fak Veterinarna Medicina, Skopje., 39(1), 39-42. https://doi.org/10.1515/macvetrev-2015-0063 conv_459
Radanović O, Žutić J, Jakić-Dimić D, Kureljušić B, Savić B. The prevalence of pasteurella multocida from farm pigs in Serbia. in Macedonian Veterinary Review. 2016;39(1):39-42. doi:10.1515/macvetrev-2015-0063 conv_459 .
Radanović, Oliver, Žutić, Jadranka, Jakić-Dimić, Dobrila, Kureljušić, Branislav, Savić, Božidar, "The prevalence of pasteurella multocida from farm pigs in Serbia" in Macedonian Veterinary Review, 39, no. 1 (2016):39-42, https://doi.org/10.1515/macvetrev-2015-0063 ., conv_459 .